Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Department of Blood Sciences The Princess Alexandra Hospital Hamstel Road Harlow CM20 1QX Contact: Eva Nkansah Tel: +44 (0) ext Website: Testing performed at the above address only DETAIL OF ACCREDITATION HUMAN BODY FLUIDS Biochemistry examination activities diagnosis: Abbott architect Ci16000 Detected by Colourmetric/Spectrophometry Blood Alkaline phosphatase LP Bilirubin (total and direct) LP , LP Alanin amino transferase LP , LP Albumin LP Aspartate amino transferase LP Creatine kinase LP Gamma GT LP Gentamicin LP Lactate LP Lactate dehydrogenase LP Lithium LP Detected by Enzymatic end point Ammonia LP CholesterFol, triglyceride, HDL LP , LP Bile Acid LP Magnesium LP Paracetamol LP Salicylate LP Theophyllin LP Detected by Immunoturbometry Total IgE LP Rheumatoid factor LP Alpha 1 antitrypsin LP Beta 2 microglobulin LP C3 & C4 LP , LP CRP LP Digoxin LP Assessment Manager: AB2 Page 1 of 9

2 Biochemistry examination activities methods for Abbott architect Ci16000 (cont d) Detected by Immunoturbometry Blood (cont d) IgA, IgM, IgG LP , LP , LP Iron LP Vancomycin LP Valproate LP Transferin LP Detected by ChemiIlluminescene Assay (CMIA) HS troponin I LP Carbamazepine LP Alphafeto protein LP Vitamin B12 LP Beta HCG LP Ca125 LP CA153 LP CEA LP Cortisol LP Ferritin LP Folate LP FSH LP FT3 LP FT4 LP LH LP Prolactin LP Macroprolactin (PEG ppt) LP a Oestradiol LP Phenytoin LP Progesterone LP PSA LP Testosterone LP TSH LP Vitamin D LP Detected by Immunoturbometry Urine Microalbumin LP Detected by Indirect ISE Blood and Urine Sodium LP , LP Potassium LP , LP Chloride LP , LP , LP Assessment Manager: AB2 Page 2 of 9

3 Biochemistry examination activities methods for Abbott architect Ci16000 (cont d) Detected by Colourmetric/Spectrophometry Blood and Urine Calcium LP , LP Creatinine LP , LP Phosphate LP , LP Amylase LP Blood, urine, and CSF Protein LP , LP , LP Detected by Enzymatic end point Blood and Urine Urea LP , LP Uric acid LP Blood and CSF Glucose LP , LP Calculations in the LIMS Blood/Serum LDL LP , egfr LP , Corrected calcium LP , Globulin LP , Transferrin saturation LP Sweat Conductivity Sweat Chek conductivity meter LP Blood and urine Detection of normal and abnormal Immunoglobulin bands Protein electrophoresis and Immunotyping using the Sebia Capillarys & Hydrasys separation system. LP LP Blood HbA1c diabetic monitoring Using Bio Ra variant II turbo. Ion Exchange chromatography LP Blood NT pro BNP Roche Cobas E411. Chemiluminescence immunoassay. LP Assessment Manager: AB2 Page 3 of 9

4 Blood Biochemistry examination activities Anti-tissue Transglutaminase IgA screening for coeliac disease ANA screening methods DS2 Launch. Enzyme Immunoassay. L LP Blood and Urine Osmolality Vitec scientific. Freezing point depression LP CSF Xanthochromia Uvikon spectrophotometer. LP Faeces Faecal Occult Blood MAST Occutest Manufactured slide test- manual detection of blood by colour reaction LP Assessment Manager: AB2 Page 4 of 9

5 Haematology examination activities diagnosis: Plasma Coagulation studies: ACL TOP Analyser LP LP Coag screen: Prothrombin time/inr APTT Factor XIII Fibrinogen assay Inhibitor screen: APTT 50:50 LP LP LP LP Lupus screen ratio: Diluted Russell s Viper Venom Test (DRVVT screen) APTT-sp Lupus sensitive D-Dimer LP LP Latex agglutination method LP Anti Xa assay, Thrombophilia screen Protein C Protein S Thrombin time APCR Factors II / V / VII / X Chromogenic assay LP LP Factor IX / VIII / XI / XII LP Assessment Manager: AB2 Page 5 of 9

6 Blood Blood Blood film slides Bone Marrow aspirates Blood Haematology examination activities Infectious mononucleosis screen for Paul Bunnell Infectious Mononucleosis heterophile antibodies Haemoglobinopathy screening for Thalassemias & Haemoglobin variants: Hb S,C,D,E Beta Thalassaemia trait Identification of normal and abnormal morphologies including WBC differential count Reporting of normal and abnormal morphologies Screening for the detection of Plasmodium falciparum and Plasmodium spp. including: Plasmodium ovale Plasmodium falciparum Plasmodium malariae Plasmodium vivax Detection and Identification of Plasmodium species: P.falciparum P.vivax P.ovale P.malariae P knowlesi Accusay Lateral Flow immunoassay LP BIO-RAD Variant II analyser. HPLC LP Using Hematek stainer and manual microscopy LP LP Using Hematek stainer and manual microscopy LP Manual Perl s stain LP Humasis Malaria P.F/Pan antigen test LP Giemsa stained thin films Field s stain thick films Manual staining LP Assessment Manager: AB2 Page 6 of 9

7 Haematology examination activities Blood HbS Microgen S-test kit LP Blood ESR Vitech Starssed Compact analyser using Westergren sedimentation method. Detectetion of plasma/cells interface LP Blood FBC: Sysmex XN analysers: 1 x XN2000 and 1 x XN1000 Fluorescence Flow Cytometry for WBC Hydrodynamic Focussing (aperture Impedance) for RBC and Platelets. Sodium Laurly-Sulphate (cyanide free) for HGB analysis. LP LP Haemoglobin estimation Red cell and platelet counts Haematocrit Differential white cell count MCV MCH MCHC Reticulocytes colourmetric Electrical impedence By calculation Elecrical impedence Assessment Manager: AB2 Page 7 of 9

8 Whole blood in EDTA Blood Transfusion examination activities diagnosis: Blood groups. ABO and RhD systems Antibody screening and identification: Rh blood group antibodies: Anti-Cw, C, c, E, e and D. Kell blood group antibodies: Anti- Kell, k, Kpa and Kpb. Kidd blood group antibodies: Anti- Jka and Jkb. Duffy blood group antibodies: Anti- Fya, and Fyb. Lutheran blood group antibodies: Anti-Lua and Lub. MNS blood group antibodies: Anti- M, N, S and s. Lewis blood group antibodies: Anti- Lea and Leb. P blood group antibodies: Anti-P1 Serological crossmatch and compatibility testing Automated method using Ortho Visionblood group analyser. LP Manual method using tube techniques and Lorne reagents. LP Automated screening method using Ortho Vision blood group analyser. LP Manual screening method using BioVue column gel technology and equipment. Biovue and NHSBT cells LP Manual identification method using BioVue column gel technology and equipment. LP Manual antenatal 4 cell screening method using BioVue column gel technology and equipment. All antibodies except anti-d LP Manual method using BioVue column gel technology and equipment. LP Assessment Manager: AB2 Page 8 of 9

9 Blood Transfusion examination activities Whole blood in EDTA Neonatal direct antiglobulin test Automated method using BioVue neonatal cards on OrthoVision analyser LP Manual method using BioVue neonatal card LP Direct antiglobulin test: detection of Anti-IgG and C3d Red cell phenotyping for antigens of the following blood group systems: Rh: C, D, E, c, e, and Cw Kell: K and k BioVue monspecific cards. LP Manual method using tube techniques and Lorne reagents. BioVue AHG cards and Lorne reagents. LP Whole blood Duffy: Fya and Fyb Kidd: Jka and Jkb MNS: M, N, S and s Lewis: Lea ABO: A1 Foetal/maternal haemorrhage screen (Kleihauer) Clin-tech Shepards stain reagent. LP END Assessment Manager: AB2 Page 9 of 9

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