Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Haematology & Blood Transfusion Contact: Neil Wrathall The Christie Tel: +44 (0) Wilmslow Road Fax: +44 (0) Manchester M20 4BX Website: Testing performed at the above address only Site activities performed away from the locations listed above: Location details Activity Address Haematology & Blood Transfusion The Christie Wilmslow Road Manchester M20 4BX Local contact Contact: Dr. Neil Wrathall Tel: +44 (0) Fax: +44 (0) Theatre Blood Issue Fridge Assessment Manager: GS2 Page 1 of 6

2 DETAIL OF ACCREDITATION Venous blood: citrated Bone marrow aspirate Cerebrospinal fluid Haematological examination activities for the purpose of clinical diagnosis Haemoglobin (HB) White Blood Cells (WBC) Platelets (PLTS) Red blood cells (RBC) Haematocrit (HCT) Mean Cell Volume (MCV) Mean Cell Haemoglobin (MCH) Mean cell haemoglobin concentration (MCHC) Red Cell Distribution (RDW) Neutrophils (NEUT) Lymphocytes (LYMP) Eosinophils (EOSO) Basophils (BASO) Monocytes (MONO) Large unstained cells (LUC) Reticulocyte enumeration Platelet count Blood film assessment (peripheral) for detection of abnormalities and cell differential. Bone marrow film for the detection of normal and abnormal morphology by microscopic examination CSF cell count and cytospin for the detection of normal and abnormal morphology by microscopic examination manufacturer s instructions Advia 2120i (flow cytometry and laser light refraction ) with reference to: LI-Haem-Christie-Advia Startup and Processing Hematek 2000 stainer modified Wright stain and Nikon microscope. Manual assessment with ref to: LI-Haem-Christie-Bloodfilm exam LI-Haem-Christie-BMSmears LI-Haem-Christie-BMReports Manual cell count, modified Wright stain with ref to : LI-Haem-CPP-CSF Assessment Manager: GS2 Page 2 of 6

3 (cont) Ascitic/pleural fluid Venous blood: citrated Haematological examination activities for the purpose of clinical diagnosis (cont d) Fluid cytospin for the detection of normal and abnormal morphology by microscopic examination Detection/screening for Plasmodium spp. Erythrocyte sedimentation Rate (ESR) manufacturer s instructions Manual cell count, modified Wright stain with ref to: LI-Haem-CPP CSF Manual method, modified Wright stain with reference to: LI-Haem-Christie-Field Stain Manual method - Sedimentation with reference to : LI-Haem Christie -ESR Sickle cell solubility Solubility screening test, TCS sickle check screening kit with reference to : LI-Haem Christie-sickletest and Bone Marrow Urine Citrated Iron levels Detection of Urinary Haemosiderin Coagulation Screen: Prothrombin time (PT) Activated partial thromboplastin time (APTT) Derived Fibrinogen Clauss Fib Thrombin time Reptilase time D-Dimer 50/50 Basic correction test FX assay Qualitative assessment using Pearls Prussian blue stain and Nikon Microscope with reference to: LI-Haem-Christie-perls LI-Haem-Christie-haemosiderin using Pearls Prussian blue (iron ) stain with ref to : LI-Haem- Christie-perls Werfen ACL TOP (50 series) with reference to: LI-Haem-Christie-ACL TOP 350 Analyser Routine Operation Assessment Manager: GS2 Page 3 of 6

4 (cont), cerebrospinal fluid and bone marrow aspirate. Haematological examination activities for the purpose of clinical diagnosis (cont d) Diagnosis and Monitoring of Haematological malignanciesdifferential assessment of surface antigens and cytoplasmic antigens using flow cytometry Identification of: manufacturer s instructions Navios/FC500 (fluorochrome labelled antibody expression ) with reference to : LI-Haem-CPP-Navios Acute-intra panels LI-Haem-CPP-Navios CLL Panel LI-Haem-Christie-FC500 Acute-Intra Panel LI-Haem-CPP-Christie-FC500CLL Panel Endothelial cells, blast cell CD34 or Peripheral Stem Cells in EDTA or Peripheral Stem Cells in EDTA or Peripheral Stem Cells in EDTA / T-helper cells and related lymphoma Mature suppressor / cytotoxic T-cells, associated T-cell lymphomas Pan T-cell marker & T-cell lymphomas Blood Transfusion Tests ABO and Rhd system investigation. O RhD positive A RhD positive B RhD positive AB RhD positive O RhD negative A RhD negative B RhD negative AB RhD negative Antibody Screen Rhesus blood group antibodies: anti-cw, anti-c, anti-c, anti-d, anti-e, anti-e CD4 CD8 CD3 Diamed ID Gel Station (immunohaematology) with reference to: LI-BB-CPP-DiamedID Gelstation Assessment Manager: GS2 Page 4 of 6

5 (cont d) / Plasma/ granulocytes/buffy coat / Blood Transfusion Tests (cont d) MNS blood group antibodies: anti-m, anti-n, anti-s, anti-s P blood group antibodies: anti- P1 - Lutheran blood group antibodies: anti-lua, Lewis blood group antibodies: anti-lea, anti-leb Kell system blood group antibodies: anti-k, anti-k, anti- Kpa Duffy blood group antibodies: anti-fya, anti-fyb Kidd blood group antibodies: anti-jka, anti-jkb Antiglobulin test Manual group and Screen Quick blood group Direct Antiglogulin test Serological Cross match Emergency crossmatch manufacturer s instructions: Diamed ID Gel Station (immunohaematology) with reference to: LI-BB-CPP-DiamedID Gelstation LI-BB-Christie-Direct Coombs test Diamed Diaclon cards with reference to : LI-BB-Christie-G&S LI-BB-Christie-QG Diamed LISS/Coombs cards with reference to LI-BB-Christie-Direct Coombs test Diamed LISS Coombs cards, Diaclon card and Diamed reagents With reference to: LI-BB-Christie-Gel Column cross match Diamed LISS Coombs cards, Diaclon card and Diamed reagents With reference to: LI-BB-Christie-Gel Column cross match Assessment Manager: GS2 Page 5 of 6

6 (cont) / Blood Transfusion Tests (cont d) Antibody panels antigens of the following systems: Rh C,D,E,c,e,Cw MNSs M,N,S,s P P1 Lutheran Lua Kell K,k,Kpa Lewis Lea, Leb Duffy Fya, Fyb Kidd Jka, Jkb manufacturer s instructions: Manual Diamed LISS/Coombs cards and NaCl enzyme cards with reference to: LI-BB-Christie-cell panels END Assessment Manager: GS2 Page 6 of 6