Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK University Hospitals NHS Foundation Trust Haematology and Transfusion Department Mindelsohn Way Edgbaston Contact: Tel: +44 (0) Website: Additional site activities performed at main location listed above: Location details Activity ED RESUS AREA LEVEL 1 CRITICAL CARE D LEVEL 2 CRITICAL CARE A LEVEL 2 MAIN THEATRE LEVEL 2 THEATRE 3 LEVEL 2 WARD 621 LEVEL 6, WARD 625 LEVEL 6 Assessment Manager: DE 1 of 7

2 University Hospitals NHS Foundation Trust Location details Activity Welcome Theatre 2 nd Floor Heritage Building Ambulatory Care Area Level 1 Site activities performed away from the main laboratory location listed above: Location details Activity Royal Orthopaedic Hospital B31 2AP Satellite blood issue fridge for storage of blood Assessment Manager: DE Page 2 of 7

3 University Hospitals NHS Foundation Trust DETAIL OF ACCREDITATION Haematology examination activities for : Full Blood Count Includes the following parameters Haemoglobin (HGB) Red Blood Cell Count (RBC) White Blood Cell Count (WBC) Platelet Count (PLT) Haematocrit (HCT) Total White Blood Cell Count & 5 part differential(diff) Mean Cell Haemoglobin (MCH) Mean Cell Volume (MCV) Mean Cell Haemoglobin Concentration (MCHC) Beckman Coulter DxH 800. Spectrophotometric, impedance and flow cytometric analysis. HRA_S070 Reticulocyte count Beckman Coulter DxH 800. New methylene blue staining and flow cytometric analysis. HRA_S070 Erythrocyte Sedimentation Rate Alifax ESR analyser. Micro aggregation method. HRA_S071 Test results from the Biochemistry laboratory. Haemoglobin Variant analysis. Haemoglobin A2 percentage. Haemoglobin F percentage Haemoglobin S percentage B12 Folate Ferritin TOSOH G8. HPLC:HHH_S080 Cobas 8000 system. Roche immunoassay. Sandwich assay CRA_S139 CRA_S135 CRA_S134 Haemoglobin S detection (HbS) TCS Biosciences Sickle-Check kit. Solubility test. HHH_S035 Malarial antigens for: Plasmodium falciparum and pan-detection for: Plasmodium malariae, Plasmodium ovale & Plasmodium vivax Rapydtest Carestart kit. Antigen detection. HRA_S014 Assessment Manager: DE Page 3 of 7

4 University Hospitals NHS Foundation Trust Haematology examination activities for Malaria Parasite detection and speciation P. falciparum P. ovale P. vivax P malariae Blood Film analysis for confirmation of FBC results; manual differential if applicable, white, red and platelet number assessment and morphology. Glandular Fever Screen detection of infectious mononucleosis heterophile antibodies Coagulation examination activities for : Microscopic analysis: Manual slide making and staining by thin film. HRA_S014 Beckman Coulter SMS. Slide maker and stainer. HRA_S073 Microscopic analysis. HRA_S068 Accusay, immunoassay HRA_S013 PTT, aptt with 50% NP ACL TOP 700: Turbidimetric clot detection measurement. HRA_S056 Prothrombin (Factor II), Factor V, VII, VIII, IX, X, XI, XII F.VIII pre treatment, post treatment F.IX pre treatment, post treatment Turbidimetric clot detection measurement. HHH_S074 Fibrinogen Turbidimetric clot detection measurement. HRA_S057 F.VIII inhibitor (H) Turbidimetric clot detection measurement. HHH_S004 INR PT PT with 50% NP Turbidimetric clot detection measurement. HRA_S055 Inhibitor Screen Turbidimetric clot detection measurement. HHH_S084 Lupus Anticoagulant Turbidimetric clot detection measurement. HHH_S069 Assessment Manager: DE Page 4 of 7

5 University Hospitals NHS Foundation Trust Coagulation examination activities for ACL TOP 700 Antithrombin function Chromogenic measurement. HHH_S067 Protein C function Chromogenic measurement. HRA_S068 Free Protein S antigen Latex particle immunoturbidimetric measurement. HHH_S078 D-Dimer Immunological measurement. HRA_S058 Thrombin Time Turbidimetric clot detection measurement. HHH_S018 VWF:Ristocetin Cofactor Activity pre treatment, post treatment VWF:Ag Antigen pre treatment, post treatment Anti-Xa: Unfractionated Heparin(UFH) Low Molecular Weight Heparin(LMWH) Rivaroxaban Apixaban Fondaparinux (Arixtra) Danaparoid (Orgaran) Latex particle immunoturbidimetric measurement. HHH_S083 Latex particle immunoturbidimetric measurement. HHH_S082 ACL TOP 700, LAS TOP 700: Chromogeneic measurement: HHH_S086 Prothrombin Time: PT Manual Method. Waterbath HRA_S001 Activated Partial Thromboplastin Time: APTT Manual Method. Waterbath HRA_S002 Thrombin Time Manual Method. Waterbath HRA_S080 VWF Collagen Binding Manual Method: ELISA HHH_S087 Assessment Manager: DE Page 5 of 7

6 University Hospitals NHS Foundation Trust Coagulation examination activities for Venous Blood: Serum Venous Blood; plasma Heparin Induced Thrombocytopenia screening (HIT) ADAMTS_13 activity ADAMTS_13 inhibitor Molecular detection of: Factor V Leiden DNA Prothrombin 20210A DNA Transfusion examination activities for : ABO Blood Group, Rhesus typing and antibody screen automated Detection of antigens ABO and RhD groups Screening of antibodies Anti-D, C, E, c, e, Cw, K, k, Kp(a), Kp(b), Fy(a), Fy(b), Jk(a), Jk(b), Le(a), Le(b), P1, M, N, S, s, Lu(a) and Lu(b). ABO Blood Group, Rhesus typing and antibody screen automated Detection of antigens ABO and RhD groups Screening of antibodies anti-d, C, E, c, e, Cw, K, k, Kp(a), Kp(b), Fy(a), Fy(b), Jk(a), Jk(b), Le(a), Le(b), P1, M, N, S, s, Lu(a) and Lu(b). Enzyme panel, Indirect Antiglobulin Test panel Identification of antibodies: Anti-D, C, E, c, e, Cw, K, k, Kp(a), Kp(b), Fy(a), Fy(b), Jk(a), Jk(b), Le(a), Le(b), P1, M, N, S, s, Lu(a) and Lu(b). Manual Method: ELISA HHH_S085 Manual Method: ELISA HHH_S088 Manual Method: ELISA HHH_S089 Cepheid GeneXpert HHH_S030 IBG Neo Analyser. Capture antibody detection system and micro titre liquid phase grouping plate technology HBB_S119 Bio-rad system. Column agglutination technology: HBB_S129 HBB_S131 Venous Blood; plasma Phenotype of antigens HBB_S134 Assessment Manager: DE Page 6 of 7

7 University Hospitals NHS Foundation Trust Transfusion examination activities for Direct Antiglobulin Test HBB_S133 Crossmatch Compatibility testing between donor red cell antigens and patient plasma antibodies. Rapid group forward group with anti-a,anti-b and anti-d Manual Tube Quick Spin Crossmatch HBB_S034 BIO-RAD Manual Crossmatch HBB_S130 Manual technique. Tile agglutination. HBB_S008 A sub type Manual technique. Tube agglutination. HBB_S070 END Assessment Manager: DE Page 7 of 7

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