New genomic typing method MLST

Transcription

1 New genomic typing method MLST Bon KIMURA fingerprinting PFGE DNA multilocus sequence typingmlst alleles PFGE MLST 1990 PCR 1 PCR DNA PFGE 1 PFGE RAPDrandomly amplified polymorphic DNA 3 AFLPAmplified fragment length polymorphism 4 Ribotyping 5 multilocus sequence typingmlst Laboratory of Food Microbiology, Department of Food Science and Technology, Faculty of Marine Science, Tokyo University of Marine Science and Technology (4-5-7 Konan, Minato-ku, Tokyo)

2 PFGE PFGE 7 PFGE DNA PFGE 16S rdna PFGE 1996 PulseNet CDC 8 PFGE CDC PFGE RAPD 3 AFLP 4 Ribotyping 5 RAPD Ribotyping 9 PFGE RAPD AFLP PFGE RAPD AFLP PFGE PulseNet 1990 PCR DNA 16S rdna DNA 1 16S rdna S rdna 16S rdna 1500

3 DNA 2000 DNA DNA 10 6 bp DNA PFGE DNA DNA DNA CD 10 DNA DNA 5 6 multi locus sequencing typing MLST PFGE 1990 ATGGT 16S rdnagyrb DNA global epidemiology

4 alleles PFGE A B C alleles A B A B 1 A C 10 A, B C MLST A,B,C 4. MLST PFGE Applied-Maths BioNumerics TM 1117 MLST MLST

5 MLST DNA clonal population nonclonal population S rdna clonal poplulation 18 nonclonal population 18 clonal population 1 nonclonoal population 1 A A nonclonal popultion 1 MLST 1 2 alleles nonclonal population multilocus sequence typing? Nonclonal population allele

6 MLST housekeeping 2 housekeeping 16S rdna 21 DNA gyrb housekeeping 24 housekeeping housekeeping gyr housekeeping

7 MLST 6, 25 housekeeping housekeeping Photobacterium damselae Pasteurella piscicida 16S rdna housekeeping housekeeping MLST housekeeping 6, 25 MLST housekeeping housekeeping PFGE MLST

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