CXC chemokine (ligand/receptor)

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Prosper Whitehead
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1 List of abbreviations -MEM alpha-modified minimal essential AKT protein kinase B (also abbreviated PKB) AMC 7-amino-4-methylcoumarin AP-1 activator protein 1 ARF ADP-ribosylation factor, a small GTP-binding protein ARP2/3 actin-related proteins2/3 ASAP1 the Arf-GTPase-activating protein 1 ATF activating transcription factor BSA bovin serum albumin CC (L/R) C chemokine (ligand/receptor) Cdc42 cell division cycle 42,a GTP binding protein CDK cyclin-dependent kinases Chk checkpoint kinases c-met mesenchymal-epithelial transition factor CXC(L/R) CXC chemokine (ligand/receptor) ECL enhanced chemiluminescence ECM extracellular matrix EGF(R) epidermal growth factor (receptor) EMT epithelial-mesenchymal transition ER estrogen receptor ERK extracellular signal-regulated kinase FA focal adhesion FACS fluorescence-activated cell sorting F-actin filament-actin FAK focal adhesion kinase FAT focal adhesion targeting domain FERM band 4.1, ezrin, radixin and moesin homology domain Fra-1(fosl-1) fos related antigen-1(fos like-1) FRAP fluorescence recovery after photobleaching GAP GTPase activating proteins GFP green fluorescent protein 147

2 GIT G-protein-coupled receptor kinase interacting protein Grb2 growth factor receptor-bound protein 2 GRAF GTPase activating protein for Rho associated with FAK GRK G-protein receptor kinase GST glutathione-s-transferase GTP guanosine Triphosphate HE haematoxylin and eosin HGF hepatocyte growth factor ILK integrin linked kinase I.P. Intraperitoneal injection JNK c-jun N-terminal kinase KD knock down KO knock out LD leucine-rich sequences LIMK LIM-domain kinase MAPK mitogen-activated protein kinase MDA microtubule disrupting agents MDM2 murine double minute 2 (mdm2 oncogene) MLC myosin light chain MLCK myosin light chain kinase MMTV mouse mammary tumor virus MMP matrix metalloproteinases NF-kB nuclear factor kappa-light-chain-enhancer of activated B cells N-WASP N-Wiskott Aldrich syndrome protein p130cas Crk-associated substrate PBS phosphate buffered saline PI propidium iodide PI3K phosphoinositide-3 kinase PKB protein kinases B PKL paxillin kinase linker PLC phospholipase C PPR proline-rich regions 148

3 PTEN phosphatase and tensin homolog PTP protein tyrosine phosphatase Rac Ras-related C3 botulinum toxin substrate 1 Ras Ras super family RhoA Ras homolog gene family, member A ROCK Rho-associated kinase qrt-pcr quantitative real time- polymerase chain reaction SDS-PAGE sodium dodecyl sulfate - poly acrylamide gel electroforese SF scatter factor SH Src-homology domain sirna short interference RNA shrna short hairpin RNA SRE serum response element PVDF polyvinylideenfluoride TAM tumor-associated macrophages TBP 0.05% Triton/0.5% BSA in PBS TIRF total internal reflection fluorescence TRE TPA response element 149

4 List of publications 1. Yafeng Ma, Bob van de Water and Sylvia Le Dévédec. Focal adhesion kinase inhibition as a potential strategy for anticancer therapies. (submitted to Drug of the Future) 2. Yafeng Ma, Saertje Verkoeijen, Maroesja J. van Nimwegen, John H. Meerman and Bob van de Water. Role of Fos related antigen-1 (Fra-1) in focal adhesion kinase (FAK) mediated chemoresistance in mammary adenocarcinoma tumors and lung metastases. Research paper (submitted to Molecular Pharmacology ) 3. Yafeng Ma, Saertje Verkoeijen and Bob van de Water. c-jun N-terminal kinase coordinates vincristine-induced Rho-kinase-dependent cell contractility through the focal adhesion-associated scaffold protein paxillin. Research paper (submitted to Journal Pharmacology Experimental Therapeutics) 4. Yafeng Ma, Hans de Bont, John H. Meerman and Bob van de Water. An autocrine CXCR3 activation-loop drives breast tumor cell migration and invasion through ERK and Akt signaling. Research paper (submitted to Molecular Cancer Therapeutics) 5. Yafeng Ma, Sylvia Le Dévédec, Saertje Verkoeijen and Bob van de Water. The Serine178 residue of paxillin determines EGF-induced cell migration of metastatic breast tumor cell MTLn3. (In preparation) 150

5 CURRICULUM VITAE Yafeng Ma was born on January 28 th, 1980 in HeNan, China. In 1997, she started her college education in Biochemistry department in Nanjing University (Nanjing, Jiang Su, China). In 2001, she received her bachelor degree and started her 3-year master study under supervision of Prof. Chen Junhui in Biochemistry and Molecular Biology (Biochemistry Dept., Life Science School) in the same university. Her research was about anti-tumor effects of extracts from human urine. From September 2004 till April 2009, she pursued her PhD project in Division of Toxicology in the Leiden/Amsterdam Center of Drug Research (Leiden University, The Netherlands) financed by a 4-year fellowship from the Chinese Scholar Council. Her research was under supervision of Prof. Bob van de Water and focused on focal adhesion-dependent signaling in breast cancer treatment. From May 2009, she is a postdoctoral researcher in the group of Prof. Laura Machesky at the Beatson Institute of Cancer Research in Glasgow, Scotland. 151

Cell Motility. Junfeng Ji ( 纪俊峰 ), PhD

Cell Motility. Junfeng Ji ( 纪俊峰 ), PhD Cell Motility Junfeng Ji ( 纪俊峰 ), PhD Professor Laboratory of Somatic Reprogramming and Stem Cell Aging Center for Stem Cell and Tissue Engineering School of Medicine Zhejiang University Email: jijunfeng@zju.edu.cnedu