March 2012 October 2013

Size: px
Start display at page:

Download "March 2012 October 2013"

Transcription

1 ACQUITY UPC 2 Peer Reviewed Journal Articles March 2012 October 2013 Analytical and Bioanalytical Chemistry Use, Analysis, and Regulation of Pesticides in Natural Extracts, Essential Oils, Concretes, and Absolutes June Analytical Methods Supercritical Fluid (carbon dioxide) Based Ultra Performance Convergence Chromatography for the Separation and Determination of Fulvestrant Diastereomers Angewandte Chemie Dienamine-Mediated Inverse-Electron-Demand Hetero-Diels Alder Reaction by Using an Enantioselective H-Bond-Directing Strategy December Chemistry, A European Journal Breaking Symmetry with Symmetry: Bifacial Selectivity in the Asymmetric Cycloaddition of Anthracene Derivatives Page 1 of 5

2 Chirality Improved Chiral SFC Screening for Analytical Method Development, August 2013 (online) European Respiratory Journal Application of Omics Technologies to Biomarker Discovery in Inflammatory Lung Diseases Food Chemistry Chemical Profiling of Triacylglycerols and Diacylglycerols in Cow Milk Fat by Ultra-performance Convergence Chromatography Combined with Quadrupole Time-of-Flight Mass Spectrometry Journal of the American Chemical Society Asymmetric Organocatalytic Thio-Diels Alder Reactions via Trienamine Catalysis March Journal of Chromatography A Development of Supercritical Fluid (Carbon Dioxide) Based Ultra Performance Convergence Chromatographis Stability Indicating Assay Method for the Determination of Clofarabine in Injection October 2013 (Online) Page 2 of 5

3 Determination of Adsorption Isotherms in Supercritical Fluid Chromatography, in press September 2013 (online) Strong Cation Exchange Chiral Stationary Phase A Comparative Study in High-Performance Liquid Chromatography and Subcritical Fluid Chromatography August 2013 (online) In-depth Characterization of Six Cellulose Tris-(3,5- dimethylphenylcarbamate) Chiral Stationary Phases in Supercritical Fluid Chromatography August Chromatographic Resolution of Closely Related Species: Separation of Warfarin and Hydroxylated Isomers The Evaluation of 25 Chiral Stationary Phases and the Utilization of Sub-2.0 μm Coated Polysaccharide Chiral Stationary Phases via Supercritical Fluid Chromatography Simultaneous and Rapid Analysis of Bile Acids Including Conjugates by Supercritical Fluid Chromatography Coupled to Tandem Mass Spectrometry Page 3 of 5

4 Strong Cation Exchange-Type Chiral Stationary Phase for Enantioseparation of Chiral Amines in Subcritical Fluid Chromatography Coupling Ultra High-Pressure Liquid Chromatography with Mass Spectrometry: Constraints and Possible Applications Evaluation and Comparison of Various Separation Techniques for the Analysis of Closely-Related Compounds of Pharmaceutical Interest March Analysis of Basic Compounds by Supercritical Fluid Chromatography: Attempts to Improve Peak Shape and Maintain Mass Spectrometry Compatibility November Comparison of Ultra-High Performance Supercritical Fluid Chromatography and Ultra-High Performance Liquid Chromatography November Page 4 of 5

5 Journal of Pharmaceutical and Biomedical Analysis Evaluation of Supercritical Fluid Chromatography for Testing of PEG Adducts in Pharmaceuticals September 2013 (online) Journal of Separation Science Efficient Optimization of Ultra-High Performance Supercritical Fluid Chromatographic Separation of Rosa Sericea by Response Surface Methodology Evaluation of Various Chromatographic Approaches for the Retention of Hydrophilic Compounds and MS Compatibility Molecular Microbiology The Candida Glabrata Sterol Scavenging Mechanism, Mediated by the ATP-Binding Cassette Transporter Aus1p, is Regulated by Iron Limitation April Page 5 of 5

[ APPLICATION NOTE ] The Separation of 8 -THC, 9 -THC, and Their Enantiomers by UPC 2 Using Trefoil Chiral Columns INTRODUCTION APPLICATION BENEFITS

[ APPLICATION NOTE ] The Separation of 8 -THC, 9 -THC, and Their Enantiomers by UPC 2 Using Trefoil Chiral Columns INTRODUCTION APPLICATION BENEFITS The Separation of 8 -THC, 9 -THC, and Their Enantiomers by UPC 2 Using Trefoil Chiral Columns Jacquelyn Runco, Andrew Aubin, and Catharine Layton Waters Corporation, Milford, MA, USA APPLICATION BENEFITS

More information

March 2012 December 2015

March 2012 December 2015 ACQUITY UPC 2 Journal Articles March 2012 December 2015 A total of 142 scientific journal articles on applications for Waters UPC 2 technology. Advanced Materials Research Analysis of Paclitaxel in Pharmaceutical

More information

Fast Separation of Triacylglycerols in Oils using UltraPerformance Convergence Chromatography (UPC 2 )

Fast Separation of Triacylglycerols in Oils using UltraPerformance Convergence Chromatography (UPC 2 ) Fast Separation of Triacylglycerols in Oils using UltraPerformance Convergence Chromatography (UPC 2 ) Mehdi Ashraf-Khorassani, 1 Larry T. Taylor, 1 Jinchuan Yang, 2 and Giorgis Isaac 2 1 Department of

More information

John P. McCauley and Rui Chen Waters Corporation, Milford, MA, USA INTRODUCTION APPLICATION BENEFITS WATERS SOLUTIONS KEY WORDS

John P. McCauley and Rui Chen Waters Corporation, Milford, MA, USA INTRODUCTION APPLICATION BENEFITS WATERS SOLUTIONS KEY WORDS Enantiomeric and diastereomeric separations of fragrance and essential oil components using the ACQUITY UPC 2 System with ACQUITY UPC 2 Trefoil Columns John P. McCauley and Rui Chen Waters Corporation,

More information

Lipid Class Separation Using UPC 2 /MS

Lipid Class Separation Using UPC 2 /MS Michael D. Jones, 1,3 Giorgis Isaac, 1 Giuseppe Astarita, 1 Andrew Aubin, 1 John Shockcor, 1 Vladimir Shulaev, 2 Cristina Legido-Quigley, 3 and Norman Smith 3 1 Waters Corporation, Milford, MA, USA 2 Department

More information

Determination of enantiomeric purity of Timolol Maleate by Supercritical Fluid Chromatography

Determination of enantiomeric purity of Timolol Maleate by Supercritical Fluid Chromatography Determination of enantiomeric purity of Timolol Maleate by Supercritical Fluid Chromatography Dr. Damian Connolly, (PMBRC, WIT) & Mr. Adrian Marley (Allergan Pharmaceuticals, Westport) PMBRC 3 rd Analytical

More information

Determination of Triglycerides and Waxes in Food Products Using Cool On-Column Injection and the MET- Biodiesel Capillary Column

Determination of Triglycerides and Waxes in Food Products Using Cool On-Column Injection and the MET- Biodiesel Capillary Column Page 1 of 6 Page 1 of 6 Return to Web Version Determination of Triglycerides and Waxes in Food Products Using Cool On-Column Injection and the MET- Biodiesel Capillary Column By: Michael D. Buchanan, Reporter

More information

Organic Chemistry Diversity of Carbon Compounds

Organic Chemistry Diversity of Carbon Compounds Organic Chemistry Diversity of Carbon Compounds Hydrocarbons The Alkanes The Alkenes The Alkynes Naming Hydrocarbons Cyclic Hydrocarbons Alkyl Groups Aromatic Hydrocarbons Naming Complex Hydrocarbons Chemical

More information

HICHROM. Chromatography Columns and Supplies NEW PRODUCTS. Catalogue 9. Hichrom Limited

HICHROM. Chromatography Columns and Supplies NEW PRODUCTS. Catalogue 9. Hichrom Limited HICHROM Chromatography Columns and Supplies NEW PRODUCTS Catalogue 9 1 The Markham Centre, Station Road Theale, Reading, Berks, RG7 4PE, UK Tel: +44 (0)118 930 3660 Fax: +44 (0)118 932 3484 Email: sales@hichrom.co.uk

More information

Journal of Chemical and Pharmaceutical Research, 2012, 4(11): Research Article

Journal of Chemical and Pharmaceutical Research, 2012, 4(11): Research Article Available online www.jocpr.com Journal of Chemical and Pharmaceutical Research, 2012, 4(11):4915-4920 Research Article ISSN : 0975-7384 CODEN(USA) : JCPRC5 Separation of the two enantiomers of Gatifloxacin

More information

Analytical and Preparative SFC Columns

Analytical and Preparative SFC Columns Analytical and Preparative SFC Columns Best Performance for Supercritical Fluid Chromatography Sepax SFC-Cyano... Sepax SFC-Amino... Sepax SFC-Pyridine... Sepax SFC-SCX... 5 Sepax SFC-Diol... 6 Sepax SFC-Silica...

More information

Analysis of the Non-Ionic Surfactant Triton-X Using UltraPerformance Convergence Chromatography (UPC 2 ) with MS and UV Detection

Analysis of the Non-Ionic Surfactant Triton-X Using UltraPerformance Convergence Chromatography (UPC 2 ) with MS and UV Detection Analysis of the Non-Ionic Surfactant Triton-X Using UltraPerformance Convergence Chromatography (UPC 2 ) with MS and UV Detection Jane Cooper, 1 Baiba Cabovska 2 1 Waters Corporation, Wilmslow, UK 2 Waters

More information

Improved Pirkie Type 1 Amide Type: Asymmetric carbon atoms are bonded directly with CONH group

Improved Pirkie Type 1 Amide Type: Asymmetric carbon atoms are bonded directly with CONH group 1 sur 6 26/06/2013 14:16 Special Merits of SUMICHIRAL OA 1.The large number of theoretical plates of the columns offer high resolution. 2.The packing materials have chemical stability and the columns have

More information

Lipid Analysis ISOLATION, SEPARATION, IDENTIFICATION AND. Bridgwater, England LIPIDOMIC ANALYSIS. Fourth Edition. Invergowrie, Dundee, Scotland

Lipid Analysis ISOLATION, SEPARATION, IDENTIFICATION AND. Bridgwater, England LIPIDOMIC ANALYSIS. Fourth Edition. Invergowrie, Dundee, Scotland Lipid Analysis ISOLATION, SEPARATION, IDENTIFICATION AND LIPIDOMIC ANALYSIS Fourth Edition WILLIAM W.CHRISTIE MRS Lipid Analysis Unit, Scottish Crop Research Institute, Dundee, Scotland Invergowrie, and

More information

HT5 - High Temperature Stationary Phase for Capillary Gas Chromatography

HT5 - High Temperature Stationary Phase for Capillary Gas Chromatography HT5 - High Temperature Stationary Phase for Capillary Gas Chromatography INTRODUCTION HT5 was the first carborane modified siloxane phase to be commercially available on fused silica capillary columns.

More information

Applications Summary Waters Corporation 1

Applications Summary Waters Corporation 1 Applications Summary 2012 Waters Corporation 1 UPC ² Key Messages UPC² is an exclusive, innovative separations tool which applies Waters proven UPLC technology to the traditional technique of SFC, broadening

More information

Sepax Technologies, Inc.

Sepax Technologies, Inc. Sepax Technologies, Inc. Sepax Technologies, Inc. develops and manufactures products in the area of chemical and biological separations, biosurfaces and proteomics. Sepax product portfolio includes ) liquid

More information

Andrea Perissi Specialista Applicativo, Waters Italia Waters Corporation 1

Andrea Perissi Specialista Applicativo, Waters Italia Waters Corporation 1 Interfacing UPC 2 to MS Andrea Perissi Specialista Applicativo, Waters Italia 2012 Waters Corporation 1 Agenda Introduction Traditional approaches to interfacing SFC to MS Ionization Mode Approach used

More information

Ultra Performance Liquid Chromatography Coupled to Orthogonal Quadrupole TOF MS(MS) for Metabolite Identification

Ultra Performance Liquid Chromatography Coupled to Orthogonal Quadrupole TOF MS(MS) for Metabolite Identification 22 SEPARATION SCIENCE REDEFINED MAY 2005 Ultra Performance Liquid Chromatography Coupled to Orthogonal Quadrupole TOF MS(MS) for Metabolite Identification In the drug discovery process the detection and

More information

CHEM 109A Organic Chemistry

CHEM 109A Organic Chemistry CEM 09A Organic Chemistry https://labs.chem.ucsb.edu/zakarian/armen/courses.html Chapter Isomers The arrangement of atoms in space MIDTERM Wednesday Feb. Chapter Sections.8.6 melting, boiling points, conformations

More information

Performance of an ultra low elution volume 96-well plate

Performance of an ultra low elution volume 96-well plate Performance of an ultra low elution volume 96-well plate Claude R. Mallet, Ziling Lu, Jeff R. Mazzeo, Uwe D. Neue Waters Corporation PittCon 2003 March 10-14 2003 Orlando, Florida Today s Challenges Faced

More information

Separation of Vitamin D and Vitamin D Metabolites on FLARE C18 MM (Mixed Mode) HPLC Column

Separation of Vitamin D and Vitamin D Metabolites on FLARE C18 MM (Mixed Mode) HPLC Column Diamond Analytics Technical Note: T131-1 Separation of Vitamin D and Vitamin D Metabolites on FLARE C1 MM (Mixed Mode) HPLC Column Introduction In this technical note, the versatility of the diamond-based

More information

Enhanced method development workflow for modern LC and SFC

Enhanced method development workflow for modern LC and SFC Enhanced method development workflow for modern LC and SFC Davy GUILLARME 18 th of September 2014 What can be done with modern LC? MONOLITHS HIGH TEMPERATURE 1 000 000 plates 12 m column 120 C.60 0.40

More information

Analysis of Alcohol and Alkylphenol Polyethers via Packed Column Supercritical Fluid Chromatography. Brian Jeffrey Hoffman

Analysis of Alcohol and Alkylphenol Polyethers via Packed Column Supercritical Fluid Chromatography. Brian Jeffrey Hoffman Analysis of Alcohol and Alkylphenol Polyethers via Packed Column Supercritical Fluid Chromatography by Brian Jeffrey Hoffman Dissertation submitted to the Graduate Faculty of Virginia Polytechnic Institute

More information

Small Molecule Science: Experimental designs for achieving ultra trace analysis

Small Molecule Science: Experimental designs for achieving ultra trace analysis Small Molecule Science: Experimental designs for achieving ultra trace analysis Michael P. Balogh Principal scientist Waters Corporation 211 Waters Corporation 1 www.cosmoscience.org The Society for Small

More information

Stereo-Isomers in Pesticides. Martin Rodler, CropLife International

Stereo-Isomers in Pesticides. Martin Rodler, CropLife International Stereo-Isomers in Pesticides Martin Rodler, CropLife International Outline Relevance of Stereo-Isomers for Pesticides Regulatory Evaluation of Pesticides Introduction to Stereo-Isomerism Enantiomers Diastereomers

More information

A Novel HILIC Column for High Speed N-linked Glycan Analysis

A Novel HILIC Column for High Speed N-linked Glycan Analysis A Novel HILIC for High Speed N-linked Glycan Analysis Application Note Biotherapeutics and Biosimilars Authors James Martosella, Oscar Potter, Danny Mancheno, and Jia Liu Agilent Technologies, Inc. Introduction

More information

Biochemistry. 2. Besides carbon, name 3 other elements that make up most organic compounds.

Biochemistry. 2. Besides carbon, name 3 other elements that make up most organic compounds. Biochemistry Carbon compounds Section 3-1 1. What is an organic compound? 2. Besides carbon, name 3 other elements that make up most organic compounds. 3. Carbon dioxide, CO 2, is NOT an organic compound.

More information

DETERMINATION OF COMPOSITION OF TRIACYLGLYCEROLS AND COMPOSITION AND CONTENT OF DI-ACYLGLYCEROLS BY CAPILLARY GAS CHROMATOGRAPHY, IN VEGETABLE OILS

DETERMINATION OF COMPOSITION OF TRIACYLGLYCEROLS AND COMPOSITION AND CONTENT OF DI-ACYLGLYCEROLS BY CAPILLARY GAS CHROMATOGRAPHY, IN VEGETABLE OILS INTERNATIONAL OLIVE COUNCIL COI/T.20/Doc. No 32 November 2013 ENGLISH Original: ENGLISH Príncipe de Vergara, 154 28002 Madrid España Telef.: +34 915 903 638 Fax: +34 915 631 263 - e-mail: iooc@internationaloliveoil.org

More information

Metabolomics: quantifying the phenotype

Metabolomics: quantifying the phenotype Metabolomics: quantifying the phenotype Metabolomics Promises Quantitative Phenotyping What can happen GENOME What appears to be happening Bioinformatics TRANSCRIPTOME What makes it happen PROTEOME Systems

More information

Analysis of Rosuvastatin in Dried Blood Spot and Plasma Using ACQUITY UPLC with 2D Technology

Analysis of Rosuvastatin in Dried Blood Spot and Plasma Using ACQUITY UPLC with 2D Technology Analysis of Rosuvastatin in Dried Blood Spot and Plasma Using ACQUITY UPLC with 2D Technology Claude Mallet, 1 Jennifer Simeone, 2 Paul Rainville 3 1 Workflow Integration Group, Separations Technologies,

More information

Immobilized Chiral Stationary Phases

Immobilized Chiral Stationary Phases Immobilized Chiral Stationary Phases Immobilization Silica Polymer Proprietary Immobilization Techniques CSP CIRALPAK CIRALPAK CIRALPAK CIRALPAK CIRALPAK CIRALPAK Immobilized Chiral Stationary Phases I-CSP

More information

Thermo Fisher Scientific, Sunnyvale, CA, USA; 2 Thermo Fisher Scientific, San Jose, CA, USA

Thermo Fisher Scientific, Sunnyvale, CA, USA; 2 Thermo Fisher Scientific, San Jose, CA, USA An Ultra High Resolution Glycan Column for Isomeric Separation and the Structural Identification of Labeled N-Glycans from Proteins Including Antibodies Udayanath Aich, 1 Julian Saba, 2 Rosa Viner, 2 Shanhua

More information

Lux. Five Distinct Polysaccharide Phases to Resolve 92 % of Your Enantiomers* Lux Cellulose-1. Lux Cellulose-3. Lux Cellulose-2.

Lux. Five Distinct Polysaccharide Phases to Resolve 92 % of Your Enantiomers* Lux Cellulose-1. Lux Cellulose-3. Lux Cellulose-2. Five Distinct Polysaccharide Phases to Resolve 9 % of Your Enantiomers* If Lux analytical columns ( 4.6 mm ID) do not provide at least an equivalent or better separation as compared to a competing column

More information

Ultra Columns. also available. ordering note

Ultra Columns. also available. ordering note : 3μm or 5μm particles; 100Å pore size Our broadest selection of stationary phases, including unique phases. High density bondings, for maximum retention. High-purity, Type B silica gives excellent peak

More information

Topic 3: Molecular Biology

Topic 3: Molecular Biology Topic 3: Molecular Biology 3.2 Carbohydrates and Lipids Essen=al Understanding: Carbon, hydrogen and oxygen are used to supply and store energy. Carbohydrates CARBOHYDRATES CHO sugars Primarily consist

More information

Metabolite identification in metabolomics: Database and interpretation of MSMS spectra

Metabolite identification in metabolomics: Database and interpretation of MSMS spectra Metabolite identification in metabolomics: Database and interpretation of MSMS spectra Jeevan K. Prasain, PhD Department of Pharmacology and Toxicology, UAB jprasain@uab.edu utline Introduction Putative

More information

3 Normal-Phase Columns. Reversed-Phase C18 Columns (ODS) Reversed-Phase Columns (Other than ODS) Columns for HTS and LC/MS Analysis.

3 Normal-Phase Columns. Reversed-Phase C18 Columns (ODS) Reversed-Phase Columns (Other than ODS) Columns for HTS and LC/MS Analysis. We are pleased to present the 11th YMC General Catalog. Since its formation in 1980, YMC Co., Ltd. has been working in the rapidly changing field of chemistry. Over this time, the rate of discovery of

More information

Macromolecules. 3. There are several levels of protein structure, the most complex of which is A) primary B) secondary C) tertiary D) quaternary

Macromolecules. 3. There are several levels of protein structure, the most complex of which is A) primary B) secondary C) tertiary D) quaternary Macromolecules 1. If you remove all of the functional groups from an organic molecule so that it has only carbon and hydrogen atoms, the molecule become a molecule. A) carbohydrate B) carbonyl C) carboxyl

More information

NEW! 200 m GC Columns for Detailed Analysis of cis/trans FAME Isomers

NEW! 200 m GC Columns for Detailed Analysis of cis/trans FAME Isomers Order: 00--00 (U.S.) -- (Global) NEW! 00 m GC Columns for Detailed Analysis of cis/trans FAME Isomers Leonard M. Sidisky, R&D Manager; and Michael D. Buchanan, Product Manager mike.buchanan@sial.com Over

More information

Carbohydrates and Lipids

Carbohydrates and Lipids Carbohydrates and Lipids Chapter 5: Macromolecules Macromolecules Smaller organic molecules join together to form larger molecules o macromolecules 4 major classes of macromolecules: o Carbohydrates o

More information

Core-Shell Technology for Proteins and Peptides

Core-Shell Technology for Proteins and Peptides Core-Shell Technology for Proteins and Peptides Better BioSeparations on HPLC and UHPLC Systems www.phenomenex.com/aeris Aeris Core-Shell Technology Core-Shell Particles Precision Engineered for Protein

More information

All living things are mostly composed of 4 elements: H, O, N, C honk Compounds are broken down into 2 general categories: Inorganic Compounds:

All living things are mostly composed of 4 elements: H, O, N, C honk Compounds are broken down into 2 general categories: Inorganic Compounds: Organic Chemistry All living things are mostly composed of 4 elements: H, O, N, C honk Compounds are broken down into 2 general categories: Inorganic Compounds: Do not contain carbon Organic compounds

More information

Measuring Lipid Composition LC-MS/MS

Measuring Lipid Composition LC-MS/MS Project: Measuring Lipid Composition LC-MS/MS Verification of expected lipid composition in nanomedical controlled release systems by liquid chromatography tandem mass spectrometry AUTHORED BY: DATE: Sven

More information

Available online at Scholars Research Library

Available online at  Scholars Research Library Available online at www.scholarsresearchlibrary.com Scholars Research Library Der Pharmacia Lettre, 2015, 7 (3):157-161 (http://scholarsresearchlibrary.com/archive.html) ISSN 0975-5071 USA CODEN: DPLEB4

More information

Type Analysis of Citrus Essential Oils by Multidimensional Supercritical Fluid Chromatography/ Gas Chromatography

Type Analysis of Citrus Essential Oils by Multidimensional Supercritical Fluid Chromatography/ Gas Chromatography 25 Type Analysis of Citrus Essential Oils by Multidimensional Supercritical Fluid Chromatography/ Gas Chromatography Takashi YARITA, Akira NoMURA and Yoshiyuki HoRIMOTo Department of Analytical Chemistry,

More information

Enantiomeric and Diastereoisomeric Resolutions of Chiral Triazole Fungicides using Lux Polysaccharide-Based Chiral Stationary Phases

Enantiomeric and Diastereoisomeric Resolutions of Chiral Triazole Fungicides using Lux Polysaccharide-Based Chiral Stationary Phases T-1164 APPLICATIS Enantiomeric and Diastereoisomeric Resolutions of Chiral Triazole Fungicides using Lux Polysaccharide-Based Chiral Stationary Phases Marc Jacob, Liming Peng, Michael Klein, Tom eveland,

More information

Separation of Macrocyclic Lactones (Avermectins) on FLARE C18 MM & FLARE C18+ Columns

Separation of Macrocyclic Lactones (Avermectins) on FLARE C18 MM & FLARE C18+ Columns Separation of Macrocyclic Lactones (Avermectins) on FLARE C8 MM & FLARE C8+ Columns Introduction Diamond Analytics Technical Note: T05- Avermectins are a series of 6-membered macrocyclic lactone derivatives

More information

LC/MS Method for Comprehensive Analysis of Plasma Lipids

LC/MS Method for Comprehensive Analysis of Plasma Lipids Application Note omics LC/MS Method for Comprehensive Analysis of Plasma s Authors Tomas Cajka and Oliver Fiehn West Coast Metabolomics Center, University of California Davis, 451 Health Sciences Drive,

More information

UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes

UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes UPLC/MS Monitoring of Water-Soluble Vitamin Bs in Cell Culture Media in Minutes Catalin E. Doneanu, Weibin Chen, and Jeffrey R. Mazzeo Waters Corporation, Milford, MA, U.S. A P P L I C AT ION B E N E F

More information

Natural Product Preparative HPLC Purification from Complex Crude Extraction Mixtures by Granular Bonded and Unbonded Silica Gel

Natural Product Preparative HPLC Purification from Complex Crude Extraction Mixtures by Granular Bonded and Unbonded Silica Gel Natural Product Preparative PLC Purification from Complex Crude Extraction Mixtures by Granular Bonded and Unbonded Silica Gel Lori Sandford Paul Garms Theresa Riley Jochen Saar Grace Davison Discovery

More information

LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES. Gilles Frache Materials Characterization Day October 14 th 2016

LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES. Gilles Frache Materials Characterization Day October 14 th 2016 LOCALISATION, IDENTIFICATION AND SEPARATION OF MOLECULES Gilles Frache Materials Characterization Day October 14 th 2016 1 MOLECULAR ANALYSES Which focus? LOCALIZATION of molecules by Mass Spectrometry

More information

Chapter Three (Biochemistry)

Chapter Three (Biochemistry) Chapter Three (Biochemistry) 1 SECTION ONE: CARBON COMPOUNDS CARBON BONDING All compounds can be classified in two broad categories: organic compounds and inorganic compounds. Organic compounds are made

More information

Determination of glycerol derivatives by High-performance liquid chromatography

Determination of glycerol derivatives by High-performance liquid chromatography Determination of glycerol derivatives by High-performance liquid chromatography JUAN CARLOS BELTRÁN PRIETO a, JIŘÍ PECHA a, VĚRA KAŠPÁRKOVÁ b,c, KAREL KOLOMAZNÍK a a Department of Automation and Control

More information

Edgar Naegele. Abstract

Edgar Naegele. Abstract Simultaneous determination of metabolic stability and identification of buspirone metabolites using multiple column fast LC/TOF mass spectrometry Application ote Edgar aegele Abstract A recent trend in

More information

Ultra Columns HPLC COLUMNS

Ultra Columns HPLC COLUMNS Acetaminophen, Narcotic Analgesics.............519 Aldehydes, Ketones...........................500 Beclomethasone.............................523 Corticosteroids..............................529 Explosives..................................501

More information

Lipid Analysis. Andréina Laffargue, IRD CRYMCEPT Montpellier workshop, October 17th Introduction to lipid structures

Lipid Analysis. Andréina Laffargue, IRD CRYMCEPT Montpellier workshop, October 17th Introduction to lipid structures Lipid Analysis Andréina Laffargue, IRD CRYMCEPT Montpellier workshop, October 17th 2005 Introduction to lipid structures Fatty acids Acylglycerols Glycerophospholipids Sterols Strategies involved in lipid

More information

GlycanPac AXR-1 Columns

GlycanPac AXR-1 Columns CHRMATGRAPHY GlycanPac AXR- Columns For High Resolution Glycan Analysis Product Specifications The Thermo Scientific GlycanPac AXR- columns are highperformance, silica-based HPLC columns for simultaneous

More information

Removal of Triton X-100 from Plasma Samples Using Mixed-Mode Solid Phase Extraction (SPE)

Removal of Triton X-100 from Plasma Samples Using Mixed-Mode Solid Phase Extraction (SPE) Removal of Triton X- from Plasma Samples Using Mixed-Mode Solid Phase Extraction (SPE) Jonathan P. Danaceau, Erin Chambers, and Kenneth J. Fountain Waters Corporation, 34 Maple Street, Milford, MA USA

More information

Non-GLP Bioanalysis. Introduction. Lab Space and Instrumentation

Non-GLP Bioanalysis. Introduction. Lab Space and Instrumentation Non-GLP Bioanalysis Introduction Founded in 2006, WuXi AppTec s non-glp bioanalytical service has established a state-of-the-art LC-MS/MS platform for bioanalysis in the drug discovery field. Over the

More information

Rapid Quantitative Analysis of Cannabidiol from Consumer Products Using UltraPerformance Convergence Chromatography

Rapid Quantitative Analysis of Cannabidiol from Consumer Products Using UltraPerformance Convergence Chromatography Rapid Quantitative Analysis of Cannabidiol from Consumer Products Using UltraPerformance Convergence Chromatography Andrew Aubin Waters Corporation, Milford, MA, USA APPLICATION BENEFITS Direct injection

More information

Identification of Imidacloprid Metabolites in Onions Using High Resolution Accurate Mass Spectrometry (LC/Q-TOF MS) and Accurate Mass Tools

Identification of Imidacloprid Metabolites in Onions Using High Resolution Accurate Mass Spectrometry (LC/Q-TOF MS) and Accurate Mass Tools Identification of Imidacloprid Metabolites in Onions Using High Resolution Accurate Mass Spectrometry (LC/Q-TOF MS) and Accurate Mass Tools Application ote Food Authors E. Michael Thurman and Imma Ferrer

More information

Molecules 2014, 19, ; doi: /molecules Article

Molecules 2014, 19, ; doi: /molecules Article Molecules 2014, 19, 342-351; doi:10.3390/molecules19010342 Article OPEN ACCESS molecules ISSN 1420-3049 www.mdpi.com/journal/molecules Development of a Supercritical Fluid Chromatography-Tandem Mass Spectrometry

More information

SEPARATION OF ENANTIOMERS BY CHIRALLY MODIFIED MEMBRANES

SEPARATION OF ENANTIOMERS BY CHIRALLY MODIFIED MEMBRANES XXIII ARS SEPARATORIA Toruń, Poland 2008 41 SEPARATION OF ENANTIOMERS BY CHIRALLY MODIFIED MEMBRANES Izabela KOTER Nicolaus Copernicus University, Faculty of Chemistry, 7 Gagarin St., 87-100 Torun, Poland

More information

Mass Spectrometry Introduction

Mass Spectrometry Introduction Mass Spectrometry Introduction Chem 744 Spring 2013 What MS is and is not MS is NOT a spectroscopic method. Molecules are not absorbing EM radiation MS is the generation, separation and characterization

More information

Probing for Packaging Migrants in a Pharmaceutical Impurities Assay Using UHPLC with UV and Mass Detection INTRODUCTION

Probing for Packaging Migrants in a Pharmaceutical Impurities Assay Using UHPLC with UV and Mass Detection INTRODUCTION Probing for Packaging Migrants in a Pharmaceutical Impurities Assay Using UHPLC with UV and Mass Detection Michael Jones Waters Corporation, Wilmslow, UK APPLICATION BENEFITS The ACQUITY Arc System is

More information

Chromatography Vacuum Ultraviolet Spectroscopy

Chromatography Vacuum Ultraviolet Spectroscopy Application Note Differentiation and Determination Differentiation and Determination of Fatty Acid Methyl of Fatty Esters Acid by Gas Methyl Chromatography Esters by Vacuum Gas Ultraviolet Spectroscopy

More information

Studies on stereoselective separations of the azole antifungal drugs ketoconazole and itraconazole using HPLC and SFC on silica-based polysaccharides

Studies on stereoselective separations of the azole antifungal drugs ketoconazole and itraconazole using HPLC and SFC on silica-based polysaccharides SFC Dossier Studies on stereoselective separations of the azole antifungal drugs ketoconazole and itraconazole using HPLC and SFC on silica-based polysaccharides A. Thienpont 1, J. Gal 2, C. Aeschlimann

More information

Application Note. Authors: C. Ledesma, M. Gibert, J.R. Gibert Ingenieria Analitica S.L. Extracts from various food products

Application Note. Authors: C. Ledesma, M. Gibert, J.R. Gibert Ingenieria Analitica S.L. Extracts from various food products High-Performance Anion-Exchange Chromatography coupled with Pulsed Electrochemical Detection as a powerful tool to evaluate lactose content in lactose-free labeled products Application Note Authors: C.

More information

LC Columns - Exceed the limit. A premium inert range of LC columns delivering optimal peak shape. ProteCol -P PEEK lined

LC Columns - Exceed the limit. A premium inert range of LC columns delivering optimal peak shape. ProteCol -P PEEK lined ProteCol LC Columns - Exceed the limit A premium inert range of LC columns delivering optimal peak shape. ProteCol -G Glass lined ProteCol -P PEEK lined B C A D E F A Column end cap B PEEK frit housing

More information

Ultra High Definition Optimizing all Analytical Dimensions

Ultra High Definition Optimizing all Analytical Dimensions Ultra High Definition Optimizing all Analytical Dimensions Sensitivity Dynamic Range Signal Response Linearity Separation Speed Peak Capacity Chromatogram Mass Spectrum Mass Accuracy Resolving Power Acquisition

More information

Organic Molecules. Contain C

Organic Molecules. Contain C Contain C Organic Molecules Can form 4 strong covalent bonds Ergo can form many complex, stable molecules Chemistry of life is complex, and requires complex molecules However, several kinds of molecules

More information

January 31, Chemistry of Life. Carbohydrates. Lipids. Proteins. Biologically Important Macromolecules. Nucleic Acids

January 31, Chemistry of Life. Carbohydrates. Lipids. Proteins. Biologically Important Macromolecules. Nucleic Acids Chemistry of Life Carbohydrates Lipids Proteins Biologically Important Macromolecules Nucleic Acids Polymers Polymers are large molecules of repeating sub units (building blocks) Individual Building Blocks......can

More information

Gas Chromatography/ Mass Spectrometry

Gas Chromatography/ Mass Spectrometry Gas Chromatography/ Mass Spectrometry Edited by H.F. Linskens and J.F. Jackson Contributors R.S. Bandurski G. Combaut A.Ehmann P.Hedden B.Janistyn H.Kameoka H.Kodama D.V.Lynch J.K.MacLeod H.Nyberg L.M.S.Palni

More information

Analysis of L- and D-Amino Acids Using UPLC Yuta Mutaguchi 1 and Toshihisa Ohshima 2*

Analysis of L- and D-Amino Acids Using UPLC Yuta Mutaguchi 1 and Toshihisa Ohshima 2* Analysis of L- and D-Amino Acids Using UPLC Yuta Mutaguchi 1 and Toshihisa Ohshima 2* 1 Department of Biotechnology, Akita Prefectural University, Akita City, Japan; 2 Department of Biomedical Engineering,

More information

Fortis Method Development Options

Fortis Method Development Options Method Dev Fortis Method Development Options Choice of Stationary phase functionality Based on Ultra pure silica Reversed Phase (RP) and Normal Phase (NP) options Fortis Ho - Polar endcapped C8 - Increased

More information

Impact of Reversed-Phase Chiral Chromatography on the LC-MS Analysis of Drugs in Biological Fluids

Impact of Reversed-Phase Chiral Chromatography on the LC-MS Analysis of Drugs in Biological Fluids Impact of Reversed-Phase Chiral Chromatography on the LC-MS Analysis of Drugs in Biological Fluids David S. Bell, Carmen T. Santasania, Jennifer Claus, Wayne K. Way, and Craig R. Aurand Supelco, Div. of

More information

ASSAY AND IMPURITY METHOD FOR DURACOR TABLETS BY HPLC

ASSAY AND IMPURITY METHOD FOR DURACOR TABLETS BY HPLC ASSAY AND IMPURITY METHOD FOR DURACOR TABLETS BY HPLC METHOD APPROVALS Norvin Pharma Inc. Author Analytical Laboratory Approver Analytical Laboratory Group Leader Approver Manager Quality Control Chemistry

More information

Organic Chemistry Laboratory Fall Lecture 3 Gas Chromatography and Mass Spectrometry June

Organic Chemistry Laboratory Fall Lecture 3 Gas Chromatography and Mass Spectrometry June 344 Organic Chemistry Laboratory Fall 2013 Lecture 3 Gas Chromatography and Mass Spectrometry June 19 2013 Chromatography Chromatography separation of a mixture into individual components Paper, Column,

More information

Analysis of glipizide binding to normal and glycated human serum Albumin by high-performance affinity chromatography

Analysis of glipizide binding to normal and glycated human serum Albumin by high-performance affinity chromatography Analytical and Bioanalytical Chemistry Electronic Supplementary Material Analysis of glipizide binding to normal and glycated human serum Albumin by high-performance affinity chromatography Ryan Matsuda,

More information

Glycerolipid Analysis. LC/MS/MS Analytical Services

Glycerolipid Analysis. LC/MS/MS Analytical Services Glycerolipid Analysis LC/MS/MS Analytical Services Molecular Characterization and Quantitation of Glycerophospholipids in Commercial Lecithins by High Performance Liquid Chromatography with Mass Spectrometric

More information

Metabolite identification in metabolomics: Metlin Database and interpretation of MSMS spectra

Metabolite identification in metabolomics: Metlin Database and interpretation of MSMS spectra Metabolite identification in metabolomics: Metlin Database and interpretation of MSMS spectra Jeevan K. Prasain, PhD Department of Pharmacology and Toxicology, UAB jprasain@uab.edu Outline Introduction

More information

Macromolecules. The four groups of biomolecules or macromolecules found in living things which are essential to life are: 1. PROTEINS 1.

Macromolecules. The four groups of biomolecules or macromolecules found in living things which are essential to life are: 1. PROTEINS 1. Macromolecules The four groups of biomolecules or macromolecules found in living things which are essential to life are: 1. PROTEINS 1. CARBOHYDRATES 1. LIPIDS 1. NUCLEIC ACIDS Carbon Compounds All compounds

More information

A Definitive Lipidomics Workflow for Human Plasma Utilizing Off-line Enrichment and Class Specific Separation of Phospholipids

A Definitive Lipidomics Workflow for Human Plasma Utilizing Off-line Enrichment and Class Specific Separation of Phospholipids A Definitive Lipidomics Workflow for Human Plasma Utilizing Off-line Enrichment and Class Specific Separation of Phospholipids Jeremy Netto, 1 Stephen Wong, 1 Federico Torta, 2 Pradeep Narayanaswamy, 2

More information

[ APPLICATION NOTE ] Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector

[ APPLICATION NOTE ] Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector Profiling Mono and Disaccharides in Milk and Infant Formula Using the ACQUITY Arc System and ACQUITY QDa Detector Mark Benvenuti, Gareth Cleland, and Jennifer Burgess Waters Corporation, Milford, MA, USA

More information

Chemistry 107 Exam 3 Study Guide

Chemistry 107 Exam 3 Study Guide Chapter 7 Chemistry 107 Exam 3 Study Guide 7.1 Recognize the aldehyde, ketone and hydroxyl (-OH) functional groups found in carbohydrates. Differentiate between mono-, di-, and polysaccharides. Master

More information

Biology: Life on Earth Chapter 3 Molecules of life

Biology: Life on Earth Chapter 3 Molecules of life Biology: Life on Earth Chapter 3 Molecules of life Chapter 3 Outline 3.1 Why Is Carbon So Important in Biological Molecules? p. 38 3.2 How Are Organic Molecules Synthesized? p. 38 3.3 What Are Carbohydrates?

More information

Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by HPLC and LC-MS *

Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by HPLC and LC-MS * 74 Journal of Zhejiang University SIEE ISS 19-395 http://www.zju.edu.cn/jzus E-mail: jzus@zju.edu.cn Separation and identification of cis and trans isomers of 2-butene-1,4-diol and lafutidine by PL and

More information

CHM 424L Organic Laboratory, Dr. Laurie S. Starkey Introduction to Mass Spectrometry

CHM 424L Organic Laboratory, Dr. Laurie S. Starkey Introduction to Mass Spectrometry CM 424L rganic Laboratory, Dr. Laurie S. Starkey Introduction to Mass Spectrometry Mass spectrometry is used to determine a sample's molecular mass and molecular formula. Some structural information can

More information

Chem 60 Takehome Test 2 Student Section

Chem 60 Takehome Test 2 Student Section Multiple choice: 1 point each. Mark only one answer for each question. 1. are composed primarily of carbon and hydrogen, but may also include oxygen, nitrogen, sulfur, phosphorus, and a few other elements.

More information

The high efficiency of sub-2 µm UHPLC columns combined with the low pressure and high speed of monolith columns.

The high efficiency of sub-2 µm UHPLC columns combined with the low pressure and high speed of monolith columns. The high efficiency of sub-2 µm UHPLC columns combined with the low pressure and high speed of monolith columns. Fast High peak capacity Low back pressure PROTEIN PEPTIDE UHPLC COLUMNS Put these new, high-performance

More information

COSMOSIL CHiRAL Series

COSMOSIL CHiRAL Series Immobilized Polysaccharide Derivative-Based Chiral Columns CSMSIL CiRAL Series Immobilized chiral selectors can withstand many different solvents Sharpen peaks with 3 μm particles Preparative separations

More information

Analysis of Omega 3 and Omega 6 FAMEs in Fish Oil and Animal Fat Using an Agilent J&W DB-FATWAX Ultra Inert GC Column

Analysis of Omega 3 and Omega 6 FAMEs in Fish Oil and Animal Fat Using an Agilent J&W DB-FATWAX Ultra Inert GC Column Application Note Food Analysis of Omega 3 and Omega 6 FAMEs in Fish Oil and Animal Fat Using an Agilent J&W DB-FATWAX Ultra Inert GC Column Authors Ingrid Van Der Meer, Yun Zou, and Gustavo Serrano Agilent

More information

[APPLICATION NOTE] DETERMINATION OF AMINO ACIDS IN BEERS USING THE UPLC AMINO ACID ANALYSIS SOLUTION

[APPLICATION NOTE] DETERMINATION OF AMINO ACIDS IN BEERS USING THE UPLC AMINO ACID ANALYSIS SOLUTION DETERMINATION OF AMINO ACIDS IN BEERS USING THE UPLC AMINO ACID ANALYSIS SOLUTION Mark E. Benvenuti and Paula Hong Waters Corporation, Milford, MA USA INTRODUCTION: Beer is a complex matrix consisting

More information

Capillary electromigration techniques:

Capillary electromigration techniques: CE capillary electrophoresis CZE capillary zone electrophoresis EKC electrokinetic chromatography MEKC/MECC micellar electrokinetic chromatography MEEKC microemulsion electrokinetic chromatography CEC

More information

A Fully Integrated Workflow for LC-MS/MS Analysis of Labeled and Native N-Linked Glycans Released From Proteins

A Fully Integrated Workflow for LC-MS/MS Analysis of Labeled and Native N-Linked Glycans Released From Proteins A Fully Integrated Workflow for LC-MS/MS Analysis of Labeled and Native N-Linked Glycans Released From Proteins Udayanath Aich, 1 Julian Saba, 2 Xiaodong Liu, 1 Srinivasa Rao, 1 Yury Agroskin, 1 and Chris

More information

Chapter 1. Chemistry of Life - Advanced TABLE 1.2: title

Chapter 1. Chemistry of Life - Advanced TABLE 1.2: title Condensation and Hydrolysis Condensation reactions are the chemical processes by which large organic compounds are synthesized from their monomeric units. Hydrolysis reactions are the reverse process.

More information

Chapter 3. Table of Contents. Section 1 Carbon Compounds. Section 2 Molecules of Life. Biochemistry

Chapter 3. Table of Contents. Section 1 Carbon Compounds. Section 2 Molecules of Life. Biochemistry Biochemistry Table of Contents Section 1 Carbon Compounds Section 2 Molecules of Life Section 1 Carbon Compounds Objectives Distinguish between organic and inorganic compounds. Explain the importance of

More information

Chemistry 1120 Exam 2 Study Guide

Chemistry 1120 Exam 2 Study Guide Chemistry 1120 Exam 2 Study Guide Chapter 6 6.1 Know amines are derivatives of ammonia, which is not an amine. Classify amines as primary, secondary or tertiary. Master Tutor Section 6.1 Review Section

More information

Introduction. and aryl-α-amino ketones, 11

Introduction. and aryl-α-amino ketones, 11 Enantiomeric Resolution of γ-amino Acids Bull. Korean Chem. Soc. 2011, Vol. 32, No. 8 3017 DOI 10.5012/bkcs.2011.32.8.3017 Liquid Chromatographic Resolution of Vigabatrin and Its Analogue γ-amino Acids

More information