Immobilized Chiral Stationary Phases

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2 Immobilized Chiral Stationary Phases

3 Immobilization Silica Polymer Proprietary Immobilization Techniques CSP CIRALPAK CIRALPAK CIRALPAK CIRALPAK CIRALPAK CIRALPAK

4 Immobilized Chiral Stationary Phases I-CSP Selector Structure Amylose 3,5-dimethylphenylcarbamate R= C 3 C 3 AD Cellulose 3,5-dimethylphenylcarbamate R= C 3 C 3 D - Cellulose - 3,5-dichlorophenylcarbamate R= Unique Selector Amylose 3-chlorophenylcarbamate R= Unique Selector - Amylose - 3,5-dichlorophenylcarbamate Unique Selector Amylose 3-chloro-4-methylphenylcarbamate R= C 3 AZ

5 Why Immobilized Phases? Stable to all normal phase, polar & reversed phase solvents just like the achiral PLC columns you use Solvent flexibility enhances the probability of success For preparative separations the wide range of compatible solvents allows best sample solubility ew Chiral Selectors previously not accessible, due to their solubility in the mobile phase, are now possible (CIRALPAK / and )

6 Polysaccharide Chiral Stationary Phases - Coated Selectors - Memory effects Solvent immiscibility Analyte solubility Care has to be taken to regenerate coated selectors Solvent immiscibility may cause column inlet clogged causing high pressure developed Solvent selection restricted

7 Immobilized Stationary Phases Attributes Coated Selectors Immobilized Selectors Reliability Daicel authentic selectors Daicel authentic selectors Robustness Restricted solvent selection Solvent flexibility Reproducibility Affected by possible memory effects Easy column regeneration Loading capacity igh loading capacity igh loading capacity greatly improved due to analyte solubility

8 Innovative Column Technology

9 Complementarity CIRALPAK Meclizine Column size Mobile phase Flow rate Temperature Detection 0.46mm x 250mm long n-exane/ipa/dea=95/5/ ml/min. 25 UV 254nm Retention Time [min] Retention Time [min] 30 Column size Mobile phase Flow rate Temperature Detection γ-phenyl-γ-butyrolactone 0.46mm x 250mm long n-exane/et=90/10 1.0ml/min. 25 UV 220nm

10 Complementarity CIRALPAK & S 2 Indapamide Column size Mobile phase Flow rate Temperature Detection 0.46mm x 250mm long n-exane/et/dea=50/50/ ml/min. 25 UV 254nm Retention Time [min] Retention Time [min] 30 Column size Mobile phase Flow rate Temperature Detection 5-Benzylhydantoin 0.46mm x 250mm long n-exane/ipa=80/20 1.0ml/min. 25 UV 220nm

11 Complementarity CIRALPAK Column size Mobile phase Flow rate Temperature Detection Retention Time [min] 25 CEt 0 Amlodipine MeC mm x 250mm long n-hexane/ipa/mea*=80/20/ ml/min. 40 UV 254nm *MEA=2-aminoethanol 2 Cisapride F Retention Time [min] 20 Column size Mobile phase Flow rate Temperature Detection 0.46mm x 250mm long n-exane/ipa/dea=50/50/ ml/min. 25 UV 254nm

12 Complementarity CIRALPAK Cetirizine Retention Time [min] 30 Column size Mobile phase Flow rate Temperature Detection 0.46mm x 250mm long n-exane/et/ac/dea=50/50/0.1/ ml/min. 25 UV 230nm

13 Complementarity CIRALPAK Methyclothiazide 2 S S Retention Time [min] 20 Column size Mobile phase Flow rate Temperature Detection 0.46mm x 250mm long n-exane/et/dea=50/50/ ml/min. 25 UV 230nm

14 Complete Product Portfolio Polysaccharides: CIRALPAK and CIRALCEL ormal and Reversed Phases Immobilized polysaccharides:,,,,, Coated polysaccharides: AD, AS, AY, AZ B, C, D, F, G, J, K, Z 3-µm polysaccharides:,,,,, Specialty Selectors AD, AS, AY, AZ, D, J, Z, X Protein-based Phases: CIRALPAK AGP, CB and SA, BSA, DSA, MSA and RSA Ligand Exchange: CIRALPAK MA+, CIRALPAK W Chiral Crown Ethers: CRWPAK CR+, CRWPAK CR- Lindner Anion Exchange Phases: CIRALPAK QD-AX, CIRALPAK Q-AX Zwitterionic Stationary Phases: CIRALPAK ZWIX(+), CIRALPAK ZWIX(-)

15 Global Reach

16 Chiral Technologies Worldwide Strasbourg, France West Chester, PA Tokyo, Japan yderabad, India Shanghai, China