OIE Reference Laboratory Reports Activities
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1 OIE Reference Laboratory Reports Activities Activities in 2013 This report has been submitted : :34:26 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Newcastle disease Address of laboratory: OIE Reference Laboratory for Newcastle disease Istituto Zooprofilattico Sperimentale delle Venezie (IZSVe), Viale dell Università Legnaro (PD) - Italy Tel.: Fax: address: icapua@izsvenezie.it website: Name (including Title) of Head of Laboratory (Responsible Official): Giovanni Cattoli Director of the Research and Innovation Department Istituto Zooprofilattico Sperimentale delle Venezie Viale dell Università Legnaro- Padova, Italy Name (including Title and Position) of OIE Reference Expert: Ilaria Capua Director Division of Comparative Biomedical Sciences OIE Reference laboratory for Newcastle Disease and Avian Influenza FAO Refence Centre for Animal Influenza and Newcastle Disease Istituto Zooprofilattico Sperimentale delle Venezie Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,
2 ToR: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic s for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency ing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of performed last year Indirect diagnostic s Nationally Internationally Haemoagglutination inhibition () yes Direct diagnostic s Nationally Internationally Virus isolation yes Real-time RT-PCR yes RT-PCR yes Sequencing of NDV genes yes ToR: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2013
3 Type of reagent available Related diagnostic Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients positive antigens 3732 (ml) 126 (ml) 1 positive antigens 3732 (ml) 104 (ml) 17 positive sera 0 (ml) 57 (ml) 1 positive sera 0 (ml) 128 (ml) negative serum 160 (ml) 77 (ml) 1 negative serum 160 (ml) 105 (ml) Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? OIE Reference Laboratory Reports Activities,
4 ToR: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? Name of the new or diagnostic method or vaccine developed Description and References (Publication, website, etc.) Pathotyping of Newcastle Disease Virus by pyrosequencing De Battisti, C., Salomoni, A., Ormelli, S., Monne, I., Capua, I., and Cattoli, G. Rapid pathotyping of Newcastle Disease Virus by pyrosequencing. J. Virol. Methods 188: ToR: To provide diagnostic ing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic ing for other OIE Member Countries? Name of OIE Member Country seeking assistance Date (month). samples received for provision of diagnostic support. samples received for provision of confirmatory diagnoses MOROCCO April 2 LIBYA April 18 JORDAN May 36 EGYPT May 204 JORDAN June 4 SAUDI ARABIA June 34 SAUDI ARABIA July 42 JORDAN August 2 EGYPT September 49 BANGLADESH September 6 COSTA RICA October 15 LIBYA December 5 4 OIE Reference Laboratory Reports Activities, 2013
5 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided AZERBAIJAN Laboratory assessment with special focus on HPAI and ND diagnostic capacity In loco and remote assistance IRAN OIE Twinning In loco and remote assistance EGYPT Laboratory diagnosis for avian viruses including ND In loco and remote assistance ToR: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? Title of the study Duration Purpose of the study Partners (Institutions) EMIDA ERA-Net Epi- Seq 3 years Molecular epidemiology of epizootic diseases using next generation sequencing technology CODA-CERVA, Friedrich-Loeffle- -Institut, Swedish University of Agricultural Sciences, Institute for Animal Health, Istituto Zooprofilattico Sperimentale delle Venezie, University of Glasgow OIE Twinning 2 years Implementation of capacity building of the CVL for the early detection and diagnosis of AI and ND and other major viral diseases of poultry IZSVe, CVL-IVO ANIHWA - NADIV 3 years Cooperation and coordination of National Research Programmes on Animal Health and Welfare of farm animals, including fish and bees IZSVe, Kimron Vet. Insititute Israel Characterization of ND strains in Ethiopia 1 year Molecular epidemiology of Newcastle Disease Faculty of Veterinary Medicine- University of Gondar, Ethiopia;IZSVe OIE Reference Laboratory Reports Activities,
6 ToR: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: 2 1.De Battisti, C., Salomoni, A., Ormelli, S., Monne, I., Capua, I., and Cattoli, G. Rapid pathotyping of Newcastle Disease Virus by pyrosequencing. J. Virol. Methods 188: Terregino, C., Aldous, E. W., Heidari, A., Fuller, C. M., De Nardi, R., Manvell, R. J., Beato, M. S., Shell, W. M., Monne, I., Brown, I. H., Alexander, D. J., and Capua, I. Antigenic and genetic analyses of isolate APMV/wigeon/Italy/3920-1/2005 indicate that it represents a new avian paramyxovirus (APMV-12). Arch. Virol. 158: b) International conferences: 0 c) National conferences: 0 d) Other: (Provide website address or link to appropriate information) wcastle&catid=51&itemid=201 ToR: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative s or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: 0 b) Seminars: 0 c) Hands-on training courses: 0 d) Internships (>1 month): 2 6 OIE Reference Laboratory Reports Activities, 2013
7 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country d Turkey 1 d Ethiopia 1 ToR: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO Is your laboratory accredited by an international accreditation body? Test for which your laboratory is accredited Virus isolation in embryonated eggs Accreditation body ACCREDIA Italian Accreditation System ACCREDIA Italian Accreditation System 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2012, Chapter or Manual of Diagnostic Tests for Aquatic Animals 2012, Chapter 1.1.1) ToR: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? OIE Reference Laboratory Reports Activities,
8 ToR: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency ing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proficiency s? Purpose of the proficiency s: 1 Role of your Reference Laboratory (organiser/ participant). participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. OFFLU Proficiency Test on AI and ND Participants Information available from organiser (APS.USDA) Information available from organiser (APS.USDA) an Proficiency Test on AI and ND Participants nformation available from organiser (AHVLA, UK) Information available from organiser (AHVLA, UK) 1 validation of a diagnostic protocol: specify the ; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? Title of the project or contract Scope Name(s) of relevant OIE Reference Laboratories EMIDA ERA-Net Epi-Seq epizootic diseases using next generation sequencing technology Friedrich-Loeffler-Institut (Germany) ANIHVA- NADIV Cooperation and coordination of National Research Programmes on Animal Health and Welfare of farm animals, including fish and bees Kimron Veterinary Institute (Israel);SVA-Statens Veterinamedicinska Anstalt (Sweden) EPIZONE Prevention and control of Animal Diseases Friedrich-Loeffler-Institut (Germany);Veterinamedicinska Anstalt (Sweden);Istituto Zooprofilattico Sperimentale della Lombardia e dell'emilia Romagna 8 OIE Reference Laboratory Reports Activities, 2013
9 ToR: To organise inter-laboratory proficiency ing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency s with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory comparisons in: Laboratory Proficiency Testing at: see point 1.3 Purpose for inter-laboratory comparisons 1. participating laboratories Region(s) of participating OIE Member Countries FAO Proficiency for AI and Newcastle disease (organiser) 8 National Proficiency Test for AI and Newcastle Disease(organiser) 15 ToR: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? 25. Additional comments regarding your report: Publishing: Capua, I., and Alexander, D. J. Influenza aviaire et maladie de Newcastle. Un manuel de terrain et de laboratoire. Springer. March OIE Reference Laboratory Reports Activities,
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