Molecular diagnosis of infectious bronchitis: recent developments. Richard Currie
|
|
- James Nash
- 6 years ago
- Views:
Transcription
1 Molecular diagnosis of infectious bronchitis: recent developments Richard Currie
2 The infectious bronchitis virus RNA (nucleic acid) on the inside the genetic finger print Proteins (S1 and S2 spike proteins) on the outside blood tests detect antibodies made against these
3 By looking at the S1 gene and protein, we can fingerprint the virus
4 4/91 Field virus
5 4/91 Field virus nucleic acid sequence XXXX SAMPLE SEQUENCE XXXX SAMPLE SEQUENCE
6 Key point Genetic fingerprints allow us to differentiate viruses from one another We can CLEARLY diagnose different 793B vaccine strains They are not the same They behave differently We can differentiate vaccines from field viruses
7 The use of amino acid sequence to predict epitopes in the hypervariable region of the S1 protein An epitope is the major antigenic determinant of the virus: it s what antibodies bind to
8 The infectious bronchitis virus By understanding the genetic finger print of the live IB vaccines we have, we can differentiate field viruses from vaccine viruses We can determine if the genetic finger print of the vaccine virus changes Is it stable? Is it persistent? Does it colonise the farm and displace field viruses? Which vaccine strain given becomes dominant on-farm? Diversity of genetic finger prints of field viruses can be used to justify the use of combined Ma5 plus 4/91 protectotype vaccination strategies to generate the broadest protection possible
9 The x-ovo diagnostic process Real time PCR Is IB present YES or NO How much is present? Sequencing PCR and amino acid alignments Differentiation of vaccines from field viruses Interpretation, reporting and recommendations
10 European overview
11 Origin of Samples EMEA Broiler Parent 12% Layer 34% Broiler 48% Other 6% No of Submissions 2315 Other Category Includes - Partridge,Layer Parent, Pheasant and Not Classified
12 Most field viruses are QX and 4/91 genotypes % Homology All Countries 01/01/11-21/10/ /91 Ark CR88 D274 H120 Italy-02 Ma5 Other QX Negative SU 100% Sequencing Homology <100% Sequencing Homology Epitope Mismatches <100% Sequencing Homology No Epitope Mismatches Negative Results % Strain Untypeable Results% No of submissions 2555 Mismatches are differences in epitope location and composition between vaccines and field viruses
13 Most field viruses are found in commercial layers - EMEA 4/91 field viruses Layer 59% Broiler Parent 20% Broiler 13% Other 8% No of Submissions 253 Other Category Includes - Layer Parent and Not Classified,
14
15 Successful vaccination populates the broiler farm with 4/91 vaccine virus Poland 2012 % Well applied vaccination of 4/91 together with Ma5 leads to the establishment of 4/91 as the dominant strain on-farm, with no field viruses found /91 D274 H120 QX Tabic IB Var V % <100%
16 Field data from France
17
18 France 01/11/ /10/2013 France 01/11/ /10/2012 Negative 40% Positive 60% Negative 53% Positive 47% No of Submissions 95 No of Submissions 62
19
20
21 Most field viruses are 4/91 genotypes - France
22 Most field viruses are QX and 4/91 genotypes - Belgium
23 Most field viruses are QX and 4/91 genotypes - Germany % Homology 01/01/ /10/ /91 CR88 D274 H120 M41 Ma5 QX Neg SU 100% <100% Homology Mismatches <100% Homology No Mismatches SU% Neg No of Submissions Mismatches are differences in epitope location and composition between vaccines and field viruses
24 Summary There is a major difference between QX field virus incidence between France and some other EU countries In common with other countries, 4/91 genotype field virus remains the major threat Note that these field viruses are more closely related to the 4/91 genotype than the CR88 or FR94 genotypes An explanation of the difference in QX diagnoses will be given in detail towards the end of the day s presentation
25 What happens when you vaccinate a flock with infectious bronchitis vaccines? It depends on the strains you use
26 H120 only vaccination Origin of Samples Age at Sampling Type of Birds Tracheal & Cloacal 34d Broiler Vaccination History Vaccine Age Dose Route of Application H120 1d 1 Coarse Spray Molecular Analysis Molecular analysis identified the following quantities of IB viral RNA : Tracheal swab: Ct = Cloacal swab Ct = Sequencing Analysis Sequencing analysis characterised this virus as an H120 type infectious bronchitis virus with 100% nucleic acid homology and 100% amino acid homology to the known H120 vaccine vial sequence In general mass type vaccines appear to be recovered in larger quantities from the trachea than cloaca
27 Ma5 plus 4/91 1a Origin of Samples Age at Sampling Type of Birds Tracheal Cloacal Other 6d broiler 10 Vaccination History Vaccine Age Dose Route of Application Nobilis IB 4-91 Hatchery Nobilis IB Ma5 Hatchery Molecular Analysis Molecular analysis identified a large quantity of IB viral RNA (Ct = 21.52). Sequencing Analysis Sequencing analysis characterised this virus as an Ma5 type infectious bronchitis virus with 100% nucleic acid sequence homology and 100% amino acid sequence homology to the known Ma5 vaccine vial sequence Early sampling after combined administration can find Ma5.
28 Ma5 plus 4/91 1b Origin of Samples Age at Sampling Type of Birds Tracheal Cloacal Other 7d broiler Vaccination History Vaccine Age Dose Route of Application Nobilis IB 4-91 Hatchery Nobilis IB Ma5 Hatchery Molecular Analysis Molecular analysis identified the following quantities of IB viral RNA Sample 1 Tracheal Swabs RNA (Ct = 20.06) 4/91 type infectious bronchitis Sample 2 Cloacal Swabs RNA (Ct= 19.49) 4/91 type infectious bronchitis Sequencing Analysis Sequencing analysis characterised this virus as a 4/91 variant infectious bronchitis virus with 100% nucleic acid sequence homology and 100% amino acid sequence homology to the known 4/91 vaccine vial sequence.. Or 4/91
29 Ma5 plus 4/91-2 Origin of Samples Age at Sampling Type of Birds Tracheal Cloacal Other 24d broiler Vaccination History Vaccine Age Dose Route of Application Nobilis IB d 1 ds Spray Nobilis IB Ma5 01d 1 ds Spray Molecular Analysis Molecular analysis identified the following quantities of IB viral RNA Sample 1 Tracheal Swabs RNA (Ct =33.46) - Ma5 type infectious bronchitis Sample 2 Cloacal Swabs RNA (Ct= 24.91) 4/91 type infectious bronchitis Sequencing Analysis Sequencing analysis characterised this virus as Ma5 & 4/91 type infectious bronchitis viruses with 100% nucleic acid sequence homology and 100% amino acid sequence homology to the respective vaccine vial sequences. At times you can find both strains..
30 Ma5 plus 4/91-3 Origin of Samples Age at Sampling Type of Birds Tracheal Cloacal Other 31d broiler 10 Vaccination History Vaccine Age Dose Route of Application Nobilis IB Ma5 Day 1 spray Nobilis IB 4-91 Day 1 spray Poulvac IB QX Day 14 spray Molecular Analysis Molecular analysis identified a large quantity of IB viral RNA (Ct = 22.91). Sequencing Analysis Sequencing analysis characterised this virus as a 4/91 variant infectious bronchitis virus with 100% nucleic acid sequence homology and 100% amino acid sequence homology to the known 4/91 vaccine vial sequence. before 4/91 eventually establishes dominance
31 Microarray results support the effective disappearance of Ma5 when 4/91 dominance is established 3000 Results 2500 PROB 793B ITA02 E 624/I ARK NEV 2000 (MFI) B IT02 624/I ARK BS2-like M41 D274 IS-1494/06- like QX Q1 Negative Control
32 All Countries Broiler (<84 days) Vaccinated with 4/91 and Ma5 at Day of Age 08/09/11-11/02/2014 % /91 Ark D274 H120 Ma5 QX Other SU Neg 100% <100% Homology Mismatches <100% Homology No Mismatches SU % Neg % No of Results - 45
33 Comparison of 4/91 and CR88 vaccine behaviour Differences in persistence
34 100% homologous 4/91 vaccine virus
35 100% homologous CR88 vaccine virus
36 Arkansas - 1 Origin of Samples Age at Sampling Type of Birds Tracheal Cloacal Other 23d Broiler 5 Vaccination History Vaccine Age Dose Route of Application IBmm+Ark Day 0 1 dose Spray IB Primer Day 10 1 dose Spray Molecular Analysis Molecular analysis identified a small quantity of IB viral RNA (Ct = 30.39). Sequencing Analysis Sequencing analysis characterised this virus as an Ark variant infectious bronchitis virus with 100% nucleic acid homology and 100% amino acid homology to the know Ark vaccine vials sequence
37 Arkansas - 2 Origin of Samples Age at Sampling Type of Birds Tracheal Cloacal Other 28d Broiler 10 Vaccination History Vaccine Age Dose Route of Application IBmm+Ark 1d 1ds Spray Molecular Analysis Molecular analysis identified a significant quantity of IB viral RNA (Ct = 27.78). Sequencing Analysis Sequencing analysis characterised this virus as an Ark variant infectious bronchitis virus with 100% nucleic acid sequence homology and 100% amino acid sequence homology to the 4 th most prevalent sub population of viruses from the Ark vaccine vial
38 Arkansas 3 Ark979 Origin of Samples Age at Sampling Type of Birds Tracheal Cloacal Other 27d Broiler 10 Vaccination History Vaccine Age Dose Route of Application IBmm+Ark 0 days 1 spray Molecular Analysis Molecular analysis identified a significant quantity of IB viral RNA (Ct = 25.38). Sequencing Analysis Sequencing analysis characterised this virus as an Ark variant infectious bronchitis virus with the following substitutions from the known Ark vaccine vial sequence. Nucleic Acid Sequence Position Vaccine Vial Sequence Sample Sequence Comments 979 Thymine Adenine Amino Acid Sequence Position Vaccine Vial Hydrophobicity Index Sample Hydrophobicity Index Hydrophobic/ Hydrophilic Switch Sequence Sequence 327 Tyrosine -1.3 Asparagine -3.5 NO
39 Arkansas - 4 Origin of Samples Age at Sampling Type of Birds Cloacal 28d Broiler Vaccination History Vaccine Age Dose Route of Application H120+D274 Hatchery 1ds Spray Molecular Analysis Molecular analysis identified a large quantity of IB viral RNA (Ct = 23.03). Sequencing Analysis Sequencing analysis characterised this virus as an Ark variant infectious bronchitis virus with the following substitutions from the known Ark vaccine vial sequence. Nucleic Acid Sequence Position Vaccine Vial Sequence Sample Sequence Comments 979 Thymine Adenine 1037 Adenine Guanine Amino Acid Sequence Position Vaccine Vial Hydrophobicity Index Sample Hydrophobicity Index Hydrophobic/ Hydrophilic Switch Sequence Sequence 327 Tyrosine -1.3 Asparagine -3.5 NO 346 Asparagine -3.5 Serine -0.8 NO
40 Arkansas 5a multiple viruses, same flock Origin of Samples Age at Sampling Type of Birds Tracheal Cloacal Other 33d Broiler Vaccination History Vaccine Age Dose Route of Application IB mm Ark Day of age Spray CR88 Day 14 Drinking water Molecular Analysis Molecular analysis identified the following quantities of IB viral RNA Sample 1 Tracheal Swabs RNA (Ct = 30.14).- Ark type infectious bronchitis Sample 2 Cloacal Swabs RNA (Ct= 25.60) Ark type infectious bronchitis Nucleic Acid Sequence TRACHEAL SWAB Position Vaccine Vial Sequence Sample Sequence Comments 903 Cytosine Thymine 979 Thymine Adenine 1036 Adenine Guanine 1037 Adenine Cytosine Amino Acid Sequence TRACHEAL SWAB Position Vaccine Vial Hydrophobicity Index Sample Hydrophobicity Index Hydrophobic/ Hydrophilic Switch Sequence Sequence 327 Tyrosine -1.3 Asparagine -3.5 NO 346 Asparagine -3.5 Alanine 1.8 YES
41 Arkansas 5b multiple viruses, same flock Origin of Samples Age at Sampling Type of Birds Tracheal Cloacal Other 33d Broiler Vaccination History Vaccine Age Dose Route of Application IB mm Ark Day of age Spray CR88 Day 14 Drinking water Molecular Analysis Molecular analysis identified the following quantities of IB viral RNA Sample 1 Tracheal Swabs RNA (Ct = 30.14).- Ark type infectious bronchitis Sample 2 Cloacal Swabs RNA (Ct= 25.60) Ark type infectious bronchitis Nucleic Acid Sequence CLOACAL SWAB Position Vaccine Vial Sequence Sample Sequence Comments 979 Thymine Adenine Amino Acid Sequence CLOACAL SWAB Position Vaccine Vial Hydrophobicity Index Sample Hydrophobicity Index Hydrophobic/ Hydrophilic Switch Sequence Sequence 327 Tyrosine -1.3 Asparagine -3.5 NO
42 European Society for Veterinary Virology 2012
43 What happens when an incomplete dose of vaccine received by the chicken? With 4/91 there is a measurable effect regarding tissue distribution
44 Kidney swabs: Fractional dose versus full dose 4/91: Germany and the UK Drinking water vaccination
45 Comments The slide shows results from 4/91 vaccinated flocks All flocks are fractional dose unless stated D7, D21 and D35 4/91 positive results are spray vaccinated flocks from the UK One full dose 4/91 vaccinated flock in Germany (red circle) tested negative this was drinking water vaccinated and hence this route of administration, and hence administration failure may explain the negative finding In general, fraction dose vaccination does not lead to colonisation of the kidney following vaccination, a finding that would be expected with full dose spray vaccination Hence when clinical problems are found, careful auditing of vaccination to ensure the flock is exposed to a full dose of vaccine, is advised
46 Summary The multiplication of genetic take populations of vaccine viruses can be measured Dominance of the 4/91 strain is expected in the majority of instances Failure to detect the 4/91 strain is a trigger for vaccination application audits and specific sampling of the kidney 7-10 post vaccination to check for 4/91 vaccine replication Genetic and amino acid variation introduction to flocks vaccinated with Arkansas strains has been demonstrated in the UK similar to that seen in the USA Recombinant Ark 491 field viruses have been identified in flocks with clinical egg drop in the UK Correct identification of vaccine and vaccine origin strains is critical to determine correct vaccination strategies
47 Use of amino acid profiles to investigate epidemiology of IB variants that are not homologous with vaccine virus take populations
48 Findings The following graphs show the location and number of amino acid changes that occur on the S1 protein for 4/91, Mass and D274 genotypes Sample sets are subdivided into those obtained from young birds (<18 weeks of age) and laying birds (>18 weeks of age) In most amino acid positions, amino acid changes are very variable. Occasionally they are not.
49 4/91 Non homologous viruses Position of Amino Acid Changes in 4/91 Field Virus <18w of Age Position of Amino Acid Changes in 4/91 Field Virus >18w of Age 25 No of changes 64 No of Submissions No of changes 526 No of Submissions
50 Mass Non homologous viruses Position of Amino Acid Changes in Mass Field Virus <18w of Age Position of Amino Acid Changes in Mass Field Virus >18w of Age
51 D274 Non homologous viruses Position of Amino Acid Changes in D274 Field Virus <18w of Age Position of Amino Acid Changes in D274 Field Virus >18w of Age Position 304 Histidine - Tyrosine Position 304 Histidine - Tyrosine No of Changes No of Changes <18wks No of changes - 53 No of Submissions - 32 >18wks No of changes 53 No of submissions 47
52 Summary 4/91 and Mass field viruses observed in EMEA from show extensive changes relative to the take population of the respective vaccines The major incidence is seen in flocks over 18 weeks of age Introductions in broiler flocks show viruses with much fewer amino acid substitutions This observation is consistent with the introduction of amino acid variation during the course of field infections. The longer the field virus is present in a flock, the greater the amino acid variation that is seen These genotypes act as true field viruses
53 Summary D274 positives are present in relatively minor quantities The amino acid variation in D274 positives is very small 50% of all amino acid substitutions featured a single Histidine Tyrosine mutation at position 304. The mutation is not part of any defined epitope hence of minor immunological relevance Of this specific mutation, 70% of observations occurred in flocks known to be vaccinated with D274 plus H120 live vaccine
54 Summary The most probable diagnosis of the Histidine Tyrosine virus is therefore D274 vaccine origin The finding does not suggest in any way that the D274 strain is reverting to virulence This finding gives further support for the lack of significant D274 field challenge in the EMEA The importance of this finding relates to the prevention of the vaccinating against vaccine strategy
55 Vaccinate against true field viruses Advanced next generation sequencing techniques (NGS) Most field viruses are QX and 4/91 genotypes - Germany Allow clear differentiation of vaccines from field viruses When applied to field outbreaks, can clarify the apparent large number of field viruses found and allow their re-classification In the case of QX, demonstrates the over diagnosis of QX field viruses by around 50% - GERMAN EXAMPLE OPPOSITE % Homology 01/01/ /10/2013 4/91 CR88 D274 H120 M41 Ma5 QX Neg SU 100% <100% Homology Mismatches <100% Homology No Mismatches SU% <100% Homology Vaccine Sub Population No of Submissions - 211
56 QX vaccine in its minor forms is less prevalent in flocks where 4/91 is also given Vaccine origin
57 QX vaccine in its minor forms is more prevalent in flocks where 4/91 is not given Vaccine origin
58 It is normal for live IB vaccines to spread to flocks not vaccinated directly QX vaccine origin positives occur in nonvaccinated flocks as well as QX vaccinated flocks
59 Prevalence of IB vaccines in non vaccinated flocks
60 Summary The presence in the field of minority point mutations present in live QX vaccine vials has been demonstrated by NGS sequencing The mutations are present in QX vaccinated flocks at times where a registered duration of immunity is present. If the viruses were field viruses, the vaccine would be predicted to be protective The mutations are present in the field in countries that use the largest quantities of QX vaccine and absent from countries where usage is minor. The viruses are found in the presence and the absence of live QX vaccine.
61 Summary Statistical analysis of the sequences obtained indicates the present of section pressure (consistent with the viruses being of vaccine origin) for positions 429 and 485 (partial sequence numbering)/ codons 117 and 131 (full sequence numbering) The consequences of this finding are the over diagnosis of QX field viruses that should be reclassified as vaccine origin viruses. In Belgium and Germany this is occurring in around 50% of cases of a QX diagnosis The findings do not relate in any way to the presence or absence of a reversion to virulence
62 Conclusion Molecular analysis of clinical samples for the presence of IB can Diagnose the presence of field strains relative to live vaccine strains. Demonstrate differences in behaviour between the major viral genotypes. Identify amino acid changes that are associated with clinical disease. Suggest the application of Protectotype vaccination programmes when field viruses with amino acid characteristics the diverge greatly from vaccine strains are present, thus leading to epitope changes relative to vaccine strains.
63 Conclusion The use of 4/91 based vaccination programmes results in the population of the flock with 4/91 vaccine strain as the dominant strain on farm that is persistent and consistent from a genetic perspective The use of repeat spray in lay vaccination programmes will ensure the maintenance of significant quantities of vaccine virus RNA throughout the laying period and thus provide significant antigen quantities against which a protective immune response will be generated Flocks in lay are confirmed as the major source of infectious bronchitis field viruses. Thus layer flocks in the vicinity of broiler flocks remain a major risk for the introduction of field virus infections
64 Evaluation of the field efficacy of vaccination strategies 1. Broilers
65 What vaccination programmes are associated with field viruses in broilers (>42 days)? EU countries only Negative results in young birds with any correctly administered bronchitis vaccination programme and proper sampling technique should not occur key investigation point SU 8% Neg 18% Short lived broilers 01/02/ /02/2013 Field Virus 76 25% Vaccine Virus %
66 What vaccination programmes are associated with field viruses in broilers (>42 days)?- EU countries only Short lived broilers - all results 01/02/ /02/2013 Other Vaccines 48% 4/91 + anything else 29% 4/91 & Ma5 15% Ma5 +anything else 8%
67 What vaccination programmes are associated with field viruses in broilers (>42 days)? EU countries only Broilers - Field Virus 01/02/ /02/2013 Field Virus with 4/91 + anything else 23% Field Virus with 4/91 & Ma5 4% Other Vaccines 73%
68 Evaluation of the field efficacy of vaccination strategies 2. Birds in lay
69 EMEA field results flocks over 18 weeks of age Results for 18w & Over Submissions 27/04/ /04/2013 Key conclusion: The most likely field virus finding in lay is 4/91 followed by QX
70 Incidence of field viruses Total number of inlay submissions (>18 weeks of age) where 4/91 field virus was confirmed 107 Number of inlay submissions (>18 weeks of age) where 4/91 field virus was confirmed no live IB vaccination in lay 63 Number of inlay submissions (>18 weeks of age) where 4/91 field virus was confirmed and live IB vaccination was used in lay 44 In lay vaccination is associated with the presence of fewer field viruses
71 Administration pattern of Ma5 and 4/91 in lay Distance between 4/91 and Ma5 13/06/ /04/ days; 18; 30% >14 days; 42; 70% Tendency to give Ma5 and 4/91 further apart than optimum for cross protection response
72 Field virus examples 2013
73 Key question How do we know a field virus is causing disease? This is determined by the appearance of clinical signs. The results give supporting indications of the potential of the virus to be a disease causing agent
74 Use of phylogenetic tree to establish the presence of a virus that differs significantly
75 4/91 Field virus broilers epitope mismatch with vaccine Individual tracheal (Ct 27.91) and cloacal (Ct 21.62) swab analysis. H120 at day 7, 4/91 at day 20 Vaccination History Vaccine Age Dose Route of Application HB1+IB 7 days 1ds DW Bursine plus 12 days 1ds DW ND Inac. 18 days 1ds Inj. 4/91 20 days 1ds Eye drop Respiratory clinical signs, mortality, enteritis. Large quantity of 4/91 field virus (9 amino acid substitutions different from the vaccine) shedding prior to onset of immunity of 4/91 vaccine. Recommendation to move 4/91 plus Ma5 application to day of age
76 QX field virus EU cloacal swabs - epitope mismatch with vaccine 67 week old layers vaccinated with 4/91 at 14d & 10w, Ma5 at 1d, 7w & 14w Ct Value Vaccination History Vaccine Age Dose Route of Application Nobilis IB d; 10 w 1 ds aerosol Nobilis IB H120 Nobilis IB Ma5 1d; 7w; 14w 1 ds aerosol Nobilis IB inac (M41) 16w 1 ds inj Ct value not consistent with aggressive infection
77 QX field virus EU cloacal swabs - epitope mismatch with vaccine 53 week old layers vaccinated with 4/91 at 14d & 8w, Ma5 at 35d and Primer at 1d & 10w Ct Value Vaccination History Vaccine Age Dose Route of Application Nobilis IB d,8 wks 1ds Spray, drinking water Nobilis IB H120 Nobilis IB Ma5 35d 1ds Drinking water H120+D274 (IB Primer) 1 d, 10 wks 1ds Spray, drinking water No clear IB related clinical signs sampling from another house on the same farm showed small quantities of untypable virus. Protectotype vaccination programme is not optimised for QX protection
78 4/91 field virus EU cloacal swabs - epitope mismatch with vaccine 74 week old broiler parents vaccinated with 4/91 at 14d & 10w, H120 at 14w & 56w, Ma5 at 35d and IB Primer at 1d and 19w: Ct Value Vaccination History Vaccine Age Dose Route of Application Nobilis IB d;10w 1 ds spray H120 14w;56w 1ds spray Nobilis IB Ma5 35d 1 ds spray H120+D274 (IB Primer) 1d; 19w 1 ds spray IB88 IBmm+Ark Nobilis IB inac (M41) Olvac A+B (Nev14+Nev24) inac 69w 1ds inj Nobilis IB multi inac (M41+D274) 16w 1ds inj No 4/91 vaccine in close proximity to field challenge
79 4/91 field virus EU cloacal swabs - epitope mismatch with vaccine 53 week old broiler parents vaccinated with 4/91 at 14d & 10w, Ma5 at 8w and IB Primer at 1d: Ct Value Vaccination History Vaccine Age Dose Route of Application Nobilis IB days, Coarse spray 1 ds 10 weeks fine spray Nobilis IB H120 Nobilis IB Ma5 8 weeks 1 ds Fine spray H120+D274 (IB Primer) 1 day 1 ds Coarse spray IB88 IBmm+Ark Nobilis Rhino CV 6 weeks 1 ds Coarse spray NobilisTRT Live Other (please specify) Nobilis RT+IB multi+g_nd 18 weeks 1d Inj.i.m. No 4/91 vaccine in close proximity to field challenge
80 MASS Type field virus EU cloacal swabs - epitope mismatch with vaccine 64 week old layers vaccinated with 4/91 at 2w & 9w, Ma5 at 5w & 11w: Ct Value Vaccination History Vaccine Age Dose Route of Application Nobilis IB ,9 wks 1 ds spray Nobilis IB H120 Nobilis IB Ma5 5, 11 wks 1 ds Drinking water H120+D274 (IB Primer) IB88 IBmm+Ark Nobilis Rhino CV NobilisTRT Live ( Nemovac) 14 wks 1 ds spray Other (please specify) Ceva Bron 1 d 1 ds spray
81 QX field virus EU cloacal swabs - epitope mismatch with vaccine 64 week old layers vaccinated with 4/91 at 2w & 9w, Ma5 at 5w & 11w Ct Value Vaccination History Vaccine Age Dose Route of Application Nobilis IB ,9 wks 1 ds spray Nobilis IB H120 Nobilis IB Ma5 5, 11 wks 1 ds Drinking water H120+D274 (IB Primer) IB88 IBmm+Ark Nobilis Rhino CV NobilisTRT Live ( Nemovac) 14 wks 1 ds spray Other (please specify) Ceva Bron 1 d 1 ds spray
82
Worldwide perspective on Infectious Bronchitis. Ruth Bouwstra, DVM, PhD Turkey February 2017
Worldwide perspective on Infectious Bronchitis Ruth Bouwstra, DVM, PhD Turkey February 2017 Infectious bronchitis virus Corona Virus, a ssrna virus - Relatively high rate of mutations (0,0012 subst per
More informationInfectious Bronchitis: how to maximize cross-protection. GD Animal Health - The Netherlands
Infectious Bronchitis: how to maximize cross-protection GD Animal Health - The Netherlands Infectious Bronchitis: how to maximize cross-protection J.J. (Sjaak) de Wit, DVM, PhD, dipl ECPVS GD Animal Health
More informationIBV HOW TO DEAL WITH TODAY S MOST RELEVANT FIELD STRAINS WITHIN THE EUAFME REGION?
IBV HOW TO DEAL WITH TODAY S MOST RELEVANT FIELD STRAINS WITHIN THE EUAFME REGION? IB VARIANTS HISTORY EUAFME PERSPECTIVE 1930 s: Massachussetts (mortality 40-90%) 2 1951: first variant in US: Connecticut
More informationDepartment of Veterinary Pathology, University of Liverpool, Leahurst Campus, Neston, South Wirral, CH64 7TE, UNITED KINGDOM
IMMUNE RESPONSES IN CHICKS AFTER SINGLE OR DUAL VACCINATION WITH LIVE INFECTIOUS BRONCHITIS MASSACHUSETTS AND VARIANT VACCINES: SOME PRELIMINARY FINDINGS GANAPATHY 1 K, ROTHWELL 2 L, LEMIERE 3 S, KAISER
More informationSuggestions to prevent / control Respiratory Disease Complex in poultry
Suggestions to prevent / control Respiratory Disease Complex in poultry Dr. J. L. Vegad Adviser Phoenix Group 201/15, Gorakhpur, Jabalpur - 482001 Introduction Today, respiratory disease complex has emerged
More informationSCIENTIFIC DISCUSSION EMEA/V/C/036. Intervet International B.V., Wim de Körverstraat 35, 5831 AN Boxmeer, The Netherlands. N/a
SCIENTIFIC DISCUSSION Product name: Nobilis IB 4-91 Procedure No.: EMEA/V/C/036 Applicant company : Active substances and strengths: (ATCvet code) Proposed International Non-proprietary Name: Intervet
More informationFALSE LAYERS UPDATE EGG & PULLET FARMERS WORKSHOP 2017 DAN VELDMAN
FALSE LAYERS UPDATE EGG & PULLET FARMERS WORKSHOP 2017 DAN VELDMAN One of these hens is a false layer which one? False Layer Layer DEFINITION False Layer (Poultry) : a hen with the appearance and behavior
More informationCevaC Mass L THe Ceva Mass solution
CevaC Mass L THe Ceva Mass solution Ceva Santé Animale S.A. - 10, av. de la Ballastière - BP 126-33500 Libourne Cedex - France Tel : + 33 5 57 55 40 40 - Fax : + 33 5 57 55 41 92 www.ceva.com CEVAC MASS
More informationRECOMBINANT VACCINES Live or Killed
Antigens and Vaccines Molecular biology drives Innovation Antigen Gene of Interest Recombination RECOMBINANT VACCINES Live or Killed Antigens and Vaccines Molecular biology drives Innovation Antigen Gene
More informationSCIENTIFIC DISCUSSION
SCIENTIFIC DISCUSSION 1. SUMMARY OF THE DOSSIER Nobilis Influenza H5N2 emulsion for injection, is an adjuvanted, inactivated vaccine against avian influenza type A, subtype H5 in chickens. Avian influenza
More informationCEVAC CEVAC BROILER ND K / 5000 doses
CEVAC BROILER ND K / 5 doses CEVAC BROILER ND K Newcastle disease a permanent threat Newcastle Disease is highly contagious and exists in a wide range of forms. It is one of the four major poultry diseases
More informationGumboro Disease: where are we with IBDV epidemiology. J.J. (Sjaak) de Wit, DVM, PhD, dipl ECPVS GD Deventer, The Netherlands
Gumboro Disease: where are we with IBDV epidemiology J.J. (Sjaak) de Wit, DVM, PhD, dipl ECPVS GD Deventer, The Netherlands Gumboro-virus (IBDV) Avibirna-virus: 2 segments of dsrna Non enveloped virus
More informationANNEX I SUMMARY OF PRODUCT CHARACTERISTICS
ANNEX I SUMMARY OF PRODUCT CHARACTERISTICS 1 1. NAME OF THE VETERINARY MEDICINAL PRODUCT Nobilis IB Primo QX lyophilisate and solvent for suspension for chickens Nobilis IB Primo QX lyophilisate for suspension
More informationLaboratory tools for monitoring and understanding IBDV infection and vaccination
Laboratory tools for monitoring and understanding IBDV infection and vaccination J.J. (Sjaak) de Wit, DVM, PhD, dipl ECPVS GD Deventer, The Netherlands Gumboro-virus (IBDV) Avibirna-virus: segments of
More informationLimitation of the spread and impact of infectious coryza through the use of a continuous disinfection programme
Onderstepoort Journal of Veterinary Research, 7: 8 () Limitation of the spread and impact of infectious coryza through the use of a continuous disinfection programme R.R. RGG Department of Microbial, iochemical
More informationANNEX I SUMMARY OF PRODUCT CHARACTERISTICS
ANNEX I SUMMARY OF PRODUCT CHARACTERISTICS 1 1. NAME OF THE VETERINARY MEDICINAL PRODUCT Nobilis IB 4-91 lyophilisate for suspension for chickens 2. QUALITATIVE AND QUANTITATIVE COMPOSITION Each dose of
More informationEvolution of influenza
Evolution of influenza Today: 1. Global health impact of flu - why should we care? 2. - what are the components of the virus and how do they change? 3. Where does influenza come from? - are there animal
More informationVector Newcastle vaccine usage in Latin America. Luiz Sesti, Technical Services Latin America Ceva, Brazil
Vector Newcastle vaccine usage in Latin America. Luiz Sesti, Technical Services Latin America Ceva, Brazil Countries with endemic velogenic Newcastle Disease (ND) in Latin America Mexico Guatemala Belize
More informationField experience with vaccination in turkeys in Morocco. Taoufik RAWI M.C.I Santé Animale (Morocco)
Field experience with vaccination in turkeys in Morocco Taoufik RAWI M.C.I Santé Animale (Morocco) Field experience with vaccination in turkeys in Morocco Taoufik Rawi, DVM, MBA Head Of Technical Support
More informationVaccination to stop transmission
Vaccination to stop transmission Arjan Stegeman j.a.stegeman@uu.nl Faculty of Veterinary Medicine Goals of vaccination To prevent clinical disease (production, animal welfare, treatment costs, antimicrobial
More informationCONVENIENCE PROGRAM. An early start in respiratory health for a more profitable life.
CONVENIENCE PROGRAM An early start in respiratory health for a more profitable life. The Convenience Program puts our science and people to work for you Respiratory disease is a global threat to the health
More informationDr.Christophe Cazaban
Dr.Christophe Cazaban Technical Manager Biology Innovation Strategy Department. Ceva Sante Animale, France Graduated from the French Veterinary University of Lyon in 1993 He completed his education with
More informationCeva s offer to optimize performance Simpler vaccination and better safety
Ceva s offer to optimize performance Simpler vaccination and better safety Rick van Oort Poultry Corporate Range Manager Layer range CEVA Sante Animale Bangkok-Thailand March 12, 2013 Bodyweight (g) 1600
More informationThe Review of Newcastle Disease Live Vaccines Application. By: M. Abdoshah
The Review of Newcastle Disease Live Vaccines Application By: M. Abdoshah Introduction Only one serotype (PMV1) Maternally derived antibodies are protective All vaccines are efficacious Is vaccine protection
More informationMG and MS Control in Layers
MG and MS Control in Layers Bernie Beckman, DVM Hy-Line International Hy-Line International Genetic Excellence Respiratory Diseases of Poultry Bacterial Diseases M. gallisepticum M. synoviae Coryza - Avibacterium
More informationImmunity and Poultry Health (3)
Understanding vaccines and vaccination programmes Protecting the health of poultry through vaccination has been an essential part of poultry production for more than 50 years. Vaccination is the final
More informationMYCOPLASMOSIS - A SERIOUS PROBLEM OF POULTRY INDUSTRY
By, Dr. J. L. Vegad Advisor Phoenix Group MYCOPLASMOSIS - A SERIOUS PROBLEM OF POULTRY INDUSTRY Mycoplasmosis, commonly known as chronic respiratory disease of chickens, has existed in our country since
More informationEtiology. Paramyxovirus type 1 = Newcastle disease.
Newcastle Disease Many strains of similar virus cause signs ranging from mild respiratory signs (pneumotropic) with low mortality to severe neurological (neurotropic) and/or visceral lesions (viscerotropic)
More informationHome News Chickens Turkeys Processing Other Poultry Regions Even
Page 1 of 5 Home News Chickens Turkeys Processing Other Poultry Regions Even Search Newsletter You are here: Home» Early detection for effective broody control» Early detection for effective broody control»
More informationAviagenBrief. Marek s Disease Control in Broiler Breeders
AviagenBrief January 2018 Marek s Disease Control in Broiler Breeders Author: A. Gregorio Rosales DVM, MS, PhD, DACPV - Poultry Health Consultant Introduction Marek s Disease Virus (MDV), a highly infectious
More informationmaking LT protection safer and easier
making LT protection safer and easier 1 vaccine up to 3 immunities 4 Vectormune FP-LT and. Vectormune FP-LT is a genetically engineered live fowl pox virus vaccine carrying 2 immunorelevant genes from
More informationWeekly Influenza Surveillance Report. Week 11
Weekly Influenza Surveillance Report Week 11 Report produced: 22/03/2001 Influenza activity in Ireland For the week ending the 18/03/01, week 11, influenza activity has increased. Sentinel general practices
More informationINFLUENZA-2 Avian Influenza
INFLUENZA-2 Avian Influenza VL 7 Dec. 9 th 2013 Mohammed El-Khateeb Overview 1. Background Information 2. Origin/History 3. Brief overview of genome structure 4. Geographical Distribution 5. Pandemic Nature
More informationCOMMITTEE FOR MEDICINAL PRODUCTS FOR VETERINARY USE (CVMP)
European Medicines Agency Veterinary Medicines and Inspections London, 16 February 2006 Doc. Ref.EMEA/CVMP/IWP/46853/2006 COMMITTEE FOR MEDICINAL PRODUCTS FOR VETERINARY USE (CVMP) REFLECTION PAPER: MINIMUM
More informationAvian influenza Avian influenza ("bird flu") and the significance of its transmission to humans
15 January 2004 Avian influenza Avian influenza ("bird flu") and the significance of its transmission to humans The disease in birds: impact and control measures Avian influenza is an infectious disease
More informationInterpretation and Application of Results Manual
Interpretation and Application of Results Manual Contents Applications of Results Obtained from BioChek ELISA kits page 3 Good sampling and Sample Size page 4-8 Interpretation of BioChek Results page 9
More informationVectormune ND a step towards control of Newcastle Disease
Poultry Vaccinology Summit April 28-30, Budapest, Hungary Vectormune ND a step towards control of Newcastle Disease Yannick Gardin 1, Vilmos Palya 1, John Elattrache 1, Marcelo Paniago 2, Christophe Cazaban
More informationCorrelation of humoral and cellular immune responses induced by different IBV vaccination regime with the protection afforded against IBV Q1
Correlation of humoral and cellular immune responses induced by different IBV vaccination regime with the protection afforded against IBV Q1 Rajesh Chhabra 1,2, Anne Forrester 1,Stephane Lemiere 3, Julian
More informationSalt Intoxication in Commercial Broilers and Breeders a Clinical and Pathological Description
Salt Intoxication in Commercial Broilers and Breeders a Clinical and Pathological Description Perelman, B., 1 * Farnoushi, Y., 2 Krispin, H. 3 and Rish, D. 4 1 Poultry Veterinarian-Clinical Consultant,
More informationThis paper is in two Sections (A and B) and instructions relating to the number of questions to be answered are given at the head of each Section.
TUESDAY 28 MARCH 2000 PAPER I (3 hours) This paper is in two Sections (A and B) and instructions relating to the number of questions to be answered are given at the head of each Section. SECTION A Two
More informationEUROPEAN COMMISSION HEALTH & CONSUMERS DIRECTORATE-GENERAL. Unit G5 - Veterinary Programmes
EUROPEAN COMMISSION HEALTH & CONSUMERS DIRECTORATE-GENERAL Unit G5 - Veterinary Programmes SANCO/10778/2012 Programmes for the eradication, control and monitoring of certain animal diseases and zoonoses
More informationIs Newcastle disease still a threat for the poultry industry?
Is Newcastle disease still a threat for the poultry industry? Marcelo PANIAGO Director Global Veterinary Services - Poultry Ceva Santé Animale Libourne - France Newcastle Disease in the world Newcastle
More informationAvian Influenza Virus H7N9. Dr. Di Liu Network Information Center Institute of Microbiology Chinese Academy of Sciences
Avian Influenza Virus H7N9 Dr. Di Liu Network Information Center Institute of Microbiology Chinese Academy of Sciences Avian Influenza Virus RNA virus, Orthomyxoviruses Influenza A virus Eight Gene segments
More information16 th JPC REM ESA M ohammedia 18-19th April Surveillance of low pathogenic Avian Influenza virus
16 th JPC REM ESA M ohammedia 18-19th April 2018 Surveillance of low pathogenic Avian Influenza virus DR.SSA BENEDETTA CAPPELLETTI, DVM VETERINARY OFFICER FOR EXPORT AND INTERNATIONAL AFFAIRS DIRECTORATE
More informationPoultry Disease Manual Characteristics And
Poultry Disease Manual Characteristics And Control Of Infections Written by: Dr. Jacquie Jacob, University of Kentucky Pullorum disease, also called Infection by Salmonella pullorum has also been reported
More informationCeva HAND book of poultry diseases
IVAN DINEV & CEVA Volume 1 Ceva HAND book of poultry diseases GUMBORO CHICKEN ANaEMIA REOVIRUS MAREK RUNTING-STUNTING SYNDROME NEWCASTLe DISEASE AVIAN INFLUENZA INFECTIOUS BRONCHITIS LARyNGOTRACHEITIS
More informationINCLUSION BODY HEPATITIS UPDATE: SEROTYPES, CONTROL AND PREVENTION
INCLUSION BODY HEPATITIS UPDATE: SEROTYPES, CONTROL AND PREVENTION Davor Ojkic, DVM, MSc, PhD Animal Health Laboratory University of Guelph Ontario, Canada Animal Health Laboratory Bacteriology Pathology
More informationBeurteilungsbericht zur Veröffentlichung. (gemäß 31 Abs. 2 Tierimpfstoff-Verordnung) Poulvac IB Primer
Beurteilungsbericht zur Veröffentlichung (gemäß 31 Abs. 2 Tierimpfstoff-Verordnung) Zulassungsdatum: 06.03.2000 Zulassungsnummer: 167a/88 Datum der Erstellung des öffentlichen Beurteilungsberichts: 15.05.2013
More informationPrinciples for event based and active avian influenza surveillance. Les Sims Asia Pacific Veterinary Information Services
Principles for event based and active avian influenza surveillance Les Sims Asia Pacific Veterinary Information Services apvis@bigpond.net.au Introduction No one size fits all surveillance system for avian
More informationOriginal Article Identification of Different Serotypes of Infectious Bronchitis Viruses in Allantoic Fluid Samples with Single and Multiplex RT- PCR
Iranian Journal of Virology 2009;3(2): 24-29 2009, Iranian Society for Virology Original Article Identification of Different Serotypes of Infectious Bronchitis Viruses in Allantoic Fluid Samples with Single
More informationMonitoring for Mycoplasma
Monitoring for Mycoplasma in vaccinated and non-vaccinated Poultry flocks Dr. Bart van Leerdam, PhD Prevention and Control Tactics of Mycoplasma spp. in Poultry 1. Maintaining flocks free of Mycoplasma
More informationManaging Salmonella Risk. Carl Heeder, DVM
Managing Salmonella Risk Carl Heeder, DVM 1 Overview Levels of Disease Risk Management Disease Risk Equation Flock Immunity Exposure Risk Salmonella Challenge Data Salmonella enteriditis Salmonella heidelberg
More informationModeling and Quantitative Risk Analyses to Support Business Continuity
UMN Secure Food System Team Food system solutions through risked based science Modeling and Quantitative Risk Analyses to Support Business Continuity Sasidhar Malladi 1, Peter Bonney 1, J. Todd Weaver
More informationINFLUENZA VIRUS. INFLUENZA VIRUS CDC WEBSITE
INFLUENZA VIRUS INFLUENZA VIRUS CDC WEBSITE http://www.cdc.gov/ncidod/diseases/flu/fluinfo.htm 1 THE IMPACT OF INFLUENZA Deaths: PANDEMICS 1918-19 S p a n is h flu 5 0 0,0 0 0 U S 2 0,0 0 0,0 0 0 w o rld
More information636.09: : :614.91:636.5
636.09:616.233-002:578.834:614.91:636.5.,..,. : ( ), ( ),,,.,.,,,, [1].,,.. ,,,.,,, [4].,,. :,.. ( ),,,., 7 45. 30-60-., 105 [2,3].,, . ( ) 7. 1930, A. Schalk i M.Hawn 1931., 1933. L. Buchelli i C. Brandly.
More informationMS-H Vaccine Eyedrop Suspension
MS-H Vaccine Eyedrop Suspension Vaccination against Mycoplasma synoviae using MS-H strain, live temperature sensitive vaccine Dr Peter Cargill BVetMed Cert PMP MRCVS Introduction Review of Mycoplasma synoviae
More informationANSES. Agence Nationale du Médicament Vétérinaire (National Agency for Veterinary Drugs) (Reference Member State) BP FOUGERES CEDEX FRANCE
ANSES Agence Nationale du Médicament Vétérinaire (National Agency for Veterinary Drugs) (Reference Member State) BP 90203 35302 FOUGERES CEDEX FRANCE DECENTRALISED PROCEDURE PUBLICLY AVAILABLE ASSESSMENT
More informationOIE Situation Report for Highly Pathogenic Avian Influenza
OIE Situation Report for Highly Pathogenic Avian Influenza Latest update: 30/06/2018 The epidemiology of avian influenza (AI) is complex. The AI virus constantly evolves by mutation and re-assortment with
More informationInfectious Bursal Disease, Immunosuppression and the role of VAXXITEK HVT+ IBD
Research note Infectious Bursal Disease, Immunosuppression and the role of VAXXITEK HVT+ IBD Grogan K. 1 1 Poultry Chicken Scratch, LLC, 30019 Dacula GA United States of America [from Hoerr F.J., 2010,
More informationIntroduction to Avian Influenza
Introduction to Avian Influenza David L. Suarez D.V.M., Ph.D. Research Leader Exotic and Emerging Avian Viral Disease Research Unit Agricultural Research Service United States Department of Agriculture
More informationMMCS Turkey Flu Pandemic Project
MMCS Turkey Flu Pandemic Project This is a group project with 2 people per group. You can chose your own partner subject to the constraint that you must not work with the same person as in the banking
More informationKeeping first class eggs flying high.
Keeping first class eggs flying high. www.msd-animal-health.com Mycoplasma synoviae (MS) in chickens MS IS A GLOBAL CHALLENGE AND OUTBREAKS HAVE BEEN SEEN AROUND THE WORLD SINCE THE 196 S MS starts as
More informationOIE Situation Report for Highly Pathogenic Avian Influenza
OIE Situation Report for Highly Pathogenic Avian Influenza Latest update: 31/05/2018 The epidemiology of avian influenza (AI) is complex. The AI virus constantly evolves by mutation and re-assortment with
More informationhttp://www.ibs.upm.edu.my Challenges in controlling viral diseases of poultry Abdul Rahman Omar Institute of Bioscience Faculty of Veterinary Medicine Universiti Putra Malaysia aro@upm.edu.my Outline of
More informationModule 1 : Influenza - what is it and how do you get it?
Module 1 : Influenza - what is it and how do you get it? Responsible/facilitators General Objective Specific Objectives Methodology Agency medical coordinator Understand the mechanism how influenza is
More informationDiagnostic Considerations for Marek s Disease. Frederic J. Hoerr, DVM, PhD AMEVEA - Argentina Colon, Entre Rios May 15, 2013
Diagnostic Considerations for Marek s Disease Frederic J. Hoerr, DVM, PhD AMEVEA - Argentina Colon, Entre Rios May 15, 2013 Control of Marek s Disease Accurate diagnosis Apply appropriate procedures to
More informationWPSA & WVPA Scientific Conference Roberto Soares, DVM, MAM, ACPV Regional Technical Manager - Poultry Ceva Animal Health APAC Malaysia
WPSA & WVPA Scientific Conference 2013 Roberto Soares, DVM, MAM, ACPV Regional Technical Manager - Poultry Ceva Animal Health APAC Malaysia Outlook the presentation Major changes in the Asia Poultry Industry
More informationINCLUSION BODY HEPATITIS AND HYDROPERICARDIUM SYNDROME (ADENOVIRUS INFECTIONS)
INCLUSION BODY HEPATITIS AND HYDROPERICARDIUM SYNDROME (ADENOVIRUS INFECTIONS) AVIAN ADENOVIRUSES (CHICKEN ADENOVIRUSES, FADV) Adenoviruses are common in poultry. Many replicate in healthy birds without
More informationAvian Influenza. Regional Workshops: Veterinary Discussion. Will Garton
Avian Influenza Regional Workshops: Veterinary Discussion Will Garton What is Avian Influenza? Influenza virus types A B C BIRDS, MAMMALS (including humans, pigs, horses, mink, sea mammals etc) HUMANS
More informationEU REFERENCE LABORATORIES FOR AVIAN INFLUENZA AND NEWCASTLE DISEASE
EUROPEAN COMMISSION HEALTH & CONSUMERS DIRECTORATE-GENERAL G2- Animal Health 04 Veterinary Control programmes SANCO/7048/204 Draft Working document EU REFERENCE LABORATORIES FOR AVIAN INFLUENZA AND NEWCASTLE
More informationDepartment of Animal and Poultry Sciences October 16, Avian Leukosis Virus Subgroup J. Héctor L. Santiago ABSTRACT
Department of Animal and Poultry Sciences October 16, 2000 Avian Leukosis Virus Subgroup J Héctor L. Santiago ABSTRACT The avian leukosis viruses (ALV) are a class of retroviruses belonging to the avian
More informationHealth Products Regulatory Authority
1 NAME OF THE VETERINARY MEDICINAL PRODUCT Nobilis ND Clone 30 live 2 QUALITATIVE AND QUANTITATIVE COMPOSITION Active substance: per dose * Live Newcastle disease virus, strain Clone 30 ³ 6.0 log 10 ELD
More informationOsHV-1 μvar. Part II. Annual Meeting NRLs for mollusc diseases La Rochelle, March 2011 Sigrid Cabot, DG SANCO
OsHV-1 μvar Part II Annual Meeting NRLs for mollusc diseases La Rochelle, March 2011 Sigrid Cabot, DG SANCO sigrid.cabot@ec.europa.eu Background Overview of the presentation Measures adopted 2010 EFSA
More informationGeneral context and objectives of the project. Public private partnership (PPP) in Veterinary Public Health. EVADOC project Bangladesh Jan-June 2015
Modeling the effectiveness of avian influenza vaccination strategies in Bangladesh: evaluation of the added value of day old chick vaccination in hatcheries General context and objectives of the project
More informationUnderstanding the Respiratory Microbiome of Commercial Poultry (Broilers)
Understanding the Respiratory Microbiome of Commercial Poultry (Broilers) Calvin L. Keeler, Jr. 1, Daniel Bautista 1, Cynthia Boettger 1, Thomas J. Carr, Jr. 2, Albert D Agostino 1, Sharon J. Keeler 1
More informationAvian Influenza: Outbreak in Spring 2015 and Preparing for Fall
Avian Influenza: Outbreak in Spring 2015 and reparing for Fall James A. Roth, DVM, hd Center for Food Security and ublic Health College of Veterinary Medicine Iowa State University Topics for Today Understanding
More informationInvestigation on the possible application of a serological DIVA monitoring strategy when a rhvt-h5 vaccine is used to control Avian Influenza
AVMA AAAP Convention 2016 August 7, San Antonio, Texas, USA Investigation on the possible application of a serological DIVA monitoring strategy when a rhvt-h5 vaccine is used to control Avian Influenza
More informationMutants and HBV vaccination. Dr. Ulus Salih Akarca Ege University, Izmir, Turkey
Mutants and HBV vaccination Dr. Ulus Salih Akarca Ege University, Izmir, Turkey Geographic Distribution of Chronic HBV Infection 400 million people are carrier of HBV Leading cause of cirrhosis and HCC
More informationSurveillance for Avian Influenza in the European Union in EU Reference Laboratory for Avian Influenza, APHA-Weybridge, UK
Surveillance for Avian Influenza in the European Union in 2016 EU Reference Laboratory for Avian Influenza, APHA-Weybridge, UK Overview 2016 surveillance programme in Poultry Wild birds Additional epidemiological
More informationESSENTIAL PROTECTION
ESSENTIAL PROTECTION for better life BROILER ND K VITABRON L VITAPEST L NEW L Supported by C H I C K P R O G R A M CONVENTIONAL vaccine range against Newcastle Disease CEVA HATCHERY I MMUNIZATION CONTROL
More informationMaximising the benefits of Serological Monitoring and Reporting
Maximising the benefits of Serological Monitoring and Reporting AviAfrica, 21-06-2017, Johannesburg Luuk Stooker, DVM Senior Product Manager BioChek Forecast on food Source: Rabobank Forecast on meat consumption
More informationE. coli peritonitis. Bernie Beckman, DVM Hy-Line International. Genetic Excellence. Genetic Excellence. Hy-Line International
E. coli peritonitis Bernie Beckman, DVM Hy-Line International Hy-Line International Genetic Excellence E. coli peritonitis Lesions Fibrin around the ova thin, yellow strings of fibrin that are very small
More informationUCD Veterinary Students in the Field
UCD Veterinary Students in the Field by Dr. Rodrigo Gallardo More than a dozen veterinary students this fall semester have visited poultry farms in California as part of their curriculum, and there are
More informationNewcastle disease. in the Czech Republic
Newcastle disease in the Czech Republic Petr Šatrán, Brussels, SCoPAFF 18. 4. 2018 Prevention of Newcastle Disease in the Czech Republic Vaccination against Newcastle disease Mandatory for: Registered
More informationOIE Situation Report for Avian Influenza
OIE Situation Report for Avian Influenza Latest update: 25/01/2018 The epidemiology of avian influenza is complex. The virus constantly evolves and the behavior of each new subtype (and strains within
More informationApplication of Reverse Genetics to Influenza Vaccine Development
NIAID Application of Reverse Genetics to Influenza Vaccine Development Kanta Subbarao Laboratory of Infectious Diseases NIAID, NIH Licensed Vaccines for Influenza Principle: Induction of a protective
More informationLaboratory Diagnosis of Avian Influenza and Newcastle Disease
Laboratory Diagnosis of Avian Influenza and Newcastle Disease Dennis A. Senne dennis.a.senne@aphis.usda.gov (515) 239-7551 U. S. Department of Agriculture, Animal and Plant Health Inspection Service, Veterinary
More informationVaccines of today and products needed for the short-, intermediate- and longterm. OIE/FAO OFFLU Conference Beijing China December 4-6, 2013
Vaccines of today and products needed for the short-, intermediate- and longterm XU, Wei-Cheng OIE/FAO OFFLU Conference Beijing China December 4-6, 2013 1 Influenza A reservoir & transmission H3N8, (H5N1)
More informationCommercial chicken vaccination: part 1 spray and aerosol usage
Vet Times The website for the veterinary profession https://www.vettimes.co.uk Commercial chicken vaccination: part 1 spray and aerosol usage Author : William Garton Categories : General, Vets Date : April
More informationPreparing for the Fall Flu Season. Jonathan Gubbay Medical Microbiologist Public Health Laboratory OAHPP
Preparing for the Fall Flu Season Laboratory Perspective Jonathan Gubbay Medical Microbiologist Public Health Laboratory OAHPP September 21, 2009 Objectives 1. Review the emergence of Novel Influenza A
More informationInfluenza B viruses are not divided into subtypes, but can be further broken down into different strains.
Influenza General Information Influenza (the flu) is a highly transmissible respiratory illness caused by influenza viruses. It can cause mild to severe illness, and may lead to death. Older people, young
More informationEnhancing animal health security and food safety in organic livestock production
Enhancing animal health security and food safety in organic livestock production Proceedings of the 3 rd SAFO Workshop 16-18 September 2004, Falenty, Poland Edited by M. Hovi, J. Zastawny and S. Padel
More informationGUMBORO FIELD EXPERIENCES IN SPAIN THINGS WE HAVE LEARNT!
GUMBORO FIELD EXPERIENCES IN SPAIN THINGS WE HAVE LEARNT! JOSÉ LUIS VALLS GARCIA Veterinario consultor avícola Barcelona, 15th March 2016 GUMBORO is an Enzootic disease : Challenge will come immediately
More informationRT-PCR. variant 2 variant 2 H120. :
RT- * RT- MWG H120 RT- azad.rahimi@gmail.com : * Cavanagh. (Iran//19/08) Hosseini QX Najafi (Iran/QX/H179/11) g PBS IR1 mass IS/720 QX Hashemzadeh RT- RNase SX1 RT- SX2 Nested- RT- SX3 SX3 Denaturation)
More informationInfluenza. Gwen Clutario, Terry Chhour, Karen Lee
Influenza Gwen Clutario, Terry Chhour, Karen Lee Overview Commonly referred to as the flu Defined as a highly contagious viral infection where it starts at the upper respiratory tract and attacks the nose,
More informationTRUSTWORTHY POULTRY CEVAC CORYMUNE RANGE. Unique combination vaccines to protect against Coryza and Salmonella. Supported by CEVAC CORYMUNE 4K
CEVAC CORYMUNE RANGE Unique combination vaccines to protect against Coryza and Salmonella TRUSTWORTHY POULTRY CEVAC CORYMUNE RANGE Supported by CEVAC CORYMUNE 4K Unique broad combination vaccine against
More informationHIV and drug resistance Simon Collins UK-CAB 1 May 2009
HIV and drug resistance Simon Collins UK-CAB 1 May 2009 slides: thanks to Prof Clive Loveday, Intl. Clinical Virology Centre www.icvc.org.uk Tip of the iceberg = HIV result, CD4, VL Introduction: resistance
More informationSUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION Viral vector vaccines expressing nucleoprotein and phosphoprotein genes of avian bornaviruses ameliorate homologous challenge infections in cockatiels and common canaries Marita
More informationJ. Callahan, Pabilonia, K.L.. Presented by Nardy Robben. The world leader in serving science
The effect of pooling 5 or 11 Tracheal/Oropharyngeal (TR/OP) swabs on the performance of a USDA Licensed reverse transcriptase, real-time PCR (rrt-pcr) test for avian influenza virus J. Callahan, Pabilonia,
More information