Dr.Christophe Cazaban
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1 Dr.Christophe Cazaban Technical Manager Biology Innovation Strategy Department. Ceva Sante Animale, France Graduated from the French Veterinary University of Lyon in 1993 He completed his education with specific training on virology, immunology and avian pathology 2002 : Joined Ceva as an international technical manager, 4 years later he was manager for Turkey, Middle East and Africa 2010 Present : Technical Manager Biological Innovation Strategy Department Topic : Modern Strategy for Protection of Gumboro Disease in Asia
2 GUMBORO DISEASE Modern strategy for protection in Asia Hatchery Vaccination Summit Jeju, Korea, April 26-27th 27th 2012 Christophe CAZABAN
3 WHY A MODERN STRATEGY? 1.IS IBD STILL PRESENT IN THE FIELD?
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7 WHY A MODERN STRATEGY? 1.IS IBD STILL PRESENT IN THE FIELD? IBD (vv FORM) IS STILL PRESENT IN ASIA
8 WHY A MODERN STRATEGY? 1.IS IBD STILL PRESENT IN THE FIELD? 2.IS IBD CONTROL WORTHWHILE?
9 vvibd in broilers: Flensburg (cited by de Wit, 2001) Mortality + condemnations + vaccination costs 0.33 USD / chicken
10 WHY A MODERN STRATEGY? 1.IS IBD STILL PRESENT IN THE FIELD? 2.IS IBD CONTROL WORTHWHILE? IBD IS QUITE COSTLY.
11 VACCINES AND VACCINATIONS
12 Types of vaccines: Live attenuated vaccines mild intermediate intermediate t plus DW in the field
13 SWITCH FROM FARM TO HATCHERY
14 Types of vaccines: Live attenuated vaccines mild intermediate intermediate plus Vectored vaccines rhvt-ibd in ovo or day-old in the hatchery Immune-complex vaccines Cevac Transmune in ovo or day-old in the hatchery DW in the field
15 IBDV ELISA Titers at slaughter time (one year) Transmune Ene2009 Feb2010 Ecat Company B: Hatchery vaccine (In Ovo) Biocheck 51D 60D 53D 41D 56D 55D 45D 52D 54D 47D 43D 50D 47D 47D 53D 42D 44D 51D 41D 47D 45D 43D 42D 44D 41D 44D 50D 43D 44D 40D 44D 47D Resto Company B: Drinking Water (Biocheck) 53D 53D 55D 52D 80D 54D 55D 53D 53D 41D 52D 56D 60D 56D 51D 47D 53D 53D 57D 52D 55D 52D 42D
16 SWITCH FROM FARM TO HATCHERY? Targetting: 100% administration quality; Easier checks and audits; Higher vaccination consistency; Less stress to the chicks; Earliest immunization; i Access to MDA-insensitive vaccines; Farmers focussing on farm management.
17 Types of vaccines: Live attenuated vaccines mild intermediate intermediate plus Vectored vaccines rhvt-ibd in ovo or day-old in the hatchery Immune-complex vaccines Cevac Transmune in ovo or day-old in the hatchery DW in the field
18 CEVAC TRANSMUNE IBD: what is it? Winterfield Specific Antibodies Vaccine virus (Virus Protecting Factor) IBDV
19 ELISA (2 log) HIGH LEVEL LOW LEVEL THE VACCINE REPLICATES : - WHATEVER THE MATERNAL ANTIBODY TITRE AT DAY OLD - WHATEVER THE HOMOGENEITY OF THE FLOCK Age (d.)
20 IBDV ELISA Titers at slaughter time (one year) Transmune Ene2009 Feb2010 Ecat Company B: Hatchery, immune complex vaccine (In Ovo) Biocheck 51D 60D 53D 41D 56D 55D 45D 52D 54D 47D 43D 50D 47D 47D 53D 42D 44D 51D 41D 47D 45D 43D 42D 44D 41D 44D 50D 43D 44D 40D 44D 47D Resto Company B: Drinking Water (Biocheck) 53D 53D 55D 52D 80D 54D 55D 53D 53D 41D 52D 56D 60D 56D 51D 47D 53D 53D 57D 52D 55D 52D 42D
21 Field experience: THE NETHERLANDS (Swart W., 2009)
22 Semi-integration, i ti 50, Cobb broilers processed a day (37-38 days); Houses of 15,000 birds each; 3 cycles; 6 houses: immune-complex vaccine sc route (Dovac, independent hatchery); 4 houses: controls (routine: 1 intermediate D78 vaccine in the farm, DW, 16 days of age); IBD Idexx ELISA (GD Deventer) RT-PCR on bursa samples (Ceva Phylaxia, 30 bursas / date)
23 Hatchery Immune complex vaccine Field Drinking water routine vaccine GMT Seropositivity rate GMT Seropositivity rate Round % % Round d % % Round % %
24 VACCINE TAKE MONITORING BY PCR FROM BURSA OF FABRICIUS Historical controls D28 33% D35 67% D28 Transmune Controls R1 100% 0% R2 83% 75% R3 100% 5%
25 CONCLUSION Hatchery, immune-complex vaccine: Strong, robust, early and consistent vaccine take cycle after cycle; Field, drinking water vaccine: erratic vaccine take cycle after cycle.
26 VECTOR VACCINES VP2 IBDV IBDV = Donor IBDV Genome rhvt- VP2 VP2 gene HVT HVT Insertion site HVT = Vector Inserted VP2 gene
27 ASSETS of a rhvt-ibd vaccine: No live whole Gumboro strain; Cell-associated (frozen) HVT vector = no interference with MDA; Administration at hatchery (sc or in ovo); No colonization i of the bursa by the vaccine.
28 DRAWBACKS of a rhvt-ibd vaccine
29 vvibdv Challenges
30 Protection Max. Superiority area of the immune complex vaccine Immune complex Vectored HVT IBD Min. Maternally derived antibodies 3 weeks 6 weeks Age
31 Two different approaches: - Immune Complex : 100% protection as early as needed; - Vectored HVT-IBD : slow and incomplete protection when requested.
32 TO SUMMARIZE
33 Types of vaccines: Immune-complex vaccines Live attenuated vaccines mild intermediate intermediate plus DW in the field Vectored vaccines rhvt-ibd in ovo or day-old in the hatchery Cevac Transmune in ovo or day-old in the hatchery
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