CevaC Mass L THe Ceva Mass solution
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1 CevaC Mass L THe Ceva Mass solution Ceva Santé Animale S.A. - 10, av. de la Ballastière - BP Libourne Cedex - France Tel : Fax :
2 CEVAC MASS L is a live attenuated vaccine (strain B48) belonging to the Massachusetts serotype of Infectious Bronchitis Virus (IBV) CEVAC MASS L is a freeze-dried vaccine available in glass vials of 1000, 2500 and 5000 dose presentations.
3 CEVAC MASS L A unique IBV MASSAChuSETTS STRAIN CEVAC MASS L is a Massachusetts IBV, strain B48. This strain differs from H120 and Ma5 vaccine strains. The S1 region of the genome was amplified by RT-PCR and was fully sequenced. The Genbank H120 and Ma5 are nearly identical (>99.5%, from this group), the B48 strain is clearly different, with only about 98% identity compared to the other Mass-type vaccine strains. Seq-> h120gb Ma5 B48c M41 H120Gb 1,000 0,996 0,978 0,975 Ma5 1,000 0,978 0,975 B48c 1,000 0,980 M41 1,000 Comparison between H120 and B48 strains (1) 3 groups of 48 SPF chickens were vaccinated at day one by Eye drop route and challenged at 23 days of age with 10 3 EID 50 per bird with a M41 IBV strain. Ciliostastis score CEVAC BRoN L CEVAC MASS L 3 dpc dpc dpc 0 0 % of virus recovery post challenge 100 % virus recovery control Cevac Bron 120L Cevac Mass L 0 3Days 5Days 7Days CEVAC MASS L offered the best protection evaluated by ciliostasis score and virus recovery after challenge compare to the h120 vaccine strain.
4 A STRoNg PRoTECTIoN from 3 weeks until 6 weeks of AgE Challenge laboratory trials in commercial broilers have been conducted at 3 weeks and 6 weeks of age after spray vaccination with CEVAC MASS L at day of age. Challenge virus was the M41 Massachusetts virus applied by eye drop at 3.5 log 10 EID 50 /bird. Ciliostatis score 5 days post challenge weeks score weeks 0 CEVAC MASS L control Ciliostasis score post-challenge after M41 virus challenge (DV ) day one application to commercial broilers demonstrated onset of immunity after M41 challenge at 3 weeks. The latest challenge was at 6 weeks of age with evident protection. A STRoNg REduCTIoN of ChALLENgE VIRuS ExCRETIoN After challenge at 3 & 6 weeks of age, infectious bronchitis challenge virus load in the trachea was evaluated by qpcr. mean IBV copy numbers per trachea samples copy numbers/ml (log10) CEVAC MASS L control 3 weeks 6 weeks day one application to commercial broilers of CEVAC MASS L was able to dramatically reduce the challenge virus load in the trachea after an IBV M41 challenge.
5 VACCINATIoN PRogRAM Multiple Infectious bronchitis viruses are present all other the world and most of the time several viruses are circulating in a country at the same time. Your vaccination program should be able to induce a broad protection in your chicks. In order to evaluate protection induced by the combination of CEVAC MASS L and CEVAC IBird several studies have been conducted with recent IBV isolates from different parts of the world. Challenge strain Vaccination program CEVAC IBird + CEVAC MASS day B Mass QX Q1 J-2 Malaysian nephropathogenic Variant 2 QX from Asia or Europe, Q1from Latin America, variant 2 and J-2 from Middle East (2) CEVAC MASS L and CEVAC IBird combined in the same vaccination program were able to induce a strong protection against these recent field IBV isolates.
6 CevaC Mass L THe Ceva Mass solution Possible combination with CEVAC IBird in the vaccination program Broad Protection : Mass, 793 B, Qx, Q1, J2, Malaysian variant No need of IBV booster during rearing Simplification of the vaccination program Desvac equipment and CHICK Program Better control of vaccine application References 1. PICAULT Jean-Paul 1, LAMANDÉ Josiane 1, ALLÉE Chantal 1, MORIN Yannick 2 and COMTE Sylvain; Efficay study with 3 attenuated infectious bronchitis vaccine strains of the Massachusetts type in SPF chickens. XIII Congress of the World Veterinary Poultry Association, July 19-23, 2003, Denver, Colorado. 2. T Tatar-Kis, Walkone Kovacs E., Felfoldi A., Nagy Z, Kiss I, Mato T, Palya V. Portection achieved by a vaccination programme comprising Mass and 793 B type vaccines against recent variant IBV strains circulating in the Middle East. VII Symposium on avian corona-& pneumovirus. Rauischholzhausen, Germany, june 2012, Summary Product Characteristic Composition CEVAC MASS L lyophilisate for oculonasal suspension for chicken. Indications For the active immunisation of chickens against infectious bronchitis (Massachusetts serotype), in order to reduce respiratory clinical signs, detrimental effect on the ciliary activity and presence of virus in the trachea. Protection was demonstrated by challenge with the Massachusetts M41 strain. Onset of immunity: 3 weeks following vaccination. Duration of immunity: 6 weeks following vaccination. Administration For nebulisation use. The vaccine should be administered from one day of age, one dose / chicken. Dilute the vaccine in distilled water, or in cold, clear water, free from disinfectants. The quantity of water should be sufficient to allow a uniform distribution of the vaccine when spraying the chickens. The 1000 dose vaccine is recommended to be dissolved in 200 ml water, whereas this ratio should be considered when dissolving other types of presentation. Vaccination is recommended in the form of coarse spray assuring a droplet size of µm. It is preferable that the chickens are sitting together in dim light or closely confined during spraying. The ventilation should be switched off during and after vaccination in order to avoid turbulences. Vaccination should be performed during the coolest time of the day. Special precautions for use Vaccinate healthy susceptible birds only. The safety of the veterinary medicinal product has not been studied during lay. Do not use in birds in lay and within 4 weeks before the onset of the laying period. CEVA SANTE ANIMALE , Avenue de la Ballastière - BP LIBOURNE Cedex (France) Tel : Fax :
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