16: Proteins & Genetics
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1 16: Proteins & Genetics Reminders: 1. Due in lab this week: a. Pre- lab for Lab 07 b. No lab report J 2. Exam 6 Monday (see SPOC for details) Last names A F Ryan Lounge M Last names G- Z here (1 pt for right room)
2 When we say a mutation replaces leu222 with ser what does that mean? A. The protein is first made with leu at position 222 and the mutation causes amino acid 222 to be removed and replaced with ser. B. The protein is first made with leu at position 222 and the mutation causes the side chain of leu222 to be rearranged into the side chain of ser. C. The mutation causes the protein to be made with ser at position 222 instead of leu.
3 Considering that leu222 is in the interior of the normal protein, why would a mutation that replaces it with serine lead to an inactive protein? A. The side chain of serine is hydrophobic and would therefore not remain in the hydrophilic core of the protein, thus disrupting the protein s shape. B. The side chain of serine is hydrophilic and would therefore not remain in the hydrophobic core of the protein, thus disrupting the protein s shape. C. Any change in amino acid sequence will disrupt the shape of a protein. D. Serine will form hydrogen bonds with the other amino acids in the hydrophobic core, disrupting the shape of the protein.
4 Which amounts of each of the two forms of the PAH protein would be found in the cells of an individual with genotype AB? A. 100% functional 0% non- functional B. 0% functional 100% non- functional C. 50% functional 0% non- functional D. 50% functional 50% non- functional E. 75% functional 25% non- functional
5 What would be the phenotype of an individual with genotype AB? (listed in increasing severity) A. Fully normal (same as AA) B. Mild PKU C. 1/2 PKU half as bad as BB D. Strong PKU E. Full PKU (same as BB)
6 Which is correct? A. The normal phenotype of the A allele is dominant to the PKU phenotype of the B allele B. The normal phenotype of the A allele is recessive to the PKU phenotype of the B allele C. The PKU/normal phenotypes of the A and B alleles are co- dominant. D. The PKU/normal phenotypes of the A and B alleles are incompletely dominant. E. More than one of the above
7 Which amounts of the two forms of the PRNP protein would be found in the brain cells of a P P* individual? A. 100% normal 0% mis- folded B. 0% normal 100% mis- folded C. 50% normal 0% mis- folded D. 50% normal 50% mis- folded E. 25% normal 75% mis- folded
8 What would be the phenotype of an individual with genotype P P* and what can we conclude about the FFI phenotype of the P* allele? A. Normal; therefore, the FFI phenotype of P* is dominant B. Normal; therefore, the FFI phenotype of P* is recessive C. FFI; therefore, the FFI phenotype of P* is dominant D. FFI; therefore, the FFI phenotype of P* is recessive E. More than one of the above.
9 Which amounts of which forms of the lipo- polysaccharide would be found on an individual with genotype I A i? A. 100% O 0% A B. in between A and C C. 50% O 50% A D. in between C and E E. 0% O 100% A
10 Which amounts of which forms of the lipo- polysaccharide would be found on an individual with genotype I A I B? A. 100% A 0% B B. in between A and C C. 50% A 50% B D. in between C and E E. 0% A 100% B
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