High Strength and Hydrophilic Chitosan Microspheres for The Selective Enrichment of N-Glycopeptides

Transcription

1 Supporting Information for High Strength and Hydrophilic Chitosan Microspheres for The Selective Enrichment of N-Glycopeptides Xiao-Mei He, 1 Xi-Chao Liang, 2 Xi Chen, 3 Bi-Feng Yuan, 1 Ping Zhou, 2 Li-Na Zhang,* 2 Yu-Qi Feng* 1 1 Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan , P.R. China 2 College of Chemistry and Molecular Sciences, Wuhan University, Wuhan , P.R. China. 3 Wuhan Institute of Biotechnology, Wuhan , P.R. China. These authors contributed equally to this work Corresponding author: * Yu-Qi Feng, Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan , P.R. China. Tel: ; Fax: ; yqfeng@whu.edu.cn * Li-Na Zhang, Department of Chemistry, Wuhan University, Wuhan , P.R. China. Tel: ; Fax: ; zhangln@whu.edu.cn S-1

2 Contents: 1. Experiment section Sample preparation Mass spectrometry analysis Data Analysis 2. Supporting figures 3. Supporting tables S-2

3 Experimental Section Sample preparation Horseradish peroxidase (HRP) (1 mg) and immunoglobulin G (IgG) were dissolved in NH 4 HCO 3 solution (50 mm, 1 ml), respectively, and denatured at 95 o C for 5 min. To the above solution, trypsin (40 µg) was added and the mixture was incubated at 37 o C for overnight. The obtained HRP digests and IgG digests were stored at -20 o C until use. Bovine serum albumin (BSA, 1 mg), chicken avidin (1 mg) or bovin fetuin (1 mg) was dissolved in 200 µl of denaturing buffer solution (8 M urea, 0.1 M Tris, 4 mm CaCl 2, ph 8.5). The protein solution incubated with dithiothreitol (10 mm) for 20 min at 37 o C to reduce protein disulfide bonding. Iodoacetamide (20 mm) was added to the solution and incubated for an additional 30 min at 37 o C in dark. The reduced and alkylated protein mixture was diluted four times with Tris-HCl buffer (0.1 M, ph 8.5), and the mixture was digested with trypsin at an enzyme to substrate ratio of 1: 50 (w/w) by incubating at 37 o C for overnight. The obtained protrin digests were lyophilized to dryness and stored at -20 C until use. The protein concentration of serum sample was measured using bicinchoninic acid method. Proteins in human serum (10 µl) were precipitated with some precipitant (50% acetone, 49.9% ethanol, 0.1% acetic acid, v/v/v) at 4ºC for 3 h, and then centrifuged at 4,000 rpm under 4ºC for 30 min. The precipitate was re-suspended in Tris-HCl buffer (8 M urea, 0.1 M Tris, 4 mm CaCl 2, ph 8.5) to make the final concentration of protein at 5 mg/ml. 1 The following digestion procedure was S-3

4 the same as that of BSA, and the digested products were desalted by C18 cartridge and stored at -20ºC until use. Sprague dawley (SD) male rat (about 220 g in weight), purchased from Hubei provincial center for disease control and prevention (Wuhan, Hubei, China), was sacrificed and the brain was quickly excised. After removing blood with ice-cold physiological saline, the brain tissue was minced and homogenized in a homogenizer (Pottter-Elvejhem) with a Teflon piston on ice for 20 times, using 8 ml of RIPA lysis buffer (P0013D, Beyotime Biotechnology) per 1 g of tissue. The suspension was then vortexed on ice for 30 min followed by centrifugation at 15,000 rpm under 4ºC for 30 min. The protein concentration of the supernatant was measured using bicinchoninic acid method. Proteins were precipitated with some precipitant (50% acetone, 49.9% ethanol, 0.1% acetic acid, v/v/v) at 4ºC for 3 h, and then centrifuged at 4,000 rpm under 4ºC for 30 min. The precipitate was re-suspended in Tris-HCl buffer (8 M urea, 0.1 M Tris, 4 mm CaCl 2, ph 8.5) to make the final concentration of protein at 5 mg/ml. 1 The following digestion procedure was the same as that of BSA, and the digested products were desalted by C18 cartridge and stored at -20ºC until use. Mass spectrometry analysis All matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) spectra were recorded in positive ion mode on a 5800 Proteomics Analyzer (Applied Biosystems, Framingham, MA, USA) with the Nd-YAG laser at 383 nm, a repetition rate of 200 Hz and an acceleration voltage of 20 kv. Tandem S-4

5 mass spectra of some glycopeptides were obtained using MALDI-TOF/TOF MS. ExPASY compute pi/mw tool was used to calculate the theoretical m/z of peptides ( GlycoMod tool ( was used to predict possible glycan structures based on the experimentally determined masses of glycopeptides combined with their individual tandem mass spectra. For the analysis of intact glycopeptides from bovine fetuin digests, the TripleTOF mass spectrometry (ABSciex) equipped with a nanospray was used. The MS analysis was carried out under positive ion mode with a nanospray voltage at 2.3 kv. Full scan MS data were obtained at m/z And information-dependent acquisition (IDA) mode was employed with the top 15 precursors subjected to MS/MS analysis. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used to analyze the samples from human serum and rat brain, and the analysis was carried out according to the previous work. 1 The enriched glycopeptides from human serum and rat brain were treated with PNGase F (an endoglycosidic enzyme). The de-glycan glycopeptides were separated and identified on TripleTOF equipped with a nanospray source. Peptides were first loaded onto a C18 trap column (5 mm 0.3 mm i.d., 5 µm, Agilent Technologies) and theneluted into a C18 analytical column (150 mm 75 µm i.d., 3 µm, 100 Å, Eksigent). Mobile phase A (3% DMSO, 97% H 2 O, 0.1% formic acid) and mobile phase B (3% DMSO, 97% ACN, 0.1% formic acid) were used to establish a 100 min S-5

6 gradient, which was comprised of 0 min in 5% B, 65 min of 5-23% B, 20 min of 23-52% B, 1 min of 52-80% B, 4 min of 80% B, 0.1 min of 80-5% B, and 10 min of 5% B. A constant flow rate was set at 300 nl/min. MS scans were conducted from 350 to 1500 amu, with a 250 ms time span. For MS/MS analysis, each scan cycle consisted of one full-scan mass spectrum (with m/z ranging from 350 to 1500 and charge states from 2 to 5) followed by 40 MS/MS events. The threshold count was set to 120 to activate MS/MS accumulation and former target ion exclusion was set for 18 s. Data Analysis Raw data from TripleTOF were analyzed with ProteinPilot Software 5.0. Data were searched against the Uniprot human reference proteome database (version ) and Uniprot rat reference proteome database (version ) using the following parameters: trypsin was specified as digesting enzyme; up to two missing cleavages of trypsin were allowed; cysteine carboxamidomethylation (C) was set as a fixed modification, oxidation on methionine (M) and deamidation (NQ) were set as the variable modifications. Search effort was set to rapid ID. A 95% confidence in Protein Pilot was selected to calculate the number of identification. S-6

7 Figure S1. Solid-state 13 C NMR spectra of CS powder and CSMs. S-7

8 Figure S2. Potentiometric titration of CS powder and CSMs (a); titration curves and two inflexion points of CS powder and CSMs (b). S-8

9 Figure S3. Photograph of compressive test of the CSMs with the FT-IR Infrared compression mold (a) and schematic image of the compression test process (b). S-9

10 Figure S4. FT-IR spectrum (a) and XRD pattern (b) of CSMs. S-10

11 Figure S5. MALDI-TOF/TOF tandem mass spectra of four representative N-glycopeptides at m/z= 1843 (a), 2612 (b), 3352 (c) and 3673 (d) from HRP. Blue square, N-acetylglucosamine residue; red circle, mannose residue; yellow circle, xylose residue; green triangle, fucose residue. S-11

12 Figure S6. MALDI mass spectra of (a) HRP digests and (b) the tryptic digest mixtures of HRP and BSA (HRP: BSA= 1: 10) with the Sepharose CL-6B enrichment. Glycopeptides are marked with Hn. S-12

13 Figure S7. MALDI mass spectra of tryptic digest of HRP after enrichment with the CSMs. The concentrations of HRP digests used in the experiments were M (a) and M (b). Glycopeptides are marked with Hn. S-13

14 Figure S8. MALDI-TOF/TOF tandem mass spectra of four representative N-glycopeptides at m/z= 3052 (a), 3377 (b), 3411 (c) and 3459 (d) from avidin. Blue square, N-acetylglucosamine residue; red circle, hexose residue; green triangle, fucose residue. S-14

15 Figure S9. MALDI-TOF/TOF tandem mass spectra of four representative N-glycopeptides at m/z= 2602 (a), 2634 (b), 2764 (c) and 2926 (d) from IgG. Blue square, N-acetylglucosamine residue; red circle, hexose residue; green triangle, fucose residue. S-15

16 Figure S10. The overlap of MALDI-TOF/TOF tandem mass spectra of two deglycosylated peptides from the N-glycopeptides of avidin in the m/z ranges of (a) and (b). S-16

17 Figure S11. ESI-MS/MS tandem mass spectra of four representative N-glycopeptides at m/z= (5+) (a), (4+) (b), (4+) (c) and (4+) (d) from fetuin. Blue square, N-acetylglucosamine residue; red circle, hexose residue; purple diamond, sialic acid residue. S-17

18 Figure S12. The MS intensities of six selected glycopeptides from tryptic digests of human IgG (3 µg) after enrichment with different amounts of CSMs. S-18

19 Table S1. Oligosaccharide composition and the sequences of the N-glycopeptides detected from HRP digests. Peak Observed numbers m/z Oligosaccharides Amino acid sequences a H XylMan 3 GlcNAc 2 Fuc NVGLN#R H XylMan 3 GlcNAc 2 Fuc N#TTSFR H XylMan 3 GlcNAc 2 Fuc PNVSN#IVR H XylMan 3 GlcNAc 2 Fuc ASILLDN#TTSFR H GlcNAc 2 Fuc SFAN#STQTFFNAFVEAMDR H XylMan 3 GlcNAc 2 Fuc SSPN#DTIPLVR H XylMan 3 GlcNAc 2 Fuc PTLN#TTYLQTLR H XylMan 3 GlcNAc 2 MGN#ITPLTGTQGQIR H Man 2 GlcNAc 2 MGN#ITPLTGTQGQIRLNCR H GlcNAcFuc GLIQSDQELFSSPN#DTIPLVR H GlcNAcFuc LHFHDCFVNGCDASILLDN#TTSFR H XylMan 3 GlcNAc 2 Fuc GLCPLNGN#LSALVDFDLR H XylMan 3 GlcNAc 2 SFAN#STQTFFNAFVEAMDR H XylMan 3 GlcNAc 2 Fuc QLTPTFYDNSCPN#VSNIVR H XylMan 3 GlcNAc 2 Fuc SFAN#STQTFFNAFVEAMDR H XylMan 3 GlcNAc 2 GLIQSDQELFSSPN#DTIPLVR H XylMan 3 GlcNAc 2 Fuc NQCRGLCPLNGN#LSALVDFDLR H XylMan 3 GlcNAc 2 Fuc GLIQSDQELFSSPN#DTIPLVR H Man 2 GlcNAc 2 LHFHDCFVNGCDASILLDN#TTSFRTEK H XylMan 3 GlcNAc 2 Fuc LHFHDCFVNGCDASILLDN#TTSFR H XylMan 4 GlcNAc 2 Fuc QLTPTFYDNSC(AAVESACPR)PN#VSNIVR H XylMan 3 GlcNAc 2 Fuc QLTPTFYDNSC(AAVESACPR)PN#VSNIVR H XylMan 3 GlcNAc 2 LYN#FSNTGLPDPTLN#TTYLQTLR H XylMan 3 GlcNAc 2 Fuc LYN#FSNTGLPDPTLN#TTYLQTLR a The N-glycosylation sites are marked with N#. GlcNAc=N-acetylglucosamine, Fuc=fuctose, Man=mannose, Xyl=xylose. The theoretical amino acid sequences and oligosaccharides were cited from previous works. 2-8 S-19

20 Table S2. Oligosaccharide composition and the sequences of the N-glycopeptides detected from chicken avidin digests. Peak Observed numbers m/z Oligosaccharides Amino acid sequences A HexNAc WTNDLGSN#(M-48)TIGAVNSR A HexNAc WTNDLGSN#MTIGAVNSR A HexNAc 2 WTNDLGSN#(M-48)TIGAVNSR A Hex 2 HexNAc 2 WTNDLGSN#MTIGAVNSR A Hex 3 HexNAc 2 WTNDLGSN#MTIGAVNSR A Hex 4 HexNAc 2 WTNDLGSN#(M-48)TIGAVNSR A Hex 4 HexNAc 2 WTNDLGSN#MTIGAVNSR A Hex3HexNAc 3 WTNDLGSN#MTIGAVNSR A Hex 5 HexNAc 2 WTNDLGSN#(M-48)TIGAVNSR A Hex 5 HexNAc 2 WTNDLGSN#MTIGAVNSR A Hex 4 HexNAc 3 WTNDLGSN#MTIGAVNSR A Hex 6 HexNAc 2 WTNDLGSN#(M-48)TIGAVNSR A Hex 6 HexNAc 2 WTNDLGSN#MTIGAVNSR A Hex 5 HexNAc 3 WTNDLGSN#MTIGAVNSR A Hex 4 HexNAc 4 WTNDLGSN#MTIGAVNSR A Hex 7 HexNAc 2 WTNDLGSN#(M-48)TIGAVNSR A Hex 7 HexNAc 2 WTNDLGSN#MTIGAVNSR A Hex 5 HexNAc 4 WTNDLGSN#(M-48)TIGAVNSR A Hex 5 HexNAc 4 WTNDLGSN#MTIGAVNSR A Hex 4 HexNAc 5 WTNDLGSN#MTIGAVNSR A Hex 6 HexNAc 4 WTNDLGSN#(M-48)TIGAVNSR A Hex 6 HexNAc 4 WTNDLGSN#MTIGAVNSR A Hex 5 HexNAc 5 WTNDLGSN#MTIGAVNSR a The N-glycosylation sites are marked with N#. Hex= hexose, HexNAc= N-acetylhexosamine. The theoretical amino acid sequences and oligosaccharides were cited from the previous works 9,10. S-20

21 Table S3. Oligosaccharide composition and the sequences of the N-glycopeptides detected from human IgG digests. Peak Observed number m/z Oligosaccharides Amino acid sequences I Hex 3 HexNAc 3 Fuc EEQFN#STFR I Hex 3 HexNAc 3 Fuc EEQYN#STYR I Hex 3 HexNAc 4 EEQFN#STFR I Hex 3 HexNAc 4 EEQYN#STYR I Hex 4 HexNAc 3 Fuc EEQFN#STFR I Hex 3 HexNAc 4 Fuc EEQFN#STFR I Hex 4 HexNAc 4 EEQFN#STFR I Hex 3 HexNAc 4 Fuc EEQYN#STYR I Hex 4 HexNAc 4 EEQYN#STYR I Hex 3 HexNAc 5 EEQYN#STYR I Hex 4 HexNAc 4 Fuc EEQFN#STFR I Hex 5 HexNAc 4 EEQFN#STFR I Hex 4 HexNAc 4 Fuc EEQYN#STYR I Hex 3 HexNAc 5 Fuc EEQFN#STFR I Hex 4 HexNAc 5 EEQFN#STFR I Hex 3 HexNAc 5 Fuc 1 EEQYN#STYR I Hex 4 HexNAc 5 EEQYN#STYR I Hex 5 HexNAc 4 Fuc EEQFN#STFR I Hex 5 HexNAc 4 Fuc EEQYN#STYR I Hex 4 HexNAc 5 Fuc EEQFN#STFR I Hex 5 HexNAc 5 EEQFN#STFR I Hex 4 HexNAc 5 Fuc EEQYN#STYR I Hex5HexNAc5Fuc EEQFN#STFR I Hex 5 HexNAc 5 Fuc EEQYN#STYR a The N-glycosylation sites are marked with N#. Hex= hexose, HexNAc= N-acetylhexosamine, Fuc=fuctose. The theoretical amino acid sequences and oligosaccharides were cited from the previous works 2,9,11. S-21

22 Table S4. The recovery yields of selected glycopeptides from chicken avidin and human IgG digests using CSMs. Peak numbers Observed m/z Oligosaccharides a Glycanforms Recovery % A high-mannose 84.1±7.8 A high-mannose 80.3±5.4 A hybrid-type 82.2±4.8 A hybrid-type 93.4±13 A high-mannose 83.3±9.8 A hybrid-type 90±5.9 I complex-type 89.1±3.0 I complex-type 91.3±12.1 I complex-type 84.6±6.3 I complex-type 88.6±8.1 I complex-type 91.7±9.3 I complex-type 96±10.2 a Blue square, N-acetylglucosamine residue; red circle, monnose residue; yellow circle, galactose residue; green triangle, fucose residue. S-22

23 Table S5. Oligosaccharide composition and the sequences of the N-glycopeptides detected from fetuin digests. Peak numbers Theoretical mass a (Da) Experimental m/z Charge Oligosaccharides a Amino acid sequences a F NeuAc 2 Hex 6 HexNAc 5 KLCPDCPLLAPLN#DSR F NeuAc 3 Hex 6 HexNAc 5 KLCPDCPLLAPLN#DSR F NeuAc 4 Hex 6 HexNAc 5 KLCPDCPLLAPLN#DSR F NeuAcHex 5 HexNAc 4 KLCPDCPLLAPLN#DSR F NeuAc 2 Hex 5 HexNAc 4 KLCPDCPLLAPLN#DSR F NeuAc 2 Hex 6 HexNAc 5 KLCPDCPLLAPLN#DSR F NeuAc 3 Hex 6 HexNAc 5 KLCPDCPLLAPLN#DSR F NeuAc 4 Hex 6 HexNAc 5 KLCPDCPLLAPLN#DSR F NeuAc 2 Hex 5 HexNAc 4 KLCPDCPLLAPLN#DSR F NeuAc 3 Hex 6 HexNAc 5 LCPDCPLLAPLN#DSR F NeuAcHex 5 HexNAc 4 LCPDCPLLAPLN#DSR F NeuAc 4 Hex 6 HexNAc 5 LCPDCPLLAPLN#DSR F NeuAc 2 Hex 6 HexNAc 5 LCPDCPLLAPLN#DSR F NeuAcHex 5 HexNAc 4 LCPDCPLLAPLN#DSR F NeuAcHex6HexNAc 5 LCPDCPLLAPLN#DSR F NeuAc 2 Hex 6 HexNAc 5 LCPDCPLLAPLN#DSR F NeuAcHex 6 HexNAc 5 LCPDCPLLAPLN#DSR F NeuAc 3 Hex 5 HexNAc 6 F NeuAc 4 Hex 7 HexNAc 6 F NeuAc 4 Hex 7 HexNAc 6 F NeuAc 4 Hex 7 HexNAc 6 F NeuAc 2 Hex 6 HexNAc 5 F NeuAc 3 Hex 6 HexNAc 5 RPTGEVYDIEIDTLETTC HVLDPTPLAN#CSVR VVHAVEVALFNAES N#GSY VVHAVEVALFNAES N#GSY VVHAVEVALFNAES N#GSYLQLVEISR VVHAVEVALFNAES N#GSYLQLVEISR VVHAVEVALFNAES N#GSYLQLVEISR S-23

24 F NeuAc 2 Hex 7 HexNAc 6 F NeuAc 3 Hex 7 HexNAc 6 F NeuAc 5 Hex 7 HexNAc 6 F NeuAc 2 Hex 6 HexNAc 5 F NeuAc 3 Hex 6 HexNAc 5 F NeuAc 2 Hex 7 HexNAc 6 F NeuAc 3 Hex 7 HexNAc 6 F NeuAc 5 Hex 7 HexNAc 6 F NeuAc 3 Hex 7 HexNAc 6 F NeuAcHex 6 HexNAc 5 F NeuAc 2 Hex 7 HexNAc 7 F uc F NeuAc 3 Hex 7 HexNAc 7 F uc VVHAVEVALFNAES N#GSYLQLVEISR VVHAVEVALFNAES N#GSYLQLVEISR VVHAVEVALFNAES N#GSYLQLVEISR VVHAVEVALFNAES N#GSYLQLVEISR VVHAVEVALFNAES N#GSYLQLVEISR VVHAVEVALFNAES N#GSYLQLVEISR VVHAVEVALFNAES N#GSYLQLVEISR VVHAVEVALFNAES N#GSYLQLVEISR VVHAVEVALFNAES N#GSYLQLVEISR VVHAVEVALFNAES N#GSYLQLVEISR VVHAVEVALFNAES N#GSYLQLVEISR VVHAVEVALFNAES N#GSYLQLVEISR a The N-glycosylation sites are marked with N#. Hex= hexose, HexNAc= N-acetylhexosamine, NeuAc= sialic acid, Fuc=fuctose. The theoretical mass, amino acid sequences and oligosaccharides were cited from the previous works S-24

25 Table S6. Detailed information of the observed N-glycopeptides obtained from tryptic digests of human serum. Data were searched against the Uniprot human reference proteome database. In the Accessions and Names columns, the proteins in a Protein Group were listed together, and the residue numbers of N-glycosylation sites on the first protein of each Protein Group was listed in the column Residue sites. Accessions Names Sequences Scores sp P02787 TRFE_HUMAN sp P02787 TRFE_HUMAN sp P02787 TRFE_HUMAN sp P02787 TRFE_HUMAN sp P04114 APOB_HUMAN; tr A0A087WTM7 A0A087WTM7_HU MAN; sp P04114 APOB_HUMAN; tr A0A087WTM7 A0A087WTM7_HU MAN; Serotransferrin OS=Homo sapiens GN=TF PE=1 SV=3 Serotransferrin OS=Homo sapiens GN=TF PE=1 SV=3 Serotransferrin OS=Homo sapiens GN=TF PE=1 SV=3 Serotransferrin OS=Homo sapiens GN=TF PE=1 SV=3 Apolipoprotein B-100 OS=Homo sapiens GN=APOB PE=1 SV=2; Apolipoprotein B-100 OS=Homo sapiens GN=APOB PE=1 SV=1; Apolipoprotein B-100 OS=Homo sapiens GN=APOB PE=1 SV=2; Apolipoprotein B-100 OS=Homo sapiens GN=APOB PE=1 SV=1; Residue sites Uniprot notes QQQHLFGSNVTDCSGNFCLFR Yes CGLVPVLAENYNK Yes CGLVPVLAENYNK LCMGSGLNLCEPNNK FNSSYLQGTNQITGR Yes YDFNSSMLYSTAK Yes 25

26 sp P04114 APOB_HUMAN; tr A0A087WTM7 A0A087WTM7_HU MAN; sp P04114 APOB_HUMAN; tr A0A087WTM7 A0A087WTM7_HU MAN; sp P01024 CO3_HUMAN sp P01024 CO3_HUMAN sp P02751 FINC_HUMAN sp P02751 FINC_HUMAN sp P00738 HPT_HUMAN sp P00738 HPT_HUMAN sp P00738 HPT_HUMAN sp P00738 HPT_HUMAN Apolipoprotein B-100 OS=Homo sapiens GN=APOB PE=1 SV=2; Apolipoprotein B-100 OS=Homo sapiens GN=APOB PE=1 SV=1; Apolipoprotein B-100 OS=Homo sapiens GN=APOB PE=1 SV=2; Apolipoprotein B-100 OS=Homo sapiens GN=APOB PE=1 SV=1; Complement C3 OS=Homo sapiens GN=C3 PE=1 SV=2 Complement C3 OS=Homo sapiens GN=C3 PE=1 SV=2 Fibronectin OS=Homo sapiens GN=FN1 PE=1 SV=4 Fibronectin OS=Homo sapiens GN=FN1 PE=1 SV=4 Haptoglobin OS=Homo sapiens GN=HP Haptoglobin OS=Homo sapiens GN=HP Haptoglobin OS=Homo sapiens GN=HP Haptoglobin OS=Homo sapiens GN=HP FNSSYLQGTNQITGR FVEGSHNSTVSLTTK Yes TVLTPNHMGNVTFTIPANR Yes TVLTPNHMGNVTFTIPANR HEEGHMLNCTCFGQGR Yes RHEEGHMLNCTCFGQGR Yes NLFLNHSENAK Yes VVLHPNYSQVDIGLIK Yes NLFLNHSENAK Yes MVSHHNLTTGLINEQWLL TTAK Yes 26

27 sp P01857 IGHG1_HUMAN Ig gamma-1 chain C region OS=Homo sapiens GN=IGHG1 TKPREEQYNSTYR Yes sp P01857 IGHG1_HUMAN sp O75882 RN_HUMAN sp O75882 RN_HUMAN sp O75882 RN_HUMAN sp O75882 RN_HUMAN sp O75882 RN_HUMAN sp O75882 RN_HUMAN tr B4E1Z4 B4E1Z4_HUMAN tr B4E1Z4 B4E1Z4_HUMAN sp P00734 THRB_HUMAN; tr E9PIT3 E9PIT3_HUMAN; Ig gamma-1 chain C region OS=Homo sapiens GN=IGHG1 Attractin OS=Homo sapiens GN=RN PE=1 SV=2 Attractin OS=Homo sapiens GN=RN PE=1 SV=2 Attractin OS=Homo sapiens GN=RN PE=1 SV=2 Attractin OS=Homo sapiens GN=RN PE=1 SV=2 Attractin OS=Homo sapiens GN=RN PE=1 SV=2 Attractin OS=Homo sapiens GN=RN PE=1 SV=2 Uncharacterized protein OS=Homo sapiens Uncharacterized protein OS=Homo sapiens Prothrombin OS=Homo sapiens GN=F2 PE=1 SV=2; Prothrombin OS=Homo sapiens GN=F2 ; EEQYNSTYR Yes CINQSICEK Yes GICNSSDVR Yes NHSCSEGQISIFR Yes YNWSFIHCPACQCNGHSK Yes VFHIHNESWVLLTPK Yes IDSTGNVTNELR Yes QSVPAHFVALNGSK TMFPNLTDVR NFTENDLLVR Yes 27

28 sp P00734 THRB_HUMAN; tr E9PIT3 E9PIT3_HUMAN; Prothrombin OS=Homo sapiens GN=F2 PE=1 SV=2; Prothrombin OS=Homo sapiens GN=F2 ; YPHKPEINSTTHPGADLQENF CR Yes sp P00734 THRB_HUMAN; tr E9PIT3 E9PIT3_HUMAN; sp P08603 CFAH_HUMAN sp P08603 CFAH_HUMAN sp P08603 CFAH_HUMAN sp P08603 CFAH_HUMAN tr E9PFZ2 E9PFZ2_HUMAN; sp P00450 CERU_HUMAN Prothrombin OS=Homo sapiens GN=F2 PE=1 SV=2; Prothrombin OS=Homo sapiens GN=F2 ; Complement factor H OS=Homo sapiens GN=CFH PE=1 SV=4 Complement factor H OS=Homo sapiens GN=CFH PE=1 SV=4 Complement factor H OS=Homo sapiens GN=CFH PE=1 SV=4 Complement factor H OS=Homo sapiens GN=CFH PE=1 SV=4 Ceruloplasmin OS=Homo sapiens GN=CP ; Ceruloplasmin OS=Homo sapiens GN=CP GHVNITR Yes IPCSQPPQIEHGTINSSR Yes SPDVINGSPISQK Yes ISEENETTCYMGK Yes MDGASNVTCINSR Yes ELHHLQEQNVSNAFLDK Yes tr E9PFZ2 E9PFZ2_HUMAN; sp P00450 CERU_HUMAN Ceruloplasmin OS=Homo sapiens GN=CP ; Ceruloplasmin OS=Homo sapiens GN=CP EHEGAIYPDNTTDFQR Yes 28

29 sp P00450 CERU_HUMAN; tr E9PFZ2 E9PFZ2_HUMAN sp P01042 KNG1_HUMAN sp P01042 KNG1_HUMAN sp P01042 KNG1_HUMAN sp P01042 KNG1_HUMAN sp P01042 KNG1_HUMAN sp P01042 KNG1_HUMAN sp P01042 KNG1_HUMAN sp P01042 KNG1_HUMAN sp P07996 TSP1_HUMAN sp P07996 TSP1_HUMAN sp P07996 TSP1_HUMAN Ceruloplasmin OS=Homo sapiens GN=CP ; Ceruloplasmin OS=Homo sapiens GN=CP Kininogen-1 OS=Homo sapiens GN=KNG1 PE=1 SV=2 Kininogen-1 OS=Homo sapiens GN=KNG1 PE=1 SV=2 Kininogen-1 OS=Homo sapiens GN=KNG1 PE=1 SV=2 Kininogen-1 OS=Homo sapiens GN=KNG1 PE=1 SV=2 Kininogen-1 OS=Homo sapiens GN=KNG1 PE=1 SV=2 Kininogen-1 OS=Homo sapiens GN=KNG1 PE=1 SV=2 Kininogen-1 OS=Homo sapiens GN=KNG1 PE=1 SV=2 Kininogen-1 OS=Homo sapiens GN=KNG1 PE=1 SV=2 Thrombospondin-1 OS=Homo sapiens GN=THBS1 PE=1 SV=2 Thrombospondin-1 OS=Homo sapiens GN=THBS1 PE=1 SV=2 Thrombospondin-1 OS=Homo sapiens GN=THBS1 PE=1 SV=2 ENLTAPGSDSAVFFEQGTTR Yes ITYSIVQTNCSK Yes KYNSQNQSNNQFVLYR Yes KYNSQNQSNNQFVLYR 7 45 YNSQNQSNNQFVLYR YNSQNQSNNQFVLYR YNSQNQSNNQFVLYR Yes LNAENNFYFK Yes LNAENNFYFK KVSCPIMPCSNVPDGECCP R Yes VSCPIMPCSNVPDGECCPR Yes VVNSTTGPGEHLR Yes 29

30 tr F5GXS0 F5GXS0_HUMAN; tr A0A140TA49 A0A140TA49_HUM AN; tr A0A140TA44 A0A140TA44_HUM AN; tr A0A140TA32 A0A140TA32_HUM AN; tr A0A140TA29 A0A140TA29_HUM AN; tr A0A0G2JPR0 A0A0G2JPR0_HUM AN; tr A0A0G2JL54 A0A0G2JL54_HUM AN; sp P0C0L5 CO4B_HUMAN; sp P0C0L4 CO4A_HUMAN Complement C4-B OS=Homo sapiens GN=C4B ; Complement C4-A OS=Homo sapiens GN=C4A ; Complement C4-A OS=Homo sapiens GN=C4A ; Complement C4-A OS=Homo sapiens GN=C4A ; Complement C4-B OS=Homo sapiens GN=C4B ; Complement C4-A OS=Homo sapiens GN=C4A ; Complement C4-B OS=Homo sapiens GN=C4B_2 ; Complement C4-B OS=Homo sapiens GN=C4B PE=1 SV=2; Complement C4-A OS=Homo sapiens GN=C4A PE=1 SV=2 FSDGLESNSSTQFEVK Yes 30

31 sp P0C0L5 CO4B_HUMAN; sp P0C0L4 CO4A_HUMAN; tr F5GXS0 F5GXS0_HUMAN; tr A0A140TA49 A0A140TA49_HUM AN; tr A0A140TA44 A0A140TA44_HUM AN; tr A0A140TA32 A0A140TA32_HUM AN; tr A0A140TA29 A0A140TA29_HUM AN; tr A0A0G2JPR0 A0A0G2JPR0_HUM AN; tr A0A0G2JL54 A0A0G2JL54_HUM AN; Complement C4-B OS=Homo sapiens GN=C4B PE=1 SV=2; Complement C4-A OS=Homo sapiens GN=C4A PE=1 SV=2; Complement C4-B OS=Homo sapiens GN=C4B ; Complement C4-A OS=Homo sapiens GN=C4A ; Complement C4-A OS=Homo sapiens GN=C4A ; Complement C4-A OS=Homo sapiens GN=C4A ; Complement C4-B OS=Homo sapiens GN=C4B ; Complement C4-A OS=Homo sapiens GN=C4A ; Complement C4-B OS=Homo sapiens GN=C4B_2 ; GLNVTLSSTGR Yes tr H0YAC1 H0YAC1_HUMAN tr H0YAC1 H0YAC1_HUMAN tr H0YAC1 H0YAC1_HUMAN Plasma kallikrein (Fragment) OS=Homo sapiens GN=KLKB1 Plasma kallikrein (Fragment) OS=Homo sapiens GN=KLKB1 Plasma kallikrein (Fragment) OS=Homo sapiens GN=KLKB1 IYSGILNLSDITK GVNFNVSK LQAPLNYTEFQKPICLPSK

32 tr H0YAC1 H0YAC1_HUMAN sp P43652 AFAM_HUMAN sp P43652 AFAM_HUMAN sp P43652 AFAM_HUMAN sp P05156 CFAI_HUMAN; tr G3XAM2 G3XAM2_HUMAN; tr E7ETH0 E7ETH0_HUMAN; Plasma kallikrein (Fragment) OS=Homo sapiens GN=KLKB1 Afamin OS=Homo sapiens GN=AFM PE=1 SV=1 Afamin OS=Homo sapiens GN=AFM PE=1 SV=1 Afamin OS=Homo sapiens GN=AFM PE=1 SV=1 Complement factor I OS=Homo sapiens GN=CFI PE=1 SV=2; Complement factor I OS=Homo sapiens GN=CFI ; Complement factor I OS=Homo sapiens GN=CFI ; GVNVCQETCTK DIENFNSTQK Yes YAEDKFNETTEK Yes NCCNTENPPGCYR Yes FLNNGTCTAEGK Yes sp P05156 CFAI_HUMAN; tr G3XAM2 G3XAM2_HUMAN; tr E7ETH0 E7ETH0_HUMAN Complement factor I OS=Homo sapiens GN=CFI PE=1 SV=2; Complement factor I OS=Homo sapiens GN=CFI ; Complement factor I OS=Homo sapiens GN=CFI ; NGTAVCNRR Yes sp P01877 IGHA2_HUMAN; tr A0A0G2JMB2 A0A0G2JMB2_HU MAN; Ig alpha-2 chain C region OS=Homo sapiens GN=IGHA2 PE=1 SV=3; Ig alpha-2 chain C region (Fragment) OS=Homo sapiens GN=IGHA2 ; TPLTANITK Yes 32

33 sp Q13201 MMRN1_HUMAN sp Q13201 MMRN1_HUMAN sp Q13201 MMRN1_HUMAN sp Q08380 LG3BP_HUMAN Multimerin-1 OS=Homo sapiens GN=MMRN1 PE=1 SV=3 Multimerin-1 OS=Homo sapiens GN=MMRN1 PE=1 SV=3 Multimerin-1 OS=Homo sapiens GN=MMRN1 PE=1 SV=3 Galectin-3-binding protein OS=Homo sapiens GN=LGALS3BP FNPGAESVVLSNSTLK Yes HPFTGDNCTIK Yes MYQMFNETTSQVR Yes AAIPSALDTNSSK Yes sp Q08380 LG3BP_HUMAN Galectin-3-binding protein OS=Homo sapiens GN=LGALS3BP TVIRPFYLTNSSGVD Yes sp Q08380 LG3BP_HUMAN Galectin-3-binding protein OS=Homo sapiens GN=LGALS3BP ALGFENQALGR Yes sp Q08380 LG3BP_HUMAN sp P02749 APOH_HUMAN sp P02749 APOH_HUMAN sp P02749 APOH_HUMAN Galectin-3-binding protein OS=Homo sapiens GN=LGALS3BP Beta-2-glycoprotein 1 OS=Homo sapiens GN=APOH PE=1 SV=3 Beta-2-glycoprotein 1 OS=Homo sapiens GN=APOH PE=1 SV=3 Beta-2-glycoprotein 1 OS=Homo sapiens GN=APOH PE=1 SV=3 GLNLTEDTYKPR Yes LGNWSAMPSCK Yes VYKPSAGNNSLYR Yes VYKPSAGNNSLYR

34 sp P04196 HRG_HUMAN Histidine-rich glycoprotein OS=Homo sapiens GN=HRG HSHNNNSSDLHPHK sp P04196 HRG_HUMAN Histidine-rich glycoprotein OS=Homo sapiens GN=HRG HSHNNNSSDLHPHK sp P04196 HRG_HUMAN Histidine-rich glycoprotein OS=Homo sapiens GN=HRG HSHNNNSSDLHPHK sp P04196 HRG_HUMAN sp P10909 CLUS_HUMAN sp P10909 CLUS_HUMAN sp P10909 CLUS_HUMAN sp P01859 IGHG2_HUMAN; tr A0A0G2JN06 A0A0G2JN06_HUM AN; Histidine-rich glycoprotein OS=Homo sapiens GN=HRG Clusterin OS=Homo sapiens GN=CLU PE=1 SV=1 Clusterin OS=Homo sapiens GN=CLU PE=1 SV=1 Clusterin OS=Homo sapiens GN=CLU PE=1 SV=1 Ig gamma-2 chain C region OS=Homo sapiens GN=IGHG2 PE=1 SV=2; Ig gamma-2 chain C region (Fragment) OS=Homo sapiens GN=IGHG2 PE=1 SV=2; VIDFNCTTSSVSSALANTK Yes HNSTGCLR Yes LANLTQGEDQYYLR Yes KKEDALNETR 5 86 Yes EEQFNSTFR Yes 34

35 sp P01859 IGHG2_HUMAN; tr A0A0G2JN06 A0A0G2JN06_HUM AN; sp P03951 FA11_HUMAN sp P03951 FA11_HUMAN sp Q9Y6R7 FCGBP_HUMAN; tr A0A087WXI2 A0A087WXI2_HUM AN; sp P02790 HEMO_HUMAN sp P02790 HEMO_HUMAN Ig gamma-2 chain C region OS=Homo sapiens GN=IGHG2 PE=1 SV=2; Ig gamma-2 chain C region (Fragment) OS=Homo sapiens GN=IGHG2 PE=1 SV=2; Coagulation factor XI OS=Homo sapiens GN=F11 Coagulation factor XI OS=Homo sapiens GN=F11 IgGFc-binding protein OS=Homo sapiens GN=FCGBP PE=1 SV=3; IgGFc-binding protein OS=Homo sapiens GN=FCGBP ; Hemopexin OS=Homo sapiens GN=HPX PE=1 SV=2 Hemopexin OS=Homo sapiens GN=HPX PE=1 SV=2 TKPREEQFNSTFR Yes GINYNSSVAK Yes VYSGILNQSEIK Yes YLPVNSSLLTSDCSER SWPAVGNCSSALR Yes ALPQPQNVTSLLGCTH Yes sp P05090 APOD_HUMAN; tr C9JX71 C9JX71_HUMAN; tr C9JF17 C9JF17_HUMAN; Apolipoprotein D OS=Homo sapiens GN=APOD ; Apolipoprotein D (Fragment) OS=Homo sapiens GN=APOD ; Apolipoprotein D (Fragment) OS=Homo sapiens GN=APOD ; CIQANYSLMENGK Yes 35

36 sp P05090 APOD_HUMAN; tr C9JX71 C9JX71_HUMAN; tr C9JF17 C9JF17_HUMAN; Apolipoprotein D OS=Homo sapiens GN=APOD ; Apolipoprotein D (Fragment) OS=Homo sapiens GN=APOD ; Apolipoprotein D (Fragment) OS=Homo sapiens GN=APOD ; CIQANYSLMENGK sp P05090 APOD_HUMAN; tr C9JX71 C9JX71_HUMAN; tr C9JF17 C9JF17_HUMAN; Apolipoprotein D OS=Homo sapiens GN=APOD ; Apolipoprotein D (Fragment) OS=Homo sapiens GN=APOD ; Apolipoprotein D (Fragment) OS=Homo sapiens GN=APOD ; ADGTVNQIEGEPVNLTEPA K Yes sp P02765 FETUA_HUMAN; tr C9JV77 C9JV77_HUMAN; sp P02765 FETUA_HUMAN; tr C9JV77 C9JV77_HUMAN; Alpha-2-HS-glycoprotein OS=Homo sapiens GN=AHSG ; Alpha-2-HS-glycoprotein OS=Homo sapiens GN=AHSG ; Alpha-2-HS-glycoprotein OS=Homo sapiens GN=AHSG ; Alpha-2-HS-glycoprotein OS=Homo sapiens GN=AHSG ; KVCQDCPLLAPLNDTR Yes VCQDCPLLAPLNDTR Yes 36

37 sp P23142 FBLN1_HUMAN; tr B1AHL2 B1AHL2_HUMAN; sp P49908 SEPP1_HUMAN sp P49908 SEPP1_HUMAN sp P49908 SEPP1_HUMAN sp P01008 ANT3_HUMAN sp P04275 VWF_HUMAN sp P04275 VWF_HUMAN sp P04004 VTNC_HUMAN sp P04004 VTNC_HUMAN sp P04004 VTNC_HUMAN sp P01833 PIGR_HUMAN Fibulin-1 OS=Homo sapiens GN=FBLN1 PE=1 SV=4; Fibulin-1 OS=Homo sapiens GN=FBLN1 ; Selenoprotein P OS=Homo sapiens GN=SEPP1 PE=1 SV=3 Selenoprotein P OS=Homo sapiens GN=SEPP1 PE=1 SV=3 Selenoprotein P OS=Homo sapiens GN=SEPP1 PE=1 SV=3 Antithrombin-III OS=Homo sapiens GN=SERPINC1 von Willebrand factor OS=Homo sapiens GN=VWF PE=1 SV=4 von Willebrand factor OS=Homo sapiens GN=VWF PE=1 SV=4 Vitronectin OS=Homo sapiens GN=VTN Vitronectin OS=Homo sapiens GN=VTN Vitronectin OS=Homo sapiens GN=VTN Polymeric immunoglobulin receptor OS=Homo sapiens GN=PIGR PE=1 SV=4 CPHGDNASLEFVK Yes CGNCSLTTLK Yes CGNCSLTTLKDEDFCK Yes CGNCSLTTLKDEDFCKR Yes WVSNKTEGR Yes HCDGNVSSCGDHPSEGCFCPP DK Yes MEACMLNGTVIGPGK Yes NGSLFAFR Yes NNVHEQVGGPSLTSDLQA QSK NNVHEQVGGPSLTSDLQA QSK Yes VPGNVTAVLGETLK Yes 37

38 sp P01833 PIGR_HUMAN Polymeric immunoglobulin receptor OS=Homo sapiens GN=PIGR PE=1 SV=4 WNNTGCQALPSQDEGPSK Yes sp P01833 PIGR_HUMAN Polymeric immunoglobulin receptor OS=Homo sapiens GN=PIGR PE=1 SV=4 WNNTGCQALPSQDEGPSK sp P55058 PLTP_HUMAN Phospholipid transfer protein OS=Homo sapiens GN=PLTP VSNVSCQASVSR Yes sp P55058 PLTP_HUMAN Phospholipid transfer protein OS=Homo sapiens GN=PLTP NWSLPNR sp P55058 PLTP_HUMAN sp P00748 FA12_HUMAN sp P00748 FA12_HUMAN sp P01009 A1_HUMAN Phospholipid transfer protein OS=Homo sapiens GN=PLTP Coagulation factor XII OS=Homo sapiens GN=F12 PE=1 SV=3 Coagulation factor XII OS=Homo sapiens GN=F12 PE=1 SV=3 Alpha-1-antitrypsin OS=Homo sapiens GN=SERPINA1 PE=1 SV=3 EGHFYYNISEVK Yes NHSCEPCQTLAVR Yes RNHSCEPCQTLAVR Yes YLGNAIFFLPDEGK Yes sp P05160 F13B_HUMAN Coagulation factor XIII B chain OS=Homo sapiens GN=F13B PE=1 SV=3 KEHETCLAPELYNGNYSTTQ K Yes 38

39 sp P01860 IGHG3_HUMAN; tr A0A087WXL8 A0A087WXL8_HU MAN; tr A0A087WVW2 A0A087WVW2_H UMAN; tr A0A075B6N8 A0A075B6N8_HUM AN; Ig gamma-3 chain C region OS=Homo sapiens GN=IGHG3 PE=1 SV=2; Ig gamma-3 chain C region OS=Homo sapiens GN=IGHG3 ; Ig gamma-3 chain C region OS=Homo sapiens GN=IGHG3 ; Ig gamma-3 chain C region (Fragment) OS=Homo sapiens GN=IGHG3 ; EEQYNSTFR Yes sp Q9UGM5 FETUB_HUMAN sp P80108 PHLD_HUMAN sp P80108 PHLD_HUMAN sp P22792 CPN2_HUMAN Fetuin-B OS=Homo sapiens GN=FETUB PE=1 SV=2 Phosphatidylinositol-glycan-specific phospholipase D OS=Homo sapiens GN=GPLD1 PE=1 SV=3 Phosphatidylinositol-glycan-specific phospholipase D OS=Homo sapiens GN=GPLD1 PE=1 SV=3 Carboxypeptidase N subunit 2 OS=Homo sapiens GN=CPN2 PE=1 SV=3 VLYLAAYNCTLRPVSK Yes LNVEAANWTVR Yes NLTTSLTESVDR Yes AFGSNPNLTK Yes sp P05546 HEP2_HUMAN Heparin cofactor 2 OS=Homo sapiens GN=SERPIND1 PE=1 SV=3 NLSMPLLPADFHK 9 49 Yes 39

40 sp P25311 ZA2G_HUMAN; tr C9JEV0 C9JEV0_HUMAN; Zinc-alpha-2-glycoprotein OS=Homo sapiens GN=AZGP1 PE=1 SV=2; Zinc-alpha-2-glycoprotein OS=Homo sapiens GN=AZGP1 ; DIVEYYNDSNGSHVLQGR Yes sp P25311 ZA2G_HUMAN; tr C9JEV0 C9JEV0_HUMAN; Zinc-alpha-2-glycoprotein OS=Homo sapiens GN=AZGP1 PE=1 SV=2; Zinc-alpha-2-glycoprotein OS=Homo sapiens GN=AZGP1 ; DIVEYYNDSNGSHVLQGR Yes sp P25311 ZA2G_HUMAN; tr C9JEV0 C9JEV0_HUMAN; sp P02760 AMBP_HUMAN sp P02760 AMBP_HUMAN sp P02750 A2GL_HUMAN Zinc-alpha-2-glycoprotein OS=Homo sapiens GN=AZGP1 PE=1 SV=2; Zinc-alpha-2-glycoprotein OS=Homo sapiens GN=AZGP1 ; Protein AMBP OS=Homo sapiens GN=AMBP Protein AMBP OS=Homo sapiens GN=AMBP Leucine-rich alpha-2-glycoprotein OS=Homo sapiens GN=LRG1 PE=1 SV=2 FGCEIENNR Yes CVLFPYGGCQGNGNK CVLFPYGGCQGNGNK KLPPGLLANFTLLR Yes sp P02750 A2GL_HUMAN Leucine-rich alpha-2-glycoprotein OS=Homo sapiens GN=LRG1 PE=1 SV=2 MFSQNDTR Yes 40

41 sp P20851 C4BPB_HUMAN; tr Q5VVQ7 Q5VVQ7_HUMAN; sp P20851 C4BPB_HUMAN; tr Q5VVQ7 Q5VVQ7_HUMAN; sp Q04756 HGFA_HUMAN; tr D6RAR4 D6RAR4_HUMAN; C4b-binding protein beta chain OS=Homo sapiens GN=C4BPB ; C4b-binding protein beta chain (Fragment) OS=Homo sapiens GN=C4BPB C4b-binding protein beta chain OS=Homo sapiens GN=C4BPB ; C4b-binding protein beta chain (Fragment) OS=Homo sapiens GN=C4BPB Hepatocyte growth factor activator OS=Homo sapiens GN=HGFAC PE=1 SV=1; Hepatocyte growth factor activator OS=Homo sapiens GN=HGFAC KTLFCNASK 9 64 Yes TLFCNASK 8 64 Yes CFLGNGTGYR Yes sp P01011 AACT_HUMAN; tr G3V3A0 G3V3A0_HUMAN; Alpha-1-antichymotrypsin OS=Homo sapiens GN=SERPINA3 PE=1 SV=2; Alpha-1-antichymotrypsin OS=Homo sapiens GN=SERPINA3 YTGNASALFILPDQDK Yes sp Q6UY14 L4_HUMAN ADAMTS-like protein 4 OS=Homo sapiens GN=ADAMTSL4 PE=1 SV=2 LVSGNLTDR Yes 41

42 sp Q9UK55 ZPI_HUMAN; tr G3V2W1 G3V2W1_HUMAN; Protein Z-dependent protease inhibitor OS=Homo sapiens GN=SERPINA10 PE=1 SV=1; Protein Z-dependent protease inhibitor OS=Homo sapiens GN=SERPINA10 PE=1 SV=1; ETFFNLSKR Yes sp Q9UK55 ZPI_HUMAN; tr G3V2W1 G3V2W1_HUMAN; Protein Z-dependent protease inhibitor OS=Homo sapiens GN=SERPINA10 PE=1 SV=1; Protein Z-dependent protease inhibitor OS=Homo sapiens GN=SERPINA10 PE=1 SV=1; ETFFNLSK Yes sp Q9Y5Y7 LYVE1_HUMAN Lymphatic vessel endothelial hyaluronic acid receptor 1 OS=Homo sapiens GN=LYVE1 PE=1 SV=2 KANQQLNFTEAK Yes sp Q9Y5Y7 LYVE1_HUMAN sp P43251 BTD_HUMAN sp P43251 BTD_HUMAN Lymphatic vessel endothelial hyaluronic acid receptor 1 OS=Homo sapiens GN=LYVE1 PE=1 SV=2 Biotinidase OS=Homo sapiens GN=BTD PE=1 SV=2 Biotinidase OS=Homo sapiens GN=BTD PE=1 SV=2 ANQQLNFTEAK 7 53 Yes FNDTEVLQR Yes NPVGLIGAENGETDPSHSK Yes 42

43 sp P01591 IGJ_HUMAN; tr D6RHJ6 D6RHJ6_HUMAN; tr D6RD17 D6RD17_HUMAN; sp P01591 IGJ_HUMAN; tr D6RHJ6 D6RHJ6_HUMAN; tr D6RD17 D6RD17_HUMAN; sp P01591 IGJ_HUMAN; tr D6RHJ6 D6RHJ6_HUMAN; tr D6RD17 D6RD17_HUMAN; Immunoglobulin J chain OS=Homo sapiens GN=JCHAIN PE=1 SV=4; Immunoglobulin J chain (Fragment) OS=Homo sapiens GN=JCHAIN ; Immunoglobulin J chain (Fragment) OS=Homo sapiens GN=JCHAIN PE=1 SV=7; Immunoglobulin J chain OS=Homo sapiens GN=JCHAIN PE=1 SV=4; Immunoglobulin J chain (Fragment) OS=Homo sapiens GN=JCHAIN ; Immunoglobulin J chain (Fragment) OS=Homo sapiens GN=JCHAIN PE=1 SV=7; Immunoglobulin J chain OS=Homo sapiens GN=JCHAIN PE=1 SV=4; Immunoglobulin J chain (Fragment) OS=Homo sapiens GN=JCHAIN ; Immunoglobulin J chain (Fragment) OS=Homo sapiens GN=JCHAIN PE=1 SV=7; ENISDPTSPLR Yes IIVPLNNRENISDPTSPLR Yes IIVPLNNRENISDPTSPLR

44 sp P01591 IGJ_HUMAN; tr D6RHJ6 D6RHJ6_HUMAN; tr D6RD17 D6RD17_HUMAN; Immunoglobulin J chain OS=Homo sapiens GN=JCHAIN PE=1 SV=4; Immunoglobulin J chain (Fragment) OS=Homo sapiens GN=JCHAIN ; Immunoglobulin J chain (Fragment) OS=Homo sapiens GN=JCHAIN PE=1 SV=7; IIVPLNNRENISDPTSPLR 7 68 tr I3L4B9 I3L4B9_HUMAN; tr I3L2X4 I3L2X4_HUMAN; tr I3L1J1 I3L1J1_HUMAN; tr I3L145 I3L145_HUMAN; tr B0FWH6 B0FWH6_HUMAN; tr A0A0C4DGN2 A0A0C4DGN2_HU MAN; sp P04278 SHBG_HUMAN Sex hormone-binding globulin OS=Homo sapiens GN=SHBG ; Sex hormone-binding globulin OS=Homo sapiens GN=SHBG ; Sex hormone-binding globulin OS=Homo sapiens GN=SHBG ; Sex hormone-binding globulin OS=Homo sapiens GN=SHBG ; Sex hormone binding globulin (Fragment) OS=Homo sapiens GN=SHBG ; Sex hormone-binding globulin (Fragment) OS=Homo sapiens GN=SHBG ; Sex hormone-binding globulin OS=Homo sapiens GN=SHBG PE=1 SV=2 SHEIWTHSCPQSPGNGTDASH Yes 44

45 sp P06276 CHLE_HUMAN; tr F8WF14 F8WF14_HUMAN; tr F8WEX7 F8WEX7_HUMAN; Cholinesterase OS=Homo sapiens GN=BCHE ; Carboxylic ester hydrolase OS=Homo sapiens GN=BCHE ; Carboxylic ester hydrolase OS=Homo sapiens GN=BCHE ; DNNSIITR Yes sp P06276 CHLE_HUMAN; tr F8WF14 F8WF14_HUMAN; tr F8WEX7 F8WEX7_HUMAN; Cholinesterase OS=Homo sapiens GN=BCHE ; Carboxylic ester hydrolase OS=Homo sapiens GN=BCHE ; Carboxylic ester hydrolase OS=Homo sapiens GN=BCHE ; WSDIWNK Yes sp Q92954 PRG4_HUMAN; tr A0A0U1RR20 A0A0U1RR20_HU MAN; sp P13671 CO6_HUMAN sp P01861 IGHG4_HUMAN; tr A0A0G2JPD4 A0A0G2JPD4_HUM AN; Proteoglycan 4 OS=Homo sapiens GN=PRG4 PE=1 SV=2; Proteoglycan 4 OS=Homo sapiens GN=PRG4 ; Complement component C6 OS=Homo sapiens GN=C6 PE=1 SV=3 Ig gamma-4 chain C region OS=Homo sapiens GN=IGHG4 ; Ig gamma-4 chain C region (Fragment) OS=Homo sapiens GN=IGHG4 PE=1 SV=2; NGTLVAFR Yes VLNFTTK Yes EEQFNSTYR Yes 45

46 sp P01877 IGHA2_HUMAN; tr A0A075B6N7 A0A075B6N7_HUM AN Ig alpha-2 chain C region (Fragment) OS=Homo sapiens GN=IGHA2 ; Ig alpha-2 chain C region (Fragment) OS=Homo sapiens GN=IGHA2 HYTNSSQDVTVPCR Yes sp Q12884 SEPR_HUMAN; tr A0A0D9SEN1 A0A0D9SEN1_HU MAN; sp P16070 CD44_HUMAN sp P49747 COMP_HUMAN; tr G3XAP6 G3XAP6_HUMAN; Prolyl endopeptidase FAP OS=Homo sapiens GN=FAP PE=1 SV=5; Prolyl endopeptidase FAP OS=Homo sapiens GN=FAP PE=1 SV=1; CD44 antigen OS=Homo sapiens GN=CD44 PE=1 SV=3 Cartilage oligomeric matrix protein OS=Homo sapiens GN=COMP PE=1 SV=2; Cartilage oligomeric matrix protein OS=Homo sapiens GN=COMP ; SVNASNYGLSPDR Yes AFNSTLPTMAQMEK Yes CNDTIPEDYETHQLR Yes sp Q06033 ITIH3_HUMAN; tr A0A087WW43 A0A087WW43_HU MAN Inter-alpha-trypsin inhibitor heavy chain H3 OS=Homo sapiens GN=ITIH3 ; Inter-alpha-trypsin inhibitor heavy chain H3 OS=Homo sapiens GN=ITIH3 NAHGEEKENLTAR Yes sp Q7Z7M0 MEGF8_HUMAN Multiple epidermal growth factor-like domains protein 8 OS=Homo sapiens GN=MEGF8 PE=1 SV=2 GYQGDGISHCNR

47 sp Q76LX8 S13_HUMAN sp P08519 APOA_HUMAN A disintegrin and metalloproteinase with thrombospondin motifs 13 OS=Homo sapiens GN=ADAMTS13 Apolipoprotein(a) OS=Homo sapiens GN=LPA MSISPNTTYPSLLEDGR Yes WEYCNLTR Yes sp P05155 IC1_HUMAN; tr H9KV48 H9KV48_HUMAN; tr E9PGN7 E9PGN7_HUMAN; Plasma protease C1 inhibitor OS=Homo sapiens GN=SERPING1 PE=1 SV=2; Plasma protease C1 inhibitor OS=Homo sapiens GN=SERPING1 ; Plasma protease C1 inhibitor OS=Homo sapiens GN=SERPING1 ; DTFVNASR Yes sp P26927 HGFL_HUMAN; tr G3XAK1 G3XAK1_HUMAN; tr E7EX60 E7EX60_HUMAN Hepatocyte growth factor-like protein OS=Homo sapiens GN=MST1 PE=1 SV=2; Hepatocyte growth factor-like protein OS=Homo sapiens GN=MST1 ; Neuropilin-1 OS=Homo sapiens GN=NRP1 GTANTTTAGVPCQR Yes RGPECSQNYTTPSGVIK

48 sp P17936 IBP3_HUMAN; tr A6XND1 A6XND1_HUMAN; tr A6XND0 A6XND0_HUMAN; sp Q6YHK3 CD109_HUMAN sp Q16610 ECM1_HUMAN Insulin-like growth factor-binding protein 3 OS=Homo sapiens GN=IGFBP3 PE=1 SV=2; Insulin-like growth factor binding protein 3 isoform b OS=Homo sapiens GN=IGFBP3 ; Insulin-like growth factor binding protein 3 OS=Homo sapiens GN=IGFBP3 ; CD109 antigen OS=Homo sapiens GN=CD109 PE=1 SV=2 Extracellular matrix protein 1 OS=Homo sapiens GN=ECM1 PE=1 SV=2 GLCVNASAVSR Yes HLNGTITAK Yes HIPGLIHNMTAR Yes sp P36980 FHR2_HUMAN; tr V9GYE7 V9GYE7_HUMAN; Complement factor H-related protein 2 OS=Homo sapiens GN=CFHR2 ; Complement factor H-related protein 2 OS=Homo sapiens GN=CFHR2 ; LQNNENNISCVER Yes sp P36980 FHR2_HUMAN; tr V9GYE7 V9GYE7_HUMAN; Complement factor H-related protein 2 OS=Homo sapiens GN=CFHR2 ; Complement factor H-related protein 2 OS=Homo sapiens GN=CFHR2 ; LQNNENNISCVER

49 tr H7BYX9 H7BYX9_HUMAN; tr E7END6 E7END6_HUMAN; sp P04070 PROC_HUMAN Vitamin K-dependent protein C (Fragment) OS=Homo sapiens GN=PROC ; Vitamin K-dependent protein C OS=Homo sapiens GN=PROC ; Vitamin K-dependent protein C OS=Homo sapiens GN=PROC EVFVHPNYSK Yes tr H0Y512 H0Y512_HUMAN; sp Q9HDC9 APMAP_HUMAN Adipocyte plasma membrane-associated protein (Fragment) OS=Homo sapiens GN=APMAP ; Adipocyte plasma membrane-associated protein OS=Homo sapiens GN=APMAP PE=1 SV=2 AGPNGTLFVADAYK Yes sp P07358 CO8B_HUMAN; tr F5H7G1 F5H7G1_HUMAN; tr F5GY80 F5GY80_HUMAN; Complement component C8 beta chain OS=Homo sapiens GN=C8B PE=1 SV=3; Complement component C8 beta chain OS=Homo sapiens GN=C8B ; Complement component C8 beta chain OS=Homo sapiens GN=C8B ; LLCNGDNDCGDQSDEANCR

50 sp P07358 CO8B_HUMAN; tr F5H7G1 F5H7G1_HUMAN; tr F5GY80 F5GY80_HUMAN; Complement component C8 beta chain OS=Homo sapiens GN=C8B PE=1 SV=3; Complement component C8 beta chain OS=Homo sapiens GN=C8B ; Complement component C8 beta chain OS=Homo sapiens GN=C8B ; LLCNGDNDCGDQSDEANCR sp P51884 LUM_HUMAN sp P51884 LUM_HUMAN sp P51884 LUM_HUMAN sp P27918 PROP_HUMAN sp P27487 DPP4_HUMAN sp P01019 ANGT_HUMAN Lumican OS=Homo sapiens GN=LUM PE=1 SV=2 Lumican OS=Homo sapiens GN=LUM PE=1 SV=2 Lumican OS=Homo sapiens GN=LUM PE=1 SV=2 Properdin OS=Homo sapiens GN=CFP PE=1 SV=2 Dipeptidyl peptidase 4 OS=Homo sapiens GN=DPP4 PE=1 SV=2 Angiotensinogen OS=Homo sapiens GN=AGT LHINHNNLTESVGPLPK LHINHNNLTESVGPLPK LHINHNNLTESVGPLPK Yes NVTFWGRPLPR Yes KLDFIILNETK Yes LQAILGVPWKDKNCTSR Yes sp P80188 NGAL_HUMAN; tr X6R8F3 X6R8F3_HUMAN; Neutrophil gelatinase-associated lipocalin OS=Homo sapiens GN=LCN2 PE=1 SV=2; Neutrophil gelatinase-associated lipocalin OS=Homo sapiens GN=LCN2 ; SYNVTSVLFR Yes 50

51 sp Q15063 POSTN_HUMAN; tr B1ALD9 B1ALD9_HUMAN; sp Q9UNW1 MINP1_HUMAN sp Q9UHG3 PCYOX_HUMAN sp Q6EMK4 VASN_HUMAN sp P43121 MUC18_HUMAN sp P29622 KAIN_HUMAN sp P27169 PON1_HUMAN sp P19652 A1AG2_HUMAN sp P19652 A1AG2_HUMAN Periostin OS=Homo sapiens GN=POSTN PE=1 SV=2; Periostin OS=Homo sapiens GN=POSTN ; Multiple inositol polyphosphate phosphatase 1 OS=Homo sapiens GN=MINPP1 PE=1 SV=1 Prenylcysteine oxidase 1 OS=Homo sapiens GN=PCYOX1 PE=1 SV=3 Vasorin OS=Homo sapiens GN=VASN Cell surface glycoprotein MUC18 OS=Homo sapiens GN=MCAM PE=1 SV=2 Kallistatin OS=Homo sapiens GN=SERPINA4 PE=1 SV=3 Serum paraoxonase/arylesterase 1 OS=Homo sapiens GN=PON1 PE=1 SV=3 Alpha-1-acid glycoprotein 2 OS=Homo sapiens GN=ORM2 PE=1 SV=2 Alpha-1-acid glycoprotein 2 OS=Homo sapiens GN=ORM2 PE=1 SV=2 EVNDTLLVNELK Yes NALYHVEAFK Yes GELNTSIFSSRPIDK Yes LHEITNETFR Yes CVASVPSIPGLNR Yes FLNDTMAVYEAK Yes HANWTLTPLK Yes QNQCFYNSSYLNVQR Yes QNQCFYNSSYLNVQR

52 sp P02763 A1AG1_HUMAN sp P12259 FA5_HUMAN; tr A0A0A0MRJ7 A0A0A0MRJ7_HU MAN; sp P05543 THBG_HUMAN sp P28906 CD34_HUMAN Alpha-1-acid glycoprotein 1 OS=Homo sapiens GN=ORM1 Coagulation factor V OS=Homo sapiens GN=F5 PE=1 SV=4; Coagulation factor V OS=Homo sapiens GN=F5 ; Thyroxine-binding globulin OS=Homo sapiens GN=SERPINA7 PE=1 SV=2 Hematopoietic progenitor cell antigen CD34 OS=Homo sapiens GN=CD34 PE=1 SV=2 QDQCIYNTTYLNVQR Yes TWNQSIALR Yes VTACHSSQPNLYK Yes LTQGICLEQNK Yes sp Q13822 ENPP2_HUMAN; tr E7EUF1 E7EUF1_HUMAN; tr E5RIA2 E5RIA2_HUMAN; sp P15144 AMPN_HUMAN Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 OS=Homo sapiens GN=ENPP2 PE=1 SV=3; Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 OS=Homo sapiens GN=ENPP2 ; Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 OS=Homo sapiens GN=ENPP2 ; Aminopeptidase N OS=Homo sapiens GN=ANPEP PE=1 SV=4 AIIANLTCK Yes NLVNEADKLR Yes 52

53 sp P15144 AMPN_HUMAN sp P00736 C1R_HUMAN; tr F5H2D0 F5H2D0_HUMAN; tr B4DPQ0 B4DPQ0_HUMAN; Aminopeptidase N OS=Homo sapiens GN=ANPEP PE=1 SV=4 Complement C1r subcomponent OS=Homo sapiens GN=C1R PE=1 SV=2; Complement C1r subcomponent OS=Homo sapiens GN=C1R PE=1 SV=3; Complement C1r subcomponent OS=Homo sapiens GN=C1R ; KLNYTLSQGHR Yes CNYSIR Yes sp P08571 CD14_HUMAN; tr D6RFL4 D6RFL4_HUMAN; sp P16109 LYAM3_HUMAN; tr Q5R349 Q5R349_HUMAN; tr Q5R345 Q5R345_HUMAN; tr Q5R341 Q5R341_HUMAN; tr F6VVT6 F6VVT6_HUMAN; sp O00391 QSOX1_HUMAN Monocyte differentiation antigen CD14 OS=Homo sapiens GN=CD14 PE=1 SV=2; Monocyte differentiation antigen CD14 (Fragment) OS=Homo sapiens GN=CD14 ; P-selectin OS=Homo sapiens GN=SELP PE=1 SV=3; P-selectin (Fragment) OS=Homo sapiens GN=SELP PE=1 SV=2; P-selectin OS=Homo sapiens GN=SELP ; P-selectin OS=Homo sapiens GN=SELP ; P-selectin (Fragment) OS=Homo sapiens GN=SELP ; Sulfhydryl oxidase 1 OS=Homo sapiens GN=QSOX1 PE=1 SV=3 NVSWGR Yes AYSWNISR 6 54 Yes NSTLDPGKPEMMK Yes 53

54 sp P10643 CO7_HUMAN tr A0A087X0M8 A0A087X0M8_HU MAN Complement component C7 OS=Homo sapiens GN=C7 PE=1 SV=2 Neural cell adhesion molecule L1-like protein OS=Homo sapiens GN=CHL1 PE=1 SV=1 NYTLTGR Yes IIPSNNSGTFR

55 Table S7. Detailed information of the observed N-glycopeptides obtained from tryptic digests of rat brain. Data were searched against the Uniprot rat reference proteome database. In the Accessions and Names columns, the proteins in a Protein Group were listed together, and the residue numbers of N-glycosylation sites on the first protein of each Protein Group was listed in the column Residue sites. Accessions Names Sequences Scores tr G3V928 G3V928_R tr G3V928 G3V928_R tr G3V928 G3V928_R tr G3V928 G3V928_R tr G3V928 G3V928_R tr G3V928 G3V928_R tr G3V928 G3V928_R tr G3V928 G3V928_R tr G3V928 G3V928_R Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Residue sites CNASSQFLCSSGR LTSCNASMCGDEAR LYWTDGDNISMVNMDGSNR MHLNGSNVQVLHR DNDSVPLR FGTCSQLCNNTK FNSTEYQVVTR IETILLNGTDR KLNLDGSNYTLLK Uniprot notes 55

56 tr G3V928 G3V928_R tr G3V928 G3V928_R tr G3V928 G3V928_R tr G3V928 G3V928_R tr G3V928 G3V928_R tr G3V928 G3V928_R tr G3V928 G3V928_R tr G3V928 G3V928_R tr G3V928 G3V928_R tr G3V928 G3V928_R tr G3V928 G3V928_R tr G3V928 G3V928_R Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 Protein Lrp1 OS=Rattus norvegicus GN=Lrp1 LHNDNTTCYEFK LNGTDPIVAVDSK LNLDGSNYTLLK LYWISSGNHTINR NSTTLVMHMK QTGDVTCNCTDGR RGCKDNDSVPLR TCPLDEFQCNNTLCKPLAWK TCVSNCTASQFVCK GCKDNDSVPLR WTGHNVTVVQR TCPLDEFQCNNTLCKPLAWK

57 sp P97685 NFASC_R; tr R9PY05 R9PY05_R; sp P97685 NFASC_R; tr R9PY05 R9PY05_R; sp P97685 NFASC_R; tr R9PY05 R9PY05_R; sp P97685 NFASC_R; tr R9PY05 R9PY05_R; sp P97685 NFASC_R; tr R9PY05 R9PY05_R; sp P97685 NFASC_R; tr R9PY05 R9PY05_R; sp P97685 NFASC_R; tr R9PY05 R9PY05_R; sp P97685 NFASC_R; tr R9PY05 R9PY05_R; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; ITNVSEEDSGEYFCLASNK Yes QMVENFSPNQTK Yes ETWNNVTVWGSR Yes HNFRPGTDFVVEYIDSNHTK Yes HNFRPGTDFVVEYIDSNHTKK Yes YVPFNGTK Yes WANITWK Yes ETWNNVTVWGSR Yes 57

58 sp P97685 NFASC_R; tr R9PY05 R9PY05_R; tr F1MAA7 F1MAA7_R tr F1MAA7 F1MAA7_R tr F1MAA7 F1MAA7_R tr F1MAA7 F1MAA7_R tr F1MAA7 F1MAA7_R tr F1MAA7 F1MAA7_R tr F1MAA7 F1MAA7_R tr F1MAA7 F1MAA7_R sp P97686 NRCAM_R; tr Q6PW39 Q6PW39_R; tr A0A0G2K4Z2 A0A0G2K4Z2_R ; tr A0A0G2JW27 A0A0G2JW27_R ; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; Neurofascin OS=Rattus norvegicus GN=Nfasc PE=1 SV=2; Protein Lamc1 OS=Rattus norvegicus GN=Lamc1 Protein Lamc1 OS=Rattus norvegicus GN=Lamc1 Protein Lamc1 OS=Rattus norvegicus GN=Lamc1 Protein Lamc1 OS=Rattus norvegicus GN=Lamc1 Protein Lamc1 OS=Rattus norvegicus GN=Lamc1 Protein Lamc1 OS=Rattus norvegicus GN=Lamc1 Protein Lamc1 OS=Rattus norvegicus GN=Lamc1 Protein Lamc1 OS=Rattus norvegicus GN=Lamc1 Neuronal cell adhesion molecule OS=Rattus norvegicus GN=Nrcam PE=1 SV=2; Neuronal cell adhesion molecule OS=Rattus norvegicus GN=Nrcam ; Neuronal cell adhesion molecule OS=Rattus norvegicus GN=Nrcam ; Neuronal cell adhesion molecule OS=Rattus norvegicus GN=Nrcam KNNMEITWTPMNSAFGPN LR Yes VNNSLHSQISR VNNSLHSQISR LLNNLTSIK TANETSAEAYNLLLR TLAGENQTALEIEELNR TLAGENQTALEIEELNR CDQCEENYFYNR AESANQCLPCDCNGR DGDDEWTSVVVANVSK Yes 58