SUPPLEMENTAL TABLE I. Identified Proteins in Bovine Testicular Hyaluronidase Type I-S via LC-MS/MS

Transcription

1 SUPPLEMENTAL TABLE I. Identified Proteins in Bovine Testicular Hyaluronidase Type I-S via LC-MS/MS No. Protein 1 serum albumin precursor gi annexin A2 gi Phosphatidylethanolamine-binding protein 1(PEBP-1) gi glutathione S-transferase P gi Malate dehydrogenase gi serotransferrin precursor gi annexin I gi 74 8 fatty acid-binding protein, epidermal gi regucalcin gi glutathione S-transferase Mu 1 gi cathepsin D gi beta-2-glycoprotein I gi 6 13 superoxide dismutase [Cu-Zn] gi glutathione S-transferase mu 3 gi ectonucleotide pyrophosphatase/phosphodiesterase 5 gi (putative function) 16 Sperm adhesion molecule 1 (PH-20 hyaluronidase, zona gi pellucida binding) 17 acylphosphatase-1 gi acyl-coa-binding protein gi protein DJ-1 gi sialate O-acetylesterase gi Transaldolase gi leucine-rich alpha-2-glycoprotein gi alpha 3 type VI collagen, partial gi coactosin-like protein gi inter-alpha (globulin) inhibitor H4 (plasma Kallikreinsensitive gi glycoprotein) 26 glucosamine 6-phosphate N-acetyltransferase gi transgelin gi haptoglobin precursor gi plasma glutamate carboxypeptidase precursor gi epididymal secretory protein E1 precursor gi lumican precursor gi lysosomal Pro-X carboxypeptidase precursor gi annexin A5 gi rho GDP-dissociation inhibitor 1 gi acrosin gi cathepsin C gi rho GDP-dissociation inhibitor 2 gi glutathione S-transferase mu 1 gi N-acetylglucosamine-6-sulfatase precursor gi Huang et al., SUPPLEMENTAL TABLE I Page 1 of 2

2 No. Protein 40 alpha-1b-glycoprotein precursor gi SH3 domain-binding glutamic acid-rich-like protein gi annexin A3 gi alpha-1-acid glycoprotein precursor gi thioredoxin gi tubulin polymerization-promoting protein family gi member 2 46 Ig heavy chain precursor (B/MT.4A.17.H5.A5) - bovine gi immunoglobulin lambda light chain constant region 2 gi allotypic variant IGLC2a 48 cathepsin B precursor gi fatty acid-binding protein, adipocyte gi plastin-3 gi hemoglobin subunit beta gi phospholipase D3 gi UV excision repair protein RAD23 homolog B gi IGL@ gi putative phospholipase B-like 2 precursor gi kininogen I precursor gi microtubule-associated protein RP/EB family member 1 gi CD5 gi eppin gi PDZ and LIM domain protein 3 gi complement factor B precursor gi Y-box-binding protein 2 gi D-dopachrome decarboxylase gi eukaryotic translation initiation factor 3 subunit J gi macrophage migration inhibitory factor gi NAGA protein gi gamma-glutamylcyclotransferase gi Huang et al., SUPPLEMENTAL TABLE I Page 2 of 2

3 SUPPLEMENTAL TABLE II. Identified Proteins in Bovine Testicular Hyaluronidase Type IV-S via LC-MS/MS No. Protein 1 serum albumin precursor gi annexin A2 gi annexin I gi 74 4 Phosphatidylethanolamine-binding protein 1(PEBP-1) gi glutathione S-transferase P gi ectonucleotide pyrophosphatase/phosphodiesterase 5 gi (putative function) 7 cathepsin D gi beta-2-glycoprotein I gi 6 9 sialate O-acetylesterase gi fatty acid-binding protein, epidermal gi annexin A5 gi ANXA5 protein gi regucalcin gi acyl-coa-binding protein gi Sperm adhesion molecule 1 (PH-20 hyaluronidase, zona gi pellucida binding) 16 superoxide dismutase [Cu-Zn] gi N-acetylglucosamine-6-sulfatase precursor gi alpha 3 type VI collagen, partial gi cathepsin C gi putative phospholipase B-like 2 precursor gi glutathione S-transferase Mu 1 gi haptoglobin precursor gi leucine-rich alpha-2-glycoprotein gi lysosomal Pro-X carboxypeptidase precursor gi acylphosphatase-1 gi annexin A3 gi inter-alpha (globulin) inhibitor H4 (plasma Kallikreinsensitive gi glycoprotein) 28 transaldolase gi acrosin gi SH3 domain-binding glutamic acid-rich-like protein gi Malate dehydrogenase gi cathepsin B precursor gi glucosamine 6-phosphate N-acetyltransferase gi fatty acid-binding protein, heart gi kda glucose-regulated protein precursor gi phospholipase D3 gi plasma glutamate carboxypeptidase precursor gi tubulin polymerization-promoting protein family gi member 2 Huang et al., SUPPLEMENTAL TABLE II Page 1 of 3

4 No. Protein 39 coactosin-like protein gi Rsb-66 protein gi serotransferrin precursor gi epididymal secretory protein E1 precursor gi lumican precursor gi gamma-glutamylcyclotransferase gi gamma-glutamyl hydrolase precursor gi fatty acid-binding protein, adipocyte gi myosin light chain kinase, smooth muscle gi cysteine-rich secretory protein 2 gi IgG2a heavy chain constant region gi glutathione S-transferase mu 1 gi immunoglobulin lambda-like polypeptide 1-like gi alpha-1-acid glycoprotein precursor gi aminoacyl trna synthase complex-interacting gi multifunctional protein 1 54 talin 1 gi microtubule-associated protein RP/EB family member 1 gi protein NipSnap homolog 3A gi activated RNA polymerase II transcriptional coactivator gi p15 58 UV excision repair protein RAD23 homolog B gi anti-testosterone antibody gi glutathione S-transferase mu 3 gi peroxiredoxin-4 gi lymphocyte cytosolic protein 1 (L-plastin) gi phosphatidylethanolamine-binding protein 4 gi cathepsin L2 precursor gi alpha-1b-glycoprotein precursor gi cathepsin F gi heat shock-related 70 kda protein 2 gi pro-cathepsin H precursor gi plastin-3 gi heat shock cognate 71 kda protein gi complement factor B precursor gi thioredoxin gi spectrin beta chain, brain 1 gi rho GDP-dissociation inhibitor 1 gi mesencephalic astrocyte-derived neurotrophic factor gi precursor 76 palmitoyl-protein thioesterase 1 precursor gi cathepsin S gi protein DJ-1 gi LIM protein gi glucosylceramidase precursor gi Huang et al., SUPPLEMENTAL TABLE II Page 2 of 3

5 No. Protein 81 DNAJA4 protein gi interleukin enhancer binding factor 3, 90kDa gi ADP-ribosyltransferase 3 gi filamin A, alpha gi D-tyrosyl-tRNA(Tyr) deacylase 1 gi PDIA6 protein gi ganglioside GM2 activator gi Chain I, Trypsin gi immunoglobulin light chain variable region gi myristoyl CoA:protein N-myristoyltransferase gi gamma-inducible protein 30 gi lysozyme-like protein 1 precursor gi AMBP protein gi immunoglobulin lambda light chain constant region 3 gi allotypic variant IGLC3c 95 rho GDP-dissociation inhibitor 2 gi eppin gi serine carboxypeptidase vitellogenic-like gi CD5 gi tubulin polymerization-promoting protein family gi member vesicle-associated membrane protein-associated protein gi A 101 acid sphingomyelinase-like phosphodiesterase 3a gi precursor Huang et al., SUPPLEMENTAL TABLE II Page 3 of 3

6 SUPPLEMENTAL TABLE III. Identified Proteins in rhuph20 via LC-MS/MS No. Protein 1 hyaluronidase PH-20 isoform 2 [Homo sapiens] gi Huang et al., SUPPLEMENTAL TABLE I Page 1 of 1