Highly Efficient Enrichment of N-linked Glycopeptides using a Hydrophilic

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1 Electronic Supplementary Material (ESI) for Analyst. This journal is The Royal Society of Chemistry 2017 Supporting Information Highly Efficient Enrichment of N-linked Glycopeptides using a Hydrophilic Covalent-Organic Framework Yu-Fang Ma, Fang Yuan, Xiao-Hui Zhang, Ying-Lin Zhou* and Xin-Xiang Zhang* Beijing National Laboratory for Molecular Sciences (BNLMS), MOE Key Laboratory of Bioorganic Chemistry and Molecular Engineering, College of Chemistry, Peking University, Beijing , China. Corresponding Authors addresses: zhouyl@pku.edu.cn, zxx@pku.edu.cn; Fax: ; Tel: S1

2 OH HMTA,TFA 3M HCl OHC OH CHO HO OH 100 C 2.5 h, under nitrogen 100 C 1 h, under nitrogen HO CHO OH dried phloroglucinol 1, 3, 5-triformylphloroglucinol Scheme S1 Schematic representation of the synthesis of 1, 3, 5-triformylphloroglucinol (Tp). OHC HO OH CHO CHO OH NH 2 NH 2 Schiff base reaction N HO N OH OH N NH O HN O O N H Tp Pa-1 Enlo form Keto form Scheme S2 Schematic representation of the synthesis of TpPa-1. Fig. S1 1 H NMR spectrum of synthesized Tp. S2

3 Fig. S2 13 C NMR spectrum of synthesized Tp. Fig. S3 FT-IR spectra of (A) synthesized TpPa-1, (B) Pa-1 and (C) synthesized Tp. S3

4 Fig. S4 XRD patterns of (A) synthesized TpPa-1 and (B) simulated TpPa-1. Fig. S5 SEM images of synthesized TpPa-1. S4

5 Fig. S6 MALDI-TOF MS spectra of human IgG tryptic digests after TpPa-1 enrichment with different elution buffers (A) ACN/H 2 O/TFA (79.9/20/0.1, v/v/v), (B) ACN/H 2 O/TFA (84.9/15/0.1, v/v/v) and (C) ACN/H 2 O/TFA (89.9/10/0.1, v/v/v). N-linked glycopeptides are marked with solid red circles. S5

6 Fig. S7 MALDI-TOF MS spectra of human IgG tryptic digests after enrichment with TpPa-1 (A) for the third time, (B) for the sixth time and (C) for the tenth time. N-linked glycopeptides are marked with solid red circles. S6

7 Table S1 Observed molecular masses of N-linked glycopeptides and proposed glycan composition of human IgG tryptic digests enriched by TpPa-1. Hex, HexNAc, Fuc and NeuAc are the abbreviations of hexose, N- acetylhexosamine, fucose and N-acetylneuraminic acid, respectively. N# denotes the N-linked glycosylation sites. Peak number Observed m/z Glycan composite Peptide sequence I [Hex]3[HexNAc]3[Fuc]1 EEQFN#STFR I [Hex]3[HexNAc]3[Fuc]1 EEQYN#STYR I [Hex]3[HexNAc]4 EEQFN#STFR I [Hex]3[HexNAc]4 EEQYN#STYR I [Hex]4[HexNAc]3[Fuc]1 EEQFN#STFR I [Hex]4[HexNAc]3[Fuc]1 EEQYN#STYR I [Hex]3[HexNAc]4[Fuc]1 EEQFN#STFR I [Hex]4[HexNAc]4 EEQFN#STFR I [Hex]3[HexNAc]4[Fuc]1 EEQYN#STYR I [Hex]4[HexNAc]4 EEQYN#STYR I [Hex]3[HexNAc]5 EEQFN#STFR I [Hex]3[HexNAc]5 EEQYN#STYR I [Hex]4[HexNAc]4[Fuc]1 EEQFN#STFR I [Hex]5[HexNAc]4 EEQFN#STFR I [Hex]4[HexNAc]4[Fuc]1 EEQYN#STYR I [Hex]3[HexNAc]5[Fuc]1 EEQFN#STYR I [Hex]5[HexNAc]4 EEQYN#STFR I [Hex]4[HexNAc]5 EEQFN#STFR I [Hex]3[HexNAc]5[Fuc]1 EEQYN#STYR I [Hex]4[HexNAc]5 EEQYN#STYR I [Hex]4[HexNAc]4 [NeuAc]1 EEQFN#STFR I [Hex]5[HexNAc]4[Fuc]1 EEQFN#STFR I [Hex]5[HexNAc]4[Fuc]1 EEQYN#STYR I [Hex]4[Hex7NAc]5[Fuc]1 EEQFN#STFR I [Hex]5[HexNAc]5 EEQFN#STFR I [Hex]4[HexNAc]5[Fuc]1 EEQYN#STYR I [Hex]5[HexNAc]5 EEQYN#STYR I [Hex]4[HexNAc]4[Fuc]1[NeuAc]1 EEQFN#STFR S7

8 I [Hex]5[HexNAc]5[Fuc]1 EEQFN#STFR I [Hex]5[HexNAc]5[Fuc]1 EEQYN#STYR I [Hex]5[HexNAc]4[Fuc]1[NeuAc]1 EEQFN#STFR I [Hex]5[HexNAc]4[Fuc]1[NeuAc]1 EEQYN#STYR Table S2 Observed molecular masses of N-linked glycopeptides and proposed glycan composition of HRP tryptic digests enriched by TpPa-1. Hex, HexNAc, Fuc and Xyl are the abbreviations of hexose, N-acetylhexosamine, fucose and xylose, respectively. N# denotes the N-linked glycosylation sites. Peak Observed number m/z Glycan composite Peptide sequence H [Hex]3[HexNAc]2[Fuc]1[Xyl]1 NVGLN#R H [Hex]3[HexNAc]2[Fuc]1[Xyl]1 PNVSN#IVR H [Hex]3[HexNAc]2[Fuc]1[Xyl]1 SSPN#ATDTIPLVR H [Hex]3[HexNAc]2[Fuc]1[Xyl]1 PTLN#TTYLQTLR H [Hex]3[HexNAc]2[Xyl]1 MGN#ITPLTGTQGQIR H [Hex]2[HexNAc]2 MGN#ITPLTGTQGQIRLNCR H [HexNAc]1[Fuc]1 LHFHDCFVNGCDASILLDN#TTSFR H [Hex]3[HexNAc]2[Fuc]1[Xyl]1 GLCPLNGN#LSALVDFDLR H [HexNAc]1[Fuc]1 LHFHDCFVNGCDASILLDN#TTSFR H [Hex]3[HexNAc]2[Xyl]1 SFAN#STQTFFNAFVEAMDR H [Hex]3[HexNAc]2[Fuc]1[Xyl]1 QLTPTFYDNSCPN#VSNIVR H [Hex]3[HexNAc]2[Fuc]1[Xyl]1 SFAN#STQTFFNAFVEAMDR H [Hex]3[HexNAc]2[Fuc]1[Xyl]1 SFAN#STQTFFNAFNEAM*DR H [Hex]2[HexNAc]2[Fuc]1 GLIQSDQELFSSPN#ATDTIPLVR H [Hex]3[HexNAc]2[Xyl]1 GLIQSDQELFSSPN#ATDTIPLVR H [Hex]3[HexNAc]2[Fuc]1[Xyl]1 GLIQSDQELFSSPN#ATDTIPLVR H [Hex]2[HexNAc]2 LHFHDCFVNGCDASILLDN#TTSFR TEK H [Hex]3[HexNAc]2[Fuc]1[Xyl]1 LHFHDCFVNGCDASILLDN#TTSFR H [Hex]3[HexNAc]2[Fuc]1 LYN#FSNTGLPDPTLN#TTYLQTLR [Hex]3[HexNAc]2[Fuc]1 H [Hex]3[HexNAc]2[Fuc]1[Xyl]1 [Hex]3[HexNAc]2[Xyl]1 LYN#FSNTGLPDPTLN#TTYLQTLR S8

9 H [Hex]3[HexNAc]2[Fuc]1[Xyl]1 [Hex]3[HexNAc]2[Fuc]1 H [Hex]3[HexNAc]2[Fuc]1[Xyl]1 [Hex]3[HexNAc]2[Fuc]1[Xyl]1 LYN#FSNTGLPDPTLN#TTYLQTLR LYN#FSNTGLPDPTLN#TTYLQTLR Table S3 Observed N-linked glycopeptides of human serum tryptic digests enriched by TpPa-1. OS=Organism Name, GN=Gene Name, PE=Protein Existence, SV=Sequence Version. N# denotes the N-linked glycosylation sites. Protein Accession Number FETUA_HUMAN FETUA_HUMAN FETUA_HUMAN IGHG1_HUMAN A2MG_HUMAN A2MG_HUMAN A2MG_HUMAN A1AT_HUMAN Protein Description Alpha-2-HS-glycoprotein OS=Homo sapiens GN=AHSG Alpha-2-HS-glycoprotein OS=Homo sapiens GN=AHSG Alpha-2-HS-glycoprotein OS=Homo sapiens GN=AHSG Ig gamma-1 chain C region OS=Homo sapiens GN=IGHG1 Alpha-2-macroglobulin OS=Homo sapiens GN=A2M Alpha-2-macroglobulin OS=Homo sapiens GN=A2M Alpha-2-macroglobulin OS=Homo sapiens GN=A2M Alpha-1-antitrypsin OS=Homo sapiens GN=SERPINA1 PE=1 Peptide Sequence VCQDCPLLAPLN#DTR KVCQDCPLLAPLN#DTR AALAAFNAQNN#GSNFQLEEISR EEQYN#STYR VSN#QTLSLFFTVLQDVPVR GCVLLSYLN#ETVTVSASLESVR SLGNVN#FTVSAEALESQELCGTEVPSVPEH GR YLGN#ATAIFFLPDEGK S9

10 SV=3 A1AT_HUMAN Alpha-1-antitrypsin OS=Homo ADTHDEILEGLNFN#LTEIPEAQIHEGFQELL sapiens GN=SERPINA1 PE=1 R SV=3 CO3_HUMAN Complement C3 OS=Homo TVLTPATNHMGN#VTFTIPANR sapiens GN=C3 HEMO_HUMAN Hemopexin OS=Homo sapiens SWPAVGN#CSSALR GN=HPX HEMO_HUMAN Hemopexin OS=Homo sapiens ALPQPQN#VTSLLGCTH GN=HPX HEMO_HUMAN Hemopexin OS=Homo sapiens N#GTGHGN#STHHGPEYMR GN=HPX IGHG4_HUMAN Ig gamma-4 chain C region EEQFN#STYR OS=Homo sapiens GN=IGHG4 IGHG2_HUMAN Ig gamma-2 chain C region EEQFN#STFR OS=Homo sapiens GN=IGHG2 IGHG3_HUMAN Ig gamma-3 chain C region OS=Homo sapiens GN=IGHG3 EEQYN#STFR CERU_HUMAN Ceruloplasmin OS=Homo EHEGAIYPDN#TTDFQR sapiens GN=CP CERU_HUMAN Ceruloplasmin OS=Homo ELHHLQEQN#VSNAFLDK sapiens GN=CP CERU_HUMAN Ceruloplasmin OS=Homo EN#LTAPGSDSAVFFEQGTTR sapiens GN=CP CERU_HUMAN Ceruloplasmin OS=Homo ELHHLQEQN#VSNAFLDKGEFYIGSK sapiens GN=CP CLUS_HUMAN Clusterin OS=Homo sapiens HN#STGCLR GN=CLU CLUS_HUMAN Clusterin OS=Homo sapiens EDALN#ETR GN=CLU CLUS_HUMAN Clusterin OS=Homo sapiens KEDALN#ETR S10

11 GN=CLU CLUS_HUMAN Clusterin OS=Homo sapiens GN=CLU CLUS_HUMAN Clusterin OS=Homo sapiens GN=CLU IGHA2_HUMAN Ig alpha-2 chain C region OS=Homo sapiens GN=IGHA2 PE=1 SV=3 IGHA2_HUMAN Ig alpha-2 chain C region OS=Homo sapiens GN=IGHA2 PE=1 SV=3 IGHA1_HUMAN Ig alpha-1 chain C region OS=Homo sapiens GN=IGHA1 IGHA1_HUMAN Ig alpha-1 chain C region OS=Homo sapiens GN=IGHA1 A1AG1_HUMAN Alpha-1-acid glycoprotein 1 OS=Homo sapiens GN=ORM1 A1AG1_HUMAN Alpha-1-acid glycoprotein 1 OS=Homo sapiens GN=ORM1 ANT3_HUMAN Antithrombin-III OS=Homo sapiens GN=SERPINC1 PE=1 SV=1 ANT3_HUMAN Antithrombin-III OS=Homo sapiens GN=SERPINC1 PE=1 SV=1 ANT3_HUMAN Antithrombin-III OS=Homo sapiens GN=SERPINC1 PE=1 SV=1 ANT3_HUMAN Antithrombin-III OS=Homo sapiens GN=SERPINC1 PE=1 KKEDALN#ETR LAN#LTQGEDQYYLR TPLTAN#ITK LSLHRPALEDLLLGSEAN#LTCTLTGLR LAGKPTHVN#VSVVMAEVDGTCY LSLHRPALEDLLLGSEAN#LTCTLTGLR EN#GTISR QDQCIYN#TTYLNVQR WVSN#KTEGR LGACN#DTLQQLMEVFK SLTFN#ETYQDISELVYGAK LGACN#DTLQQLMEVFKFDTISEK S11

12 SV=1 ZA2G_HUMAN Zinc-alpha-2-glycoprotein OS=Homo sapiens GN=AZGP1 HPT_HUMAN Haptoglobin OS=Homo sapiens GN=HP HPT_HUMAN Haptoglobin OS=Homo sapiens GN=HP HPT_HUMAN Haptoglobin OS=Homo sapiens GN=HP APOB_HUMAN Apolipoprotein B-100 OS=Homo sapiens GN=APOB APOB_HUMAN Apolipoprotein B-100 OS=Homo sapiens GN=APOB APOB_HUMAN Apolipoprotein B-100 OS=Homo sapiens GN=APOB APOB_HUMAN Apolipoprotein B-100 OS=Homo sapiens GN=APOB APOB_HUMAN Apolipoprotein B-100 OS=Homo sapiens GN=APOB APOB_HUMAN Apolipoprotein B-100 OS=Homo sapiens GN=APOB IGHM_HUMAN Ig mu chain C region OS=Homo sapiens GN=IGHM PE=1 SV=3 IGHM_HUMAN Ig mu chain C region OS=Homo sapiens GN=IGHM PE=1 SV=3 IGHM_HUMAN Ig mu chain C region OS=Homo sapiens GN=IGHM PE=1 SV=3 DIVEYYN#DSN#GSHVLQGR NLFLN#HSEN#ATAK VVLHPN#YSQVDIGLIK MVSHHN#LTTGATLINEQWLLTTAK YDFN#SSMLYSTAK FVEGSHN#STVSLTTK FN#SSYLQGTNQITGR VNQNLVYESGSLN#FSK FEVDSPVYN#ATWSASLK QVFPGLNYCTSGAYSN#ASSTDSASYYPLTG DTR N#NSDISSTR YKN#NSDISSTR GLTFQQN#ASSMCVPDQDTAIR S12

13 IGHM_HUMAN Ig mu chain C region OS=Homo sapiens GN=IGHM PE=1 SV=3 VTNC_HUMAN Vitronectin OS=Homo sapiens GN=VTN CO4A_HUMAN Complement C4-A OS=Homo sapiens GN=C4A CO4A_HUMAN Complement C4-A OS=Homo sapiens GN=C4A APOH_HUMAN Beta-2-glycoprotein 1 OS=Homo sapiens GN=APOH PE=1 SV=3 APOH_HUMAN Beta-2-glycoprotein 1 OS=Homo sapiens GN=APOH PE=1 SV=3 AACT_HUMAN Alpha-1-antichymotrypsin OS=Homo sapiens GN=SERPINA3 AACT_HUMAN Alpha-1-antichymotrypsin OS=Homo sapiens GN=SERPINA3 HRG_HUMAN Histidine-rich glycoprotein OS=Homo sapiens GN=HRG CFAH_HUMAN Complement factor H OS=Homo sapiens GN=CFH PE=1 SV=4 CFAH_HUMAN Complement factor H OS=Homo sapiens GN=CFH PE=1 SV=4 CFAH_HUMAN Complement factor H OS=Homo sapiens GN=CFH PE=1 SV=4 A1AG2_HUMAN Alpha-1-acid glycoprotein 2 OS=Homo sapiens GN=ORM2 THTN#ISESHPN#ATFSAVGEASICEDDWNS GER NN#ATVHEQVGGPSLTSDLQAQSK GLN#VTLSSTGR FSDGLESN#SSTQFEVK LGN#WSAMPSCK VYKPSAGN#NSLYR YTGN#ASALFILPDQDK FN#LTETSEAEIHQSFQHLLR VIDFN#CTTSSVSSALANTK MDGASN#VTCINSR ISEEN#ETTCYMGK IPCSQPPQIEHGTIN#SSR EN#GTVSR S13

14 A1AG2_HUMAN Alpha-1-acid glycoprotein 2 OS=Homo sapiens GN=ORM2 CBG_HUMAN Corticosteroid-binding globulin OS=Homo sapiens GN=SERPINA6 KAIN_HUMAN Kallistatin OS=Homo sapiens GN=SERPINA4 PE=1 SV=3 KAIN_HUMAN Kallistatin OS=Homo sapiens GN=SERPINA4 PE=1 SV=3 KNG1_HUMAN Kininogen-1 OS=Homo sapiens GN=KNG1 KNG1_HUMAN Kininogen-1 OS=Homo sapiens GN=KNG1 C4BPA_HUMAN C4b-binding protein alpha chain OS=Homo sapiens GN=C4BPA APOD_HUMAN Apolipoprotein D OS=Homo sapiens GN=APOD APOD_HUMAN Apolipoprotein D OS=Homo sapiens GN=APOD FINC_HUMAN Fibronectin OS=Homo sapiens GN=FN1 PE=1 SV=3 FINC_HUMAN Fibronectin OS=Homo sapiens GN=FN1 PE=1 SV=3 ITIH4_HUMAN Inter-alpha-trypsin inhibitor heavy chain H4 OS=Homo sapiens GN=ITIH4 PE=1 SV=4 LG3BP_HUMAN Galectin-3-binding protein OS=Homo sapiens GN=LGALS3BP LG3BP_HUMAN Galectin-3-binding protein OS=Homo sapiens GN=LGALS3BP QNQCFYN#SSYLNVQR AQLLQGLGFN#LTER DFYVDEN#TTVR SQILEGLGFN#LTELSESDVHR ITYSIVQTN#CSK LNAENN#ATFYFK LSVDKDQYVEPEN#VTIQCDSGYGVVGPQS ITCSGNR ADGTVNQIEGEATPVN#LTEPAK ADGTVNQIEGEATPVN#LTEPAKLEVK DQCIVDDITYNVN#DTFHK LDAPTNLQFVN#ETDSTVLVR LPTQN#ITFQTESSVAEQEAEFQSPK DAGVVCTN#ETR AAIPSALDTN#SSK S14

15 LG3BP_HUMAN Galectin-3-binding protein OS=Homo sapiens GN=LGALS3BP LG3BP_HUMAN Galectin-3-binding protein OS=Homo sapiens GN=LGALS3BP BTD_HUMAN Biotinidase OS=Homo sapiens GN=BTD BTD_HUMAN Biotinidase OS=Homo sapiens GN=BTD AFAM_HUMAN Afamin OS=Homo sapiens GN=AFM AFAM_HUMAN Afamin OS=Homo sapiens GN=AFM ATRN_HUMAN Attractin OS=Homo sapiens GN=ATRN ATRN_HUMAN Attractin OS=Homo sapiens GN=ATRN ATRN_HUMAN Attractin OS=Homo sapiens GN=ATRN KLKB1_HUMAN Plasma kallikrein OS=Homo sapiens GN=KLKB1 KLKB1_HUMAN Plasma kallikrein OS=Homo sapiens GN=KLKB1 KLKB1_HUMAN Plasma kallikrein OS=Homo sapiens GN=KLKB1 IGJ_HUMAN Immunoglobulin J chain OS=Homo sapiens GN=IGJ PE=1 SV=4 FHR1_HUMAN Complement factor H-related protein 1 OS=Homo sapiens GN=CFHR1 C4BPB_HUMAN C4b-binding protein beta chain OS=Homo sapiens GN=C4BPB ALGFEN#ATQALGR GLN#LTEDTYKPR FN#DTEVLQR NPVGLIGAEN#ATGETDPSHSK DIENFN#STQK YAEDKFN#ETTEK GICN#SSDVR IDSTGN#VTNELR N#HSCSEGQISIFR GVNFN#VSK IYSGILN#LSDITK IYPGVDFGGEELN#VTFVK EN#ISDPTSPLR LQNNENN#ISCVER EWDN#TTTECR S15

16 C4BPB_HUMAN C4b-binding protein beta chain OS=Homo sapiens GN=C4BPB MUCB_HUMAN Ig mu heavy chain disease protein OS=Homo sapiens CFAI_HUMAN Complement factor I OS=Homo sapiens GN=CFI CFAI_HUMAN Complement factor I OS=Homo sapiens GN=CFI PON1_HUMAN Serum paraoxonase/arylesterase 1 OS=Homo sapiens GN=PON1 PON1_HUMAN Serum paraoxonase/arylesterase 1 OS=Homo sapiens GN=PON1 PEDF_HUMAN Pigment epithelium-derived factor OS=Homo sapiens GN=SERPINF1 PE=1 SV=3 CO9_HUMAN Complement component C9 OS=Homo sapiens GN=C9 PE=1 SV=2 LUM_HUMAN Lumican OS=Homo sapiens GN=LUM LUM_HUMAN Lumican OS=Homo sapiens GN=LUM LUM_HUMAN Lumican OS=Homo sapiens GN=LUM CPN2_HUMAN Carboxypeptidase N subunit 2 OS=Homo sapiens GN=CPN2 CPN2_HUMAN Carboxypeptidase N subunit 2 OS=Homo sapiens GN=CPN2 LGHCPDPVLVNGEFSSSGPVN#VSDK THTN#ISESHPN#ATFSAVGEASICEDDWDS GER FLNN#GTCTAEGK LSDLSIN#STECLHVHCR HAN#WTLTPLK VTQVYAEN#GTVLQGSTVASVYK VTQN#LTLIEESLTSEFIHDIDR AVN#ITSENLIDDVVSLIR LHINHNN#LTESVGPLPK LGSFEGLVN#LTFIHLQHNR AFEN#VTDLQWLILDHNLLENSK AFGSNPN#LTK LYLGSNN#LTALHPALFQN#LSK S16

17 IBP3_HUMAN Insulin-like growth factorbinding protein 3 OS=Homo sapiens GN=IGFBP3 THRB_HUMAN Prothrombin OS=Homo sapiens GN=F2 THRB_HUMAN Prothrombin OS=Homo sapiens GN=F2 THRB_HUMAN Prothrombin OS=Homo sapiens GN=F2 C1QA_HUMAN Complement C1q subcomponent subunit A OS=Homo sapiens GN=C1QA TSP1_HUMAN Thrombospondin-1 OS=Homo sapiens GN=THBS1 HEP2_HUMAN Heparin cofactor 2 OS=Homo sapiens GN=SERPIND1 PE=1 SV=3 SEPP1_HUMAN Selenoprotein P OS=Homo sapiens GN=SEPP1 PE=1 SV=3 CHP1_HUMAN Calcium-binding protein p22 OS=Homo sapiens GN=CHP PE=1 SV=3 IGHD_HUMAN Ig delta chain C region OS=Homo sapiens GN=IGHD CHLE_HUMAN Cholinesterase OS=Homo sapiens GN=BCHE PHLD_HUMAN Phosphatidylinositol-glycanspecific phospholipase D OS=Homo sapiens GN=GPLD1 PE=1 SV=3 GLCVN#ASAVSR GHVN#ITR N#FTENDLLVR YPHKPEIN#STTHPGADLQENFCR RNPPMGGNVVIFDTVITNQEEPYQN#HSG R VVN#STTGPGEHLR DFVN#ASSK EGYSN#ISYIVVNHQGISSR GEN#GTLSR TLLN#ASR DN#NSIITR N#LTTSLTESVDR S17

18 PGRP2_HUMAN N-acetylmuramoyl-L-alanine GFGVAIVGN#YTAALPTEAALR amidase OS=Homo sapiens GN=PGLYRP2 PGRP2_HUMAN N-acetylmuramoyl-L-alanine LEPVHLQLQCMSQEQLAQVAAN#ATK amidase OS=Homo sapiens GN=PGLYRP2 APOM_HUMAN Apolipoprotein M OS=Homo TELFSSSCPGGIMLN#ETGQGYQR sapiens GN=APOM LPAL2_HUMAN Apolipoprotein(a)-like protein 2 OS=Homo sapiens GN=LPAL2 PE=2 SV=1 WEYCN#LTR THBG_HUMAN Thyroxine-binding globulin TLYETEVFSTDFSN#ISAAK OS=Homo sapiens GN=SERPINA7 CO6_HUMAN Complement component C6 VLN#FTTK OS=Homo sapiens GN=C6 PE=1 SV=3 WDTC1_HUMAN VN#ITR LAMB2_HUMAN Laminin subunit beta-2 VN#LTR OS=Homo sapiens GN=LAMB2 PLTP_HUMAN Phospholipid transfer protein VSN#VSCQASVSR OS=Homo sapiens GN=PLTP FA10_HUMAN Coagulation factor X OS=Homo GDNN#LTR sapiens GN=F10 LYAM1_HUMAN L-selectin OS=Homo sapiens GN=SELL DN#YTDLVAIQNK C1S_HUMAN Complement C1s NCGVN#CSGDVFTALIGEIASPNYPKPYPEN subcomponent OS=Homo SR sapiens GN=C1S LYVE1_HUMAN Lymphatic vessel endothelial ANQQLN#FTEAK hyaluronic acid receptor 1 OS=Homo sapiens GN=LYVE1 S18

19 S19