HEALTH AND PERSONAL CARE
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2 Health and Personal Care has a product portfolio that includes fixed oils, butters, clays and complex that provides excellent quality to customers worldwide, with proven scientific benefits, unquestionable quality, efficiency and traceability. Product Code: CTA-C093P GENERAL INFORMATION SKIN PHISIOLOGY The skin is considered the largest organ of the body and has many different functions: - Thermoregulator - Protection - Metabolic function - Sensation It is divided into three main regions, each providing a distinct role in the overall function of the skin: - Epidermis - Dermis - Hypodermis
3 THE EPIDERMIS Is the most superficial layer of the skin and provides the first barrier of protection from the invasion of foreign substances into the body. The principal cell of the epidermis is called keratinocyte. The epidermis is subdivided into five layer or stratum, stratum corneum, stratum lucidum, stratum granulosum, stratum spinosum and stratum basale in which the keratinocytes gradually migrates to the surface and sloughed off in a process called desquamation.
4 THE DERMIS Is attached to an underlying hypodermis, also called subcutaneous conective tissue, which stores adipose tissue and is recognized fascia of gross anatomy. The dermis assumes the important function of thermoregulation and supports the vascular network to supply the avascular epidermis with nutrients. It`s divided in two zones, papillary dermis and reticular layer. It contains fibroblasts which are responsible for secreting collagen, elastin and other substances that give the support and elasticity of the skin. Also present are immune cells that are involved in defense against foreign invades passing through the epidermis. EPIDERMIS DERMIS TOP ENEMIES FOR SKIN You ve heard it said that by 20, you have the skin you inherited; by age 40, you have the skin you deserve; and by age 60, you have the skin you ve earned! Whether you need to maintain your skin, preserve your skin, or repair your skin, it s never too early and it s never too late. - Smoking: Smoking constricts blood vessels, restricts oxygen uptake, gives a gray color to your complexion, and literally eats up vitamin C, which is necessary for collagen formation. It leads to puckering around the mouth and fine squinty creases around your eyes.
5 - Pollution: Pollution affects your skin in the same way as smoking, minus the puckering and creasing. - Alcohol: Alcohol dehydrates you from the inside out, taxes your liver, and gobbles up your B vitamins. - Drugs: Some drugs may cause photosensitivity (sun sensitivity), dryness, blotchiness, or even mild acne. - Constipation: Toxins can build up within your body if your elimination is faulty, but they must eventually escape via some channel, and frequently that path is the skin. Drink plenty of fresh water and eat lots of fiber to keep your plumbing running smoothly. - Sunlight: Excessive sun exposure leads to dry, wrinkled, leathery, blotchy, prematurely aged skin and possibly skin cancer. You need your daily dose of vitamin D for fifteen minutes a day before 10:30 AM or after 4:30 PM. PROPERTIES BERACARE DPS DERMAL PROTECTIVE ACTIVE SYSTEM improves fibroblast functionality and protective ability against oxidative stress and inflammatory stimuli, promoting a healthier and more balanced skin due to the natural botanical extracts. Each extract has actives such as Caffeine, Catechins, Chlorogenic Acid, 6-Gingerol, Rosmarinic Acid promoting several optimal effects. The main properties are: Dermal anti-inflammatory effect Antioxidant activity (efficacy proven by the DPPH assay) Helps to protect the skin from UVA damage Helps to protect and regenerate the dermic layer Helps to increase and maintain skin elasticity
6 EFFICACY TESTS HEALTH AND PERSONAL CARE It was performed two different tests: 1- Evaluation of Antioxidant Potential by DPPH Assay 2- Evaluation of Anti-inflammatory Activity 1- EVALUATION OF ANTIOXIDANT POTENTIAL BY DPPH ASSAY The test consists in evaluate the ability of the test product (DPS) in sequester the freeradical called DPPH, simulating the real situation of a release by free radical cell. Considered a biochemical assay, for comparison was used Trolox (synthetic vitamin E), reference in antioxidant activity. From the results obtained it was determined the EC50 (concentration product required to sequester 50% of DPPH free) through this value is possible to compare with the reference value of Trolox. It is important to remember that lower the EC50 value, the capacity of antioxidant is higher. Calculated value for EC50 (mg/ml) Trolox 0,1005 Beracare DPS 1,003 The EC50 value of DPS was ten times higher than Trolox. For this result it is important to observe that the antioxidant activity must be considered for DPS even with the less potential than Vitamine E. 2- EVALUATION OF ANTI-INFLAMMATORY ACTIVITY The determination of the anti-inflammatory activity was performed by DPS quantification of the inflammatory mediator interleukin-1alpha (IL-1α). For induction of the inflammatory process and consequent induction of release IL-1α were used two different methods: 1) Irradiation UVA. Positive Control: Alpha-Bisabolol. 2) Incubation with the surfactant sodium dodecyl sulfate (SDS). Control Positive: Betamethasone. In both assays the cells were treated for 1 hour with SDS and respective positive controls, prior to induction of inflammation. The concentration of mediator was determined by immunoassay (ELISA). It was evaluated two concentrations of DPS, but the graphs presents only the concentration the highest tested.
7 The statistical test used was ANOVA followed by the comparison test Tukey multiple. The significance level of p < PROTOCOL I CONCLUSION In the induction of inflammation by UVA, DPS was effective in reducing significant release of IL-1α. The reduction was 58% compared to untreated control. The positive control Alpha-Bisabolol (a sesquiterpenic alcohol with moderate anti-inflammatory action) reduced 86% to release of IL-1α. 2- PROTOCOL II
8 CONCLUSION Similar to that observed by protocol 1, DPS was able to reduce significant release of IL-1α upon exposure of the cells to SDS (a surfactant known to be irritating, frequently used in clinical trials). In this situation the DPS was more potent than the positive control Betamethasone (corticosteroid). The betamethasone reduced mediator release by 39% and DPS reduced in 81%. APPLICATION AND COSMETIC USES According to the evaluations BERACARE DPS can be applied in cosmetic formulations with special concentration, from 0.5% mainly in products for the treatment of damage and dry skin and in anti-aging products. It can be applied in formulations like: - Lotions (W/O and O/W); - Creams (W/o and O/W); - Serums; - Soaps; - Cleansers; - Make-ups For shampoos: % (surfactant dispersible deposits can happen in a translucid base) Creams: % ph stability: 3 9 PHYSICAL-CHEMICAL SPECIFICATIONS Chemical and physical information available on the product specification sheet.
9 REGULATORY INFORMATION INCI Name (CTFA) CAFFEINE, COFFEA ARABICA (COFFEE) SEED EXTRACT, MALTODEXTRIN, MELISSA OFFICINALIS LEAF EXTRACT, POTASSIUM SORBATE, SILICA, SODIUM BENZOATE, ZINGIBER OFFICINALE (GINGER) RHIZOME EXTRACT INCI Name (EU LABELLING) CAFFEINE, COFFEA ARABICA SEED EXTRACT, MALTODEXTRIN, MELISSA OFFICINALIS LEAF EXTRACT, POTASSIUM SORBATE, SILICA, SODIUM BENZOATE, ZINGIBER OFFICINALE RHIZOME EXTRACT CAS Number / / / CAS Number / / / STORAGE INFORMATION Shelf Life 12 months Conditions Dry, cool, airy place, away from light and heat and other sources in an environment with constant temperature not exceeding 25 C. Container Original IMPORTANT OBSERVATIONS Considering that is a natural product, if the storage guidelines are not met, the physicochemical characteristics may vary, reducing their shelf life. After opening the product to be consumed as soon as possible. Contact with oxygen generates an oxidative process by decreasing the shelf-life of the product.
10 REFERENCES - Viglioglia y Rubin Cosmiatria II. 2 ª edition, Cosmetics & Toiletries magazine. Vol.119, no. 3/March Peyrefitte, G.; Martini, M.C.; Chivot, M. Cahiers D Esthétique Cosmétique. Andrei, National Health Council: Resolution 196/96 of the Ministry of Health. Official diary, Oct.16, Sampaio e Col. Dermatology. Fourthy edition, Medicals Arts, Basketter, reynolds & York: Predicitive testing in Contact Dermatitis: Irritant Dermatitis. Elsevier Science Inc, Kligman, A.M.; Wooding, W.M. A method for the measurement and Evaluation of Irritants of Human Skin. J. Invest Dermatol. 49:78-94, Maibach, H.I.; Epstein, W.L. Predicitive Patch testing for Sensitization and irritation. Am. Perf. And Cosm.80: Maibach, H.I.; Marzulli, F.N. Dermatotoxicology. Fifth Edition. Taylor and Francis Publishers, Jackson, E.M.; Goldner, R. irritant Contact Dermatitis. Marcel Dekker, INC, Walker, A.P.; Basketter, D.A.; Baverel, M. e col. Test Guidelines for the Assessment of Skin Tolerance of Potentially Irritant Cosmetic Ingredients in Man. Food and Chemical Toxicology. 35: , Rietschel R.L, Towler, J.F.J. Fisher s Contact Dermatitis. Fifth Edition. Lizriwot Williams & Williams. - Cosmetics & Toiletries magazine. Vol.119, no. 3/March Basile A.C.; Sertié J.Á.; Freitas P.C.; Zanini A.C. J Ethnopharmacol, 22:1, 1988Jan, HAMMER et al Journal of Ethnopharmacology, 40: ; P. - GRENARD et al Pharmacopés Tradicionalles en Guyane, Orstom, Paris, pp
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