Axons in the desert II

Transcription

1 Research Workshop of the Israel Science Foundation Ben-Gurion University of the Negev & Kibbutz Ein Gedi, Israel 1-4 November, 2015 Sponsored by:

2 1-4 November, 2015 Beer Sheva & Ein Gedi There has been a growing interest in various aspects of electrogenesis of central axons in both health and neurological disease. We therefore see this workshop as an opportunity to not only exchange our latest findings, but also to frankly confront controversies and try to resolve and understand some of the conflicting evidence that has arisen from studies entailing different techniques, experimental preparations and disciplines. Among the subjects we would like to address: What is the distribution of sodium channels of different subtype in soma-dendritic and axon initial segment membrane How should action potential generation be modeled and what parameters are lacking to make definitive models? What aspects of the axon initial segment are generalized to all cell types and what are specific to specific types? How do the protein-protein interactions that must occur in the complex axonal structure translate into function? What is the evidence for normal or pathogenic plasticity of the axon initial segment, what are the conditions under which this plasticity is seen, and what might be its functional consequence? How do myelination and demyelination affect action potential propagation? What is the role of the cytoskeletal elements that define the axon initial segment in establishing and maintaining functional polarity? 2

3 Mechanisms and consequences of axon initial segment innervation and modulation by GABAergic interneurons Energetics of action potential generation in the axon Relationships between action potentials and local Ca 2+ signaling in the axons How do axonal dynamic properties affect the firing properties of neurons and networks? Organizing Committee: Ilya Fleidervish, Mike Gutnick, Idan Segev, Alex Binshtok Program Committee: Ilya Fleidervish, Maarten Kole, Matthew Grubb Special thanks to Yana Khrapunsky for administrative assistance. 3

4 List of Speakers Yael Amitai Bernard Attali Alex Binshtok Romain Brette Juan Burrone Andreas Draguhn Alexei Egorov Ilya Fleidervish Dani Gitler David Golomb Matthew Grubb Mike Gutnick Stefan Hallermann John Huguenard Hanoch Kaphzan Maarten Kole Alon Korngreen Hiroshi Kuba Israel Melamed Alon Monsenego Andreas Neef Elior Peles John Rash Moran Rubinshtein Menahem Segal Idan Segev Mala Shah Maoz Shamir Yousheng Shu Maxim Volgushev Fred Wolf Yoel Yaari Tel-Aviv University, Israel The Hebrew University of Jerusalem, Israel CNRS and Université Paris Descartes, France King's College London, UK University of Heidelberg, Germany University of Heidelberg, Germany King's College London, UK The Hebrew University of Jerusalem, Israel University Leipzig, Germany Stanford University, USA University of Haifa, Israel The Netherlands Institute for Neuroscience, Netherlands Bar-Ilan University, Israel Nagoya University, Japan MPI for Dynamics and Self-Organization, Germany Weizmann Institute of Science, Israel Colorado State University, USA Tel Aviv University Weizmann Institute of Science, Israel The Hebrew University of Jerusalem, Israel University College London, UK Beijing Normal University, China University of Connecticut, USA MPI for Dynamics and Self-Organization, Germany The Hebrew University of Jerusalem, Israel

5 Conference Programme Axons in the desert II Sunday, November 1 - Beer Sheva Venue: Ilse Katz Institute for Nanoscale Science and Technology building (51, Ctrl+Click the interactive map below), room :30 Get together, light refreshments 14:45 Welcome, Introduction and Logistics Session 1: Myelinated axons: structure and function Chair: John Huguenard 15:00 Elior Peles, Weizmann Institute of Science, Israel The organization of myelinated axons - lessons from the PNS 15:45 John Rash, Colorado State University, USA Axonal KV1.1/KV1.2 delayed-rectifier channels are coupled to connexin 29 hemichannels in juxtaparanodal myelin of rodent sciatic nerve: Basis for a new K + kindling hypothesis of saltatory conduction 16:30 Maarten Kole, The Netherlands Institute for Neuroscience, Netherlands Multiple roles of oligodendroglial cells in the initiation and conduction of action potentials 17:30 Transportation to Shevadron Farm Restaurant, link (in Hebrew), map (in Hebrew) 5

6 18:00 Dinner 20:00 Transportation to Kibbutz Ein Gedi 6

7 Monday, November 2, Ein Gedi 07:00 08:45 Breakfast Session 2: Axon initial segment plasticity Chair: Yoel Yaari 9:00 9:30 Matthew Grubb, King's College London, UK Structural change at the axon initial segment and its interaction with coincident mechanisms of activity-dependent plasticity 9:30 10:00 Hiroshi Kuba, Nagoya University, Japan Switching of Kv channels enhances neuronal excitability during structural plasticity at the axon initial segment 10:00 10:30 Juan Burrone, King's College London, UK Activity-dependent plasticity of the axon initial segment and its synapses 10:30 11:00 Coffee break Session 3: Axonal action potential initiation - I Chair: Juan Burrone 11:00 11:30 Andreas Neef, MPI for Dynamics and Self-Organization, Germany The biophysics of the action potential generator 11:30 12:00 Mike Gutnick, The Hebrew University of Jerusalem, Israel TBA 12:00 12:30 Romain Brette, CNRS and Université Paris Descartes, France The compartmentalization of spike initiation 12:30 14:00 Lunch Session 4: Axonal action potential initiation - II Chair: Hiroshi Kuba 14:00 14:30 Maxim Volgushev, University of Connecticut, USA Action potential generation and encoding in cortical neurons: What theory predicts and what experiments are showing 14:30 15:00 Yousheng Shu, Beijing Normal University, China Action potential initiation in neocortical inhibitory interneurons 15:00 15:30 Ilya Fleidervish, Ben-Gurion University, Israel Inactivation properties of the axonal sodium channels 7

8 15:30 16:00 Coffee break Session 5: Axonal action potential initiation - III Chair: Fred Wolf 16:00 16:30 Stefan Hallermann, University Leipzig, Germany Axonal hyperpolarization activated currents enable high-frequency firing 16:30 17:00 Moran Rubinstein, Tel-Aviv University, Israel Dissecting the phenotypes of Dravet syndrome by gene deletion 17:00 17:30 Alexei Egorov, University of Heidelberg, Germany Diversity of axonal origin in hippocampal pyramidal neurons 17:30 19:00 Poster session I 19:00 20:30 Dinner 20:30 -- Posters, Wine & Fruits 8

9 Tuesday, November 3, Ein Gedi 07:00 08:45 Breakfast Session 6: Action potential properties and information encoding in neuronal circuits Chair: Andreas Draguhn 9:00 9:30 Fred Wolf, MPI for Dynamics and Self-Organization, Germany TBA 9:30 10:00 Idan Segev, The Hebrew University of Jerusalem, Israel TBA 10:00 10:30 Alon Korngreen, Bar-Ilan University, Israel Realistic neuronal modeling: is it realistic? 10:30 11:00 Coffee break Session 7: Axons and circuits Chair: Yousheng Shu 11:00 11:30 Andreas Draguhn, University of Heidelberg, Germany Ectopic spike generation in hippocampal networks 11:30 12:00 John Huguenard, Stanford University, USA Cell type specific Na channel gating in barrel cortex 12:00 12:30 David Golomb, Response of layer-4 circuits to somatosensory thalamic inputs 12:30 14:00 Lunch Session 8: Potassium channels and modulation of axonal excitability Chair: Menachem Segal 14:00 14:30 Bernard Attali, Tel-Aviv University, Israel Imbalance of M-channel activity leads to fast homeostatic plasticity at the axon initial segment of hippocampal neurons 14:30 15:00 Mala Shah, University College London, UK Cholinergic fibre activity-induced axonal ion channel plasticity in adult hippocampal granule cells 9

10 15:00 15:30 Yoel Yaari, The Hebrew University of Jerusalem, Israel Axon initial segment KV7 channels as targets for inflammatory processes 15:30 16:00 Coffee break Session 9: Axonal properties - general Chair: John Rash 16:00 16:30 Alex Binshtok, The Hebrew University of Jerusalem, Israel Neuronal GPS: Mapping axonal projections using multispectral labeling technique 16:30 17:00 Menahem Segal, Weizmann Institute of Science, Israel Modulation of ankyrin in the axon initial segment 17:00 17:30 Hanoch Kaphzan, University of Haifa, Israel tdcs: axonal perspective 17:30 19:00 Poster session II 19:00 20:30 Dinner 20:30 -- Posters, Wine & Fruits 10

11 Wednesday, November 4, Ein Gedi 07:00 08:00 Breakfast 8:00 12:00 Trip to Masada National Park (control + click) 12:30 13:30 Lunch 13:30 15:00 General Discussion. Moderator: TBA 16:00 Transportation to Beer Sheva 11

12 Poster Session 1 - Monday, November 2-17:00-19:00, 20:30-1. Adna Dumitrescu, King's College London, UK Live imaging of the axon initial segment development and plasticity 2. Christopher Makinson, Stanford University, USA Contribution of the voltage gated sodium channel Nav1.6 to forward and back propagation within a thalamocortical circuit 3. Maria Teleńczuk, CNRS and Université Paris Descartes, France Origin of the kink of somatic action potentials 4. Elisa Galliano, King's College London, UK Development and plasticity of axon initial segments in bulbar dopaminergic neurons 5. Jonathan Lezmy, Tel-Aviv University, Israel Imbalance of M-channel activity produces fast homeostatic plasticity at the axon initial segment of cultured hippocampal neurons 6. Ohad Stoler, Ben-Gurion University, Israel Subthreshold calcium flux in axons of L5 pyramidal neurons 7. Guy Eyal, The Hebrew University of Jerusalem, Israel Human L2/3 pyramidal cells possess unique membrane and dendritic properties with augmented computational capabilities 12

13 Poster Session 2 - Tuesday, November 3-17:30-19:00, 20:30-1. Arne Battefeld, The Netherlands Institute for Neuroscience Neuron-oligodendrocyte calcium signaling in the grey matter 2. Mustafa S. Hamada, The Netherlands Institute for Neuroscience Functional impact of axon coupling to basal dendrites in pyramidal neurons 3. Marko Popovic, The Netherlands Institute for Neuroscience Spatio-temporal dynamics of calcium flux in the axonal initial segment 4. Niklas Byczkowicz, University Leipzig, Germany Hyperpolarization-activated currents in cerebellar mossy fibers 5. Robert Goldstein, The Hebrew University of Jerusalem, Israel Signal transduction and propagation at the nociceptive peripheral terminals 6. Omer Barkai, The Hebrew University of Jerusalem, Israel A new mechanism of opioid-induced tolerance and hyperaglesia 7. Winnie Wefelmeyer, King's College London, UK Activity-dependent mismatch between axo-axonic synapses and the axon initial segment controls neuronal output 13