TRANSCRIPTION CAPPING
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Transcription
1 Messenger RNA
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3 Eucaryotic gene expression transcription START site exons upstream elements promoter elements TRANSCRIPTION CAPPING introns gene m 7 G pre-mrna m 7 G SPLICING POLYADENYLATION AAAAAAAAAn polyadenylated pre-mrna m 7 G TRANSPORT AAAAAAAAAn mature mrna TRANSLATION
4 mrna structures AUG UAA cap AAAAAAAAAAAAA 5 UTR 3 UTR ORF
5 Eucaryotic gene expression transcription START site exons upstream elements promoter elements TRANSCRIPTION CAPPING introns gene m 7 G pre-mrna m 7 G SPLICING POLYADENYLATION AAAAAAAAAn polyadenylated pre-mrna m 7 G TRANSPORT AAAAAAAAAn mature mrna TRANSLATION
6 Why the Cap structure is important? 1) RNA stability 2) Favours the mrna transport to the cytoplasm 3) Increases transla?on (it binds to eif4e that belongs to transla?on ini?a?on complex)
7 7-metilguanosina This involves the addi?on of a modified guanine base to the 50 end of the RNA. Specifically, it is a methylated guanine, and it is joined to the RNA transcript by an unusual 5 5 linkage involving three phosphates CAP is added at very early stage of transcrip?on ini?a?on estremità 5 della RNA catena The 5-5 phosphodiester bond makes the molecule resistant to the exonuclease ac?vity. I vitro synthe?zed RNA without CAP are rapidly degraded
8 pre-mrna capping The 5 cap is created in three enzyma?c steps: 1. a phosphate group is removed from the 5 end of the transcript. RNA triphosphatase 2. GMP moiety is added. RNA guanylyltransferase 3. GMP nucleo?de is modified by the addi?on of a methyl group. RNA(G-7-) methyltransferase
9 5 CAP favours the mrna transport to the cytoplasm CBC: CAP binding complex
10 Eucaryotic gene expression transcription START site exons upstream elements promoter elements TRANSCRIPTION CAPPING introns gene m 7 G pre-mrna m 7 G SPLICING POLYADENYLATION AAAAAAAAAn polyadenylated pre-mrna m 7 G TRANSPORT AAAAAAAAAn mature mrna TRANSLATION
11 How is the func4on of the polya tail? 1) RNA stability 2) Favours the mrna transport to the cytoplasm 3) Increases transla?on efficiency by favou?ng the loading of ribosomal 40S subunit 4) mrna 3 end forma?on allows efficient transcrip?on termina?on.
12 Looking for consenus sequence SEQUENCE ALLINEAMENT OF cdnas STARTING FROM THE POLYA TAIL 5 UTR 3 UTR nt globin AUG UAA GGUUAUCCAUCAAUAAA.GCUAUACGCAAAA n Ig AUG UAA CCACUGGGCCAAUAAA.GCUAUACGCAAAA n Ovoalbumin AUG UAA GCAACCUCGAAUAAA.GCUAUACGCAAAA n Polymerase AUG UAA AUCUGGAGGAAUAAA.GCUAUACGCAAAA n Myc AUG UAA UAUAGAUCCAAUAAA.GCUAUACGCAAAA n AAUAAA consensus
13 Efficiency consensus Polyadenyla?on efficiency Influence of consensus sequence AAUAAA!
14 Site- specific muta4on Recombinant DNA preparation * mutazione transfection mut1 mut2 mut3 WT polya Northern blot RNA extraction After 24 h HeLa cells
15 3 end forma?on in mammalian cells Cleavage and polyadenyla?on site 5 AAUAAA GU rich nt 5 AAUAAA OH P GU rich 3 5 AAUAAA A 250 degrada?on
16 Cleavage and Polyadenylation Specific Factor" CPSF! 5 AAUAAA CA SPECIFIC COMPLEXES ARE INVOLVED IN mrna 3 END PROCESSING sito di taglio Cleavage Stimulation Factor" CstF! " PAP 3 Poly- A polymerase GU- rich CFII! CFI! Curr Opin Cell Biol Jun;16(3):272-8" HeLa NE AAUAAA AAGAAA complex EMSA assay for tes?ng the interac?on between CPSF 160 and consensus sequence AAUAAA sito di taglio free RNA
17 3 - End Forma4on: RNA Processing Cleavage and Polyadenyla?on Specific Factor Cleavage Factors Cleavage S?mula?on Factor
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20 degrada4on 20
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23 CAP and polya tail influence efficient translation
24 Polyadenylation is linked to termination mrna Torpedo Model AAAAAAAAA pre- mrna CPSF pa Xrn2 CstF RNAPII RNAPII
25 Alterna?ve polyadenyla?on of the immunoglobulin μ heavy chain gene sub obtimal pa obtimal pa Secreted terminus Membrane bound terminus Secreted terminus Membrane bound terminus
26 CSTF- 64 levels control the alterna?ve processing of mrna. In not ac?vated B cells the limi?ng concentra?on of CSTF allows the recogni?on of the stronger polyadenyla?on signal. The immuglobulin produced will then contain a por?on for binding to the membrane. Aler the ac?va?on of B cells CSTF levels are increased and this allows the use of the weaker polyadenyla?on site that will be preferen?ally used because it will be the first to be transcribed. CstF-64 The polyadenylation factor CstF-64 regulates alternative processing of IgM heavy chain premrna during B cell differentiation. Takagaki Y, Seipelt RL, Peterson ML, Manley JL. Cell 1996 Nov 29;87(5):941-52
27 Eucaryotic gene expression transcription START site exons upstream elements promoter elements TRANSCRIPTION CAPPING introns gene m 7 G pre-mrna m 7 G SPLICING POLYADENYLATION AAAAAAAAAn polyadenylated pre-mrna m 7 G TRANSPORT AAAAAAAAAn mature mrna TRANSLATION
28 Eukaryotic genes contain introns! Fig. 14.2
29 Identification of introns by R-looping Gene dell ovalbumina di pollo ibridato con il suo mrna e visualizzato al microscopio elemronico Exon:intron organization of the ovalbumin gene Exon Intron
30 Specific sequences inside the pre- mrna indicate where the spicing event has to take place. These sequences indicate the boundary between introns and exons.
31 Consensus Sequences Surrounding the 5 and 3 Splice Sites 31
32 The intron is removed in a Form Called Lariat and the Flanking Exons are joined Two trans- esterifica4ons: Step 1: The OH of the conserved A at the branch site amacks the phosphoryl group of the conserved G in the 5 splice site. As result, the 5 exon is released and the 5 - end of the intron forms a three- way junc?on structure. Step 2: The OH of the 5 exon amacks the phosphoryl group at the 3 splice site. As a consequence, the 5 and 3 exons are joined and the intron is released in a lariat shape
33 Nuclear pre-mrna splicing proceeds through two trans-esterification reactions! Fig. 14.4
34 Splicing occurs in a spliceosome an RNA-protein complex spliceosome (~100 proteins + 5 small RNAs) U1, U2, U4/U5/U6 pre- mrna spliced mrna Splicing works similarly in different organisms, for example in yeast, flies, worms, plants and animals.
35 Five snrnas are involved in pre-mrna splicing"
36 U4/U5/U6 tri-snrnps U1 and U2 snrnps
37 Ⅰ Assembly, rearrangement, and catalysis within the spliceosome: the splicing pathway Assembly step 1 1. U1 recognizes 5 splice site. 2. One subunit of U2AF binds to Py tract and the other to the 3 splice site. The former subunits interacts with BBP and helps it to bind to the branch point. 3. Early (E) complex is formed U1 BBP U2AF pg A 3 E complex
38 Assembly step 2 1. U2 binds to the branch site, and then A complex is formed. 2. The base- pairing between the U2 and the branch site is such that the branch site A is extruded. This A residue is available to react with the 5 splice site. U1 U2AF pg A 3 U2 A complex
39 Assembly step 3 1. U4, U5 and U6 form the tri- snrnp Par?cle. 2. With the entry of the tri- snrnp, the A complex is converted into the B complex. U1 U2AF pg A 3 U6 U5 U4 U2 U2AF65 35 A complex U5 U1 U2 U6 U4 A B complex
40 U1 U5 U2 B complex U6 U4 A U1 U6 U5 U4 U2 A A C complex in which the catalysis has not occurred yet U4 U6 U5 U2 A
41 Catalysis Step 1 Forma?on of the C complex produces the ac4ve site, with U2 and U6 RNAs being brought together. Forma?on of the ac?ve site juxtaposes the 5 splice site of the pre- mrna and the branch site, allowing the branched A residue to a]ack the 5 splice site to accomplish the first transesterfica?on reac?on. U4 U6 U5 U2 A
42 Catalysis Step 2 U5 snrnp helps to bring the two exons together, and aids the second transesterifica?on reac?on, in which the 3 - OH of the 5 exon amacks the 3 splice site. Final Step Release of the mrna product and the snrnps. U5 U5 U6 U2 A U6 U2 A
43 Spliceosome & ATP RNA- RNA Rearrangements - I 43
44 Spliceosome & ATP RNA- RNA Rearrangements - II 44
45 Pre- mrna Splicing is Accelerated by RNA- RNA Basepairing: snrnps 45
46 How to study splicing in vitro procedure -in vitro transcribed RNA -RNA plus nuclear extract -RNA isolation ot different time points -elettrophoresis for the analysis of the reaction products RNA analysis RNA isolation nuclear extract
47 Recognition of canonical splice sites?? BBP GU A AG GU YYYYYY 10-15% of single nucleo?de muta?ons causing disease affect splice sites From: H. Stark, P. Dube, R. Luhrmann & B. Kastner, Nature 409, (2001)
48 RNA splicing gets the most out of genes In animals, complexity depends less upon the number of genes than upon the number of different ways they can splice the RNA
49 Genome sequence analysis! Introns are abundant! 94% human, 85% fruitfly, 95% nemtode, 95% plant genes! Human genome (26,000-35,000 genes)! Only 2-5 times as many genes as in fruitflies (13,600)! or in nematodes (19,000)! Alternative splicing is abundant!! ~75% of human gene transcripts are alternatively spliced!
50 Alternative splicing The presence of mul?ple introns in many eukaryo?c genes permits expression of mul?ple, related proteins from a single gene by means of alterna?ve slicing, an important mechanism for the produc?on of different forms of proteins, called isoforms, by different types of cells. Nearly 75% of all human genes are expressed as alterna?vely spliced mrnas, leading to an expansion of the coding capacity of our genome. intron 1 intron 2 intron 3 intron Usually all introns must be removed before the mrna can be translated to produce protein
51 Alternative Splicing! 5 splice site switching! 3 splice site switching! Exon Skipping! Mutually exclusive exons! Intron retention!
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55 Splicing modulators
56 Multiple introns may be spliced differently in different circumstances, for example in different tissues. Heart muscle mrna pre- mrna Uterine muscle mrna hus one gene can encode more than one protein. The proteins are similar but not identical and may have distinct properties. This is important in complex organisms
57 Detec?on of alterna?ve splicing by Northern blorng Total RNA Transfer solution RNA Probe against exon
Messenger RNA maturation. Dr.ssa Mariangela Morlando
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Processing of RNA II Biochemistry 302. February 13, 2006
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