Habilitation à Diriger des Recherches (HDR) in Physiology, Université de Lille 1, Villeneuve d Ascq, France

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Abigayle Stevenson
5 years ago
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Present position and affiliation Associate Professor in Physiology Junior member of the Institut Universitaire de France Laboratory, INSERM U.

fr Diplomas 2013 Habilitation à Diriger des Recherches (HDR) in Physiology, Université de Lille 1, Villeneuve d Ascq, France 2006 PhD in Medical Sciences Radboud University Medical Centre Nijmegen,

1 Present position and affiliation Associate Professor in Physiology Junior member of the Institut Universitaire de France Laboratory, INSERM U.1003 Univeristé de Lille Sciences et Technologies Villeneuve d Ascq, France Tél Fax dimitra.gkika@univ-lille1.fr Diplomas 2013 Habilitation à Diriger des Recherches (HDR) in Physiology, Université de Lille 1, Villeneuve d Ascq, France 2006 PhD in Medical Sciences Radboud University Medical Centre Nijmegen, the Netherlands 2000 Master in Molecular and Cellular Endocrinology, Development specialization Faculty of Medicine, University Montpellier I, France 1999 Ptychion (diploma) in Biological Sciences Cell Biology specialization Faculty of Science, University of Athens, Greece Geographic and thematic mobility During my scientific career, I had the chance to explore different aspects of Life Sciences. I started in the Biophysics and Cancer Cell Biology field during the end of my basic training and continued on Molecular Genetics and Molecular Endocrinology during my Master Degree. I then worked as Research Associate in clinics and was interested in Stem Cell Biology and Immunity and changed in Cellular and Molecular Nephrology for my PhD. Later I was interested in Nociception and Thermosensation Physiology and currently I am working in Cancer Biology and Pathophysiology Associate Professor in Physiology, Role of TRP channels in migration of epithelial and endothelial cells during prostate cancer progression and angiogenesis, Cell Physiology Laboratory, INSERM U.1003, University of Lille 1, Villeneuve d Ascq, France Research Associate, Unraveling the molecular determinants of TRPM8 and TRPV2 channel regulation and its consequences in carcinogenesis, Cell Physiology Laboratory, INSERM U.1003, University of Lille 1, Villeneuve d Ascq, France

2 Postdoctoral fellow: Molecular insight into the role of TRPM8 plasma membrane localization during prostate cancer development and thermosensation Cell Physiology Laboratory, INSERM U.1003, University of Lille 1, Villeneuve d Ascq, France PhD trainee: Functional regulation of the epithelial calcium channel Department of Cell Physiology, Nijmegen Centre for Molecular Sciences, Radboud University Medical Centre Nijmegen, the Netherlands Assistant in research: Study of ontology, physiology and function of NK subpopulations Department of Immunology and Immunotherapy, St. Sawas Hospital, Athens, Greece 2000 Research trainee: Characterization of polymorphic markers specific for the pericentromeric region of the chromosome 21 Institute of Human Genetics of Montpellier, France 1999 Research trainee: Study of the effects of ionizing irradiation on pancreatic cells Department of Cell Biology, University of Athens, Greece Research activity TRP channels are considered as multiple signal integrators and as such they integrate external and endogenous stimuli maintaining many forms of homeostasis. The last 10 years, my research was based on unraveling both the extra and intracellular molecular factors regulating TRPs and its consequences in physiopathology including thermosensation, pain sensibility, carcinogenesesis and tumor angiogenesis. I am currently focusing on the functional interactions of TRPs with partner proteins, kinases, proteases, and hormones and describe the molecular mechanisms through which those factors influence channel activity, cell physiology and human physiopathology. My objective for the following years is to characterize the role of TRP channels in prostate tumor growth and vascularization on the molecular, cellular and whole organism level. With this project, I participate in the French network of excellence (LabEx) on the topic of channelopathies in order to identify TRPs and their modulators as novel drug targets for therapeutic and diagnostic use Current Research Project Prostate cancer (PCa) is the most common non-cutaneous human malignancy and PCa metastases are considered incurable. It is therefore critical to understand and target the molecular mechanisms by which tumor grow and spread. Being involved in nearly all of the hallmarks of cancer, TRP channels play a significant role in cancer progression and constitute potential novel therapeutic, diagnostic, and prognostic targets.

3 To date the data linking specific TRP channels to PCa cell migration, invasion and metastasis are still largely phenomenological while the molecular downstream components of these channels remain largely unknown. The main objectives of this project are to: 1) identify the TRP channel signature of PCa progression in clinical samples, 2) unravel and target the molecular mechanism through which the most promising TRP candidates act in cell migration, invasion and angiogenesis, 3) define in vivo the role of the selected channels in primary tumor development and metastasis development. Summary of professional achievements During my research activity, I developed a long-standing expertise on TRP channels and their role in physiopathology that was described in 28 publications (13 as first author, 4 as last and corresponding author in high impact international journals such as J Cell Biol, Cancer Cell, Cell Rep, J Clin Invest, EMBO J and Cancer Research) and presented upon invitation in prestigious international meetings such as Gordon Conferences, SfN and European Physiology Societies meetings. I recently initiated a group with a novel research axis on TRP involvement in cell migration and angiogenesis during carcinogenesis that was evaluated as one of the two most promising axes of the Inserm U1003 by the French Evaluation Agency for Research and Higher Education (AERES 2014). To support my research, I raised several grants such as ARC-2014 and ARTP-2015 and stipends for international PhD students (Vinci ). Following my HDR I tutored 2 PhD students. Since 2017 I was elected Junior member of the Institut Universitaire de France (IUF). Grants 2015 Grant of the ARC Foundation (Agency for Cancer Research) Amount: 50 k 2014 Grant of the ARTP Association (Research Association for Research on Prostate Tumors) Amount: 20 k 2013 IFR147 grant on «Emergence in proteomics and mass spectrometry» Amount: 4 k euros 2012 Vinci Project Université Franco-Italienne 3 years fellowship for a PhD student Amount: 60 k euros 2010 Bonus Quality Research (BQR) - Emerging Projects Amount: 30 k euros

4 Selected Publications For full publication list please click here TRPM8 inhibits endothelial cell migration via a non-channel function by trapping small GTPase, Rap1 Genova T*, Grolez GP*, Camillo C, Bernardini M, Bokhobza A, Richard E, Scianna M, Lemonnier L, Valdembri D, Munaron L, Philips MR, Mattot V, Serini G, Prevarskaya N*, Gkika D*, Fiorio Pla F* J Cell Biol in press Bernardini M, Fiorio Pla A, Prevarskaya N and Gkika D. Human transient receptor potential (TRP) channels expression profiling in carcinogenesis. Int. J. Dev. Biol : Gkika D*,#, Lemonnier L*, Shapovalov G*, Poux C, Bernardini M, Gordienko D, Bokhobza A, Gabriel Bidaux, Degerny C, Verreman K, Guarmit B, Benahmed M, de Launoit Y, Bindels R, Fioro Pla A, Prevarskaya N. TRP Channel-Associated Factors Are a Novel Protein Family That Regulates TRPM8 Trafficking and Activity. J Cell Biol Jan 5;208(1): doi: /jcb Fiorio Pla A*, Brossa A*, Bernardini M*, Genova T, Guilaume G, Villers A, Leroy X, Prevarskaya N, Bussolati B*, Gkika D*. Differential sensitivity of prostate tumor derived endothelial cells to sorafenib and sunitinib. BMC Cancer Dec 12;14:939. doi: / Gkika D*, Shapovalov G*, Devilliers M, Gordienko D, Kondratskyi A, Busserolles J, Bokhobza A, Eschalier A, Skryma R, Prevarskaya N. Opiates modulate thermosensation by internalizing cold receptor TRPM8. Cell Rep Aug 15;4(3): doi: /j.celrep Fioro Pla A and Gkika D. Emerging role of TRP channels in cell migration: from tumor vascularization to metastasis. Front. Physiol Nov 5;4:311. doi: /fphys Gkika D, Flourakis M, Lemonnier L, Prevarskaya N. PSA reduces prostate cancer cell motility by stimulating TRPM8 activity and plasma membrane expression. Oncogene Aug 12;29(32): doi: /onc Epub 2010 Jun 7. Gkika D, Topala CN, Chang Q, Picard N, Thébault S, Houillier P, Hoenderop JG, Bindels RJ. Tissue kallikrein stimulates Ca(2+) reabsorption via PKC-dependent plasma membrane accumulation of TRPV5. EMBO J Oct 18;25(20): Epub 2006 Sep 28. PubMed PMID: ; PubMed Central PMCID: PMC

5 Teaching activity My teaching service in the University of Lille the last five years fluctuates from 200 to 230 hours, mainly in Cell Physiology, Cell Biology, Main Physiological Animal Functions and Biotechnologies. Part of my teaching is given in English since I actively participated in setting up English of the bilingual teaching program in University of Lille since its beginning in My teaching hours are well equilibrated between lectures, theoretical and practical tutorials and comprise courses in Bachelor level as well as the two years of the Master, seminars for PhD students abroad. In addition, I regularly tutor students for their trainee during the last year of the bachelor (3 in the last five years) as well as the two years of the master (7 in the last five years, two of which were Erasmus students). Pedagogic duties Responsible for the course Main Animal Physiological Functions, 1st year, Bachelor 1st year, University of Lille 1 (since 2016) Responsible for the course Initiation in Biotechnologies, Bachelor 1st year, University of Lille 1 (since ) Participation in the bilingual (French / English) teaching program of Lille 1 University (since 2013) Reference Tutor for 1st year students (since 2011) Administrative duties Membre of the Scientific Committee the Association pour la Recherche sur les Tumeurs de la Prostate (ARTP) Reviewer for Medical Research Council Research Grant (since 2012) Reviewer for ARC (Agency for Research on Cancer) grants Member of the Editorial Board of the journal «Advances in Urology» BioMed Research International (since 2013) Reviewer for International Peer-Reviewed Journals such as Oncogene, Cancer Res, J Cell Biol, J Clin Oncology, Am J Pathol, BBA, JOCES, Cancers. Responsible (N+1) of one Inserm technician (Ingénieur d Etudes) Member of the Biology Department Counsil of Lille University (since 2015) Member of the selection committees, CNU section 66 of of Lille University (since 2013)

Tumour Biology. Programme course 7,5 credits Tumörbiologi 8MEA12 Valid from: 2018 Autumn semester. Determined by. Date determined.

Tumour Biology. Programme course 7,5 credits Tumörbiologi 8MEA12 Valid from: 2018 Autumn semester. Determined by. Date determined. DNR LIU-2014-01733 1(5) Tumour Biology Programme course 7,5 credits Tumörbiologi 8MEA12 Valid from: 2018 Autumn semester Determined by The Board for First and Second Cycle Programmes at the Faculty of