Gaining New Insights Through IF Multiplexed Staining and Analysis. Tyna Hope, Ph.D. P.Eng Biomarker Imaging Research Laboratory October 5, 2017
|
|
- Kelley Stokes
- 5 years ago
- Views:
Transcription
1 Gaining New Insights Through IF Multiplexed Staining and Analysis Tyna Hope, Ph.D. P.Eng Biomarker Imaging Research Laboratory
2 Assessing more from Tissue Sections
3 Gaps with More Common Methods Most common practice is to assess via individual IHC on sequential serial sections (different cells) Multispectral methods are limited in the number of markers, because of the need to separate the colour stains in the same pixel
4 MxIF HER/neu ER PgR Ki67 Cytokeratin DAPI ER + PgR + Ki67 + Cytokeratin HER/neu + ER + PgR 4
5 Sequential Stain and Bleach Acquire background AF Image Cy-Ab Cy5-Ab Stain slide with Ab and Ab Bleach Fluorophor >60 proteins Acquire IF image 5
6 Multiplexing Method Formalin fixed and paraffin embedded tissue samples. Automatic sequential stain-imagebleach approach utilizing direct antibody-fluorophore conjugation. Multiplexing with GE s MxIF We are also able to stain and bleach manually by use the imaging equipment. 6
7 Application in Ovarian Cancer Research CD + CD4 CD4 + CD8 CD + CD4 + CD8 CK + CD4 + CD8 CD Red CD4 Green CD8 Blue Cytokeratin purple 7
8 Application in Breast Cancer Research ER PR HER Ki67 P CD4 CD8 8
9 Our First Experiment Breast cancer biomarker panel: ER, PR, Ki67, HER Segmentation markers: Dapi, cytokeratin, NaKATPase, S6 First generation equipment 9
10 Goals Determine if the signal correlated with the known grade of the patient Determine if the signal correlated with the pathologists scores Assess co-localization of biomarkers 0 October, 07
11 Calibration and Validation Data TMA Patients, cores Cores included Biomarkers AS000 55, 44 ER and PR AS000 50, 6 Ki67 and Her AS , 99 ER,PR,Ki67,HE R Tissue microarrays were intended to be used for calibration (AS000,AS000) and validation (AS4000) during model development. Omitted cores are due to missing tissue.
12 Technique Development Cy Cy5 We had to change the protocol to prevent false positive signals. Her ER Antibody species cross-reaction caused the nuclear staining ki67 to appear to stain the membrane. Bleached PR Ki67 Dan Wang, et al., AIMM 05 August 5 Wang D, Pang Z, Clarke GM, Nofech-Mozes S, Liu K, Cheung A, Filkins RJ,Yaffe MJ. Ki-67 membranous staining biologically relevant or an artifact of multiplexed immunofluorescent staining. Applies Immunohistochemistry & Molecular Morphology 05 Aug 5.
13 Technique Development ER PgR HER/neu Ki67 Cy5 Her ER ER PgR HER/neu Bleached Ki67 Bleached PR 00 um Ki67 Dan Wang, et al., AIMM 05 August 5 Validation of IF staining protocol using IHC stained serial sections.
14 Discussion Plan: Develop methods on calibration data and assess on validation data. Reality: We had limit our assessment to ER and HER in the calibration set. WHY? Cross-reactivity meant PR and Ki67 signals were questionable for calibration data set Validation signal magnitudes were much different than the calibration set 4
15 Results Models were developed on ER and HER from the calibration data set only. We found an association between the image signal and pathologists IHC score. Complex models, using groups of decision trees Scale and intensity invariant features Used out-of-bag (OOB) error rate (like cross-validation) ER model: OOB 5.7% (94.% accuracy), 95% CI (0.89, 0.98). HER model: OOB 4.6% (95.4 % accuracy) 5
16 Companion Software Visualize as H&E Obtain quantitative information on each cell. 6 Nuclear, cytoplasm, membrane segmentation
17 In-house Developed tools ER PgR Ki
18 In-house Developed Tools 8
19 9 In-house Developed Tools Tumour A microenvironment Tumour A Tumour B microenvironment Tumour B
20 Quantification of relationships Our goal is to describe the spatial arrangement of types of cells making up the tumour and/or the microenvironment. This may help us understand the heterogeneity of cell populations within a patient and across patients. 0
21 Finishing Touches
22 Biomarker Imaging Research Laboratory Lab Members Martin Yaffe, Ph.D., C.M. Principle Investigator Gordon Mawdsley, BSc, FCCPM, P.Phys. Lab Manager Alison Cheung, PhD Research Associate Yulia Yerofeyeva, M.Sc. Program Manager Kela Lui, MD Immunohistochemistry Technologist Dan Wang. M.Sc. Immunohistochemistry Technologist Taha Rashed BHc (MLS), MLT Lead Medical Laboratory Technologist Rachel Peters, AIMLS (UK), FIMLS (UK), ART (Can) Medical Laboratory Technologist Adebayo Adeeko, FIScT, PG Dip., MLA/T Medical Laboratory Technician Mayan Murray, M.Eng., EIT Research Engineer Reach us:
Immune Cell Phenotyping in Solid Tumors using Quantitative Pathology
Immune Cell Phenotyping in Solid Tumors using Quantitative Pathology James R. Mansfield Director of Quantitative Pathology Applications 2009 PerkinElmer What is Quantitative Pathology? Quantitative Pathology
More informationAbstract. Background. Objective
Molecular epidemiology of clinical tissues with multi-parameter IHC Poster 237 J Ruan 1, T Hope 1, J Rheinhardt 2, D Wang 2, R Levenson 1, T Nielsen 3, H Gardner 2, C Hoyt 1 1 CRi, Woburn, Massachusetts,
More informationLayered-IHC (L-IHC): A novel and robust approach to multiplexed immunohistochemistry So many markers and so little tissue
Page 1 The need for multiplex detection of tissue biomarkers. There is a constant and growing demand for increased biomarker analysis in human tissue specimens. Analysis of tissue biomarkers is key to
More informationNext-Gen Analytics in Digital Pathology
Next-Gen Analytics in Digital Pathology Cliff Hoyt, CTO Cambridge Research & Instrumentation April 29, 2010 Seeing life in a new light 1 Digital Pathology Today Acquisition, storage, dissemination, remote
More informationSpatially resolved multiparametric single cell analysis. Technical Journal Club 19th September 2017 Christina Müller (Group Speck)
Spatially resolved multiparametric single cell analysis Technical Journal Club 19th September 2017 Christina Müller (Group Speck) Why spatially resolved multiparametric single cell analysis? Multiparametric
More informationQuality Assurance and Quality Control in the Pathology Dept.
Quality Assurance and Quality Control in the Pathology Dept. Judith Sandbank M.D. Pathology Assaf-Harofeh Medical Center ISRAEL jsandbank@asaf.health.gov.il 2 nd IBDC, 9 th February, 2012 Pathology as
More informationNext-Generation Immunohistochemistry: Multiplex tissue imaging with mass cytometry
Nat Met, April 2014 Nat Med, April 2014 Next-Generation Immunohistochemistry: Multiplex tissue imaging with mass cytometry Journal Club Timo Böge Overview Introduction Conventional Immunohistochemistry
More informationVisual interpretation in pathology
13 Visual interpretation in pathology Tissue architecture (alteration) evaluation e.g., for grading prostate cancer Immunohistochemistry (IHC) staining scoring e.g., HER2 in breast cancer (companion diagnostic
More informationBreast Cancer Diversity Various Disease Subtypes Clinical Diversity
Breast Cancer Predictive Factor Testing: The Challenge and Importance of Standardizing Pre- Analytic Variables David G. Hicks MD Professor of Pathology & Laboratory Medicine Director of Surgical Pathology
More informationNordiQC External Quality Assurance in Immunohistochemistry
NordiQC External Quality Assurance in Immunohistochemistry Mogens Vyberg Professor of Clinical Pathology Director of NordiQC Aalborg University Hospital, Aalborg, Denmark AALBORG (~ 200.000 inhabitants)
More informationCentral Pathology Review and Tissue MicroArrays. Dr Lisa Storer Children s Brain Tumour Research Centre Nottingham
Central Pathology Review and Tissue MicroArrays Dr Lisa Storer Children s Brain Tumour Research Centre Nottingham What is a tissue microarray? Tissue microarrays (TMAs) consist of paraffin blocks in which
More informationCC01 - Colon Cancer Tissue Microarray
Reveal Biosciences offers Histochemical Staining, Immunohistochemistry (IHC), In Situ Hybridization (ISH), Whole Slide Imaging, and Quantitative Image Analysis on any TMA CC01 - Colon Cancer Tissue Microarray
More informationLN04 - Lymphoma Tissue Microarray
Reveal Biosciences offers Histochemical Staining, Immunohistochemistry (IHC), In Situ Hybridization (ISH), Whole Slide Imaging, and Quantitative Image Analysis on any TMA LN04 - Lymphoma Tissue Microarray
More informationFAQs for UK Pathology Departments
FAQs for UK Pathology Departments This is an educational piece written for Healthcare Professionals FAQs for UK Pathology Departments If you would like to discuss any of the listed FAQs further, or have
More informationImage analysis in IHC overview, considerations and applications
Image analysis in IHC overview, considerations and applications Rasmus Røge, MD, Institute of Pathology, Aalborg University Hospital NordiQC workshop September 2016 Aalborg, Denmark Outline Theory Image
More informationOptimal algorithm for HER2 testing
Optimal algorithm for HER2 testing The revised definition of IHC 2+ (equivocal) is invasive breast cancer with Weak to moderate complete membrane staining observed in >10% of tumor cells. (see Figure 1
More informationBreast cancer: Antibody selection, protocol optimzation controls and EQA
Breast cancer: Antibody selection, protocol optimzation controls and EQA Workshop in Diagnostic Immunohistochemistry Oud St. Jan/ Old St. John Brugge (Bruges), Belgium June 13th 15nd 2018 Rasmus Røge,
More informationUse of tissue micro array (TMA) in routine clinical analysis
2nd European Workshop on Tissue Imaging and Analysis June 25-26, 2010 Use of tissue micro array (TMA) in routine clinical analysis Tim Svenstrup Poulsen Molecular unit Dept. pathology Herlev Hospital Denmark
More informationQuality in Control. ROS1 Analyte Control. Product Codes: HCL022, HCL023 and HCL024
Quality in Control ROS1 Analyte Control Product Codes: HCL022, HCL023 and HCL024 Contents What is ROS1? 2 The Role of ROS1 in Cancer 3 ROS1 Assessment 3 ROS1 Analyte Control Product Details 4 ROS1 Analyte
More informationDeciphering the biology that drives response to immunotherapy
Deciphering the biology that drives response to immunotherapy Phenoptics TM Quantitative Pathology Platform Trent Norris, Field Application Scientist September 15, 2016 HUMAN HEALTH ENVIRONMENTAL HEALTH
More informationMT09 - Normal Human Tissue Microarray, FDA
Reveal Biosciences offers Histochemical Staining, Immunohistochemistry (IHC), In Situ Hybridization (ISH), Whole Slide Imaging, and Quantitative Image Analysis on any TMA MT09 - Normal Human Tissue Microarray,
More informationDigital Pathology and CAP Guidelines
Digital Pathology and CAP Guidelines Frequently asked questions The VENTANA family of digital pathology products empowers you with the convenience of a comprehensive image and workflow solution. When used
More informationApplying Tissue Phenomics to Colorectal Clinical Questions
Applying Tissue Phenomics to Colorectal Clinical Questions International Symposium for Tissue Phenomics San Francisco October 2014 Peter Caie Senior Research Fellow University of St Andrews Systems Pathology
More informationANALYTISCHE STRATEGIE Tissue Imaging. Bernd Bodenmiller Institute of Molecular Life Sciences University of Zurich
ANALYTISCHE STRATEGIE Tissue Imaging Bernd Bodenmiller Institute of Molecular Life Sciences University of Zurich Quantitative Breast single cancer cell analysis Switzerland Brain Breast Lung Colon-rectum
More informationProduct Introduction
Product Introduction Product Codes: HCL026, HCL027 and HCL028 Contents Introduction to HER2 2 HER2 immunohistochemistry 3 Cell lines as controls 5 HER2 Analyte Control DR IHC 7 HER2 Analyte Control DR
More informationRNA preparation from extracted paraffin cores:
Supplementary methods, Nielsen et al., A comparison of PAM50 intrinsic subtyping with immunohistochemistry and clinical prognostic factors in tamoxifen-treated estrogen receptor positive breast cancer.
More informationClinical Utility of Diagnostic Tests
Clinical Utility of Diagnostic Tests David A. Eberhard MD, PhD Director, Pre-Clinical Genomic Pathology, Lineberger Comprehensive Cancer Center Associate Professor, Depts. of Pathology and Pharmacology
More informationQuality Control/Quality Assurance in Diagnostic Immunohistochemistry
CIHRT Exhibit P- Page Quality Control/Quality Assurance in Diagnostic Immunohistochemistry Emina Torlakovic, MD, PhD College of Medicine University of Saskatchewan Emina Emilia Torlakovic, MD, PhD Associate
More informationACCME/Disclosures. Diagnosing Mesothelioma in Limited Tissue Samples. Papanicolaou Society of Cytopathology Companion Meeting March 12 th, 2016
Diagnosing Mesothelioma in Limited Tissue Samples Papanicolaou Society of Cytopathology Companion Meeting March 12 th, 2016 Sanja Dacic, MD, PhD University of Pittsburgh ACCME/Disclosures GENERAL RULES
More informationIncorporating pharmacodynamic, response and patient selection biomarkers. Paul Elvin PhD Chief Translational Science Officer Aptus Clinical
Incorporating pharmacodynamic, response and patient selection biomarkers Paul Elvin PhD Chief Translational Science Officer Aptus Clinical 22 Oncology drug development Biomarkers key for: Strong hypothesis
More informationUtility of Adequate Core Biopsy Samples from Ultrasound Biopsies Needed for Today s Breast Pathology
Utility of Adequate Core Biopsy Samples from Ultrasound Biopsies Needed for Today s Breast Pathology Ugur Ozerdem, M.D. 1 Abstract Background: There is a paradigm shift in breast biopsy philosophy. In
More informationBREAST CANCER NOW RESEARCH CENTRE ICR CHELSEA, LONDON. Higher Scientific Officer
BRAST CANCR NOW RSARCH CNTR ICR CHLSA, LONON Higher Scientific Officer The Institute of Cancer Research, London, is one of the world s most influential cancer research institutes, with an outstanding record
More informationSingle and Multiplex Immunohistochemistry
Single and Multiplex Immunohistochemistry Steve Westra, BS Reagent Product Specialist Leica Biosystems IHC Theory Polyclonal vs Monoclonal Polyclonal reagents Detect a multitude of epitopes Batch to batch
More informationMultiplex immunofluorescence for investigating the prognostic value of immune cells Chidozie Anyaegbu, PhD
Multiplex immunofluorescence for investigating the prognostic value of immune cells Chidozie Anyaegbu, PhD Catholic Health Australia Symposium Mater Hospital, Brisbane 1 st June 2018 Why colorectal cancer?
More informationAdequate Core Biopsy Samples from Stereotactic Biopsies Needed for Today s Breast Pathology
Adequate Core Biopsy Samples from Stereotactic Biopsies Needed for Today s Breast Pathology Ugur Ozerdem, M.D. 1 Abstract Background: There is a paradigm shift in breast biopsy philosophy. In the past
More informationPresent Role of Immunohistochemistry in the. Subtypes. Beppe Viale European Institute of Oncology University of Milan Milan-Italy
Present Role of Immunohistochemistry in the Classification of Molecular Subtypes Beppe Viale European Institute of Oncology University of Milan Milan-Italy We know it is many diseases Breast cancer is
More informationQuality assurance and quality control in pathology in breast disease centers
Quality assurance and quality control in pathology in breast disease centers Judith Sandbank M.D. Pathology Assaf-Harofeh Medical Center ISRAEL jsandbank@asaf.health.gov.il 1 st IBDC, 28 th January, 2011
More informationAssessment performed on Friday, September 18, 2015, at Vancouver General Hospital
Assessors report for ciqc Run 49: ATRX (June 2015) Assessors: S Yip and J Won (recorder) Assessment performed on Friday, September 18, 2015, at Vancouver General Hospital Background The combined application
More informationVENTANA MMR IHC Panel Interpretation Guide for Staining of Colorectal Tissue
VENTANA MMR IHC Panel Interpretation Guide for Staining of Colorectal Tissue VENTANA anti-mlh1 (M1) Mouse Monoclonal Primary Antibody VENTANA anti-pms2 (A16-4) Mouse Monoclonal Primary Antibody VENTANA
More informationHER2 CISH pharmdx TM Kit Interpretation Guide Breast Cancer
P A T H O L O G Y HER2 CISH pharmdx TM Kit Interpretation Guide Breast Cancer FROM CERTAINTY COMES TRUST For in vitro diagnostic use HER2 CISH pharmdx Kit HER2 CISH pharmdx Kit is intended for dual-color
More informationSupplementary Online Content
Supplementary Online Content Bell EH, Pugh SL, McElroy JP, et al. Molecular-based recursive partitioning analysis model for glioblastoma in the temozolomide era: a correlative analysis based on NRG Oncology
More informationFISH mcgh Karyotyping ISH RT-PCR. Expression arrays RNA. Tissue microarrays Protein arrays MS. Protein IHC
Classification of Breast Cancer in the Molecular Era Susan J. Done University Health Network, Toronto Why classify? Prognosis Prediction of response to therapy Pathogenesis Invasive breast cancer can have
More informationCorporate Medical Policy
Corporate Medical Policy Microarray-based Gene Expression Testing for Cancers of Unknown File Name: Origination: Last CAP Review: Next CAP Review: Last Review: microarray-based_gene_expression_testing_for_cancers_of_unknown_primary
More informationGeisinger Clinic Annual Progress Report: 2011 Nonformula Grant
Geisinger Clinic Annual Progress Report: 2011 Nonformula Grant Reporting Period July 1, 2012 June 30, 2013 Nonformula Grant Overview The Geisinger Clinic received $1,000,000 in nonformula funds for the
More informationClaudin-4 Expression in Triple Negative Breast Cancer: Correlation with Androgen Receptors and Ki-67 Expression
Claudin-4 Expression in Triple Negative Breast Cancer: Correlation with Androgen Receptors and Ki-67 Expression Mona A. Abd-Elazeem, Marwa A. Abd- Elazeem Pathology department, Faculty of Medicine, Tanta
More informationReporting of Breast Cancer Do s and Don ts
Reporting of Breast Cancer Do s and Don ts 7 th SGH Annual Breast Pathology Course Professor Michael Bilous Conjoint Professor Western Sydney University Consultant Pathologist, Australian Clinical Labs,
More informationSupplementary materials and methods.
Supplementary materials and methods. Microarray printing and data analysis. The LNA-modified oligonucleotide probe set for all annotated mirnas from mouse (Mus musculus) and human (Homo sapiens) in the
More informationKi67 Index, HER2 Status, and Prognosis of Patients With Luminal B Breast Cancer
ARTICLE Ki67 Index, HER2 Status, and Prognosis of Patients With Breast Cancer Maggie C. U. Cheang, Stephen K. Chia, David Voduc, Dongxia Gao, Samuel Leung, Jacqueline Snider, Mark Watson, Sherri Davies,
More informationNSABP B-55/BIG Lynne Suhayda, RN, MSEd. Director, Clinical Coordinating Department. NRG Oncology - Pittsburgh, Pennsylvania
NSABP B-55/BIG 6-13 Lynne Suhayda, RN, MSEd. Director, Clinical Coordinating Department NRG Oncology - Pittsburgh, Pennsylvania NRG Oncology Meeting July 15, 2016 Lynne Suhayda, RN, MSEd. No Financial
More informationWelcome! HER2 TESTING DIAGNOSTIC ACCURACY 4/11/2016
HER2 TESTING DIAGNOSTIC ACCURACY Can t We Finally Get It Right? Allen M. Gown, M.D. Medical Director and Chief Pathologist PhenoPath Laboratories Seattle, Washington Clinical Professor of Pathology University
More informationApplications of IHC. Determination of the primary site in metastatic tumors of unknown origin
Applications of IHC Determination of the primary site in metastatic tumors of unknown origin Classification of tumors that appear 'undifferentiated' by standard light microscopy Precise classification
More informationSurgical Pathology Lab of the Future. Thomas M. Grogan, M.D. Professor of Pathology, University of Arizona Founder, Ventana Medical Systems, Inc.
Surgical Pathology Lab of the Future Thomas M. Grogan, M.D. Professor of Pathology, University of Arizona Founder, Ventana Medical Systems, Inc. 28 April 2010 Objective Demonstrate how the next generation
More informationLOCAL INFLAMMATION IN BREAST TISSUE AND MAMMOGRAPHIC DENSITY AMONG PREMENOPAUSAL AND POSTMENOPAUSAL WOMEN
LOCAL INFLAMMATION IN BREAST TISSUE AND MAMMOGRAPHIC DENSITY AMONG PREMENOPAUSAL AND POSTMENOPAUSAL WOMEN Mirette Hanna MD Clinical pathology PhD (candidate) Experimental medicine MSc Clinical and chemical
More informationProduct Introduction. Product Codes: HCL029, HCL030 and HCL031. Issue
Product Introduction Product Codes: HCL029, HCL030 and HCL031 Issue 1. 180510 Contents Introduction to Estrogen Receptor 2 ER immunohistochemistry 3 Quality control 5 Cell lines as controls 6 Estrogen
More informationFinal Project Report Sean Fischer CS229 Introduction
Introduction The field of pathology is concerned with identifying and understanding the biological causes and effects of disease through the study of morphological, cellular, and molecular features in
More informationPROTOCOL SENTINEL NODE BIOPSY (NON OPERATIVE) BREAST CANCER - PATHOLOGY ASSESSMENT
PROTOCOL SENTINEL NODE BIOPSY (NON OPERATIVE) BREAST CANCER - PATHOLOGY ASSESSMENT Author: Dr Sally Ann Hales On behalf of the Breast and pathology CNGs Written: March 2005 Reviewed by CNG: June 2009 &
More informationMesothelioma: diagnostic challenges from a pathological perspective. Naseema Vorajee August 2016
Mesothelioma: diagnostic challenges from a pathological perspective Naseema Vorajee August 2016 Naseema.vorajee@nhls.ac.za Pleural diseases (whether neoplastic, reactive or infective) may have similar
More informationTranslating Tissue Phenomics and Big Data Distillation to Clinical Histopathology
International Symposium for Tissue Phenomics 2016 Translating Tissue Phenomics and Big Data Distillation to Clinical Histopathology Dr Peter Caie Senior Research Fellow QUAntitative and Digital (QUAD)
More informationImportance of Methodology Certification and Accreditations to Perform Assays. Stan Hamilton, MD Head, Pathology and Laboratory Medicine
Importance of Methodology Certification and Accreditations to Perform Assays Stan Hamilton, MD Head, Pathology and Laboratory Medicine 1 Disclosures No disclosures relevant to this presentation 2 A bad
More informationAspects of quality in breast pathology. Andrew Lee Nottingham University Hospitals
Aspects of quality in breast pathology Andrew Lee Nottingham University Hospitals British breast pathology EQA: performance issues Ian Ellis Friday 8.30 am National breast screening pathology audit 2015
More informationSupplemental figure 1. PDGFRα is expressed dominantly by stromal cells surrounding mammary ducts and alveoli. A) IHC staining of PDGFRα in
Supplemental figure 1. PDGFRα is expressed dominantly by stromal cells surrounding mammary ducts and alveoli. A) IHC staining of PDGFRα in nulliparous (left panel) and InvD6 mouse mammary glands (right
More informationMolecular in vitro diagnostic test for the quantitative detection of the mrna expression of ERBB2, ESR1, PGR and MKI67 in breast cancer tissue.
Innovation for your breast cancer diagnostics Now valid ated on: -Roche cob as z 480 A nalyzer -Roche L ightcycle r 480 II - ABI 750 0 Fast -Versant kpcr Cyc ler PGR ESR1 MKI67 Molecular in vitro diagnostic
More informationMass Histology Service
Mass Histology Service A complete anatomical pathology laboratory www.masshistology.com Telephone: (877) 286-6004 Report on Pathology A Time Course Study of the Local Effects of Intramuscular XXXXXXX Injection
More informationUntangling the tumor microenvironment
Webinar Series Untangling the tumor microenvironment Illuminating the complex interactions & functions of immune cells December 10, 2014 Instructions for Viewers To share webinar via social media: To share
More informationAssessment of Risk Recurrence: Adjuvant Online, OncotypeDx & Mammaprint
Assessment of Risk Recurrence: Adjuvant Online, OncotypeDx & Mammaprint William J. Gradishar, MD Professor of Medicine Robert H. Lurie Comprehensive Cancer Center of Northwestern University Classical
More informationACCURACY OF IMMUNOHISTOCHEMISTRY IN EVALUATION
POL J PATHOL 2011; 2: 95-100 ACCURACY OF IMMUNOHISTOCHEMISTRY IN EVALUATION OF MALIGNANT PLEURAL AND PERITONEAL EFFUSIONS FERESHTEH ENSANI, FARNAZ NEMATIZADEH, GITI IRVANLOU Department of Cytology, Cancer
More informationInterpreting Therapeutic Response on Immune Cell Number and Spatial Distribution within the Tumor Microenvironment. Lorcan Sherry, CSO OracleBio
Interpreting Therapeutic Response on Immune Cell Number and Spatial Distribution within the Tumor Microenvironment Lorcan Sherry, CSO OracleBio Company Overview OracleBio is a specialised CRO providing
More informationClassification of the unknown primary tumour: the primary IHC panel
CIQC/CAP-ACP SEMINAR 2013: DIAGNOSTIC IHC AND MOLECULAR PATHOLOGY Classification of the unknown primary tumour: the primary IHC panel Aalborg University Hospital Denmark Tumours of unknown origin: Histology
More informationThe following slides were presented at the TIGA Workshop, and are enclosed in the PP show format (*.pps) in order to include all presented details.
Disclaimer: The following slides were presented at the TIGA Workshop, and are enclosed in the PP show format (*.pps) in order to include all presented details. These slides are proprietary and should not
More informationTransform genomic data into real-life results
CLINICAL SUMMARY Transform genomic data into real-life results Biomarker testing and targeted therapies can drive improved outcomes in clinical practice New FDA-Approved Broad Companion Diagnostic for
More informationPractical Applications of Digital Pathology
Digital pathology will continue to advance and provide precise prognostic information and help guide treatment decisions. Life in Stone. Photograph courtesy of Craig Damlo. www.soapboxrocket.com. Practical
More informationAdjuvan Chemotherapy in Breast Cancer
Adjuvan Chemotherapy in Breast Cancer Prof Dr Adnan Aydıner Istanbul University, Oncology Institute aa1 Slide 1 aa1 adnan aydiner; 17.02.2008 15-Year Reductions in Recurrence and Disease-Specific Mortality
More informationDifferential diagnosis of HCC
Hepatocellular Carcinoma Quest for an Ideal Immunohistochemical Panel Sanjay Kakar, MD UCSF Differential diagnosis of HCC Hepatocellular lesions Adenoma, FNH, HG dysplasia Adenocarcinoma CholangioCA, metastasis
More informationValidation of Novel Breast Cancer Biomarkers Arising from Re-Analysis of Publicly Available DNA Microarray Datasets via Tissue Microarray Technology
EORTC-NCI-ASCO Meeting on Molecular Markers in Cancer 15-17th November 2007, Brussels Validation of Novel Breast Cancer Biomarkers Arising from Re-Analysis of Publicly Available DNA Microarray Datasets
More informationResearch Article Ductal Breast Carcinoma In Situ: Mammographic Features and Its Relation to Prognosis and Tumour Biology in a Population Based Cohort
Hindawi International Journal of Breast Cancer Volume 2017, Article ID 4351319, 9 pages https://doi.org/10.1155/2017/4351319 Research Article Ductal Breast Carcinoma In Situ: Mammographic Features and
More informationImmunohistochemical principles The technical test approach. Pre-analytical parametres
Immunohistochemical principles The technical test approach Pre-analytical parametres Søren Nielsen Global Pathology Manager Agilent Technologies (Former Scheme Manager, NordiQC) 2 IHC project coordinator
More information(A) PCR primers (arrows) designed to distinguish wild type (P1+P2), targeted (P1+P2) and excised (P1+P3)14-
1 Supplemental Figure Legends Figure S1. Mammary tumors of ErbB2 KI mice with 14-3-3σ ablation have elevated ErbB2 transcript levels and cell proliferation (A) PCR primers (arrows) designed to distinguish
More informationComparison of Triple Negative Breast Cancer between Asian and Western Data Sets
2010 IEEE International Conference on Bioinformatics and Biomedicine Workshops Comparison of Triple Negative Breast Cancer between Asian and Western Data Sets Lee H. Chen Bioinformatics and Biostatistics
More informationCOMPUTER-AIDED HER-2/neu EVALUATION IN EXTERNAL QUALITY ASSURANCE (EQA) OF BREAST CANCER SCREENING PROGRAMME
COMPUTER-AIDED HER-2/neu EVALUATION IN EXTERNAL QUALITY ASSURANCE (EQA) OF BREAST CANCER SCREENING PROGRAMME Maria Lunardi MD Anatomic Pathology Fracastoro Hospital San Bonifacio, Verona -Italy HER2-neu
More informationHIGH CONTENT ANALYSIS WITH CELLULAR AND TISSUE SYSTEMS BIOLOGY: A BRIDGE BETWEEN CANCER CELL BIOLOGY AND TISSUE-BASED DIAGNOSTICS
Chapter for "The Molecular Basis of Cancer", 4th Edition (In Press) HIGH CONTENT ANALYSIS WITH CELLULAR AND TISSUE SYSTEMS BIOLOGY: A BRIDGE BETWEEN CANCER CELL BIOLOGY AND TISSUE-BASED DIAGNOSTICS Albert
More informationDr. dr. Primariadewi R, SpPA(K)
Curriculum Vitae Dr. dr. Primariadewi R, SpPA(K) Education : Medical Doctor from UKRIDA Doctoral Degree from Faculty of Medicine University of Indonesia Pathologist Specialist and Consultant from Faculty
More informationQuantitative Image Analysis of HER2 Immunohistochemistry for Breast Cancer
Quantitative Image Analysis of HER2 Immunohistochemistry for Breast Cancer Guideline from the College of American Pathologists Early Online Release Publication: Archives of Pathology & Laboratory Medicine
More informationAnti-DC-SIGN/CD209 murine monoclonal antibodies
Anti-DC-SIGN/CD209 murine monoclonal antibodies DC-SIGN (DC Specific, ICAM-3 Grabbing, Nonintegrin) / CD209 and L-SIGN (liver/lymph node-specific ICAM-3-grabbing nonintegrin CD299/ DC-SIGNR (DC-SIGN-related
More informationCompara've Medicine Animal Histology Services. Glossary of Terms
Compara've Medicine Animal Histology Services Glossary of Terms About this guide This guide is intended to provide clarifica'on on standard histologic terminology. Included are examples of different sample
More information# Best Practices for IHC Detection and Interpretation of ER, PR, and HER2 Protein Overexpression in Breast Cancer
#1034 - Best Practices for IHC Detection and Interpretation of ER, PR, and HER2 Protein Overexpression in Breast Cancer Richard W. Cartun, MS, PhD Andrew Ricci, Jr, MD Department of Pathology Hartford
More informationAnti-hTERT Antibody (SCD-A7)
Quality in Control Anti-hTERT Antibody (SCD-A7) htert_ab_pi_v1 Product Code: HCL025 Contents htert and Telomerase 2 Negative htert 3 Positive htert 4 Guidance and additional data 5 Case Study: RMH12-001
More informationWelcome. Nanostring Immuno-Oncology Summit. September 21st, FOR RESEARCH USE ONLY. Not for use in diagnostic procedures.
Welcome Nanostring Immuno-Oncology Summit September 21st, 2017 1 FOR RESEARCH USE ONLY. Not for use in diagnostic procedures. FOR RESEARCH USE ONLY. Not for use in diagnostic procedures. Agenda 4:00-4:30
More informationComparative Analysis of Methods Used in Breast Cancer HER2 and Sentinel Lymph Node Diagnosis
SHORT THESIS FOR THE DEGREE OF DOCTOR OF PHILOSOPHY (Ph.D.) Comparative Analysis of Methods Used in Breast Cancer HER2 and Sentinel Lymph Node Diagnosis by Monika Francz MD Supervisor: Zoltán Szöllősi
More informationGOALS AND OBJECTIVES BREAST PATHOLOGY
GOALS AND OBJECTIVES BREAST PATHOLOGY LEVEL: PGY2, PGY3, PGY5 A number of these rotations are introductory in nature, as they are major subspecialties, and are followed by two more blocks in PGY-3, during
More informationSupplementary figure 1. Supplementary Figure 1. Flowchart of experimental procedures of in situ hybridization using ViewRNA technology.
Supplementary figure 1 Supplementary Figure 1. Flowchart of experimental procedures of in situ hybridization using ViewRNA technology. Supplementary figure 2 Supplementary Figure 2. Evidence for existence
More informationImmunohistochemistry on Fluid Specimens: Technical Considerations
Immunohistochemistry on Fluid Specimens: Technical Considerations Blake Gilks Dept of Pathology University of British Columbia, Vancouver, BC, Canada Disclosures None Learning Objectives At the end of
More information* * * * Supplementary Figure 1. DS Lv CK HSA CK HSA. CK Col-3. CK Col-3. See overleaf for figure legend. Cancer cells
Supplementary Figure 1 Cancer cells Desmoplastic stroma Hepatocytes Pre-existing sinusoidal blood vessel New blood vessel a Normal liver b Desmoplastic HGP c Pushing HGP d Replacement HGP e f g h i DS
More informationDoes digital IHC need digital tissue controls?
VILNIUS UNIVERSITY Digital immunohistochemistry platform for the staining variation monitoring based on integration of image and statistical analyses with laboratory information system Arvydas Laurinavicius
More informationTemplate for Reporting Results of Biomarker Testing of Specimens From Patients With Carcinoma of the Breast
Template for Reporting Results of Biomarker Testing of Specimens From Patients With Carcinoma of the Breast Version: Template Posting Date: January 2018 Includes requirements from the 2017 CAP Accreditation
More informationJules Bordet Institute, Brussels, Belgium Université Libre de Bruxelles Breast International Group (BIG aisbl), Chair ESMO President
Symposium «Evaluation of the Belgian Cancer Plan» Brussels, November 26th, 2012 Personalized oncology in Europe: only a dream if national health systems do not get involved in diagnostics and pivotal cancer
More informationNHERF1 and tumor microenvironment: a new scene in invasive breast carcinoma
Saponaro et al. Journal of Experimental & Clinical Cancer Research (2018) 37:96 https://doi.org/10.1186/s13046-018-0766-7 RESEARCH NHERF1 and tumor microenvironment: a new scene in invasive breast carcinoma
More informationThe IO Quiz Show. The Complexity and Conundrums of the Tumor Microenvironment
The IO Quiz Show The Complexity and Conundrums of the Tumor Microenvironment Sacha Gnjatic, PhD Associate Professor, Department of Medicine Division of Hematology And Medical Oncology Tisch Cancer Institute
More informationDako IT S ABOUT TIME. Interpretation Guide. Agilent Pathology Solutions. ALK, ROS1 and RET IQFISH probes (Dako Omnis) MET IQFISH probe (Dako Omnis)
INTERPRETATION Dako Agilent Pathology Solutions IQFISH Interpretation Guide ALK, ROS1 and RET IQFISH probes (Dako Omnis) MET IQFISH probe (Dako Omnis) IT S ABOUT TIME For In Vitro Diagnostic Use ALK, ROS1,
More informationImmunohistochemistry in Bone and Soft Tissue Tumors. Sahar Rassi Zankoul, MD
Immunohistochemistry in Bone and Soft Tissue Tumors Sahar Rassi Zankoul, MD Introduction Bone tumors represent a wide variety of tumors of various origins and malignant potentials. These different tumor
More informationHSL-Advanced Diagnostics 2018 / 19 Test & Service List
HSL-Advanced Diagnostics 2018 / 19 Test & Service List 2018/19 TEST & SERVICE LIST Haematoxylin & Eosin H&E H&E per slide Routine Immunohistochemistry Immunohistochemical demonstration of an antigen in
More information