Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Spire Portsmouth Hospital Bartons Road Havant PO9 5NP United Kingdom Contact: Natalie Peck Website: Testing performed at the above address only DETAIL OF ACCREDITATION HUMAN BODY FLUIDS Haematology examination activities for the purpose of clinical diagnosis Blood Full Blood Count Documented in SOP SPS-HM- SOP0100 in conjunction with manufacturer s instructions for analysis using ABX Pentra 80 Red Blood Cells Mean Cell Volume Mean Cell Haemoglobin Mean Cell Haemoglobin Concentration Haematocrit Haemoglobin Platelets White Blood Cells Neutrophils Lymphocytes Eosinophils Monocytes Basophils Blood ESR Documented in SOP SPS-HM- SOP0600 using BD Seditainer TM modified Westergren method Blood Blood Film staining Documented in SOP SPS-HM- SOP0500 Manual staining using SpeedyDiff. Blood Sickle cell screening Microgen Bioproducts S-Test kit and SPS-HM-SOP800 Assessment Manager: MH1 Page 1 of 6

2 HUMAN BODY FLUIDS (cont d) Blood Haematology examination activities for the purpose of clinical diagnosis Coagulation screen PT, APTT, INR Blood Transfusion examination activities for the purpose of clinical diagnosis: Stago 4 analyser using viscosity based detection and SPS-HM- SOP0400 Blood ABO RhD Blood Grouping Documented in SOP SPS-BT- Using BioRad column agglutination Blood ABO RhD Blood Grouping Documented in SOP SPS-BT- Using manual tube techniques Plasma Antibody screening Documented in SOP SPS-BT- BioRad column agglutination Plasma Antibody screening Documented in SOP SPS-BT- Using manual tube techniques Blood Manual crossmatch Documented in SOP SPS-BT- Using Manual column agglutination in BioRad gel columns Assessment Manager: MH1 Page 2 of 6

3 HUMAN BODY FLUIDS (cont d) Biochemistry examination activities for the purpose of clinical diagnosis: General Biochemistry Quantification of: Urea and Electrolytes: Urea Creatinine Sodium Potassium Lactate Liver Function Test: Bilirubin Total protein (TP) Alanine Transaminase (ALT)) Alkaline Phosphatase (ALP) Aspartate Transaminase (AST) Gamma-Glutamyl Transpeptidase (GGT) Bone Profile: (TP, Albumin, ALP as above) Calcium Phosphate Lipid Profile: Cholesterol Triglyceride HDL Cholesterol Documented in SOP SPS-BC- SOP0100 in conjunction with manufacturer s instructions for analysis using Roche Cobas 311 Assessment Manager: MH1 Page 3 of 6

4 HUMAN BODY FLUIDS (cont d) Other assays: Amylase indices Glucose CRP Uric acid Magnesium Creatinine Kinase Calculated parameters egfr Globulin LDL cholesterol Adjusted calcium Urine βhcg Pregnancy testing using Alere hcg and SPS-GP-SOP1700 Arterial blood Blood gas: Documented in SOP SPS-GP- SOP1700 in conjunction with manufacturer s instructions for analysis using Osmetech Opti CCA Analyser ph po2 pco2 Bicarbonate Base Excess Oxygen Saturation Assessment Manager: MH1 Page 4 of 6

5 HUMAN BODY TISSUE Formalin Fixed Tissue Fresh tissue Fixed, fresh and frozen tissue Histopathological examination activities for the purposes of clinical diagnosis Preparation and examination of tissues to identify or exclude morphological abnormalities for the purpose of clinical diagnosis Basophilic and eosinophilic tissue structure Basophilic and eosinophilic tissue structures Macroscopic and microscopic examination using in-house procedures in conjunction with manufacturer s instructions for the following methods Macroanalysis and dissection using SOP SPS-HT-SOP-0200 Tissue Processing using Thermofisher Scientific Excelsior ES, Thermofisher Scientific Histostar. SOP s: SPS-HT-SOP0200, SPS-HT- SOP0300, SPS-HT-SOP0300, SPS-HT-SOP0400, SPS-HT- SOP0500 Frozen sections (intraoperative and Mohs) using Leica CM1950 Cryostat and H & E and toluidine blue staining using SPS-HT-PO-WI0503 Haematoxylin & Eosin (H&E) staining and HT-PO-WI0605 Assessment Manager: MH1 Page 5 of 6

6 HUMAN BODY TISSUE (cont d) Formalin fixed tissue Special stains for the detection of: Documented in house methods using stains as indicated Tissue mucins and fungi Acid mucins and neutral mucins Helicobacter pylori Haemosiderin and ferric iron Periodic Acid Schiff Alcian Blue/PAS Modified Giemsa Perls END Assessment Manager: MH1 Page 6 of 6

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