Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Cellular Pathology Department University College London Hospital Rockefeller Building University Street London WC1E 6JJ Contact: Gavyn

1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Cellular Pathology Department University College London Hospital Rockefeller Building University Street London WC1E 6JJ Contact: Gavyn Barrett Tel: +44 (0) Website: Testing performed at the above address only DETAIL OF ACCREDITATION FLUIDS Tissue: biopsy, excision, resection and post-mortem specimens diagnosis and cytological abnormalities for the purpose of diagnosis Specimen Dissection MP-CPTH-HIS-CUT UP LP-CPTH-HIS-POST MORTEM LP-CPTH-HIS-SPECIMEN OPENING EQUIPMENT LI-CPTH-HIS-MACROS Tissue processing LP-CPTH-HIS-PROCESSING 010-Linfo-CPTH-HIS-TISSUE TEK MANUAL 011a-Linfo-CPTH-HIS-TISSUE TEK QUICK GUIDE 011b-Linfo-CPTH-HIS-TISSUE-TEK USER GUIDE LEVEL SENSOR 102-Linfo-CPTH-HIS-ASP6025 USERMANUAL Using Manual procedures or automated using Leica Peloris I, Leica Peloris II, Leica ASP 6025 and Sakura Tissue-Tek VIP6 Assessment Manager: MP Page 1 of 7

2 Formalin fixed paraffin embedded blocks of processed tissue Decalcification LP-CPTH-HIS-DECAL LP-CPTH-PER-LAB EQUIPMENT Using Milestone bone station and orbit LS agitator Tissue Embedding SOPs : LP-CPTH-HIS-EMBEDDING 055-LI-CPTH-HIS-ALOPECIA EMBEDDING Using Sakura Tissue Embedding Centre and Sakura Tissue Cryo Plate Tissue Sectioning LP-CPTH-HIS-PRODUCTION OF SECTIONS LP-CPTH-PER-LAB LP-CPTH-HIS-MOLECULAR PATHOLOGY LP-CPTH-HIS-RESEARCH 039-LI-CPTH-HIS-MICROTOMY Using Rotary microtomes, Leica RM2235 and RM2135 Assessment Manager: MP Page 2 of 7

3 Fresh frozen tissue FFPE slides (Formalin fixed paraffin embedded) tissue on glass slides FFPE slides (Formalin fixed paraffin embedded), frozen sections and slides prepared from fluids Routine morphological staining Detection of: Basophilic and eosinophilic structures Cryotomy LP-CPTH-HIS-FROZENS LP-CPTH-HIS-REPORTING 065-LI-CPTH-HIS-CRYOTOMY 105c-Linfo-CPTH-HIS-FROZEN AREA ST4020 Using Leica CM1510S and CM 1900 cryostats Interpretive/diagnostic microscopy SOP: LP-CPTH-HIST-REPORTING LP-CPTH-HIS-PRODUCTION OF SECTIONS LP-CPTH-HIS-STAINING 058-LI-CPTH-HIS- COVERSTAINER 105a-Linfo-CPTH-HIS-LINEAR STAINER SET UP065-LI-CPTH- HIS-CRYOTOMY 105c-Linfo-CPTH-HIS-FROZEN AREA ST4020 Routine morphological staining using Dako Artisan coverstainer, Leica Linear Stainer ST4040 (routine), ST4020 (frozen) and Leica CV5030 coverslipper with Haematoxylin and Eosin stain Assessment Manager: MP Page 3 of 7

4 FFPE slides and slides prepared from fluid specimens Special staining Detection of: Acid Mucin Acid and neutral mucin Mast cells Helicobacter Reticulin Fibres Fungi Tubercle bacilli Glycogen, Fungi, Muicin Haemosiderin and ferric salts Spirochetes and Helicobacter LP-CPTH-HIS-ARTISAN LP-CPTH-HIS-STAINING LP-CPTH-HIS-EQA/IQA Automated staining using Dako Artisan and the following stains: Alcian Blue ph 2.5 (AB) Alcian Blue PAS (ABPAS) Diastase Periodic Acid Schiff Giemsa Gordon and Sweet (Retic) for liver & BMTs Grocott s Hexamine Silver (GHS) Ziehl-Neelsen (ZN) Periodic Acid Schiff (PAS) Perl s Prussian Blue Warthin and Starry Assessment Manager: MP Page 4 of 7

5 FFPE slides and slides prepared from fluid specimens (cont d) Special staining (cont d) Detection of: (cont d) General morphology Mast cells Elastin Bile salts Gram ve and +ve bacteria Collagen Amyloid Muscle and collagen Melanin Connective tissue Lipids Elastin collagen, muscles Muscle Copper associated protein LP-CPTH-HIS-SPECIAL STAINS LP-CPTH-STAINING LP-CPTH-HIS-IQA/EQA Manual hand staining using the following stains: Haematoxylin and Eosin (H&E) Azure A Elastin H&E Fouchet Reaction Gram Haematoxylin Van Gieson (HVG) Congo Red Martius Scarlet Blue (MSB) Masson Fontana Masson Trichrome Oil Red O Miller s Elastic Van Gieson (EVG) Phosphotungstic Acid Haematoxylin Rhodanine Assessment Manager: MP Page 5 of 7

6 FFPE slides and slides prepared from fluid specimens (cont d) Special staining (cont d) Detection of: (cont d) Hepatitis B antigen, elastin fibres and copper associated protein. Cryptococcus Calcium salts Acid fast bacilli (Leprosy) Nuclear detail Pigment removal LP-CPTH-HIS-SPECIAL STAINS LP-CPTH-STAINING LP-CPTH-HIS-IQA/EQA Manual hand staining using the following stains: Orcein Southgate s Mucicarmine Von Kossa Wade Fite Celestine Blue Melanin Assessment Manager: MP Page 6 of 7

7 Ascitic fluid, bile, biliary brushings, bladder washing, bronchial brushings and washings, bronchoalveolar lavage, common bile duct brushings, cell block, cerebrospinal fluid, cystic fluid, endobronchial ultra-sound guided sample (EBUS) fine needle aspirations, hydrocele fluid, joint/ synovial fluid, kidney washings, mediastinal fluid, needle washings, nipple discharge, oesophageal brushings, ovarian cyst fluid, pancreatic brushings, pericardial, peritoneal and pleural fluid, sputum, urine, ureteric washings/ brushings Stained Slides prepared in-house FNA samples only Non-Gynae Cytopathological examination activities for the purposes of clinical diagnosis Examination of cellular material in order to identify or exclude morphological and cytological abnormalities Cells (nuclei and cytoplasm) and micro organisms END LP-CPTH-CYT-CELL BLOCK LP-CPTH-CYT-BRUSH LP-CPTH-CYT-FNA LP-CPTH-CYT-RS LP-CPTH-CYT-URINE LP-CPTH-CYT-FLUID LP-CPTH-CYT-EQUIP LP-CPTH-CYT-GEN PROC LP-CPTH-CYT-NG PROCESS Cytospin and direct preparations of samples SOP LP-CPTH-CYT-STAINING for routine morphological staining using Leica Autostainer XL with the following stains: Papanicoloau May-Grunwald Giemsa RAL 555 using manual method Interpretive/diagnostic microscopy (qualitative analysis) LP-CPTH-CYT-REPORTING SPECIMEN LP-CPTH-GEN-MICROSCOPE CPTH-GEN-Using the multiheader microscope Assessment Manager: MP Page 7 of 7

Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK 142-144 New Cavendish Street W1W 6YF Contact: Mr Tel: +44 (0) 20 7299 4490 Fax: +44 (0)20 7299 4491 E-Mail: Neil.Barrett@unilabs.com Website: