Supplemental Table 1. MicroRNAs (mirnas) up- or downregulated in various

Transcription

1 Supplemental Table 1. MicroRNAs (mirnas) up- or downregulated in various tumors relative to normal tissues mirna Up/down Cancer Reference Up Colon cancer 1 Down Breast cancer 2 Down Prostate cancer 3 Down Hepatocellular cancer 4 let-7 Down Gastric tumor 5 Up Uterine leiomyoma 6 Up Pancreatic cancer 7 Up Hepatocelluar carcinoma 8 Down Lung cancer 9 Down Lung cancer 10 Down Breast cancer 11 mir-9 Up Breast cancer 12 Down Neuroblastoma 13 mir-10b Up Breast cancer 12 Up Glioblastoma 14 mir-17-5p Up Neuroblastoma 15 Down Breast cancer 16 mir-18 Up Hepatocellular carcinoma 17 Up Breast cancer 2 Up Colorectal cancer 18 Up Ovarian cancer 19 Up Hepatocellular cancer 20 Up Cervical cancer 21 Up Pancreatic cancer 22 Up Chronic lymphocytic leukemia 23 Up Uterine leiomyoma 6 mir-21 Up Pancreatic cancer 7 Down Pituitary adenomas 24 Up Breast tumor 25 Up Pancreatic tumor 26 Up Hepatocelluar carcinoma 8 Up Cholangiocarcinoma 27 Up Lung cancer 28 Up Breast cancer 29 Up Glioblastoma 30 mir-23a Up Pancreatic tumor 26

2 mir-23b Up Uterine leiomyoma 6 mir-23 Up Hepatocelluar carcinoma 8 mir-26a Down Thyroid cancer 31 mir-26b Up Pancreatic tumor 26 mir-27a Up Breast cancer 32 mir-27b Down Breast cancer 33 mir-29s Down Cholangiocarcinoma 34 mir-30a Down Thyroid cancer 31 mir-30d Down Thyroid cancer 31 mir-31 Up Colorectal cancer 35 Down Lung cancer 36 Down Neuroblastoma 37 mir-34s Down Pancreatic cancers 38 Up Renal cell carcinoma 39 Up Colon cancers 40 Up Hepatocellular carcinoma 20 mir-92 Up Neuroblastoma 15 Down Chronic lymphocytic leukemia 23 mir-96 Up Colorectal cancer 35 mir-100 Up Pancreatic cancer 7 mir-103 Up Pancreatic tumor 26 mir-107 Up Pancreatic tumor 7 mir-122a Down Hepatocellular cancer 4 mir-122 Down Hepatocelluar carcinoma 8 mir-124a Down Colon cancer, lung cancer 41 mir-125a Down Neuroblastoma 13 Up Prostate cancer 42 Down Prostate cancer 3 Down Ovarian cancer 19 mir-125b Down Thyroid cancer 31 Down Neuroblastoma 13 Up Pancreatic cancer 7 Down Breast cancer 29 mir-126* Down Lung cancer 28 mir-128 Down Glioblastoma 14 mir-130 Up Hepatocelluar carcinoma 8 mir-133b Down Colorectal cancer 35 mir-135b Up Colorectal cancer 35 mir-140 Down Ovarian cancer 19 mir-141 Up Ovarian cancer 19 Down Pituitary adenomas 24 mir-143 Down B cell maligancies 43 Down Cervical cancer 21

3 Down Colorectal neoplasia 44 mir-144 Down Pituitary adenomas 24 Down B cell maligancies 43 Down Prostate cancer 3 mir-145 Down Ovarian cancer 19 Down Colorectal cancer 35 Down Breast cancer 29 Down Colorectal neoplasia 44 mir-146b Up Papillary thyroid carcinoma 45 mir-149 Up Pituitary adenomas 24 mir-150 Up Chronic lymphocytic leukemia 23 mir-155 Up Breast cancer 12 Up Pancreatic cancer 22 Up Chronic lymphocytic leukemia 23 Up Pancreatic cancer 7 Down Pancreatic tumor 26 Up Hodgkin's lymphomas, diffuse large B cell lymphoma, primary mediastinal B cell lymphoma 46 Up Diffuse large B cell lymphoma 47 Up Burkitt lymphoma 48 Up Pancreatic cancer 22 mir-181a Up Pancreatic cancer 7 Down Glioblastoma 14 Up Colon cancer 1 mir-181b Up Pancreatic cancer 22 Up Papillary thyroid carcinoma 49 Down Glioblastoma 14 mir-181d Up Pancreatic cancer 22 mir-183 Up Colorectal cancer 35 mir-184 Down Neuroblastoma 50 mir-192 Up Pancreatic tumor 26 mir-196a Up Pancreatic ductal adenocarcinoma 51 mir-197 Up Thyroid cancer 52 mir-199a Down Ovarian cancer 19 Down Hepatocellular carcinoma 17 mir- 199a* Down Hepatocellular carcinoma 17 mir-200a Up Ovarian cancer 19 Down Hepatocellular carcinoma 17 mir-200c Up Colon cancer 1 mir-204 Up Pancreatic tumor 26 mir-205 Up Lung cancer 28 mir-211 Up Pancreatic tumor 26 mir-212 Up Pancreatic cancer 7 mir-217 Down Pancreatic ductal adenocarcinoma 51

4 mir-221 mir-222 Up Glioblastoma 53 Up Hepatocellular cancer 4 Up Pancreatic cancer 22 Up Pancreatic cancer 7 Up Papillary thyroid carcinoma 49 Up Papillary thyroid carcinoma 45 Up Glioblastoma 14 Up Glioblastoma 53 Up Pancreatic cancer 22 Up Papillary thyroid carcinoma 49 Down Chronic lymphocytic leukemia 23 Up Papillary thyroid carcinoma 45 mir-224 Up Hepatocellular carcinoma 17 mir-301 Up Pancreatic cancer 7 mir-320 Up Neuroblastoma 15 mir-342 Up Pancreatic tumor 26 mir-346 Up Thyroid cancer 52 mir-372 Up Testicular germ cell tumor 54 mir-373 Up Testicular germ cell tumor 54 mir-375 Down Pancreatic cancer 7 mir-376a Up Pancreatic cancer 7 mir-16-1,- 15a cluster mir-17 cluster mir-106a cluster Down Chronic lymphocytic leukemia 55 Down Chronic lymphocytic leukemia 56 Down Pituitary adenomas 57 Down B-cell chronic lymphocytic leukemias 58 Up Erythroleukemia 59 Up Lung cancer cell line 60 Up Lung cancer 61 Up B cell lymphomas 62 Up T cell leukemia 63

5 Supplemental Table 2. MicroRNAs (mirnas) that affect the properties of cancer cells mirna /- Effect on cell growth Note Reference let-7 Inhibition of cell growth Lung cancer cell lines 10 Inhibition of cell growth G1 arrest A549 lung cancer line or let-7 hepg2 cell line 64 Enhanced cell growth A549 lung cancer line let-7c Inhibition of cell growth G1 accumulation Hepa-1 65 let-7 Inhibition of cell growth Lung cancer cell lines 66 let-7g Inhibition of cell growth let-7a-3 let-7 mir-9, - 125a, - 125b mir-10b mir-16-1,- 15a Enhanced cell growth Increased anchorage independent growth soft agar assay Enhanced cytotoxicity more apoptosis by gemcitabine, 5-FU, camptothecin Inhibition of cell growth decreased brdu incorporation Enhanced cell growth rescue of RA-induced growth arrest Increased migration, invasion, intravasation, metastasis Decreased invasion Increased apoptosis 67 A549 lung cancer line 68 Cholangiocarcinoma cell lines 69 Neuroblastoma 13 Megakaryocytic cell line MEG-01 Inhibition of cell growth adhesion mir-17-5p independent growth Breast cancer 16 Enhanced cell growth mir-17-5p and 20a Inhibition of cell growth increased apoptosis Lung cancer cell line Calu-6 and ACC-LC mir-17-5p, -20 Enhanced cell growth adhesion independent growth, in vitro culture and engrafted tumor 70 mir-20a Increased apoptosis in combination with doxorubicin 71 mir cluster Enhanced cell growth Chronic myeloid leukemia cell line 72 mir cluster Increased angiogenesis Colon cancer model 73 mir cluster Enhanced cell growth Lung cancer cell line 61 Increased invasion, intravasation, metastasis mir-21 Decreased invasion, intravasation, Colorectal cancer 18 metastasis mir-21 Increased apoptosis reduced tumor growth upon inhibition by modified LNA in vivo Glioblastoma 74 mir-21 Enhanced cell growth, increased migration Hepatocellular cancer cell Inhibition of cell growth, decreased cell lines and normal hepatocytes migration and invasion 20 mir-21 Decreased apoptosis upon IL-6 removal Myeloma cell line 75 mir-21 Inhibition of cell growth in vitro culture and engrafted tumor, increased apoptosis Breast tumor 25 mir-21 Decreased cytotoxicity and less apoptosis upon gemcitabine treatment in vitro culture Cholangiocarcinoma

6 Increased cytotoxicity and more apoptosis upon gemcitabine treatment in vitro culture mir-21 Increased apoptosis Glioblastoma cell line 30 mir-26, - Increased apoptosis 210, -107 Decreased apoptosis HIF hypoxia-induced mirnas 76 mir-27a Increased apoptosis, inhibition of cell growth Breast cancer cell lines 32 mir-29a, b, c Inhibition of cell growth in vitro culture and engrafted tumor, increased apoptosis Lung cancer cells 77 mir-29b Increased apoptosis Cholangiocarcinoma cell line Decreased apoptosis TRAIL-induced cell death 34 mir-34a Inhibition of cell growth in vitro culture and engrafted tumor, senescence-like phenotype 40 mir-34a Inhibition of cell growth G1 accumulation 78 mir-34a Inhibition of cell growth increased apoptosis 38 Inhibition of cell growth increased apoptosis mir-34a Decreased apoptosis induced by genotoxic 79 stress mir-34a G1 accumulation 36 mir-34a Inhibition of cell growth increased apoptosis Neuroblastoma cell lines 37 mir-34a, - 34b, -34c Inhibition of cell growth G1 accumulation and senescence 80 mir-34b and -34c Inhibition of cell growth adhesion independent growth 81 mir-98 More resistant to doxorubicin Primary human oral squamous cell carcinoma cell lines 82 mir-106a Transformation of NIH3T3 colony 63 cluster mir-125a, -125b mir-125b formation assay Inhibition of cell growth, decreased migration and invasion Enhanced cell growth Inhibition of cell growth Breast cancer 83 Prostate cancer cell lines 42 mir-125b Inhibition of cell growth Differentiated cell lines 84 mir-125b, Inhibition of cell growth FB-1 cells, thyroid cancer cell 31-26a line mir-143, - Inhibition of cell growth B cell malignancies, Raji cell mir-184 Inhibition of cell growth increased apoptosis Neuroblastoma cell lines 50 mir-197, - Inhibition of cell growth Thyroid cancer mir-221, - Inhibition of cell growth Glioblastoma mir-221, - Inhibition of cell growth G1 block Glioblastoma Enhanced cell growth adhesion independent mir-221, - growth Lncap prostate cancer cell line Inhibition of cell growth adhesion independent growth PC3 prostate cancer cell line Enhanced cell growth mir-221 Papillary thyroid carcinoma 49 Inhibition of cell growth mir-372, - Increased cell growth adhesion independent Testicular germ cell tumor growth, decreased senescence Increased cell growth in vitro culture and engrafted tumor, reduced apoptosis, mir-378 enhanced angiogenesis U87 glioblastoma cell line 87 Inhibition of cell growth

7 EBVencoded BART mirnas Decreased apoptosis Nasopharyngeal carcinoma 88 Phenotypes of cells are described after ectopic expression (denoted as in the second column) or inhibition ( - in the second column) of a mirna. Abbreviations: EBV, Epstein-Barr virus; TRAIL, tumor necrosis factor--related apoptosis-inducing ligand.

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