Cancer cell movement study using 2D and 3D In Vitro models
|
|
|
- Pierce Baker
- 6 years ago
- Views:
Transcription
1 Cancer cell movement study using 2D and 3D In Vitro models by Ehsanul HOQUE APU Cancer and Translational Medicine Research Unit, Medical Research Center Oulu (MRC Oulu)
2 Squamous cell carcinoma (SqCC) Squamous cell carcinoma (SqCC) is the most common variety of oral cancers and most frequently appears in the tongue. Buccal SqCC is most commonly diagnosed in Southeast Asian and Indian populations because of the betel nut use. Worse survival and higher recurrence rate compared with SqCC arising from other sites of the oral cavity. (Bello et al, 2010; DeConde et al, 2012) Buccal SCC,Source: IARC
3 Invasion and metastases of SqCC The initial steps of local invasion: 1. Activation of signaling pathways that control cytoskeletal dynamics in tumor cells. 2. Turnover of cell matrix and cell-cell junctions, followed by active tumor cell migration and invasion into the adjacent tissue. Metastasis occurs when invading tumor cells engage with blood and lymph vessels. (Friedl and Wolf, 2003; Sahai, 2007)
4 Tumor microenvironment (TME) and different 3D In Vitro TME models.
5 Tumor microenvironment (TME) Non- transformed stromal elements within the tumor. These elements include: Blood vessels Cancer associated fibroblasts (CAFs) Inflammatory cells Extracellular matrix (ECM) molecules. Cancer progression, affecting the growth, invasion and spread of the tumor. (Madar et al., 2013; Hanahan and Weinberg, 2011)
6 Myoma 3D model Human Uterine Bening Smooth Muscle Neoplasm Uterus
7 Myogel Human leiomyoma derived solution/gel prepared according to the preparation of Matrigel (Kibbey 1994). Matrigel is a Commercial Basement membrane matrix from mouse EHS sarcoma. Myogel preparation Frozen Myoma tissue Cryogenic grinding Powdered form Suspended in NaCl buffer Centrifugation Pellet homogenized Protein assay Protein concentration Absorbance (590 nm) Store at Minus 20 degrees Celsius
8 Good to know Myogel is less stiffer and viscous then Matrigel. Myogel-Collagen shows increased stiffness and viscosity comparing with Myogel, but lower than Matrigel. Myogel-Collagen shows constant firmness after polymerization same as Matrigel. Myogel is less stable, static in 3D conditions than both Myogel-Collagen and Matrigel.
9 2D Migration pattern analyses of SqCC/Y1 lines using IncuCyte Vect Ct FL BB ZB SqCC/Y1 (BB)
10 3D Sandwich culture using three different ECM SqCC/Y1 (RFP) cell lines were used. Fill the inner well with a gelmatrix. Seed cells on top of the polymerized gel. Imbed the cells with gel in the upper well Cells in ECM gel Lower ECM gel Upper ECM gel Lower ECM gel Medium
11 3D Volume in Maximum intensity projection x y z
12 z 3D Volume Surface rendered x y y z x
13 3D Surface rendering shows SqCC/Y1 (BB) cell line movement in Z axis directions Myogel-collagen matrix z x z Matrigel-collagen matrix
14 Velocity (μm/hour) 3D directional velocity (mean speed) in different SQCC/Y1 CELLS 10 P = 0, P = 0,03 4 P = 0, V FL BB ZB V FL BB ZB V FL BB ZB V FL BB ZB
15 Accumulated distance is the total distance travelled in 3D Accumulated distance (μm) *** ** *** *** *** *** ** ** *** 20 0 V FL BB ZB V FL BB ZB V FL BB ZB V FL BB ZB
16 Euclidean distance (μm) Euclidean distance is the mean distance between the origin and end point in 3D *** *** ** ** *** *** ** ** 0 V FL BB ZB V FL BB ZB V FL BB ZB V FL BB ZB
17 Results (Coculture, 2:1) Velocity (μm/hour) CAF co-culture CAF BB Vect ZB
18 Results (Coculture, 2:1) Velocity (μm/hour) NF co-culture NF Vect BB ZB
19 Velocity (μm/hour) 3D directional velocity (Coculture, 2:1) Velocity (μm/hour) Velocity (μm/hour) NF CAF SqCC/Y1 (Vect)-NF SqCC/Y1 (BB)-NF SqCC/Y1 (ZB)-NF Vect BB ZB Vect BB ZB SqCC/Y1 (Vect)-CAF SqCC/Y1 (BB)-CAF SqCC/Y1 (ZB)-CAF SqCC/Y1 Vect BB ZB Vect BB ZB
20 Myogel Human ECM model Transparent. Readily visualized with standard staining, live cell 3D imaging & high resolution microscopy. Outstanding biocompatibility. Provides ideal 3D In Vivo like tumor environment. Myogel-collagen Matrix Offers an excellent TME matrix for cancer cell In Vitro invasion experiments. Sandwich 3D Assay With live cell imaging & mathematical analyis to study cancer cell invasion more precisely.
21 Research Group of Cancer Research and Translational Medicine, University of Oulu Professor Tuula Salo Dr Meeri Sutineen Dr Johanna Korvala Dr Maija Risteli Biocenter Oulu Dr Lauri Eklund Light microscopy core facility, Biocenter Oulu. Dr Veli-Pekka Ronkainen Dept. of Computer Science Engineering; University of Oulu. Saad Ullah MSc Queen Mary, University of London Dr Hong Wan
Challenges for 3D Cancer Cell Culture On Basement Membrane. AACR, 2009 Presented by Hynda K. Kleinman, PhD
Management of Disease Through DNA Repair Challenges for 3D Cancer Cell Culture On Basement Membrane AACR, 2009 Presented by Hynda K. Kleinman, PhD ams biotechnology (europe) ltd info@amsbio.com www.amsbio.com
Cancer Biology Course. Invasion and Metastasis
Cancer Biology Course Invasion and Metastasis 2016 Lu-Hai Wang NHRI Cancer metastasis Major problem: main reason for killing cancer patients, without it cancer can be cured or controlled. Challenging questions:
Neoplasia literally means "new growth.
NEOPLASIA Neoplasia literally means "new growth. A neoplasm, defined as "an abnormal mass of tissue the growth of which exceeds and is uncoordinated with that of the normal tissues and persists in the
In vitro scratch assay: method for analysis of cell migration in vitro labeled fluorodeoxyglucose (FDG)
In vitro scratch assay: method for analysis of cell migration in vitro labeled fluorodeoxyglucose (FDG) 1 Dr Saeb Aliwaini 13/11/2015 Migration in vivo Primary tumors are responsible for only about 10%
Using Histoindex Genesis 200 imaging technology to analyse the Pattern of ECM Deposition in Human Breast Cancer Tissue
Using Histoindex Genesis 200 imaging technology to analyse the Pattern of ECM Deposition in Human Breast Cancer Tissue Introduction to technology High Resolution Detects fine collagen fibers up to 0.1µm
Supplementary Figures for
mirns regulate s Supplementary igures for MicroRNs Reprogram Normal ibroblasts into Cancer ssociated ibroblasts in Ovarian Cancer nirban K. Mitra, Marion Zillhardt, Youjia Hua, Payal iwari, ndrea E. Murmann,
Cervical Cancer: 2018 FIGO Staging
Cervical Cancer: 2018 FIGO Staging Jonathan S. Berek, MD, MMS Laurie Kraus Lacob Professor Stanford University School of Medicine Director, Stanford Women s Cancer Center Senior Scientific Advisor, Stanford
Trevigen s Extracellular Matrix Proteins for Triculture of Breast Cancer Spheroids, Endothelial Tubules, and Human Mesenchymal Stem Cells
Trevigen s Extracellular Matrix Proteins for Triculture of Breast Cancer Spheroids, Endothelial Tubules, and Human Mesenchymal Stem Cells Gabriel Benton, Ph.D. and Jay George, Ph.D., Trevigen, Inc. Gaithersburg,
SUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb3021 Supplementary figure 1 Characterisation of TIMPless fibroblasts. a) Relative gene expression of TIMPs1-4 by real time quantitative PCR (RT-qPCR) in WT or ΔTimp fibroblasts (mean ±
ONCOLOGY. Csaba Bödör. Department of Pathology and Experimental Cancer Research november 19., ÁOK, III.
ONCOLOGY Csaba Bödör Department of Pathology and Experimental Cancer Research 2018. november 19., ÁOK, III. bodor.csaba1@med.semmelweis-univ.hu ONCOLOGY Characteristics of Benign and Malignant Neoplasms
Supplementary Figure 1: GFAP positive nerves in patients with adenocarcinoma of
SUPPLEMENTARY FIGURES AND MOVIE LEGENDS Supplementary Figure 1: GFAP positive nerves in patients with adenocarcinoma of the pancreas. (A) Images of nerves stained for GFAP (green), S100 (red) and DAPI
Test Bank for Robbins and Cotran Pathologic Basis of Disease 9th Edition by Kumar
Link full download:https://getbooksolutions.com/download/test-bank-for-robbinsand-cotran-pathologic-basis-of-disease-9th-edition-by-kumar Test Bank for Robbins and Cotran Pathologic Basis of Disease 9th
3D Tissue Models. Simple, Low Cost Fabrication. Simple, Robust Protocols
3D Tissue Models SynVivo is a physiological, cell-based microfluidic platform that provides a morphologically and physiologically realistic microenvironment allowing real-time study of cellular behavior,
Icd 10 code metastatic adenocarcinoma endometrial
Icd 10 code metastatic adenocarcinoma endometrial 1-10-2017 Free, official coding info for 2018 ICD-10-CM D07.0 - includes detailed rules, notes, synonyms, ICD-9-CM conversion,. 2018 ICD-10-CM Diagnosis
Jianhua Qin. Dalian Institute of Chemical Physics Chinese Academy of Sciences
Jianhua Qin Dalian Institute of Chemical Physics Chinese Academy of Sciences Outline What and why microfluidics? Cancer microenvironment on chip Multicellular organism on chip Summary and futures Microfluidics
Prepared By Student. Dania Abed Al-majeed. Rahma Raad Hanna. Balqees Mohammed Aasim. Dania Hisham. Rasha Rafiee
Prepared By Student Rahma Raad Hanna Balqees Mohammed Aasim Dania Hisham Dania Abed Al-majeed Rasha Rafiee Epithelia Epithelia can be derived from ectoderm, mesoderm or endoderm -ectoderm gives rise to
Quantitative Data Analysis Assignment Sample Newessays.co.uk
Absorbance Quantitative Data Analysis Assignment Sample Newessays.co.uk Part A: Results of the Study Is there a difference of curve profile between the MTT assay and the cell number? What do the different
Overview of methodology, tools and reagents for evaluating cell proliferation and invasion using multicellular tumor spheroids.
The Next Step in the Evolution of 3D Culture: Utilizing Extracellular Matrix to Enhance Multicellular Tumor Spheroid Models for Proliferation and Invasion Overview of methodology, tools and reagents for
Tumor microenvironment Interactions and Lung Cancer Invasiveness. Pulmonary Grand Rounds Philippe Montgrain, M.D.
Tumor microenvironment Interactions and Lung Cancer Invasiveness Pulmonary Grand Rounds Philippe Montgrain, M.D. February 26, 2009 Objectives Review epithelial mesenchymal transition (EMT), and its implications
Icd 10 uterine leiomyosarcoma
Icd 10 uterine leiomyosarcoma Free, official coding info for 2018 ICD - 10 -CM C55 - includes detailed rules, notes, synonyms, ICD -9-CM conversion, index and annotation. Histologically confirmed nonresectable
Effect of a nutrient mixture on the localization of extracellular matrix proteins in HeLa human cervical cancer xenografts in female nude mice
Effect of a nutrient mixture on the localization of extracellular matrix proteins in HeLa human cervical cancer xenografts in female nude mice Publication from the Dr. Rath Research Institute Experimental
Most abundant and widely distributed tissues in the body Binds, support, and strengthen body tissues, protect and insulate internal organ, serve as
Connective tissue Most abundant and widely distributed tissues in the body Binds, support, and strengthen body tissues, protect and insulate internal organ, serve as major transport system, compartmentalizes
Cancer Biology Dynamical Cell Systems
The Institute of Cancer Research PHD STUDENTSHIP PROJECT PROPOSAL PROJECT DETAILS Project Title: SUPERVISORY TEAM Primary Supervisor: The forces behind pancreatic cancer; and changing them as a therapeutic
stem cell products Basement Membrane Matrix Products Rat Mesenchymal Stem Cell Growth and Differentiation Products
stem cell products Basement Membrane Matrix Products Rat Mesenchymal Stem Cell Growth and Differentiation Products Stem Cell Qualified Extracellular Matrix Proteins Stem cell research requires the finest
Chapter 4 :Organization & Regulation of Body Systems
Chapter 4 :Organization & Regulation of Body Systems 4.1 Types of tissues What is a tissue? A collection of cells of the same type that perform a common function There are 4 major tissue types in the body:
Disclosure. Acknowledgement. What is the Best Workup for Rectal Cancer Staging: US/MRI/PET? Rectal cancer imaging. None
What is the Best Workup for Rectal Cancer Staging: US/MRI/PET? Zhen Jane Wang, MD Assistant Professor in Residence UC SF Department of Radiology Disclosure None Acknowledgement Hueylan Chern, MD, Department
Neoplasia 2018 Lecture 2. Dr Heyam Awad MD, FRCPath
Neoplasia 2018 Lecture 2 Dr Heyam Awad MD, FRCPath ILOS 1. List the differences between benign and malignant tumors. 2. Recognize the histological features of malignancy. 3. Define dysplasia and understand
Neuroradiology Case of the Day
Neuroradiology Case of the Day 76 th CAR Annual Meeting, Montreal, Quebec April 27, 2013 Eugene Yu, MD Assistant Professor of Radiology and Otolaryngology-Head and Neck Surgery Head and Neck Imaging Princess
CD34 + VEGFR-3 + progenitor cells have a potential to differentiate towards lymphatic endothelial cells
CD34 + VEGFR-3 + progenitor cells have a potential to differentiate towards lymphatic endothelial cells Tan YZ et al. J Cell Mol Med. (2014 Mar;18(3):422-33) Denise Traxler-Weidenauer April 2014 Introduction
Icd 10 uterine leiomyosarcoma
Icd 10 uterine leiomyosarcoma Search Aetna considers myomectomy or hysterectomy using power morcellation experimental and investigational for the removal of uterine fibroids because its safety and. Free,
Cell Migration and Invasion Assays INCUCYTE LIVE-CELL ANALYSIS SYSTEM. Real-time automated measurements of cell motility inside your incubator
Cell Migration and Invasion Assays INCUCYTE LIVE-CELL ANALYSIS SYSTEM Real-time automated measurements of cell motility inside your incubator See the whole story Real-time cell motility visualization and
NEOPLASIA-I CANCER. Nam Deuk Kim, Ph.D.
NEOPLASIA-I CANCER Nam Deuk Kim, Ph.D. 1 2 Tumor in the hieroglyphics of the Edwin Smith papyrus (1,600 B.C., Breasted s translation 1930) 3 War on Cancer (National Cancer Act, 1971) 4 Cancer Acts in Korea
The Angio-Ready Assay System
The Angio-Ready Assay System Kevin Grady, B.S. Product Line Business Manager, ATCC Cell Systems Charles Zou, Ph.D. Senior Scientist, ATCC Cell Systems About ATCC Founded in 1925, ATCC is a non-profit organization
Imaging of Gastrointestinal Stromal Tumors (GIST) Amir Reza Radmard, MD Assistant Professor Shariati hospital Tehran University of Medical Sciences
Imaging of Gastrointestinal Stromal Tumors (GIST) Amir Reza Radmard, MD Assistant Professor Shariati hospital Tehran University of Medical Sciences Describe the typical imaging findings of GIST at initial
Biochemistry of Carcinogenesis. Lecture # 35 Alexander N. Koval
Biochemistry of Carcinogenesis Lecture # 35 Alexander N. Koval What is Cancer? The term "cancer" refers to a group of diseases in which cells grow and spread unrestrained throughout the body. It is difficult
Construction of Nephron by Fusion of Adult Glomeruli to Ureteric Buds with Type V Collagen. Yusuke Murasawa, Pi-chao Wang
Construction of Nephron by Fusion of Adult Glomeruli to Ureteric Buds with Type V Collagen Yusuke Murasawa, Pi-chao Wang Abstract Although tissue engineering of artificial organs such as skin or cartilage
Comparison of a Persistent Random Walk to 3D Chemo taxis in. MDA-MB-231 Cancer Cells
Comparison of a Persistent Random Walk to 3D Chemo taxis in MDA-MB-231 Cancer Cells Cameron Thayer-Freeman Advisor: Dr. Bo Sun 2015 Abstract This research specifically examines the collective motion of
Anonymous MIT students MK and NM Natalie Kuldell May 2009
Anonymous MIT students MK and NM Natalie Kuldell 20.020 13 May 2009 Technical Document: oncocures Problem: Dynamic Metastasis Imaging Our project has been designed with the hope of creating a way to detect
IncuCyte Scratch Wound 96-Well Real-Time Cell Migration and Invasion Assays
IncuCyte Scratch Wound 96-Well Real-Time Cell Migration and Invasion Assays Meagan Roddy, Thom Nelson, Daniel M. Appledorn, and Vince Groppi Essen BioScience Ann Arbor, Michigan Introduction Cell migration
Introduction to Basic Oncology
Introduction to Basic Oncology Cancer Cell AHS 102 Med Term Dr. Susie Turner 1/3/13 General Oncology Study of Tumors Neoplasms/Tumors Abnormal growth of new tissue Are either; Benign or Malignant Onc/o
Tumour Structure and Nomenclature. Paul Edwards. Department of Pathology and Cancer Research UK Cambridge Institute, University of Cambridge
Tumour Structure and Nomenclature Paul Edwards Department of Pathology and Cancer Research UK Cambridge Institute, University of Cambridge Malignant Metastasis Core idea of cancer Normal Cell Slightly
Examining the relationships between tumor cell motility type, cell migration rate, & matrix pore size in a variety of 3D matrix models
CELLULAR BIOENGINEERING, MAY 2008 1 Examining the relationships between tumor cell motility type, cell migration rate, & matrix pore size in a variety of 3D matrix models Victoria Hsiao & Melissa Yu I.
number Done by Corrected by Doctor Maha shomaf
number 17 Done by Ahmad rawajbeh Corrected by أسامة الخضر Doctor Maha shomaf 0 P a g e In this lecture, we are going to: complete the differentiation between benign and malignant tumors. - -start to study
APPLICATION SPECIFIC PROTOCOL ANGIOGENESIS... 1 TABLE OF CONTENTS... 1 MONOLAYER FORMATION... 2 OPTION 1: APPLICATION OF ANGIOGENIC STIMULI...
APPLICATION SPECIFIC PROTOCOL ANGIOGENESIS AIM 3D Cell Culture Chips offer a new perspective in studying angiogenesis by allowing the growth of new vascular sprouts in a 3D matrix from a pre-existing endothelial
uterine cancer endometrial cancer
2018 ICD-10-CM Diagnosis Code. Adenocarcinoma of endometrium ; Cancer of the. (mucous membrane that lines the endometrial cavity). ICD-10-CM C54.1 is grouped. Home ICD 9 Codes Endometrial Cancer ICD 9
A case of pedunculated intraperitoneal leiomyoma
Jichi Medical University Journal Chio Shuto Kuniyasu Soda Takayoshi Yoshida Fumio Konishi Abstract We report a very rare case of a pedunculated intraperitoneal leiomyoma in the parietal peritoneum of the
Cancer arises from the mutation of a normal gene. A factor which brings about a mutation is called a mutagen.
Cancer Single cells divide by mitosis to form many cells. This cells undergo physical and chemical changes in order to perform specific functions. (we say the cells have Differentiated) in this way we
PRINCESS MARGARET CANCER CENTRE CLINICAL PRACTICE GUIDELINES GYNECOLOGIC CANCER VULVAR
PRINCESS MARGARET CANCER CENTRE CLINICAL PRACTICE GUIDELINES GYNECOLOGIC CANCER VULVAR Last Revision Date July 2015 1 Site Group: Gynecologic Cancer Vulvar Author: Dr. Stephane Laframboise 1. INTRODUCTION
Invasion And Metastasis. Wirsma Arif Harahap Surgical Oncologist Surgery Department Andalas Medical Schoool
Invasion And Metastasis Wirsma Arif Harahap Surgical Oncologist Surgery Department Andalas Medical Schoool Biology of tumor growth The natural history of malignant tumors can be divided into four phase:
Essentials of Clinical MR, 2 nd edition. 73. Urinary Bladder and Male Pelvis
73. Urinary Bladder and Male Pelvis Urinary bladder carcinoma is best locally staged with MRI. It is important however to note that a thickened wall (> 5 mm) is a non-specific finding seen in an underfilled
A neoplasm is defined as "an abnormal tissue proliferation, which exceeds that of adjacent normal tissue. This proliferation continues even after
NEOPLASIA Neoplasia is a very important topic in pathology because neoplasms are both common and serious diseases. A neoplasm literally means a new growth, and this term is used interchangeably with a
Epithelium. Four primary tissue types:
Epithelium Four primary tissue types: Epithelial (covering) Connective (support) Nervous (control) Muscular (movement) Smooth muscle Cardiac muscle Skeletal muscle 1 Epithelial Tissue Features Epithelial
Vision Health: Conditions, Disorders & Treatments EYELID DISORDERS
Vision Health: Conditions, Disorders & Treatments EYELID DISORDERS There are a number of disorders that can affect the eyelid. Entropion Entropion is an inward turning of the eyelid and lashes toward the
Tissues 10/21/2016. Epithelial Tissue
Tissues This is a generalized cell diagram. It shows the anatomy of a cell, but most cells do not actually look like this. Cells can have a wide variety of shapes and sizes, depending on their function.
Most mammalian cells are located in tissues where they are surrounded by a complex extracellular matrix (ECM) often referred to as connective tissue.
GLYCOSAMINOGLYCANS Most mammalian cells are located in tissues where they are surrounded by a complex extracellular matrix (ECM) often referred to as connective tissue. The ECM contains three major classes
RADIOFREQUENCY ABLATION
RADIOFREQUENCY ABLATION ELIZABETH DAVID M D FRCPC VASCULAR A ND INTERVENTIONAL RADIOLOGIST SUNNYBROOK HEALTH SCIENCES CENTRE GIST GASTROINTESTINAL STROMAL TUMORS Stromal or mesenchymal neoplasms affecting
A holistic approach to targeting breast cancer part II: Micronutrient synergy. Presented by: Dr. Neha Shanker DRRI
A holistic approach to targeting breast cancer part II: Micronutrient synergy Presented by: Dr. Neha Shanker DRRI Overview of the previous webinar In the last presentation we talked about: Increase in
Test Bank for Robbins and Cotran Pathologic Basis of Disease 9th Edition by Kumar
Link full download: http://testbankair.com/download/test-bank-for-robbins-cotran-pathologic-basis-of-disease-9th-edition-bykumar-abbas-and-aster Test Bank for Robbins and Cotran Pathologic Basis of Disease
Ocular Neoplasia What s Common? What s New? Richard R Dubielzig
Ocular Neoplasia What s Common? What s New? Richard R Dubielzig Orbit 288 6% Tumors of the globe make up 3225 out of 6110 total neoplasms = 53%. Tumors of the conjunctiva make up 1192 out of 6110 total
Zebrafish xenograft model of glioblastoma to identify regulatory pathways and agents
Zebrafish xenograft model of glioblastoma to identify regulatory pathways and agents Malignant primary brain tumor in humans Median survival is no longer than 1 year 5 year survival rate is less than 5
SUPPLEMENTARY INFORMATION
DOI:.38/ncb3399 a b c d FSP DAPI 5mm mm 5mm 5mm e Correspond to melanoma in-situ Figure a DCT FSP- f MITF mm mm MlanaA melanoma in-situ DCT 5mm FSP- mm mm mm mm mm g melanoma in-situ MITF MlanaA mm mm
Tissues. How do cells form tissues?
Tissues How do cells form tissues? Using cell junctions Tissues Epithelial tissue Connective tissue Muscle tissue Nervous tissue Epithelial Tissue Closely packed cells in continuous sheets connected by
Brief History. Identification : Past History : HTN without regular treatment.
Brief History Identification : Name : 陳 x - Admission : 94/10/06 Gender : male Age : 75 y/o Chief Complaint : Urinary difficulty for months. Past History : HTN without regular treatment. Brief History
Animal Tissue Culture SQG 3242 Biology of Cultured Cells. Dr. Siti Pauliena Mohd Bohari
Animal Tissue Culture SQG 3242 Biology of Cultured Cells Dr. Siti Pauliena Mohd Bohari The Culture Environment Changes of Cell s microenvironment needed that favor the spreading, migration, and proliferation
The International Federation of Gynecology and Obstetrics (FIGO) updated the staging
Continuing Education Column Revised FIGO Staging System Hee Sug Ryu, MD Department of Obstetrics and Gynecology, Ajou University School of Medicine E - mail : hsryu@ajou.ac.kr J Korean Med Assoc 2010;
Rath, N., and Olson, M. (2016) Regulation of pancreatic cancer aggressiveness by stromal stiffening. Nature Medicine, 22(5), pp. 462-463. There may be differences between this version and the published
Inflammatory Cells and Metastasis
Inflammatory Cells and Metastasis Experimentelle Krebsforschung SS 07 Gerhard Christofori Institute of Biochemistry and Genetics Department of Clinical-Biological Sciences Center of Biomedicine University
performed to help sway the clinician in what the appropriate diagnosis is, which can substantially alter the treatment of management.
Hello, I am Maura Polansky at the University of Texas MD Anderson Cancer Center. I am a Physician Assistant in the Department of Gastrointestinal Medical Oncology and the Program Director for Physician
Principles of Surgical Oncology. Winnie Achilles Tierklinik Hollabrunn Lastenstrasse Hollabrunn
Principles of Surgical Oncology Winnie Achilles Tierklinik Hollabrunn Lastenstrasse 2 2020 Hollabrunn boexi@gmx.de The first surgery provides the best chance for a cure in an animal with a tumor Clinical
أملس عضلي غرن = Leiomyosarcoma. Leiomyosarcoma 1 / 5
Leiomyosarcoma 1 / 5 EPIDEMIOLOGY Exact incidence is unknown, but older studies suggest that leiomyosarcomas comprise approximately 3 percent of soft-tissue sarcomas. Superficial leiomyosarcoma occurs
Targeting the cgmp Pathway to Treat Colorectal Cancer
Thomas Jefferson University Jefferson Digital Commons Department of Pharmacology and Experimental Therapeutics Faculty Papers Department of Pharmacology and Experimental Therapeutics 29 Targeting the cgmp
Recombinant Trypsin, Animal Origin Free
Recombinant Trypsin, Animal Origin Free PRODUCT INFORMATION: BioGenomics r-trypsin powder is ready to use, animal origin free optimized for cell culture applications. It is derived by r-dna technology.
Study on the expression of MMP-9 and NF-κB proteins in epithelial ovarian cancer tissue and their clinical value
Study on the expression of MMP-9 and NF-κB proteins in epithelial ovarian cancer tissue and their clinical value Shen Wei 1,a, Chen Juan 2, Li Xiurong 1 and Yin Jie 1 1 Department of Obstetrics and Gynecology,
HOMEWORK RUBRICS MECHANOTRANSDUCTION UNIT: HOMEWORK #1 (20 pts towards your grade)
HOMEWORK RUBRICS MECHANOTRANSDUCTION UNIT: HOMEWORK #1 (20 pts towards your grade) 1. Mesenchymal stem cells (MSC) cultured on extracellular matrices with different stiffness exhibit diverse lineage commitment
Mousa. Israa Ayed. Abdullah AlZibdeh. 0 P a g e
1 Mousa Israa Ayed Abdullah AlZibdeh 0 P a g e Breast pathology The basic histological units of the breast are called lobules, which are composed of glandular epithelial cells (luminal cells) resting on
1.The metastatic cascade. 2.Pathologic features of metastasis. 3.Therapeutic ramifications
Metastasis 1.The metastatic cascade 2.Pathologic features of metastasis 3.Therapeutic ramifications Sir James Paget (1814-1899) British Surgeon/ Pathologist Paget s disease of bone Paget s disease of the
Vascular Mechanobiology: growth and remodeling in the aorta in health and disease
Vascular Mechanobiology: growth and remodeling in the aorta in health and disease Dr.-Ing. Christian J. Cyron Technical University of Munich funded by the German Research Foundation (Emmy-Noether Grant
What is endometrial cancer?
Uterine cancer What is endometrial cancer? Endometrial cancer is the growth of abnormal cells in the lining of the uterus. The lining is called the endometrium. Endometrial cancer usually occurs in women
Endometrial adenocarcinoma icd 10 code
Endometrial adenocarcinoma icd 10 code Gogamz Menu Cancer of the endometrium, adenocarcinoma ;. (mucous membrane that lines the endometrial cavity). ICD - 10 -CM C54.1 is grouped within. ICD-10 -CM Diagnosis
malignant polyp Daily Challenges in Digestive Endoscopy for Endoscopists and Endoscopy Nurses BSGIE Annual Meeting 18/09/2014 Mechelen
Plan Incidental finding of a malignant polyp 1. What is a polyp malignant? 2. Role of the pathologist and the endoscopist 3. Quantitative and qualitative risk assessment 4. How to decide what to do? Hubert
Female Genital Tract Lab. Dr. Nisreen Abu Shahin Assistant Professor of Pathology University of Jordan
Female Genital Tract Lab Dr. Nisreen Abu Shahin Assistant Professor of Pathology University of Jordan Ovarian Pathology A 20-year-old female presented with vague left pelvic pain. Pelvic exam revealed
Imaging in gastric cancer
Imaging in gastric cancer Gastric cancer remains a deadly disease because of late diagnosis. Adenocarcinoma represents 90% of malignant tumors. Diagnosis is based on endoscopic examination with biopsies.
Sheet #9. Dr. Heba Kalbouneh. Dr. Heba Kalbouneh. Dr. Heba Kalbouneh
Sheet #9 Dr. Heba Kalbouneh Dr. Heba Kalbouneh Dr. Heba Kalbouneh Elastic fibers The main function of elastic fibers is to provide elasticity. In other words these fibers are able to restore the original
DUSTURBANCES OF GROWTH. MLS Basic histological diagnosis MLS HIST 422 Semester 8- batch 7 L8 Uz: Musa
DUSTURBANCES OF GROWTH MLS Basic histological diagnosis MLS HIST 422 Semester 8- batch 7 L8 Uz: Musa Agnesia: means complete absence of an organ (Kidney). Aplasia: s defined in general as "defective development
Cancer and Oncogenes Bioscience in the 21 st Century. Linda Lowe-Krentz October 11, 2013
Cancer and Oncogenes Bioscience in the 21 st Century Linda Lowe-Krentz October 11, 2013 Just a Few Numbers Becoming Cancer Genetic Defects Drugs Our friends and family 200 180 160 140 120 100 80 60 40
Practical Histology. Lab 3: Connective tissue
Practical Histology Lab 3: Connective tissue Connective tissues Connective tissue provides structural support for the body by binding cells and tissues together to form organs. It also provides metabolic
I TESSUTI: Dott.ssa Liliana Belgioia Università degli Studi di Genova
I TESSUTI: 1. Repair, Radiosensitivity, Recruitment, Repopulation, Reoxygenation 2. Acute and chronic hypoxia 3. Tissue microenvironment and tissue organization Dott.ssa Liliana Belgioia Università degli
Effect of BD Matrigel Matrix Overlay and BD Matrigel Matrix Thin Coat on CYP450 Activities in Cryo Human Hepatocytes. Rongjun Zuo.
Effect of BD Matrigel Matrix Overlay and BD Matrigel Matrix Thin Coat on CYP450 Activities in Cryo Human Hepatocytes Rongjun Zuo November 10, 2010 Topics Overview of Hepatocyte Products P450 Induction
They cells can not function death.
Jenna Hellack Jan 2001 Tissues What do you think happens when the cells use up their food and oxygen before there is time to replenish it? They cells can not function death. Blood Cell Cancer cell Plant
Introduction. Table 1. Metastatic Tumor Formation
CellPlayer 96-Well Kinetic Cell Migration and Invasion Assays Meagan Roddy, Thom Nelson, Daniel M. Appledorn, and Vince Groppi Essen BioScience Ann Arbor, Michigan Introduction Cell migration is a multistep
NEOPLASIA. 3. Which of the following tumour is benign a. Chondrosarcoma b. Osteochondroma c. Chondroblastoma d. Ewing s tumour e.
NEOPLASIA 1. malignant neoplasms a. are independent of hormonal influence b. are always composed of homogenous cell lines c. arise from differentiated cells by a process of anaplasia d. display abnormal
Staging and Treatment Update for Gynecologic Malignancies
Staging and Treatment Update for Gynecologic Malignancies Bunja Rungruang, MD Medical College of Georgia No disclosures 4 th most common new cases of cancer in women 5 th and 6 th leading cancer deaths
Anatomy and Physiology Tissue Review
Anatomy and Physiology Tissue Review OVERVIEW Histology practicals can be rough, especially when access to slides is limited to the lab period. This resource provides an opportunity to learn or review
Suppl Video: Tumor cells (green) and monocytes (white) are seeded on a confluent endothelial
Supplementary Information Häuselmann et al. Monocyte induction of E-selectin-mediated endothelial activation releases VE-cadherin junctions to promote tumor cell extravasation in the metastasis cascade
Virtual Melanoma: When, Where and How Much to Cut Yang Kuang, Arizona State University
Virtual Melanoma: When, Where and How Much to Cut Yang Kuang, Arizona State University Based on: Eikenberry S, Thalhauser C, Kuang Y. PLoS Comput Biol. 2009, 5:e1000362. Mathematical Modeling of Melanoma
Modelling Cancer Cell Migration and Invasion Using Novel Technology for Three Dimensional Cell Culture. Introduction
Modelling Cancer Cell Migration and Invasion Using Novel Technology for Three Dimensional Cell Culture Application Note 3 Introduction Cancer is characterised by unregulated and abnormal cell growth in
A Study Of Morphometrical Differences Between Normal Mucosa, Dysplasia, Squamous Cell Carcinoma & Pseudoepitheliomatous Hyperplasia Of The Oral Mucosa
IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS) e-issn: 2278-3008. Volume 5, Issue 4 (Mar. Apr. 2013), PP 66-70 A Study Of Morphometrical Differences Between Normal Mucosa, Dysplasia, Squamous
The Angiopoietin Axis in Cancer
Ang2 Ang1 The Angiopoietin Axis in Cancer Tie2 An Overview: The Angiopoietin Axis Plays an Essential Role in the Regulation of Tumor Angiogenesis Growth of a tumor beyond a limiting size is dependent upon
Endometrial Stromal Sarcoma
May 26, 2011 By Sushila Ladumor, MD [1] Endometrial stromal sarcoma (ESS) is a rare malignant tumor of the endometrium, occurring in the age group of 40-50 years. History The 50-year-old, female patient
Diplomate of the American Board of Pathology in Anatomic and Clinical Pathology
A 33-year-old male with a left lower leg mass. Contributed by Shaoxiong Chen, MD, PhD Assistant Professor Indiana University School of Medicine/ IU Health Partners Department of Pathology and Laboratory