Ten Second DART -MS: Ultra-Fast QC Screening of Foods and Juices with Next Generation DART systems

Transcription

1 Ten Second DART -MS: Ultra-Fast QC Screening of Foods and Juices with Next Generation DART systems IonSense, Inc. 999 Broadway Suite 404 Saugus, MA USA

2 DART-SVP Ionization Source

3 DART -SVP Ion Source with automated sampler

4 Method Development Optimization of Desorption Conditions Rapid detection of Adulterants in Herbal Supplements Rapid Screening for Synthetic AntidiabeticDrug Adulteration in Herbal Dietary Supplements using Direct Ionization in Real Time Mass Spectrometry, Z Zhou, J. Zhang, W. Zhang, Y. Bai, and H. Liu Analyst, in press 2011

5 Optimization of DART Conditions Gas Temp Gas Flow

6 DART MS and MS/MS Triplicate Analysis

7

8 ID-CUBE : Built on DART Technology

9 ID-CUBE Ionization Source

10 1. Spot 5 µl aliquot or powder residue on the OpenSpot Card 2. Let sample dry for easy transport or analyze directly when wet 3. Insert sample cardinto the slot on ID-CUBE Source 4. Set the desired heating setting on ID-CUBE control box and press button for instant desorption

11 Nature s Bounty Omega-3 Fish Oil Positive Ion Mode 250 C NL: 5.37E6 DART-SVP 1-D Bounty Fish Oil 02_POS_ T: [ Relative Abundance [Cholesterol + H H 2 O]+ Elemental composition search on mass m/z= m/z Theo. Mass Delta (ppm) RDB equiv C 27 H C H O N [Cholesterol + H H 2 O]+ Diglycerides Composition 350 C 450 C Triglycerides m/z NL: 1.39E6 DART-SVP 1-D Bounty Fish Oil 02_POS_20110 SB: [ NL: 2.48E5 DART-SVP 1-D Bounty Fish Oil 02_POS_20110 SB: [

12 Relative Abundance Nature s Bounty Omega-3 Fish Oil C C C Negative Ion Mode EPA Elemental composition search on mass DHA m/z NL: 2.12E5 DART-SVP 1-D Bounty Fish Oil 01_NEG_20110 SB: [ m/z= m/z Theo. Mass Delta (ppm) RDB equiv C20H29O C H O N Elemental composition search on mass Composition NL: 6.33E5 DART-SVP 1-D Bounty Fish Oil 01_NEG_20110 Composition SB: [ m/z= m/z Theo. Mass Delta (ppm) RDB equiv C22H31O C H O N NL: 4.91E5 DART-SVP 1-D Bounty Fish Oil 01_NEG_20110 SB: [

13 Relative Abundance Vitamin Shoppe Omega 3 Fish Oil Positive Ion Mode Elemental composition search on mass m/z= m/z Theo. Mass Delta (ppm) RDB equiv. Composition C29H50O C H O Vitamin E as alpha-tocopherol No Diglycerides 250 C 350 C 450 C No Triglycerides m/z NL: 4.73E6 DART-SVP 1-D Shoppe Fish Oil 01_POS_20110 SB: [ NL: 6.11E6 DART-SVP 1-D Shoppe Fish Oil 01_POS_20110 SB: [ NL: 7.27E6 DART-SVP 1-D Shoppe Fish Oil 01_POS_20110 SB: [

14 Vitamin Shoppe Omega 3 Fish Oil C Negative Ion Mode EPA NL: 1.25E5 DART-SVP 1-D Shoppe Fish Oil 01_NEG_20110 SB: [ Relative Abundance C C DHA Elemental composition search on mass m/z NL: 2.03E6 DART-SVP 1-D Shoppe Fish Oil 01_NEG_20110 Composition SB: [ m/z= m/z Theo. Mass Delta (ppm) RDB equiv C20H29O C H O Elemental composition search on mass NL: 2.32E6 DART-SVP 1-D Shoppe Fish Oil Composition 01_NEG_20110 SB: [ m/z= m/z Theo. Mass Delta (ppm) RDB equiv C22H31O C H O

15 Routine analysis of powders The tablet was sliced open and using a standard glass capillary tube a small amount of material was scarped from the inside of the tablet, transferred onto the mesh and directly screened for the FDA regulated API sildenafil.

16 Mexican sildenafil Tablet Scraping Relative Abundance [M+H] + DART Spectrum [M+H] + Elemental Composition Simulation NL: 1.70E4 C 22 H 31 O 4 N 6 S: C 22 H 31 O 4 N 6 S 1 p (gss, s /p:40) Chrg 1 R: NL: 3.04E3 IDCUBE_Mexican Viagra_High_Tablet Elemental composition search Scrape_POS_1_ #106 on mass RT: 0.99 AV: 1 SB: , m/z= T: FTMS + p NSI Full ms m/z Theo. Mass Delta [ ] RDB Composition (ppm) equiv C22H31O4N6S m/z

17 Pai You Guo Herbal Slim Capsules Relative Abundance Clearly contains Sibutramine [M+H] + Elemental Comp. Simulation [M+H] + Capsule Powder Direct DART Spectrum [M+H] + Capsule Contents in ACN DART Spectrum m/z NL: 7.35E4 C 17 H 27 N Cl: C 17 H 27 N 1 Cl 1 p (gss, s /p:8) Chrg 1 R: NL: 1.68E5 ID-Cube_Pai You Elemental composition Guo_POWDER_POS_High_ #45 search on mass RT: AV: 7 SB: m/z= T: FTMS + p NSI Full ms m/z Theo. Mass Delta RDB Composition [ ] (ppm) equiv C 17H 27NCl C H O N NL: 1.72E6 ID-Cube_Pan You Guo_20 Elemental composition ug-ml_wet_pos_low_3_5 search on mass Capsules_ # RT: m/z= AV: 16 SB: T: m/z Theo. Mass Delta RDB Composition FTMS + (ppm) p NSI Full equiv. ms [ ] C 17H 27NCl C H O Cl

18 HCD Experiment -Pai You Guo Capsule Major Fragment Ion T: FTMS + p NSI Full ms @hcd20.00 [ ] HCD Gas on, 20 ev Relative Abundance Minor Fragment Ion Sibutramine [M+H] m/z

19 This image cannot currently be displayed. This image cannot currently be displayed. DART-SVP Ion Source Automated Sample Prep Simultaneous deposition of up to 96- liquid samples either from individual wells or rows of like samples

20 Mass Chromatograms for Standard Curve Development 96 samples in 20 minutes 10 X 200ppb to 50 ppm concentration

21 Post Run inspection of sample array

22 Mass Chromatograms 5ppm Standard Position 1 and 12 Blanks, Position 2-11 Sample Blanks in purple boxes

23 Standard Curve 200 o C, 2 mm/sec, 2 sec ionization (n = 10) 1.6E+06 Dimethoate Standard 1.4E+06 Ion Current 1.2E E E E+05 y = x R² = E E E Parts per million

24 Standard Curve -Acephate 200 o C, 2 mm/sec, 2 sec ionization (n = 10)

25 Standard Curve -Malathion 200 o C, 2 mm/sec, 2 sec ionization (n = 10) Shows poor correlation potentially due to large dynamic range in ion trap instrument

26 Cranberry/Apple juice spiked with three pesticides at two concentrations Mass Chromatograms for Protonated Pesticides indicate detection of 500 ppb Acephateand Dimethoatedirect from juice without prep Malathionnot detected at that level All detected at 5ppm CranAppleJuice Spiked with 5ppm Acephate

27 CranAppleJuice Spiked with 5ppm Acephate

28 CranAppleJuice Spiked with 5ppm Acephate

29 CranAppleJuice Spiked with 5ppm Acephate

30 Analysis of Various Juices Replicate analysis of Juices for three pesticides96 samples in Four commercial samples of juice analyzed for three pesticides Left to right in clusters of 12 Cranberry/Apple Grape Lemon Tomato Orange Low ppb or no pesticides detected in commercial product Cran/App Grape Lemon Tomato Orange

31 Analysis of Juices Inspection of dimethoate detection Expanded view of small mass range shows many sample related ions * Peak at mass corresponding to protonated dimethoate labelled ( * ) However no peak detected at C13 isotope mass ( ** ) therefore dimethoatenot detected **

32 Summary DART is gaining as a tool for non-specific screening of solid and liquid foodstuffs Pre-screening allows investigators to sample a larger number of samples increasing the probability of detection of adulterants and pesticides The ability to complete quantitative as well as qualitative analyses is enabled by better automation including use of our high throughput X-Z Transmission Module New technologies are evolving as customers make request for integration of automation with off-line sampling technologies such as liquid sample handlers

33 Screening for Carbendazim DART-SVP API 4000 QTRAP IonSense, Inc. 999 Broadway Suite 404 Saugus, MA USA March 25, 2012 Prepared By: E. Crawford

34 Carbendazim Unapproved Fungicide for Citrus Fruits in US 1. Objectives: Generate standard curvedata for carbendazimin orange juice ranging from 1 ppb 5000 ppb. Screen for carbendazimin a variety of orange juicesfrom the EU (2 countries) and the USA. European maximum residue limit * (MRL) is set at 200 ppbfor oranges and carbendazimis notapproved for use on citrus fruits in the US. The US FDA has set an action limitof 10 ppb. 2. Analysis Overview: No sample preparation liquid from consumer juice beverages directly analyzedby transmission mode direct analysis in real time (DART) source coupled with triple quad. Positive ion mode; carbendazim transition m/z * 34

35 Source parameters for DART-SVP DART-SVP Parameters 1. Heater: 250 C 2. He Flow: 2.5 L/min 3. Rail Speed: Constant 0.5 mm/s 35

36 DART-SVP Transmission Module Prep Workflow Stainless steel mesh screens sample surface allows liquid samples to be suspended on it 3 µl aliquot pipetted onto stainless steel mesh screen and allowed to completely dry before DART analysis 36

37 4.00E E+05 Carbendazim Standard Curve in Orange Juice 5.00E E+04 y = x R² = Average Peak Area (counts) 3.00E E E E E E E E E E E Concentration (ppb) n= 3 per conc. 37

38 25 Estimated Carbendazim Levels: Screening Juices 20 Measured Level ppb ND ND ND ND ND USA Apple USA Fruit Punch USA OJ 1 USA OJ 2 USA OJ 3 France OJ 1 Germany OJ 1 Fruit Juice Consumer Beverage Germany OJ 2 ND = Not Detected n= 8; except n = 16 for German OJs 38

39 Summary DART-SVP ion source coupled to the API 4000 QTRAP yielded LOD of 2 ppb and LOQ of 15 ppb. DART-SVP contact closure capability allows the user to acquire data directly through an Analyst sample queue. The standard curve generated for cardbendazim using the transmission DART method was very good with R 2 value across ppb. The reproducibility for an n = 3 across the concentration ranges was generally less than 18 % based on peak areas without internal standard correction. The two orange juices from Germany from the same manufacturer, but different lots yielded estimated carbendazim levels of 20 ppb, 10x below the Europe MRL, where the French orange juice contained only trace amounts. 39

40 Pesticide Screening US FDA utilizing Swabs Timothy Ng, Elizabeth Crawford, Brian Musselman IonSense, Inc. 999 Broadway Suite 404 Saugus, MA USA

41 Overview Thousands of samples imported daily Lessthan 0.2 percent inspected Official methods require extensive workup even with QuEChERs Hours of sample preparation 40 minute LC/MS Limit 8 samples per day per LC/MS New technology to protect Chinese customers and export industries

42 Method Development Thermo Exactive Settings DART- SVP Parameters 12-DIP-it Temp Profile to run 150, 250, 350 C Sample positioning in module: XX O XX O XX X = sample swab and O = blank swab wetted with solvent mixture Starting Temp: 150 C Temp increment: 100 C Sample speed: 0.5 mm/s Scan Parameters: Positive Ion Mode & Negative Ion Mode Resolution: Enhanced - 4 Hz Fragmentation: None, HCD Gas Off Scan Settings: 1 µ-scan by 250 ms max inject time AGC Target: Ultimate Mass Accuracy(5e 5 ) Exactive Inlet Parameters: Capillary Temp: 200 C Capillary Voltage: 25 V Tube Lens Voltage: 120 V Skimmer Voltage: 26 V

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44 Data

45 Temperature Profile of Swab - Major Ions Few Changes m/z

46 Temperature Profile of Swab Many Different Ions Detected at each temp

47 Mass Chromatogram for Strawberry Sample

48 At ultrahigh resolution hundreds of ions are detected. ToxIDprogram searches for accurate mass matches using a pesticide database. Many match mass values Mass Chromatograms used to determine relative abundance of different compound and their optimum temperature for desorption

49 Comparison of Theoretical isotope pattern (top) vs experimental result Lime Imazalil 350 C ppm Relative Abundance Theoretical Stable Isotope Pattern Experimental Result NL: 1.15E4 C 14 H 14 Cl 2 N 2 O +H: C 14 H 15 Cl 2 N 2 O 1 p (gss, s /p:40) Chrg 1 R: EPA Tolerance level 10 ppm NL: 4.99E6 Lime_MiniPolyesterSwab_150_25 0_350C POS_25K_ #6 56 RT: 2.22 AV: 1 SB: T: FTMS + p NSI Full ms [ ] m/z

50 Comparison of Theoretical isotope pattern (top) vs experimental result Relative Abundance Lime Carbendazim 350 C ppm Theoretical Stable Isotope Pattern NL: 2.09E4 C 9 H 9 N 3 O 2 +H: C 9 H 10 N 3 O 2 p (gss, s /p:40) Chrg 1 R: NL: 3.30E5 Lime_MiniPolyesterSwab_150_250_35 0C POS_25K_ # RT: AV: 18 SB: T: FTMS + p NSI Full ms [ ] m/z

51 Relative Abundance Lime Dodemorph 350 C ppm First and second isotope mass values match however third isotope is off by 37ppm and therefore not correct m/z or abundance NL: 1.91E4 C 18 H 35 NO +H: C 18 H 36 N 1 O 1 p (gss, s /p:40) Chrg 1 R: Not detected NL: 3.84E4 Lime_MiniPolyesterSwab_150_25 0_350C POS_25K_ #6 56 RT: 2.22 AV: 1 SB: T: FTMS + p NSI Full ms [ ] m/z

52 Confirm by matching Stable Isotope Patterns Two legal fungicides detected while other compounds not confirmed Pesticide screen completed in minutes