Ten Second DART -MS: Ultra-Fast QC Screening of Dietary Supplements and Pharmaceuticals with a Next Generation DART Source

Transcription

1 Ten Second DART -MS: Ultra-Fast QC Screening of Dietary Supplements and Pharmaceuticals with a Next Generation DART Source Elizabeth Crawford, Joseph LaPointe,and Brian Musselman IonSense, Inc. 999 Broadway Suite 44 Saugus, MA 196 USA 125 th AOAC Annual Meeting September 18-21, 211

2 Next Generation DART Source for Rapid Screening: DART-SVP vs. ID-CUBE Overview High Resolution/Accurate Mass measurements with benchtop Exactive Orbitrap mass spectrometer Dietary Supplement and Pharmaceutical Screening Are they safe and what s in them??? Why take a dietary supplement? Support immune system Reduce risk of illness/age related conditions To support healing process To boost energy and performancein activities Weight control Issue 52, August 26 September 8, 211

3 DART-SVP Ionization Source

4 DART-SVP Ion Source ID-CUBE: Built on DART Technology

5 ID-CUBE Ionization Source

6 MS Instrumentation Thermo Exactive Accurate mass measurements within 3 ppm for all work presented (external) Scan Parameters: Positive & Negative Ion Modes Resolution: High - 5, Fragmentation: None, HCD Gas Off Scan Settings: 1 µ-scan by 25 ms max inject time AGC Target: Balanced (1e 6 ) Exactive Inlet Parameters: Capillary Temp: 2 C 2 C Capillary Voltage: 25 V - 5 V Tube Lens Voltage: 12 V -12 V Skimmer Voltage: 26 V -25 V For all ID-CUBE experiments the Exactive Spray Voltagewas set to 4 kv and the following parameters set to zero: Sheath Gas Flow, Aux Gas Flow, Sweep Gas Flow

7 ID-CUBE Settings ID-CUBE Source: Desorption Temperature Settings: Low, Medium, High Sampling Time: Continuously heated for 1 seconds after initial signal observed

8 1. Spot 5 µl aliquot or powder residue on the OpenSpot Card 2. Let sample dry for easy transport or analyze directly when wet 3. Insert sample cardinto the slot on ID-CUBE Source 4. Set the desired heating setting on ID-CUBE control box and press button for instant desorption

9 DART-SVP vs. ID-CUBE Temperature Profiling

10 DART-SVP -Temp. Ramp Method 35⁰ C RT: ⁰ C RT:.71 RT: RT: ⁰ C RT: 5.37 RT: NL: 8.65E7 m/z= MS ICIS DART- SVP_Mesh_Sulfodene_ Skin_Med_Temp_Ramp _NEG_21181_2 Relative Abundance RT: Time (min) Run time of 5.5 min

11 ID-CUBE Temp. Gradient RT:.47 SN: 16RMS 1 Relative Abundance LOW MEDIUM HIGH RT:.42 SN: 671RMS RT:.15 SN: 31RMS Time (min).6 NL: 1.29E5 m/z= MS ICIS IDCUBE_Alli Capsule Extract #1_TEMP_RAMP_WET _POS_ Run time of.65 min LOW NL: 3.97E5 IDCUBE_Alli Capsule Extract #1_TEMP_RAMP_WET_POS_211819#13-18 RT: AV: 6 T: FTMS + p NSI Full ms [1.-2.] Relative Abundance MEDIUM HIGH m/z NL: 3.8E5 IDCUBE_Alli Capsule Extract #1_TEMP_RAMP_WET_POS_211819#28-36 RT: AV: 9 SB: T: FTMS + p NSI Full ms [1.-2.] NL: 1.45E5 IDCUBE_Alli Capsule Extract #1_TEMP_RAMP_WET_POS_211819#41-51 RT: AV: 11 SB: T: FTMS + p NSI Full ms [1.-2.]

12 Omega Fatty Acid & Herbal Supplements

13 Nature Made Triple Omega Capsules 2% Dilution in Toluene T: FTMS - p NSI Full ms [1.-1.] Eicosapentaenoic Acid (EPA) Omega-3 Omega-6 Omega-9 7 Relative Abundance Docosahexaenoic Acid (DHA) m/z

14 Nature Made Triple Omega 2% Dilution in Toluene T: FTMS - p NSI Full ms [1.-1.] 1 9 Oleic Acid C 18 H 33 O 2 Omega-3 Omega-6 Omega α Linolenic Acid Linoleic Acid Relative Abundance Stearidonic Acid C 18 H 29 O C 18 H 31 O C 18 H 35 O C 18 H 27 O m/z

15 Omega-3, -6, -9 Fatty Acids in Dietary Oil Supplements Stearidonic Acid α-linolenic Acid Linoleic Acid Oleic Acid n=5 per dietary oil brand Normalized Average Peak Area Nature Made Triple Omega Nature Made Flax Oil Target Origin Flax Oil

16 Replicate Samples Triple Omega RT: RT: 1.69 RT:.21 SN: 1432RMS 1 SN: 131RMS RT: 1.2 RT: 2.3 SN: SN: 11RMS Omega-3 Omega-6 Omega-9 RT: 2.93 SN: 1246RMS 3. NL: 3.49E5 m/z= MS ID-Cube_Nature Made Triple Oemga_Cap Oleic Acid 1_High_WET_NEG_1_Different Card_ Relative Abundance RT:.21 RT: 1.69 SN: 26RMS SN: RT: 2.93 RT: RMS RT: 2.3 SN: 182RMS.25 SN: 1562RMS 1.71 SN: 1465RMS RT:.21 SN: 1281RMS.25 RT: 1.67 SN: 1284RMS RT: 1.2 SN: 91RMS RT: 2.3 SN: 932RMS RT: 2.93 SN: 1169RMS 3. NL: 1.99E5 m/z= MS ID-Cube_Nature Made Triple Oemga_Cap Linoleic Acid 1_High_WET_NEG_1_Different Card_ NL: 1.93E5 m/z= MS ID-Cube_Nature Made Triple αoemga_cap Linolenic Acid 1_High_WET_NEG_1_Different Card_ RT:.21 RT: 1.67 SN: 635RMS SN: 657RMS RT: 2.93 RT: 1.2 RT: 2.3 SN: 563RMS.25 SN: 414RMS 1.71 SN: 431RMS Time (min) NL: 6.28E4 m/z= MS ID-Cube_Nature Made Triple Stearidonic Oemga_Cap 1_High_WET_NEG_1_Different Card_ Acid

17 Omega-3 and -9 Fatty Acids Found in Marine Oil Samples EPA DHA Oleic Acid Normalized Average Peak Area Nature Made Fish Oil Nature's Bounty Fish Oil n=5 per dietary oil brand Nature Made Triple Omega Schiff's Megared Krill Oil

18 .4.35 QC Monitoring of Anthocyanins in an Elderberry Herbal Supplement Delphinidin Pelargonidin Cyanidin 8.6 % CV 27.2 % CV 11.9 % CV Normalized Average Peak Area Elderberry Capsules (n=3 per Capsule)

19 Performance Drug

20 Scraping Contents of Mexican sildenafil This tablet was sold by a drug pusher in Mexico as a male enhancement drug that resembles the appearance of the well known erectile dysfunction (ED) drug, Viagra. The tablet was sliced open and using a standard glass capillary tube a small amount of material was scarped from the inside of the tablet, transferred onto the mesh and directly screened for the FDA regulated API sildenafil.

21 Mexican sildenafil Tablet Scraping Relative Abundance [M+H] + DART Spectrum [M+H] + Elemental Composition Simulation NL: 1.7E4 C 22 H 31 O 4 N 6 S: C 22 H 31 O 4 N 6 S 1 p (gss, s /p:4) Chrg 1 R: NL: 3.4E3 IDCUBE_Mexican Viagra_High_Tablet Elemental composition search Scrape_POS_1_211822#16 on mass RT:.99 AV: 1 SB: , m/z= T: FTMS + p NSI Full ms m/z Theo. Mass Delta [1.-1.] RDB Composition (ppm) equiv C 22 H 31 O 4 N 6 S m/z

22 Weight Loss Aid/Supplements

23 Pai You Guo Herbal Slim Capsules Article cut from Boston Globe newspaper, August 16, 211. November 12, 29 After pressure from FDA GMP Herbal Products, Inc. issued a voluntary nationwide recall for Pai You Guo, which was found to contain undeclared drug ingredients.

24 Pai You Guo Slim Capsules Listed Ingredients on package insert: Evil powder of, low gather the sugar, malt, chitin, the, meal fiber, apple withdraws the thing, Living the plain C, vitamin E, vitamin B2

25 Pai You Guo Tablet Direct Powder The capsules were twisted open and a very small amount of the fine powder was transferred with a glass capillary tube onto the mesh sampling area of an OpenSpot card for rapid screening for the FDA regulated API sibutramine. The capsule powder was also dissolved in ACN and directly analyzed from the sample cards.

26 Pai You Guo Herbal Slim Capsules Relative Abundance Clearly contains Sibutramine [M+H] + Elemental Comp. Simulation [M+H] + Capsule Powder Direct DART Spectrum [M+H] + Capsule Contents in ACN DART Spectrum m/z NL: 7.35E4 C 17 H 27 N Cl: C 17 H 27 N 1 Cl 1 p (gss, s /p:8) Chrg 1 R: 5 NL: 1.68E5 ID-Cube_Pai You Elemental composition Guo_POWDER_POS_High_211821#45 search on mass RT: AV: 7 SB: m/z= T: FTMS + p NSI Full ms m/z Theo. Mass Delta RDB Composition [1.-1.] (ppm) equiv C 17 H 27 N Cl C H O N NL: 1.72E6 ID-Cube_Pan You Guo_2 Elemental composition ug-ml_wet_pos_low_3_5 search on mass Capsules_211821# RT: m/z= AV: 16 SB: T: m/z Theo. Mass Delta RDB Composition FTMS + (ppm) p NSI Full equiv. ms [1.-1.] C 17 H 27 N Cl C H O Cl

27 HCD Experiment -Pai You Guo Capsule Major Fragment Ion T: FTMS + p NSI Full ms2 1.@hcd2. [1.-1.] HCD Gas on, 2 ev 9 Relative Abundance Minor Fragment Ion Sibutramine [M+H] m/z

28 FDA Warning Jan. 18, 21 Counterfeit Alli The 12 capsule Alli weight loss aid refill pack, which was subject of an FDA warning in Jan. 21 was purchased from an online vendor. Upon receipt the product appeared to be authentic based on the appearance of the packaging and capsule contents.

29 Orlistat Detected Elemental composition search on mass m/z= m/z Theo. Mass Delta (ppm) RDB equiv C 29 H 54 O 5 N C H O Orlistat [M+H] Composition Elemental composition search on mass m/z= m/z Theo. Mass Delta (ppm) RDB equiv C 29 H 57 O 5 N C H O Orlistat [M+NH 4 ] + Composition Relative Abundance m/z

30 Conclusions ID-CUBEon the Exactive Orbitrap platform provides extremely fast accurate mass measurements (< 3 ppm with external calibration) to complete rapid screening in 1 seconds or less per sample for liquids and powders Optimizationof the desorption settings with the ID-CUBE is 8x faster than with the DART-SVP source since sample heating and cooling is nearly instantaneous The listed Omega-3, -6, -9 fatty acids in several oil supplements were easily identified and relative levels per capsule were compared Mexican sildenafil tablet purchased on the street did indeed contain sildenafil, however dosage level is unknown Sibutraminewas clearly detected in the banned Pai You Guo herbal slim capsule product from both the powder directly and from solution The Alliweight loss aid capsules purchased from an online vendor appeared to be legitimate and did not show any signs of gross contamination or adulteration Acknowledgements The authors would like to kindly thank Maciej Bromirski at Thermo Fisher Scientific for coordinating the collaborative efforts for the loan of an Exactive Orbitrap Mass Spectrometer.