Chromatography. NUCLEOSHELL Bluebird RP 18 MACHEREY-NAGEL. Fast analyses under highly aqueous conditions
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1 MACHEREY-NAGEL NUCLEOSHELL Bluebird RP 18 Chromatography Fast analyses under highly aqueous conditions nspecial octadecyl core-shell phase with hydrophilic endcapping nsuitable for LC/MS due to low bleeding characteristics nextremely durable in 100 % aqueous mobile phase
2 Key features n Special octadecyl core-shell phase with hydrophilic endcapping n Distinct polar selectivity n Extremely durable in 100 % aqueous mobile phase n Excellent base deactivation n Suitable for LC/MS due to low bleeding characteristics Recommended Applications n Water-soluble vitamins n Organic acids n Nitrosamines n Pesticides n Sweeteners n Pharmaceuticals n Very polar analytes USP L1 Similar phases: Kinetex Polar C18 Technical data: Octadecyl modification with polar endcapping on core-shell particles ph stability: 1 8 Particle size: 2.7 µm (core 1.7 µm) Pore size: 90 Å Specific surface: 130 m 2 /g Carbon content: % NUCLEOSHELL Bluebird RP 18 OH TMS OH Batch-to-batch reproducibility MN Appl. No Column: EC 0/4 NUCLEOSHELL Bluebird RP 18, 2.7 μm MN REF: Eluent: 2 mm ammonium dihydrogenphosphate solution methanol (3:6, v/v), ph = 7.0 Flow rate: 1.0 ml/min Temperature: 40 C Detection: UV, 24 nm Injection: μl Uracil 4 µg/ml Ethyl benzoate 181 µg/ml Lidocaine 1134 µg/ml Naphthalene 1134 µg/ml Biphenyl 4 µg/ml Acenaphthene 227 µg/ml The mixture was diluted to 4 ml with water Excellent reproducibility High batch-to-batch reproducibility of NUCLEOSHELL Bluebird RP 18 columns shows reliable results for different LOTs. Peak No. Uracil 1 Ethyl benzoate 2 Lidocaine 3 Naphthalene 4 Biphenyl Acenaphthene 6 Absorbance [mau] batch batch batch min 2
3 Organic acids MN Appl. No Column: EC 10/4 NUCLEOSHELL Bluebird RP 18, 2.7 μm MN REF: Eluent: 0 mm potassium dihydrogenphosphate solution, ph = 2. Flow rate: 2.0 ml/min Temperature: 40 C Peak No. Tartaric acid 1 Malic acid 2 Shikimic acid 3 Lactic acid 4 Acetic acid Detection: Injection: Tartaric acid Malic acid Shikimic acid Lactic acid Acetic acid Citric acid Fumaric acid Acrylic acid Arbutin UV, 210 nm 3 μl 13 µg/ml 2162 µg/ml 27 µg/ml 2703 µg/ml 2703 µg/ml 1081 µg/ml 41 µg/ml 676 µg/ml 216 µg/ml Citric acid 6 Fumaric acid 7 Acrylic acid 8 Arbutin 9 Chromatogram Absorbance [mau] Enhanced stability Excellent performance using 100 % aqueous mobile phase min Polar and non polar substances MN Appl. No Column: EC 100/2 NUCLEOSHELL Bluebird RP 18, 2.7 μm 100/2.1 Kinetex 2.6 µm Polar C18, 100 Å MN REF: Eluent: 2 mm ammonium dihydrogenphosphate solution methanol (3:6, v/v), ph = 7.0 Flow rate: 0.66 ml/min Temperature: 40 C Detection: UV, 24 nm Injection: μl Uracil 4 µg/ml 2,7-DHN 4 µg/ml 2,3-DHN 4 µg/ml Ethyl benzoate 180 µg/ml Lidocaine 1123 µg/ml Naphthalene 1123 µg/ml Biphenyl 4 µg/ml Acenaphthene 22 µg/ml DHN = Dihydroxynaphthalene Superior to Kinetex Polar C18 NUCLEOSHELL Bluebird RP 18 shows a complete separation of lidocaine, naphthalene and additionally better peak shapes for all analytes compared to Kinetex Polar C18. Peak No. Uracil 1 2,7-Dihydroxynaphthalene 2 2,3-Dihydroxynaphthalene 3 Ethyl benzoate 4 Lidocaine Naphthalene 6 Biphenyl 7 Acenaphthene 8 Arbutin 9 Absorbance [mau] min 3
4 Pesticides MN Appl. No Column: EC 0/4.6 NUCLEOSHELL Bluebird RP 18, 2.7 μm MN REF: Eluent A: 0.1 % formic acid in water Eluent B: 0.1 % formic acid in methanol Gradient: in min from % to 100 % B, hold for 1.0 min, in 0.1 min to % B, hold % B for 3.9 min Flow rate: 0.7 ml/min Temperature: 30 C Detection: MS, SMRM Injection: 20 μl 2 ng/ml for each analyte in water acetonitrile (4:1, v/v) Sample pretreatment according to MN Appl. No Dispersive solid phase extraction (EN 1662) QuEChERS Mix I (MN REF ) and QuEChERS Mix III (MN REF ) Sample matrix: red grape Chromatogram.e6.0e6 4.e6 4.0e6 3.e6 3.0e6 2.e6 2.0e6 1.e6.0e min RT [min] RT [min] Cyromazine 0.9 Omethoate 1.93 Propamocarb 1.30 Dinotefuran 2.09 Formetanate HCl 1.36 Mexacarbate 2.13 Aminocarb 1.40 Thiabendazole 2.19 Methamidophos 1.43 Aldicarb sulfone 2.20 Pymetrozine 1.2 Fuberidazole 2.29 Acephate 1.69 Oxamyl 2.32 Carbendazim 1.84 Flonicamid 2.41 Nitenpyram 1.84 Methomyl 2.44 Continued on page 4
5 Continued from page 4 RT [min] Thiamethoxam 2.6 Ethirimol 2.60 Monocrotophos 2.67 Dicrotophos 2.80 Pirimicarb 2.88 Imidacloprid 2.94 Clothianidin 2.96 Fenuron 3.03 Vamidothion Hydroxycarbofuran 3.10 Dioxacarb 3.10 Dimethoate 3.17 Acetamiprid 3.18 Imazalil 3.22 Cymoxanil 3.28 Simetryn 3.29 Prometon 3.37 Secbumeton 3.37 Terbumeton 3.39 Thiacloprid 3.43 Mevinphos (Mix of Isomers) 3.46 Butocarboxim 3.1 Spiroxamine (Mix of Isomers) 3.2 Aldicarb 3. Fenpropimorph 3. Tricyclazole 3. Oxadixyl 3.60 Carbetamide 3.71 Mesotrione 3.71 Ametryn 3.80 Methoprotryne 3.80 Propoxur 3.84 Metribuzin 3.8 Bendiocarb 3.89 Carbofuran 3.89 Thidiazuron 3.92 Tebuthiuron 3.94 Pyracarbolid 3.98 Prometryn 4.03 Terbutryn 4.04 Carbaryl 4.0 Carboxin 4.0 Monolinuron 4.10 Ethiofencarb 4.13 Fluometuron 4.17 Pyrimethanil 4.19 RT [min] Chlorotoluron 4.20 Flutriafol 4.20 Spinosad (Spinosyn A) 4.20 Isoprocarb 4.24 Propham 4.24 Butoxycarboxim 4.28 Methabenzthiazuron 4.28 Metalaxyl 4.29 Isoproturon 4.31 Spinosad (Spinosyn D) 4.32 Isocarbophos 4.33 Spinetoram 4.33 Cycluron 4.34 Hydramethylnon 4.34 Forchlorfenuron 4.3 Chlorantraniliprole 4.41 Bupirimate 4.43 Diethofencarb 4.47 Emamectin B1a benzoate 4.48 Ethiprole 4.48 Metobromuron 4.0 Fenobucarb 4.1 Fenhexamid 4.2 Furalaxyl 4.2 Ethofumesate 4.3 Halofenozide 4.6 Azoxystrobin 4.7 Fenamidone 4.7 Linuron 4.8 Methiocarb 4.9 Carfentrazone-ethyl 4.60 Diuron 4.60 Paclobutrazol 4.60 Siduron 4.60 Promecarb 4.62 Dimethomorph (Mix of Isomers) 4.63 Fludioxinil 4.63 Cyprodinil 4.64 Flutolanil 4.64 Myclobutanil 4.64 Triadimenol 4.64 Triadimefon 4.6 Acibenzolar-S-methyl 4.69 Cyproconazole (Mix of Isomers) 4.69 Bifenazate 4.70 Bromuconazole (Mix of Isomers) 4.70 Continued on page 6
6 Continued from page RT [min] Clethodim (Mix of Isomers) 4.70 Mepronil 4.70 Triticonazole 4.72 Methoxyfenozide 4.73 Chloroxuron 4.74 Mefenacet 4.74 Tetraconazole 4.74 Butafenacil 4.7 Flufenacet 4.76 Spirotetramat 4.76 Iprovalicarb (Mix of Isomers) 4.77 Etaconazole (Mix of Isomers) 4.78 Fipronil 4.78 Fluoxastrobin 4.80 Epoxiconazole 4.82 Fenbuconazole 4.82 Flusilazole 4.84 Mepanipyrim 4.84 Cyazofamid 4.8 Difl ubenzuron 4.8 Prochloraz 4.87 Fenoxycarb 4.91 Neburon 4.93 Dimoxystrobin 4.94 Penconazole 4.94 Rotenone 4.9 Tebuconazole 4.9 Picoxystrobin 4.97 Flubendiamide 4.98 Propiconazole (Mix of Isomers) 4.99 Hexaconazole.00 Metconazole.00 RT [min] Ipconazole (Mix of Isomers).17 Trifl oxystrobin.20 Clethodim (Mix of Isomers).23 Metafl umizone.26 Tebufenpyrad.29 Furathiocarb.30 Tebufenozide.36 Piperonyl butoxide.38 Temephos.39 Flufenoxuron.41 Hexythiazox.41 Fenazaquin.42 Pyriproxyfen.42 Quinoxyfen.42 Propargite.4 Etoxazole.46 Spirodiclofen.2 Eprinomectin.4 Avermectin B1a.7 Fenpyroximate.8 Mandipropamid.8 Pyridaben.60 Doramectin.66 Nuarimol.66 Moxidectin.71 Ivermectin.74 Difenoconazole (Mix of Isomers).80 Trichlorfon 6.18 Fast analyses LC/MS analysis of more than 190 pesticides in less than 6 minutes on NUCLEOSHELL Bluebird RP 18. Chromatogram on page 4 Amitraz.01 Benalaxyl.03 Trifl umizole.03 Trifl umuron.0 Diniconazole.09 (Monceren) Pencycuron.10 Diclobutrazol.10 Etaconazole (Mix of Isomers).10 Pyraclostrobin.10 Indoxacarb.13 Thiobencarb.13 Benzoximate.16 Clofentezine.16 Buprofezin.17 6
7 Water-soluble vitamins MN Appl. No Column: EC 0/4.6 NUCLEOSHELL Bluebird RP 18, 2.7 μm MN REF: Eluent A: 10 mm ammonium formate % formic acid in water Eluent B: 10 mm ammonium formate % formic acid in methanol Gradient: 9 % B in 4. min, hold for 1.0 min, back to % B in 0.1 min, hold for 4.4 min Flow rate: 1.3 ml/min Temperature: 40 C Detection: MS, SMRM Injection: μl 000 ng/ml for ascorbic acid, 7 ng/ml for all other analytes RT [min] [M+H] + Q 1 (Quantifier) Q 2 (Qualifier) Thiamine Ascorbic acid Pyridoxal Pyridoxine Nicotinic acid Nicotinamide Pantothenic acid Folic acid Biotin Cyanocobalamin Ribofl avin Multivitamin tablet, 100 mg/ml 8.0e 6.0e 4.0e 10 times diluted 8.0e 6.0e 4.0e 100 times diluted 1.4e6 6.0e Ascorbic acid Folic acid Biotin 1.0e4 7.e3.0e3 2.e min min Pyridoxal Nicotinic acid Ribofl avin 6.0e6.0e6 4.0e6 3.0e6 2.0e min min Pyridoxine Pantothenic acid 1.0e7 8.0e6 6.0e6 4.0e6 2.0e min min Thiamine Nicotinamide Cyanocobalamine 7
8 THC and its metabolites MN Appl. No Column: EC 0/4.6 NUCLEOSHELL Bluebird RP 18, 2.7 μm MN REF: Eluent A: 0.1 % formic acid in water Eluent B: 0.1 % formic acid in acetonitrile Gradient: in 2. min from 0 % to 90 % B, hold for 0. min, in 0.1 min to 0 % B, hold 0 % B for 2.9 min Flow rate: 1.3 ml/min Temperature: 40 C Detection: MS, SMRM Injection: μl 0 ng/ml for each analyte RT [min] [M+H] + Q 1 (Quantifier) Q 2 (Qualifier) THC-OH THC-COOH THC THC-OH 1.e 1.0e.0e4 THC-COOH.0e 4.0e 3.0e 1.0e THC 6.0e4 4.0e4 Q 1 (Quantifi er) Q 2 (Qualifi er) min min min Sulfa drugs MN Appl. No Column: EC 0/4.6 NUCLEOSHELL Bluebird RP 18, 2.7 μm MN REF: Eluent A: 0.1 % formic acid in water Eluent B: 0.1 % formic acid in methanol Gradient: in 4.0 min from % to 20 % B, in 1.0 min to 80 % B, hold 80 % B for 0. min, in 0.1 min to % B, hold % B for 4.4 min Flow rate: 1.3 ml/min Temperature: 0 C Detection: MS, MRM Injection: μl 100 ng/ml for each analyte RT [min] [M+H] + Q 1 (Quantifier) Q 2 (Qualifier) Sulfachlorpyridazine Sulfamethoxazole Sulfadoxine Sulfadimethoxine Sulfaquinoxaline Chromatogram 4.0e6 3.0e6 RT [min] [M+H] + Q 1 (Quantifier) Q 2 (Qualifier) Sulfacetamide Sulfadiazine Sulfapyridine Sulfatiazole Sulfamerazine Sulfadimidine Sulfamethoxypyridazine Sulfamonomethoxine e min 8
9 Sweeteners MN Appl. No Column: EC 100/2 NUCLEOSHELL Bluebird RP 18, 2.7 μm MN REF: Eluent A: 0.1 % formic acid in water Eluent B: 0.1 % formic acid in methanol Gradient: 9 % B in 4. min, hold for 1.0 min, back to % B in 0.1 min, hold for 4.4 min Flow rate: 0.3 ml/min Temperature: 40 C Detection: MS, SMRM Injection: 10 μl 1000 ng/ml for sucralose, 100 ng/ml for all other analytes Polarity RT [min] [M-H] Q 1 (Quantifier) Q 2 (Qualifier) Acesulfame negative Aspartame negative Cyclamate negative Sucralose negative Saccharin negative NHDC negative Polarity RT [min] [M+H] + Q 1 (Quantifier) Q 2 (Qualifier) Advantame positive Neotame positive Acesulfame, 100 ng/ml Aspartame, 100 ng/ml.0e4 4.0e4 3.0e4 1.0e min min Cyclamate, 100 ng/ml Sucralose, 1000 ng/ml 1.4e 1.0e.0e min min Saccharin, 100 ng/ml NHDC 100 ng/ml 3.0e4 1.e4 1.0e e min min Advantame, 100 ng/ml Neotame 100 ng/ml.0e 1.e 4.0e 1.0e.0e4 3.0e 1.0e min min 9
10 Radiocontrast agents MN Appl. No Column: EC 100/2 NUCLEOSHELL Bluebird RP 18, 2.7 μm MN REF: Eluent A: mmol/l ammonium formate + 0. % formic acid % acetonitrile in water Eluent B: acetonitrile Gradient: hold % B for 1 min, in 4 min from % to 80 % B, hold for 1.0 min, in 0.1 min to % B, hold % B for 4.9 min Flow rate: 0.4 ml/min Temperature: 30 C Detection: MS, MRM Injection: 20 μl ng/ml for each analyte MRM-Overlay 6.0e4 RT [min] [M+H] + Q 1 (Quantifier) Q 2 (Qualifier) Iohexol 0.7/ Iopromide 1.93/ Iopamidol Iomeprol Diatrizoic acid Iopamidol 6.0e4 4.0e4 4.0e4 Iohexol min min Iomeprol 4.0e e4 1.0e4 Iopromide min min Diatrizoic acid min min 10
11 Drug analytes MN Appl. No Column: EC 0/4.6 NUCLEOSHELL Bluebird RP 18, 2.7 μm MN REF: Eluent A: 0.1 % formic acid in water Eluent B: 0.1 % formic acid in methanol Gradient: in 4. min from % to 90 % B, hold for 0. min, in 0. min to % B, hold 0 % B for 4. min Flow rate: 1.3 ml/min Temperature: 30 C Detection: MS, SMRM Injection: μl 0 ng/ml for each analyte RT [min] [M+H] + Q 1 (Quantifier) Q 2 (Qualifier) Amphetamine ,4-Methylenedioxyamphetamine Isoxsuprine Fentanyl Sibutramine Fenbutrazate RT [min] [M-H] Q 1 (Quantifier) Q 2 (Qualifier) Hydrochlorothiazide Furosemide e6 Fenbutrazate 3.0e Furosemide 4.0e6 3.0e6 Amphetamine 3,4-Methylenedioxyamphetamine Isoxsuprine Sibutramine Hydrochlorothiazide 2.0e6 1.0e Fentanyl min min 11
12 Ordering information Length 0 mm 7 mm 100 mm 12 mm 10 mm NUCLEOSHELL Bluebird RP 18, 2.7 µm EC columns (pack of 1) 2 mm ID mm ID mm ID mm ID Selection of guard columns For EC column with ID of REF guard column Required guard column holder (Column proctection system) 2 mm EC 4/2 (pack of 3) / 4 / 4.6 mm EC 4/3 (pack of 3) Registered trademarks Kinetex Phenomenex (USA) NUCLEOSHELL MACHEREY-NAGEL GmbH & Co. KG (Germany) New Dawn Photography Serhyi Shullye Radmanovac Marius Graf Jonas Glaubitz fotolia.com KATEN NUCLEOSHELL Bluebird en2 / 2 / 0 / DD Printed in Germany MACHEREY-NAGEL GmbH & Co. KG Neumann-Neander-Str Düren Germany
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