A New Dimension in Preparative HPLC

Transcription

1 New Dimension in Preparative HPL... we Meet your Needs

2 NULEODUR HTec 6 - key features: reliable and durable standard RP phase for up-scaling to preparative scale, suited for L/MS high loadability and excellent stability outstanding base deactivation technical characteristics: high density octadecyl modification ( ) pore size Å, particle sizes μm, μm and μm for analytical and preparative separations, carbon content %, ph stability -, high reproducibility from batch to batch Engelhardt-Test excellent symmetry and peak shape over the entire polarity range olumn: NULEODUR - HTec, x mm Eluent: methanol / water 9: (w/w) Flow rate:. ml/ Temperature: Inj. volume: μl Pressure: bar Detection: UV, nm Tf =. Tf =.. uracil. anilin. phenol. p-ethyl aniline. N,N-dimethyl aniline 6. toluene. ethyl benzene 6 α /6 = MN ppl. No. MS bleeding test low bleeding, good MS efficiency olumns: NULEODUR - HTec, Waters Xterra, μm ( x.6 mm each) HPL conditions: Eluent : water Eluent : acetonitrile Gradient: Start: % % in % % % in Flow rate:.6 ml/ Temperature: MS conditions: sample temperature: Needle: kv one voltage: + V Detection: ESI+ recommended application: sophisticated analytical and preparative separations of basic, neutral and acidic pharmaceuticals, derivatised ao acids, pesticides, fat-soluble vitas, aldehydes, ketones and phenolic compounds USP L Tanaka diagram outstanding base deactivation and hydrophobic selectivity ion exchange capacity ph. (x ) NULEODUR HTec capacity (x.)..... hydrophobicity (x ) counts NULEODUR - HTec Waters Xterra MS, μm Xterra ion exchange capacity ph.6 (x ) steric selectivity (x ) HTec hydrogen bonding capacity (x ) Retention Time [] MN

3 ase Deactivation NULEODUR HTec: excellent base deactivation Phase comparison ase deactivation test olumns: x.6 mm each; particle size μm Eluent: methanol / mm KH PO, ph (:, v/v) Flow rate:. ml/ Temperature: Detection: UV, nm.,-dihydroxynaphthalene (metal ion content).,-dihydroxynaphthalene (metal ion content). toluene (hydrophobicity, H group selectivity). ethylbenzene (hydrophobicity, H group selectivity). acenaphthene (silanol activity) 6. amitriptyline (silanol activity). o-terphenyl (steric selectivity). triphenylene (steric selectivity) Tf 6/ =. 6 NULEODUR HTec olumns: NULEODUR - HTec ( x.6 mm) Waters Xterra MS, μm ( x.6 mm) Spherisorb - ODS- ( x.6 mm) Eluent: acetonitrile / water (:, v/v) Flow rate:. ml/ Temperature: Detection: UV, nm Important note: critical test without buffer, acetonitrile instead of methanol (no masking of pyridine by MeOH protons) Quality criteria: pyridine elutes prior to phenol, maximal resolution, tailing (Tf) about mu pyridine: Tf =. NULEODUR HTec. 6 Tf 6/ =. NULEODUR Gravity. -. phenol Tf 6/ =. NULEODUR Isis... mu Waters Xterra MS pyridine: Tf =. 6 Tf 6/ =. NULEODUR Pyramid.. phenol / NULEODUR Sphinx RP. 6. mu pyridine: Tf =. Spherisorb - ODS- 6 mu 6 Tf 6/ =. 6 NULEODUR ec.. -. phenol ,,,,,,, Waters Xterra MS MN ppl. No. more applications at /apps MN

4 ph Stability & Transferability NULEODUR HTec excellent transferability from analytical to preparative particle sizes good peak symmetry and imal peak broadening available in, and μm ph stability test High ph-values can destroy the silica support resulting in dead volume and loss of theoretical plates. olumn: NULEODUR - HTec, x mm Eluent: methanol / mm triethylae, ph (:) Flow rate: ml/ Temperature: Detection: UV, nm Inj. volume:. μl Injections: Peak: theophylline NULEODUR HTec % Initial N Transferability olumn: NULEODUR - HTec x.6 mm each Eluent: methanol / mm KH PO, ph (:) Flow rate:. ml/ Temperature: Detection: UV, nm 6 Tf 6/ =. μm 6 % Initial Retention 6 ph / 9 9 hours in mm TE, ph, ph stability test Low ph-values can attack the -modification resulting in loss of retention. olumn: NULEODUR - HTec, x mm Eluent: acetonitrile / % TF in water, ph (:, v/v) Flow rate: ml/ Temperature: Detection: UV, nm Inj. volume:. μl Injections: 69. pyridine. toluol. ethylbenzene ph / 6 hours in N / % TF in water, ph at NULEODUR HTec - mu 6,,,,,,.,-dihydroxynaphthalene (metal ion content).,-dihydroxynaphthalene (metal ion content). toluene (hydrophobicity, H group selectivity). ethylbenzene (hydrophobicity, H group selectivity). acenaphthene (silanol activity) 6. amitriptyline (silanol activity). o-terphenyl (steric selectivity). triphenylene (steric selectivity) 6 Tf 6/ =.9 Tf 6/ =.9 μm μm MN

5 pplications nalytical application examples for NULEODUR HTec Sunscreen ingredients olumn: NULEODUR - HTec, x mm Eluent: methanol / mm ammonium acetate, ph. (:, v/v) Flow rate:.9 ml/ Temperature: Inj. volume: μl Detection: UV, nm mu. benzophenone. methylbenzylidene camphor. Uvinul Plus. octocrylene. ethylhexyl dimethyl P 6 6. ethylhexyl methoxycinnamate. ethylhexl salicylate 6 ntibiotics olumn: NULEODUR - HTec, x mm Eluents: : acetonitrile :. % TF Gradient: % for % 6 % in. 6 % for. 6 % % in %. % in Flow rate:.9 ml/ Temperature: Inj. volume: μl Detection: UV, nm oncentration: μg/ml, 6,,, mu. amoxicillin. enrofloxacin. cinoxacin. oxolinic acid. nalidixic acid 6. penicillin V. cloxacillin 6 -,,,,,,,,,,, -,,,,,,, 6,,, 9, MN ppl. No. 6 MN ppl. No. 6 Steroids olumn: NULEODUR - HTec, x mm Eluents : acetonitrile : water Gradient: % 6 % in 6 % for 6 % % in % for Flow rate:. ml/ Temperature: Inj. volume: μl Detection: UV, nm 6 - mu. estriol. prednisolone. cortisone. estradiol. testosterone 6. estrone. 6α-methyl-β-hydroxyprogesterone. 6α-methyl-β-hydroxyprogesterone 9. progesterone 9,,, 6,,,,, 6,,, 6 MN ppl. No. Sweeteners olumn: NULEODUR - HTec, x.6 mm Eluents: : acetonitrile : mm KH PO, ph. Gradient: % for. % % in 9. % for Flow rate:. ml/ Temperature: Inj. volume: μl Detection: UV, nm. acesulfame oncentration:. mg/ml each. saccharin 6. aspartame mu. benzoic acid. sorbic acid 6. dehydroacetic acid -,,,,,,, more applications at /apps 6 MN ppl. No. MN

6 Up-Scaling NULEODUR HTec... ideal for up-scaling.. Identical peak shapes and retention while up-scaling from analytical to preparative column dimension. dp up (mm) ml / da ua (,6 mm), ml / = =... E x.6 mm NULEODUR - HTec VP x mm NULEODUR - HTec olumns: E x.6 mm NULEODUR - HTec VP x mm NULEODUR - HTec Eluent: acetonitrile / water (:, v/v) Flow rate:. ml/. ml/ Temperature: Detection: UV, nm Pressure: bar 9 bar. phenol. naphthalene. anthracene ( mg/ml each) Inj. volume: μl 6 μl MN ppl. No. Scale up factors and parameters for common MN column dimensions ID x length [mm] x x x 6 x x x x x x Linear scale-up factor 6, 6,6 6 6, Typical sample mass* [mg],,,,,6 6, Typical flow rate [ml/],, * For RP material; the maximum amounts given here always depend on the separation problem and on the sample composition. In some cases half of the amount given can cause drastic overload, in other cases the maximum amounts can be even higher still giving acceptable separations. 6 MN

7 olumn Life Time NULEODUR HTec... exceptional column life time under critical conditions olumn: VP x mm NULEODUR - HTec Eluent: acetonitrile / formic acid in water, ph. (:9, v/v) Flow rate: ml/ Temperature: Detection: UV, nm Pressure: bar Inj. volume: μl Injections: 6 over Injections approx. Injections. xanthine (6. mg/ml). theobroe (. mg/ml) approx. Injections. theophylline (. mg/ml). caffeine (. mg/ml) approx. Injections approx. Injections approx. Injections Sample dissolved in DMSO. NULEODUR HTec shows reproducible retention times and excellent peak shapes even after injections. MN ppl. No. 9 at high flow rates first injection Overlay first and & rd injection olumn: VP x mm NULEODUR - HTec Eluent: acetonitrile / water (:, v/v) Flow rate: 6 ml/ Temperature: Detection: UV, nm Pressure: bar Inj. volume: μl. acetophenone. propanone. butyrophenone. valerophenone 6 rd injection NULEODUR HTec shows reproducible retention times and excellent peak shapes even during operations at very high flow rates. -,,, 6,,, MN ppl. No. MN

8 Loadability NULEODUR HTec highest loadability under acidic and basic conditions acidic conditions ph mg total load: mg. mg. mg. mg 6. mg. mg mg. mg. mg total load: mg HTec Gei Gei HTec 6 - total load: mg total load: mg 6. mg. mg. mg HTec HTec Gei Gei..... olumns: VP x mm NULEODUR - HTec, μm XI TM X, mm Gei μm Å Eluent: acetonitrile / formic acid in water, ph., (: v/v) Flow rate: ml/ Temperature: Detection: UV, nm Pressure: bar. -acetamido phenol (paracetamol). -acetamido phenol. acetylsalicylic acid Sample dissolved in DMSO. In comparison NULEODUR HTec shows no overloading or peak overlapping even at high sample concentrations. MN ppl. No. 9 basic conditions ph. 9 6 total load: mg total load: mg total load: mg 6. mg. mg 6. mg. mg. mg. mg HTec HTec HTec - Gei Gei Gei olumns: VP x mm NULEODUR - HTec XI TM X. mm Gei μm Å Eluent: methanol / mm NH HO, ph. (6:, v/v) Flow rate: ml/ Temperature: Detection: UV, nm Pressure: 6 bar hexobarbital. thiamylal XI TM and Gei are trademarks of Phenomenex no overloading effects even at maximum sample concentration no overlapping effects or intermixture of compounds high yields and efficiency MN..... Sample dissolved in DMSO. ompetitor's column provides no baseline separation even at medium load. MN ppl. No. 9

9 Loadability NULEODUR HTec optimal loadability by variation of ph acidic conditions ph. 6 mg 6 mg 9 mg -,,,,,, - -,,,,,,,,,,,, olumn: VP x mm NULEODUR - HTec Eluent: acetonitrile / acetic acid (:, v/v) ph:. Flow rate: ml/ Temperature: Detection: UV, nm. lidocaine. amitriptyline limited loadability under acidic conditions basic conditions ph. mg 6 mg 9 mg -,,, 6,,,, -,,, 6,,,, -,,, 6,,,, olumn: VP x mm NULEODUR - HTec Eluent: acetonitrile / NH HO in water (:, v/v) ph:. Flow rate: ml/ Temperature: Detection: UV, nm limited loadability at acidic conditions (ph.) at ph even 9 mg sample don t result in overloading effects highest yields and efficiency MN. lidocaine. amitriptyline significantly higher loadability by optimisation of ph at at 9 mg limit of solubility is reached long term stability at ph guaranteed!* *(see page ) 9

10 The VarioPrep System VarioPrep columns column system for preparative HPL manufactured from stainless steel with adjustable end fittings (suitable for frequent use of backflushing techniques) allows compensation of a dead volume, which could result at the column inlet after some time of operation at demanding conditions, without need for opening the column supplied with all NULEODUR and NULEOSIL spherical silicas The VarioPrep principle D Readjustment of fitting ased on our special packing procedure VarioPrep columns are produced with highest packing quality and bed density (). Due to intensively chemical and/or mechanical exposure of the column adsorbent, shrinking of the column bed can occur (; orange gap). In this even unlikely case readjustment of the VarioPrep column fitting (; turning the nut at column inlet clockwise) will eliate the emerged dead volume (D). The performance of the VarioPrep column is completely reconstituted and column lifetime is significantly extended. olumn Reconstitution Reconstitution of VarioPrep column performance: sligth peak broadening and deformation after injections under strongly demanding conditions (ph ; ; sample in DMSO) th injection just after readjustment of fitting readjustment of the column fitting restores the column performance and prolongs column lifetime noticeably Injections Injections approx. Injections vailable standard dimensions of VarioPrep columns with axially adjustable end fittings ID [mm] Length [mm] * * * * x x x x x x x x x x x 6 x x x x x x x x x x x x x x x x x x x x x x x x x x x end fitting design * x, x 6, x and x mm ID columns are used as guard columns and require the adequate holders. Details see on page. MN

11 VarioPrep Guard olumns The new guard column system for (semi-) preparative HPL easy handling and cartridge exchange robust hardware VP / for and mm ID columns VP /6 for 6 and mm ID columns VP / for and mm ID columns VP / for mm ID columns cost-efficient cartridges imally invasive / no disturbance of the separation efficiency of main column low back pressure Performance of preparative column when used with VarioPrep guard column VP /6 NULEODUR - HTec h-value:. symmetry:. Pressure: 6 bar VP /6 NULEODUR - HTec VP /6 NULEODUR HTec (guard column) h-value:.9 symmetry:. Pressure: bar Eluent: acetonitrile / water (:, v/v) Flow rate: 6 ml/ Temperature:. phenol. naphthaline. anthracene Using VarioPrep guard columns provides ideal protection of your main column symmetry, pressure and retention stay almost constant. Technical data: /6" thread free rotary plunger fittings low O-ring abrasion stainless steel Guard cartridge Recommended for column ID Prefered capillary ID Typical flow rate VP / and mm ID. and mm ml/ VP /6 6 and mm ID.,. and. mm ml/ VP / and mm ID.,. and. mm ml/ VP / mm ID. and. mm ml/ The cartridge system is designed for pressures up to bar. MN

12 Further NULEODUR Phases MHEREY-NGEL offers all NULEODUR phases as VarioPrep-columns in preparative dimensions. Gravity: % USP L % USP L modification with polar endcapping % USP L bifunctional RP phase, propylpheny and ligands; endcapping % ; USP L and L octadecyl / octyl phase, medium density coating endcapping ec:. % USP L ec:. % USP L zwitterionic ammonium sulfonic acid modification % % USP L ao phase for NP and RP separations. % USP L unmodified high purity silica USP L *= ph stability -, suitable for L/MS ph stability -, suitable for L/MS stabel against % aqueous eluents ph stability - 9, suitable for L/MS ph stability -, suitable for L/MS ph stability 9 ph stability,, suitable for L/MS n.a. hydrophobic selectivity, = OH OH Si O Si(H ) Si O Si(H ) cyano (nitrile) phase for NP and RP separations NULEODUR (Si-O)n octadecyl phase with specially crosslinked surface modification endcapping ph stability -, suitable for L/MS octadecyl phase, high density coating multi-endcapping Gravity: % USP L Structure NULEODUR (Si-O)n % USP L Stability NULEODUR NULEODUR NULEODUR (Si-O)n (Si-O)n (Si-O)n octadecyl phase, with high capacity, high density coating multi-endcapping haracteristics* NULEODUR (Si-O)n Specification ph stability, stable towards highly aqueous mobile phases ph stability, stable towards highly aqueous mobile phases suitable for L/MS ph stability polar/ionic selectivity, = NULEODUR NULEODUR NULEODUR NULEODUR (Si-O)n (Si-O)n (Si-O)n (Si-O)n Phase + N + N H H H H SO SO N N Si O Si(H ) NH NH steric selectivity MN

13 NULEODUR HTec Ordering Informations Lenght mm mm mm mm mm mm guard columns # analytical columns ( μm) mm ID mm ID mm ID mm ID # requires E adaptor REF 9 Lenght mm mm mm mm mm guard columns* preparative columns ( μm) mm ID mm ID mm ID mm ID mm ID Lenght mm mm mm mm mm guard columns* preparative columns ( μm) mm ID mm ID mm ID mm ID mm ID Lenght mm mm mm mm mm guard columns* preparative columns ( μm) mm ID mm ID mm ID mm ID mm ID Lenght mm mm mm mm Pack of Holder *preparative guard columns for NULEODUR HTec and holders mm ID mm ID 69.6 mm ID 69. mm ID 69. NULEODUR HTec bulk packings Pack of g Pack of kg NULEODUR - HTec.. NULEODUR - HTec.. NULEODUR - HTec.. For all questions about hromatography: Tel.: tech-chroma@mn-net.com MN

14 Please ask for further literature: Local distributor: Your contact to MN for all requests: NucDurHTec en///. PD Printed in Germany KTEN MHEREY-NGEL EN ISO 9: ERTIFIED MHEREY-NGEL GmbH & o. KG Neumann-Neander-Str. 6- D- Düren Germany Germany and international: Tel.: +9 () 96 9 Fax: +9 () sales-de@mn-net.com Switzerland: MHEREY-NGEL G Tel.: + () 6 Fax: + () 6 sales-ch@mn-net.com France: MHEREY-NGEL EURL Tel.: + () 6 6 Fax: + () 6 sales-fr@mn-net.com US: MHEREY-NGEL Inc. Tel.: + 9 Fax: + sales-us@mn-net.com