Perfluoroalkyl Acids (PFAAs) in Plasma of the West Indian Manatee (Trichechus manatus)
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1 Perfluoroalkyl Acids (PFAAs) in Plasma of the West Indian Manatee (Trichechus manatus) 6 th Florida Marine Mammal Conference March 29, 2018 Orlando, Fl John A. Bowden National Institute of Standards and Technology Hollings Marine Laboratory, Charleston, SC Background PFAA Exposure Health Metric 1
2 Courtesy HDR Architecture, Inc./Steve Hall Hedrich Blessing National Metrology Institute Gaithersburg, Maryland Hollings Marine Laboratory Improve environmental measurements Marine animal & human health Pollution Hazardous algal blooms Aquaculture Exposure science Mission promote U.S. innovation and industrial competitiveness by advancing measurement science, standards, and technology Charleston, South Carolina 2
3 My Research Pursuant of innovative analytical strategies (mass spectrometry) for investigating the impact of the environment on wildlife and humans 70-90% of chronic disease mortality is from environmental influence Adapt exposure model (internal <-> external measures <-> health) Chemical Strategies Targeted Targeted Untargeted Species Matrices PFAAs Hormones Lipidomics Manatee Plasma PAHs Eicosanoids Metabolomics Dolphin Tissues OCs/BPA Bile Acids Proteomics Various Fish Skin Trace Metals Vitamins Exposomics Birds DBS Phthalates Fatty Acids Toxins Crocodilians Urine 3
4 Manatee Project Establish collaborations -> get access to samples -> pilot studies Wildlife sentinels can be excellent models for assessing exposure Health Baseline Photo from: Robert Bonde, USGS Sirenia Project 4
5 Perfluoroalkyl Acids in Brevard County PFAA Burden in American Alligator at MINWR PFAA Burden in Manatees?? FPL Cape Canaveral Power Plant ng/g Environ Toxicol Chem. 2017;36(4): PFOS 2140 ng/g!! Chemosphere Jan;166:
6 What are Perfluoroalkyl acids PFAAs? High chemical and thermal stability Various uses/mechanisms enter environment Bioaccumulate, ubiquitous despite stopped production High publicity Perfluorooctanesulfonic acid (PFOS) 6
7 PFAAs Measured 7
8 PFAA Burden Seven PFAAs detected in manatees PFOS and PFNA 100% * * 8
9 PFAA Burden (ex., PFOS Range) ng/g Green sea turtle ng/g West Indian manatee ng/g Leatherback sea turtle ng/g Kemps Ridley sea turtle ng/g American alligator ng/g Bottlenose dolphin ng/g Minke whales ng/g wet mass 9
10 Biomarkers of PFAA Exposure/Health Effects? Cholesterol levels in manatees 10
11 Lipids and PFAAs: Connection? Endocrine Disrupting Chemicals, MDCs, and obesogens Peroxisome proliferator-activated receptor (PPAR, α and γ) Several exposure health outcomes (lab-based models) 11
12 Utility of the lipidome Stearic acid (18:0) Arachidonic acid (20:4) Tristearin (18:0/18:0/18:0) 8 categories 60 classes 254 subclasses 37,000 lipids in Lipid Maps Phosphatidylcholine (16:0/18:1) Utility of lipidomics? 12
13 Human <-> Environment Detection of specific lipid alterations has become a vital strategy for assessing both normal and pathological conditions in humans Exposure HABs Development Health
14 Manatee Plasma Lipidome Establish baseline 2,136 features (25 µl) Plasmanyl TAG, OXPls, 4 Ceramide, 22 Ceramide, 4 Glucosylceramide, 43 4 OxTAG, 6 Plasmenyl TAG, 4 CoQ, 5 Acylcarnatine, Sphingomyelin, 12 Diacylglycerol, 13 2 OxPC, Sphingomyelin, 3 53 MGDG, Phosphatidic acid, Phosphatidylglycerol, confirmed lipids 704 in progress lipids What do with profile? OxTAG, 71 Triacylglycerol (TAG), 79 Triacylglycerol (TAG), 139 Plasmanyl PS, 8 Phosphatidylinositol, Lyso PE, 3 5 Sulfatide, 4 MMPE, DGDG, 1 1 Phosphatidylcholine OxPC, 94 (PC), 37 Phosphatidylcholine Plasmanyl PC, 20 (PC), 104 Plasmenyl PC, 4 Lyso PC, 7 Plasmenyl PC, 8 Lyso PC, 14 Phosphatidylethanolamine Plasmanyl PC, 20 DMPE, Plasmanyl 11 PE, 7 (PE), 13 DMPE, Plasmenyl 1 Plasmenyl PE, 1 PE, 5 Cholesteryl ester, 9 Plasmanyl PE, 1 Number of Lipid Species 14
15 Pilot Lipidomic Results *Brevard County Control (n=12) *Brevard County UME (n=22) *Brevard County CSS (n=21) UME CSS Crystal River Control (n=39) Everglades Control (n=8) Diet & PFAA *Margaret Hunter, USGS 15
16 Summary Determine lipid signatures/pathways expressed with cohorts Lipids are only one snapshot -> metabolomics and proteomics Translation to other wildlife species, humans 16
17 Acknowledgements Kady Palmer, REU, Eckerd College Jackie Bangma, MUSC USGS (Robert Bonde, Margaret Hunter) NIST Lipidomics/Metabolomics Team NASA Research Team 17
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