BactoReal Kits for detection of bacteria
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1 BactoReal Kits for detection of bacteria BactoReal Kit Bartonella spp. Detection of the glta gene of B. clarridgeiae, B. elizabethae, B. grahamii, B. henselae, B. koehlerae, B. quintana, B. volans and B. washoensis BactoReal Kit Bordetella bronchiseptica Detection of the bfra gene of B. bronchiseptica BactoReal Kit Bordetella parapertussis Detection of the insertion sequence IS1001 of B. parapertussis, and of some strains of B. bronchiseptica and B. holmesii BactoReal Kit Bordetella pertussis Detection of the insertion sequence IS481 of B. pertussis, B. holmesii and some strains of B. bronchiseptica BactoReal Kit Borrelia burgdorferi sensu lato (CE-IVD) Detection of the ospa gene of Borrelia species belonging to the Borrelia burgdorferi sensu lato complex BactoReal Kit Brucella spp. Detection of the IS711 region of B. abortus, B. ovis, B. melitensis, B. suis and B. canis DHUB03953 DHUB02453 DHUB00253 DHUB00353 DHUB05053 DHUB02153 BactoReal Kit Chlamydophila pneumoniae Detection of the ompa gene of C. pneumoniae DHUB00953 BactoReal Kit Chlamydophila psittaci/abortus Detection of the omp1 gene of C. abortus and C. psittaci BactoReal Coming Soon Kit Clostridium difficile Toxins Detection and differentiation of toxin A (tcda), toxin B (tcdb) and binary toxin gene of C. difficile BactoReal Kit E. coli Toxin Typing Coming soon Detection of the toxin genes stx1, stx2 (verotoxin), eae (intimin) and ehxa (enterohemolysin) of E. coli BactoReal Kit Haemophilus influenzae (CE-IVD) Detection of the ompp6 gene of H. influenzae & VIC & VIC DHUB03053 DHUB04253 DHUB02753 DHUB01053 BactoReal Kit Legionella pneumophila (CE-IVD) Detection of the 23S-5S rdna intergenic spacer region of L. pneumophila BactoReal Kit Leptospira spp. (16S rdna) Detection of the 16S rrna gene of pathogenic and intermediately pathogenic Leptospira species BactoReal Kit Leptospira spp. (LipL32) Detection of the LipL32 gene of pathogenic Leptospira species BactoReal Kit Leptospira spp. Multiplex (16S rdna + LipL32) Detection and differentiation of LipL32 and 16S rrna gene of pathogenic and intermediately pathogenic Leptospira species VIC & VIC DHUB01153 DHUB00553 DHUB00653 DHUB00753 Human Diagnostics real-time PCR Kits Catalogue ingenetix Page 1 of 6
2 BactoReal Kit Listeria monocytogenes (CE-IVD) Detection of the hlya gene of L. monocytogenes DHUB00853 BactoReal Kit Mycoplasma pneumoniae (CE-IVD) Detection of the 16S rrna gene of M. pneumoniae DHUB01253 BactoReal Kit Neisseria gonorrhoeae (CE-IVD) Detection of the 16S rrna gene of N. gonorrhoeae DHUB01353 BactoReal Kit Neisseria meningitidis (CE-IVD) Detection of the ctra gene of N. meningitidis DHUB00153 BactoReal Kit Pseudomonas aeruginosa Detection of the 16S rrna gene of P. aeruginosa DHUB00263 BactoReal Kit Salmonella enterica Detection of the inva gene of S. enterica DHUB02563 BactoReal Kit Streptococcus agalactiae Detection of the cfb gene of S. agalactiae DHUB01553 BactoReal Kit Streptococcus pneumoniae (CE-IVD) Detection of the lyta gene of S. pneumoniae DHUB01453 BactoReal Kit Tropheryma whipplei Detection of the groel gene of T. whipplei DHUB01653 ViroReal Kits for detection of viruses ViroReal Kit Adenovirus (CE-IVD) Detection of the hexon gene of human adenovirus species A-G DHUV00153 ViroReal Kit Enterovirus (CE-IVD) Detection of RNA of the 5 UTR of enterovirus ViroReal Kit HDV Detection of the 5 UTR of human hepatitis D virus ViroReal Kit HHV-6A, B Detection of the U65/U66 gene of human herpes virus type 6 subtypes A and B ViroReal Kit HHV-6A Detection of the U65/U66 gene of human herpes virus type 6 subtype A ViroReal Kit HHV-6B Detection of the U65/U66 gene of human herpes virus type 6 subtype B ViroReal Kit HHV-7 Detection of the glycoprotein H gene of human herpes virus type 7 ViroReal Kit HHV-8 Detection of the ORF26 of human herpes virus type 8 DHUV00353 DHUV00953 DHUV00653 DHUV00453 DHUV00553 DHUV00753 DHUV00853 Human Diagnostics real-time PCR Kits Catalogue ingenetix Page 2 of 6
3 ViroReal Kit Human Coronavirus alpha Detection of RNA of the N gene of human coronavirus NL63 and 229E ViroReal Kit Human Coronavirus beta Detection of RNA of the N gene of human coronavirus HKU1, OC43, MERS ViroReal Kit Influenza A (CE-IVD) Detection of RNA of the matrix protein gene of influenza A virus ViroReal Kit Influenza B (CE-IVD) Detection of RNA of the hemagglutinin gene of influenza B virus ViroReal Kit Metapneumovirus* (CE-IVD) Detection of RNA of the nucleocapsid protein gene of hmpv A and hmpv B ViroReal Kit Parainfluenzavirus (CE-IVD) Detection of RNA of the HN gene of HPIV-1, HPIV-2, HPIV-3, HPIV-4 ViroReal Kit Parechovirus Detection of RNA of the polyprotein gene of human parechovirus strains 1-6 ViroReal Kit Parvovirus B19 Detection of the VP1 gene of parvovirus B19 genotypes 1, 2 and 3 ViroReal Kit Rhinovirus (A, B, C) (CE-IVD) Detection of RNA of the 5 UTR of human rhinovirus A, B and C ViroReal Kit Rotavirus Detection of RNA of the NSP4 gene of rotavirus strains genotype A, most strains of genotype B, C and some strains of the other genotypes D-G ViroReal Kit RSV (A & B) (CE-IVD) Detection of RNA of the nucleocapsid protein gene of human respiratory syncytial virus subtypes A & B ViroReal Kit VZV Detection of the ORF29 of varicella zoster virus & VIC & VIC & VIC DHUV01153 DHUV01253 DHUV01353 DHUV01453 DHUV01553 DHUV01653 DHUV01753 DHUV01853 DHUV01953 DHUV02053 DHUV02153 DHUV02253 *not available in BE, DE, DK, ES, FR, GB, IT, LI, NL, AU, CN, JP, KR, TW, US, PL, NO, CH, HU, IE, LU, TR ParoReal Kits for detection of parasites ParoReal Kit Acanthamoeba Detection of the 18S rrna gene of Acanthamoeba DHUP00153 Human Diagnostics real-time PCR Kits Catalogue ingenetix Page 3 of 6
4 General information for ingenetix kits Kit contents (50 reactions): Assay (primer + probe) for detection of pathogen Assay (primer + probe) + IPC target for detection ( channel) of Internal Positive Control Amplification Mix Positive Control Water Validated PCR-platforms: Applied Biosystems 7500 instrument (Thermo Fisher Scientific) LightCycler 480 (Roche) Mx3005P QPCR System (Agilent) Kits are optimized to run under same thermal cycling conditions RNA and DNA material can be analysed in one run H. pylori ClariRes Assay Product Name Channel Pathogen & IPC Order Number H. pylori ClariRes Assay (CE-IVD) Detection of the 23S rrna gene of H. pylori and determination of its clarithromycin resistance. IvD certified. RTGM100 Contents (50 reactions): Assay (primer + probe) for detection of pathogen and of internal positive control Positive Controls Internal Positive Control Target Water PCR-platform: Only LightCycler 1.2/1.5/2.0 on basis of melting curve analysis Attention: Amplification mix is not included. Recommended amplification mix: LC TM -FastStart DNA Master SYBR Green I Human Diagnostics real-time PCR Kits Catalogue ingenetix Page 4 of 6
5 MycoReal assays for detection of fungi Product Name Channel Pathogen & IPC Order Number MycoReal Aspergillus Detection of the ITS2-region of A. fumigatus, A. flavus, A. nidulans, A. niger and A. terreus PCR-platform: Only LightCycler 2.0 Recommended amplification mix (not included): LC TM -FastStart DNA Master Kit Hybridisation Probes Aspergillus: 640 nm, 705 nm RTPM100 MycoReal Candida Detection of the ITS2-region of C. albicans, C. dubliniensis, C. glabrata, C. krusei, C. lusitaniae, C. parapsilosis, and C. tropicalis PCR-platform: Only LightCycler 2.0 Recommended amplification mix (not included): LC TM -FastStart DNA Master Kit Hybridisation Probes Candida: 640 nm, 670 nm, 705 nm RTPM200 MycoReal Fungi Detection of the ITS2-region of fungi (panfungal), species identification via sequencing and BLAST analysis PCR-platform: Only LightCycler 2.0 Recommended amplification mix (not included): LC TM -FastStart DNA Master Kit Hybridisation Probes MycoReal Pneumocystis Detection of the mt LSU gene of Pneumocystis jirovecii PCR-platform: LightCycler 1.2/1.5/2.0/480, ABI PRISM 7500, Mx3005P Recommended amplification mix (not included): For LightCycler 2.0: LC TM -FastStart DNA Master Kit Hybridisation Probes For Applied Biosystems 7500, Mx3005P, LightCycler 480: TaqMan Gene Expression Master Mix Fungi: 640 nm Pneumocystis: IPC: VIC / HEX IPC: RTPM300 RTPM400 or RTPM401 or RTPM403 General information for MycoReal assays Content: Assay (primer + probe) for detection of pathogen (50 reactions) Assay for detection of internal positive control Positive Control(s) Attention: Amplification mix is not included Recommended amplification mix, depending on PCR-platform LC TM -FastStart DNA Master Kit Hybridisation Probes for LightCycler 1.2/1.5/2.0 TaqMan Gene Expression Master Mix for Applied Biosystems 7500, Mx3005P, LightCycler 480 Positive control standards Product Name BactoReal Pseudomonas aeruginosa Standard Contains a fragment of P. aeruginosa DNA at a concentration of 10 7 copies/μl. Only for use with BactoReal Kit Pseudomonas aeruginosa BactoReal Salmonella enteria Standard Contains a fragment of S. enterica DNA at a concentration of 10 7 copies/μl. Only for use with BactoReal Kit Salmonella enterica Order Number PC900 PC600 Human Diagnostics real-time PCR Kits Catalogue ingenetix Page 5 of 6
6 Assays for detection of internal positive control For use with ingenetix assays or customer assays Product Name Channel Order Number ControlReal 1 Detection of internal control DNA target (in assay mix). Recommended for Applied Biosystems 7500, Mx3005P ControlReal 2 Detection of internal control DNA target (in assay mix). Recommended for LightCycler 1.2/1.5/2.0 ControlReal 3 Detection of internal control DNA target (in assay mix). Recommended for Applied Biosystems 7500, LightCycler 480, Mx3005P Internal Positive Control Assay 1 Detection of internal control DNA target (in extra tube). Recommended for Applied Biosystems 7500, Mx3005P Internal Positive Control Assay 2 Detection of internal control DNA target (in extra tube). Recommended for LightCycler 1.2/1.5/2.0 Internal Positive Control Assay 3 Detection of internal control DNA target (in extra tube). Recommended for Applied Biosystems 7500, LightCycler 480, Mx3005P Internal Positive RNA Control Assay 1 Detection of internal control RNA target (in extra tube). Recommended for Applied Biosystems 7500, Mx3005P Internal Positive RNA Control Assay 2 Detection of internal control RNA target (in extra tube). Recommended for LightCycler 1.2/1.5/2.0 Internal Positive RNA Control Assay 3 Detection of internal control RNA target (in extra tube). Recommended for Applied Biosystems 7500, LightCycler 480, Mx3005P VIC / HEX NED (F2, 610 nm) VIC / HEX NED (F2, 610 nm) VIC / HEX NED (F2, 610 nm) RTGMCR-1 RTGMCR-2 RTGMCR-3 RTGMIPC-1 RTGMIPC-2 RTGMIPC-3 RTGMIPCR1 RTGMIPCR2 RTGMIPCR3 Human Diagnostics real-time PCR Kits Catalogue ingenetix Page 6 of 6
BactoReal Kits for detection of bacteria
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