High End Innovation in Molecular Diagnostics PRODUCTS Product Catalogue 09/ /2016
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1 High End Innovation in Molecular Diagnostics PRODUCTS Product Catalogue 09/ /2016
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3 Features Single and multiplex real (RT-) PCR time kits Can be run on a wide range of real time PCR instruments Compatible with different extraction procedures (incl. automation) Internal extraction control Complete test kits Validated in commercially available QA programs Recommended Real time PCR instruments: Agilent Mx 3000P, Mx 3005P Applied Biosystems: ABI 7500 und 7900, Step One Cepheid: Smartcycler Qiagen: Rotor-Gene Q Roche: LC 480 Sacace: SaCycler- Bio Rad: CFX 1
4 TABLE OF CONTENT REAL TIME (RT-) PCR KITS HUMAN VIROLOGY Cytomegalovirus 5 Enterovirus 5 gastroplexvirus 5 Norovirus (Genogroup I and II) 5 Parainfluenza 5 Respiratory DNA Multiplex 6 Respiratory RNA Multiplex 6 Respiratory Syncytial Virus 6 TBE Virus (Tick Borne Encephalitis, German: FSME) 6 HUMAN BACTERIOLOGY AND PARASITOLOGY Anaplasma phagocytophilum 7 Babesia species 7 Borrelia burgdorferi (sensu lato) 7 Campylobacter 7 Chlamydophila pneumoniae 7 gastroplexbac 7 Group B Streptococcus 8 Helicobacter pylori 8 Ehrlichia species 8 Legionella species 8 Legionella pneumophila 8 Listeria monocytogenes 9 MRSA 9 Mycoplasma pneumoniae 9 Respiratory DNA Multiplex 9 Salmonella enterica 9 HUMAN GENETIC PREDISPOSITION Lactose Intolerance 10 VETERINARY VIROLOGY BVDV (Bovine Virus Diarrhoea) 11 PRRSV (Porcine Respiratory and Reproductive Syndrom Virus) 11 SBV (Schmallenberg Virus) 11 VETERINARY BACTERIOLOGY AND PARASITOLOGY Borrelia burgdorferi (sensu lato) 12 Ehrlichia species 12 Mycobacterium avium ssp. paratuberculosis (Johne s disease) 12 2
5 PATHOGEN DETECTION IN TICKS Borrelia burgdorferi (sensu lato) 13 Ehrlichia species 13 TBE Virus (Tick Borne Encephalitis Virus, German: FSME) 13 ENVIRONMENT AND FOOD VIROLOGY Enterovirus 14 Norovirus (Genogroup I and II) 14 ENVIRONMENT AND FOOD BACTERIOLOGY Legionella species 15 Legionella pneumophila 15 Listeria monocytogenes 15 Salmonella enterica 15 Sample PREPARATION diarellalifecheck 16 NukEx Nucleic Acid Release Reagent 16 NukEx Mag RNA/DNA 16 NukEx Pure RNA/DNA 16 NukEx Pure RNA/DNA 16 NukEx PLUS Nucleic Acid Release Reagent 16 NukEx Royal TS 17 NukEx Universal Dilution Buffer 17 NukEx Stop 17 REAGENTS AND ENZYMES Proteinase K 17 OTHERS SAMPLE PREPARATION NukEx Collection tube 18 NukEx Pestle 18 NukEx Bead 18 NukEx TS 18 OTHERS SAMPLING ixsave Tick Remover 19 3
6 REAL TIME (RT-) PCR KITS All our real time PCR and real time RT-PCR Kits are based on hybridization or hydrolysis probe technology and can be used on all common real time PCR cyclers. The kits do not contain ROX as a reference dye but they all include an Internal Control (IC) or an Extraction Control (EC). Kits for LightCycler are designated for LightCycler 1.5, 2.0 and 480II. Kits for open real time PCR Instruments are designated for Instruments such as ABI Prism 7500 SDS and 7500 Fast SDS, m2000rt, LightCycler 480 Instrument II, Rotor-Gene 3000/6000, Rotor-Gene Q5/6 plex Platform, Mx 3005P QPCR System, and equivalent instruments. 4
7 HUMAN VIROLOGY Product Name Rxn For LightCycler For open real time PCR Cytomegalovirus virellacmv real time PCR Kit LC / TM G G For the detection of Human Cytomegalovirus. For Research Use Only G G Enterovirus New! virellaentero 2.0 LC/ TM real time RT-PCR Kit G010- G For the detection of Enterovirus (Coxsackievirus A, Coxsackievirus B, Echovirus, numbered Enteroviruses, Polioviruses). G010- G gastroplexvirus gastroplexvirus real time RT-PCR Kit G For the detection and differentiation of Norovirus (Genogroup I & II), Adenovirus and Rotavirus. G gastroplexvirus PLUS real time RT-PCR Kit G For the detection and differentiation of Norovirus (Genogroup I & II), Adenovirus, Rotavirus, Astrovirus and Sapovirus. G available autumn 2015 Norovirus (Genogroup I and II)* virellanoro real time RT-PCR Kit LC EC / TM EC For the detection of Norovirus (Genogroup I and II). G01007EC- G01008EC- *For the detection of Norovirus also see gastroplexvirus Multiplex real time RT-PCR. Parainfluenza respiraparaflu real time RT-PCR Kit For the detection of Parainfluenza Viruses 1, 2, 3 & 4 coming soon G G
8 Product Name Rxn For LightCycler For open real time PCR Respiratory DNA Multiplex respiradna real time PCR Kit Screening assay for the detection of Adenovirus, Mycoplasma pneumoniae, and Chlamydophila pneumoniae by multiplex real time PCR. For Research Use Only G G Respiratory RNA Multiplex respirarna 2.0 real time RT-PCR Kit Screening assay for the detection of Influenzavirus A and B, and Respiratory Syncytial Virus by multiplex real time RT-PCR. G G Respiratory Syncytial Virus* virellarsv real time RT-PCR Kit For the detection of Respiratory Syncytial Virus. CE-IVD pending G G *For the detection of Respiratory Syncytial Virus also see respirarna 2.0 Multiplex real time RT-PCR. TBE Virus (Tick Borne Encephalitis, German: FSME)* virellatbe real time RT-PCR Kit LC / TM G G For the detection of Tick Borne Encephalitis Virus. G G *For the detection of TBE Virus also see ixsave TBE real time RT-PCR. 6
9 HUMAN BACTERIOLOGY AND PARASITOLOGY Product Name Rxn For LightCycler For open real time PCR Anaplasma phagocytophilum* *for the detection of A. phagocytophilum see Ehrlichia spec. real time PCR Kits Babesia species diarellababesia real time PCR Kit For the detection of Babesia divergens, Babesia microti, and Babesia sp. EU1. For Research Use Only G G Borrelia burgdorferi (sensu lato)* diarellaborrelia real time PCR Kit TM For the detection of Borrelia burgdorferi (sensu lato). G G *For the detection of Borrelia burgdorferi also see ixsave Borrelia real time PCR. Campylobacter* diarellacampylobacter real time PCR For the detection of Campylobacter jejuni. coming soon G G *For the detection of Campylobacter also see gastroplexbac Multiplex real time PCR. Chlamydophila pneumoniae* diarellachlamydophila real time PCR Kit For the detection of Chlamydophila pneumoniae coming soon G G *For the detection of Chlamydophila pneumoniae also see respiradna Multiplex real time PCR. gastroplexbac gastroplexbac real time PCR Kit Screening assay for the detection of Listeria monocytogenes, Campylobacter jejuni, Salmonella enterica G G
10 Product Name Rxn For LightCycler For open real time PCR Group B Streptococcus New! diarellagbs real time PCR Kit For the detection of Group B Streptococcus CE-IVD pending G G Helicobacter pylori diarellahelicobacter real time PCR Kit LC For the detection of Helicobacter pylori and the simultaneous determination of its resistance status to clarithromycin. G G Ehrlichia species diarellaehrlichia real time PCR Kit For the detection of Ehrlichia chaffeensis, Ehrlichia ewengii and Anaplasma phagocytophilum (formerly known as Ehrlichia phagocytophilum, Ehrlichia equi). For Research Use Only G G Legionella species diarellapanlegionella real time PCR Kit LC For the detection of Legionella spec. by real time PCR. Differentiation of L.pneumophila by melting curve analysis. G G diarellapanlegionella real time PCR Kit TM G For the detection of Legionella spec. G Legionella pneumophila diarellalegionella real time PCR Kit LC / TM G G For the detection of Legionella pneumophila. G G
11 Product Name Rxn For LightCycler For open real time PCR Listeria monocytogenes diarellalisteria real time PCR Kit For the detection of Listeria monocytogenes. G G MRSA diarellamrsa 2.0 TM real time PCR Kit For the detection of Methicillin-resistant Staphylococcus aureus (MRSA) CE-IVD pending G G Mycoplasma pneumoniae* diarellamycoplasma real time PCR Kit For the detection Mycoplasma pneumoniae. G G *For the detection of Mycoplasma pneumoniae also see respiradna Multiplex real time PCR. Respiratory DNA Multiplex respiradna real time PCR Kit TM Screening assay the detection of Adenovirus, Mycoplasma pneumoniae, and Chlamydophila pneumoniae by multiplex real time PCR. For Research Use Only G G Salmonella enterica* diarellasalmonella real time PCR Kit For the detection of Salmonella enterica. G G *For the detection of Salmonella enterica also see gastroplexbac Multiplex real time PCR. 9
12 HUMAN GENETIC PREDISPOSITION Product Name Rxn For LightCycler For open real time PCR Lactose Intolerance Dr. Schröders LaktaseCheck real time PCR Kit For the detection of the C/T (-13910) polymorphism within the regulatory region of the lactase gene by melting curve analysis. G
13 VETERINARY VIROLOGY Product Name Rxn For LightCycler For open real time PCR BVDV (Bovine Virus Diarrhoea) virellabvdv 2.0 real time RT-PCR Kit FLI-B 637 For the detection of Bovine Virus Diarrhea Virus by real time RT-PCR. Sample material: RNA isolated from single or pooled bovine blood, serum, plasma and ear notch samples. Approved by the Friedrich-Loeffler-Institute 100 G01079 PRRSV (Porcine Respiratory and Reproductive Syndrom Virus) virellaprrsv real time RT-PCR Kit For the detection of PRRS Virus by real time RT-PCR. Sample material: RNA isolated from blood, saliva and tissue samples of swine. available autumn 2015 G01102 SBV (Schmallenberg Virus) virellasbv real time RT-PCR Kit FLI-B 653 For the detection of Schmallenberg Virus by real time RT-PCR. Sample material: RNA isolated from blood and tissue samples of ruminants. Approved by the Friedrich-Loeffler-Institute G
14 VETERINARY BACTERIOLOGY AND PARASITOLOGY Product Name Rxn For LightCycler For open real time PCR Borrelia burgdorferi (sensu lato) diarellaborrelia vet real time PCR Kit For the detection of Borrelia burgdorferi (sensu lato). G G Ehrlichia species diarellaehrlichia PLUS canine real time PCR Kit For the detection of Ehrlichia spec. (E. canis, E. chaffeensis, E. ewingii) and Anaplasma spec. (A. platys, A. phagocytophilum). G G diarellaehrlichia canine real time PCR Kit For the differentiation of Ehrlichia spec. (E. canis, E. chaffeensis, E. ewingii) and Anaplasma spec. To be used in combination with diarellaehrlichia PLUS canine real time PCR Kit TM (G and G01047-) G Mycobacterium avium ssp. paratuberculosis (Johne s disease) New! diarellamap vet real time PCR Kit FLI-B 673 For the detection of Mycobacterium avium ssp. paratuberculosis in fecal samples from Cattle and small ruminants (sheep, goat) and in culture samples. Approved by the Friedrich-Loeffler-Institute G
15 PATHOGEN DETECTION IN TICKS All ixsave real time (RT) PCR Kits contain primers and probes for the amplification of the 16S rrna gene of ticks. This heterologous amplification system serves as a control of the DNA/RNA extraction as well as for the control of the real time (RT) PCR. Product Name Rxn For LightCycler For open real time PCR Borrelia burgdorferi (sensu lato) ixsave Borrelia real time PCR Kit TM For the detection of Borrelia burgdorferi (sensu lato). G G ixsave Borrelia vet real time PCR Kit For the detection of Borrelia burgdorferi (sensu lato). G G Ehrlichia species* ixsave Ehrlichia real time PCR Kit For the detection of Ehrlichia chaffeensis, Ehrlichia ewengii and Anaplasma phagocytophilum (formerly known as Ehrlichia phagocytophilum, E. equi). G G ixsave Ehrlichia PLUS canine real time PCR Kit For the detection of Ehrlichia spec. (E. canis, E. chaffeensis, E. ewingii) and Anaplasma spec (A. platys, A. phagocytophilum) G G ixsave Ehrlichia canine real time PCR Kit For the detection of Ehrlichia spec. (E. canis, E. chaffeensis, E. ewingii). G G *For the detection of Ehrlichia phagocytophilum and E. equi see Anaplasma real time PCR Kits TBE Virus (Tick Borne Encephalitis Virus, German: FSME) ixsave TBE real time RT-PCR Kit LC / TM G G For the detection of Tick Borne Encephalitis Virus. G G
16 ENVIRONMENT AND FOOD VIROLOGY Product Name Rxn For LightCycler For open real time PCR Enterovirus New! virellaentero 2.0 LC/ TM real time RT-PCR Kit G010- G For the detection of Enterovirus (Coxsackievirus A, Coxsackievirus B, Echovirus, numbered Enteroviruses, Polioviruses). G010- G Norovirus (Genogroup I and II) virellanoro real time RT-PCR Kit LC EC / TM EC For the detection of Norovirus (Genogroup I and II). G01007EC- G01008EC- 14
17 ENVIRONMENT AND FOOD BACTERIOLOGY Product Name Rxn For LightCycler For open real time PCR Legionella species diarellapanlegionella real time PCR Kit LC For the detection of Legionella spec. by real time PCR. Differentiation of L.pneumophila by melting curve analysis. G G diarellapanlegionella real time PCR Kit TM G For the detection of Legionella species. G Legionella pneumophila diarellalegionella real time PCR Kit LC / TM G G For the detection of Legionella pneumophila G G Listeria monocytogenes diarellalisteria real time PCR Kit G For the detection of Listeria monocytogenes. G Salmonella enterica diarellasalmonella real time PCR Kit G For the detection of Salmonella enterica. G
18 SAMPLE PREPARATION Product Name Package Soze Cat. No. diarellalifecheck diarellalifecheck Reagent for the selective differentiation of living and dead bacteria in drinking water and other watery sample material. 100 G01023 NukEx Nucleic Acid Release Reagent NukEx Nucleic Acid Release Reagent Reagent for the enzymatic release of nucleic acid from tissue samples, ticks and insects. 100 G01013 NukEx Mag RNA/DNA NEW! NukEx Mag RNA/DNA Magnetic bead-based system for the isolation of nucleic acid (RNA and DNA) from clinical specimens (e.g. blood, urine, tissue, stool, etc.), food and environmental samples G G NukEx Pure RNA/DNA NukEx Pure RNA/DNA Spin-column-based system for the isolation of nucleic acid (RNA and DNA) from clinical specimens (e.g. blood, urine, tissue, stool, etc.), food and environmental samples G G NukEx Pure RNA/DNA NEW! NukEx Pure RNA/DNA Microtiter plate spin-column-based system for the isolation of nucleic acid (RNA and DNA) from clinical specimens (e.g. blood, urine, tissue, stool, etc.), food- and environmental samples. 0 G05017 NukEx PLUS Nucleic Acid Release Reagent NukEx PLUS Nucleic Acid Release Reagent Reagent for the enzymatic release of nucleic acid from Gram-positive bacteria. Very simple and fast protocol! 100 G
19 Product Name Package Soze Cat. No. NukEx Royal TS NukEx royal TS (tissue shred) Ready-to-use 1.5 ml safe lock reaction tubes containing NukSand and NukEx Nucleic Acid Release Reagent for manual preparation of ticks and insects with a pestle (e.g. gerbion NukEx Pestle 1.5 ml Cat. No. G06006). NukEx royal TS (tissue shred) Ready-to-use 2.0 ml screw cap tubes containing NukSand and NukEx Nucleic Acid Release Reagent for the automated preparation of ticks and insects using e.g. FastPrep Cell Disrupter (qbiogene). NukEx royal TS (tissue shred) Ready-to-use 2.0 ml safe lock reaction tubes containing Nuk sand and NukEx Nucleic Acid Release Reagent for the automated preparation of ticks and insects using e.g. TissueLyser (Qiagen). 100 G G G05003 NukEx Universal Dilution Buffer NukEx Universal Dilution Buffer Universal buffer for the dilution of samples prone to PCR inhibition. 100 G01014 NukEx Stop NukEx Stop Stop reagent for use with NukEx and NukEx PLUS. 100 G05019 REAGENTS AND ENZYMES Product Name Package Soze Cat. No. Proteinase K Proteinase K from Tritirachium album (Molecular Biology Grade). 50 mg G
20 OTHERS SAMPLE PREPARATION Product Name Package Soze Cat. No. NukEx Collection tube Collection tubes suitable for use with NukEx Spin Columns. DNase-, RNase-, pyrogen-free. 500 G06008 NukEx Pestle 100 disposable PBTP pestles for use in 1.5 ml reaction tubes. DNase-, RNase-, pyrogen-free. 100 G06006 NukEx Bead New! NukEx Bead 2.0 ml screw cap tubes containing NukEx Bead for the homogenization of tissue samples and sample preparation for the detection of Mycobacteria using e.g. FastPrep Cell Disruptor. New! NukEx Bead 2.0 ml safe lock reaction tubes containing NukEx Bead for the homogenization of tissue samples and sample preparation for the detection of Mycobacteria using e.g. TissueLyser Instrument. 100 G06009-sc 100 G06009-sl NukEx TS NukEx TS 1.5 ml 1.5 ml safe lock reaction tubes containing NukSand for the homogenization of tissue samples. NukEx TS 2.0 ml 2.0 ml screw cap tubes containing NukSand for the homogenization of tissue samples using e.g. FastPrep Cell Disruptor. 100 G G
21 OTHERS SAMPLING Product Name Cat. No. ixsave Tick Remover ixsave tick test human Tick remover set. For further information please contact ixsave tick test dog Tick remover set. For further information please contact erbion.com. ixsave tick test horse Tick remover set. For further information please contact G06001 G06004 G
22 Notes: 20
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24 gerbion GmbH & Co. KG Remsstr Kornwestheim Germany fon: fax: info@gerbion.com Distribution in Germany AlphaScience GmbH Hauptstrasse Riedstadt Germany fon: fax: info@alphascience.de
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