Safe Moving Vaccine Factory. Huazhong Agricultural University(HZAU) 2013 igem Team

Transcription

1 Safe Moving Vaccine Factory Huazhong Agricultural University(HZAU) 2013 igem Team

2 Motivation Estimated numbers of deaths from rabies in deaths Asia Africa Others WHO Expert Consultation On Rabies

3 Possible solutions Massive vaccination of dogs Post-exposure prophylaxis High risk

4 Parasitic vaccine First idea

5 Final idea Insect injector Moving vaccine

6 Safe moving vaccine factory Flea Bacillus subtilis

7 Why we chose flea? Midgut Foregut Esophagus Foregut Yersinia pestis biofilms Blocked flea B.J. Hinnebusch et al 2008

8 Why we chose flea? Blocked flea Injected Bacteria B.J. Hinnebusch et al 2008

9 Why we chose B.subtilis? Advantages of spore vaccines For our project Characters Details Reasons Bacillus sp Delivery antigens Genetic manipulation Bacillus sp are found in flea Bacillus subtilis can form biofilms Spores is effective for oral delivery and can stimulate immune response It is a ideal chassis for secretion of protein. Yes Yes Yes Probiotics It is gut commensal and can suppress enteric pathogens History PA P1 TT-C LTB antigens Yes Yes M.Cutting S et al 2011 FERREIRA et al 2005

10 Rabies vaccine Rabies virus Morimoto K et al 1999

11 Antimicrobial peptides 38AA Microbicidal activity

12 Antimicrobial peptides 25AA

13 Can eat and vomit B.subtilis? GFP-tagged B.subtilis

14 Conclusion of genetic circuit Anti-rabies vaccine Safety issue Safe moving vaccine factory Trails and errors... Moving issue

15 Lactoferrin-25AA PHT304 shuttle vector B.subtilis 168WB800 Inhibition zone

16 Human lysozyme-38aa PHT304 shuttle vector B.subtilis 168WB800 I can do it. Inhibition zone

17 Fusion protein Fusion of human lysozyme and lactoferrin PHT304 shuttle vector B.subtilis 168WB800 Inhibition zone

18 For negative bacteria E.coli

19 For negative bacteria Salmonella

20 For positive bacteria Micrococcus lysodeikticus

21 For positive bacteria S.aureus

22 For all antimicrobial peptides I am free of pathogenic bacteria now! Gram-positive bacteria Gram-negative bacteria

23 Glycoprotein PHT304 shuttle vector B.subtilis 168WB800 Western blot

24 Conclusion 1.We submitted 9 parts K541501K K K K K Well-documented parts K Fusion K K K

25 Discussion What we have done for controlling rabies? Glycoprotein If Yes New rabies vaccine AMP Clean fleas Blocked fleas Health and medicine Modeling Safe moving vaccine factory

26 Modeling 1 Gray logistic 2 Immune response 3 Cellular automaton

27 Model 1 Gray logistic K is the largest population N t is the population r is the intrinsic rate of population growth Different bacilli dilution: Coat petri dishes: 10-5 : 3 petri dishes/30min 10-6 : 3 petri dishes/30min 10-7 : 3 petri dishes/30min Total: 8 hours

28 Wang.XY. et al Model 1 Gray logistic The posterior error reification is 0.19 lower than 0.35 (The posterior error is criminant parameter ), so the prediction accuracy is high. Fig 1. The predicted value ( measured value ( ) ) and

29 Model 2 Immune response The antibody and antigen s rate of change Fig 2A. The amount of antigen and antibody changes over time Fig 2B. The relations between antigen and antibody Y.Xia et al. 2013

30 Model 3 Cellular automaton Fig 3. The relations between the days to reach safe immunity level (70%)and the initial percentage of immune dogs

31 Model 3 Cellular automaton Fig 4C. The immunity distribution after 47 days. Fig 4B. The immunity distribution after 22 days. Fig 4A. The initial distribution (1.2%) of immunity.

32 Model 3 Cellular automaton Fig 5. The percentage of immune dogs varies over time 32

33 Safety Level Of Different Parts Evaluation item Laboratory Vector Receptor microorganisms Rabies glycoprotein Recombinant organism Ecological security Safety level I I I II II II I-- No danger III- Moderate risk II--low danger IV-- High Risk

34 Questionnaire Survey Q1: Do you accept the fleas carrying the bacteria expressing rabies glycoprotein? 48% Q2: If the fleas 1 only bite animals; 2 carry the modified bacteria which can kill the pathogens in the fleas' forestomach; 3 immune several kinds of diseases; How do you like them? 22% 52% 78%

35 Human practice

36

37 Publication

38 Twitter twitter twitter~ WHU-China HUST-China HZAU-China

39 Collaboration We have a meeting with HUST igem team And then.. 1. We shared our idea with them. 2. We help them to characterize their part BBa_K They help us characterize BBa_K

40 Other works Exhibition and explanation. Safety assessment of GM rice lecture. Visit Wuhankeqian company and learn how to produce vaccines.

41 Grand challenges Explorations(GCE) 1.GCE belongs to Bill and Melinda Gates Foundation s commitment. 2. It just funds innovative ideas! If success, we will have $100,000USD. Ambition: To make Asia and Africa free of rabies!

42 Acknowledgment

43 Sponsor Thanks to HZAU: Library College of life science and technology College of animal science and technology College of veterinary medicine Center for Bionfiformatics Center for Biology experiment teaching Thanks to Wuhankeqian Animal Biological Products Co.,Ltd Association of Sci-technology for College students State key laboratory of agricultural microbiology National Natural Science Foundation of China

44 Q & A