Lab Guide Chemistry Section Lab Guide

Transcription

1 Lab Guide Chemistry Section Lab Guide

2 Acetaminophen Full : Acetaminophen, Anacin-3, Datril, Liquiprin, Paracetamol, Panadol, Panex, Phenaphen, Tylanol, Tampra Short name: ACTM Blood Specimen Serum - Plain tube (Red or yellow). Plasma-Lithium heparin Tube ("To be drawn 4 hours after the ingestion ) 2-30 o C Routine: 8 Hours STAT: 1 Hour Immunoturbidometry Unit : μmol/l Therapeutic range Range : μmol/l Rejection criteria See Management of Laboratory Specimen (CL 7067)

3 ALBUMIN Full : ALBUMIN Short name: ALB Blood Specimen - Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube STAT :1 Hour Routine :8 Hours Endpoint Unit: g/l Male/ Female Yrs Yrs Yrs Yrs Yrs Yrs Rejection criteria See Management of Laboratory Specimen (CL 7067);

4 ALCOHOL Full : Ethanol, Ethyl Alcohol, Blood Alcohol Short name: ETOH (ALC) Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube 24/7 STAT STAT : 1 hour Enzymatic rate method Unit: mmol/l Age Female & Male Y < 0.87 Rejection criteria See Management of Laboratory Specimen (CL 7067);

5 ALKALINE PHOSPHATASE Full : ALKALINE PHOSPHATASE Short name: ALK PHOS Cerner name: ALP Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube STAT :1 Hour Routine :8 Hours Kinetic Rate. Unit: U/L Age : Female: Male: < 14D D 1Y Y 10Y Y 13Y Rejection criteria 13Y 15Y Y 17Y Y 19Y >19Y See Management of Laboratory Specimen (CL 7067);

6 ALT Full : Alanine aminotransferase Short name: ALT, sgpt Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube 2-30 ºC Routine: 8 Hrs STAT : 1 Hr Kinetic Rate Unit: U/L Female : 0-1Yrs Yrs Yrs 8-22 >18Yrs 0-55 Male: 0-1Yrs Yrs Yrs 8-22 >18Yrs 0-55 Rejection criteria See Management of Laboratory Specimen (CL 7067);

7 Ammonia Full : Ammonia Short name: NH3 Cerner name: AMM Full tube of blood in ice in lavender top tube with EDTA -Immediately place in ice In ice STAT/Routine :Sunday - Saturday (24/7 Days) STAT : 1 hr Endpoint Unit: umol/l Female: 1D-1M M-1Y Rejection criteria 1-14Y Y >18 Y Male : 1D-1M M-1Y Y Y >18Y See Management of Laboratory Specimen (CL 7067); - Samples received without ice

8 AMYLASE Full : AMYLASE (Pancreatic) Short name: P-AMY Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube Routine: 8 hours STAT : 1 hour Immuno inhibition method Unit: U/L Age Female : Age Male: 0Min-6M 0Min-6M 6-2Y Y Y Y Y Y Y Y Y Y Y Y Y 8-51 Rejection criteria See Management of Laboratory Specimen (CL 7067);

9 AST Full : Aspartate aminotransferase Short name: AST, sgot Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube Routine: 8 hours STAT: 1 hour Kinetic rate method Unit: U/L Age: Female & Male: 0-14 D D -1Y Y Y Y Y 5-34 Rejection criteria See Management of Laboratory Specimen (CL 7067);

10 B-HCG Full : Human chorionic gonadotropin (Beta subunit) Short name: B-HCG Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube 4 ml tube (Fill until the required mark) 2-25 o C STAT / Routine: Sunday-Saturday (24/7 Days) STAT : 2 hours Routine: 8 hours Chemiluminiscent Immunoassay Unit: IU/L Female/male: < 5 Rejection criteria See Management of Laboratory Specimen (CL 7067);

11 Beta-Hydroxybutyrate (Beta-Ketone) Full : Beta-Hydroxybutyrate (Beta-Ketone) Short name: B-OHB Blood specimen- Lithium heparin tube delivered to the lab within 60 minutes 2-30ºC STAT:30 minutes Electro-chemical (Optium sensor from Abbott) Unit: mmol/l Rejection criteria - Wrong container - Frozen blood - Transportation time exceeds 60 min from collection.

12 BICARBONATE Full : BICARBONATE Short name: CO2, TCO2 Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube Routine: 8 Hours STAT : 1 hour Diff. Rate PH Unit: mmol/l Age: Female Male: 0-1 Y Y Y Y Y Y Rejection criteria See Management of Laboratory Specimen (CL 7067);

13 Bilirubin (Direct) Full : Bilirubin (Direct) Short name: DBIL Blood Specimen Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube Routine: 8 hours STAT: 1 hour DIAZO Unit: mmol/l Age: Female & Male: 0-1 Y Y Y Y Y Y Rejection criteria See Management of Laboratory Specimen (CL 7067);

14 Bilirubin (Total) Full : Bilirubin (Total) Short name: TBIL Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube STAT :1 Hour Routine : 8 hours DIAZO-method Unit: μmol/l Age: Female & Male: 0 mts - 2D D D D - 150Y Rejection criteria See Management of Laboratory Specimen (CL 7067);

15 CALCIUM Full : CALCIUM, CALCIUM (Corrected) Short name: CA, CA-C Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube STAT: 1 Hour Routine : 8 Hours ISE:INDIRECT Unit: mmol/l Age: Female Male: 0-1 Y Y - 5Y Y - 10Y Y - 14Y Y 20Y >20 Y This is an estimate of calcium concentration, assuming a normal albumin concentration which is 40 g/l Rejection criteria See Management of Laboratory Specimen (CL 7067);

16 CARBAMAZEPINE Full : CARBAMAZEPINE, TEGRETOL Short name: CARB 2 ml blood in: - Plain tube without gel or Lithium heparin Tube *Recommended trough measurement, blood should be withdrawn just before next dosage 2-30 o C Routine: 8 hour STAT : 1 hour Immunoturbidimetry Therapeutic range Unit: μmol/l Therapeutic Range : Toxic Levels: μmol/l Toxic multiple drug Regimen > 34 μmol/l Toxic single drug Regimen >51 μmol/l Rejection criteria See Management of Laboratory Specimen (CL 7067);

17 CARBOXYHEMOGLOBIN Full : Carboxyhemoglobin Rejection criteria Short name: COHb Whole blood collected in - Lithium heparin tube or - Blood gas capillary kit Should be in ice STAT / Routine: Sunday Sunday ( 24/7 Days ) STAT: 30 minutes Potentiometry, amperometry, and conductance Unit: % Non Smoker : < 1.5 % of total Hb Smoker : % of total Hb Wrong container Samples not in Ice Sample received after 60 minutes of collection time

18 Chloride Full : Chloride Short name: CL Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube Routine: 8 Hours STAT: 1 hour Therapeutic range ISE:INDIRECT Unit: mmol/l Female& Male : >18Y Rejection criteria See Management of Laboratory Specimen (CL 7067);

19 Cholesterol Full : Cholesterol Short name: CHOL Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube Routine: 8 hours STAT : 1 hour Therapeutic range Timed Endpoint method Unit: mmol/l Female & Male : <5.17mmol/L Desirable mmol/l Border line-high > 6.18 mmol/l High Rejection criteria See Management of Laboratory Specimen (CL 7067);

20 CORTISOL Full : CORTISOL Short name: CORT Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube Rejection criteria 4 ml tube (Fill until the required mark) 2-25 o C STAT / Routine: Sunday - Thursday Routine : 2 days CHEMILUMINISCENT IMMUNOASSAY Unit: umol/l Age Female: Male: 0-5 D D-1 Y Y Y Y Y AM : PM : <276 See Management of Laboratory Specimen (CL 7067);

21 C-Reactive Protein Full : C-Reactive Protein Short name: CRP Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube 4 ml tube (Fill until the required mark) Routine: 8 Hours STAT: 1 hour Interpretation Immunoturbidometry This is not the high-sensitive CRP, it s not intended to be used for assessment of risk of future coronary heart diseases Unit :mg/l Female & Male: Rejection criteria < 5 See Management of Laboratory Specimen (CL 7067);

22 CK (Creatine Kinase) Full : Creatine Kinase Short name: CK; CPK Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube 4 ml tube (Fill until the required mark) STAT: 1 hour Routine :8 hours Enzymatic rate method Unit: U/L Age Female: Male: 0-3M M - 1Y Y - 3Y Rejection criteria 3Y - 6Y Y - 9Y Y - 12Y Y - 15Y Y - 18Y >18 Y See Management of Laboratory Specimen (CL 7067);

23 CREATININE Full : CREATININE Short name: CREA Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube 4 ml tube (Fill until the required mark) STAT: 1 hour Routine : 8 hours Rate Jaffe method Unit: μmol/l Age Female: Male: 0-1Y Y - 5Y Y - 10Y Y - 14Y Y - 20Y >20Y Rejection criteria See Management of Laboratory Specimen (CL 7067);

24 CSF GLUCOSE Full : CSF GLUCOSE Short name: CSF-G spinal fluid in clean sterile tube, if there is contamination with RBCs then preferred in fluoride tube. *It is recommended to collect plasma glucose at the same time o C Routine: 8 Hrs STAT: 1 Hr Therapeutic Range Rejection criteria Enzymatic UV (Hexokinase) Unit: mmol/l Normal range for CSF glucose is 60-80% of plasma glucose. See Management of Laboratory Specimen (CL 7067); -Gross presence of blood

25 CSF Protein Full : CSF Protein Therapeutic Range Short name: CSF-P 1 ml spinal fluid in clean sterile tube o C Routine: 8 Hrs STAT: 1 Hr Timed endpoint method Unit: g/l Male / Female : 0-7 Days Days D-150 Y Rejection criteria See Management of Laboratory Specimen (CL 7067); Gross presence of blood in CSF specimen invalidates the protein value.

26 CSF-LDH Full : CSF-LDH Short name: CSF-LDH 1 ml Spinal fluid in a sterile container 2-30 o C Routine: 8 Hrs STAT: 1 Hr Therapeutic Range Rejection criteria -Enzymatic Rate method (Lactate to Pyruvate.) Unit: IU/L About 10% of serum levels. See Management of Laboratory Specimen (CL 7067);

27 DIGOXIN Full : DIGOXIN,, Lanoxicaps, Lanoxin Short name: DGN 4 ml blood in: Lithium heparin tube *blood should be drawn pre-dose or at least 6 hrs post-dose 2-30 o C Routine: 8 Hrs STAT: 1 Hr Therapeutic Range Immunoturbidimetry Unit: nmol/ L

28 Therapeutic Range : nmol/ L Toxic Levels : > 2.6 nmol/l Panic Levels: > 3.8 nmol/l *Result should be considered in the clinical context, ex: renal function. *This test measures total digoxin and in case of treatment with digi band very false high value may occur. *In neonate and old people interefere form digoxin like substance may happen. Rejection criteria See Management of Laboratory Specimen (CL 7067); ESTRADIOL Full : ESTRADIOL Short name: ESTRADIOL Blood Specimen- Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube Therapeutic Range 4 ml tube (Fill until the required mark) STAT / Routine: Sunday - Thursday Routine: 2 Days Chemiluminiscent Immunoassay Unit: pmol/l Male: Female :

29 Follicular Midcycle Luteal Post Menstrual Rejection criteria See Management of Laboratory Specimen (CL 7067); Ferritin Full : Ferritin Short name: FRE Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube 4 ml tube (Fill until the required mark) STAT / Routine: Sunday - Thursday Routine: 2 Days Chemiluminiscent Immunoassay Age : Female: male: <5 Y

30 5 9 Y Y Y Y Rejection criteria See Management of Laboratory Specimen (CL 7067); Folate Full : Folate, Serum Short name: FOLS/Folic Acid Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube 4 ml tube (Fill until the required mark) STAT / Routine: Sunday - Thursday Routine: 2 Days Chemiluminiscent Immunoassay Unit: nmol/l Age Female Male

31 0 1 yr yrs yrs yrs yrs yrs >18 yrs Rejection criteria See Management of Laboratory Specimen (CL 7067); Follicle Stimulating Hormone Full : Follicle Stimulating Hormone HORMONE Short name: FSH Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube 4 ml tube (Fill until the required mark) STAT / Routine: Sunday - Thursday Routine: 2 Days Chemiluminiscent Immunoassay Unit: IU/L Age : Female: Male :

32 0-2 Y Y Y Y 1-19 Follicular :4-9 Midcycle : 5-23 Luteal : 2-5 Post menopausal : Rejection criteria See Management of Laboratory Specimen (CL 7067); Free PSA Full : Free PSA Short name: FPSA Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube 4 ml tube (Fill until the required mark) STAT / Routine: Sunday - Wednesday Routine: 2 Days Chemiluminiscent Immunoassay

33 Unit: ng/ml Rejection criteria PSA > 4ng/ml & FPSA/Total PSA ratio < 25% indicates increased risk of prostatic cancer. Free PSA is reflex testing if TPSA is between > < 10.0 ng/ ml. See Management of Laboratory Specimen (CL 7067); FREE THYROXINE Full : FREE THYROXINE Short name: FT4 Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube 4 ml tube (Fill until the required mark /Analyzer STAT / Routine: Sunday -Thursday Routine: 2 days Chemiluminiscent Immunoassay Unit: pmol/l Male & Female:

34 Age From Age To Sex Reference Range 0 Day <20 Days Both Days < 3 years Both years 19 years <19 years 150 years Both Both For Pregnant : 1 st Trimester 2 nd Trimester 3 rd Trimester Female Female Female Rejection criteria See Management of Laboratory Specimen (CL 7067); Performing Lab Location GGT Full : Gamma-glutamyltransferase Short name: GGT, g-gt Blood Specimen- Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube 4 ml tube (Fill until the required mark Routine: 8 Hrs STAT : 1 Hr Enzymatic rate method Unit: U/L

35 Age Female Male 0 7D D 1M M 4M M -7M M-1Y Y 5Y Y 10Y Y 18Y >18Y Rejection criteria See Management of Laboratory Specimen (CL 7067); Gentamycin Collection time Therapeutic Range Full : Gentamycin Short name: Genta Trough: Just prior to next dose Peak: 1 hour after the end of infusion 2 ml blood in: - Plain red tube or yellow tube 2-30 o C - Lithium heparin tube Routine: 8 Hrs STAT: 1 Hr Immunoturbidimetry Unit :μg/ml

36 Trough: < 2 μg/ml Peak: 5 10 μg/ml Rejection criteria See Management of Laboratory Specimen (CL 7067); Glucose Full : Glucose, Fasting Blood Glucose, Random Blood Glucose Short name: GLU, FBS, RBS Blood specimen Fasting blood or random blood glucose - Grey top tube or - Plain tubes (red or yellow top)

37 2 30 o C Routine: 8 Hrs STAT: 1 Hr Therapeutic Range Timed end point (Hexokinase) Unit: mmol/l Serum/Plasma : mmol / L Random Blood Glucose : mmol/l Categories of increased risk for diabetes: Fasting mmol/l 2hrs PG (75g) mmol/l Hb A1c % Criteria for the diagnosis of diabetes Hb A1c Fasting glu OR 2hrs PG (75g) OR Random PG OR (in a patient with classic symptoms of hyperglycemia) Rejection criteria See Management of Laboratory Specimen (CL 7067) Glucose Tolerance Test (75 gms) Full : Glucose Tolerance Test (75 gms) Short name: GTT-75 gms 2 ml blood in grey top- flouride tube: GTT1 (for pregnant) - Fasting blood is extracted - Ingestion of 75 gm glucose solution - Two samples are collected after glucose solution administration at the following timings: 1hr, 2hrs 2 ml blood in grey top-flouride tube: GTT2 (for adult non-pregnant/male)

38 - Fasting blood is extracted - Ingestion of 75 gm glucose solution - Sample is collected after 2 hours of oral glucose intake 2 30 o C Routine: 8 Hrs STAT: 1 Hr Timed end point (Hexokinase) 75 gm for pregnant: The diagnosis of gestational diabetes mellitus is made when any of the following plasma glucose values are exceeded: o Fasting 5.1 mmol/l o 1 hr 10.0 mmol/l o 2 hr 8.5 mmol/l 75 gm for adult non-pregnant/male o Fasting between mmol/l is impaired fasting. o Two hours between is impaired glucose tolerance. o If fasting is 7.0mmol/l or two hours is 11.1mmol/l, diagnosis of diabetes. Rejection criteria See Management of Laboratory Specimen (CL 7067); Hemoglobin A1c Full : Hemoglobin A1c, Glycated Hb, Glycated Hemoglobin Short name: HbA1c EDTA whole blood. SUN-THU (08:00 14:00)

39 Routine : 3 days Rejection criteria Colorimetric / Turbidimetric Unit :% Female / MALE Normal range: ( ) % HbA1C See Management of Laboratory Specimen (CL 7067); -Clotted blood sample. HDL- Cholesterol Full : High Density Lipoprotein Cholesterol Short name: HDL, HDL-C Blood Specimen - Serum - Plain tube (Red or yellow). - Plasma - Lithium heparin tube

40 Routine: 8 Hrs STAT : 1 Hr Hemogeneous,colorimetric Unit: mmol/l FEMALE / MALE > 1.00 mmol/l Rejection criteria See Management of Laboratory Specimen (CL 7067) IRON Full : IRON Short name: Fe Blood Specimen - Serum - Plain tube (Red or yellow).

41 - Plasma - Lithium heparin tube Routine: 8 Hrs STAT: 1 Hr Timed Endpoint Unit: μmol/l Age Female Male 0-14 Y Y-19 Y Y-150 Y Rejection criteria See Management of Laboratory Specimen (CL 7067); -Hemolysed samples Lactate ( Lactic Acid) Full : Lactate ( Lactic Acid) Short name: LA

42 Whole blood collected in syringes /tubes containing dry Lithium Heparin Minimum 200 µl in 1mL Syringe,Minimum 2ml in 4ml tube. STAT: 1 hr Potentiometry,Amperometry,Conductance Unit: mmol/l 0 3 M M 2Y Y 18Y >18Y Rejection criteria Clotted samples, Samples with unacceptable anticoagulant. LDH - Lactate Dehydrogenase

43 Full : Lactate Dehydrogenase Short name: LDH Blood Specimen - Serum - Plain tube (Red or yellow). - Plasma - Lithium heparin tube Routine: 8 hrs STAT : 1 Hr Enzymatic rate method (Lactate to Pyruvate) Unit: U/L Age Female: Male: 1-14 D D-1 Y Y Y Y Y Rejection criteria See Management of Laboratory Specimen (CL 7067). LIPASE

44 Full : LIPASE Short name: LIP Blood Specimen - Serum - Plain tube (Red or yellow). - Plasma - Lithium heparin tube Routine: 8 Hrs STAT: 1 Hr Enzymatic rate method Unit: U/L Age : Female: Male: 0-19 Y Y Rejection criteria See Management of Laboratory Specimen (CL 7067);

45 LDL - Cholesterol Full : Low Density Lipoprotein-Cholesterol (calculated) Short name: LDL-C - Recommended to order with cholesterol, triglyceride and HDL at the same time. Blood Specimen - Serum - Plain tube (Red or yellow). - Plasma - Lithium heparin tube Routine: 8 Hrs STAT : 1 Hr Calculated Unit: mmol/l Adults Desirable: < 3.36 Borderline: High Risk: > 4.12 Rejection criteria See Management of Laboratory Specimen (CL 7067);

46 LUTEINIZING HORMONELUTEINIZING Full : LUTEINIZING HORMONELUTEINIZING MONEUTEINIZING HORMONE Short name: LH Blood specimen: Serum - Plain red tube or yellow SST (2 ml - 5 ml) Plasma - Lithium heparin tube 4 ml tube (Fill until the required mark) STAT / Routine: Sunday - Thursday Routine: 2 Days Chemiluminiscent Immunoassay Unit: IU/L Age : Female: Male: 0-1 Y Y Y Y >18 Y 1-9 Follicular 2-11 Midcycle Luteal : 1-13 Post menopausal : Rejection criteria See Management of Laboratory Specimen (CL 7067);

47 MAGNESIUM Full : MAGNESIUM Short name: MG Blood Specimen - Serum - Plain tube (Red or yellow). - Plasma - Lithium heparin tube Routine : 8 Hrs STAT : 1 Hr Timed endpoint method Unit: mmol/l Age : Female & Male 0-1Y Y Y Y Y Y Rejection criteria See Management of Laboratory Specimen (CL 7067);

48 METHEMOGLOBIN Full : Methemoglobin Short name: MetHb /Analyzer Rejection criteria Whole blood collected in - Lithium heparin tube or - Blood gas capillary kit Should be in ice STAT / Routine: Sunday Sunday ( 24/7 Days ) STAT: 30 minutes Potentiometry,Amperometry,Conductance Unit: % upto 1.5 % of total Hb Wrong container / Clotted samples Samples not in ice Samples exceeded 60 minutes of collection

49 Micro-Albumin, Urine Full : Micro-Albumin, Urine Short name: MA Collect urine as 24hrs, timed, or random midstream sample (spot collection) in a clean collection container o C Routine: 8 Hrs Immunoturbidimetric Unit: mg/l Male / Female : Spot urine: 0 20 mg/l Timed urine: 0 30 mg/24hrs Albumin/Creatinine ratio: Male: Female: mg/mmol creatinine mg/mmol creatinine Rejection criteria See Management of Laboratory Specimen (CL 7067); - Dilution - Contamination

50 N TERMINAL PRO B-TYPE NATRIURETIC PEPTIDE Full : N terminal pro B-type natriuretic peptide Short name : NT-ProBNP Whole blood collected in EDTA tubes 2-3 ml blood in 4 ml tube only o C STAT: 1 hr Time resolved fluorometry(trf) Unit: pg/ml Male & Female < 249 Rejection criteria See Management of Laboratory Specimen (CL 7067) Clotted samples, Samples with unsuitable anticoagulant.

51 Parathyroid Hormone (Intact) Full : Parathyroid Hormone (Intact) Short name: PTH Blood Specimen: Serum - Serum - Plain tube (Red or yellow). Plasma - Lithium heparin tube 4 ml tube (Fill until the required mark) STAT / Routine: Sunday - Thursday Routine: 2 Days Chemiluminiscent Immunoassay Unit: pg/ml Rejection criteria See Management of Laboratory Specimen (CL 7067);

52 Phenobarbital Full : Phenobarbital, Phenobarbitone, Phenylethylmalonylurea, Barbita, Luminal. Short name: PHNO Blood Specimen: - Plain tube (red or yellow) - Lithium heparin tube 2-30 o C Routine: 8 Hrs STAT: 1 Hr Immunoturbidimetric Unit: μmol/l Male / Female : Rejection criteria See Management of Laboratory Specimen (CL 7067);

53 Phenytoin Full : Phenytoin, Epanutin, Dilantin, Diphenylan Sodium, Diphenylhydantoin Short name: PTN 2 ml blood in: -Plain tube (red or yellow) - Lithium heparin tube 2-30 o C Routine: 8 Hrs STAT: 1 Hr Immunoturbidimetric Unit: μmol/l. Male / Female : μmol/l. Rejection criteria See Management of Laboratory Specimen (CL 7067);

54 Phosphorus Full : PHOSPHORUS (Inorganic Phosphorus) Short name: PO4 Blood Specimen - Serum - Plain tube (red or yellow) - Plasma - Lithium heparin tube Routine: 8 Hrs STAT: 1 Hr Timed endpoint method (Phospho-Molybdate) Unit: mmol/l Age : Female : Male : 0 Mint- 1Y Y Y Y

55 14 20Y Y Rejection criteria See Management of Laboratory Specimen (CL 7067); Potassium Full : Potassium, Kalium Short name: K Blood Specimen - Serum - Plain tube ( red or yellow) - Plasma - Lithium heparin tube Routine: 8 Hrs STAT: 1 Hr ISE:INDIRECT Unit: mmol/l Age : Female & Male 0Mint 30D D 3Y Y

56 12-150Y Rejection criteria See Management of Laboratory Specimen (CL 7067); PROGESTERONE Full : PROGESTERONE Short name: PROG Blood Specimen - Serum - Plain tube ( red or yellow) Plasma-Lithium Heparin tube STAT / Routine: Sunday to Thursday Routine: 2 Days Chemiluminiscent Immunoassay Unit: nmol/l Female: <12 Y >12 Y Follicular Luteal 16 59

57 PMP < Rejection criteria See Management of Laboratory Specimen (CL 7067); PROLACTIN Full : PROLACTIN Short name: PROL Blood specimen: Serum - Plain tube (red or yellow ) Plasma - Lithium heparin tube 4 ml tube (Fill until the required mark) STAT / Routine: Sunday - Thursday Routine: 2 days Chemiluminiscent Immunoassay Unit: miu/l Age Female: Male: 0-2Y

58 2-5Y Y Y Y Rejection criteria See Management of Laboratory Specimen (CL 7067); PROSTATE SPECIFIC ANTIGEN (PSA) Full : PROSTATE SPECIFIC ANTIGEN (PSA) HORMON Short name: PSA Serum - Plain tube (red or yellow) Plasma - Lithium heparin tube 4 ml tube (Fill until the required mark) STAT / Routine: Sunday - Thursday Routine: 2 Days Immuno Enzymatic Assay Unit: ng/ml Age : Male:

59 Rejection criteria See Management of Laboratory Specimen (CL 7067); PROTEIN, Total Full : PROTEIN, Total Short name: TP Blood Specimen - Serum - Plain tube (red or yellow) - Plasma - Lithium heparin tube Routine: 8 Hrs STAT: 1 Hr Timed endpoint Biuret method Unit: g/l

60 Age : Female & Male < 1Y Y Y Y Y Rejection criteria See Management of Laboratory Specimen (CL 7067) Sodium Full : Sodium, Natrium Short name: NA Blood Specimen - Serum - Plain tube (red or yellow) - Plasma - Lithium heparin tube Routine: 8 Hrs STAT: 1 Hr ISE:INDIRECT Unit: mmol/l

61 Age : Female & Male 0Mint 150Y Rejection criteria See Management of Laboratory Specimen (CL 7067) TESTOSTERONE Full : TESTOSTERONE Short name: TESTOS Blood Specimen - Serum - Plain tube (red or yellow) 4 ml tube (Fill until the required mark) - Plasma - Lithium heparin tube STAT / Routine: Sunday - Thursday Routine: 2 Days

62 Chemiluminiscent Immunoassay Unit: nmol/l Male Female Rejection criteria See Management of Laboratory Specimen (CL 7067); THEOPHYLLIN Full : Theophylline; Aminophylline, Elixophylline, Slo-phylline, Sustaire, Theo-Dur, Theolair, Theospan. Short name: THEO Blood Specimen - Serum - Plain tube (red or yellow) - Plasma - Lithium heparin tube 2-30 o C Routine: 8 Hrs

63 STAT: 1 Hr Immunoturbidimetric Unit: umol/l Adult umol/l Neonatal Apnea umol/l Ped. Asthma umol/l Rejection criteria See Management of Laboratory Specimen (CL 7067) Thyroglobulin Full : Thyroglobulin Short name: Tg Blood Specimen - Serum - Plain tube (red or yellow) - Plasma - Lithium heparin tube

64 4 ml tube (Fill until the required mark) By batch - Every Thursday Routine: 8 Days Chemiluminiscent Immunoassay Unit: ng/ml Athyrotic < 0.1 Intact Thyroid < 33 Rejection criteria Note: Below comment will be attached to all Thyroglobulin outgoing reports Thyroglobulin (Tg) levels must be interpreted in the context of TSH levels, serial Tg measurements and radioiodine ablation status. Tg levels < 0.1 ng/ml in athyrotic individuals on suppressive therapy indicate a minimal risk (<1-2%) of clinically detectable recurrent papillary/follicular thyroid cancer. See Management of Laboratory Specimen (CL 7067); TSH Full : THYROID STIMULATING HORMONE Short name: TSH Serum - Plain tube (red or yellow) Plasma - Lithium heparin tube 4 ml tube (Fill until the required mark)

65 STAT / Routine: Sunday - Thursday Routine: 2 days /Analyzer Chemiluminiscent Immunoassay Unit: miu/l Male & Female: Age From Age To Sex Reference Range 0 years 12 years <12 years < 19 years Both Both years 150years Both For Pregnant : 1 st Trimester 2 nd Trimester 3 rd Trimester Female Female Female Rejection criteria See Management of Laboratory Specimen (CL 7067); Performing Lab Location TRANSFERRIN Full : TRANSFERRIN Short name: TRSF, TRF Blood Specimen - Serum - Plain tube (red or yellow)

66 - Plasma - Lithium heparin tube Routine: 8 Hrs STAT: 1 Hr Immunoturbidimetric Unit: g/l Age : Female & Male : 0 2 M Y Y Y Rejection criteria See Management of Laboratory Specimen (CL 7067). Triglycerides Full : Triglycerides, Triglycerols.

67 Short name: TG Blood Specimen - Serum - Plain tube (red or yellow) - Plasma - Lithium heparin tube Routine: 8 Hrs STAT: 1 Hr Timed end point method Unit: mmol/l Male & Female): < 1.70 Normal Borderline high High Rejection criteria See Management of Laboratory Specimen (CL 7067); TROPONIN I Full : Troponin I

68 Short name:trop I Whole blood collected in EDTA tubes 2-3 ml blood in 4 ml tube only o C STAT: 1 hr Rejection criteria Time resolved fluorometry(trf) Unit: ng/ml < 0.02 See Management of Laboratory Specimen (CL 7067); Clotted samples, Samples with unsuitable anticoagulant. TROPONIN T

69 Full : Troponin T Short name:trop T Whole blood collected in EDTA/Lithium heparin tubes 2-3 ml blood in 4 ml tube only o C STAT: 1 hr Rejection criteria Time resolved fluorometry(trf) Unit: ng/l 0-14 See Management of Laboratory Specimen (CL 7067); Clotted samples, Samples with unsuitable anticoagulant.

70 UREA/BUN Full : UREA / BLOOD UREA NITROGEN Short name: BUN Blood Specimen - Serum - Plain tube (red or yellow) - Plasma - Lithium heparin tube Routine: 8 Hrs STAT: 1 Hr Enzymatic Age : Female : Male : < 1Y Y Y Y Y Y Y Rejection criteria See Management of Laboratory Specimen (CL 7067);

71 Uric Acid Full : URIC ACID,URATE Short name: UA Blood Specimen - Serum - Plain tube (red or yellow) - Plasma - Lithium heparin tube Routine: 8 Hrs STAT: 1 Hr Timed endpoint method Unit: μmol/l Age : Female : Male : Y Rejection criteria See Management of Laboratory Specimen (CL 7067);

72 VALPROIC ACID Full : Depakine, Depakote, Sodium Valporate, Valproic Acid Short name: VALP Blood Specimen - Serum - Plain tube (red or yellow) - Plasma - Lithium heparin tube 2-30 o C Routine: 8 Hrs STAT: 1 Hr Immunoturbidimetric Unit: μmol/l Therapeutic Range Male / Female : μmol/l Rejection criteria See Management of Laboratory Specimen (CL 7067);

73 Vancomycin Collection time Therapeutic Range Full : Vancomycin Short name: Vanco Trough: Just prior to next dose Peak: (not recommended) 1 hour after the end of infusion Blood in: - Plain red tube or yellow - Lithium heparin tube 2-30 o C Routine: 8 Hrs STAT: 1 Hr Immunoturbidimetric Unit: μg/ml. Trough: μg/ml. Rejection criteria See Management of Laboratory Specimen (CL 7067);

74 VITAMIN B-12 Full : VITAMIN B-12 Short name: Vit.B12 Serum - Plain red tube or yellow SST (2 ml - 5 ml) Plasma - Lithium heparin tube 4 ml tube (Fill until the required mark) STAT / Routine: Sunday - Thursday Routine : 2 days Chemiluminiscent Immunoassay Unit: pmol/l Reference Value Female/Male Rejection criteria See Management of Laboratory Specimen (CL 7067);

75 Vitamin D Total Full : Vitamin D Total Short name: VIT.D Serum - Plain red tube or yellow SST (2 ml - 5 ml) Plasma - Lithium heparin tube 4 ml tube (Fill until the required mark) STAT / Routine: Sunday - Thursday Routine: 2 Days Chemiluminiscent Immunoassay Unit: ng/ml Severe deficiency <10 Reference Value Mild to Moderate deficiency Optimum values Rejection criteria Possible toxicity > 80 See Management of Laboratory Specimen (CL 7067);

76 Blood Gases Full : BLOOD Gases Short name: ABG,VBG Specimen requirements Arterial or Venous blood collected in heparin syringes Arterial or Venous blood in Lithium heparin tube (Minimum 200 ul in 1 Ml Syringe ) Transport Temperature Days test performed Room temperature within 10 minutes from collection time, not to exceed 30 minutes STAT:24/7, per 7 days a week Turnaround time ( TAT ) /Anal yzer Reference value STAT:30 minutes Potentiometry, Amperometry, Conductance Analyzer :ABL90 Flex ( Radiometer ) Specimen Parameter Type Age From Age To Sex Reference Range Arterial Blood 1 Day 2 Day All >2 Days All All PH Venous Blood All All All Capillary Blood 1 Day 2 Day All Arterial Blood 0 Day 5 day All PCO2(mmH 23 g) 6 day Months All >18 All 35 45

77 PO2 (mmhg) TCO2 (mmol/l) months Venous Blood All All All Capillary Blood 0 Day 5 day All Months All day 23 months >18 yrs All Arterial Blood 0 Day 1 day All Day >18 yrs All Venous Blood All All All Capillary Blood 0 Day 1 day All day >18 yrs All Arterial / 0 days 4 days All Venous / 5 days 12 Yrs All Capillary Blood > 13 yrs All BE (mmol/l) BE(Ecf) (mmol/l) Arterial / Venous / Capillary Blood Arterial / Venous / Capillary Blood All All All -2 2 All All All -2 2 Rejection criteria Performing Lab Location SO2 (%) Arterial / Venous / Capillary Blood See Management of Laboratory Specimen (CL 7067 ) Clotted samples All All All 95% 99%

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