Electron microscope observations cells in the cervix
|
|
- Leslie Randall
- 6 years ago
- Views:
Transcription
1 Electron microscope observations cells in the cervix on Langerhans M. S. YOUNES, M.D.* E. M. ROBERTSON, M.D., F.R.C.O.G. SERGIO A. BENCOSME, M.D., PH.D. Philadelphia, Pennsylvania, and Kingston, Ontario, Canada Clear cells, identical in fine structure to Langerhans cells of the human epidermis, occur in the intercellular spaces of the squamous epithelium of the cervix. There is an apparent increase in the number of Langerhans cells in the cervical epithelia from cases with carcinoma in situ. Langerhans cells of the cervix are frequently mistaken for lymphocytes or for epithelial cells that have undergone cytoplasmic shrinkage. Th.e proper identification of Langerhans cells and other clear cells in the cervical epithelium may eliminate some unnecessary confusiotl in the interpretation of cellular changes in neoplasia. I N T H E c 0 u R s E of an electron microscope study on the normal cervix and cervices with carcinoma in situ, we were impressed by the frequent observation of a certain type of clear cells lodged among the squam.ous epithelial cells of the ectocervix. Under the light microscope, these cells were distinguishable from the adjacent epithelial ceils by their relatively smaller but hyperchromatic nuclei and by their clear translucent cytoplasm. The latter appearance could be easily mistaken fo r that of a lymphocyte or an epithelial cell which has From the Department of Obstetrics and Gynecology of the Jeflerson Medical College, Philadelphia, and the Departments of Pathology and Obstetrics an,d Gynecology, Queen s University, Kingston. Supported by Research Grants T-422 from the American Cancer Society and CA from the National Cancer Institute. The earlier part of this work was supported by a Research Grant from the Ontario Cancer Treatment an,d Research Foundation. Presented in part at the Fifteemh Annual Meeting of The American Society of Cytology, October, 1967, Denver, Colorado. Present address: Jeferson Medical College, 1025 Walnut Street, Philadelphia, Pennsylvania undergone cytoplasmic shrinkage. Examination of a large number of electron microscopy sections showed that we were not dealing with a preparation artifact but with special cells identical in fine structure to the Langerhans cell of the human epidermis. In the skin, two types of clear cells are known to occur among the cells of the epidermis. These are the melanocytes and Langerhans cells. While melanocytes are known to produce melanin granules, the origin and function of Langerhans cells are still disputable subjects. l-l1 Both types of clear cells of the epidermis have been studied thoroughly by various light and electron microscopists interested in dermatologic pathology Review of the literature indicates that Langerhans cells have not been known to occur in the cervix. The purpose of this paper is to report on the observation of Langerhans cells in the squamous epithelium of the normal cervix and in cervices with carcinoma in situ. An account of the morphology of these cells will be given. Material and methods The observations to be reported are based on, the study of cervical tissues obtained from 397
2 12 padents with nomnna; cewices dnnd k0i patients with carcinoma in situ, as shown by light microscopy of either cone biopsy or hysterectomy specimens, Tissue blocks were raken from random sites from al 22 cervices and processed for electron microscopy. Small pieces of tissue were fixed in CauL field s fixative,23 dehydrated in a graded series of ethanol solutions, and embedded in Epon 812. z4 Thin sections were cut on Porter-Hum Mt.-2 or Mt-1 ultramicrotomes. Most sections were stained with uranyl acetate in methanop5 and then double stained with lead citrate.26 A few sections were stained with iridium trichloride as follows: the grids containing the sections were immersed in absolute acetone for 3 minutes, then stained with a 25 per cent solution of iridium trichloride for 30 minutes. The grids were rinsed briefly in methanol and then stained with uranyl acetate in methanol.z5 All sections were examined with the Hitachi Light microscopy ill parabin-eml?eddeo, lematoxylin and e&n-stained sections showed clear cells in both normal and in situ epithelia. Clear cells were characterized jy small hyperchromatic nuclei and a trans-?ucent cytoplasm which differentiated them r rom adjacent epithelial cells (Fig. 1) I Although some clear ceils could be identified as inflammatory cells, the nature of others could not be ascertained by light microscopy a?one. Cursory examination of histologic preparations indicated an increase in the number of clear cells in the sections from carcinoma in situ cases. Under the electron microscope, cells with fine structural features, identical to Langerhans cells of the epidermis, were found among the epithelial cells of cervices with Fig. 1. Squamous epithelium of the cervix lrom a patient with carcinoma m situ. Clear c&s ~(arrows) are characterized by small hyperchromatic nuclei and translucent cytoplasms. 8; Hematoxylin and eosin staining. )
3 Volume 102 Number S Langerhans cells in cervix 399 Fig. 21. Low-power micrograph showing a Langerhans cell among squamous epithelial cells of the cervix. The Langerhans cell has a nucleus (Nl) which is darker than that of adjacent epithelial cells (NZ), the cytoplasm of Langerhans cell contains characteristic granules (arrows), Details of the area within the insert are shown in Fig. 3. (~18,600.)
4 Fig. 3. Higher magnification of the insert in Fig. 2 showing a portion of the nucleus of a Langerhans cell (NJ and the cytoplasm containing the characteristic granules (arrows). In this plane of sectioning the granules appear rod-shaped and contain central linear densities. Ch41, cell membrane of Langeshans cell; CM2, cell membrane of an adjacent epithelial cell;.e#, cytoplasm of epithelial cell; M, mitochondria. (~70,000.) carcinoma in situ but less frequently in the tain ton&laments and is genera:ly 9i-- iaw epithelium of norma! cervices. The Langer- electron density (Figs. 3 and 4). There are hans cell has an idented nucleus which ap- no desmosomal attachments between the pears darker than that of adjacent epithelial plasma membranes of Langerhans cells and cells (Fig. 2). Unlike epithelial cells, the epithelial cells (Figs. 3 and 4). Langerhans cytoplasm of Langerhans cells does not con- cells contain the usual array of organelles
5 Langerhans cells in cervix 401 Fig. 4,. Details of structure of a cervical squamous epithelial cell. In contrast to Langerhans cells the cytoplasm of epithelial cells contains tonofilaments (Tf) and the cell is attached to adjacent epithelial cells by desmosomes CD). G, Golgi apparatus; Its, intercellular space; M, mitochondria; N, nucleus. (~31,000.) but, in addition, the cytoplasm shows granules which have been considered characteristic of Langerhans cells of the epidermis9 (Figs. 3 and 5). In the majority of sections these granules appear as rod-shaped bodies which may be attached to terminal vacuoles (Fig. 3). These granules may exhibit different appearances, depending upon the plane of sectioning. The characteristic Langerhans cells granule has been variously described as rod-shaped,27 disk-shaped,le or racket-shaped.5 Recently, serial construction of these granules by Sagebiel and Reedz8 indicated thalt they are made of flattened or curved orthogonal net of particles bounded externally by a limiting membrane and that the granule may be disk-shaped, cup-shaped, or a composite of both shapes. Comment The present work has shown that Langerhans ceils are found in the intercelluiar spaces of the squamous epithelium of the cervix. While this may represent a new finding in the cervix, the presence of Langerhans cells in the epidermal cells of the human skin has long been recognized. Most of the literature dealing with theories on the origin and function of Langerhans cells was concerned with the human epidermis. It is beyond the scope of this paper to discuss in detail all of these theories. Briefly, Langerhans cells have been considered precursors of melanocytes,l postmelanin synthetic cells,5 intraepidermal nerve endings,6 or effete melanocytes.4 It is evident that most of these theories have attempted to either prove or disprove a correlation between Langerhans cells and melanocytes of the epidermis. The application of these theories to the cervix is of very little value, since the latter organ is not known to be the site of active melanogenesis. Recently, the demonstration of the characteristic Langer-
6 a
7 Volume 102 Number 3 Langerhans cells in cervix 403 ham cell granule in a histiocytosis X lesion of the lung and eosinophilic granuloma of bone has cast doubt on the specificity of this type of granule. 28 In brief, the function of Langerhans cells in the cervical m.ucosa remains obscure. It is perhaps worthwhile to mention one of the earlier and widely accepted theories9 dealing with the origin of Langerhans cells. This theory suggests that active melanocytes are found only among the basal epidermal cells and that after completion of their function of melanogenesis they migrate toward the surface, appear as Langerhans cells, and are eventually exfoliated with epithelial ceils.g This theory may be of some significance when applied to the cervix, since the presence of Langerhans cells among exfoliated epithelial cells may cause some unnecessary confusion in the interpretation of cellular changes in neoplasia. The apparent increase in the number of Langerhans cells in carcinoma in situ remains unexplained. In the human skin, increased epidermal cell turnover is associated with a significant increase in the number of Langerhans cells.lg Mishimalg has shown that keratin-stripping of the skin leads to an increased epithelial cell turnover and a subsequent increase in the number of Langerhans cells. It is possible that the observed increase in the number of Langerhans cells in carcinoma in situ may be associated with the abnormal epithelial turnover in this lesion. The nature and function of Langerhans cells and other clear cells in the cervical mucosa in normal and pathologic conditions await further investigation REFERENCES Breathnach, A. S., and Goodwin, E. P.: J. Anat. (London) 99: 377, Wiedmann, A.: Hautarzt 15: 13, Bocharov, I. S.: Dokl. Bolg. Akad. Nauk. 199: 667, Billingham, R. E., and Medawar,.P. B.: Phil. Tr. Roy. Sot. (B) 237: 151, Zelickson, A. S.: J. Invest. Dermat. 44: 201, Ferreira-Marques, J.: Arch. Dermat. u. Syph. 193: 191, Breathnach, A. S.: J. Invest. Dermat. 40: 279, Richter, R.: Arch. Klin. u. Exper. Dermat. 202: Birbeck, M. S., Breathnach, A,. S., and Everall, J. D.: J. Invest. Dermat. 37: 51, Breathnach, A. S., Birbeck, M. S., and Everall, J. D.: Brit. J. Dermat. 74: 242, Breathnach, A. S., Birbeck, M. S., and Everall, J. D.: Ann. New York Acad. SC. 100: 223, Allan, A. C.: Ann. New York Acad. SC. 100: 4.4, Barnicot, N. A., and Birbeck, M. S.: In Montana and Ellis, editors: the Biology of Hair Growth, New York, 1958, Academic Press, Inc Birbeck, M. S. C.: Ann. New York Acad. SC. 108: 540, Breathnach, A. S.: J. Anat. (London) 98: 265, Breathnach, A. S., and Wyllie, L. M.: J. Invest. Dermat. 44: 60, Charles, A., and Ingram, J. T.: J. Biophys. & Biochem. Cytol. 6: 41, Langerhans, P.: Virchow Arch. path. Anat. 44: 325, Mishima, Y.: J. Cell Biol. 30: 417, Silvers, W. K.: Am. J. Anat. 100: 225, Zelickson, A. S., and Hartmann, J. F.: J. Invest. Dermat. 36: 23, Zelickson. A. S.: In Curtis. Arthur Cl.. editor: Electron Microscopy of Skin and Mucous Membrane, Springfield, Illinois, 1963, Charles C Thomas, Publisher. Caulfield, J. B.: J. Biophys. & Biochem. Cytol. 3: 827, Luft, J. H.: J. Biophys. & Biochem. Cytol. 9: 409, Stempak, J. G., and Ward, R. T.: J. Cell Biol. 22: 697, Venable, J. H., and Coggeshall, R.: J. Cell Biol. 25: 407, Zelickson, A. S.: J. Invest. Dermat. 47: 498, Sagebiel, R., and Reed, T. H.: J. Cell Biol. 36: 595, 1968.
CELLS CONTAINING LANGERHANS GRANULES IN HUMAN LYMPH NODES OF DERMATOPATHIC LYMPHADENOPATHY*
THS JOURNAL OF INVEBTIOATIVR DERMATOLOGY Copyright 1969 by The Williams & Wilkins Co. Vol. 93, No. 4 Printed in U.S.A. CELLS CONTAINING LANGERHANS GRANULES IN HUMAN LYMPH NODES OF DERMATOPATHIC LYMPHADENOPATHY*
More information:1c.c :& Preliminary and Short Report GRANULE FORMATION IN THE LANGERHANS CELL* structure with rounded ends and a striated lamella
THE JOURNAL OF INVESTIGATIVE DERMATOLOGY Copyright 1566 by The Williams & Wilkins Co. Vol. 7, No. 5 Printed in U.S.A. Preliminary and Short Report GRANULE FORMATION IN THE LANGERHANS CELL* ALVIN S. ZELICKSON,
More informationON THE PRESENCE OF A CILIATED COLUMNAR EPITHELIAL CELL TYPE WITHIN THE BOVINE CERVICAL MUCOSA 1
ON THE PRESENCE OF A CILIATED COLUMNAR EPITHELIAL CELL TYPE WITHIN THE BOVINE CERVICAL MUCOSA 1 R. I. Wordinger, 2 J. B. Ramsey, I. F. Dickey and I. R. Hill, Jr. Clemson University, Clemson, South Carolina
More informationELECTRON MICROSCOPIC STUDY OF MELANIN-PHAGOCYTOSIS BY CUTANEOUS VESSELS IN CELLULAR BLUE NEVUS*
THE JOURNAL 05' INVESTIGATIVE DERMATOLOGY Copyright 1969 by The Williams & Wilkinl Co. Vol. 62, No. 6 Printed in U.S.A. ELECTRON MICROSCOPIC STUDY OF MELANIN-PHAGOCYTOSIS BY CUTANEOUS VESSELS IN CELLULAR
More informationTRANSFER OF PREMELANOSOMES INTO THE KERATINIZING CELLS OF ALBINO HAIR FOLLICLE
TRANSFER OF PREMELANOSOMES INTO THE KERATINIZING CELLS OF ALBINO HAIR FOLLICLE PAUL F. PARAKKAL. From the Department of Dermatology, Boston University School of Medicine, Boston, Massachusetts 02118 INTRODUCTION
More informationthe structure of their ducts has been
Tza JOURNAL 0? INVEa'riGATrVN DEBMATOLOOT Copyright t 1966 by The Williams & Wilkins Co. Vol. 46, No. I Printed in U.S.A. AN ELECTRON MICROSCOPIC STUDY OF THE ADULT HUMAN APOCRINE DUCT* KEN HASHIMOTO,
More informationHistopathology: Cervical HPV and neoplasia
Histopathology: Cervical HPV and neoplasia These presentations are to help you identify basic histopathological features. They do not contain the additional factual information that you need to learn about
More informationtt 't'.swrt. I i LOCALIZATION OF GOLD CHLORIDE AND ADENOSINE TRIPHOSPHATASE IN HUMAN LANGERHANS CELLS*
THE JOURNAL OF INVESTIGATIVE DERMATOLOGY Copyright 1968 by The Williams & Wilkins Co. Vol. 51, No. 5 Printed in U.S.A. LOCALIZATION OF GOLD CHLORIDE AND ADENOSINE TRIPHOSPHATASE IN HUMAN LANGERHANS CELLS*
More informationELECTRON MICROSCOPY OF A SMALL PIGMENTED CUTANEOUS LESION*
ELECTRON MICROSCOPY OF A SMALL PIGMENTED CUTANEOUS LESION* The description of the lesion in the title of this rcport is intentionally non-committal. Diagnosed clinically as a lentigo, it was removed as
More informationAN ELECTRON MICROSCOPE STUDY OF BASAL MELANOCYTES AND HIGH-LEVEL CLEAR CELLS (LANGERHANS CELLS) IN VITILIGO*
AN ELECTRON MICROSCOPE STUDY OF BASAL MELANOCYTES AND HIGH-LEVEL CLEAR CELLS (LANGERHANS CELLS) IN VITILIGO* MICHAEL S. BIRBECK, MAt, AODAN S. BREATHNACH, M.Sc., M.D.t AND JOHN D. EVERALL, M.R.C.P. Vitiligo
More informationSome Observations on the Fine Structure of the Goblet Cells. Special Reference to the Well-Developed Agranular Endoplasmic Reticulum
Okajimas Folia Anat. Jpn., 58(4-6) : 583-594, March 1982 Some Observations on the Fine Structure of the Goblet Cells in the Nasal Respiratory Epithelium of the Rat, with Special Reference to the Well-Developed
More informationSilver-Impregnation of the Golgi Complex in Epididymal Epithelial Cells of Mice
CELL STRUCTURE AND FUNCTION 8, 339-346 (1984) C by Japan Society for Cell Biology Silver-Impregnation of the Golgi Complex in Epididymal Epithelial Cells of Mice Ikuo Yamaoka, Sumie Katsuta and Yoshimi
More informationR,;habdomyosarcoma, the most common
Fine-structural classification of orbital rhabdomyosarcoma Arnold J. Kroll Six cases of orbital rhabdomyosarcoma were studied with the electron microscope. Tumor cells (rhabdomyoblasts) could be classified
More informationfact that the origin and fate of these subcellular shown that they may be found in cells of
THE JOURNAL OF INVESTIGATIVE DEHMATOLOGY Copyright 1569 by The Williams & Wilkins Co. vol. 52, No. 5 Printed in U.S.A. INTRANIJCLEAR AND EXTRACELLIJLAR LANGERHANS' CELL GRANTJLES* KLAUS WOLFF, M.D. Acm
More informationNew aspect of hepatic nuclear glycogenosis
J. clin. Path. (1968), 21, 19 New aspect of hepatic nuclear glycogenosis in diabetes1 F. CARAMIA, F. G. GHERGO, C. BRANCIARI, AND G. MENGHINI From the Institute of General Pathology, University of Rome,
More informationELECTRON MICROSCOPIC STUDIES ON EQUINE ENCEPHALOSIS VIRUS
Onderstepoort]. vet. Res. 40 (2), 53-58 (1973) ELECTRON MICROSCOPIC STUDIES ON EQUINE ENCEPHALOSIS VIRUS G. LECATSAS, B. J. ERASMUS and H. J. ELS, Veterinary Research Institute, Onderstepoort ABSTRACT
More informationHistopathology: skin pathology
Histopathology: skin pathology These presentations are to help you identify, and to test yourself on identifying, basic histopathological features. They do not contain the additional factual information
More informationDifferentially Localized Incorporation of Amino Acids. in Relation to Epidermal Keratinization in the Newborn Rat '
Differentially Localized Incorporation of Amino Acids - in Relation to Epidermal Keratinization in the Newborn Rat ' KIMIE FUKUYAMA, TOSHIO NAKAMURA AND I. A. BERNSTEIN Department of Dermutology, Tokyo
More informationElectron Microscope Studies of the Human Epidermis The Clear Cell of Masson (Dendritic Cell or Melanocyte)*
Published Online: 25 November, 1958 Supp Info: http://doi.org/10.1083/jcb.4.6.679 Downloaded from jcb.rupress.org on July 1, 2018 Electron Microscope Studies of the Human Epidermis The Clear Cell of Masson
More informationEpidermis. Integumentary system
Epidermis the doctor mentioned at the begging of the lecture that the slides is from different sources and has information and details that is enough for us so we don t have to go back and read from the
More informationMansoura university Faculty of medicine Histology and cell Biology Department Curriculum Content And Logbook
Mansoura university Faculty of medicine Histology and cell Biology Department Curriculum Content And Logbook For the 1 st year Medical Student s In Histology and cell Biology Mansoura university Faculty
More informationSkin. Kristine Krafts, M.D.
Skin Kristine Krafts, M.D. Skin Lecture Objectives Describe the functions of skin. Describe the structure, location and function of the cell types found in epidermis: keratinocytes, melanocytes, Langerhans
More informationThe Extracellular Nature of Enamel in the Rat*
Published Online: 1 June, 1960 Supp Info: http://doi.org/10.1083/jcb.7.3.489 Downloaded from jcb.rupress.org on September 1, 2018 The Extracellular Nature of Enamel in the Rat* By MICHAEL L. WATSON, Ph.D.
More informationand biochemical studies have defined and clarified many problems in normal and aberrant cutaneous pigmentation (1 14).
THE JOURNAL OF IRTESTIOATIVE DERMATOLOGY Copyright 1065 by The Williams & Wilkins Co. Vol. 50, No. 6 Prirtted ir U.S.A. TJLTRASTRUCTURE OF HALO NEVI* JOHN L. SWANSON, CAPTAIN, MC, USAR, DONALD M. WAYTE,
More informationSOME ESSENTIAL FACTORS IN THE PATHOLOGY AND TREATMENT OF CANCER OF THE SKIN LOUIS H. JORSTAD, M.D.
SOME ESSENTIAL FACTORS IN THE PATHOLOGY AND TREATMENT OF CANCER OF THE SKIN LOUIS H. JORSTAD, M.D. (From the Department of Pathology, the Barnard Free Skin and Cancer Hospital, St. Louis, Missouri) The
More informationChanges of organelles associated with the differentiation of epidermal melanocytes in the mouse
/. Embryol. exp. Morph. Vol. 43, pp. 107-121, 197S ]Ç)J Printed in Great Britain Company of Biologists Limited 1978 Changes of organelles associated with the differentiation of epidermal melanocytes in
More informationELECTRON MICROSCOPIC STUDY OF THE FORMATION OF BLUETONGUE VIRUS*
Onderstepoort J. vet. Res. (1968), 35 (1), 139-150 Printed in the Repub. of S. Afr. by The Government Printer, Pretoria ELECTRON MICROSCOPIC STUDY OF THE FORMATION OF BLUETONGUE VIRUS* G. LECATSAS, Veterinary
More informationOat-cell carcinoma of the oesophagus: a recently recognized entity
J. clin. Path., 1976, 29, 1068-1073 Oat-cell carcinoma of the oesophagus: a recently recognized entity M. G. COOK, V. EUSEBI, AND C. M. BETTS From the Institute ofpathology, The London Hospital, London
More informationSkin (Integumentary System) Wheater, Chap. 9
Skin (Integumentary System) Wheater, Chap. 9 Skin (Integument) Consists of skin and associated derivatives Largest organ of body (21 ft 2 ; 9 lbs.; has 11 miles of blood vessels) Functions: Protection
More informationThe Fine Structure of the Epithelial Cells of the Mouse Prostate* II. Ventral Lobe Epithelium
Published Online: 1 June, 1960 Supp Info: http://doi.org/10.1083/jcb.7.3.511 Downloaded from jcb.rupress.org on September 28, 2018 The Fine Structure of the Epithelial Cells of the Mouse Prostate* II.
More informationElectron Microscopy of Small Cells: Mycoplasma hominis
JOURNAL of BAcTRiowOY, Dc. 1969, p. 1402-1408 Copyright 0 1969 American Society for Microbiology Vol. 100, No. 3 Printed In U.S.A. NOTES Electron Microscopy of Small Cells: Mycoplasma hominis JACK MANILOFF
More informationIdentification of the spermatogenic stages in living seminiferous tubules of man
Identification of the spermatogenic stages in living seminiferous tubules of man V. Nikkanen, K.-O. S\l=o"\derstr\l=o"\m and M. Parvinen Department of Obstetrics and Gynecology, Turku University Central
More informationFine Structure of the Normal Trigeminal Ganglion in the Cat and Monkey*
Fine Structure of the Normal Trigeminal Ganglion in the Cat and Monkey* DAVID S. MAXWELL, PH.D. Principal Contributor and Leader of Discussion HE inclusion of animal material m a y be justified as a means
More information(From The Rockefeller Institute) Materials and Methods. Observations with the Electron Microscope
ELECTRON MICROSCOPE STUDY OF THE DEVELOPMENT OF THE PAPILLOMA VIRUS IN THE SKIN OF THE RABBIT* BY ROBERT S. STONE,~ M.D., RICHARD E. SHOPE, M.D., DAN H. MOORE, P,~.D. (From The Rockefeller Institute) PLATES
More informationDr. Issraa Ali Hussein
CLINICAL 09888888;rCYTOLOGY Dr. Issraa Ali Hussein objectives Define diagnostic cytology (clinical cytology). Explain the differences between histopathology and cytopathology. Recognize the methods for
More informationTHE FORM OF HAEMOGLOBIN IN THE ERYTHROCYTES OF THE COD, GADUS CALLARIAS
J. Cell Set. 8, 407-412 (1971) 407 Printed in Great Britain THE FORM OF HAEMOGLOBIN IN THE ERYTHROCYTES OF THE COD, GADUS CALLARIAS N.W.THOMAS Department of Anatomy, Marischal College, Aberdeen, Scotland
More informationAN ULTRASTRUCTURA-L STUDY OF THE MEMBRANES OF KERATINIZING WOOL FOLLICLE CELLS
J. Cell Sci. II, 205-219 (1972) 205 Printed in Great Britain AN ULTRASTRUCTURA-L STUDY OF THE MEMBRANES OF KERATINIZING WOOL FOLLICLE CELLS D.F.G.ORWIN AND R.W.THOMSON Wool Research Organisation of N.Z.
More informationTitle. Author(s)SUGIMURA, Makoto; YAMADA, Junzo. CitationJapanese Journal of Veterinary Research, 18(1): Issue Date DOI. Doc URL.
Title BURSA OF FABRICIUS CONTAINING VIRUS-LIKE PARTICLES A OBSERVATION Author(s)SUGIMURA, Makoto; YAMADA, Junzo CitationJapanese Journal of Veterinary Research, 18(1): 31-3 Issue Date 1970-03 DOI 10.14943/jjvr.18.1.31
More informationDifferentiation of Renal Tubular Epithelium in Renal Transplantation Cytology
Differentiation of Renal Tubular Epithelium in Renal Transplantation Cytology G. BERRY SCHUMANN, M.D., LAWRENCE J. PALMIERI, B.S., C.T.(ASCP), AND DAVID B. JONES, M.D. Schumann, G. Berry, Palmieri, Lawrence
More informationupper part of the hair follicle (above the
THE JOURNAL OF INVESTIOATIYE DERMATOLOOY Copyright 1967 by The Williams & Wilkins Co. Vol. 48, No. 4 Printed in U.S.A. AN ULTRASTRUCTURE STUDY OF THE EXPERIMENTAL KERATOACANTHOMA* In recent years, the
More informationCOMPARATIVE DISTRIBUTION OF CARBOHYDRATES AND LIPID DROPLETS IN THE GOLGI APPARATUS OF INTESTINAL ABSORPTIVE CELLS
COMPARATIVE DISTRIBUTION OF CARBOHYDRATES AND LIPID DROPLETS IN THE GOLGI APPARATUS OF INTESTINAL ABSORPTIVE CELLS JEAN A. SAGE and RALPH A. JERSILD, JR. Medical Center, Indianapolis, Indiana 46202 From
More informationUltrastructure of malignant melanomas of the choroid
Ultrastructure of malignant melanomas of the choroid Michael J. Hogan and Lynette Feeney Eleven vialignant melanomas of the uveal tract have been studied by light and electron microscopy. Two types of
More informationTissues. Tissues - Overview. Bio 101 Laboratory 3. Epithelial Tissues and Integument
Bio 101 Laboratory 3 Epithelial Tissues and Integument 1 Tissues Tissues to be examined under the microscope Epithelial Tissue Integument Connective Tissue **We will be doing muscle and nervous tissues
More informationPublished Online: 25 November, 1956 Supp Info: on November 16, 2018 jcb.rupress.org Downloaded from
Published Online: 25 November, 1956 Supp Info: http://doi.org/10.1083/jcb.2.6.799 Downloaded from jcb.rupress.org on November 16, 2018 B~IEF NOrmS 799 Permanganate--A New Fixative for Electron Microscopy.*
More informationPhagocytic activity of the pigmented retinal epithelium. III. Interaction between lysosomes and ingested polystyrene spheres*
Phagocytic activity of the pigmented retinal epithelium. III. Interaction between lysosomes and ingested polystyrene spheres* Joe G. Hollyfield and Adriana Ward Outer segment fragments discarded by rod
More informationMedical School Histology Basics Introduction to Microscopy. VIBS 289 lab
Medical School Histology Basics Introduction to Microscopy VIBS 289 lab Larry Johnson Texas A&M University Objectives Learn the difference in magnification and resolution Learn about different types of
More informationInterpretation guide. Abnormal cytology can t hide anymore
Interpretation guide Abnormal cytology can t hide anymore Unique dual-biomarker technology makes you certain about the presence of transforming HPV infection. The science that creates certainty. Table
More informationEpithelia will be discussed according to the following scheme: Type Number of layers Shape Line drawing. Squamous Cuboidal Columnar
Epithelia Epithelia will be discussed according to the following scheme: Type Number of layers Shape Line drawing Simple Squamous Cuboidal Columnar Covering and Lining epithelium Pseudostratified Stratified
More informationChapter 5: The Integumentary System - Introduction and Epidermis
Chapter 5: The Integumentary System - Introduction and Epidermis The Integument Means Covering Composed: Skin Hair Nails Sweat glands Oil glands The Integument Thickness 1.5 4 mm (or more) Weight 9 11
More informationOF THE HUMAN ORAL MUCOSA (FORDYCE'S DISEASE)*
THE JOURNAL OF INVESTIGATIVE DERMATOLOGY Copyright 1969 by The Williams & Wilkins Co. VoL 53, No.3 Fri nied in U.S.A. TJLTRASTRUCTURAL OBSERVATIONS ON SEBACEOUS GLANDS OF THE HUMAN ORAL MUCOSA (FORDYCE'S
More informationINTRAEPITHELIAL MAST CELLS IN GINGIVAL LICHEN PLANUS: AN ULTRASTRUCTURAL STUDY
THE JOURNAL OF INVESTIGATIVE DERMATOLOTY, 64:436-440, 1975 Copyright 1975 by The Williams & Wilkins Co. Vol. 64, No.6 Printed in U.S.A. INTRAEPITHELIAL MAST CELLS IN GINGIVAL LICHEN PLANUS: AN ULTRASTRUCTURAL
More informationELECTRON MICROSCOPE STUDY OF HUMAN WARTS; SITES OF VIRUS PRODUCTION AND NATURE OF THE INCLUSION
ELECTRON MICROSCOPE STUDY OF HUMAN WARTS; SITES OF VIRUS PRODUCTION AND NATURE OF THE INCLUSION BODIES * JUNE D. ALMEIDA, ALLAN F. HOWATSON, Pn.D. AND MURRAY G. WILLIAMS, M.D. The viral etiology of human
More informationRelationship of Ehrlichia canis-infected Mononuclear Cells to Blood Vessels of Lungs1
INFECTION AND IMMUNITY, Sept. 1974, p. 590-596 Copyright 0 1974 American Society for Microbiology Vol. 10, No. 3 Printed in U.S.A. Relationship of Ehrlichia canis-infected Mononuclear Cells to Blood Vessels
More informationReceived, June 29, 1904; accepted for publication
THE AMEBICAN JOURNAL OF CLINICAL PATHOLOGY Copyright 1964 by The Williams & Wilkins Co. Vol. 42, No. 0 Printed in U.S.A. CARCINOMA IN SITU OF THE ENDOMETRIUM ISABELLE A. BUEHL, M.D., PRANK VELLIOS, M.D.,
More informationCINtec p16 INK4a Staining Atlas
CINtec p16 INK4a Staining Atlas Rating Rating Positive The rating positive will be assigned if the p16 INK4a -stained slide shows a continuous staining of cells of the basal and parabasal cell layers of
More informationNormal Morphology. Anatomic Considerations. Normal Urothelial Histology and Cytology
1 Normal Morphology Anatomic Considerations The urinary tract can be divided into three regions: the kidney; the calyces, pelves and ureters (upper collecting system or upper tract); and the bladder and
More informationSQUAMOUS CELLS: Atypical squamous cells (ASC) - of undetermined significance (ASC-US) - cannot exclude HSIL (ASC-H)
SQUAMOUS CELLS: Atypical squamous cells (ASC) - of undetermined significance (ASC-US) - cannot exclude HSIL (ASC-H) ASC refers to cytologic changes suggestive of SIL, which are qualitativley or quantitatively
More informationElectron Microscopical Findings in Hypertrophied Human Ventricle
Brit. Heart J., 1969, 31, 200. Electron Microscopical Findings in Hypertrophied Human Ventricle K. DOWLATSHAHI AND A. C. HUNT From the Cardiac Unit, Royal Infirmary, Bristol, and the Department of Pathology,
More information(Plates LXVIII-LXXI)
[GANN, 54, 481-486; December, 1963] UDC 616.155.392-076.4:578.69 VIRUS-LIKE PARTICLES IN HUMAN CHLOROLEUKEMIA CELLS (Plates LXVIII-LXXI) Zensuke OTA, Shin-ya SUZUKI, and Satoru HIGASHI (Department of Internal
More informationCells in Peripheral Leg Lymph of Normal Men
202 Lymphology 11 (1978) 202-207 Summary Cells in Peripheral Leg Lymph of Normal Men J. Sokolowski, E. Jakobsen, J.V. Johannessen Laboratory of Hematology and Lymphology and Laboratory for Ultrastructural
More informationIT has been shown (Chou, 1957 a, b) that there are three kinds of lipid
279 The Ultra-fine Structure of Lipid Globules in the Neurones of Helix aspersa By J. T. Y. CHOU and G. A. MEEK (From the Cytological Laboratory, Department of Zoology; and Department of Human Anatomy,
More informationMELANOCYTE PATTERN OF AN AREA OF FRECKLED EPIDERMIS COVERING A STRETCHED SCAR*
MELANOCYTE PATTERN OF AN AREA OF FRECKLED EPIDERMIS COVERING A STRETCHED SCAR* Figure 1 illustrates the appearance after eighteen months of the scar resulting from the removal of an area of full thickness
More informationChapter 4 :Organization & Regulation of Body Systems
Chapter 4 :Organization & Regulation of Body Systems 4.1 Types of tissues What is a tissue? A collection of cells of the same type that perform a common function There are 4 major tissue types in the body:
More informationUterine Cervix. Protocol applies to all invasive carcinomas of the cervix.
Uterine Cervix Protocol applies to all invasive carcinomas of the cervix. Protocol revision date: January 2005 Based on AJCC/UICC TNM, 6 th edition and FIGO 2001 Annual Report Procedures Cytology (No Accompanying
More informationAN ATTEMPT TO INDUCE "PIGMENT SPREAD" IN FRECKLED HUMAN SKIN*
AN ATTEMPT TO INDUCE "PIGMENT SPREAD" IN FRECKLED HUMAN SKIN* White skin transplanted on to a pigmented area of a spotted black-and-white guinea-pig becomes blackened, and black skin transplanted on to
More informationTHE CLASSIFICATION OF BLADDER TUMOURS
41 THE CLASSIFICATION OF BLADDER TUMOURS T. J. DEELEY AND V. J. DESMET* From the Radiotherapy Department, Hammersmith Hospital, Du Cane Road, London, IF7.12, and the Department of Pathology, Louvain University,
More informationDistribution of the Pores of Epithelial Basement Membrane in the Rat Small Intestine
FULL PAPER Anatomy Distribution of the Pores of Epithelial Basement Membrane in the Rat Small Intestine Takashi TAKEUCHI 1) and Tatsuo GONDA 1) 1) Institute of Experimental Animals, Shimane Medical University,
More informationThe Ultrastructure of the Chloroplast in Spinacia oleracea
1964 415 The Ultrastructure of the Chloroplast in Spinacia oleracea Katsumi Ueda Botanical Institute, Nara Women's University, Nara, Japan Received March 16, 1964 Studies of the ultrastructure of the chloroplasts
More informationUltrastructure of Connective Tissue Cells of Giant African Snails Achatina fulica (Bowdich)
Kasetsart J. (Nat. Sci.) 36 : 285-290 (2002) Ultrastructure of Connective Tissue Cells of Giant African Snails Achatina fulica (Bowdich) Viyada Seehabutr ABSTRACT The connective tissue sheath of cerebral
More informationSTUDIES OF THE HUMAN UNFERTILIZED TUBAL OVUM*t
FERTILITY AND STERILITY Copyright @ 1973 by The Williams & Wilkins Co. Vol. 24, No.8, August 1973 Printed in U.S.A. STUDIES OF THE HUMAN UNFERTILIZED TUBAL OVUM*t C. NORIEGA, M.D., AND C. OBERTI, M.D.
More informationThe Endocrine Cells of the Upper Gut Mucosa in Dogs with Transplantation of the Pyloric Antrum to the Colon*
Z. Zellforsch. 146, 237--242 (1973) by Springer-Verlag 1973 The Endocrine Cells of the Upper Gut Mucosa in Dogs with Transplantation of the Pyloric Antrum to the Colon* Juan Lechago and Sergio A. Bencosme
More informationDURING the past ten years it has been suggested that the classical form
163 The Golgi Apparatus and Lipoidal Bodies in Exocrine and Endocrine Cells in the Pancreas of Man By DENNIS LACY (From the Department of Zoology and Comparative Anatomy, St. Bartholomew's Medical College)
More informationTHE QUESTION OF RELATIONSHIP BETWEEN GOLGI VESICLES AND SYNAPTIC VESICLES IN OCTOPUS NEURONS
J. Cell Set. 7, 89- (97) Printed in Great Britain THE QUESTION OF RELATIONSHIP BETWEEN GOLGI VESICLES AND SYNAPTIC VESICLES IN OCTOPUS NEURONS E. G. GRAY Department of Anatomy, University College London,
More informationLecture Overview. Marieb s Human Anatomy and Physiology. Chapter 4 Tissues: The Living Fabric Epithelial Tissues Lecture 9. Introduction to Tissues
Marieb s Human Anatomy and Physiology Marieb Hoehn Chapter 4 Tissues: The Living Fabric Epithelial Tissues Lecture 9 Lecture Overview Introduction to Tissues Epithelial Tissues Location General characteristics
More informationThe application of x-ray diffraction and electron microscopy to the study of human peripheral
J. clin. Path. (1961), 14, 152. The application of x-ray diffraction and electron microscopy to the study of human peripheral nerve obtained by biopsy J. B. FINEAN AND A. L. WOOLF From the Department of
More informationwith particular emphasis on the vacuolar
Tea JOURNAL OF TNvasTJoATrvg DERMAT0L00Y Copyright. 1959 by The Williams & Wilkins Co. vol. 52, No.2 Printed in U.S.A. TJLTRASTRUCTTJRAL PATHOLOGY OF ERYTHEMA DYSCHROMICUM PERSTANS* NICHOLAS A. SOTER,
More informationIntegumentary System. Integumentary System
1. General aspects a. The integumentary system consists of several organs major organ of the system is the skin other organs are relatively small and they can be considered as specialized structures of
More informationActinic keratosis (AK): Dr Sarma s simple guide
Actinic keratosis (AK): Dr Sarma s simple guide Actinic keratosis is a very common lesion that you will see in your day-to-day practice. First, let me explain the name Actinic keratosis. It means keratosis
More informationHISTOLOGY VIRTUAL LABORATORY BLOOD AND LYMPHATICS SYSTEM
HISTOLOGY VIRTUAL LABORATORY BLOOD AND LYMPHATICS SYSTEM Login: http://histopath.westernu.edu Histology Atlas AND Virtual Histology links. I. HEMATOLOGY - PERIPHERAL BLOOD Purpose: To be able to identify
More informationwas no relation between the presence of oropharyngeal symptoms and the degree of anaemia,
J. clin. Path. (1960), 13, 463. THE BUCCAL MUCOSA IN BY From Paddington General Hospital, Lonidon (RECEIVED FOR PUBLICATION MAY 10, 1960) ANAEMIA The histological features of the buccal mucosa have been
More informationElectron Microscope Studies of the Human Epidermis The Cell Boundaries and Topography of the Stratum Malpighii*
Electron Microscope Studies of the Human Epidermis The Cell Boundaries and Topography of the Stratum Malpighii* By RICHARD G. HIBBS, Ph.D., and WALLACE H. CLARK, Jr.,~: M.D. With the Technical Assistance
More informationTHE INFLUENCE OF ULTRAVIOLET LIGHT ON THE LANGER-
THE JOURNAL OF INVESTIGATIVE DERMATOLOGY Copyright 167 by The Williams & Wilkins Co. Vol. 48, No. 6 Printed in C.S.II. THE INFLUENCE OF ULTRAVIOLET LIGHT ON THE LANGER- HANS CELL POPULATION AND ITS HYDROLYTIC
More informationENHANCEMENT OF THE GRANULATION OF ADRFNERGIC STORAGE VESICLES IN DRUG-FREE SOLUTION
ENHANCEMENT OF THE GRANULATION OF ADRFNERGIC STORAGE VESICLES IN DRUG-FREE SOLUTION TAKASHI IWAYAMA and J. B. FURNESS. From the Department of Zoology, University of Melbourne, Victoria, Australia. Dr.
More informationHistology of Skin. dr. Alya Amila Fitrie, M.Kes dr. Radita Nur Anggraeni Department of Histology Medical Faculty University of North Sumatera 2008
Histology of Skin dr. Alya Amila Fitrie, M.Kes dr. Radita Nur Anggraeni Department of Histology Medical Faculty University of North Sumatera 2008 1 INTRODUCTION The heaviest single organ of the body, 16%
More informationMULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question.
Exam Name MULTIPLE CHOICE. Choose the one alternative that best completes the statement or answers the question. 1) All of the following are synthesized along various sites of the endoplasmic reticulum
More informationMicroscopic Anatomy of Inferior Medullary Velum Of Cerebellum
32 J Anat. Soc. India 51(1) 32-34 (2002) Microscopic Anatomy of Of Cerebellum Arora, N.K. Department of Anatomy, Government Medical College, Chandigarh INDIA. Abstract. A study of the inferior medullary
More informationPrepared By Student. Dania Abed Al-majeed. Rahma Raad Hanna. Balqees Mohammed Aasim. Dania Hisham. Rasha Rafiee
Prepared By Student Rahma Raad Hanna Balqees Mohammed Aasim Dania Hisham Dania Abed Al-majeed Rasha Rafiee Epithelia Epithelia can be derived from ectoderm, mesoderm or endoderm -ectoderm gives rise to
More informationHistological and Ultrastructural studies of Caecal tonsil in Chicken (Gallus domesticus)
ISSN: 2319-7706 Volume 4 Number 6 (2015) pp. 63-68 http://www.ijcmas.com Original Research Article Histological and Ultrastructural studies of Caecal tonsil in Chicken (Gallus domesticus) T.A.Kannan 1*,
More informationINTEGUMENTARY 1-Epidermis, 2-Dermis, Structure of thick and thin skin I- Epidermis . Stratum basale
INTEGUMENTARY The skin (integument, cutis ) and its derivatives constitute the integumentary system. It form the external covering of the body and is the largest organ of the body. The skin consists of
More informationEpithelial tumors. Dr. F.F. Khuzin, PhD Dr. M.O. Mavlikeev
Epithelial tumors Dr. F.F. Khuzin, PhD Dr. M.O. Mavlikeev Epithelial tumors Tumors from the epithelium are the most frequent among tumors. There are 2 group features of these tumors: The presence in most
More informationMITOSIS IN DEVELOPING CARDIAC MUSCLE. FRANCIS J. MANASEK. From the Department of Anatomy, Harvard Medical School, Boston, Massachusetts 02115
Published Online: 1 April, 1968 Supp Info: http://doi.org/10.1083/jcb.37.1.191 Downloaded from jcb.rupress.org on June 30, 2018 MITOSIS IN DEVELOPING CARDIAC MUSCLE FRANCIS J. MANASEK. From the Department
More informationANATOMICAL PATHOLOGY TARIFF
ANATOMICAL PATHOLOGY TARIFF A GUIDE TO UTILISATION. The following guidelines have been agreed by consensus of Anatomical Pathologists who are members of the Anatomical Pathologist s Group, or the National
More informationDr. Heba Kalbouneh. Dr. Heba Kalbouneh. Dr. Heba Kalbouneh
Dr. Heba Kalbouneh Dr. Heba Kalbouneh Dr. Heba Kalbouneh Tissue: is a group of cells that serve the same function, they are surrounded by extra cellular matrix. The 4 basic types of tissue: 1. epithelial
More informationAMELOGENESIS. Prof. Shaleen Chandra
AMELOGENESIS Epithelial Enamel Organ Outer Enamel Epithelium Stellate Reticulum Stratum Intermedium Inner Enamel Epithelium Cervical Loop Life Cycle of Ameloblasts Morphogenic stage Organizing Stage Formative
More informationLecture Overview. Chapter 4 Epithelial Tissues Lecture 9. Introduction to Tissues. Epithelial Tissues. Glandular Epithelium
Visual Anatomy & Physiology First Edition Martini & Ober Chapter 4 Lecture 9 Lecture Overview Introduction to Tissues Location General characteristics Functions Classification Glandular Epithelium 2 Where
More informationUltrastructural Study of Human Natural Killer CNK) Cell*)
Hiroshima Journal of Medical Sciences Vol. 31, No. 1, March, 1982 HJIM 31-6 31 Ultrastructural Study of Human Natural Killer CNK) Cell*) Yoshinori KAWAGUCHI, Eishi KITTAKA, Yoshito TANAKA, Takeo TANAKA
More informationNucleolar organiser regions in adenocarcinoma in situ of
J Clin Pathol 1989;42:1276-1280 Nucleolar organiser regions in adenocarcinoma in situ of the endocervix J E CULLIMORE, T P ROLLASON,* T MARSHALLt From the Birmingham and Midland Hospitalfor Women, and
More informationHPV: cytology and molecular testing
HPV: cytology and molecular testing Human Papillomavirus and how we test for it at Medlab Central Palmerston North for Cervical Cancer prevention and management. Developed by Reem Mustafa Cytology and
More informationTHE ULTRASTRUCTURE OF PIGMENTED MELANOMA CELLS IN CONTINUOUS CULTURE*
THE ULTRASTRUCTURE OF PIGMENTED MELANOMA CELLS IN CONTINUOUS CULTURE* FTJNAN HU, M.D.f AND ROBERT R. CARDELL, JR., Pu.D.t The ultrastructure of mammalian melanocytes (1 7) and melanoma cells (8 17) has
More informationAntigen Recognition in the Female Reproductive Tract. II. Endocytosis of Horseradish Peroxidase by Langerhans Cells in Murine Vaginal Epithelium1
BIOLOGY OF REPRODUCTION 45, 261-265 (1991) Antigen Recognition in the Female Reproductive Tract. II. Endocytosis of Horseradish Peroxidase by Langerhans Cells in Murine Vaginal Epithelium1 MARGARET B.
More information