Metabion GmbH, Germany
|
|
|
- Nelson Sherman
- 5 years ago
- Views:
Transcription
1 Metabion GmbH, Germany CUSTOM OLIGO NUCLEOTIDE SYNTHESIS DNA Single Tube Format (price per base) Scale Standard Yield OPC Purified Yield HPLC Purified Yield (µmol) Rs (OD 260 ) Rs (OD 260 ) Rs (OD 260 ) The 0.02 µmol scale can only be ordered for oligos <33 bases (also valid for modified oligos)!!! Plz. Enq. n.a MALDI-T of Analysis = Rs (per analysis) Large Scale Synthesis DNA bases in length Unmodified Final Yield Price for Standard QC package (Rs.Price) 50 mg mg mg mg gram Important Information : 1. Prices are valid for unmodified and single modified oligos only. 2. For Dual Labelled Probes see page 84 For other oligos, please inquire. 3. Price of modification is added to the oligo price. Standard base price applies to all modified oligos exeptions are marked with ** 4. Expected minimum yields are based on a 20mer. Actual yields may vary depending on oligo length and sequence composition. 5. The price per base doubles for oligos longer than 80 bases. 6. No extra charge for internal wobbles. 7.,02 µmol scale only available for oligos up to 33 bases (also applies to modified oligos and Dual Labelled probes). 8. OPC purification only available for oligos up to 40 bases. 9. Rs. 2100/- setup fee for 3 wobbles. Modifications available for oligos delivered in single tube format. 5' Fluorescent Lables 0.02 (µmol) 0.04 (µmol) 0.2 (µmol) 1.0 (µmol) 0.02 (µmol) 0.04 (µmol) 0.2 (µmol) 1.0 (µmol) 5' Modification 3 OD260 5 OD OD OD260 1 OD260 2 OD260 5 OD OD260 6-FAM, Tet Hex, Tamra Plz.Enq. Plz.Enq. Plz.Enq. Plz.Enq Rox, Joe Plz.Enq. Plz.Enq. Plz.Enq. Plz.Enq Fluorescein Cy3, Cy5 Plz.Enq. Plz.Enq. Plz.Enq. Plz.Enq IRD700, IRD800 Plz.Enq. Plz.Enq. Plz.Enq. Plz.Enq Rhodamine Green Plz.Enq. Plz.Enq. Plz.Enq. Plz.Enq Texas Red Yakima Yellow AA1
2 MODIFICATIONS 5' Non-Fluorescent Lables 5' Non-Fluorescent Lables 0.02 (µmol) 0.04 (µmol) 0.2 (µmol) 1.0 (µmol) 0.02 (µmol) 0.04 (µmol) 0.2 (µmol) 1.0 (µmol) 5' Modification 3 OD260 5 OD OD OD260 1 OD260 2 OD260 5 OD OD260 Phosphate C6 Amino C12 Amino Biotin Thiol* Digoxigenin Inosine** Deoxyuridine** Methylcytosin** Radioactive 32 P*** Radioactive 33 P*** * Requires a setup fee of Rs. 15,400/- per order. * * HPLC base price applies for oligos with these modification, if ordered with HPLC. *** 10 pmol radioactive oligo (0.5 MBq) will be delivered. 5 C-5 propynyl-du and C-5 propynyl-dc Modifications (pdu, pdc) Synthesis scale 0,02 µmol 0,04 µmol 0,2 µmol 1,0 µmol 0,02 µmol 0,04 µmol 0,2 µmol 1,0 µmol Minimum Yield* 3 OD260 5 OD OD OD260 3 OD260 5 OD OD OD260 (Rs.Price) (Rs.Price) (Rs.Price) (Rs.Price) (Rs.Price) (Rs.Price) (Rs.Price) (Rs.Price) Price * based on a 20mer, for oligos>33 bases in length, yield guarantee does not apply. ** Please note, that HPLC prices for internal modifications will be added to the fixed prices of real-time PCR probes according to the respective scale Note: C5-propynyl-dU (pdu) raising melting temperature by 1.7 C per substitution; C5-propynyl-dC (pdc) raising melting temperature by 2.8 C per substitution. MODIFICATIONS 3' Non-Fluorescent Lables 3 Non-Fluorescent Labels - Haptens / Base Analoga / Linkers 0.02 (µmol) 0.04 (µmol) 0.2 (µmol) 1.0 (µmol) 0.02 (µmol) 0.04 (µmol) 0.2 (µmol) 1.0 (µmol) 3' Modification 3 OD260 5 OD OD OD260 1 OD260 2 OD260 5 OD OD260 Phosphate C7 Amino Biotin Thiol Methylcytosin** ddc Inosine** Deoxyuridine** Dabcyl Plz.Enq. Plz.Enq. Plz.Enq. Plz.Enq BHQ-1 Plz.Enq. Plz.Enq. Plz.Enq. Plz.Enq BHQ-2 Plz.Enq. Plz.Enq. Plz.Enq. Plz.Enq BHQ-3 Plz.Enq. Plz.Enq. Plz.Enq. Plz.Enq Note: ** HPLC base price applies for oligos with these modifications, if ordered with HPLC. AA2
3 3' Fluorescent Lables 0.02 (µmol) 0.04 (µmol) 0.2 (µmol) 1.0 (µmol) 3' Modification 3 OD 260* 5 OD 260* 12 OD 260* 50 OD 260* Rs Rs Rs Rs 6-FAM Hex Tamra Fluorescein Cy3, Cy Prices for 3'-Fluorescent modifications are valid up to 40 bases. All prices for 3'-Fluorescent modifications include HPLC purification! Internal Fluorescent Lables Internal 0.02 (µmol) 0.04 (µmol) 0.2 (µmol) 1.0 (µmol) Modification 3 OD OD OD OD 260 Rs Rs Rs Rs Fluorescein FITC Tamra Cy Prices for Internal Fluorescent modifications are valid upto 40 bases. - Listed Internal Fluorescent modifications require a T to be attached. - All prices for Internal Fluorescent modifications include HPLC purification! Internal Non-Fluorescent Labels - Haptens / Base Analoga / Linkers Internal 0.02 (µmol) 0.04 (µmol) 0.2 (µmol) 1.0 (µmol) 0.02 (µmol) 0.04 (µmol) 0.2 (µmol) 1.0 (µmol) Modification 3 OD 260* 5 OD 260* 12 OD 260* 50 OD 260* 1 OD 260* 2 OD 260* 5 OD 260* 12 OD 260* C2 Amino dt Plz.Enq. Plz.Enq. Plz.Enq. Plz.Enq Biotin dt* Plz.Enq. Plz.Enq. Plz.Enq. Plz.Enq Methylcytosin** Inosin** Deoxyuridine * Requires a Rs. 35,000/- septup fee per order. ** HPLC base price applies for oligos with these modification, if ordered with HPLC Internal C-5 propynyl-du and C-5 propynyl-dc Modifications (pdu, pdc) Synthesis scale 0,02 µmol 0,04 µmol 0,2 µmol 1,0 µmol 0,02 µmol 0,04 µmol 0,2 µmol 1,0 µmol Minimum Yield* 3 OD260 5 OD OD OD260 3 OD260 5 OD OD OD260 (Rs.Price) (Rs.Price) (Rs.Price) (Rs.Price) (Rs.Price) (Rs.Price) (Rs.Price) (Rs.Price) Price * based on a 20mer, for oligos>33 bases in length, yield guarantee does not apply. ** Please note, that HPLC prices for internal modifications will be added to the fixed prices of real-time PCR probes according to the respective scale Note: C5-propynyl-dU (pdu) raising melting temperature by 1.7 C per substitution; C5-propynyl-dC (pdc) raising melting temperature by 2.8 C per substitution. Antisense oligos S-Oligos Available for Oligos delivered in single tube format. - Full PTOs come with HPLC and Cartridge purification! - For other antisense oligos, such as 2' O-Methyl RNA, please inquire. - For part PTOs, we recommend the cartridge purification. Phosphorothioates (PTOs) Scale Yield Price (µmol) (OD 260 ) Per Base Rs Larger scales Please inquire! Cartridge Purification AA3
4 DNA M MTP Format High-Throughput Oligos - Order and Delivery format 96 / 384 MTP - Orders with more than 48 oligos qualify for MTP format % MALDI ToF check on all oligos! - Electronic transmission of synthesis report. - Barcoded plates available on request. LNA MTP Modification Availability Modification 5' 3' 6-FAM yes no Biotin yes yes C6 Amino yes no C7 Amino no yes C12 Amino yes no Fluorescein yes no HEX yes no Phospahte yes no TET yes no Available for Oligos delivered in single tube format. Scale Price per Base LNA Price per Base DNA Rs. Rs µmol µmol µmol Larger Scales please inquire please inquire HPLC is included. 3' LNA Modifications Modification 0,04 µmol 0,2 µmol 1,0 µmol 10 nmol 25 nmol 80 nmol (Rs.Price) (Rs.Price) (Rs.Price) Amino Phosphate Rox5/ Cy NHS Fam-5/ Tamra-5/ BHQ DNA MTP format (price per base) Project Volume 0.02 (µmol) 0.04 (µmol) 0.2 (µmol) (number of oligos) [<33nt] (upto 70mers) (upto 60mers) 3 OD OD OD 260 Rs Rs Rs < Plz Enq. Plz Enq. Plz Enq Plz Enq. Plz Enq. Plz Enq. > Plz Enq. Plz Enq. Plz Enq. This is only a rough price per base indication! Actual prices should always be neogitated individually, as they depend on the respective requirements concerning logistics (concerntration adjustment, lyophilised or liquid shipping status, order and delivery rotations )! * Guaranteed yields apply for a 20 mer +/-20%! For oligos > 33 bases, we cannot guarantee yield. 5' LNA Modifications Modification 0,04 µmol 0,2 µmol 1,0 µmol 10 nmol 25 nmol 80 nmol (Rs.Price) (Rs.Price) (Rs.Price) Amino-C Amino-C Phosphate Rox5/ Cy NHS FAM Fam-5/ HEX JOE Tamra-5/ TET Biotin Digoxigenin Internal LNA Modifications Modification 0,04 µmol 0,2 µmol 1,0 µmol 10 nmol 25nmol 80 nmol (Rs.Price) (Rs.Price) (Rs.Price) Inosine Deoxyuridine LC Cyan500 3 Phos 3 LC Fluo 3 unmodified internal LC Fluo (Rs.Price) (Rs.Price) (Rs.Price) (Rs.Price) 5 LC Cyan LC Cyan LC Cyan LC Cyan LC Cyan LC Cyan LC Cyan LC Cyan500 Plz.Enq. Plz.Enq. Plz.Enq. Plz.Enq. AA4
5 Dual Labelled Fluorogenic Probes Prices include HPLC purification and Maldi-ToF quality check to ensure probes are of highest quality! Price per probe. No base charges apply! Valid up to 40 bases. For longer oligos, other reporter/quencher combinations or larger volume orders, please contact us. Dual labeled fluorogenic probes pricing Scale Price Band Price Band Price Band Yield range (><)* Pricel in ** Pricel in ** Pricel in ** >/= 5 < >/=10 < >/=20 < >/=30 < >/=50 < >/=70 Plz. Inq. Plz. Inq. Plz. Inq. * based on oligonucleotides of bases in length; ** incuding HPLC purification and QC by Mass-Check 5'Reporter/3'Quencher combinations 3 Quencher Abs range TAMRA BHQ-1 BHQ-2 BHQ-3 Eclipse Dabcyl (nm) Abs max (nm) ' Reporter Price Band Price Band Price Band Price Band Price Band Price Band Abs (nm)* Em (nm)* LC CYAN Fluo FAM Rhodamine Green TET JOE Yakima Yellow Hex Cy TAMRA ROX Texas Red LC Red LC Red Cy Cy IRD * Please note that absorption and emission wavelengths are influenced by the solvent (ph, salt conditions, etc.), oligo sequence and other factors. AA5
6 DNA Real-time PCR Probes - LightCycler Probes - Prices include HPLC purification and MALDI ToF quality check to ensure probes are of the highest quality! - No base charges apply for oligos up to 33 bases. For each base after 33, a 560 Rs. base charge will apply. - LightCycler probes are delivered in defined molar amounts of 1 to 30 nmol, at a length from 15 to 40 nt. - For probe design, larger amounts, large volume orders or other special considerations, please contact us. Product Delivered No. of Price Quantity Tests** Rs in nmol) 5 LC610/640/670-3 Phosphate LC705-3 Phosphate LC610/ Internal LC610/ Fluorescein RNA Custom RNA Oligo All RNA Oligos are HPLC purified! Unmodified or modified oligos, such as sirna, Aptamers and Ribozymes/Chimeras. From 2 to 80 bases, used in a variety complex applications. Annealing buffer and protocol available on request. Internal RNA Modifications Modification 0,2 µmol 1,0 µmol 35 nmol* 70 nmol* (Rs.Price) (Rs.Price) DNA (Price per Base) Inosine Deoxyuridine PTO please inquire please inquire *Expected minimum yield based on a 20mer. ** Requires a Rs.5600setup fee per order. Scale Price per Base Price per Oligo > 12 bases <12 bases Yield nmol (Rs.Price) (Rs.Price) 0,2 µmol ,0 µmol Larger Scale Synthesis RNA DsiRNA bases in Dicer sirna length Unmodified 27 mer Final Yield Price for Standard Price for Standard QC package QC package (Rs.Price) (Rs.Price) 50 mg mg mg mg gram AA6
7 5 RNA Modifications 3 RNA Modifications 0,2 µmol 1,0 µmol 0,2 µmol 1,0 µmol Modification 35 nmol* 70 nmol* Modification 35 nmol* 70 nmol* (Rs.Price) (Rs.Price) (Rs.Price) (Rs.Price) Fam Fam Hex Tamra Tet Fluorescein Tamra Amino Cy3 / Cy Biotin Amino Cholesteryl Biotin Spacer C Cholesteryl Thiol Phosphate PTO Thiol DNA (Price per Base) DNA (Price per Base) Inosine Deoxyuridine PTO Plz.Enq. Plz.Enq AA7
Superior Fluorescent Labeling Dyes Spanning the Full Visible Spectrum...1. Trademarks: HiLyte Fluor (AnaSpec, Inc.)
Table of Contents Fluor TM Labeling Dyes Superior Fluorescent Labeling Dyes Spanning the Full Visible Spectrum....1 Fluor TM 405 Dye, an Excellent Alternative to Alexa Fluor 405 & DyLight 405....2 Fluor
Solid-Phase Purification of Synthetic DNA Sequences. Serge L. Beaucage, Ph.D. Chief, Laboratory of Biological Chemistry FDA-CDER
Solid-Phase Purification of Synthetic DNA Sequences Serge L. Beaucage, Ph.D. Chief, Laboratory of Biological Chemistry FDA-CDER The one who follows the crowd will usually go no further than the crowd.
Instructions for Use. RealStar Influenza S&T RT-PCR Kit /2017 EN
Instructions for Use RealStar Influenza S&T RT-PCR Kit 3.0 01/2017 EN RealStar Influenza S&T RT-PCR Kit 3.0 For research use only! (RUO) 163003 INS-163000-EN-S02 96 01 2017 altona Diagnostics GmbH Mörkenstr.
For in vitro Veterinary Diagnostics only. Kylt Rotavirus A. Real-Time RT-PCR Detection.
For in vitro Veterinary Diagnostics only. Kylt Rotavirus A Real-Time RT-PCR Detection www.kylt.eu DIRECTION FOR USE Kylt Rotavirus A Real-Time RT-PCR Detection A. General Kylt Rotavirus A products are
Introduction to the Oligo HTCS Systems. Novatia, LLC
Introduction to the Oligo HTCS Systems Novatia, LLC What is the Oligo HTCS and what can it do? Automated ESI/MS system that can analyze up to 14 oligonucleotide samples/day (>3/day with twin autosampler)
Instructions for Use. RealStar Influenza Screen & Type RT-PCR Kit /2017 EN
Instructions for Use RealStar Influenza Screen & Type RT-PCR Kit 4.0 05/2017 EN RealStar Influenza Screen & Type RT-PCR Kit 4.0 For research use only! (RUO) 164003 INS-164000-EN-S01 96 05 2017 altona
Triptycene-Based Small Molecules Modulate (CAG) (CTG) Repeat Junctions
Electronic Supplementary Material (ESI) for Chemical Science. This journal is The Royal Society of Chemistry 2015 Triptycene-Based Small Molecules Modulate (CAG) (CTG) Repeat Junctions Stephanie A. Barros
Chlamydia pneumoniae PCR reagents Detection with real time PCR reagents
Chlamydia pneumoniae PCR reagents Detection with real time PCR reagents Overview:... 1 Products... 2 C. pneumoniae FAM-BHQ1 Primer-probe PP3400 0.055ml... 2 AttoMaster 2X Mix for qpcr AM10 1.25 ml... 2
High-Throughput Analysis of Oligonucleotides using Automated Electrospray Ionization Mass Spectrometry
High-Throughput Analysis of Oligonucleotides using Automated Electrospray Ionization Mass Spectrometry Mark E. Hail 1, Brian Elliott 2, Kerry Nugent 3, Jeffrey L. Whitney 1, and David J. Detlefsen 1 1
Systemic RNAi Therapuetics for the Treatment of HIV-1 Prof. John J. Rossi
Systemic RNA Interference (RNAi) Therapeutics for the of HIV-1 John J. Rossi, Professor and chairman Department of Molecular and Cellular Biology, Beckman Research Institute of the City of Hope Duarte
Aeris. Precision Engineered Core- Shell Particles for Ultra-High Resolution BioSeparations. Aeris PEPTIDE. Aeris WIDEPORE
Aeris Precision Engineered Core- Shell Particles for Ultra-High Resolution BioSeparations Aeris is a specialized line of reversed phase core-shell UHPLC columns, built exclusively for the ultra-high performance
Supporting Information
Translation of DNA into Synthetic N-Acyloxazolidines Xiaoyu Li, Zev. J. Gartner, Brian N. Tse and David R. Liu* Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts
RealLine Mycoplasma genitalium Str-Format
Instructions for use ASSAY KIT FOR THE QUALITATIVE DETECTION OF MYCOPLASMA GENITALIUM DNA BY REAL-TIME PCR METHOD In vitro Diagnostics () VBD4396 96 Tests valid from December 2018 Rev06_1218_EN Page 1
Spherical Nucleic Acids For Advanced Wound Healing Applications Chad A. Mirkin
Spherical Nucleic Acids For Advanced Wound Healing Applications Chad A. Mirkin Departments of Chemistry, Infectious Disease, Materials Science & Engineering, Chemical & Biological Engineering, and Biomedical
Supporting Information
Supporting Information Cyclic Peptidyl Inhibitors against Human Peptidyl-Prolyl Isomerase Pin1 Tao Liu, Yu Liu, Hung-Ying Kao, *,, and Dehua Pei Table of Contents: Table S1. Structures of amino acid building
Analysis report ORAC Europe BV
Analysis report ORAC Europe BV initials investigator Customer name: Hak Agrofeed BV att. Mr. B. Hak Leemansstraat 2 4251 LD Werkendam The Netherlands Amount of samples delivered: 63 samples Date of sample
Influenza A viruses Detection with real time RT-PCR reagents
Influenza A viruses Detection with real time RT-PCR reagents Overview:... 1 Products... 2 Influenza A matix FAM-BHQ1 PP500 0.055ml... 2 Influenza A Plasmid 200 pg/ml PLAS500 0.25ml... 2 Detection Influenza
Hepatitis B Virus Genemer
Product Manual Hepatitis B Virus Genemer Primer Pair for amplification of HBV Viral Specific Fragment Catalog No.: 60-2007-10 Store at 20 o C For research use only. Not for use in diagnostic procedures
Overview of the Expressway Cell-Free Expression Systems. Expressway Mini Cell-Free Expression System
Overview of the Expressway Cell-Free Expression Systems The Expressway Cell-Free Expression Systems use an efficient coupled transcription and translation reaction to produce up to milligram quantities
PRIMETECH. Контактная информация:
PRIMETEC REAGETS FR MLECULAR BILGY DA/RA SYTESIS AD AALYSIS Контактная информация: 2018 ABUT: Primetech ALC was founded in 2007 as a spin-off company in the Institute of Physical rganic Chemistry (Laboratory
Supporting Information. Controlled Nucleation and Growth of DNA Tile Arrays within Prescribed DNA Origami Frames and Their Dynamics
Supporting Information Controlled Nucleation and Growth of DNA Tile Arrays within Prescribed DNA Origami Frames and Their Dynamics Wei Li, Yang Yang, Shuoxing Jiang, Hao Yan, Yan Liu Department of Chemistry
Quantity in. Unit of Measure. (If Applicable) 1 each Anode Buffer Reservoir Jar 1 Applied Biosystems $101.65
CONTRACT 1-09-65-05 Consumable DNA Kits and Reagent Supplies Supplement 2 2/24/2011 ATTACHMENT A Contract Pricing (Rev 2/24/11) Contract Line Item 1 each Anode Buffer Reservoir Jar 1 Applied Biosystems
Components of a Mass Spectrometer
Components of a Mass Spectrometer Sample Introduction Inlet GC LC Direct Insertion (Syringe/Probe) Ionization Ion Separation Ion Detection Ion Source EI,CI,,, MALDI Mass Analyzer Under vacuum TOF, Quadrupole,
ab Homocysteine Assay Kit (Fluorometric) 1
Version 1 Last updated 25 May 2018 ab208559 Homocysteine Assay Kit (Fluorometric) For the measurement of homocysteine in mammalian plasma and serum. This product is for research use only and is not intended
Laser Induced Fluorescence (LIF) Technique Part - 4
ere 545X class notes #39# Laser nduced Fluorescence LF echnique Part - 4 ui u Department o erospace Engineering owa State Universit mes owa 5 U.S. opright b Dr. ui u @ owa State Universit. ll Rights Reserved!
Human Rotavirus A. genesig Advanced Kit. Non structural protein 5 (NSP5) 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Human Rotavirus A Non structural protein 5 (NSP5) genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to Human Rotavirus A Rotavirus is a genus
OVERVIEW OF CURRENT IDENTIFICATION SYSTEMS AND DATABASES
OVERVIEW OF CURRENT IDENTIFICATION SYSTEMS AND DATABASES EVERY STEP OF THE WAY 1 EVERY STEP OF THE WAY MICROBIAL IDENTIFICATION METHODS DNA RNA Genotypic Sequencing of ribosomal RNA regions of bacteria
Supporting information to Amino-functional polyester dendrimers based on bis-mpa as nonviral vectors for sirna delivery
Supporting information to Amino-functional polyester dendrimers based on bis-mpa as nonviral vectors for sirna delivery P. Stenström, D. Manzanares, Y. Zhang, V. Ceña and M. Malkoch* * To whom correspondence
EpiQuik Circulating Acetyl Histone H3K18 ELISA Kit (Colorimetric)
EpiQuik Circulating Acetyl Histone H3K18 ELISA Kit (Colorimetric) Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE Uses: The EpiQuik Circulating Acetyl Histone H3K18 ELISA Kit (Colorimetric)
Instructions. Fuse-It-mRNA easy. Shipping and Storage. Overview. Kit Contents. Specifications. Note: Important Guidelines
Membrane fusion is a highly efficient method for transfecting various molecules and particles into mammalian cells, even into sensitive and primary cells. The Fuse-It reagents are cargo-specific liposomal
gastroplexvirus real time RT-PCR Kit
Instruction for Use gastroplexvirus real time RT-PCR Kit For the qualitative in-vitro detection of RNA from Rotavirus, Norovirus (GI and GII), and DNA from Adenovirus in clinical specimens, environmental
RealLine HIV quantitative Str-Format
Instructions for use DETECTION AND QUANTIFICATION OF THE HUMAN IMMUNODEFICIENCY VIRUS RNA BY REAL TIME PCR Research Use Only (RUO) Attention! Please read the information about quantification process carefully!
Translating Molecular Detection into a Temperature Test Using. Target-Responsive Smart Thermometer
Electronic Supplementary Material (ESI) for Chemical Science. This journal is The Royal Society of Chemistry 2018 Supporting Information Translating Molecular Detection into a Temperature Test Using Target-Responsive
Supporting Information
Supporting Information Dauvillée et al. 10.1073/pnas.0907424106 Fig. S1. Iodine screening of the C. cohnii mutant bank. Each single colony was grown on rich-medium agar plates then vaporized with iodine.
Table S1. Oligonucleotides used for the in-house RT-PCR assays targeting the M, H7 or N9. Assay (s) Target Name Sequence (5 3 ) Comments
SUPPLEMENTAL INFORMATION 2 3 Table S. Oligonucleotides used for the in-house RT-PCR assays targeting the M, H7 or N9 genes. Assay (s) Target Name Sequence (5 3 ) Comments CDC M InfA Forward (NS), CDC M
WHO Prequalification of In Vitro Diagnostics PUBLIC REPORT. Product: Alere q HIV-1/2 Detect WHO reference number: PQDx
WHO Prequalification of In Vitro Diagnostics PUBLIC REPORT Product: Alere q HIV-1/2 Detect WHO reference number: PQDx 0226-032-00 Alere q HIV-1/2 Detect with product codes 270110050, 270110010 and 270300001,
Table showing various modified oligonucleotides synthesized using UnyLinker loaded solid supports.
Table showing various modified oligonucleotides synthesized using UnyLinker loaded solid supports. Oligo Synthesized Sequence 5 3 Oligo 1 2 -O-Methoxyethyl modified phosphorothioate 2- nucleotide gapmer
Avian Influenza A H5N8
TM Primerdesign Ltd Avian Influenza A H5N8 Hemagglutinin (HA) gene & Neuraminidase (NA) gene genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Avian Influenza
Analysis report ORAC Europe BV
Analysis report ORAC Europe BV initials investigator Customer name: Hak Agrofeed BV att. Mr. B. Hak Leemansstraat 2 4251 LD Werkendam The Netherlands Amount of samples delivered: 10 samples Date of sample
Electronic Supporting Information for
Electronic Supplementary Material (ESI) for RSC Advances. This journal is The Royal Society of Chemistry 2015 Electronic Supporting Information for Rhodamine based Turn-On Fluorescent Probe for Pb(II)
Tunable Hydrophobicity in DNA Micelles Anaya, Milena; Kwak, Minseok; Musser, Andrew J.; Muellen, Klaus; Herrmann, Andreas; Müllen, Klaus
University of Groningen Tunable Hydrophobicity in DNA Micelles Anaya, Milena; Kwak, Minseok; Musser, Andrew J.; Muellen, Klaus; Herrmann, Andreas; Müllen, Klaus Published in: Chemistry DOI: 10.1002/chem.201001816
Plasmids Western blot analysis and immunostaining Flow Cytometry Cell surface biotinylation RNA isolation and cdna synthesis
Plasmids psuper-retro-s100a10 shrna1 was constructed by cloning the dsdna oligo 5 -GAT CCC CGT GGG CTT CCA GAG CTT CTT TCA AGA GAA GAA GCT CTG GAA GCC CAC TTT TTA-3 and 5 -AGC TTA AAA AGT GGG CTT CCA GAG
altona RealStar Instructions for Use RealStar CMV PCR Kit /2017 EN DIAGNOSTICS
altona DIAGNOSTICS Instructions for Use RealStar CMV PCR Kit 1.2 08/2017 EN RealStar RealStar CMV PCR Kit 1.2 For research use only! (RUO) 021202 INS-021200-EN-S01 48 08 2017 altona Diagnostics GmbH Mörkenstr.
Rotavirus A. genesig Standard Kit. DNA testing. Everything... Everyone... Everywhere... Non structural protein 5 (NSP5) 150 tests.
TM Primerdesign Ltd TM Primerdesign Ltd Rotavirus A Non structural protein 5 (NSP5) genesig Standard Kit 150 tests DNA testing Everything... Everyone... Everywhere... For general laboratory and research
Supplementary Data 1. Alanine substitutions and position variants of APNCYGNIPL. Applied in
Supplementary Data 1. Alanine substitutions and position variants of APNCYGNIPL. Applied in Supplementary Fig. 2 Substitution Sequence Position variant Sequence original APNCYGNIPL original APNCYGNIPL
Supporting Information
Supporting Information The Effects of Spacer Length and Composition on Aptamer-Mediated Cell-Specific Targeting with Nanoscale PEGylated Liposomal Doxorubicin Hang Xing +, [a] Ji Li +, [a] Weidong Xu,
Product Guide for LudgerSep TM C3 anion exchange HPLC Column for Glycan Analysis
Product Guide for LudgerSep TM C3 anion exchange HPLC Column for Glycan Analysis (Ludger Product Code: LS-C3-7.5x75) Ludger Document # LS-C3-Guide-v3.0 Ludger Ltd Culham Science Centre Oxford OX14 3EB
Service and Collaboration
Summary Section 7 Introduction 68 Exosome and Microvesicle Isolation 69 EV protein quantification, sceening and profiling 69 Nanoparticle Tracking Analysis (NTA) 70 EV Nucleic Acid isolation 70 Nucleic
A Liposome-based Nanostructure for Aptamer Directed Delivery. Supporting Information (SI)
A Liposome-based Nanostructure for Aptamer Directed Delivery Huaizhi Kang, Meghan B. O'Donoghue, Haipeng Liu and Weihong Tan* Center for Research at the Bio/Nano Interface, Department of Chemistry and
RealLine HCV Qualitative Str-Format
Instructions for use REAL TIME PCR DETECTION KIT FOR THE HEPATITIS C VIRUS RNA (HCV) Research Use Only (RUO) (Str-format) VBD0795 96 Tests valid from: October 2018 Rev05_1018_EN Page 1 of 8 Explanation
Supplementary Figure S1. Venn diagram analysis of mrna microarray data and mirna target analysis. (a) Western blot analysis of T lymphoblasts (CLS)
Supplementary Figure S1. Venn diagram analysis of mrna microarray data and mirna target analysis. (a) Western blot analysis of T lymphoblasts (CLS) and their exosomes (EXO) in resting (REST) and activated
Elementary tetrahelical protein design for diverse oxidoreductase functions
Title: Elementary tetrahelical protein design for diverse oxidoreductase functions Authors: Tammer A. Farid 1,4, Goutham Kodali 1,4, Lee A. Solomon 1,4, Bruce R. Lichtenstein 1,2, Molly M. Sheehan 1, Bryan
Human Rotavirus A. genesig Standard Kit. Non structural protein 5 (NSP5) 150 tests. Primerdesign Ltd. For general laboratory and research use only
TM Primerdesign Ltd Human Rotavirus A Non structural protein 5 (NSP5) genesig Standard Kit 150 tests For general laboratory and research use only 1 Introduction to Human Rotavirus A Rotavirus is a genus
MutaPLEX GastroSys 1. real time RT-PCR kit
MutaPLEX GastroSys 1 real time RT-PCR kit For the qualitative in vitro detection of RNA of rotavirus, norovirus (GI and GII), and DNA of adenovirus in clinical specimens, environmental and food samples
of Serum Oxidation V max ) and
SUPPLEMENTAL DATA Paradoxical Effects of Serum Amyloid A on the Lipoprotein Oxidation Suggest a New Antioxidant t Function for SAA Shobini Jayaraman, Christian Haupt, and Olga Gursky Figure S1. Lipid peroxidation
RealLine HBV / HCV / HIV Str-Format
Instructions for use ASSAY KIT FOR THE QUALITATIVE AND DIFFERENTIAL DETECTION OF HEPATITIS B VIRUS DNA, HEPATITIS C VIRUS RNA, AND HUMAN IMMUNODEFICIENCY VIRUS TYPES 1 AND 2 RNA USING THE PCR/RT-PCR METHOD
Fluorogenic Substrates
PENTAPHARM Fluorogenic Substrates Pentapharm Ltd. offers a broad spectrum of fluorogenic peptide substrates for research, inprocess and quality control, employing the fluorogenic group AMC (7-Amino-4-methylcoumarin).
MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells
MicroRNA sponges: competitive inhibitors of small RNAs in mammalian cells Margaret S Ebert, Joel R Neilson & Phillip A Sharp Supplementary figures and text: Supplementary Figure 1. Effect of sponges on
artus CMV TM PCR Kit Handbook
artus CMV TM PCR Kit Handbook 24 (catalog no. 4503163) 96 (catalog no. 4503165) Quantitative in vitro Diagnostics For use with the ABI PRISM 7000, 7700, and 7900HT Sequence Detection Systems December 2014
- 1 - Cell types Monocytes THP-1 cells Macrophages. LPS Treatment time (Hour) IL-6 level (pg/ml)
Supplementary Table ST1: The dynamic effect of LPS on IL-6 production in monocytes and THP-1 cells after GdA treatment. Monocytes, THP-1 cells and macrophages (5x10 5 ) were incubated with 10 μg/ml of
Facile Cu(II) mediated conjugation of thioesters and thioacids to peptides and proteins under mild conditions
Electronic Supplementary Material (ESI) for Organic & Biomolecular Chemistry. This journal is The Royal Society of Chemistry 2018 Facile Cu(II) mediated conjugation of thioesters and thioacids to peptides
Caution: For Laboratory Use. A product for research purposes only. Eu-W1024 ITC Chelate & Europium Standard. Product Number: AD0013
TECHNICAL DATA SHEET Lance Caution: For Laboratory Use. A product for research purposes only. Eu-W1024 ITC Chelate & Europium Standard Product Number: AD0013 INTRODUCTION: Fluorescent isothiocyanato-activated
respirarna 2.0 real time RT-PCR Kit
Instruction for Use respirarna 2.0 real time RT-PCR Kit For the in-vitro detection of the RNA of Influenzavirus A, Influenzavirus B and Respiratory Syncytial Virus in clinical specimens. G01084-32 G01084-96
Phosphate buffered saline (PBS) for washing the cells TE buffer (nuclease-free) ph 7.5 for use with the PrimePCR Reverse Transcription Control Assay
Catalog # Description 172-5080 SingleShot Cell Lysis Kit, 100 x 50 µl reactions 172-5081 SingleShot Cell Lysis Kit, 500 x 50 µl reactions For research purposes only. Introduction The SingleShot Cell Lysis
RealLine HIV qualitative Str-Format
Instructions for Use REAL TIME PCR DETECTION KIT FOR HUMAN IMMUNODEFICIENCY VIRUS RNA Research Use Only (RUO) RealLine HIV Qualitative (Str-format) VBD0196 96 Tests valid from July 2016 Rev01072016_EN
Human Immunodeficiency Virus-1 (HIV-1) Genemer. Primer Pair for amplification of HIV-1 Specific DNA Fragment
Product Manual Human Immunodeficiency Virus-1 (HIV-1) Genemer Primer Pair for amplification of HIV-1 Specific DNA Fragment Catalog No.: 60-2002-10 Store at 20 o C For research use only. Not for use in
CycloLab. a cyclodextrin R&D company. Special offer
CycloLab a cyclodextrin R&D company Special offer valid from 1 st December, 2017 till 31 st December, 2017 Dear Customer, A special 25% discount is granted from the price displayed on the website for all
UV Tracer TM Maleimide NHS ester
UV Tracer TM Maleimide HS ester Product o.: 1020 Product ame: UV-Tracer TM Maleimide-HS ester Chemical Structure: Chemical Composition: C 41 H 67 5 18 Molecular Weight: 1014.08 Appearance: Storage: Yellow
Hahn Lab Dye Kit. Contents of dye kit:
Hahn Lab Dye Kit Contents of dye kit: dye name donor/acceptor code references (see below) Mero53 si-so-ia 4 Mero58 TD-BA-sIA - Mero59 TD-SO-sIA - Mero60 I-Pht-sIA 1 Mero61 I-BA-sIA 1 Mero62 I-TBA-sIA 1
WHO Prequalification of Diagnostics Programme PUBLIC REPORT. Product: VERSANT HIV-1 RNA 1.0 Assay (kpcr) Number: PQDx
WHO Prequalification of Diagnostics Programme PUBLIC REPORT Product: VERSANT HIV-1 RNA 1.0 Assay (kpcr) Number: PQDx 0115-041-00 Abstract The VERSANT HIV-1 RNA 1.0 Assay (kpcr) with product codes 10375763,
ratmdr1b NMQ Ves Tr Assay Protocol CAT. NO. SBVT11
ratmdr1b NMQ Ves Tr CAT. NO. SBVT11 Page 1 of 10 Determination of the interaction of drugs with the rat Mdr1b transporter using the 3H-NMQ vesicular transport assay (for 96 well filterplates) For the following
Oligo Sequence* bp %GC Tm Hair Hm Ht Position Size Ref. HIVrt-F 5 -CTA-gAA-CTT-TRA-ATg-CAT-ggg-TAA-AAg-TA
Human immunodeficiency virus (HIV) detection & quantitation by qrt-pcr (Taqman). Created on: Oct 26, 2010; Last modified by: Jul 17, 2017; Version: 3.0 This protocol describes the qrt-pcr taqman based
Human influenza A virus subtype (H3)
PCRmax Ltd TM qpcr test Human influenza A virus subtype (H3) Haemoglutinin H3 gene 150 tests For general laboratory and research use only 1 Introduction to Human influenza A virus subtype (H3) Influenza,
Carotenoid Oxygenases from Camellia sinensis, Osmanthus fragrans, and Prunus persica nucipersica
Carotenoid Oxygenases from Camellia sinensis, Osmanthus fragrans, and Prunus persica nucipersica - Kinetics and Structure - Von der Fakultatfur Lebenswissenschaften der Technischen Universitat Carolo-Wilhelmina
RNA extraction, RT-PCR and real-time PCR. Total RNA were extracted using
Supplementary Information Materials and Methods RNA extraction, RT-PCR and real-time PCR. Total RNA were extracted using Trizol reagent (Invitrogen,Carlsbad, CA) according to the manufacturer's instructions.
LANCE Eu-W1024 ITC Chelate & Europium Standard AD0013 Development grade
AD0017P-4 (en) 1 LANCE Eu-W1024 ITC Chelate & Europium Standard AD0013 Development grade INTRODUCTION Fluorescent isothiocyanato-activated (ITC-activated) Eu-W1024 chelate is optimized for labelling proteins
Branched Chain Amino Acid (Leu, Ile, Val) Assay Kit
Branched Chain Amino Acid (Leu, Ile, Val) Assay Kit Catalog Number KA0824 100 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 General
Blocking the expression of the hepatitis B virus S gene in hepatocellular carcinoma cell lines with an anti-gene locked nucleic acid in vitro
Blocking the expression of the hepatitis B virus S gene in hepatocellular carcinoma cell lines with an anti-gene locked nucleic acid in vitro Y.-B. Deng, H.-J. Qin, Y.-H. Luo, Z.-R. Liang and J.-J. Zou
Phospholipid Assay Kit
Phospholipid Assay Kit Catalog Number KA1635 100 assays Version: 06 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Intended Use... 3 Background... 3 General Information...
Chemical Surface Transformation 1
Chemical Surface Transformation 1 Chemical reactions at Si H surfaces (inorganic and organic) can generate very thin films (sub nm thickness up to µm): inorganic layer formation by: thermal conversion:
Introduction to Cladosporium_spp
Techne qpcr test Cladosporium_spp 150 tests For general laboratory and research use only 1 Introduction to Cladosporium_spp Cladosporium is a genus of slow growing fungi, colonies are mostly dark green
Supporting Information for:
Supporting Information for: Tunable Heptamethine-Azo Dye Conjugate as an IR Fluorescent Probe for the Selective Detection of Mitochondrial Glutathione over Cysteine and Homocysteine Soo-Yeon Lim, 1 Keum-Hee
LDL Uptake Cell-Based Assay Kit
LDL Uptake Cell-Based Assay Kit Item No. 10011125 www.caymanchem.com Customer Service 800.364.9897 Technical Support 888.526.5351 1180 E. Ellsworth Rd Ann Arbor, MI USA TABLE OF CONTENTS GENERAL INFORMATION
Caution: For Laboratory Use. A product for research purposes only. Eu-W1284 Iodoacetamido Chelate & Europium Standard. Product Number: AD0014
TECHNICAL DATA SHEET Lance Caution: For Laboratory Use. A product for research purposes only. Eu-W1284 Iodoacetamido Chelate & Europium Standard Product Number: AD0014 INTRODUCTION: Iodoacetamido-activated
(A) Cell membrane (B) Ribosome (C) DNA (D) Nucleus (E) Plasmids. A. Incorrect! Both prokaryotic and eukaryotic cells have cell membranes.
High School Biology - Problem Drill 03: The Cell No. 1 of 10 1. Which of the following is NOT found in prokaryotic cells? #01 (A) Cell membrane (B) Ribosome (C) DNA (D) Nucleus (E) Plasmids Both prokaryotic
ab HIV-1 Protease Inhibitor Screening Kit (Fluorometric)
Version 1 Last updated 2 March 2017 ab211106 HIV-1 Protease Inhibitor Screening Kit (Fluorometric) For the rapid, sensitive and accurate screening of potential HIV-1 Protease inhibitors. This product is
Cladosporium_spp genesig Advanced Kit
TM Primerdesign Ltd Cladosporium_spp genesig Advanced Kit 150 tests For general laboratory and research use only 1 Introduction to Cladosporium_spp Cladosporium is a genus of slow growing fungi, colonies
Electronic Supplementary Information (ESI) A unique dansyl-based chromogenic chemosensor for rapid and ultrasensitive hydrazine detection
Electronic Supplementary Material (ESI) for Journal of Materials Chemistry B. This journal is The Royal Society of Chemistry 2014 Electronic Supplementary Information (ESI) A unique dansyl-based chromogenic
MALDI Sepsityper Kit
Instructions for Use MALDI Sepsityper Kit Kit for identification of microorganisms from positive blood cultures using the MALDI Biotyper system CARE products are designed to support our worldwide customers
L-Amino Acid Assay Kit
ab65347 L-Amino Acid Assay Kit Instructions for Use For the rapid, sensitive and accurate measurement of L-Amino Acid levels in various samples. This product is for research use only and is not intended
T H E J O U R N A L O F C E L L B I O L O G Y
T H E J O U R N A L O F C E L L B I O L O G Y Supplemental material Stelter et al., http://www.jcb.org/cgi/content/full/jcb.201105042/dc1 S1 Figure S1. Dyn2 recruitment to edid-labeled FG domain Nups.
Supporting Information
Supporting Information A single design strategy for dual sensitive ph probe with a suitable range to map ph in living cells Kang-Kang Yu, Ji-Ting Hou, Kun Li, * Qian Yao, Jin Yang, Ming-Yu Wu, Yong-Mei
Supporting Information. Ligand-mediated Coating of Liposomes with Human Serum Albumin
Supporting Information Ligand-mediated Coating of Liposomes with Human Serum Albumin Hikari Sato, 1 Elnaz Nakhaei, 1 Takahito Kawano, 2,3 Masaharu Murata, 2,3 Akihiro Kishimura, 1,4,5,6 Takeshi Mori, 1,4,5,
Supporting Information. A Two-In-One Fluorescent Sensor With Dual Channels to. Discriminate Zn 2+ and Cd 2+
Electronic Supplementary Material (ESI) for RS Advances Supporting Information A Two-In-One Fluorescent Sensor With Dual hannels to Discriminate Zn 2 and d 2 Li-Kun Zhang, a Guang-Fu Wu, a Ying Zhang,
Nature Protocols: doi: /nprot Supplementary Figure 1. Fluorescent titration of probe CPDSA.
Supplementary Figure 1 Fluorescent titration of probe CPDSA. Fluorescent titration of probe CPDSA (10 um) upon addition of GSH in HEPES (10 mm, ph = 7.4) containing 10% DMSO. Each spectrum was recorded
SC-L-H shared(37) Specific (1)
A. Brain (total 2) Tissue-specific (2) Brain-heart shared (8) Specific (2) CNS-heart shared(68) Specific () Heart (total 4) Tissue-specific () B-L-H shared (37) specific () B. Brain (total 2) Tissue-specific
Cytosolic targeting of macromolecules using a ph-dependent fusogenic peptide in. combination with cationic liposomes
Cytosolic targeting of macromolecules using a ph-dependent fusogenic peptide in combination with cationic liposomes Sachiko Kobayashi, Ikuhiko Nakase, Noriko Kawabata, Hao-hsin Yu, Silvia Pujals, Miki
Human Immunodeficiency Virus Types 1 & 2 and Hepatitis Viruses B and C. genesig PLEX kit. 100 tests. Primerdesign Ltd
Primerdesign Ltd Human Immunodeficiency Virus Types 1 & 2 and Hepatitis Viruses B and C genesig PLEX kit 100 tests For general laboratory and research use only 1 Introduction HIV1 & HIV2 Human immunodeficiency
Malachite Green Phosphate Assay Kit. Item No
Malachite Green Phosphate Kit Item No. 10009325 TABLE OF CONTENTS GENERAL INFORMATION 3 Materials Supplied 4 Precautions 4 If You Have Problems 4 Storage and Stability 4 Materials Needed but Not Supplied
Figure 1. Base your answer to the question on the information and diagram below and on your knowledge of biology.
Name: Teacher: Lathrop Topic 3 cells CR HW #3 Figure 1 Base your answer to the question on the information and diagram below and on your knowledge of biology. Using a microscope and a wet-mount slide,