Effects of cooling on skeletal muscle recovery after exercise. Arthur J. Cheng, Ph.D.

Transcription

1 Effects of cooling on skeletal muscle recovery after exercise Arthur J. Cheng, Ph.D. Researcher Department of Physiology and Pharmacology Cellular Muscle Function Laboratory

2 Recovery from Fatigue - Soccer Arthur J. Cheng 20/02/2018 2

3 Recovery Cold Water Immersion (CWI) Arthur J. Cheng 20/02/2018 3

4 Whole Muscle to Single Fibre Skeletal muscle fibre (cell) Arthur J. Cheng 20/02/2018 4

5 Segment of a Single Fibre Arthur J. Cheng 20/02/2018 5

6 Excitation-Contraction Coupling sarcolemma myoplasm DHPR RyR1 Ca 2+ Ca 2+ Ca 2+ Ca 2+ SERCA Ca 2+ myofilaments

7 Excitation-Contraction Coupling sarcolemma myoplasm DHPR RyR1 SERCA Ca2+ Ca2+ Ca 2+ Ca 2+ Ca 2+ myofilaments

8 Intact single muscle fibre force transducer Micropipette Fluorescent Ca 2+ indicator 120 x tendon single fibre (1 mm) tendon Cheng and Westerblad, 2017 Nat Protoc Arthur J. Cheng 20/02/2018 8

9 Force-[Ca 2+ ] i Relationship 70 Hz 100 Hz 120 Hz Force 50 Hz 30 Hz 10 Hz [Ca 2+ ] i Arthur J. Cheng 20/02/2018 9

10 Fatigue in human muscle fibres (Unpublished data) Arthur J. Cheng 20/02/

11 Glycogen Depletion During Fatigue Before fatigue After fatigue Neilsen, Cheng et al., 2014 J Physiol Neilsen, Cheng et al., 2014 J Physiol Arthur J. Cheng 20/02/

12 SR Ca 2+ Release and Glycogen Nielsen et al., 2009 J Physiol Arthur J. Cheng 20/02/

13 Glycogen Resynthesis is Slow After competitive soccer match Krustrup et al., 2011 Eur J Appl Physiol Arthur J. Cheng 20/02/

14 Recovery Cold Water Immersion Michael Phelps and Ryan Lochte, Rio Olympics 2016 Arthur J. Cheng 20/02/

15 Recovery Cold Water Immersion Post-exercise recovery of contractile function and endurance in humans and mice is accelerated by heating and slowed by cooling skeletal muscle. Cheng, A.J., Willis, S.J., Zinner, C., Chaillou, T., Ivarsson, N., Ortenblad, N., Lanner, J.T., Holmberg, H.C., Westerblad, H. (2017) The Journal of Physiology 595(24): Arthur J. Cheng 20/02/

16 Effects of Temperature on Recovery From Fatigue Cheng et al., 2017 J Physiol Arthur J. Cheng 20/02/

17 Swedish Winter Sports Research Institute Cheng et al., 2017 J Physiol Arthur J. Cheng 17

18 Cooling Depresses Power Output Fatigue Test 1 2 h recovery Fatigue Test 2 Cheng et al., 2017 J Physiol Arthur J. Cheng 20/02/

19 Fatigue in single muscle fibres 28 um Cheng and Westerblad, 2017 Nat Protoc Arthur J. Cheng 20/02/

20 Cooling Depresses SR Ca 2+ Release Fatigue Induction 2 h recovery 30 Hz 36 C 31 C 26 C 16 C 31 Cheng et al., 2017 J Physiol Arthur J. Cheng 20/02/

21 Cooling Slows Force Recovery Submaximal force (30 Hz) Muscle Glycogen Cheng et al., 2017 J Physiol Arthur J. Cheng 20/02/

22 Bottomline: CWI and Recovery Post-exercise timeline 0-2 h: Cooling blunts recovery of strength, endurance, and glycogen resynthesis (Cheng et al., 2017 J Physiol) 0-48 h: 10 min CWI does not reduce muscle inflammation after one session of resistance exercise (Peake et al., 2017 J Physiol) 12 weeks strength training: 10 min CWI after every training session blunts increases in muscle mass and strength (Roberts et al., 2015 J Physiol) Arthur J. Cheng 20/02/

23 Bottomline: Arthur J. Cheng 20/02/

24 Acknowledgements Karolinska Institutet Håkan Westerblad Niklas Ivarsson Johanna Lanner Funding Sources Mid Sweden University Hans Christer Holmberg Sarah Willis Christoph Zinner Örebro University Thomas Chaillou University of Southern Denmark Niels Ørtenblad

25 Thank you! Website: Arthur J. Cheng 20/02/

26 CWI and long-term muscle adaptation 12-week lower body strength training: Twice per week, 8-12 RM (leg press, knee extension, knee flexion, lunges, plyometrics) 1. Recovery: 10min 37 W cycling (ACT) 2. Recovery: 10 min at 10 C (CWI) Roberts et al., 2015 J Physiol Arthur J. Cheng 20/02/

27 What about CWI for inflammation? Exercise: 18 sets x 8-12 reps (squats, knee extension, lunges) 1. Recovery: 10 min 37 W cycling (ACT) 2. Recovery: 10 min at 10 C (CWI) Peake et al., 2017 J Physiol Arthur J. Cheng 20/02/

28 What about CWI for inflammation? mrna expression Peake et al., 2017 J Physiol Arthur J. Cheng 20/02/

29 What about CWI for inflammation? mrna expression Peake et al., 2017 J Physiol Arthur J. Cheng 20/02/

30 Cooling Decreases Endurance After 1h recovery at 26 After 1h recovery at 36 Cheng et al., 2017 J Physiol Arthur J. Cheng 20/02/

31 Fatigue and glycogen depletion 60 min submaximal cycling 1 min supramaximal cycling Bergstrom and Hultman,1967 Gollnick PD et al., 1973 Arthur J. Cheng 20/02/

32 Fatigue in Mouse Single Fibre Force Arthur J. Cheng 20/02/

33 Recovery Cold Water Immersion Arthur J. Cheng 20/02/

34 Cooling Slows Glycogen Resynthesis Cheng et al., 2017 J Physiol Arthur J. Cheng 34

35 Bottomline: CWI for Recovery? Arthur J. Cheng 20/02/