Trichoplus. Eng. Milan Hluchý, PhD.
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1 Eng. Milan Hluchý, PhD.
2 iocont Laboratory The consists of a mixture of eggs, larvae, praepupae and pupae of the species, Trichogramma pintoi.
3 iocont Laboratory The mixture of eggs, larvae, praepupae and pupae of T. pintoi is placed in small light polystyrene capsules.
4 iocont Laboratory The capsules protect the eggs against adverse climatic conditions and predators such as ants, etc.
5 iocont Laboratory
6 iocont Laboratory The cotton bollworm (Helicoverpa armigera)
7 iocont Laboratory The cotton bollworm is a serious pest of many kinds of vegetables.
8 Pheromone traps monitoring of the cotton bollworm.
9 iocont Laboratory Dosage: Helicoverpa armigera tomato 2 3 x 15 caps/gen/ha red pepper 2 3 x 15 caps/gen/ha
10 iocont Laboratory Crop: Tomato in greenhouse Locality: Hungary, Velence, 22 Pest: Cotton bollworm (H. armigera) Date of treatment: 19.7; 27.7; 5.8; 1.8; Dosage: 2x + 3x 2 caps/ha Date of evaluation pieces The time of application
11 iocont Laboratory Effect of on the cotton bollworm in tomato in greenhouse Locality: Hungary, Velence, 22 Dosage:2x + 3x 2 caps/ha Date of treatment: 19.7; 27.7; 5.8; 1.8; Dateb of evaluation: 5.8., Efficacy: 1. evaluation 93,8 % Evaluated sample: 4x 5 fruits 2. evaluation 82,2 % 7 Number of worms / 2 tomato x control 1. evaluation 3x control 2. evaluation Source SP
12 iocont Laboratory Crop: Tomato open field Locality: Želiezovce, 23 Dosage: 5 x 15 caps/ha Pest: Cotton bollworm (H. armigera) Date of treatment: 4.7; 9.7; 16.7; 13.8; 21.8 chemical treatment pieces ,4 l/ha (teflubenzuron) 1 l/ha (fenitrothion),5 l/ha (teflubenzuron),3 l/ha (thiacloprid) ,3 l/ha (thiacloprid) The time of application Date of evaluation
13 iocont Laboratory Effect of on the cotton bollworm in tomato open field Locality: Želiezovce, 23 Dosage: 5 x 15 caps/ha Date of treatment: 4.7; 9.7; 16.7; 13.8; 21.8 Date of evaluation: Evaluated sample: 3 x 1 plants 3 25,9 % Infestation % ,4 % chemical tretment
14 pieces. iocont Laboratory Crop: Tomato open field Locality: Vrbová nad Váhom, SK, 23 Pest: Cotton bollworm (H. armigera) Date of treatment: 4.7; 9.7; 16.7; 13.8; 21.8 Dosage: 5 x 15 caps/ha chemical treatment ,4 l/ha (teflubenzuron) ,4 l/ha (teflubenzuron),4 l/ha (teflubenzuron) ,4 l/ha teflubenzuron + chlorpyrifos 2 l/ha,4 l/ha teflubenzuron + chlorpyrifos 2 l/ha The time of application Date of evaluation 8.9.
15 iocont Laboratory Effect of on the cotton bollworm in tomato open field Locality: Vrbová nad Váhom, 23 Dosage: 5 x 15 caps/ha Date of treatment: 4.7; 9.7; 16.7; 13.8; 21.8 Date of evaluation: 8.9 Evaluated sample: 1 plants 1 83,4 % Efficacy % ,9 % chemical treatment
16 iocont Laboratory Crop: Pepper in greenhouse Locality: Velence, Hungary, 22 Pest: Cotton bollworm (H. armigera) Date of treatment: 19.7; 27.7; 5.8; 1.8; 16.8 Dosage: 2x + 3x 2 caps/ha Date of evaluation pieces The time of application
17 iocont Laboratory Effect of on the cotton bollworm in pepper greenhouse Locality: Velence, Hungary, 22 Dosage: 2x + 3x 2 caps/ha Date of treatment: 19.7; 27.7; 5.8; 1.8; 16.8 Date of evaluation: 5.8., Evaluated sample: 4 x 5 fruits Efficacy: 1. evaluation 94 % 2. evaluation 77,4 % Number worms / 2 peppers x control 1. evaluation 3x control 2. evaluation Source SP
18 pieces iocont Laboratory Crop: Pepper ( Marka, Flamingo) open field Locality: Želiezovce, SK, 23 Pest: Cotton bollworm (H. armigera) Date of treatment: 4.7; 9.7; 16.7; 13.8; 21.8 Dosage: 5 x 15 caps/ha chemical treatment l/ha chlorpyrifos, cypermethrin ,3 l/ha thiacloprid,3 l/ha thiacloprid,3 l/ha thiacloprid,3 l/ha thiacloprid The time of application,3 l/ha thiacloprid Date of evaluation 8.9.
19 iocont Laboratory Effect of on the cotton bollworm in pepper open field Locality: Želiezovce, SK, 23 Dosage: 5 x 15 caps/ha Date of treatment: 4.7; 9.7; 16.7; 13.8; 21.8 Date of evaluation: 8.9. Evaluated sample: 8 x 1 fruits 15 14,1 % Infestation % 1 5 3,1 % chemical treatment
20 pieces. iocont Laboratory Crop: Pepper open field Locality: Marcelová, SK, 23 Pest: Cotton bollworm (H. armigera) Date of treatment: 4.7; 9.7; 16.7 Dosage: 3 x 15 caps/ha chemical treatment ,2 l/ha thiacloprid The time of application ,2 l/ha thiacloprid ,2 l/ha thiacloprid 4.8. Date of evaluation
21 iocont Laboratory Effect of on the cotton bollworm in pepper open field Locality: Marcelová, SK, 23 Dosage: 3 x 15 caps/ha Date of treatment: 4.7; 9.7; 16.7 Date of evaluation: 4.8. Evaluated sample: 6 x 1 fruits 1 8 Infestation % ,7 % 2,7 % chemical treatment
22 iocont Laboratory Crop: Pepper open field Locality: Marcelová, SK, 23 Pest: Cotton bollworm (H. armigera) Date of treatment:13.8; 21.8 Dosage: 2 x 15 caps/ha 3 25 The time of application Date of evaluation pieces
23 iocont Laboratory Effect of on the cotton bollworm in pepper open field Locality: Marcelová, SK, 23 Evaluated sample: 1x1 plants Date of evaluation: 9.9. Efficacy: 76 % Dosage: 2x15 caps/ha , % infestation % ,8 % control
24 iocont Laboratory pieces Crop: Tobacco Locality: átorové Kosihy, SK, 23 Pest: Cotton bollworm (H. armigera) Date of treatment: : 4.7; 9.7; x. thuringiensis 3l/ha 11.7 Dosage: 3 x 1 cap/ha chemical treatment 11.7.,1 l/ha bifenthrin 5 The time of application l / ha. thuringiensis Date of evaluation
25 iocont Laboratory Effect of on the cotton bollworm in tobacco Locality: átorové Kosihy, SK, 23 Evaluated sample: 4x1 plants Dosage: 3 x 1 cap/ha,. thuringiensis 1 x 3 l / ha Date of evaluation: 5.8. % infestation thuringiensis. thuringiensis chemical treatment
26 iocont Laboratory dvantages of the capsules hitch capsules on plant ensures optimal conditions during the whole hatching of Trichogramma
27 iocont Laboratory dvantages of the capsules contains the biological product of the top quality efficient protection of beneficial wasps against adverse effect of climate and predators advisory support for correct timing of delivery and application is included in the price up to 8 % efficacy is reached provided that all recommendations for use are followed hitch capsules on plant ensures optimal conditions during the whole hatching of Trichogramma
28 iocont Laboratory Ltd. Šmahova 66, rno Slatina, 627 Czech Republic Phone: biocont@biocont.cz www. biocont.cz hluchy@biocont.cz
29 iocont Laboratory The cabbage moths (Mamestra brassicae)
30 iocont Laboratory The greatest damage is usually caused by the larvae of the second generation, which are often more numerous than the first generation.
31 iocont Laboratory Dosage: Mamestra brassicae cabbage 24 x 1 caps/gen/ha
32 iocont Laboratory The flight activity of cabbage moths The right time of application 1st application 2nd application 3nd application May June July ugust September F2 F1 P parental generation F1, F2 filial generation P
33 pieces. iocont Laboratory Crop: White cabbage Locality: Otice, CZ, 23 Pest: Cabbage moth (M.brassicae), Cabbage worm (P.rapae) Date of treatment: 15.7; 22.7; 31.7; 5.8 Dosage: 4 x 1 caps / ha chemical treatment ,2 l / ha thiacloprid,6 l / ha chlorpyrifos + cypermethrin The time of application Date of evaluation
34 iocont Laboratory Effect of on the cabbage moth in cabbage Locality: Otice, CZ, 23 Dosage: 4 x 1 caps/ha Evaluated sample: 4 x 5 plants Date of evaluation: 26.8 Efficacy : 83,9 % chemical treatment 98,4 % Infestation % chemical treatment control
35 iocont Laboratory Crop: White cabbage Locality: Gy r, Hungary, 22 Pest: Cabbage moth (M.brassicae), Cabbage worm (P.rapae) Date of treatment: 5.7; 11.7; 19.7; 29.7 Dosage: 4 x 1 caps / ha 5 4 The time of application Date of evaluation pieces
36 iocont Laboratory Effect of on the cabbage moth in cabbage Locality: Gy r, Hungary, 22 Dosage: 4 x 1 caps/ha Date of evaluation: 5.8. Evaluated sample: 4 x 5 plants Efficacy: Mamestra brassicae 1 % Pieris rapae 44 % 8 Number of larvae/ 2 plant control Mamestra brassicae control Pieris rapae Source SP
37 iocont Laboratory dvantages of the capsules hitch capsules on plant ensures optimal conditions during the whole hatching of Trichogramma
38 iocont Laboratory dvantages of the capsules contains the biological product of the top quality efficient protection of beneficial wasps against adverse effect of climate and predators advisory support for correct timing of delivery and application is included in the price up to 8 % efficacy is reached provided that all recommendations for use are followed hitch capsules on plant ensures optimal conditions during the whole hatching of Trichogramma
39 iocont Laboratory Ltd. Šmahova 66, rno Slatina, 627 Czech Republic Phone: biocont@biocont.cz www. biocont.cz hluchy@biocont.cz
40 Tab. No. 1 Crop Greenhouse G Field F Locality Country Year Dosage (specimen s per hectare) Evaluated sample Infestatio n on the control (caterpilla r/sample) Infestatio n Trichoplu s Plot (caterpilla r/sample) Infestation chemical protection (caterpillar /sample) Efficacy % Efficacy chemical protection % Green bean F Nagyherkály puszta HU 22 3 x x 1 pods ,2 Tomato G Tordas HU 22 2 x 2. 3 x 2. 4 x 5 fruits 4 x 5 fruits ,8 82,3 Sweet pepper G Tordas HU 22 2 x 2. 3 x 2. 4 x 5 fruits 4 x 5 fruits ,9 77,3 Cabbage F Rábakecöl HU 22 4 x 1. 4 x 5 plants 12 1, Cabbage F Otice CZ 23 4 x x 5 plants ,7 98,
41 Tab. No. 2 Crop Greenhouse G Field F Locality Country Year Dosage (specimens per hectare) Evaluated sample Infestation on the control (infected/ not infected) Infestation (infected/ not infected Infestation chemical protection (infected/ not infected Efficacy % Efficacy chemical protection % Tomato F Vrbová/V. SK 23 Tab. No.3 3 x x 3. fruits on 4 x 1 plants 535/65 128/ /37 83,4 46,9 Sweet pepper F Marcelov SK 23 2 x 3. 1 x 1 plants 116/465 5/1 76, Tab. No.3 Crop Field F Locality Country Year Dosage (specimens per hectare) Evaluated sample Infestation infected/not infected Infestation chemical protection infected/not infected Infestation % Infestation chemical protection % Tomato F Želiezovce SK 23 3 x x 3. 4 x 1 plants 168/ /168 11,4 25,9 Sweet pepper F Želiezovce SK 23 3 x x 3. 8 x 1 plants 23/78 81/492 3,1 14,1 Sweet pepper F Marcelová SK 23 3 x x 1 plants 13/477 12/434 2,7 2,7 Tab. No.4 Crop Field F Locality Year Country Dosage (specimens per hectare) Evaluated sample Infestation + iobit infected/ not infected Infestation iobit infected/ not infected Infestation chemical protection infected/ not infected Infestation + iobit % Infestation iobit % Infestation chemical protection % Tobacco F. Kosihy SK 23 2 x x x 1 plants /4 7/33 34/6 % 17,5 % 85 %
42 Tab. No. 5 Numbe rof trial Crop Locality Evaluated sample Variation Infestation (caterpillar/sample) Repetition Statistical significance 9 % 95 % 99 % 1 Green bean Nagyherkály puszta 4 x 1 pods control Tomato Tordas 1. evaluation 4 x 5 fruits control evaluation 4 x 5 fruits control Sweet pepper Tordas 1. evaluation 4 x 5 fruits control evaluation 4 x 5 fruits control Cabbage Rábakecöl 4 x 5 plants control
43 Tab. No. 6 Numb er of trial Crop Locality Evaluated sample Variation Infestation ( infected / not infected) Repetition Statistical significance 9 % 95 % 99 % 5 Tomato Vrbová/V. Fruit on 1 x 1 plants control 46/6 15/73 68/5 16/85 49/6 13/69 65/7 19/8 2 59/6 14/79 47/9 1/63 44/7 11/77 69/4 13/81 48/7 11/66 4/8 6/6 chemical treatment 32/39 25/36 39/28 25/2 2 15/21 16/28 29/36 38/39 42/32 15/26 C C C 6 Sweet pepper Marcelová 1 x 1 plants control 22/18 7/48 12/4 15/5 7 8/58 15/51 9/65 6/51 6/45 16/32 9/54 7/118 3/18 2/83 4/71 6/131 4/134 5/13 4/96 6/12 7 Cabbage Otice 4 x 5 plants control 6/44 17/33 13/37 13/3 7 2/48 2/48 3/47 1/49 chemical treatment /5 1/49 /5 /5 C C 8 Tomato Želiezovce 3 x 1 plants 56/311 6/372 52/623 chemical treatment 14/ / /535 9 Sweet pepper Želiezovce 8 x 1 plants chemical treatment 1/53 6/92 4/59 8/67 7/93 8/56 3/97 3/45 4/19 22/28 /96 18/63 1/99 9/7 3/12 7/71 1 Sweet pepper Marcelová 6 x 1 plants chemical treatment 2/75 1/73 3/76 2/79 1/67 2/87 3/89 3/92 3/92 3/69 1/78 1/34 11 Tobacco. Kosihy 4 x 1 plants + iobit /1 /1 /1 /1 iobit 2/8 1/9 1/9 3/7 chemical treatment 8/2 9/1 9/1 8/2 C C C
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