Effects of TBCO on the Reproductive Capacity of 2 Generations of Japanese Medaka (Oryzias latipes)
|
|
|
- Roland Evans
- 5 years ago
- Views:
Transcription
1 Effects of on the Reproductive Capacity of 2 Generations of Japanese Medaka (Oryzias latipes) Br Br Br Br Steve Wiseman University of Saskatchewan
2 Multigenerational Effects Pesticides Vinclozolin (anti-androgen) (multigenerational) (all generations exposed) 4 generations of males from vinclozolin-treated females show a higher level of testis apoptosis. (transgenerational) (no direct exposure)
3 Generational Effects in Fish BPA EE2 No effects in F and F1 generations Reduced fertilization in F2 generation Reduced embryo-survival in F3 generation
4 Are there Multigenerational Effects of? Saunders, D.M.V., M. Podaima, S. Wiseman, J.P. Giesy Effects of the Brominated Flame Retardant on Title Fecundity or place and of presentation Profiles of Transcripts of the HPGL-Axis in Japanese Medaka. Aquatic Toxicol. 16:
5 1,2,5,6-Tetrabromocyclooctane () Br Br Hexobromocyclododecane (HBCD) Br Br Thermal insulation foams Textile coatings Meets EU criteria aquatic hazard Not detected in the environment EDC in vitro Disruption of sex- steroid receptor signaling Disruption of steroidogenesis 21: 28, tons/yr Being phased out (some exceptions) Replacements being assessed.
6 1. Effects of Dietary Exposure to on Reproductive Capacity of F Generation Japanese Medaka Saunders, D.M.V., M. Podaima, S. Wiseman, J.P. Giesy Effects of the Brominated Flame Retardant on Title Fecundity or place and of presentation Profiles of Transcripts of the HPGL-Axis in Japanese Medaka. Aquatic Toxicol. 16:
7 Cumulative Egg Production Egg Number Control Time (Days)
8 Cumulative Egg Production Egg Number Control - 58 ug/g food Time (Days) Decreased fecundity
9 Cumulative Egg Production Egg Number Control - 67 ug/g food - 58 ug/g food Time (Days) Decreased fecundity caused only by lowest dose of
10 Maternal Transfer of 25 Egg [] ug/g BDL Ctrl - 58 ug/g - 67 ug/g Dietary []
11 2. Effects of Maternally Transferred on Reproductive Capacity of F1 Generation
12 Question: Does maternally deposited affect reproductive capacity? Control Maternal
13 Question: Does maternally deposited affect reproductive capacity? Control Freshwater Maternal Freshwater
14 Question: Does maternally deposited affect reproductive capacity? Control Freshwater Control Diet Ctrl / Ctrl Maternal Freshwater Control Diet / Ctrl
15 Cumulative Egg Production (F1) 12 1 Ctrl-Ctrl Egg Number Time (Days)
16 Cumulative Egg Production (F1) Egg Number Ctrl-Ctrl -Ctrl Decreased fecundity caused by exposure to maternal Time (Days)
17 Question: What is the effect of re-exposure to? Control Freshwater Control Diet Ctrl / Ctrl Maternal Freshwater Control Diet / Ctrl
18 Question: What is the effect of re-exposure to at sexual maturity. Control Diet Ctrl / Ctrl Control Freshwater Dietary Ctrl / Maternal Freshwater Control Diet / Ctrl Dietary /
19 Cumulative Egg Production (F1) Egg Number Ctrl-Ctrl -Ctrl Time (Days)
20 Cumulative Egg Production (F1) Egg Number Ctrl-Ctrl Ctrl- -Ctrl Decreased fecundity caused by exposure to Time (Days)
21 Cumulative Egg Production (F1) Egg Number Ctrl-Ctrl Ctrl- -Ctrl - Re-exposure to during reproduction ameliorates effect of maternal Time (Days)
22 3. Mechanism of Effects Epigenetic? Methylome is erased and re-established during early development Chemical stressors can alter the methylome Changes in DNA methylation are mitotically heritable Hypermethylation decreased expression (transcriptional silencing) Hypomethylation increased expression (transcriptional activation)
23 Embryo-Exposure 6 Day (1hpf) Freshwater 1 ug/l (Hatch) Conc. in Embryo (ug/g) y = x R² = Days
24 Question: Does maternally deposited affect reproductive capacity? Freshwater
25 Question: Does maternally deposited affect reproductive capacity? Freshwater Freshwater Freshwater
26 Question: What is the effect of re-exposure to at sexual maturity. Control Diet Ctrl / Ctrl Freshwater Freshwater Dietary Ctrl / Freshwater Control Diet / Ctrl Dietary /
27 Candidate Gene Approach Female Fry Vtg II * ER-β Ctrl Ctrl CHG-H * AR * Ctrl Ctrl Greater expression of genes for sex steroid signalling
28 Candidate Gene Approach Female Fry 1.5 StAR 1.5 Cyp19a (aromatase) *.5 * Ctrl Ctrl Lesser expression of genes for sex steroid synthesis.
29 Candidate Gene Approach Female Liver 1.2 VTG II 1.5 CHG-H.8.4 * * 1.5 * * 1.5 ERa 1.5 * * Lesser expression of genes for sex steroid signalling.
30 Candidate Gene Approach Female Gonad 2.5 CYP19a * * 4 StAR 3 * 2 1 * * Greater expression of genes for sex steroid synthesis.
31 Conclusions Dietary exposure to lowers fecundity of F female medaka. Maternally deposited lowers fecundity of F1 medaka However, fecundity of F1 fish exposed to maternally deposited is attenuated by re-exposure to dietary during reproduction. Predictive adaptive response? Compared to F fish, female F1 fish exposed to during ELS have altered basal expression of key genes of the reproductive axis. Altered methylation/epigenetic effect? Effects on F2?
32 David Saunders (PhD Student) Jianxian Sun (PDF) Hui Peng (PDF) Song Tang (PDF) Jeremy Kuzma (BSc Student) Markus Hecker John Giesy
Aquatic Toxicology 160 (2015) Contents lists available at ScienceDirect. Aquatic Toxicology
Aquatic Toxicology 160 (2015) 180 187 Contents lists available at ScienceDirect Aquatic Toxicology journal homepage: www.elsevier.com/locate/aquatox Effects of the brominated flame retardant TBCO on fecundity
AGREEMENT OF THE MEMBER STATE COMMITTEE ON THE IDENTIFICATION OF 4,4'-ISOPROPYLIDENEDIPHENOL (BISPHENOL A) AS A SUBSTANCE OF VERY HIGH CONCERN
AGREEMENT OF THE MEMBER STATE COMMITTEE ON THE IDENTIFICATION OF 4,4'-ISOPROPYLIDENEDIPHENOL (BISPHENOL A) AS A SUBSTANCE OF VERY HIGH CONCERN According to Articles 57 and 59 of Regulation (EC) 1907/2006
Received: March 13, 2016 Revised: June 15, 2016 Accepted: June 20, 2016 Published: June 20, 2016
Article pubs.acs.org/est Combined Transcriptomic and Proteomic Approach to Identify Toxicity Pathways in Early Life Stages of Japanese Medaka (Oryzias latipes) Exposed to 1,2,5,6-Tetrabromocyclooctane
DOHaD: Role of environmental chemical exposures
DOHaD: Role of environmental chemical exposures Linda S. Birnbaum, Ph.D., D.A.B.T., A.T.S. Director, National Institute of Environmental Health Sciences and National Toxicology Program PPTox-IV Boston,
(Received 20 February 2008; Accepted 21 May 2008)
Environmental Toxicology and Chemistry, Vol. 27, No. 12, pp. 2504 2511, 2008 2008 SETAC Printed in the USA 0730-7268/08 $12.00.00 TIME-DEPENDENT TRANSCRIPTIONAL PROFILES OF GENES OF THE HYPOTHALAMIC-PITUITARY-GONADAL
Towards 2020: Linda S. Birnbaum, Ph.D., D.A.B.T., A.T.S. Director National Institute of Environmental Health Sciences National Toxicology Program
Towards 2020: What are the Critical Environmental Health Challenges? Linda S. Birnbaum, Ph.D., D.A.B.T., A.T.S. Director National Institute of Environmental Health Sciences National Toxicology Program
Classification of developmentally toxic pesticides, low dose effects, mixtures perspective of the industry
Classification of developmentally toxic pesticides, low dose effects, mixtures perspective of the industry Steffen Schneider 9th Berlin Workshop on Developmental Toxicology Berlin September 13, 2018 What
Exposures and Heritable Health Effects
Exposures and Heritable Health Effects Thaddeus T. Schug Population Health Branch National Institute of Environmental Health Sciences November 30, 2017 Outline Developmental exposures and health effects
ASSESSMENT OF THE SENSITIVITY OF NORTH AMERICAN FISH SPECIES TO ENDOCRINE DISRUPTING CHEMICALS IN VITRO
ASSESSMENT OF THE SENSITIVITY OF NORTH AMERICAN FISH SPECIES TO ENDOCRINE DISRUPTING CHEMICALS IN VITRO A Thesis Submitted to the College of Graduate Studies and Research In Partial Fulfillment of the
A Testing Strategy for the Identification of mammalian Endocrine Disruptors with particular focus on steroids
A Testing Strategy for the Identification of mammalian Endocrine Disruptors with particular focus on steroids Tzutzuy Ramirez, Robert Landsiedel, Susanne Kolle, Hennicke Kamp, Bennard van Ravenzwaay EUSAAT
Assessment and regulation of endocrine disrupters under European chemical legislations Susy Brescia Chemicals Regulation Directorate (CRD) HSE
Health and and Safety Executive Assessment and regulation of endocrine disrupters under European chemical legislations Susy Brescia Chemicals Regulation Directorate (CRD) HSE EDs and the current EU legislative
Sex Determination and Gonadal Sex Differentiation in Fish
Sex Determination and Gonadal Sex Differentiation in Fish Yoshitaka Nagahama Okazaki National Research Institutes, Japan This first slide shows the processes of gonadal sex differentiation and gametogenesis
The intra-follicular molecular biology mandating advancement of egg retrieval in some women
The intra-follicular molecular biology mandating advancement of egg retrieval in some women David H. Barad, USA Director of Assisted Reproductive Technology, The Center for Human Reproduction New York
Effects of weathered oil and dispersants on hatchling morphology, apical and molecular endpoints of Menidia beryllina embryo
Effects of weathered oil and dispersants on hatchling morphology, apical and molecular endpoints of Menidia beryllina embryo Adeyemo, OK, Kroll, KJ, Garcia-Reyero, N, Denslow, ND OUTLINE Introduction Experimental
E nvironmental chemicals can induce a variety of adverse effects via disruption of normal endocrine function
OPEN SUBJECT AREAS: ENDOCRINE REPRODUCTIVE DISORDERS ANIMAL PHYSIOLOGY Received 30 November 2014 Accepted 25 February 2015 Published 20 March 2015 Correspondence and requests for materials should be addressed
Estrogen receptor beta regulates locus-specific DNA methylation a possible mechanism for epigenetic effects of endocrine disrupters
Estrogen receptor beta regulates locus-specific DNA methylation a possible mechanism for epigenetic effects of endocrine disrupters Joëlle Rüegg Swedish Toxicology Science Research Center, Swetox Karolinska
Responses of the Medaka HPG Axis PCR Array and Reproduction to Prochloraz and Ketoconazole
Environ. Sci. Technol. 2008, 42, 6762 6769 Responses of the Medaka HPG Axis PCR Array and Reproduction to Prochloraz and Ketoconazole XIAOWEI ZHANG,*,, MARKUS HECKER,,, PAUL D. JONES,, JOHN NEWSTED,, DORIS
"From field to lab" and "from lab to field
"From field to lab" and "from lab to field chemicals, in vitro, in vivo vs. in situ Luděk Bláha 1, Klára Hilscherová 1, Edita Mazurová 1, John P. Giesy 2, Rita Triebskorn 3, Ivan Holoubek 1 RECETOX Research
I) Development: tissue differentiation and timing II) Whole Chromosome Regulation
Epigenesis: Gene Regulation Epigenesis : Gene Regulation I) Development: tissue differentiation and timing II) Whole Chromosome Regulation (X chromosome inactivation or Lyonization) III) Regulation during
Allelic reprogramming of the histone modification H3K4me3 in early mammalian development
Allelic reprogramming of the histone modification H3K4me3 in early mammalian development 张戈 Method and material STAR ChIP seq (small-scale TELP-assisted rapid ChIP seq) 200 mouse embryonic stem cells PWK/PhJ
ISRTP Workshop. Cumulative Risk for Endocrine Disrupters: The Case FOR a dose addition approach. Andreas Kortenkamp
ISRTP Workshop Cumulative Risk for Endocrine Disrupters: The Case FOR a dose addition approach Andreas Kortenkamp The School of Pharmacy, University of London 19-20 February 2008 Endocrine disruption:
Effects of Pharmaceuticals in the Canadian Environment
Effects of Pharmaceuticals in the Canadian Environment Joanne Parrott, Science and Technology Branch, Environment Canada, 867 Lakeshore Road, Burlington, Ontario L7R 4A6 Why Worry about Pharmaceuticals?
Availability of R eceptor B inding A ssay for Screening of E ndocrine D isrupting C hemicals
Session II. Endocrine Disrupting Effects and Test Methods with medaka : Part I Availability of R eceptor B inding A ssay for Screening of E ndocrine D isrupting C hemicals Makoto Nakai Chemicals Assessment
Leif Norrgren. Department of Biomedicine and Veterinary Public Health Faculty of Veterinary Medicine and Animal Health SLU, Uppsala, Sweden
Fish Models for Ecotoxicology Leif Norrgren Department of Biomedicine and Veterinary Public Health Faculty of Veterinary Medicine and Animal Health SLU, Uppsala, Sweden Acknowledgements Patrik Andersson
Untargeted strategy for identification of Toxic Chemicals in OSPW
Untargeted strategy for identification of Toxic Chemicals in OSPW ---- oxidative stress and PPARG agonistic activity in OSPW Jianxian Sun, Hui Peng, Hattan Al Harbi, John P. Giesy, Steve Wiseman Oct 6,
Environmental and Developmental Origins of Ovarian Reserve. Nick Macklon Professor of Obstetrics and Gynaecology, University of Southampton, UK.
Environmental and Developmental Origins of Ovarian Reserve Nick Macklon Professor of Obstetrics and Gynaecology, University of Southampton, UK. Why worry? Women delaying childbirth Diminished ovarian
Intersex in Bass Emerging Contaminant Issues
Intersex in Bass Emerging Contaminant Issues Vicki S. Blazer U.S Geological Survey National Fish Health Research Laboratory Leetown Science Center, Kearneysville, WV Sampling With WV DNR u Spring Summer
Sex differentiation of germ cells in the teleost, Oryzias latipes, during normal embryonic development
J. Embryol. exp. Morph. Vol. 2, 2, pp. -, 2 Printed in Great Britain Sex differentiation of germ cells in the teleost, Oryzias latipes, during normal embryonic development ByNORIYUKI SATOH AND NOBUO EGAMI
Early Exposure Late Effects in Invertebrates and Vertebrates
Early Exposure Late Effects in Invertebrates and Vertebrates Society of Environmental Toxicology and Chemistry (SETAC-Europe) 16 th Annual Meeting The Hague, The Netherlands May 7-11, 2006 John P. Giesy
Hormone Disrupting Chemicals
Hormone Disrupting Chemicals Sperm cells 10 6 per ml 150 100 50 0 1930 1940 1950 1960 1970 1980 1990 2000 Intersex Intersex in British Perch populations Jobling et al. 1998 Intersex Roach (Jobling et
Mechanisms underlying epigene2c effects of endocrine disrup2ve chemicals. Joëlle Rüegg
Mechanisms underlying epigene2c effects of endocrine disrup2ve chemicals Joëlle Rüegg 2 EDCs and human health Bisphenol A Epigenetic mechanisms Behavioural and psychiatric disorders Phthalates DNA methylation
Setting the scene: Is mixtures risk assessment necessary and feasible?
3 rd SETAC Special Science Symposium Setting the scene: Is mixtures risk assessment necessary and feasible? Andreas Kortenkamp The School of Pharmacy, University of London 2-3 February 2011, Brussels The
Estrogen-Independent Ovary Formation in the Medaka Fish, Oryzias latipes
Estrogen-Independent Ovary Formation in the Medaka Fish, Oryzias latipes Author(s): Tetsuya Kawahara and Ichiro Yamashita Source: Zoological Science, 17(1):65-68. Published By: Zoological Society of Japan
Transcriptional Responses of the Brain Gonad Liver Axis of Fathead Minnows Exposed to Untreated and Ozone-Treated Oil Sands Process-Affected Water
pubs.acs.org/est Transcriptional Responses of the Brain Gonad Liver Axis of Fathead Minnows Exposed to Untreated and Ozone-Treated Oil Sands Process-Affected Water Yuhe He, Steve B. Wiseman,*, Nan Wang,
Chapter 11 Environmental Epigenetics and Epigenetic Transgenerational Inheritance
Chapter 11 Environmental Epigenetics and Epigenetic Transgenerational Inheritance Michael K. Skinner Abstract The developing role of environmental epigenetics in biology and medicine is discussed in relationship
Session 2: Biomarkers of epigenetic changes and their applicability to genetic toxicology
Session 2: Biomarkers of epigenetic changes and their applicability to genetic toxicology Bhaskar Gollapudi, Ph.D The Dow Chemical Company Workshop: Genetic Toxicology: Opportunities to Integrate New Approaches
Endocrine Disrupters as Obesogens
Endocrine Disrupters as Obesogens Is the environment making us fat? Bruce Blumberg, Ph.D. Department of Developmental and Cell Biology Department of Pharmaceutical Sciences Developmental Biology Center
Hormone Disrupting Chemicals 1993: Sharpe and Skakkebæk publicerer østrogen hypotesen
Hormone Disrupting Chemicals 1993: Sharpe and Skakkebæk publicerer østrogen hypotesen We argue that the increasing incidence of reproductive abnormalities in the human male may be related to increased
pesticides in the EU regulatory framework Prof Andreas Kortenkamp
Addressing combined effects of pesticides in the EU regulatory framework Prof Andreas Kortenkamp Brunel University London Info session on cumulative risk assessment for o sess o o cu u at e s assess e
Sensitive and vulnerable risks groups in relation to chemical risk assessment and management
International Research and Research Training Centre Sensitive and vulnerable risks groups in relation to chemical risk assessment and management Niels E. Skakkebaek Department of Growth and Reproduction
Examining environmental contributors to autism spectrum disorder
Examining environmental contributors to autism spectrum disorder Valerie Hu, Ph.D. Dept. of Biochemistry and Molecular Medicine School of Medicine and Health Sciences The George Washington University E-mail:
Endocrine Disruptors, Crustacean Molting and Bivalve Populations. Peter L. defur Virginia Commonwealth University Richmond VA
Endocrine Disruptors, Crustacean Molting and Bivalve Populations Peter L. defur Virginia Commonwealth University Richmond VA Not so long ago, and not so far away We are certain of the following: a large
Hypothalamus, pituitary, and subordinate glands
Endocrine System The endocrine system is the body s slow communication system relying on three main components Endocrine glands, such as hypothalamus, pituitary, thyroid, adrenal, pancreas, gonad Hormones
All Mixed Up: Phenotypic Plasticity in a Genotypic World
All Mixed Up: Phenotypic Plasticity in a Genotypic World Amber Tompsett, Steve Wiseman, ric Higley, Sara Pryce, John P. Giesy, and Markus Hecker STAC urope Annual Meeting and World Congress May 21, 2012
An epigenetic approach to understanding (and predicting?) environmental effects on gene expression
www.collaslab.com An epigenetic approach to understanding (and predicting?) environmental effects on gene expression Philippe Collas University of Oslo Institute of Basic Medical Sciences Stem Cell Epigenetics
BROMINATED FLAME RETARDANTS (BFRs)
BROMINATED FLAME RETARDANTS (BFRs) Jana Hajšlová, Radek Kazda, Jan Poustka Institute of Chemical Technology, Prague Department of Food Chemistry and Analysis The 1st Workshop - Persistent Toxic Substances
10/18/2016. Endocrine Disruption and the Environment. Endocrine Policy Forum US EPA S LEGISLATIVE MANDATES (1996)
Endocrine Disruption and the Environment Ellen Mihaich, Ph.D., DABT PCPC Environmental Safety Workshop October 25, 2016 1 Endocrine Policy Forum Consortium of List 1 test order recipients and other interested/involved
Fish models for environmental and biomedical research
Fish models for environmental and biomedical research Peter Thomas Marine Science 1. Hypoxia Hypoxia state of reduced oxygen availability Occurs in: Marine and freshwater environments (aquatic hypoxia)
INTERSEX STURGEON IN THE MISSOURI RIVER? - THE EFFECTS OF EMERGING CONTAMINANTS ON A BIG RIVER
INTERSEX STURGEON IN THE MISSOURI RIVER? - THE EFFECTS OF EMERGING CONTAMINANTS ON A BIG RIVER Shovelnose sturgeon -Scaphirhynchus platorynchus Pallid sturgeon -Scaphirhynchus albus Photo courtesy of Aaron
Role of CpG deserts in the epigenetic transgenerational inheritance of differential DNA methylation regions
Skinner and Guerrero-Bosagna BMC Genomics 2014, 15:692 RESEARCH ARTICLE Open Access Role of CpG deserts in the epigenetic transgenerational inheritance of differential DNA methylation regions Michael K
Endocrine Disruptors: Effects On Male And Female Reproductive Systems, Second Edition READ ONLINE
Endocrine Disruptors: Effects On Male And Female Reproductive Systems, Second Edition READ ONLINE could have significant health implications according to the State of the Science of Endocrine Disrupting
Phase-3 OECD 21-day Fish Screening Assay Validation Report Additional Test Substance Studies
Report of Eight 21-day Fish Endocrine Screening Assays With Additional Test Substances for Phase-3 of the OECD Validation Program: Studies with Octylphenol in the Fathead Minnow (Pimephales promelas) and
Chapter 16: Steroid Hormones (Lecture 17)
Chapter 16: Steroid Hormones (Lecture 17) A) 21 or fewer carbon atoms B) Precursor: 27 carbon cholesterol C) major classes of steroid hormones 1) progestagens a) progesterone- prepares lining of uterus
Joanna Hillman Michael Higgins Lab Oncology for Scientists I 10/29/2015
Joanna Hillman Michael Higgins Lab Oncology for Scientists I 10/29/2015 ! Define Epigenetics & Genomic Imprinting! Discovery! What is the imprint! Lifecycle of an Imprint DMRs and ICEs! 2 main mechanisms
Spermatogenesis. What is it and what does it look like? How do hormones regulate spermatogenesis?
Spermatogenesis What is it and what does it look like? How do hormones regulate spermatogenesis? FSH, androgens, growth factors Animal Physiology (Hill, Wise, Anderson): Ch. 15 435-438 1 Spermatogenesis:
Effects of pharmaceuticals on gene expression and hormone production in H295R cell line using QRT-PCR/ELISA
Effects of pharmaceuticals on gene expression and hormone production in H29R cell line using QRT-PCR/ELISA Tannia Gracia P. D. Jones, K. Hilscherova, M. Hecker, E. Higley, J. L. Newsted and J.P. Giesy
Animal Reproduction. Overview of Male Reproduction. # lectures for cumulative test # 01 book 01
Animal Reproduction JP Advis DVM, Ph.D. Bartlett Hall, Animal Sciences, Cook, (732) 932-9240, advis@aesop.rutgers.edu 04 Course website: rci.rutgers.edu/~advis Material to be covered: About lecture Meetings
3.D- Cell Communication
3.D- Cell Communication Big Idea 3: Living systems store, retrieve, transmit and respond to information essential to life processes. EU 3.A: Heritable information provides for continuity of life. EU 3.B:
Endocrine Disrupting Chemicals: New Tools for Assessing Ecological Risk
Endocrine Disrupting Chemicals: New Tools for Assessing Ecological Risk G. Ankley Office of Research and Development NHEERL, Mid-Continent Ecology Division, Duluth, MN February 25, 2009 What are Endocrine-Disrupting
This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and
This article appeared in a journal published by Elsevier. The attached copy is furnished to the author for internal non-commercial research and education use, including for instruction at the authors institution
Angeliki Lyssimachou, PhD Environmental Scientist/Toxicologist
Angeliki Lyssimachou, PhD Environmental Scientist/Toxicologist Pesticides a health concern Designed to be toxic to living organisms they pass through biological membranes May interact with hormones, their
GENDER James Bier
GENDER 2005-2008 James Bier Objectives 1. State the method of determining gender in several genetic systems. 2. List the three regions of the Y chromosome. 3. Describe the events that promote sexual development
Exploring Environmental Chemicals and Risk of Breast Cancer During the Menopausal Transition
Exploring Environmental Chemicals and Risk of Breast Cancer During the Menopausal Transition Susan L. Neuhausen, Ph.D Morris and Horowitz Families Endowed Professor Beckman Research Institute of City of
Endocrine Disrupting Chemicals. - according to REACH regulation
Endocrine Disrupting Chemicals - according to REACH regulation Lone Mikkelsen Policy officer Chemicals Copenhagen Chemicals Summit 29/9 2016 Agenda Absence of adequate regulation for endocrine disrupting
Swimming Upside-down: A Journey with Fish from Space to Cancer Research
S 2 DS 2 A 2015-02/27/2015 Swimming Upside-down: A Journey with Fish from Space to Cancer Research Maki Niihori The University of Arizona Ear Institute Department of Otolaryngology The University of Arizona
Topics for this lecture: Sex determination Sexual differentiation Sex differences in behavior and CNS development. 1) organizational effects of
Topics for this lecture: Sex determination Sexual differentiation Sex differences in behavior and CNS development. 1) organizational effects of gonadal steroids on CNS development 2) our model system:
The significance of genomic imprinting in assisted reproduction
Gynaecological Clinic Rigshospitalet Copenhagen University The significance of genomic imprinting in assisted reproduction Øjvind Lidegaard Øjvind Gynaecological Lidegaard Clinic Rigshospitalet Copenhagen,
Endocrine Disrupter Chemicals: Universal Access to Healthcare
Endocrine Disrupter Chemicals: the right to know Riana Bornman, School of Health Systems and Public Health, University of Pretoria SAMA Conference 2016 Universal Access to Healthcare Concern started SOS-EDCs
4/20/2016. Objectives. Epigenetic Definitions. Gene Expression. More Questions. Questions to Consider
Objectives Epigentics: You Might Be What Your Grandmother Ate Lynda Britton, Ph.D., MLS(ASCP) CM Professor LSU Health Shreveport Discuss epigenetics and its role in cancer, imprinting and X chromosome
Bioavailability of metals to fish: its molecular basis, toxicological consequences and how it can be assessed. Christer Hogstrand
Bioavailability of metals to fish: its molecular basis, toxicological consequences and how it can be assessed Christer Hogstrand Metal Metabolism Group King s College London Global maps for the year 2000
Genetics and Genomics in Medicine Chapter 6 Questions
Genetics and Genomics in Medicine Chapter 6 Questions Multiple Choice Questions Question 6.1 With respect to the interconversion between open and condensed chromatin shown below: Which of the directions
CHAPTER 1. INTRODUCTION. Along with growth and spawning season, year at sexual maturity counts as another
CHAPTER 1. INTRODUCTION 1.1 Year at Sexual Maturity in Rainbow Trout Along with growth and spawning season, year at sexual maturity counts as another important trait in farming rainbow trout. Year at sexual
Frederick vom Saal Division of Biological Sciences University of Missouri-Columbia, USA
INTERNATIONAL SEMINARS ON PLANETARY EMERGENCIES 49th Session SCIENCE FOR PEACE THE WORLD OVER (THE NEW MANHATTAN PROJECT) Erice 20-23 August 2016 SESSION 8: CHILDREN WELFARE DISRUPTION OF DEVELOPMENT BY
WP 3 : REPRODUCTION & GENETICS Matura7on and spawning induc7on of Grey Mullet (Mugil cephalus)
New species for EU aquaculture WP 3 : REPRODUCTION & GENETICS Matura7on and spawning induc7on of Grey Mullet (Mugil cephalus) Hanna Rosenfeld, Vered Zlatnikov, Chen Bracha, Eldad Toledano, Iris Meiri-Ashkenazi
ALMA AQUACULTURE RESEARCH STATION University of Guelph, Office of Research
ALMA AQUACULTURE RESEARCH STATION University of Guelph, Office of Research RESEARCH HIGHLIGHTS 2007 2008 Project ARS 120 - Investigating the Radiation Bystander Effect in Fish. CONTACT INFORMATION: Aquaculture
Under which legislation was the information requested (Regulation 793/93 or Directive 67/548/EEC): Regulation 793/93
1 (16) Conclusion of substance evaluation for transitional dossiers Summary conclusion of evaluation of a substance according to transitional measures described in Article 135(2), 136 (1) or 136(2) and
4/8/2016. Objectives. Epigenetic Definitions. Gene Expression. More Questions. Epigentics. Questions to Consider
Objectives Epigentics Lynda Britton, Ph.D., MLS(ASCP) CM Professor LSU Health Shreveport Discuss epigenetics and its role in cancer, imprinting and X chromosome inactivation. Describe the modifications/mechanisms
BEHAVIORAL AND NEURAL EFFECTS IN QUAIL EXPOSED TO XENOESTROGENS DURING EMBRYONIC DEVELOPMENT
BEHAVIORAL AND NEURAL EFFECTS IN QUAIL EXPOSED TO XENOESTROGENS DURING EMBRYONIC DEVELOPMENT Panzica G.C.*, Mura E., Viglietti-Panzica C.* UNIVERSITY OF TORINO, ITALY Dept. Anatomy, Pharmacology and Forensic
Table of Validated and Accepted Alternative Methods
1 de 8 25/10/2011 13:02 Non-animal Methods for Toxicity Testing Table of Validated and Accepted Alternative Methods Last Updated: September 27, 2011 Validation & Status of Alternative Test Methods & Testing
Knotting Nets: Molecular Junctions of Interconnecting Endocrine Axes Identified by Application of the Adverse Outcome Pathway Concept
Environmental Toxicology and Chemistry Volume 37, Number 2 pp. 318 328, 2018 318 Received: 30 March 2017 Revised: 12 May 2017 Accepted: 3 October 2017 Critical Review Knotting Nets: Molecular Junctions
Psychoactive drugs: Occurrence in aquatic environment, analytical methods and ecotoxicity a review
Psychoactive drugs: Occurrence in aquatic environment, analytical methods and ecotoxicity a review Deivisson Lopes Cunha 1,2, Frederico Goytacazes de Araujo 1,3 & Marcia Marques 1* 1 Dep. of Sanitary and
Imprinting. Joyce Ohm Cancer Genetics and Genomics CGP-L2-319 x8821
Imprinting Joyce Ohm Cancer Genetics and Genomics CGP-L2-319 x8821 Learning Objectives 1. To understand the basic concepts of genomic imprinting Genomic imprinting is an epigenetic phenomenon that causes
Kazunori Fujii. National Research Institute of Fisheries and Environment of Inland Sea, Fisheries Research Agency
The Effects of Endocrine Disruptors on Fish Maturation and Reproduction - A Focus on Projects Underway at the Ministry of Agriculture, Forestry, and Fisheries - Kazunori Fujii National Research Institute
Low-dose effects, non-monotonic dose responses and endocrine disrupting chemicals
Low-dose effects, non-monotonic dose responses and endocrine disrupting chemicals J.P. Myers, Ph.D. Charlottesville, VA Carnegie Mellon University Pittsburgh, PA H. John Heinz III Center for Science, Economics
OECD GUIDELINE FOR THE TESTING OF CHEMICALS. Fish Short Term Reproduction Assay. Replaced
OECD/OCDE 229 Adopted: 7 September 2009 OECD GUIDELINE FOR THE TESTING OF CHEMICALS INTRODUCTION Fish Short Term Reproduction Assay 1. The need to develop and validate a fish assay capable of detecting
Female Reproductive Physiology. Dr Raelia Lew CREI, FRANZCOG, PhD, MMed, MBBS Fertility Specialist, Melbourne IVF
Female Reproductive Physiology Dr Raelia Lew CREI, FRANZCOG, PhD, MMed, MBBS Fertility Specialist, Melbourne IVF REFERENCE Lew, R, Natural History of ovarian function including assessment of ovarian reserve
ENDOCRINE-DISRUPTING CHEMICALS IN THE EUROPEAN UNION
ENDOCRINE-DISRUPTING CHEMICALS IN THE EUROPEAN UNION INTRODUCTION Endocrine-disrupting chemicals (EDCs) are chemicals, or mixtures of chemicals, that interfere with any aspect of hormone action. These
Endocrine Disrupting Chemicals: New considerations in the toxics space TURI Annual Meeting
Endocrine Disrupting Chemicals: New considerations in the toxics space TURI Annual Meeting Laura N. Vandenberg, PhD UMass Amherst School of Public Health April 25, 2018 Disclosure statement I am funded
REPRODUCTIVE ENDOCRINOLOGY OF THE MALE
Reproductive Biotechnologies Andrology I REPRODUCTIVE ENDOCRINOLOGY OF THE MALE Prof. Alberto Contri REPRODUCTIVE ENDOCRINOLOGY OF THE MALE SPERMATOGENESIS AND REPRODUCTIVE BEHAVIOR RELATED TO THE ACTIVITY
An integrated approach to studying in vitro, clinical and epidemiological effects of endocrine disruptor exposure prenatally and in early infancy
RTP North Carolina, March 2, 2015 An integrated approach to studying in vitro, clinical and epidemiological effects of endocrine disruptor exposure prenatally and in early infancy Juliette Legler Institute
Integrated Exposure and Effects Assessment (IEEA)
Integrated Exposure and Effects Assessment (IEEA) 11-12 April 2017 Norman workshop Amsterdam Integrated approaches to investigate the effect of progestins in fish and their occurrence in the aquatic environment
Causes and consequences of feminisation of male fish in English rivers
Causes and consequences of feminisation of male fish in English rivers Science Report SC030285/SR The Environment Agency is the leading public body protecting and improving the environment in England and
HOMOSEXYALITY AS A CONSEQUENCE OF EPIGENETICALLY CANALIZED SEXUAL DEVELOPMENT. W.R.Rice, U.Freiberg, S.Gavrilets. December 2012
HOMOSEXYALITY AS A CONSEQUENCE OF EPIGENETICALLY CANALIZED SEXUAL DEVELOPMENT W.R.Rice, U.Freiberg, S.Gavrilets December 2012 Used terms: Epi-marks means changes in chromatin structure that influence the
TBBPA. 31 August 2014 Green Science Policy Madrid, Spain. National Institutes of Health U.S. Department of Health and Human Services
TBBPA Linda S. Birnbaum, Ph.D., D.A.B.T., A.T.S. Director, National Institute of Environmental Health Sciences and National Toxicology Program Principle Investigator, National Cancer Institute 31 August
4. General test considerations
1 1 1 1 1 1 1 1 0 1 0 1. General test considerations.1 Concentration setting Test concentration setting is an important part of the study design. Effective choice of concentrations ensures a successful
Undernutrition during conception and gestation: Increased risk for the next generation
Undernutrition during conception and gestation: Increased risk for the next generation Reinoud J.B.J. Gemke VU University Medical Center Amsterdam www.abcd-study.nl Genetic disorders One gene => one phenotype
Terms. Primary vs. secondary sexual differentiation. Development of gonads vs. physical appearances
SEX DETERMINATION How is the sex of an organism determined? Is this process the same for all organisms? What is the benefit of sexual reproduction anyway? Terms Primary vs. secondary sexual differentiation
EFFECTS OF STRESS ACROSS GENERATIONS: WHY SEX MATTERS
Commentary submitted to Biological Psychiatry EFFECTS OF STRESS ACROSS GENERATIONS: WHY SEX MATTERS Invited commentary on: Saavedra-Rodriguez L, Feig LA (2012): Chronic Social Instability Induces Anxiety
Nature vs nurture: Epigenetics
Nature vs nurture: Epigenetics What is epigenetics? Epigenetic processes control whether a gene is switched on or off (gene expression), without altering the underlying DNA sequence They include modifications
Sex Differentiation. Course Outline. Topic #! Topic lecture! Silverthorn! Membranes (pre-requisite material)!!
Sex Differentiation The goal of these lectures is to discuss how a control system is formed. For this, we will use basic physiology associated with the control of reproduction (from sexual differentiation