Journal of Fishery Sciences of China

Transcription

1 Journal of Fishery Sciences of China Vol. 10 No. 5 Oct ( ; ) : Olympus BH - 2 ( Scylla serrata) 4 : ; ; ; 2 :A ;B A B B A B : ; ; : Q952 :A : (2003) (androgenic gland) ) Wild ( M acrobrachi u m rosenbergii) 1. 2 ( ) [1-4 ] PBS Bouin ( Penaeus chinensis) ( Eriochei r sinen2 6 8 m Ehrlich [5-7 sis) ] - Olympus BH - 2 (Androgenic gland hormone A GH) [8 ] ( Scylla serra ta) [9 ] B : 100 B B 1 10 ( gx SD) 1 B mm g ( : ; : : ( ) ; ( ). : ( ). Tel : E - mail : com :.

2 5 : (- 3) : mm A 5 10 mm B (- 3) 6 8 m (- 1) 60 mm 0. 3 mm : mm mm (- 4) (- 2) m A 9 11 m (- 2 :A 5) ;B A ( 4 5 m 1 2 1) ;B m : mm Developmental / mm stages of Body testis length Spermatogonium stage Spermatocyte stage Table 1 Relation between development of androgenic gland and maturation of testis in Scylla serrata / m Diameter of seminiferous tubule Cross section of / mm androgenic gland B Ratio of mature Ratio of sections B cells Length of androgenic gland / mm Long diameter / mm Short diameter / m Diameter of androgenic glandular cells Spermatid stage Sperm stage / Resting stage / : ; / ; n = 7 ; n = 100 ; n = 10 ; n = 14 Notes : means undetected ; / means unsuitable for statistics as above ; n = 7 ; n = 100 ; n = 10 ; n = 14 : mm A B B 80 % (

3 ) B ( hetoropycnosis) ( Potamon koolooense) ; 1 (- 6) Carpenter Deroos [11 ] 1 (- 7) ; B ; : B ; 1 B A GH ; A GH [10 ] ; 4 : ; ; A GH A B nais) [11 ] - - : ( Pandal us platyceros) 6 [12 6 ] [6 2 ] Taketo2 mi [13 ] ( Procambarus clarkii) 2 Rangneker [14 ] 2 ; : B B B A [15 ] B A A GH 3. 2 Joshi Khanna [16 ] A GH B ( O rconectes A GH [1 ] Nagamine C Knight A W Maggenti A et al. Effect of andro2 genic gland ablation on the male primary and secondary sexual characteristics in the malaysian prawn Macrobrachi um rosen2 bergii (de Man) (Decapoda Palaemonidae) with first evidence of induced ferminization in a nonhermaphroditic decapod [J ]. Comp Endocrinol 1980a 41 : [ 2 ] Nagamine C Knight A W Maggenti A et al. Masculinization of female Macrobrachi um rosenbergii (de Man) (Decapoda Palae2 monidae) by androgenic gland implantation [J ]. docrinol 1980b 41 : Comp En2 [ 3 ] Malecha S R Nevin P A Ha P et al. Sex2reversed freshwater prawns Macrobrachium rosenbergii [J ]. Aqu : [ 4 ] Sagi A Cohen D Milner Y. Effect of androgenic gland ablation on morphotypic differentiation and sexual characteristic of the male freshwater prawns Macrobrachi um rosenbergii [J ]. Comp Endocrinol : [ 5 ]. [J ] (4) :17-21.

4 5 : 379 [ 6 ]. [12 ] Hoffman D L. The development of the androgenic gland of a [J ] (15) : [7 ]. [ 13 ] Taketomi Y. Ultrastructure of the androgenic of the crayfish [J ] (2) : [8 ]. AGH [J ] (2) : [9 ]. trol of reproduction in male crab Scylla serrata ( Forskal) [J ]. [J ] (2) : [10 ]. [J ]. [ 15 ]. [ D ]. : (1) : [ 11 ] Carpenter M B Deroos R. Seasonal morphology and histology of the androgenic gland of the crayfish Orconectes nais [J ]. Comp Endocrinol : proandric shrimp[j ]. Biol Bull : Procambarus clarkii [J ]. Cell Biol Inter Report (2) : [ 14 ] Rangneker P V Madhyastha M N Latey A N. Hormonal con2 J Amin Morphol Physiol (1) : [ 16 ] Joshi P C Khanna S S. Studies on the androgenic gland of the freshwater crab Potamon koolooense ( Rathbum ) [ J ]. Z Mikrosk2anat Forsch Leipzig (4) : Histological study on development of androgenic gland in mud crab Scylla serrata YE Hai2hui 1 2 L I Shao2jing 1 HUAN G Hui2yang 1 WAN G Gui2zhong 1 L IN Qong2wu 1 (1. Department of Oceanography Xiamen University Xiamen China ; 2. College of Life Science Xiamen University Xiamen China) Abstract : The male Scylla serrata individuals were employed with body weight g and body length mm. The observation was conducted under Olympus B H22 microscope. The result s show t hat a pair of an2 drogenic glands attach to t he subterminal portion of t he ejaculatory duct s and t heir develop mental process can be divided into four stages : at Stage the androgenic glands are short and the glandular cells are of small amounts; at Stage the glands distinctively take on a cord - figure ; at Stage the volume of the glands reaches its peak with hyperplasia in some portion ; at Stage the glands no longer grow and degenerate rapid2 ly. There are two types of glandular cells : Type A which has a round nucleus less in heterochromatin and the cytoplasm stained lightly and Type B which has flat nucleus rich in heterochromatin t he cytoplasm stained darkly or vanished. During the development of the androgenic gland the quantity of Type A cells and Type B cells and their ratio in number have some change. After Type A cells perform the secretion function they exist as the form of Type B cells. At the same development stage of andrgenic gland the ratio of Type B cells to Type A cells in number increases significantly which implies that this ratio reflects the secretion activity of the andro2 genic gland. It can be concluded that androgenic gland correlates closely with the mature degree of the testis. Key words : Scylla serrata ; androgenic gland ; development ; histology Corresponding author : L I Shao2jing.

5 : Histological study on development of androgenic gland in mud crab Scylla ser rata Plate Cross section of androgenic gland at Stage Lognitudinal section of androgenic gland at Stage. 31A ( ρ ) B ( ) 330. Type A cells ( ρ ) and Type B cells( ). 41 ( ρ ) 330. Showing the androgenic gland with entwined strand of cells at Stage ( ρ ). 51 A 330. Type A cells develop into follicles at Stage. 61B ( ) 330. Disaggregation of many Type B cells showing the haemocytes( ). 71 ( ρ ) 330. Degeneration of the androgenic gland in the Stage showing sheath( ρ ). AG - Androgenic gland ; ED - Ejaculatory duct