43 (3) : , 1997

Transcription

1 43 (3) : , 1997 A cta Zoologica S i nica 3 1) 2) 3) 4) 3) ( 1), ) ( 2), ) ( 3), ) ( 4),, ) :,,,,,,,,,,,,,,,, (single spike) (multiple spikes),,, 60, ( Crangon septemspi nosa) (Arsenault et al., 1979, 1980 ; Arsenault, 1984) ( Palaemonetes pal udosus ) ( Koehler, 1979) ( Palaemon serrat us) ( Papathanassiu and King, 1984) ( Parapenaeus congi rost ris ) ( medina, 1994 ) ( S icyonia i ngentis ) (Shigekawa and Clark, 1978) ( Enoplometopus occi dentalis) ( Heley, 1986) ( Uca tangei r) ( median and Rodriguez, 1992) Procam barus clarikill ( Moses, 1961) ( Eriochei r si nensis) (, 1988) ( Port unus t rit uberculat us) (, 1995) ( Scylla serrata) (, 1994) , (No1C95030) ;

2 (, 1994), 1,,, (1991) ( 1),,,,, 212,,,,, ( : 2),, ( : 3, 4), ( : 5),, 213, 21311,, ( : 6, 7),,,, ( 8) 21312,,,,,, ( ) ( 9 11), ( 12), 21313, ( 13), ;,, ;, ;,,,,,,

3 3 : 251,, ( : 14),,, ( : 15) 2 3 ( : 16), ( : 16), ( : 14), 3 (1) (, 1995 ;, 1988 ; Arse2 nault, 1984 ; Koehler, 1979 ; Medina et al., 1992 ; Medina, 1994 ; Moses, 1961, Pap2 athanassion et al., 1984 ; Shigekawa and Clark, 1986),, (medina, 1994), ( Pe2 naeus penicillates), 3,,,,,, ( Ucatangeri) (Medina and Rodriguez, 1992) (, 1988) (, 1995),,,,, (kaye et al., 1961) ( ),, Reger (1970), Talbot Summers (1978) ( Panuli nus),, (2) 3,, : 1 ( )

4 252 43,,, (, 1996 ;, 1994),,,,, ( Hinsch, 1973, 1986 ; Pochon2Masson, 1983),, ( Hinsch, 1986 ; Jamieson, 1990, 1991 ; Medine et al., 1992 ; Reger, 1970),,,, (Dendrobrannchiate) ( Medina, 1994) Panuli rus argus P. guttat us ( Talbot and Summers, 1978) (Carideans) (Medina, 1994), (, 1994),,, (1) : 7 11.,, ( ) 35 (6) : (1) : , 1991 : (2) : 1 9., : 1 1 : ,, 1988 : (1) : Arsenault, A. L Changes in the nuclear envelope associated with spermtid differentiation in the shrimp, Cran2 gon septemsinosa. J. Ult rast ruct. Res. 8 : Haley, S. R Ultrastructrur of spermatogenesis in the Hawaiian red lobster, Enoplometopus occidentalis ( Ran2 dall). J. Morph. 190 : Hinsch, G. W Sperm structure of Oxyrhyncha. Can. J. Zool. 51 : Hinsch, G. W A comparison of sperm morphologies, transfer and sperm mass storage between two species of crab, Hinsch, G. Ovalipes ocellat us and L ibinia emarginata. Int. J. Invert. Reprod. Dev. 10 : W Ultrastucture of the sperm and spermatophores of the golden crab Geryon f enneir and a closely relat2 ed species, the red G. quinquedens, from the eastern Gulf of Mexico. J. Crustacean Biol. 8 : Jamieson, B. G. M The ultrastructure of the spermatozoa of Petalomera lateralis ( Gray) (Crustacea, Brachyura, Dromiacea) and its phylogenetic significance. Int. J. Invert. Reprod. Dev. 17 : Jamieson, B. G. M Ultrastructure and phylogeny of crustacean spermatozoa. Mem. Od. M us. 31 :

5 3 : 253 Kaye, G. I. G. D. Pappas, G. Yasuzumi and H. Yamamoto 1961 The distribution and form of the endoplasmic reticulum during spermatogenesis in the crayfish, Cambaroides japonicus. Z. Zellf irsch. 53 : Koehler, L. D. Ult rast ruct. Medina, A. and A. Medina, A. Moses, M. J A unique case of cytodifferentiation : spermiogenesis of the prawn, palaemonetes paludosus. J. Res. 69 : Rodriguez 1992 Spermiogenesis and sperm structure in the crab Uca tangeir (Crustacea, Brachyu2 ra), with special reference to the differentiation. Zoomorphology 111 : Spermiogenesis and sperm structure in the shrimp Parapenaeus longirost ris ( Crustacea : Dendro2 branchiata) : comparative aspects among decapods. Biochem. M at. Biol 119 : Spermiogenesis in the crayfish ( Procambarus clarkii).. Description of stages. J. Biophys. Cytol. 10 : Papathanassiou, E. and P. E. King 1984 Ultrastructural studies on gameto2genesis of the prawn Palaemon serrat us ( Pennant).. Spermiogenesis. Acta. Zool. (Stockh). 65 : Pochon2Masson, J. brates. Vol Arthropoda2Crustacea. In : K. G. Adiyodi et al. eds. Reproductive Biology of Inverte2. Spermatogenesis and sperm function. Bath, Avon : Pitman Press Reger, J. F Studies on the fine structure of spermatids and spermatozoa of the crab Pinnixia sp. J. Morph. 132 : Shigekawa, K. and W. H. J r. Clark 1986 Spermiogenesis in the marine shrimp, Sicyonia ingentis. Dev. Growth Dif f erention 28 : Talbot, P. and R. G. Summers 1978 The structure of sperm from Panulirus, the spiny lobster, with special regard to the acrosome. J. Ult rast ruct. Res. 64 : ( Abstract) UL TRASTRUCTURE OF SPERMATOGENESIS IN THE CRAB SC YLL A S ERRA TA WAN G Yi2Lei 1) 2) 3) ZHAN G Zi2Ping 4) L I Shao2Jing 3) ( 1) L aboratory of Cellular and Molecular Evolution, Kunming Instit ute of Zoology, The Chinese Academy of Sciences, Kunming , China) ( 2) A quacult ure Depart ment, Xiamen Fisheries College, Xiamen , China) ( 3) Oceanography Depart ment, Xiamen U niversity, Xiamen , China) ( 4) Depart ment of Biology and Chemist ry, City U niversity of Hong Kong, Kowloon, Hong Kong) Spermatogenesis in t he crab Scylla serrata was examined using t ransmission elect ron microscopy. The spermatonium has an oval shape, in which t here are dispersed chromatin, mitochondria and endoplasmic reticulum vesicles. The primary spermatocyte contains decon2 densed chromatin, a large amount of endoplasmic reticulum vesicles, special membrane sys2 tem and crystalline lattices. In t he secondary spermatocyte emerges a cavity which is formed by t he aggregation of endoplasmic reticulum vesicles. The early polarized spermatid has a conspicuous proacrosomal vesicle at one pole, a dense apical condensate formed at the top of t he vesicle. A homogeneous nucleus and some cytoplasm containing only t he membrane sys2

6 tem, mitochondria, etc. lie to t he opposite pole. In t he mid2stage spermatid an invagination of the acrosomal vesicle membrane gives rise to the formation of the perforatorium, in which a shapeless tubule originates from the membrane system. The nucleus enlarges and the chro2 matin begins to arrange into fibers. In t he late spermatid t he nucleus differentiates into nu2 merous slender arms, the spherial acrosome further develops into acrosomal tubule and acro2 somal vesicle. The acrosomal vesicle includes an apical cap, a subcap zone, an inner layer and an outer layer. The mature sperm has a top2like shape, the apical cap can be divided into three thin layers (two dense layers and one lucent layer). Outside of the outer layer are 223 lamellar st ruct ures. Microt ubules of t he perforatorium in t he cent ral cannal present a conical appearance. Key words Scylla serrata, Spermatogenesis, Ultrastructure I ( Plate I ) 11 (Spermatogonium) Ch : (chromotin) M : ( mitochondria) N : (nu2 cleus) 21 ( Primary spermatocyte) EV : (endoplasmic reticulum vesicles) MS : (membrane system) 31, ( Primary spermatocyte, showing membrane system and crystalline lattices) CL : (crystalline lattices) 41 (Showing membrane system and crystalling lattices) , (Secondary spermatocyte, showing a cavity which is formed by aggrega2 tion of endoplasmic reticulum vesicles) C : (cavit y) 6 71 ( Early spermatid) CP : (cytoplasm) PV : (proacrosomal vesicle) 81 ( ) ( Early spermatid, showing dense apical condensate (arrow) ) ( Plate ) 91, (Mid2stage spermatid, showing arch structure) AV : (acrosomal vesi2 cle) 101 (A magnified arch structure) (Showing the formation of inner layer of acrosomal vesicle) : (Showing the formation of acrosomal tubule between acrosomal cap and arch struc2 ture) AC : (apical cap) AT : (acrosomal tubule) 131 (Late spermatid) CC : (central cannal) IL : (inner layer) OL : (outer layer) SZ: (subcap zone) 141 (Mature sperm) (, ) (A magnified part of acrosome, showing AC and SZ) ( ) (A magnified part of acrosome, showing lamellar structure and thickened ring) LS : (lamellar structure) TR : (thinkened ring)