11 1 Vol. 11,No. 1, AC TA ZOON U TR IM EN TA SIN ICA J an (,,100094) 1. 1 :

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1 11 1 Vol. 11,No. 1, AC TA ZOON U TR IM EN TA SIN ICA J an (,,100094) 0 3 Avain, 0, 4, 7, 10, 14, 17 21,,, 0 3 : (1) 4 10 ( 19 %),10, 14 21,13 % 14 %; (2) (g/ kg ), 4 7, 7 10, 7 ; (3), 1. 5, 2, 2. 3, 3, 6 ; (4) (g/ kg ) 4 10, ; (5) ( / kg ), 10,7,21 10, 10,,,,,,,,,,,,,,: (1) 0 3 ; (2) :

2 Avain 120,, 3,, : 0,4,7,10,14,17 21,8 : 00 9 : 00, 10, ( ) ( ) ( Table T he com position of deit an d n ut rient level of t he broilers of 0 3 w eek ages Ingredient s ( %) Nut rient level Corn M E (Mcal/ kg) Soybean meal CP ( %) Plant oil Ca ( %) Dicalcium p hosp hate 1. 3 Available P ( %) Limestone L ys ( %) Met Met ( %) L ys Met + Cys ( %) Salt Trace element mix 0. 2 Vitamin mix Total 100 Cu 8mg, Fe 80mg, Zn 75mg, Mn 100mg, Se 0. 3mg, I 0. 35mg, VA 10800IU, VD3 2160IU, V E 4. 6mg, V K3 1. 0mg, VB1 0. 4mg, VB2 5mg, VB12 6ug, 0. 10mg,, 7mg,5mg, ), - 30, :,,W/ V = 1/ 10,W/ V = 1/ 5 4, 30, 20000g4 10, - 30, : :: ; : :, (1994) Folin

3 1 : O Sullivan (1992), 0. 1 % Tris, 40 60min Gertler Nit san (1970), 0. 05M Tris - HCl,p H7. 10,0. 05MCaCl Bernfeld (1955) : 40,p H mol Osullivan (1992),: p H7. 8, 40, 0. 1 mol (Relative daily growt h rate) = 100 % / (Allomet ric growt h) = / U/ g / kg = ,, %, 4 10, 19 %,14,,13 % 14 %, 0 21,,, 4 7, 7 10, 7 (2, 3),, 7, 0 21,,, ,, LiLja (1985) Katanbaf (1988) Sell (1991) Nir (1993),, 0,4 10,,10, 4 10 (3), ,U/ g (5), 0 4,,4,

4 Fi g. 1 Daily rel ati ve grow t h rates of t he m ale broilers of 0-3 w eek ages 2 Fi g. 2 Rel ati ve organ w ei ghts of t he m ale brailers i n dif f erent age periods 3 Fi g. 3 Changes of w ei ghts an d contents i n t he s m all i ntesti ne of t he m ale broilers i n dif f erent age periods Fi g. 4 A llomet ry of t he di gesti ve organs i n t he m ale brailers of 0-3 w eek ags,, ,0 4,, 21 U / kg ( 6), 10, 7, U / mg (9), 10, 10, 21 21,U / g (7),,21 4, U / kg ( 8), 10 U / mg (10), 17, 14

5 1 : Fi g. 5 Changes of acti vity ( U / g of pancrea w ai ght ) pancreatic enz y mes of t he brailers of 0 3 w eek ages Fi g. 6 Changes of rel ati ve acti vity ( U / kg B W ) of pancreatic enz y mes of t he brailes of 0 3 w eek ages Fi g. 7 Changes of acti vity ( U / g of di gest a) of t he enz y mes i n t he digesti ve t ract of t he broilers of 0 3 w eek ages Fi g 8. Changes of rel ati ve acti vity ( U / kg B W ) of enz y mes i n t he digesti ve t ract of t he broilers of 0-3 w eek ages, U / g,es2 cribano (1988) Krogdahl Sell (1989) Sell (1991) Pinchosov No y (1994) Kulka Duksin (1964),,1 5, Marchaim Kulka (1967) 3,,,

6 Fi g. 9 Changes of acti vity ( U / m g p rotei n) of pancreatic enz y mes of t he broilers of 0-3 w eek ages Fi g. 10 Changes of rel ati ve acti vity ( U / m g p rotei n) of pancreatic enz y mes i n t he di gesti ve t ract of t he broilers of 0-3 w eek age Kulda and Duksin (1964) Marchaim Kulka (1967) Ikeno (1991),,,,,,, ( ),,,,, U / kg,,, 10, U / kg,,,, U / g,, (U / kg),,, U / kg,,, 10,, :, Escribano F, Sell L Develop ment of lipase activity in yolk membrane and pancreas of young t urkeys. Poult. S ci.,67 : Ikeno T, Ikeno K Amylase activity increase in t he yolk of fertilized eggs during incubation in chick -

7 1 : ens. Poult. S ci.,70 : Krogdhal A,Sell J L Influnce of age on lipase,amylase and protease activities in pancreatic tissue and intestinal content s of young t urkeys. Poult. S ci.,68 : Kulka R G,Duksin D Patterns growt h and - amylase activity in t he developing chick pancreas. B iochi m. B iophys. A ct a,91 : L aws B M,Moore J H Some observation on t he pancreatic amylase and intestinal maltase of t he chick. J B ilchem. physiol.,41 : Marchaim V, Kulka R G The non - parallel increase of amylase,chymot ryp sinogen and p rocar - boxypeptidase in t he developing chick pancreas. B iochi m. B iophys. A ct a,146 : Nir I Nit san Z,Mahagna M Comparative growt h and develop ment of t he digestive organs and of some enzymes in broiler and egg type chicks after hatching. B r. Poult. S ci., 34 : Nit san Z A,Dunnington E A,Siegel P B. 1991a. Organ growt h and digestive enzyme levels to fifteen days of age in lines of chickens differing in body weight. Poult. S ci.,70 : Nit san Z Ben - Avraham G,Zoref Z,Nir I. 1991b. Growt h and develop ment of digestion organ and some en - zymes in broiler chicks after hatching. B r. Poult. S ci.,32 : Noy Y,Sklan D Digestion and absorption in t he young chicks. Poult. S ci.,74 : O Sullivan N P,Dunnington E A,Larsen A S,Siegel P B. 1992a. Correlated responses in lines of chickens divergently selected for fifty - six - day body weight. 3. digestive enzymes. Poult. S ci.,71 : STUD IES O N D EVELOPMENT OF D IGESTIVE ENZYMES IN BROIL ER CHICKS A n Yongyi Zhou Y u pi ng Guo Y u m i ng Di ng J i aoli Zhu Y uqi ng ( College of A ni m al S cience an d Technology, Chi na A gricult u ral U ni versity, Beiji ng, ) ABSTRACT Avain male broiler chicks of 0 3 weeks were used as experimental animals. On day 0, 4, 7, 10, 14, 17and 21, ten chicks were weighed and killed, respectilely, t hen t he liver, pan2 creas,small intestine,gizzard,p rovent riculus,residual yolk and chyme in t he small intestine were weighed, and t he activities of amylase, lipase, t ryp sin and chymot ryp sin were mea2 sured. The result s were as follows : (1) Relative daily growt h rate peaked at 4 10 d of age (19 % gain/ d) and t hen decreased gradually ; at d of age maintained in t he range of 13 % 14 % gain/ d. (2) Relative weight (g/ kg body weight) of various organs peaked at dif2 ferent days. i. e. at 4 7 d for t he p rovent riculus and gizzard, at 7 10 d for t he liver and pancreas, 7 d fo r t he small intestine. ( 3) Maximal allo met ric growt hs, of t he gizzard, p rovent riculus, liver, small intestine and pancreas were 1. 5, 2, 2. 3, 3 and 6 - fold greater

8 24 11 t han t hat of t he body. (4) Relative weight (g/ kg body weight) of chyme in t he small intestine peaked at 4 10 d of age and t hen decreased. (5) Activities (unit s/ kg body weight) of t he digestive enzyme measured in t he pancreas and intestinal content s increased wit h age. In t he pancreas maximal values were attained on day 10 for amylase on day, 7 for t ryp sin, on day 21 for chymot ryp sin and on 10 for lipase. In t he small intestine maxima were attained on day 10 for amylase,t ryp sin,chymot ryp sin and lipase. Key word : Broiler, Amylase, Tryp si n, Chymot ryp sin,lipase,,,,,, , : : : : : : 3. : 1. : (1) ; (2) ; (3) ; (4) ; (5) ; (6) ; (7) ; 2. : (, ) 3. : (1) 3000, (,600 ) (2),, (3) 4. : : : : ( ) 400, ( 60 ), :, , : (1) : : : : : (2) : : 306 : : :010018