Quality Led and Customer Driven
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- Samuel Webb
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1 Quality Led and Customer Driven
2 INTRODUCTION Serana Europe GmbH is a leading manufacturer and supplier of cell culture products. Our product range includes animal & human sera, classical & serum free media as well as liquid & powdered media, reagents, supplements, buffer solutions for cell culture and diagnostic applications. Serana s products are used in all areas where cell culture is performed. This includes the biopharmaceutical industry for the production of vaccines, therapeutic proteins and diagnostics. In addition, we are a major supplier to the Academic R&D (universities, hospitals & clinics), private research institutes and small biotech companies. We have manufacturing facilities in Germany and Australia which makes us an ideal partner for both industrial and research organizations. Our website enables you to find all available products, product information, various protocols, certificates, and material safety data sheets. Serana Quality Led and Customer Driven 1
3 TABLE OF CONTENTS Sera 3 FBS 4 FBS Gamma Irradiated 4 FBS Heat Inactivated 4 FBS Ultra Low IgG 4 FBS Dialysed 4 Newborn Calf Serum 5 Calf Serum 5 Horse Serum 5 Goat Serum 5 Chicken Serum 5 Porcine Serum 5 Human Serum, off the clot 6 Human Serum, converted 6 Media 7 DMEM, Low Glucose 8 DMEM, High Glucose 9 DMEM / Hams s F Medium RPMI Reagents 13 Bovine Serum Albumin 13 Secoll - Lymphocyte Separation Solution 14 Antibiotics Trypsin L-Glutamine G Buffers 16 DPBS 16 HBSS 16 Sodium Bicarbonate Solution 16 Sodium Pyruvate Solution 16 HEPES Solution 16 Purified Water 16 Serana Quality Led and Customer Driven 2
4 SERA INTRODUCTION Sera Serum is the blood component remaining after coagulation and removal of the cellular components usually through centrifugation. Serum is a complex mixture of proteins including growth factors, amino acids and hormones. This complicated mixture makes serum to be one of the most important supplements to support cells to grow and proliferate in vitro cell culture. FBS Quality and Experience Serana's foetal bovine serum (FBS) is sourced from only the highest quality raw material sources in Australia, United States, and South America. We have extensive industry experience manufacturing to the highest of standards. Serana maintains an in-house quality control department in order to verify the products adhere to the strictest of quality specifications. Speak with a Serana representative today about your foetal bovine serum requirements in order to receive your free samples. Production and Traceability Fetal Bovine Serum passes through a series of filters terminating with triple 0.1 µm pore size pharmaceutical grade sterile filters. The product is dispensed in a Class 100 laminar flow workstation via a closed system and then immediately frozen. From collection of raw sera through to the final sterile filtered product, Serana manages and documents all processes involved. This certifies traceability to the end product. Batch records including Certificates of Origin, Certificates of Analysis and other batch release documents are maintained and regularly audited. Treatments Serana s FBS products are available with the following treatments: gamma irradiated, charcoal stripped, heat inactivated, dialysed, and Ultra Low IgG. Storage, Shipping and Logisitics All sera is stored in - 15 C controlled freezer facilities. Serana ships serum products in polystyrene boxes containing sufficient dry ice to last throughout its journey. Test Samples, Reservations and Orders Many customers prefer to test serum products with their own cell lines to follow their in-house requirements. Serana s distributors will provide samples from different batches for customer testing and will hold the requested numbers of bottles from each batch on reservation until sample tests are completed. Normally, reservations are held for 4 weeks. Please advise your distributor if longer reservations are required. Precaution All serum products are for in vitro laboratory use or further manufacturing only. Serana Quality Led and Customer Driven 3
5 Foetal Bovine Serum FOETAL BOVINE SERUM Applications Growth promoting additive to cell culture media. Stabilizer in diagnostic assays. Supports most cell lines. for viruses and microorganisms. Product Specifications ph Osmolality Total Protein Albumin IgG Haemoglobin Mycoplasma Virus/Antibody Tests Sterility Long Term Storage mosmol/kg 10 EU/ml g/dl g/dl 250 µg/ml 20 mg/dl Not detected PI-3, BVD-AB, BVD-V, BHV-1-15 C Product Product Description Origin Volume S-FBS-SA-015 Foetal Bovine Serum Triple 0.1 µm Sterile Filtered South American 500ml S-FBS-SA-011 Foetal Bovine Serum Triple 0.1 µm Sterile Filtered South American 100ml S-FBS-US-015 Foetal Bovine Serum Triple 0.1 µm Sterile Filtered United States 500ml S-FBS-US-011 Foetal Bovine Serum Triple 0.1 µm Sterile Filtered United States 100ml S-FBS-AU-015 Foetal Bovine Serum Triple 0.1 µm Sterile Filtered Australia 500ml S-FBS-AU-011 Foetal Bovine Serum Triple 0.1 µm Sterile Filtered Australia 100ml for Special Treatments Last 3 digits of product code Treatment Description 025 and 021 Heat Inactivated 500ml and 100ml 035 and 031 Gamma Irradiated 500ml and 100ml 045 and 041 Charcoal Stripped 500ml and 100ml 055 and 051 Ultra Low IgG 500ml and 100ml 065 and 061 Dialysed 500ml and 100ml Serana Quality Led and Customer Driven 4
6 Bovine Serum BOVINE AND OTHER ANIMAL SERUM Calf Serum and Newborn Calf Serum Calf Serum contain higher concentrations of IgG than Fetal Bovine Serum and has an increased protein content. Newborn and Calf serum can be cost effective alternatives to Fetal Bovine Serum. Product Product Description Origin Age of Animal Volume S-NC-US-015 Newborn Calf Serum 0.2 µm Sterile Filtered United States < 12 days 500ml S-NC-NZ-015 Newborn Calf Serum 0.2 µm Sterile Filtered New Zealand < 12 days 500ml S-NC-EU-015 Newborn Calf Serum 0.2 µm Sterile Filtered EU Eligible < 12 days 500ml S-NC-AU-015 Newborn Calf Serum 0.2 µm Sterile Filtered Australia < 12 days 500ml S-CS-US-015 Calf Serum 0.2 µm Sterile Filtered United States < 12 months 500ml S-CS-NZ-015 Calf Serum 0.2 µm Sterile Filtered New Zealand < 12 months 500ml S-CS-EU-015 Calf Serum 0.2 µm Sterile Filtered EU Eligible < 12 months 500ml S-CS-AU-015 Calf Serum 0.2 µm Sterile Filtered Australia < 12 months 500ml Other Animal Serum Product Product Description Origin Volume S-HS-US-015 Horse Serum 0.2 µm Sterile Filtered United States 500ml S-HS-EU-015 Horse Serum 0.2 µm Sterile Filtered EU Eligible 500ml S-CH-US-015 Chicken Serum 0.2 µm Sterile Filtered United States 500ml S-CH-EU-015 Chicken Serum 0.2 µm Sterile Filtered EU Eligible 500ml S-GS-US-015 Goat Serum 0.2 µm Sterile Filtered United States 500ml S-GS-EU-015 Goat Serum 0.2 µm Sterile Filtered EU Eligible 500ml S-PS-US-015 Porcine Serum 0.2 µm Sterile Filtered United States 500ml S-PS-EU-015 Porcine Serum 0.2 µm Sterile Filtered EU Eligible 500ml Serana Quality Led and Customer Driven 5
7 Human Serum HUMAN SERUM Applications Additive for sensitive cell cultures Suitable for most human cells Recommended for lymphocytes and human macrophages Clinical chemistry In vitro diagnostic use Product Specifications Mycoplasma Virus Tests Cell Culture Sterility Long Term Storage Not detected HBAg, HCV, Anti HIV 1/2-18 C Converted and Off the Clot Off the clot is obtained from whole blood which has not been treated with anticoagulants. It goes through the natural blood clotting process and then it is centrifuged. This is in contrast to converted human serum, which has had an anticoagulant added prior to centrifugation, and then is converted to serum from plasma. Precaution All Human Sera has been internally and externally tested, however all human products should be treated as potentially infectious agents and in accordance with universal handling precautions. All human sera should only be used for in vitro diagnostic and research purposes, and not for therapeutic use. Product Product Description Origin Volume S-HU-US-015 Human Serum Off the clot United States 500ml S-HU-US-011 Human Serum Off the clot United States 100ml S-HU-US-025 Human Serum Off the clot, Type AB United States 500ml S-HU-US-021 Human Serum Off the clot, Type AB United States 100ml S-HU-US-035 Human Serum Converted United States 500ml S-HU-US-031 Human Serum Converted United States 100ml S-HU-US-045 Human Serum Converted, Type AB United States 500ml S-HU-US-041 Human Serum Converted, Type AB United States 100ml Serana Quality Led and Customer Driven 6
8 MEDIA INTRODUCTION Manufacuring of Liquid Media Serana cell culture media is manufactured under stringent standard operating procedures. A variety of tests are performed including endotoxin, ph value, and osmolality on each lot of 0.2 µm sterile filtered media. Only batches that pass Serana s rigorous quality control procedures are released for sale. Detailed certificates of analysis are prepared and made available for each lot produced. Packaging All sterile filtered liquid media are filled into PET bottles of 100ml and 500ml in volume. Special filling sizes are available on customer request. Storage and Shipping Unless otherwise stated on the product label, Serana s cell culture media should be stored at 2 C to 8 C for long term storage. However, short-term storage at room temperature is possible. Stable Glutamine L-Alanyl-L-Glutamine contains a stabilized dipeptide form of L- Glutamine and can be metabolized by many cells in the same manner as L-Glutamine. Serana uses this stable form of L- Glutamine in many of its cell culture media. Customized Manufacturing In addition to Serana s standard QC control procedures, many additional tests can be carried out upon our clients request. Also, should you require a component or a test that is not specifically listed, please speak to your customer representative to see if your custom request can be accomodated into our manufacuring and QC processes. Precaution All our media is intended for in vitro laboratory use or further manufacturing only. L-Glutamine L-Glutamine is an essential amino acid which is required for cell culture, however, it can degrade in storage or during incubation. Therefore Serana recommends the addition of fresh L-Glutamine to the cell culture medium at the time of use. Serana supplies frozen L-Glutamine which can be used for this reason. Serana Quality Led and Customer Driven 7
9 DMEM, Low Glucose CLASSICAL MEDIA Specifications Liquid Product Specifications Sterility ph Osmolality Mycoplasma Cell Culture Storage Clear, Red Solution mm/kg +2 C to +8 C Note: NaHCO3 is not included in the powder media formulation. Powder Product Specifications Solubililty Moisture Content Particle Size (100 mesh) ph without NaHCO3 ph with 3700 mg/l NaHCO3 Osmolality without NaHCO3 Osmolality with 3700 mg/l NaHCO3 Peach Powder Pass 2 % 90 % Record Record Record mm/kg Record mm/kg Formulation Components mg/l Components mg/l Calcium Chloride, Anhydrous (CaCl2) Choline Chloride D-Calcium Pantothenate (C18H32CaN2O10) D-Glucose, Anhydrous (C6H12O6) Ferric Nitrate, Nonahydrate (Fe(NO3)3 9H2O) Folic Acid (C19H19N7O6) Glycine (C2H5NO2) HEPES L-Arginine, Hydrochloride (C6H14N4O2 HCl) L-Cystine, Dihydrochloride (C6H12N2O4S2 2HCl) L-Glutamine (C5H10N2O3) L-Histidine, Hydrochloride, Monohydrate (C6H9N3O2 HCl H2O) L-Isoleucine (C6H13NO2) L-Leucine (C6H13NO2) L-Lysine, Hydrochloride (C6H14N2O2 HCl) L-Methionine (C5H11NO2S) L-Phenylalanine (C9H11NO2) L-Serine (C3H7NO3) L-Threonine (C4H9NO3) L-Tryptophan (C11H12N2O2) L-Tyrosine, Disodium, Dihydrate (C9H9NO3Na2 2H2O) L-Valine (C5H11NO2) Magnesium Sulfate, Anhydrous (MgSO4) Myo-Inositol (C6H12O6) Niacinamide (C6H6N2O) Phenol Red, Sodium Salt (C19H13NaO5S) Potassium Chloride (KCl) Pyridoxine, Hydrochloride (C8H11NO3 HCl) Pyruvic Acid, Sodium Salt (C3H3NaO3) Riboflavin (C17H20N4O6) Sodium Bicarbonate (NaHCO3) Sodium Chloride (NaCl) Sodium Phosphate, Monobasic, Monohydrate (NaH2 PO4 H2O) Thiamine, Hydrochloride (C12H17ClN4OS HCl) Liquid Product Powder Product Description Liquid Volume Powder Makes MCL ML MCP L With low-glucose, L-glutamine and sodium pyruvate 500ml 50L MCL ML MCP L With low-glucose, L-glutamine and sodium pyruvate. Without phenol red 500ml 50L MCL ML MCP L With low-glucose, 25mM HEPES, L-glutamine and sodium pyruvate 500ml 50L MCL ML MCP L With low-glucose and sodium pyruvate. Without L-Glutamine and phenol red 500ml 50L MCL ML MCP L With low-glucose and sodium pyruvate. Without L-Glutamine 500ml 50L MCL ML MCP L With low glucose and sodium pyruvate. Without L-Glutamine, L-Arginine, L-Lysine, L- Methionine and Glycine 500ml 50L Serana Quality Led and Customer Driven 8
10 DMEM, High Glucose CLASSICAL MEDIA Specifications Liquid Product Specifications Sterility ph Osmolality Mycoplasma Cell Culture Storage Clear, Red Solution mm/kg +2 C to +8 C Note: NaHCO3 is not included in the powder media formulation. Powder Product Specifications Solubililty Moisture Content Particle Size (100 mesh) ph without NaHCO3 ph with 3700 mg/l NaHCO3 Osmolality without NaHCO3 Osmolality with 3700 mg/l NaHCO3 Off White to Light Orange Powder Pass 2 % 90 % Record Record Record mm/kg Record mm/kg Formulation Components mg/l Components mg/l Calcium Chloride, Anhydrous (CaCl2) Choline Chloride D-Calcium Pantothenate (C18H32CaN2O10) D-Glucose, Anhydrous (C6H12O6) Ferric Nitrate, Nonahydrate (Fe(NO3)3 9H2O) Folic Acid (C19H19N7O6) Glycine (C2H5NO2) HEPES L-Arginine, Hydrochloride (C6H14N4O2 HCl) L-Cystine, Dihydrochloride (C6H12N2O4S2 2HCl) L-Glutamine (C5H10N2O3) L-Histidine, Hydrochloride, Monohydrate (C6H9N3O2 HCl H2O) L-Isoleucine (C6H13NO2) L-Leucine (C6H13NO2) L-Lysine, Hydrochloride (C6H14N2O2 HCl) L-Methionine (C5H11NO2S) L-Phenylalanine (C9H11NO2) L-Serine (C3H7NO3) L-Threonine (C4H9NO3) L-Tryptophan (C11H12N2O2) L-Tyrosine, Disodium, Dihydrate (C9H9NO3Na2 2H2O) L-Valine (C5H11NO2) Magnesium Sulfate, Anhydrous (MgSO4) Myo-Inositol (C6H12O6) Niacinamide (C6H6N2O) Phenol Red, Sodium Salt (C19H13NaO5S) Potassium Chloride (KCl) Pyridoxine, Hydrochloride (C8H11NO3 HCl) Pyruvic Acid, Sodium Salt (C3H3NaO3) Riboflavin (C17H20N4O6) Sodium Bicarbonate (NaHCO3) Sodium Chloride (NaCl) Sodium Phosphate, Monobasic, Monohydrate (NaH2 PO4 H2O) Thiamine, Hydrochloride (C12H17ClN4OS HCl) Liquid Product Powder Product Description Liquid Volume Powder Makes MCL ML MCP L With high-glucose, L-glutamine, and sodium pyruvate. 500ml 50L MCL ML MCP L With high-glucose, sodium pyruvate and L-glutamine. Without phenol red. 500ml 50L MCL ML MCP L With high-glucose, and L-glutamine. Without sodium pyruvate. 500ml 50L MCL ML MCP L With high-glucose. Without L-glutamine and sodium pyruvate. 500ml 50L MCL ML MCP L With high-glucose, L-glutamine, and 25 mm HEPES. Without sodium pyruvate. 500ml 50L MCL ML MCP L With high-glucose, L-glutamine, and 25mM HEPES. Without sodium pyruvate and phenol 500ml 50L red. MCL ML MCP L With high-glucose and sodium pyruvate. Without L-glutamine. 500ml 50L MCL ML MCP L With high-glucose, and L-glutamine. Without sodium pyruvate, and myo-inositol. 500ml 50L MCL ML MCP L With high-glucose, L-glutamine and 1500mg/L sodium bicarbonate. Without L-arginine 500ml 50L and L-Lysine. MCL ML MCP L With high-glucose and L-glutamine. Without sodium phosphate and sodium pyruvate. 500ml 50L MCL ML MCP L With high-glucose, L-glutamine, sodium pyruvate, and HEPES buffer. 500ml 50L MCL ML MCP L With high-glucose. Without L-glutamine, sodium pyruvate, and phenol red. 500ml 50L MCL ML MCP L With high-glucose and 25 mm HEPES. Without L-glutamine. 500ml 50L Serana Quality Led and Customer Driven 9
11 DMEM / Ham s F-12 Specifications Liquid Product Specifications Sterility ph Osmolality Mycoplasma Cell Culture Storage Clear, Red Solution mm/kg +2 C to +8 C Note: NaHCO3 is not included in the powder media formulation. Powder Product Specifications Solubililty Moisture Content Particle Size (100 mesh) ph without NaHCO3 ph with 3700 mg/l NaHCO3 Osmolality without NaHCO3 Osmolality with 3700 mg/l NaHCO3 Red to Orange Powder Pass 2 % 90 % Record Record mm/kg mm/kg CLASSICAL MEDIA Formulation Components mg/l Components mg/l Biotin (C10H16N2O3S) L-Serine (C3H7NO3) Calcium Chloride, Anhydrous (CaCl2) L-Threonine (C4H9NO3) Choline Chloride L-Tryptophan (C11H12N2O2) Cupric Sulfate, Pentahydrate (CuSO4. 5H2O) L-Tyrosine, Disodium, Dihydrate (C9H9NO3Na2. 2H2O) D-Calcium Pantothenate (C18H32CaN2O10) L-Valine (C5H11NO2) D-Glucose, Anhydrous (C6H12O6) Linoleic Acid (C18H32O2) Ferric Nitrate, Nonahydrate (Fe(NO3)3. 9H2O) Lipoic Acid (DL-Thiotic Acid) (C8H14O2S2) Ferrous Sulfate, Heptahydrate (FeSO4. 7H2O) Magnesium Chloride, Anhydrous (MgCl2) Folic Acid (C19H19N7O6) Magnesium Sulfate, Anhydrous (MgSO4) Glycine (C2H5NO2) Myo-Inositol (C6H12O6) Hypoxanthine, Sodium (C5H3N4O.Na) Niacinamide (C6H6N2O) L-Alanine (C3H7NO2) Phenol Red, Sodium Salt (C19H13NaO5S) L-Arginine, Hydrochloride (C6H14N4O2. HCl) Potassium Chloride (KCl) L-Asparagine, Monohydrate (C4H8N2O3. H2O) Putrescine, Dihydrochloride (C4H12N2. 2HCl) L-Aspartic Acid (C4H7NO4) Pyridoxine, Hydrochloride (C8H11NO3. HCl) L-Cysteine, Hydrochloride, Monohydrate (C3H7NO2. HCl. H2O) Pyruvic Acid, Sodium Salt (C3H3NaO3) L-Cystine, Dihydrochloride (C6H12N2O4S2. 2HCl) Riboflavin (C17H20N4O6) L-Glutamic Acid (C5H9NO4) Sodium Bicarbonate (NaHCO3) L-Glutamine (C5H10N2O3) Sodium Chloride (NaCl) L-Histidine, Hydrochloride, Monohydrate (C6H9N3O2. HCl. H2O) Sodium Phosphate Dibasic, Anhydrous (Na2HPO4) L-Isoleucine (C6H13NO2) Sodium Phosphate, Monobasic, Monohydrate (NaH2 PO4. H2O) L-Leucine (C6H13NO2) Thiamine, Hydrochloride (C12H17ClN4OS. HCl) L-Lysine, Hydrochloride (C6H14N2O2. HCl) Thymidine (C10H14N2O5) L-Methionine (C5H11NO2S) Vitamin B12 (C63H88CoN14O14P) L-Phenylalanine (C9H11NO2) Zinc Sulfate, Heptahydrate (ZnSO4. 7H2O) L-Proline (C5H9NO2) Liquid Product Powder Product Description Liquid Volume MCL ML MCP L With stabilized L-glutamine. 500ml 50L MCL ML MCP L With L-glutamine and 15mM HEPES. Without choline chloride and phenol red. 500ml 50L MCL ML MCP L With L-glutamine and 15 mm HEPES. 500ml 50L MCL ML MCP L With L-glutamine and 15mM HEPES. Without phenol red. 500ml 50L MCL ML MCP L With L-glutamine and sodium pyruvate. Without HEPES. 500ml 50L MCL ML MCP L With sodium pyruvate. Without L-glutamine and HEPES. 500ml 50L MCL ML MCP L With L-glutamine. Without L-arginine and L-lysine. 500ml 50L MCL ML MCP L With stabilized L-glutamine. Without L-arginine, L-lysine, and L-methionine 500ml 50L Powder Makes Serana Quality Led and Customer Driven 10
12 Medium 199 Specifications Liquid Product Specifications Sterility ph Osmolality Mycoplasma Cell Culture Storage Clear, Red Solution mm/kg +2 C to +8 C Note: NaHCO3 is not included in the powder media formulation. Powder Product Specifications Solubililty Moisture Content Particle Size Distribution ph without NaHCO3 ph with 3700 mg/l NaHCO3 Osmolality without NaHCO3 Osmolality with 3700 mg/l NaHCO3 Red to Orange Powder Pass 2 % complies Record Record mm/kg mm/kg CLASSICAL MEDIA Formulation Components mg/l Components mg/l (+/-) a-tocopherol Phosphate, Disodium Salt (C29H49Na2O5P) L-Hydroxyproline (C5H9NO3) Deoxy-D-Ribose (C5H10O4) L-Isoleucine (C6H13NO2) Adenine Sulfate, Dihydrate (C10H12N10O4S) L-Leucine (C6H13NO2) Adenosine 5'-Monophosphate, Monohydrate (C10H14N5O7P. H2O) L-Lysine, Hydrochloride (C6H14N2O2. HCl) Adenosine-5'-Triphosphate, Anhydrous (C10H14N5Na2O13P3) L-Methionine (C5H11NO2S) Biotin (C10H16N2O3S) L-Phenylalanine (C9H11NO2) Calciferol (C28H44O) L-Proline (C5H9NO2) Calcium Chloride, Anhydrous (CaCl2) L-Serine (C3H7NO3) Cholesterol L-Threonine (C4H9NO3) Choline Chloride L-Tryptophan (C11H12N2O2) D-Calcium Pantothenate (C18H32CaN2O10) L-Tyrosine, Disodium, Dihydrate (C9H9NO3Na2.2H2O) D-Glucose, Anhydrous (C6H12O6) L-Valine (C5H11NO2) D-Ribose (C5H10O5) Magnesium Sulfate, Anhydrous (MgSO4) Ferric Nitrate, Nonahydrate (Fe(NO3)3.9H2O) Menadione (C11H8O2) Folic Acid (C19H19N7O6) Myo-Inositol (C6H12O6) Glutathione, Reduced (C10H17N3O6S) Niacinamide (C6H6N2O) Glycine (C2H5NO2) Nicotinic Acid (C6H5NO2) Guanine HCl (C5H5N5O. HCl) Para-Aminobenzoic Acid (C7H7NO2) Hypoxanthine, Sodium (C5H3N4O.Na) Phenol Red, Sodium Salt (C19H13NaO5S) L-Alanine (C3H7NO2) Potassium Chloride (KCl) L-Arginine, Hydrochloride (C6H14N4O2. HCl) Pyridoxine, Hydrochloride (C8H11NO3. HCl) L-Ascorbic Acid (C6H8O6) Riboflavin (C17H20N4O6) L-Aspartic Acid (C4H7NO4) Sodium Acetate, Anhydrous (C2H3O2Na) L-Cysteine, Hydrochloride, Monohydrate (C3H7NO2. HCl. H2O) Sodium Bicarbonate (NaHCO3) L-Cystine, Dihydrochloride (C6H12N2O4S2. 2HCl) Sodium Chloride (NaCl) L-Glutamic Acid (C5H9NO4) Sodium Phosphate, Monobasic, Monohydrate (NaH2PO4. H2O) L-Glutamine (C5H10N2O3) Thiamine, Hydrochloride (C12H17ClN4OS. HCl) L-Histidine, Hydrochloride, Monohydrate (C6H9N3O2. HCl. H2O) Liquid Product Powder Product Description Liquid Volume MCL ML MCP L With Earle's salts and L-glutamine. Without phenol red. 500ml 50L MCL ML MCP L With Earle's salts and L-glutamine. 500ml 50L MCL ML MCP L With Earle's salts. Without L-glutamine. 500ml 50L Powder Makes Serana Quality Led and Customer Driven 11
13 RPMI 1640 Specifications Liquid Product Specifications Sterility ph Osmolality Mycoplasma Cell Culture Storage Clear, Red Solution mm/kg +2 C to +8 C Note: NaHCO3 is not included in the powder media formulation. Powder Product Specifications Solubililty Moisture Content Particle Size Distribution ph without NaHCO3 ph with 3700 mg/l NaHCO3 Osmolality without NaHCO3 Osmolality with 3700 mg/l NaHCO3 Red to Orange Powder Pass 2 % complies Record Record mm/kg mm/kg CLASSICAL MEDIA Formulation Components mg/l Components mg/l Biotin (C10H16N2O3S) L-Phenylalanine (C9H11NO2) Calcium Nitrate, Tetrahydrate (Ca(NO3)2. 4H2O) L-Proline (C5H9NO2) Choline Chloride L-Serine (C3H7NO3) D-Calcium Pantothenate (C18H32CaN2O10) L-Threonine (C4H9NO3) D-Glucose, Anhydrous (C6H12O6) L-Tryptophan (C11H12N2O2) Folic Acid (C19H19N7O6) L-Tyrosine, Disodium, Dihydrate (C9H9NO3Na2. 2H2O) Glutathione, Reduced (C10H17N3O6S) L-Valine (C5H11NO2) Glycine (C2H5NO2) Magnesium Sulfate, Anhydrous (MgSO4) L-Arginine (C6H14N4O2) Myo-Inositol (C6H12O6) L-Asparagine, Anhydrous (C4H8N2O3) Niacinamide (C6H6N2O) L-Aspartic Acid (C4H7NO4) Para-Aminobenzoic Acid (C7H7NO2) L-Cystine, Dihydrochloride (C6H12N2O4S2. 2HCl) Phenol Red, Sodium Salt (C19H13NaO5S) L-Glutamic Acid (C5H9NO4) Potassium Chloride (KCl) L-Glutamine (C5H10N2O3) Pyridoxine, Hydrochloride (C8H11NO3. HCl) L-Histidine, Anhydrous (C6H9N3O2) Riboflavin (C17H20N4O6) L-Hydroxyproline (C5H9NO3) Sodium Bicarbonate (NaHCO3) L-Isoleucine (C6H13NO2) Sodium Chloride (NaCl) L-Leucine (C6H13NO2) Sodium Phosphate Dibasic, Anhydrous (Na2HPO4) L-Lysine, Hydrochloride (C6H14N2O2. HCl) Thiamine, Hydrochloride (C12H17ClN4OS. HCl) L-Methionine (C5H11NO2S) Vitamin B12 (C63H88CoN14O14P) Liquid Product Powder Product Description Liquid Volume MCL ML MCP L With L-glutamine. Without phenol red. 500ml 50L MCL ML MCP L With L-glutamine. Without L-methionine. 500ml 50L MCL ML MCP L With L-glutamine. 500ml 50L MCL ML MCP L With L-glutamine and 25 mm HEPES buffer. 500ml 50L MCL ML MCP L Without L-glutamine. 500ml 50L MCL ML MCP L Without amino acids. 500ml 50L MCL ML MCP L With stabilized L-glutamine. 500ml 50L MCL ML MCP L Without L-glutamine and phenol red. 500ml 50L MCL ML MCP L With 25 mm HEPES buffer. Without L-glutamine. 500ml 50L Powder Makes Serana Quality Led and Customer Driven 12
14 Bovine Serum Albumin BOVINE SERUM ALBUMIN Manufacturing Overview Using the heat shock method plus purification by extensive diafiltration at a low ph gives a more purified product (albumin fraction) than the Cohn process. This process is taking place in a closed system. Thawed Bovine Plasma Heating Process Cooling, dilution and ph adjustment Centrifugation Precipitation Waste Filtration Water Ultra and Diafiltration Permeate Waste Sodium Hydroxide ph adjustment Sterile Filtration Freezing process Freeze drying BSA Powder Storage and Stability Bovine Serum Albumin should be stored in tight and original containers at a temperature between 2-8 C. BSA is stable at room temperatures for several months and can be shipped at ambient temperatures. However, for long-term storage, the product must be kept under cool and dry conditions, as moisture can affect the shelf life of the product. It is stable for at least 3 years from the date of production. Product Description Product Specifications Protein Purity Moisture IgG Protease RBP-001-1KG BSA New Zealand Origin Lyophilized coarse powder 96.0% 98.0% 5.0% 0.005% 10 EU/mg 0.05% 1 kg RBP-002-1KG BSA New Zealand Origin Lyophilized fine powder 96.0% 98.0% 5.0% 0.005% 10 EU/mg 0.05% 1 kg RBP-003-1KG BSA New Zealand Origin Protease free 96.0% 98.0% 5.0% 0.005% Not Detected 10 EU/mg 0.05% 1 kg RBP-004-1KG BSA New Zealand Origin Stripped 98.0% 99.0% 5.0% 0.005% Not Detected 10 EU/mg 0.05% 1 kg RBP-005-1KG BSA New Zealand Origin ph % 98.0% 5.0% 0.005% 10 EU/mg 0.05% 1 kg RBP-006-1KG BSA New Zealand Origin Low 96.0% 98.0% 5.0% 0.005% 5 EU/mg 0.05% 1 kg RBP-007-1KG BSA New Zealand Origin Protease, IgG and Enzyme free 98.0% 99.0% 5.0% 0.001% Not Detected 10 EU/mg 0.05% 1 kg RBP-008-1KG BSA New Zealand Origin Protease, IgG, Enzyme and free 98.0% 99.0% 5.0% 0.001% Not Detected < 1 EU/mg 0.05% 1 kg RBP-009-1KG BSA New Zealand Origin Totally Free of Fatty Acid (TFFA) 98.0% 99.0% 5.0% 0.005% Not Detected 10 EU/mg 0.01% 1 kg Fatty Acid Ask for Bulk Pricing Serana Quality Led and Customer Driven 13
15 Secoll Separation Solution LYMPHOCYTE SEPARATION SOLUTION - SECOLL Secoll separation solution contains a polymer with a molecular weight of approximately 400 kda. Densities of up to 1.1 g/ml can be made to order using this hydrophilic polymer. Intended Use and Storage Secoll separation solution is used for isolation of cells or cellular particles during density gradient centrifugation. If the intended use of the separated cells involves subsequent culturing, this sterile separating solution is ideal. A Certificate of Analysis is supplied with all Secoll orders. Shelf life is 3 years and store at 15 C to 25 C in darkness. Specifications Visual: ph: Osmolality: Density: : Sterility: Clear colorless liquid free of particulate matter 6.5 to to 310 mosm/kg C < 4 EU/ml Sterile Secoll Usage Diagram Secoll Density Chart Product Description Volume RLL ML Secoll Lymphocyte Separation Solution: Density g/ml. 500 ml RLL ML Secoll Lymphocyte Separation Solution: Density g/ml. 100 ml Serana Quality Led and Customer Driven 14
16 Antibiotics Trypsin L-Glutamine G418 ANTIBIOTICS TRYPSIN L-GLUTAMINE G418 Product Description Product Information Storage Size This sterile-filtered solution contains 10,000 units/ml of penicillin, 10,000 RAL ML gram-negative bacteria. Suitable for use in cell culture. Penicillin-Streptomycin-Glutamine µg/ml streptomycin and 29.2 mg/ml L-glutamine in 0.9% saline. Penicillin- Solution 100X Streptomycin-Glutamine solution is effective against gram-positive and -5 to -20 C 100 ml RAL ML Penicillin-Streptomycin Solution 100X This sterile-filtered solution contains 10,000 units/ml of penicillin and 10,000 µg/ml streptomycin in 0.9% saline. Penicillin-Streptomycin solution is effective against gram-positive and gram-negative bacteria. Suitable for use in cell culture. -5 to -20 C 100 ml RAL ML Gentamicin Solution (10mg/ml) Contains 10mg/ml gentamicin. Sterile filtered solution. 2 to 8 C 10 ml RAL ML Gentamicin Solution (50mg/ml) Contains 50mg/ml gentamicin. Sterile filtered. 2 to 8 C 10 ml RGL ML L-Glutamine Solution 100X L-glutamine (29.2 mg/ml, 200mM) in 0.85% sodium chloride solution. Sterile filtered. 20 to -5 C 100 ml RAL ML Amphotericin B Solution Contains 250µg/ml amphotericin B and 250µg/ml sodium deoxycholate. Effective against fungi and yeasts. Sterile-filtered solution. -5 to -20 C 100 ml RTL ML Trypsin-EDTA Solution (1X) Contains 0.25% trypsin (from Porcine pancreas) and 1mM EDTA in Hank's Balanced Salt Solution without calcium and magnesium. Sterile filtered. 0 to -20 C 100 ml Porcine parvovirus and mycoplasma tested. RTL ML Trypsin-EDTA Solution 0.5% (10X) Contains 0.5% trypsin (from Porcine pancreas), 5.3mM EDTA, and 8.5 g/l NaCl. No phenol red. Sterile filtered. Porcine parvovirus and mycoplasma 0 to -20 C. 100 ml tested. RTL ML Trypsin-EDTA Solution 0.05% (1X) Contains 0.05% trypsin (from Porcine pancreas) and 1mM EDTA in Hank's Balanced Salts Solution without calcium and magnesium. Sterile filtered. 0 to -20 C 100 ml Porcine parvovirus and mycoplasma tested. RTL ML Trypsin Solution 2.5% (10X) Contains 2.5% trypsin (from Porcine pancreas) and 8.5 g/l sodium chloride. Sterile filtered. Porcine parvovirus and mycoplasma tested. 0 to -20 C 100 ml RGL ML Stabilized L-Glutamine Solution 200 mm (100X) L-alanyl-L-glutamine in 0.85% Sodium Chloride. Sterile filtered. -20 to -5 C 100 ml RGP G G418 disulfate G418 disulfate salt, an aminoglycoside, is used as a selection agent in eukaryotic cells containing the neomycin phosphotransferase gene. CAS Number: ; Formula: C20H40O10N4 2H2SO4; Formula Weight: ; Assay (on dried basis): > 700 U/mg. 2 to 8 C 10 g Serana Quality Led and Customer Driven 15
17 Sterile Buffer Solutions BUFFERS Product Description Product Information Storage Size BHL ML Hank's Balanced Salts HBS solution with calcium, magnesium, and phenol red. Sterile filtered. RT 500ml BHL ML Hank's Balanced Salts With phenol red. Without calcium and magnesium. Sterile filtered RT 500ml BHL ML Hank's Balanced Salts With calcium and magnesium. Without phenol red. Sterile filtered. RT 500ml BDL ML Dulbecco's PBS Without calcium and magnesium. Sterile filtered. RT 500ml BDL ML Dulbecco's PBS (10X) 10X D-PBS without calcium and magnesium. RT 500ml BDL ML Dulbecco's PBS With calcium and magnesium. RT 500ml BHL ML HEPES Solution 1M solution (238.3 g/l HEPES) in purified water. Sterile filtered. RT 100ml BWL ML Purified Water 18 mega-ohm, purified water. Cell culture tested. Sterile Filtered. RT 500ml BPL ML Sodium Pyruvate Solution 100 mm solution (11 mg/ml) in purified water. Sterile filtered. 2 to 8 C 100ml BBL ML Sodium Bicarbonate Solution 7.5% solution (75 mg/ml) in purified water. Sterile filtered. RT 100ml Serana Quality Led and Customer Driven 16
18 Headquarters Germany Serana Europe GmbH Dorfstrasse 5 (Sales) Dorfstrasse 17A (Warehouse) Pessin Tel: (+49) Fax: (+49) info@serana-europe.com Australia Serana (WA) Pty Ltd 6 Juniper Way Bunbury WA 6230 Serana Quality Led and Customer Driven 17
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