Hesperidin. Keep Health, Keep Happiness
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1 Hesperidin Kang-Hui Keep Health, Keep Happiness
2 Table of Contents 01 Brief Introduction 03 Functions 02 Technical Description 04 Experimental Data 05 Product Application 06 Highlights
3 Brief Introduction of Company Sichuan Guangsong Pharmaceutical Co., Ltd is a statedowned GMP company to process natural flavonoid compounds by experienced hydrolysis process which completely accord with the requirement of enviromental protection. Standard factory, complete production equipment and strict GMP management have provide strong support to produce highquality products. Testing the heavy metal, pesticide residue and PAHs by Eurofins regularly, accepting the audits from European, American and Japanese companies per year.
4 Brief Introduction Hesperidin is a flavan-on glycoside found in citrus fruits. Its aglycone form is called hesperetin. Both hesperidin and its aglycone Hesperitin have been reported to possess a wide range of pharmacological properties. Hesperidin, Rutin and other flavonoids thought to reduce capillary permeability and to have anti-inflammatory action were collectively known as vitamin P. Therefore, it was widely used in food, supplements, pharmaceutical, cosmetics and other industries. Laws and Regulations: Hesperidin has been listed in Japan Food Additives List with No.289. Japanese Standards For Raw Materials of Cosmetics with No
5 Technical Description Product Name Hesperidin IUPAC Name 7-(2-o-(6-deoxy-alpha-l-mannopyranosyl)-beta-dglucopyranosyloxy)-2,3-dihydro-4',5,7-trihydroxyflavone Botanical source Orange and Lemon Formula C28H34O15 Molecular Weight CAS NO Specifications 90%, 95% Appearance Off-white, Canary yellow or light brown powder Method HPLC Melting point and solubility Melting point of gram dissolves in 50 liters of water. Slightly soluble in methanol and hot glacial acetic acid, almost insoluble in acetone, benzene and chloroform, but soluble in dilute alkali and pyridine.
6 COA of 90% Hesperidin
7 Functions Nutraceutical: Preclinical studies and clinical trials demonstrated therapeutical effects of hesperidin and its aglycone hesperetin in various diseases, such as neurological disorders, psychiatric disorders and cardiovascular diseases and others, due to its antiinflammatory, antioxidant, lipid-lowering and insulin-sensitizing properties. 1.Superoxide-scavenging ability on free radicals, and inhibition on lipid peroxidation of erythrocyte membrane induced by hydroxyl radical. It has good antioxidant effect to delay aging and protection against aging related diseases. 2. It is anti-inflammatory by suppressing the incremental levels of extracellular signal regulatory enzyme and tumour necrosis factor (TNF). 3. Inhibition on inflammation with acute and chronic, significantly for arthritis. 4. Improvement on the symptoms of diabetic gangrene, meanwhile, it can reduce the blood sugar level (BSL), increase the activity of hepatic glycokinase, and prevent the polysaccharides.
8 Functions 5. Improvement on the activity of the superoxide dismutase of ligand, antioxidant activity, and anti- proliferation of free radical. It also can prevent the Intestinal tumor by improving the microbial community composition of the intestinal tract. 6. It has positive effect on preventing hyperlipidemia, lowering blood lipid, blood pressure and cholesterol in the serum, protecting capillary vessels to prevent micrangium from variceal bleeding, which can be used as drugs for the treatment of hypertension and myocardial infarction. 7. Effectively cure cough and reduce phlegm, it works well on the prevention of acute and chronic bronchitis, cough with copious sputum and other related diseases. 8. Fighting against with fatal shock caused by endotoxin, and reducing the bacteria from liver and spleen (L&S) for preventing liver disease.
9 Functions Cosmetics: It has the effect of whitening by reducing tyrosinase activity, melanin content and cell proliferation rate. It is anti-aging and anti-oxidation by improving the superoxide dismutase (SOD) activity and reducing malondialdehyde (MDA) content. It is antiallergic. Improve the microcirculation around eyes and remove the black eyes.
10 Experimental data The effects of hesperidin on inflammatory cytokines secreted by Raw 264.7(mean value ± standard deviation) with following table: Groups Content of TNF-α (pg/ml) Content of IL-6 (pg/ml) Blank group 5.4± ±0.6 Model group 19.2± ±7.6 High concentration of hesperidin Moderate concentration of hesperidin Low concentration of hesperidin 7.8± ± ± ± ± ±5.4 The results showed that hesperidin can significantly reduce the secretion of inflammatory cytokines of macrophages in abdomen, which indicated that hesperidin with high concentrations had good antiinflammatory activity in vitro.
11 Experimental data Effect of Hesperidin on Serum Tyrosinase Content: Groups Tyrosinase Content (pg/ml) Contrast group 281.6±46.3 Low dose of hesperidin 236.5±32.8* High dose of hesperidin 212.4±28.1* Based the conclusion on above sheet, compare with the contrast group ( normal saline), whether high or low dose of hesperidin, the granular positive cells contains melanin and tyrosinase content of skin were decreased, which indicated that hesperidin has whitening effect. 11
12 Experimental data Anti-aging and Antioxidation: Groups SOD activity (U/mg) MDA Content (nmol/mg Contrast group 114.6± ±0.85 Model group 68.2±9.6** 4.68±1.19** Low dose of hesperidin 92.3± ±0.93 High dose of hesperidin 98.7± ±0.96 Based the conclusion on above sheet, compare with the contrast group ( normal saline), whether high or low dose of hesperidin, the SOD activity was significantly higher than contrast group, conversely, MDA content was much lower, which indicated that hesperidin had anti-aging and antioxidant effect. Anti-allergy (Ear-swelling test): Groups Swelling degree(mg) Swelling inhibition rate(%) Model group 2.28± Low dose of hesperidin 1.76± % High dose of hesperidin 1.68± % Based the conclusion on above sheet, compare with the contrast group ( normal saline), whether high or low dose of hesperidin, it had obvious effect on reducing the ear-swelling reaction. 12
13 Product Applications The main functions of Doctor's Best are to protect the heart and blood vessels from oxidation, boost the immune system, strengthen blood vessel walls and capillaries. The main functions of Country Life are to prevent free radicals and promote the stability of HDL cholesterol. The main functions of Thorne Research's are to support vascular health and stabilization. 13
14 Certificates Certificates of Sichuan Guangsong Pharmaceutical Co., Ltd HALAL ISO KOSHER GMP
15 Highlights 1. Sichuan Guangsong Pharmaceutical Co., Ltd, a state-controlled manufacturers which was established in 1993, Sichuan Medical and Health Products Import & Export Corporation holds 43.10%, legal representative of Chengdu Kanghui biotechnology company holds 42.10%, Pine-PU pharmaceutical company (Japan) holds 14.8%. There is no one quality problem with our products over 20 years which makes a good reputation. 2. We have been successively issued GMP certificate by SFDA, at the same time, we have obtained KOSHER, HALAL and ISO. Furthermore, we will grant FSSC ( contains HACCP) within Our products can be fully conformed to Japanese Standards on heavy metals, pesticide and PAHs, etc which have exported to Japan per year with more than 300 tons rutin as well as accepted the audits from Japanese companies every year. 4.Our company has independent R&D, GMP Laboratory and testing center to meet the requirement of company's production quality control.
16 Kang-Hui Keep Health, Keep Happiness Add:No.98, Middle Huanghe Rd, Xihanggang, Shuangliu, Chengdu, China Tel: Fax: Web:
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