Safe Operating Procedure

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1 Safe Operating Procedure SEWER DISPOSAL LIST This document specifies certain conditions where dilute chemicals may be disposed to the sanitary sewer. This is only applicable to Eckerd College. Disposal may only be made to a sanitary sewer, and not to a septic system or storm sewer. Only aqueous solutions containing only the chemicals listed here may be disposed to a sanitary sewer. Solutions containing chemicals not included on this list, regardless of concentration, may not be disposed to the sanitary sewer. A maximum of 1 liter (before dilution or neutralization) of any specific chemical may be placed down an individual drain each day. Further instructions regarding dilution and/or neutralization that must be followed prior to disposal are described below for some chemicals. o For neutralization, follow any acceptable lab procedures regarding neutralizing the chemical. o Dilution is based on volume and must be made with water. o The entire amount of the solution following treatment may be disposed at one time, even if the resulting total volume is greater than 1 liter. The ph of all chemicals must be in the range of 5.5 to 9.5 before disposal. Water must be run down the drain for a minimum of 5 minutes after each disposal incident. For technical assistance, please call Fawn Crotty X 8447 prior to disposal. Further Instructions Chemical Liquids Only Acetylglucosamine Must be neutralized Acetylsalicylic acid Adenosine Agar Agarose Albumen Must be neutralized, Alconox alginic acid Alumina Aluminum oxides Aluminum silicate (12/01)

2 Aluminum sodium sulfate Must be neutralized, Aluminum sulfate (if acidic) Amino acid Must be neutralized, Aminoacetic acid Ammonium bicarbonate Ammonium carbonate Ammonium chloride Ammonium citrate Ammonium lactate Ammonium phosphate Ammonium stearate Ammonium sulfate Amylopectin Amylose Dilute 20;1 Apyrase Arabinose Must be neutralized, Ascorbic acid Azocoll Becto Agar Beef extract Bees wax (if solubilized) Bentonite Biotin Bis-Tris-Propane B-Nicotinamide adenine dinucleotide phosphate B-NADP Brilliant BlueR Bromocresol Green Brpmpphenol Blue Calcium acetate Calcium borate Must be neutralized Calcium carbonate Calcium chloride Calcium citrate Calcium fluoride Calcium gluconate Calcium glycerophosphate Calcium lactate Calcium pantothenate Calcium phosphate Calcium sulfate

3 Must be neutralized, Dilute Carotene Casein Celite Cellobiose CP Cellulase Cellulase Azure Cellulose Cellulose phosphate Cellulose acetate Cesium Chloride Chlorophyll Cholesterol Choline Choline chloride Citric acid Corn oil Creatinine Cysteine Cytosine Deoxyribonuclease Dextran Dextrin Dextrose D(+) Galactose D(+) Lactose Lactate Standard D(+) Raffinose D(+) Trehalose Diaminobenzidine D-Gluconic acid D-Glycogen (Beef liver) DL-Ethionine DL-Isocritric acid, trisodium salt L-Isoleucine DL-Lactic acid (liquid) D-Mannitol D-Mannoheptose D-Mannosamine Alpha D-Mannose Pentaacetate D-Mannose 99% Mixture of Anomers Marine Broth Dodecyl-agarose resin D-Pantothenic acid D-Sorbitol

4 Endo Agar Epsom salts Ethanol (less than 24%) Ethylene glycol Ferric citrate Ferrous ammonium sulfate Fibrous Cation Exchanger Ficoll Type 400DL Flavin Mononucleotide Folic Acid Fructose Freund s adjuvant Fumaric acid Fullers earth Galactose Gelatin Globulin Must be neutralized, Dilute Gluconic acid Glucose-6-phosphate Glucose standard solution Must be neutralized, Dilute Glutamic acid Glutamine Must be neutralized, Dilute Glutmamic acid Glutathione Glycerol Glycerophosphate Glycine Glycogen Type II from Oyster Glycylglycine Must be neutralized, Dilute Guaiacol GN Broth, Hajna Guanosine Gypsum Hemoglobin Heptahydrate Iron (11,111)Oxode Must be neutralized, Dilute Hydrochloric acid 20:1 Hydroxy-proline Must be neutralized, Dilute Hydroxide

5 20:1 Hypoxanthine Insulin Must be neutralized, Dilute Isoleucine Isomaltose Kaolin Keratin L(-) Maolic acid Must be neutralized, Dilute Lactic acid Lactose Lactulose Lanolin Lecithin Leucine Must be neutralized, Lithium carbonate Lithium chloride Litmus L-Methionine L-Threonine Magnesium borate Must be neutralized, Magnesium carbonate Magnesium citrate Magnesium lactate Magnesium phosphate Magnesium sulfate Malt extract Maltose MacConkey Agar Base (Dehydrated) MacConkey Broth Magnesium Chloride, Hexahydrate Magnesium Sulfate, (Anhydrous) Maganesium sulfate Heptahydrate Mannitol MES Methionine Methyl lactate Dilute if Nonfat Dry Milk Milk, Nonfat Dry Niacinamide

6 Beta-Nicrotinamide adenine dinucleotide beta- NAD Nicotinamide Must be neutralized, Nicotinic acid Nitro blue tetrazolium (~steck solution) Nitroblue tetrazolium Nitroglycerine/dextrose solutions (partially administered) Palmitate Pancreatin Papain Pepsin ph Buffer Solution ph10.0 PH Buffer Solution ph7.0 Phenyl beta D-galactoside Phosphate Monobasic Must be neutralized, Phosphoric acid Polyethylene glycol PEG Polyethylene glycol (MW 4000) Potassium acetate Must be neutralized, Potassium acid phosphate Potassium bicarbonate Potassium bisulfate Potassium bitartrate Potassium borate Potassium bromide Must be neutralized, Potassium carbonate Potassium chloride Potassium citrate Potassium hydrogen phthalate Dilute 20;1 Potassium hydroxide Potassium iodide Potassium lactate Potassium phosphate Potassium phosphate dibasic Potassium phosphate monobasic Potassium Phthalate ph Buffer 4.0 Potassium pyrophosphate Potassium sodium tartrate Potassium sulfate Must be neutralized, Potassium sulfite Potassium tetraborate

7 Potassium tetrathionate Pyridoxine Pyruvic acid methyl ester Rhamnose Riboflavin Riboflavin-5-phosphate Ribonucleic acid Rose bengal dye Salicin Dilute 20;1 Salicin 99% Must be neutralized, Salicylic acid Saponin Sodium acetate Sodium ammonium phosphate Sodium benzoate Sodium bicarbonate Must be neutralized, Dilute Sodium bisulfite Sodium borate Sodium bromide Must be neutralized, Dilute Sodium carbonate Sodium chloride Sodium citrate Sodium dodecyl sulfate Must be neutralized, Dilute Sodium formate Must be neutralized, Dilute Sodium Hydroxide 20:1 Sodium iodide Sodium lactate Sodium lauryl sulfate Sodium molybdate Sodium phosphate Sodium phosphate dibasic anhydrous Sodium phosphate monobasic monohydrate Sodium phosphate tribasic Dilute 20;1 Sodium potassium tartrate Sodium salicylate Sodium sulfate (anhydrous) Sodium tartrate Sodium thiosulfate

8 Sodium trimetaphosphate Must be neutralized, Dilute Sodium tungstate Sorbitol Sorbose Starch Starch agar Stearate Must be neutralized, Dilute Stearic acid Must be neutralized, Dilute Strontium carbonate Strontium chloride hexahydrate Must be neutralized, Dilute Succinic acid Sucrose Sulfate Must be neutralized, Dilute Sulfuric acid 20:1 Must be neutralized, Dilute Tartaric acid Thiamine Thiamine hydrochloride Thymidine (crystalline) Tin oxide Trehalose Tricine Tricalcium phophate Triethanolamine hydrochloride Triethylene hydrochloride Trisodium phosphate Triton X Trypsin (from Bovine pancreas) Tryptone-bacto Uracil Urea Uridine Valine Vanadyl sulfate (hydrate) Must be neutralized, Vanillic acid Vanillin Violet red-blue agar Xylan (from birchwood)

9 Yeast extract Zinc chloride Zinc phosphate Zinc sulfate

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