Feed Ingredient Utility. Mika Alewynse Division of Animal Feeds Center for Veterinary Medicine FDA
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1 Feed Ingredient Utility Mika Alewynse Division of Animal Feeds Center for Veterinary Medicine FDA
2 AAFCO Definition Process Proposed definition Description/ purpose of the ingredient Rationale for request Use limitations Literature information Feeding trials/ controlled observations
3 What Does the Substance Do? Information sources Other uses Scientific literature Books Internet databases (AGRICOLA) Libraries Traditional research studies Behavior of related substances
4 What Do You Want to Say About Substance X? Technical effect on feed Preservative Emulsifier Pelleting aid Source of something for the animal
5 Types of Substances Intended for the Animal Nutrient Essential nutrients (amino acids, fatty acids, vitamins, minerals, water, and in some cases, fiber) Flavor (taste) Aroma (smell) Coloring regulated process New substance Feed components have effects beyond supplying essential nutrients enzyme
6 Caveats about What You Say DSHEA does not apply to animal feed Federal Register 61:17706 Substance could be both a food and a drug, based on claims made for product CVM Program Policy and Procedures Manual - Guide Some structure/function claims could be acceptable if related to food properties
7 How to Show Substance X Works (Utility) Prior approval or acceptance? Substance listed in CFR approved Food Additives for feed - 21 CFR 573 partial List of Generally Recognized as Safe (GRAS) Substances 21 CFR 582 Feed ingredient definition published in Official Publication of AAFCO If approved or listed for the intended use, no problem Remember intended use includes target species, may need to address this issue
8 Showing Substance X Works (cont.) Recognized by an authoritative organization National Research Council taurine for cats Flavor & Extract Manufacturers Assn (FEMA) Is the intended use the same? Demonstrate that Substance X has food properties Therapeutic effect = animal drug
9 What You Need to Show Dependent on identity and properties of Substance X What is the possible mode of action? Scientific literature Non-therapeutic physiological effect Effects of related compounds Number of needed studies varies
10 Substance X is Nutrient Look in the literature first Use the appropriate animal model Avoid chicks if intended use is cattle Lots of different traditional study designs Deficiency/supplementation studies Use a known comparator Account for bioavailability
11 Substance X is Flavor/Aroma Primarily pet food, but young animals, too Use the appropriate animal model Cats and dogs are very different Be sure to use sufficient animals and choices Subjects need multiple bowls/feeders If using older subjects, be aware of Prior exposure Predispositions/conditioning Consult with experts as palatability/food choice studies are tricky
12 New Substance What is the intended use? Many of these substances fall in a gray area See CVM Program Policy and Procedures Manual - Guide Improved productivity = drug Disease prevention/treatment/mitigation = drug Use appropriate measures for feed ingredient
13 Designing the Study You need statistics! Foreign locations can be used if conditions are like those in US Sound study design Use sufficient experimental numbers (power of test) Focus on effect of Substance X studies often include too many additional factors
14 Study Design (cont) Mimic proposed conditions of use Target species cattle versus chickens animal class Purpose statement in AAFCO Reg. 3 Test proposed use rate! if test down to 500 mg/ lb, label use rate cannot be 250 mg/ lb if test up to 4000 ppm, label use cannot be for 5000 ppm
15 Study Design (cont) Pelleted versus mash diets Free choice versus limit feeding Data must support claim Measuring fecal Ca levels does not support claims for improved bone density Animal production or disease prevention/treatment data can mean Substance X is an animal drug!
16 Study Conduct Analyze feed for Substance X Do not assume it is there Clearly explain how activity relates to amount added to feed Account for: Bioavailability Storage/ dietary stability When study protocol is not followed, explain the changes Don t make us guess
17 Study Results Appropriate statistical analysis Use the CORRECT experimental unit often the pen, not individual animal Make sure you can compare treatments lowest level which shows statistical improvement over negative control contrast of control vs. all levels of substance X unlikely to be beneficial
18 General Points Submit complete study reports Raw data (sometimes) Statistical printouts Be sure submitted data support claim Substance X had a significant effect as expected Data can be pooled over studies if appropriate Submit all information and explain any conflicting studies Not good when we find conflicting studies that are not mentioned
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