Development of fructo-oligasaccharide (FOS) dietary supplement from plants for elders and digestive disorder patients

Transcription

1 Development of fructo-oligasaccharide (FOS) dietary supplement from plants for elders and digestive disorder patients Prof.Dr. Jiradej Manosroi Prof.Dr. Aranya Manosroi Manose Health and Beauty Research Center 179 Moo 10 Klong, Cholpratan Road, Suthep, Muang, Chiang Mai, 50200, Thailand 1

2 FRUCTO-OLIGASACCHARIDE (FOS) MANOSÉ» Fructooligosaccharide (FOS) is low calorie complex sugar and water soluble oligosaccharide. It is a prebiotic which can be completely digested in human body.» FOS can stimulate the growth of beneficial intestinal microflora such as Lactobacillus spp. and Bifidobacteria spp., reduce constipation, reduce the risk of colon cancer, immunostimulation in intestinal tract, improved function and health of intestinal tract.» FOS compose of glucose (G) and fructose (F) molecules linked by DP (degree of polymerization) at 2-9 (DP is the number of glucose and fructose molecules). 2

3 Figure 1 Main chemical structure of FOS 3

4 Plants for FOS extraction MANOSÉ - 13 plants contained with FOS are red onion, water chestnut, onion, dragon fruit, snow lotus, garlic, sweet potato, radish pumpkin, mango, tomato, banana and grains 4 Figure 2 Example of plants for FOS extraction

5 Analysis of FOS content in the extracts Note: K; 1-kestose, N; nystose, FN; 1-β-D-fructofuranosylnystose Figure 3 HPLC and Refractive Index (RI) detector Figure 4 Chromatogram of FOS standard - FOS extracts from red onion, radish and grains gave the total FOS contents at 83.84±4.96%, 32.17±19.16% and 24.98±12.95%, respectively. 5

6 Anti-oxidative activity test FOS extract from grains showed free radical scavenging activity at 0.92 folds of vitamin C and lipid peroxidation inhibition activity at 0.01 folds of vitamin E Figure 5 Anti-oxidative activity test 6

7 Growth stimulation study on human skin fibroblast Figure 6 human skin fibroblast cell line CELL: Human skin fibroblast TYPE: Epithelial SOURCE: SKIN MEDIUM: MEM Alpha Modification FROM: Department of Tropical Hygine, Faculty of Tropical Medicine, Mahidol University, Thailand. 7

8 Sulforhodamine B (SRB) colorimetry assay FOS extracts from radish, red onion and grains showed growth stimulated on human skin fibroblast at 1.10, 1.02 and 1.17 folds of vitamin C, respectively. 8

9 Development of dietary supplement MANOSÉ product containing with FOS extract 1. FOS extraction in pilot scale Figure 7 FOS extraction in pilot scale Figure 8 FOS extract from grains 9

10 2.Acute toxicity study in animal Organ Control FOS 2,000 µg/kg FOS 5,000 µg/kg Liver Kidney s FOS extract from grains showed no changes of histopathology of liver, kidneys and spleen of animals including biochemistry parameters such as creatinine, AST, ALT and total bilirubin. Spleen 10 Figure 9 Histopathology of animal internal organs from control group and FOS extract treated group at 2,000 and 5,000 µg/kg (magnification at 40 X)

11 3. Preparation of dietary supplement Figure 10 Extract and dietary supplement of FOS extract from grains The granule containing FOS extracts from grains showed passable flow and fair compressibility. 11 Figure 11 Equipments for compression and flow ability test of dietary supplement product

12 Efficacy and satisfaction studies in volunteers Efficacy and satisfaction in averaged 35 years volunteers for 6 weeks showed that dietary supplement containing FOS extract gave no side effects, no change in the bodyweight of volunteer and relieved in digestive and intestinal disturbances. 12 Figure 12 Investigation of efficacy and satisfaction in volunteer

13 Conclusion The FOS extract from grains gave high anti-oxidative activity similar to vitamin C with no toxicity to human skin fibroblast. The developed dietary supplement containing FOS extract from the grains has advantage such as anti-oxidative activity comparing with commercial products. It was produced from Thai plant which was cheaper than the imported FOS dietary supplements. Furthermore, It also have high efficacy for relieving digestive disorder. Hence, FOS extract has high potential to be developed to the dietary supplements and cosmetic products due to its prebiotic and anti-oxidative activities, respectively. 13

14 Acknowledgements The investigators would like to thank The Thailand Research Fund (TRF), QualiMed company and Natural Products Research and Development Center (NPRDC), Science and Technology Research Institute (STRI), Chiang Mai University for the financial supports of this work. 14