ViTALS Tests and Fees Effective: 9/1/17

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1 Provide puple top whole blood from the donor, ensure tubes are well-labeled as to Blood Bank Crossmatch - Gel donor and recipient Blood Bank DEA 1.1 Blood Type Blood Bank Feline Blood Type BG SYRINGE, GREEN TOP LI HEP, maintain sample in Blood Gas Ionized Calcium Ionized Calcium anaerobic conditions, do not expose to air. Body Fluid Albumin, Body Fluid Globulin, Body Fluid Body Fluid TP/ALB/GLOB Body Fluid Total Protein RED TOP NO ADDITIVE Body Fluid Body Fluid ALKP Body Fluid ALKP RED TOP NO ADDITIVE Body Fluid Body Fluid Amylase Body Fluid Amylase RED TOP NO ADDITIVE Body Fluid Body Fluid Calcium Body Fluid Calcium RED TOP NO ADDITIVE Body Fluid Body Fluid Chloride Body Fluid Chloride RED TOP NO ADDITIVE Body Fluid Body Fluid Cholesterol Body Fluid Cholesterol RED TOP NO ADDITIVE Body Fluid Body Fluid Creatinine Body Fluid Creatinine RED TOP NO ADDITIVE Body Fluid Body Fluid Glucose Body Fluid Glucose RED TOP NO ADDITIVE Body Fluid Body Fluid Lipase Body Fluid Lipase RED TOP NO ADDITIVE Body Fluid Body Fluid Magnesium Body Fluid Magnesium RED TOP NO ADDITIVE Body Fluid Body Fluid Phosphorous Body Fluid Phosphorous RED TOP NO ADDITIVE Body Fluid Body Fluid Potassium Body Fluid Potassium RED TOP NO ADDITIVE Body Fluid Body Fluid Sodium Body Fluid Sodium RED TOP NO ADDITIVE Body Fluid Total Bilirubin, Body Fluid Direct Body Fluid Body Fluid Total Bilirubin Bilirubin, Body Fluid Indirect Bilirubin RED TOP NO ADDITIVE Body Fluid Body Fluid Triglyceride Body Fluid Triglyceride RED TOP NO ADDITIVE ALB Albumin ALKP ALKP ALT ALT (GPT) AMMONIA Ammonia ON ICE AMYL Amylase PANC Amylase, Lipase AST AST (GOT) Prices are subject to change without notice, contact Lab Central Receiving at for current pricing. Page 1 of 9

2 PreBA Bile Acids (Fasting) PostBA Bile Acids (Post) CA Calcium CL Chloride CHOL Cholesterol 9.00 CK CK CO2 CO CREAT Creatinine D3HB D3HB GGT GGT GLU Glucose FISH PROFILE Glucose, Creatinine, Phosphorus, Calcium, Magnesium, Total Protein, Albumin, AST (GOT), ALKP, Total Bilirubin, Direct Bilirubin, Indirect Bilirubin, Cholesterol, Sodium, Potassium, Chloride Prices are subject to change without notice, contact Lab Central Receiving at for current pricing. Page 2 of 9

3 HYPERTHY Panel RENAL Panel Large Animal Profile Small Animal Profile Glucose, Urea Nitrogen, Creatinine, ALT (GPT), ALKP Glucose, Urea Nitrogen, Creatinine, Phosphorus, Calcium, Albumin, Cholesterol, Sodium, Potassium, Chloride, CO2, Anion Gap Glucose, Urea Nitrogen, Creatinine, Phosphorus, Calcium, Magnesium, Total Protein, Albumin, Globulin, AST (GOT), GGT, Total Bilirubin, Direct Bilirubin, Indirect Bilirubin, CK, Sodium, Potassium, Chloride, CO2, Anion Gap Glucose, Urea Nitrogen, Creatinine, Phosphorus, Calcium, Total Protein, Albumin, Globulin, ALT (GPT), ALKP, GGT, Total Bilirubin, CK, Cholesterol, Triglycerides, Sodium, Potassium, Chloride, CO2, Anion Gap CHEM 8 AU480 Glucose, Urea Nitrogen, Creatinine, Sodium, Potassium, Chloride, CO2, Anion Gap PREOP Panel Glucose, Urea Nitrogen, Creatinine, Total Protein, Albumin, Globulin, ALT (GPT), ALKP, Sodium, Potassium, Chloride MOUSE PROFILE Glucose, Urea Nitrogen, Creatinine, Total Protein, ALT (GPT) BOVINE PROFILE Glucose, Urea Nitrogen, Phosphorus, Calcium, Magnesium, CK, Sodium, Potassium, Chloride, CO2, D3HB LIVER Glucose, Urea Nitrogen, Total Protein, Albumin, Globulin, ALT (GPT), ALKP, GGT, Total Bilirubin, Cholesterol LIPASE Lipase MG Magnesium NEFA NEFA RED TOP NO ADDITIVE ONLY: centrifuge sample & transfer supernatant (serum) to a sterile container (red-top or plastic tube) before shipping Prices are subject to change without notice, contact Lab Central Receiving at for current pricing. Page 3 of 9

4 PHOS Phosphorus MIN Phosphorus, Calcium, Magnesium Phosphorus, Calcium, Magnesium, Total Protein, Albumin, Globulin, AST (GOT), GGT, Total Bilirubin, Direct Bilirubin, Indirect Large Animal Mini Profile Bilirubin CHICKEN PROFILE Small animal mini profile Phosphorus, Calcium, Magnesium, Total Protein, ALT (GPT), AST (GOT), Total Bilirubin, Direct Bilirubin, Indirect Bilirubin, CK, Sodium, Potassium, Chloride Phosphorus, Calcium, Total Protein, Albumin, Globulin, ALT (GPT), AST (GOT), ALKP, GGT, Total Bilirubin, Direct Bilirubin, Indirect Bilirubin, Cholesterol K Potassium NA Sodium LYTES Sodium, Potassium, Chloride, CO2, Anion Gap SDH Sorbitol dehydrogenase RED TOP NO ADDITIVE ONLY: centrifuge sample & transfer supernatant (serum) to a sterile container (red-top or plastic tube) before shipping TBIL Total Bilirubin, Direct Bilirubin, Indirect Bilirubin TP Total Protein ALB/GLOB/TP Total Protein, Albumin, Globulin Prices are subject to change without notice, contact Lab Central Receiving at for current pricing. Page 4 of 9

5 TRIG Triglycerides BUN Urea Nitrogen Urea Nitrogen, Creatinine, Total Protein, ALT NSAID Panel (GPT), ALKP, Total Bilirubin Coagulation PT PT BLUE TOP NA CITRATE Coagulation PTT PTT BLUE TOP NA CITRATE Fluid without counts Examples: Bronchoaveolar lavage, tracheal wash, urine, bile, seminal/prostatic fluid, fluid from mass, washes/flushes, pus or abscess RED TOP or +/- smear slides of the fluid with ice packs Fluid with counts Examples: Pleural effusion, thoracic fluid, peritoneal fluid, abdominal cavity fluid, pericardial effusion/fluid +/- smear slides of the fluid with ice packs Cerebrospinal Fluid (CSF) Includes nucleated & red cell counts, protein, & cytologic evaluation RED TOP NO ADDITIVE +/- smear slides with ice packs Bone Marrow Path Review Pathologist reviews the blood smear. must be accompanied by a CBC that has been performed within 24hours (CBC may be performed at additional charge) Slides + or Unstained slides +/- Whole blood. Include recent (<48 hours) CBC or for CBC (additional charge applies) with ice packs with ice packs Buffy Coat smear slide made from the layer separating the plasma from the packed cells Basic - Smears Smear Slides labeled with patient name and site Slides in protective slide holder Lymph node, single site Slides, labeled with patient name and site Slides in protective slide holder Lymph node, multiple sites Slides, labeled with patient name and site(s) Slides in protective slide holder Analyzer blood counts only- No blood smear Hemogram Equine review/manual cell count Analyzer blood counts only- No blood smear Hemogram Large Animal review/manual cell count Analyzer blood counts only- No blood smear Hemogram Small Animal review/manual cell count Hematocrit Hematocrit 9.00 Hemoglobin Hemoglobin Manual Reticulocyte Count, Manual Manual Reticulocyte Count Reticulocyte Absolute Packed Cell Volume Packed Cell Volume 9.00 Packed Cell Volume, Plasma Protein, Plasma PCV/TP Appearance Packed Cell Volume, Plasma Protein, PP57, PCV/TP/FIB Fibrinogen, Plasma Appearance Prices are subject to change without notice, contact Lab Central Receiving at for current pricing. Page 5 of 9

6 Plasma Protein Plasma Protein 9.00 Fibrinogen, PP Plasma Protein, Fibrinogen 9.00 Platelets, Slide Platelet Estimate, Platelets Platelet Count Clumped, Platelet Morphology RBC RBC Submit smear slides made from EDTA--WB at CBC Equine time of draw + 2 smear slides Submit smear slides made from EDTA---WB CBC Large Animal at time of draw + 2 smear slides Submit smear slides made from EDTA---WB CBC Small Animal at time of draw + 2 smear slides WBC WBC Immunology Clostridium Perfringens Enzyme Immunoassay for enterotoxin detection Feces Submit within 2 hours of collection Immunology Clostridium Difficile Enzyme immunoassay for the detection of toxins A & B produced by toxigenic strains of C. difficile Feces Submit within 2 hours of collection Immunology Brucella canis Slide agglutination test RED TOP NO ADDITIVE (1) Immunology FELV,FIV Combo SNAP Test Purple, green or red top tube Immunology SNAP 4Dx SNAP Test Purple, green or red top tube Immunology Camelid IgG (RID) RED TOP NO ADDITIVE (1) Separate serum within 2 hours of collection Acid Fast Stain out Anaerobic Culture Anaerobe swab, if a swab is used. For fluids, submit in a container with all air removed, such as a syringe. For tissues, as large a piece as possible in a sterile container. Anaerobes die quickly in the presence of air. out Blood Culture Standard blood collection bottles only Hand deliver/courier, contact lab Fecal Screen Culture Feces Overnight, Cary Blair transport media at room temperature is best Fungal Culture (Mycology) Gram Stain MRSA/MRSI/MRSP Culture Screen Routine (Aerobic) Culture + Sensitivity Only screens for Methycillin-resistant Staphylococcus spp. Culture swabs/culturettes preferred; Fluids, & tissue. Urine preferred submission: sterile container or red top tube out out out out Prices are subject to change without notice, contact Lab Central Receiving at for current pricing. Page 6 of 9

7 Salmonella Culture out Strep Equi Screen Culture Feline Coronavirus/FIP PCR out Will accept but not validated for: ascites fluid, 0.5mL (red top tube) Formalin-fixed Paraffin Embedded tissue block Freeze liquid samples; ship overnight with ice packs Canine Distemper Virus PCR Bovine Respiratory Syncytial Virus PCR Bovine Viral Diarrhea Virus PCR Bovine Herpesvirus Type 1/IBR PCR Will accept but not validated for: 0.5mL serum (red top tube) or EDTA plasma (purple top tube), conjunctival swabs (dry), urineany method of collection (sterile specimen container or red top tube) Formalin-fixed Paraffin Embedded tissue block Freeze liquid samples; ship overnight with ice packs Will accept but not validated for: lung tissue (whir-pak or sterile specimen container), respiratory swabs (dry), 0.5mL Bronchoaveolar lavage fluid (BAL)(red top tube) Formalin-fixed Paraffin Embedded tissue block Freeze liquid samples; ship overnight with ice packs Will accept but not validated for: nasal swabs (dry) Formalin-fixed Paraffin Embedded tissue block Freeze liquid samples; ship overnight with ice packs Will accept but not validated for: respiratory swabs (dry), 0.5mL Bronchoaveolar lavage (BAL) fluid(red top tube), lung, fetal liver, spleen, placenta, trachea (whir-pak or steril specimen container) Formalin-fixed Paraffin Embedded tissue block Freeze liquid samples; ship overnight with ice packs Salmonella PCR Preferred Matrices: greater than 1.0 mlor gram of fresh feces (preferable), feces in Cary Blair transport media DO NOT REFRIGERATE/FREEZE SAMPLE If shipping, use Cary Blair transport media, do not use ice packs, ship overnight. If using courier, fresh preferred in sterile specimen container, do not use ice packs Heartworm Antibody Test Dirofilaria, cats only Ship samples overnight with ice Heartworm Antigen Test Dirofilaria, dogs & cats Ship samples overnight with ice Knott's Test for Microfilariae Microfilariae (heartworm) Ship samples overnight with ice McMaster Camelid Quantative test when egg counts low (camelids, adult cattle) 2-4g of feces Place fecal sample in an unbreakable, leak-proof container. Exception : For quantitative egg counts, place sample in plastic bags with a sealable top; express air out before sealing bag. Ship samples overnight with ice; prevent direct contact between ice and fecal sample. NOTE: For multiple submissions/herd testing, please contact Dr. Zajac at McMaster Standard Zinc Sulfate Flotation Test Sedimentation Quantitative test for small ruminants, horses, & cattle (cattle <2 years of age) Routine flotation for parasite screening in small animals Small Animal: Fluke eggs Large Animals: Flukes 2-4g of feces 2-5g of feces 5-10g of feces Place fecal sample in an unbreakable, leak-proof container. Exception : For quantitative egg counts, place sample in plastic bags with a sealable top; express air out before sealing bag. Ship samples overnight with ice; prevent direct contact between ice and fecal sample. NOTE: For multiple submissions/herd testing, please contact Dr. Zajac at Place fecal sample in an unbreakable, leak-proof container. Ship samples overnight with ice; prevent direct contact between ice and fecal sample Place fecal sample in an unbreakable, leak-proof container. Ship samples overnight with ice; prevent direct contact between ice and fecal sample. NOTE: For multiple submissions/herd testing, please contact Dr. Zajac at Prices are subject to change without notice, contact Lab Central Receiving at for current pricing. Page 7 of 9

8 Sheather's Sugar Float Baermann Exam Small Animal: Flotation, including tapeworms, Physaloptera Large Animal: Qualitative, Routine screening, Eimeria macusaniensis in camelids, Cryptosporidium Small Animals: Nematode larvae (e.g. Strongyloides, Aleurostrongylus ) *Requires hours Large Animals: Nematode larvae (lungworms) *Requires hrs 2-5g of feces 5-10g of feces Place fecal sample in an unbreakable, leak-proof container. Ship samples overnight with ice; prevent direct contact between ice and fecal sample. NOTE: For multiple submissions/herd testing, please contact Dr. Zajac at Place fecal sample in an unbreakable, leak-proof container. Ship samples overnight with ice; prevent direct contact between ice and fecal sample. NOTE: For multiple submissions/herd testing, please contact Dr. Zajac at Parasite Identification Small Animals: ID of helminth & arthropod parasites (e.g. adult worms, ticks, mites) Large Animals: ID of helminth & arthropod parasites (e.g. adult worms, ticks, mites) Giardia Antigen SNAP Test 5g of Feces Comprehensive evaluation of organ sytemsbacteriology, parasitology, histology included. Some ancillary testing available at additional costs (i.e. toxicology, PCR, etc.)- notify necropsy lab if desired. Pricing is Necropsy based on weight. Submit parasite in leakproof container Carcass- cooled to refrigeration temperatures, if possible. Rabies vaccination status must be provided. Indicate disposal preference on submission form. Place sample in an unbreakable, leak-proof container. Arthropod(s) preserved in 70% Alcohol (preferred) and worms in 10% Formalin (preferred). Ship samples overnight with ice. NOTE: For multiple submissions/herd testing, please contact Dr. Zajac at Place fecal sample in an unbreakable, leak-proof container. Ship samples overnight with ice; prevent direct contact between ice and fecal sample Greater than 300 lbs Less than 300 lbs.- 77 Drop-off: call necropsy to coordinate at Mail: Refer to Necropsy Shipping Requirements, avoid freezing carcasses Histology- Field Necropsy Histology Histological evaluation of issue samples gathered from a field necropsy. Pricing is based on sites or tissue location. Histological evaluation of tissue samples. Pricing based on tissue sites or location of biopsy or excision. Formalin-fixed tissues (1:10 tissue to formalin ratio recommended), leak-proof container (NO GLASS); see Field Necropsy Submission Guidelines Formalin-fixed tissues (1:10 tissue to formalin ratio recommended), leak-proof container (NO GLASS) Overnight with absorbent material, ice packs recommended in summer months. Leaking samples may be assessed a HAZARD FEE. Overnight with absorbent material, ice packs recommended in summer months. Leaking samples may be assessed a HAZARD FEE. Single site- 70; 2-3 sites- 93, 4-9 sites- 120, 10+ sites- 145 Single site- 70; 2-3 sites- 93, 4-9 sites- 120, 10+ sites- 145 Head Heart & Hooves Private Cremation Immunohistochemistry (IHC) Stains shipped to the owner by the crematorium Must be accompanied with a necropsy. see necropsy shipping requirements Formalin fixed paraffin embedded (FFPE) tissues or Unstained, FFPE tissue sections cut at 4-5µm, collected on positively charged slides for a total of 5 IHC Stains offered: CD3, CD18, CD79a, Factor slides per antibody requested. If ViTALS has 8, GFAP, anti-mart-1-melan A, CK-AE1/AE3, performed the histology or necropsy, please contact Vimentin, KI-67, NSE, and Iba-1 the laboratory to coordinate requesting IHC staining. Overnight, for paraffin blocks- ice packs recommended in Pricing is per antibody/per block or slide site summer months; for slides- package to prevent slides requested. from damage during shippment Private Cremation- 41 to 75 lbs. shipped to the owner by the crematorium Must be accompanied with a necropsy. Must provide animal's current weight. see necropsy shipping requirements Private Cremation- 76 to 100 lbs. shipped to the owner by the crematorium Must be accompanied with a necropsy. Must provide animal's current weight. see necropsy shipping requirements Private Cremation- 101 to 150 lbs. shipped to the owner by the crematorium Must be accompanied with a necropsy. Must provide animal's current weight. see necropsy shipping requirements Prices are subject to change without notice, contact Lab Central Receiving at for current pricing. Page 8 of 9

9 Private Cremation- less than 40 lbs. shipped to the owner by the crematorium Must be accompanied with a necropsy. Must provide animal's current weight. see necropsy shipping requirements contact returned to the owner by the crematorium Must be accompanied with a necropsy. Must provide necropsy Private Cremation- Over 150 lbs. using U.S. Postal Service animal's current weight. see necropsy shipping requirements Special Cortisol (Post) Cortisol (Post) Special Cortisol (Pre) Cortisol (Pre) Special Phenobarbital Phenobarbital Special Progesterone Progesterone Special SNAP cpl SNAP cpl RED TOP NO ADDITIVE Special T4 T Special T4, TSH T4, TSH Special TSH TSH Urinalysis Urinalysis Routine Urinalysis w/ specific gravity, fluid anared TOP NO ADDITIVE Urine UAMY Urine Amylase RED TOP NO ADDITIVE Urine UCA Urine Calcium RED TOP NO ADDITIVE 9.00 Urine UCL Urine Chloride RED TOP NO ADDITIVE Urine UCREA Urine Creatinine RED TOP NO ADDITIVE Urine UEQGGT Urine GGT RED TOP NO ADDITIVE Urine UGLU Urine Glucose RED TOP NO ADDITIVE 9.00 Urine UMG Urine Magnesium RED TOP NO ADDITIVE 9.00 Urine UPHOS Urine Phosphorus RED TOP NO ADDITIVE 9.00 Urine UK Urine Potassium RED TOP NO ADDITIVE Urine UPRO Urine Protein RED TOP NO ADDITIVE Urine UNA Urine Sodium RED TOP NO ADDITIVE Urine UBUN Urine Urea Nitrogen RED TOP NO ADDITIVE 9.00 Prices are subject to change without notice, contact Lab Central Receiving at for current pricing. Page 9 of 9