Spanish AMR Monitoring Program in Healthy Animals

Transcription

1 Spanish AMR Monitoring Program in Healthy Animals November 28-11,2016 José Luis Sáez Llorente Ministry of Agriculture and Fish, Food and Environment affairs Animal Health and Hygiene and Traceability Department

2 1. EU Rules: Directive 2003/99 EC, on the monitoring of zoonoses and zoonotic agents Regulation (EC) Nº 2160/2003: on the control of salmonella and other specified food-borne zoonotic agents Decision EU 652/2013 on the monitoring and reporting of antimicrobial resistance in zoonotic and commensal bacteria Technical specifications on the harmonised monitoring and reporting of antimicrobial resistance in Salmonella, Campylobacter and indicator Escherichia coli and Enterococcus spp. bacteria transmitted through food

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4 Bacterias Mandatory: Salmonella spp C. jejuni Indicator commensal E. coli ESBLs, AmpC, carbapenemases-producing Salmonella spp. And E. coli. From 2015 Optional: C. coli Indicator commensal E. faecalis y E. faecium

5 Salmonella spp: Layers, broilers and fattening turkeys ( SNCP) C. jejuni (caecal samples, slaughterhouses): Mandatory Bacterias Broilers and fattening turkeys if production > tonnes slaughtered per year Indicator comensal E. coli (caecal samples, slaughterhouses): Broilers and fattening turkeys if production is more tan tonnes per year Fattening pigs and calves under 1 year if production is more tan tonnes per year ESBL or AmpC or carbapenemase- producing E. coli or Salmonella (caecal samples, slaughterhouses): Broilers and fattening turkeys if production is more tan tonnes per year Fattening pigs and calves under 1 year if production is more tan tonnes per year

6 Optional Bacterias C. coli (caecal samples, slaughterhouses): Broilers Fattening pigs Indicator commensal E. faecalis y E. faecium (caecal samples, slaughterhouses): Broilers and fattening turkeys if production is more tan tonnes per year Fattening pigs and calves under 1 year if production is more tan tonnes per year

7 Spanish Sampling In the years 2014, 2016, 2018 and 2020: Laying hens: Salmonella Broilers: Salmonella C. jejuni, C.coli Indicator comensal E.coli ESBL or AmpC or carbapenemase- producing E. coli Fattening turkeys: Salmonella C. jejuni, C.coli Indicator comensal E.coli ESBL or AmpC or carbapenemase- producing E. coli

8 Spanish Sampling In the years 2015, 2017 and 2019: Calves under 1 year: Indicator commensal E.coli ESBL or AmpC or carbapenemase- producing E. coli, C.jejuni, C.coli Salmonella VTEC Fattening pigs: Indicator commensal E.coli ESBL or AmpC or carbapenemase- producing E. coli C.jejuni, C.coli Salmonella MRSA Yersinia Layers, broilers and fattening turkeys (Salmonella in 2015)

9 Spanish Sampling Random Slaughterhouses processing at least 60% of the specific domestic animal population in Spain Stratified per slaughterhouse During all year Epidemiological unit: Flock in poultry (PNCS) Farm in slaughterhouse 1strain per epidemiological unit and per year

10 Total:15 Slaughterhouses 45,3% National production Broilers Slaughterhouses

11 Total:7 Slaughterhouses 100% national production Fattening turkeys slaughterhouses

12 Total:20 Slaughterhouses 58% National production Fattening pigs slaughterhouses

13 Total:17 Slaughterhouses 58,5% national production Slaughterhouses calves<1 year

14 Spanish Monitoring programme Centralized programme (Only Ministry, no ACs): Coordination and Implementation Reporting data to EFSA Preparing Technical and financial reports (EU Commission) Sampling and some analyses: Call for tender published by The Agriculture Ministry During previous years the winning laboratory was the same: UCM National Reference Laboratory is Algete (belongs to the Ministry)

15 Data Reporting to EFSA Database:

16 Data Reporting to EFSA

17 Data Reporting to EFSA

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19 Campylobacter According to Decision EU 652/2013 on the monitoring and reporting of antimicrobial resistance in zoonotic and commensal bacteria Mandatory Optional

20 Campylobacter According to EFSA Technical specifications on the harmonised monitoring and reporting of antimicrobial resistance in Salmonella, Campylobacter and indicator Escherichia coli and Enterococcus spp. bacteria transmitted through food

21 Percentage Percentage Campylobacter 1. C. coli may be more resistant than C. jejuni 2. C. jejuni is more prevalent in broilers and fattening turkeys; not in Spain: To reach the number of 170 isolates of C.jejuni, it would be necessary to increase the sampling, mainly in turkeys, that is not possible for us 56,79 43,21 Campylobacter broilers 61,05 37,89 51,05 48,25 52,43 47,57 0,00 1,05 0,70 0, c. coli c. jejuni Campy spp Campylobacter fattening turkeys 80,00 20, ,00 c. coli c. jejuni Campy spp

22 Campylobacter We propose a modification of the Technical specifications, and then a modification of the Decision 2013/652 in the following way: C. jejuni would continue as the mandatory Campylobacter specie to be monitored In the case that the MS cannot reach the required number of isolates of 170, testing all of C. jejuni isolated, a number of isolates of the voluntary monitoring of C.coli, randomly selected, could be used to complete the total number of 170 isolates required to perform the AMR testing. Similar approach is followed in the Decision when MS do not obtain 170 isolates of Salmonella spp. by the official sampling. In this case, to complete the number of isolates required the CA can use the FBOs strains in order to reach the 170 isolates.

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