Products for. Microbiology. Microbiology

Transcription

1 Products for Microbiology Microbiology

2 Microbiological Analysis of Water Membrane filtration methods, and other alternative methods Drinking Water Parameter Parametric value Method Recommended product Escherichia coli 0 CFU in 100 ml ISO SCO/778/2009 Tergitol 7 Agar (Chapman TTC modified) (ISO :2000). According to ISO standard. CCA Agar Enterococci 0 CFU in 100 ml ISO Slanetz Bartley Medium (ISO :2000). According to ISO standard. Clostridium perfringens (including spores) 0 CFU in 100 ml 98/83/EC ISO 7937 m-cp Agar TSC Agar Coliform bacteria 0 CFU in 100 ml ISO SCO/778/2009 Tergitol 7 Agar (Chapman TTC modified) (ISO :2000). According to ISO standard. CCA Agar Colony count at 22 C After central treatment From distribution network 100 CFU in 1 ml No abnormal change ISO 6222 Tryptone Yeast Extract Agar (ISO 6222:1999). According to ISO standard. Bottled Drinking Water Parameter Parametric value Method Recommended product Coliform bacteria and Escherichia coli (E. coli) 0/250 ml ISO Tergitol 7 Agar (Chapman TTC modified). (ISO :2000). According to ISO standard. Enterococci 0/250 ml ISO Pseudomonas aeruginosa 0/250 ml EN ISO Slanetz Bartley Medium (ISO :2000) According to ISO standard Pseudomonas CN Agar (EN ISO 16266). According to ISO standard. Colony count at 22 C Incubation 72 hours 100/ml ISO 6222 Tryptone Yeast Extract Agar (ISO 6222:1999). According to ISO standard. Colony count at 37 C Incubation 24 hours 20/ml ISO 6222 Tryptone Yeast Extract Agar (ISO 6222:1999). According to ISO standard. Sulfite reducing clostridia* 0/50 ml SPS Agar Clostridium perfringens (including spores) 0/100 ml 98/83/EC ISO 7937 m-cp Agar TSC Agar *For natural mineral waters and spring waters. 2 Products for Microbiology

3 Microbiology Enumeration of Aerobic Bacteria A Coliforms and Escherichia coli B Tryptone Yeast Extract Agar (ISO 6222:1999) A B D E C Codes and A - Clean drinking water (1 ml). Incubation at 22 C/ 72 hours. Absence/ml. B - Drinking water contaminated with E.coli ATCC (1 ml). Incubation at 22 C/ 72 hours <100 fcu/ml. Enterococci Tergitol 7 Agar (Chapman TTC modified (ISO :2000) Code A B C Slanetz Bartley Medium Code Description: Enterococci form red or reddish brown colonies with a diameter of 1-2 mm. A - Blank Plate. B - Drinking Water (100 ml). Incubation at 37 C/ 24 hours. Absence/100 ml. C - Water contaminated with E. faecalis ATCC (100 ml). Incubation at 37 C/ 24 hours. Presence. Description: Coliform bacteria form yellow colonies or yellow colonies with orange-coloured center colonies or reddish brown inside a yellow halo. A - Water contaminated with S. typhimurium ATCC and P. aeruginosa ATCC (100 ml). Incubation at 37 C/ 24 hours. Absence of coliforms. B - Blank Plate. C - Water contaminated with S. typhimurium ATCC and E. coli ATCC (100 ml). I Incubation at 37 C/ 24 hours. Presence. D - Inverted Plate. Without yellow halo. E - Inverted Plate. With yellow halo. Sulfite Reducing Clostridia A B C B SPS Agar, Code Description: Sulfite-reducing clostridia form black colonies. A - Blank Plate. B - Water contaminated with C. perfringens ATCC (100 ml). Anaerobic incubation at 37 C/ 24 hours. Presence. C - Water contaminated with C. perfringens ATCC (100 ml). Anaerobic incubation at 37 C/ 72 hours. Presence. B C CCA Coliforms, Chromogenic Agar Code A - Water contaminated with E. coli ATCC and ATCC Incubation at 36 C/ 21 hours. Presence. B - Water contaminated with Salmonella enteritidis ATCC Incubation at 36 C/ 21 hours. Presence. C - Water contaminated with Citrobacter freundii ATCC Incubation at 36 C/ 21 hours. Presence. Pseudomonas aeruginosa A A C Pseudomonas CN Agar (EN ISO 16266) Code Description: P. aeruginosa colonies in this medium are creamy white coloured and with mucosal appearance. A - Blank Plate. B - Bottled Water (250 ml). Incubation at 37 C/ 24 hours. Absence/250 ml. C - Water contaminated with P. aeruginosa ATCC (100 ml). Incubation at 37 C/ 24 hours. Presence. 3

4 m-cp Agar Enumeration of Clostridium perfringens according to Directive 98/83/EC using the membrane method m-cp, Agar Code Culture medium for the enumeration of C. perfringens (spores included) from surface and drinking water. Synonyms: mcp Medium Background: Clostridium perfringens is exclusively of faecal origin and does not grow in the sedimentary aquifers like other members of the sporulated sulfitereducing anaerobes group. Its spores are resistant to heat, disinfection processes and regular sewage treatment. These characteristics mean that several authors have suggested it is an indicator of faecal contamination, of the potential presence of other enteric pathogens, viruses or protozoa. D-Cycloserine L-Cysteine mono-hydrochloride 1-hydrate Yeast Extract Phenolphthalein di-phosphate sol. 0.5% Iron(III) Chloride 6-hydrate sol. 4.5% Indoxyl-β-D-Glucopyranoside 3-hydrate Magnesium Sulfate 7-hydrate Polymixin B Sulfate Bromocresol Purple Saccharose Tryptose Agar ph: 7.6 ± 0.2 Procedure: An appropriate volume of water sample is passed through a sterile 47 mm, 0.45 μm gridded membrane filter, under partial vacuum. The membrane filter is placed on m-cp Agar and incubated anaerobically at 44 ± 1 C for 24 ± 3 hours. The yellow straw-coloured colonies that turn dark pink to violet upon exposure seconds to ammonium hydroxide, are counted as presumptive C. perfringens. Typical colonies of C. perfringens grown on prepared plate m-cp, Agar. C. perfringens colonies grown on m-cp Agar after exposure to ammonium hydroxide vapour. Plates performed in the Microbiology department of Panreac Química SLU. Quality Control Physico-chemical Control Appearance: Satisfactory Colour: Violet ph: 7.6 ± 0.2 Microbiological Test The following results were obtained in the performance of the medium from type cultures after anaerobic incubation at a temperature of 42 ± 2 C and observed after 24 hours: Microorganisms Development Colour of the colony C. perfringens ATCC13124 Good Opaque yellow, turns pink/red after exposure to ammonia vapors C. sporogenes ATCC19404 Good Blue E. coli ATCC Inhibited - Bibliography Council Directive 98/83/EC, L330: (1998) EPA. ICR Microbial Laboratory Manual. Section XI: 1-15 (1996) Real Decreto 140/ Products for Microbiology

5 Microbiology INGREDIENTS Agar, Bacteriological American Type (Ingredient) Solidifying agent used in bacteriological culture media. CAS: EINECS: NC: ph 1.5% before autoclaving ph 1.5% after autoclaving Range of Gelling 1.5% C Melting range 1.5% gel C Gel strength (Nikan s method) 1,5% g/cm 2 MAXIMUM LIMIT OF IMPURITIES Loss on drying at 105 C...20 % Residue on ignition % % g b 6 Agar, Bacteriological European Type (Ingredient) Solidifying agent used in bacteriological culture media. CAS: EINECS: NC: ph 1.5% before autoclaving ph 1.5% after autoclaving Range of Gelling 1.5% C Melting range 1.5% gel C Gel strength (Nikan s method) 1,5% g/cm 2 MAXIMUM LIMIT OF IMPURITIES Loss on drying at 105 C % Residue on ignition... 5 % g b 6 Agar, Purified (Ingredient) For immunodiffusion, electrophoresis and cell culture. CAS: EINECS: NC: : ph Melting range 1.5% gel C Gel strength (Nikan s method) 1,5% g/cm 2 Loss on drying at 105 C % Residue on ignition % g b 6 Agar, Technical (Ingredient) Solidifying agent used in bacteriological culture media. CAS: EINECS: NC: ph 1.5% before autoclaving Gel strength (Nikan s method) 1,5% g/cm 2 MAXIMUM LIMIT OF IMPURITIES Loss on drying at 105 C...20 % Residue on ignition...5 % g b kg G Malt Extract (Ingredient) Nutritional ingredient in media preparation for yeast and moulds. NC: ph sol. 5% Loss on drying at 105 C... 6 % Residue on ignition % g b 6 Meat Extract (Ingredient) Nutrient base in culture media. NC: ph sol. 2% Loss on drying at 105 C...6 % Residue on ignition...16 % Nitrogen, total % g b 6 Peptone, Bacteriological (Ingredient) Product used in bacteriological culture media. EINECS: NC: ph sol. 2% Loss on drying at 105 C... 6 % Residue on ignition...15 % Nitrogen, total % g b 6 Tryptone (Ingredient) Source of nitrogen for culture media. NC: ph sol. 2% Loss on drying at 105 C... 6 % Residue on ignition...15 % Nitrogen, total % g b kg G kg G Yeast Extract (Ingredient) Nutrient base in culture media. NC: ph sol. 2% Dry matter % Nitrogen, total % g b 6 Casein Peptone (Ingredient) Nutritional ingredient to prepare culture media. NC: ph sol. 2% Loss on drying at 105 C... 6% Residue on ignition...15 % Nitrogen, total % g b kg G 5

6 DEHYDRATED CULTURE MEDIA AND SUPPLEMENTS (See also Prepared Media) Acetamide Broth (EN ISO 16266) Culture medium for the confirmation of Pseudomona aeruginosa. according to EN ISO H: H351 P: P201 P202 P281 P308+P313 P405 P501 Potassium di-hydrogen Phosphate Magnesium Sulfate Acetamide Sodium Chloride ph: 7.0 ± g b 6 Antibiotic Medium nº 1 (USP) Culture medium for antibiotic assay testing. Meat Extract Yeast Extract D(+)-Glucose Casein Peptone Gelatin Peptone ph: 6.6 ± g b 6 Baird-Parker Agar Base Culture medium for the determination and the enumeration of Staphylococci. Meat Extract Yeast extract Glycine Lithium Chloride Digest Pancreatic of Casein Sodium Pyruvate Agar ph: 6.8 ± g b 6 Bile Esculin Azide Agar (ISO :2000) Culture medium for presumptive identification of Enterococci. according to ISO : H: H302 H412 P: P264 P270 P273 P301+P312 P330 P501 Ox Bile Esculin Sodium Azide Yeast extract Iron(III) Citrate Peptone Sodium Chloride Tryptone ph: 7.1 ± g b 6 Brain Heart Infusion (BHI) Culture medium for fastidious microorganisms. Calf Brain Infusion Meat Heart Infusion D(+)-Glucose Gelatin Peptone Sodium Chloride di-sodium Hydrogen Phosphate g b 6 Brain Heart Infusion Agar (BHI) Culture medium for fastidious microorganisms. Calf Brain Infusion Meat Heart Infusion D(+)-Glucose Mixture of Peptones di-potassium Hydrogen Phosphate Sodium Chloride g b 6 Brilliant Green Agar Culture medium for the isolation of Salmonella. Brilliant Green Yeast Extract Lactose Peptones (meat and casein) Phenol Red Saccharose Sodium Chloride Agar ph: 6.9 ± g b 6 Brilliant Green Bile 2% Broth (ISO 4831, ISO 4832) Culture medium for the detection and enumeration of Coliforms. Ox Bile, dehydrated Brilliant Green Lactose Gelatin Peptone g b 6 6 Products for Microbiology

7 Microbiology Buffered Peptone Water (ISO 6579, ISO 22964, ISO 6887, DIN 10181, 10160) Diluent agent to homogenize samples in foodstuffs. Digest Pancreatic of Casein Potassium di-hydrogen Phosphate Sodium Chloride di-sodium Hydrogen Phosphate (*) (*) It is equivalent to di-sodium Hydrogen Phosphate 12-hydrate g b 6 Buffered Sodium Chloride-Peptone solution (Ph. Eur.) Diluent agent for the homogenization of samples. NC: Digest Pancreatic of Casein Potassium di-hydrogen Phosphate Sodium Chloride di-sodium Hydrogen Phosphate 2-hydrate g b kg G Candida Chromogenic Agar Chromogenic medium for the isolation and quick identification. of Candida spp. Dextrose Chromogenic mixture Peptone Chloramphenicol Bacteriological Agar ph: 6.1 ± g b 6 Cefixime Selective Enrichment (Supplement) Supplement for E. coli O157:H7 Chromogenic Agar Base. fh: H301 H319 H315 P: P264 P270 P280 P301+P310 P302+P352 P501 P305+P351+P338 P321 P330 P332+P313 P337+P313 P362 P405 Composition (mg/1 vial): Potassium Tellurite Cefixime vials v 6 Cetrimide Agar (Ph. Eur.) Culture medium for the enumeration of Pseudomonas aeruginosa. Cetrimide Magnesium Chloride Pancreatic Digest of Gelatine Potassium Sulfate Agar g b 6 Chapman TTC (Tergitol 7) Agar (ISO :2000) Culture medium for the detection and enumeration of Coliforms. Bromothymol Blue Meat Extract Yeast Extract Lactose Meat Peptone Sodium Heptadecyl Sulfate g b 6 Chapman USP Medium (see Mannitol Salt Agar) Chromogenic E. coli Agar Culture medium for the simultaneous detection of total Coliform and E. coli. Chromogenic mixture Bacteriological Peptone Sodium Chloride Sodium Pyruvate Sorbitol Phosphate Buffer Tergitol Tryptophan Agar ph: 6.8 ± g b g b 6 Chromogenic Salmonella Agar Culture medium for isolation of Salmonella. Chromogenic mixture Meat Extract Casein Peptone Sodium Citrate Agar g b 6 CLED Medium Culture medium for the enumeration and presumptive identification of microorganisms from urine. Bromothymol Blue L-Cystine Meat Extract Lactose Casein Peptone Gelatin Peptone ph: 7.3 ± g b 6 7

8 DNase Agar Culture medium for the differentiation of Staphylococcus species and Serratia marcescens, based on their production of deoxyribonuclease. Deoxyribonucleic Acid Casein Peptone Soy Peptone Sodium Chloride ph: 7.3 ± g b 6 EC Medium Culture medium for the cultivation of Coliform bacterias. Tryptose Lactose Potassium di-hydrogen Phosphate di-potassium Hydrogen Phosphate Bile Salts nº Sodium Chloride ph: 6.9 ± g b 6 E. coli O157:H7 Chromogenic Agar Base Selective and differential medium for detection of E. coli O157:H7. Mixture of Peptones Chromogenic mixture Bacteriological Agar g b 6 Egg Yolk Emulsion (Supplement) Additive for culture media for detection of lecitinase activity ml a 6 Egg Yolk Tellurite Emulsion (Supplement) Additive for culture media for detection of lecitinase activity ml a ml a 6 Enterobacter Sakazakii Chromogenic Agar (ISO 22964) Chromogenic medium for the isolation of presumptive Enterobacter Sakazakii in infant milk products. Digest Pancreatic of Casein Sodium Chloride Yeast Extract Sodium Deoxycholate α-d-glucopyranoside Crystal Violet Bacteriological Agar g b 6 Eosin Methylene Blue Agar (EMB) Culture medium for the isolation of Gram-negative enteric bacteria. Eosin Yellowish Methylene Blue Lactose Bacteriological Peptone di-potassium Hydrogen Phosphate Saccharose Agar g b 6 FDA M169 (see TSC Agar Base) Fraser Listeria Broth Base (ISO :1996) Enrichment medium for detection and enumeration of Listeria monocytogenes. Esculin Yeast extract Meat Extract Lithium Chloride Potassium di-hydrogen Phosphate Proteose Peptone Sodium Chloride di-sodium Phosphate Tryptone g b 6 Fraser Listeria Selective Enrichment (Supplement) Supplement used for the determination of Listeria monocytogenes. gh: H302 H315 H319 H335 P: P261 P305+P351+P338 Composition (mg/vial): Ammonium Iron(III) Citrate Nalidixate Sodium Acryflavine vials v 6 Fraser 1/2 Listeria Selective Enrichment (Supplement) Additive used for the enrichment of Listeria monocytogenes. g H: H302 H315 H319 H335 P: P261 P305+P351+P338 Composition (mg/vial): Ammonium Iron(III) Citrate Nalidixate Sodium Acryflavine vials v 6 8 Products for Microbiology

9 Microbiology Glucose Chloramphenicol Agar Culture medium for the count and the isolation of fungi. D(+)-Glucose Chloramphenicol Yeast Extract ph: 6.6 ± g b 6 Hektoen Enteric Agar (ISO 21567) Culture medium for the isolation and differentiation of Salmonella and Shigella. Ammonium Iron(III) Citrate Bromothymol Blue Yeast extract Fuchsin Acid Lactose Meat Peptone Saccharose Bile Salts D(-)-Salicin Sodium Chloride Sodium Thiosulfate Agar ph: 7.5 ± g b 6 Kanamycin Esculin Azide Agar (CeNAN) Medium for the enumeration and confirmation of Enterococci. gh: H302 H412 P: P264 P270 P273 P301+P312 P330 P501 Kanamycin Sulfate Esculin Sodium Azide Ammonium Iron(III) Citrate Yeast Extract Sodium Chloride di-sodium Hydrogen Citrate Tryptone g b 6 KF Streptococcus Agar Culture medium for the isolation of enterococci in water. gh: H302 P: P264 P270 P301+P312 P330 P501 Yeast Extract Lactose Maltose Mixture of Peptones Sodium Azide Sodium Chloride Sodium Glycerophosphate Agar g b 6 King B Medium Medium for the differentiation of Pseudomonas based in the production of Fluorescein. Magnesium Sulfate Polypeptone di-potassium Hydrogen Phosphate g b 6 Kligler Iron Agar Medium for the identification of Gram-negative enteric bacilli. Ammonium Iron(III) Citrate D(+)-Glucose Lactose Mixture of Peptones Phenol Red Sodium Chloride Sodium Thiosulfate g b 6 Lactosed Broth Medium for the detection of Coliforms, specially E. coli. Lactose Meat Extract Gelatin Peptone ph: 6.9 ± g b 6 Lauryl Sulfate Chromogenic Broth Enrichment medium for the simultaneous detection of total Coliforms and E. coli. Tryptose Sodium Chloride di-potassium Phosphate mono-potassium Phosphate Sorbitol Tryptophan Chromogenic-Fluorogenic Mix Lauryl Sodium Sulfate ph: 6.8 ± g b 6 Legionella Agar (BCYEx) (see Prepared Media: BCYEx Agar) 9

10 Letheen Agar (modified) Culture medium for the determination of the antimicrobial activity of quaternary ammonium compounds. Meat Extract Yeast Extract Lecithin Casein Peptone Meat Peptone Dextrose Sodium Chloride Sodium Bisulfite Polysorbate Bacteriological Agar g b 6 Letheen Broth (modified) Culture medium for the determination of the antimicrobial activity of quaternary ammonium compounds. Meat Extract Yeast Extract Lecithin Casein Peptone Meat Peptone Dextrose Sodium Chloride Sodium Bisulfite Polysorbate g b 6 Lipase C Selective Enrichment (Supplement) Selective supplement for the isolation of Listeria. Formula per vial: Lipase C Substrate mg vials v 6 Listeria Chromogenic Agar (ISO :2004) Selective medium for the detection and enumeration of Listeria monocytogenes. Meat Peptone Lithium Chloride Yeast extract Tryptone Sodium Chloride di-sodium Hydrogen Phosphate anhydrous Dextrose Sodium Pyruvate Magnesium Glycerophosphate Magnesium Sulfate X-Glucoside Bacteriological Agar g b 6 Listeria Selective Enrichment Chromogenic (Supplement) Supplement used for the determination of Listeria monocytogenes. Formula per vial: Polymixin B Sulfate UI Ceftazidime...10 mg Nalidixic acid...10 mg Cycloheximide mg vials v 6 Luria Broth Base Culture medium for the development of Escherichia coli. Casein Peptone Yeast Extract Sodium Chloride g b 6 Lysine Iron Agar Medium for the differentiation of Salmonella and Arizona. Ammonium Iron(III) Citrate L-Lysine Yeast Extract D(+)-Glucose Gelatin Peptone Bromocresol Purple Sodium Thiosulfate Agar ph: 6.7 ± g b 6 MacConkey Agar (Ph. Eur., ISO 21567) Culture medium for Coliforms. Lactose Peptones (meat and casein) Bile Salts Gelatin Peptone Neutral Red Sodium Chloride Crystal Violet Agar ph: 7.1 ± g b 6 MacConkey Broth (Ph. Eur.) Culture medium for Coliforms. Ox Bile Lactose Gelatin Peptone Bromocresol Purple ph: 7.3 ± g b 6 10 Products for Microbiology

11 Microbiology Malt Extract Agar Culture medium for the isolation and enumeration of yeast and fungi. Malt Extract Dextrin Glycerol Gelatin Peptone ph: 4.7 ± g b 6 Mannitol Salt Agar (Ph. Eur.) Medium for cultivation and enumeration of Staphylococci. Sodium Chloride D(-)-Mannitol Meat Extract Digest Pancreatic of Casein Peptic Digest of Animal Tissue Phenol Red g b 6 Mannitol-Salt-Phenol Red Agar (see Mannitol Salt Agar) Marine Agar Medium for the cultivation of heterotrophic marine bacteria. Boric Acid Ammonium Nitrate Calcium Chloride Strontium Chloride Yeast Extract Iron Citrate Magnesium Chloride Peptone Potassium Bromide Potassium Chloride Sodium Chloride Sodium Fluoride Sodium Hydrogen Carbonate di-sodium Hydrogen Phosphate Sodium Silicate Sodium Sulfate ph: 7.6 ± g b 6 Marine Broth Medium for the cultivation of heterotrophic marine bacteria. Boric Acid Ammonium Nitrate Calcium Chloride Strontium Chloride Yeast Extract Iron Citrate Magnesium Chloride Bacteriological Peptone Potassium Bromide Potassium Chloride Sodium Chloride Sodium Fluoride Sodium Hydrogen Carbonate di-sodium Hydrogen Phosphate Sodium Silicate Sodium Sulfate ph: 7.6 ± g b 6 Maximum Recovery Diluent (MRD) (ISO 6887) General diluent for a wide variety of microorganisms. Casein Peptone Sodium Chloride g b 6 Medium A ((see Tryptone Soy Broth (TSB)) Medium B (see Tryptone Soy Agar (TSA)) Medium C (see Sabouraud Glucose Agar + Chloramphenicol) Medium D (see Lactosed Broth) Medium G (see MacConkey Broth) Medium H (see MacConkey Agar) Medium K (see XLD Medium) Medium L (see Brilliant Green Agar) Medium M (see Triple Sugar Iron Agar) Medium N (see Cetrimide Agar) Medium O (see Baird-Parker Agar Base) Medium S (see R2A Agar) 11

12 Minerals (modified) Glutamated Broth (MMGB) (ISO ) Broth used for presumptive identification of coliforms in water. Sodium L-Glutamate Lactose Sodium Formate L-Cystine L-Aspartic Acid L-Arginine Thiamine Nicotinic Acid Pantothenic Acid Magnesium Sulfate 7-hydrate Ammonium Iron(III) Citrate Calcium Chloride 2-hydrate di-potassium Hydrogen Phosphate Bromocresol Purple ph: 6.7 ± g b 6 MRS Agar Medium for the cultivation of Lactobacillus species. di-ammonium Hydrogen Citrate Meat Extract Yeast Extract D(+)-Glucose Magnesium Sulfate Manganese(II) Sulfate Bacteriological Peptone di-potassium Hydrogen Phosphate Sodium Acetate Tween Agar ph: 6.2 ± g b 6 MRS Broth Medium for the cultivation of Lactobacillus species. di-ammonium Hydrogen Citrate Meat Extract Yeast Extract D(+)-Glucose Magnesium Sulfate Manganese(II) Sulfate Bacteriological Peptone di-potassium Hydrogen Phosphate Sodium Acetate Tween ph: 6.2 ± g b 6 Mueller-Hinton Agar Medium for sensitivity test for diverse antibiotics and sulfonamides. Starch Meat Infusion Casein Peptone Hydrolysate Agar g b 6 Mueller-Hinton Broth Medium for sensitivity test in broth for diverse antibiotics. Starch Meat Infusion Casein Peptone Hydrolysate g b 6 Nutrient Agar (ISO 6579, ISO 10273, ISO 19250) Medium for enumeration of organisms in water. Meat Extract Gelatin Peptone ph: 6.8 ± g b 6 Nutrient Agar (EN ISO 16266) Medium for the differential subculture of Pseudomonas aeruginosa according to EN ISO Peptone Meat Extract Yeast Extract Sodium Chloride g b 6 Nutrient Broth Medium for the cultivation of non fastidious microorganisms. ESPECIFICACIONES: Composición (g/l): Meat Extract Gelatin Peptone ph: 6.8 ± g b 6 OGYE Agar Base Medium for the enumeration and cultivation of yeasts and fungi. Yeast extract D(+)-Glucose ph: 6.5 ± 0.2 Order code Package Unid. caja estándar g b 6 Orange Serum Agar Culture medium for the isolation of acid tolerant microorganisms in fruit juices. Casein Peptone Yeast Extract Orange Extract Dextrose Potassium di-hydrogen Phosphate ph: 5.5 ± g b 6 12 Products for Microbiology

13 Microbiology Oxford Listeria Selective Enrichment (Supplement) Additive used for Listeria monocytogenes detection. UN: 2811 IMDG: 6.1/I ADR: 6.1/I IATA: 6.1/I PAX: 606 CAO: 607 8f H: H360D H300 H341 P: P20 P202 P264 P270 P281 P501 P301 +P310 P308+P313 P321 P330 P405 Composition (mg/vial): Cycloheximide Colystine Sulfate Acryflavine Cefotetan Phosphomycine vials v 6 Oxidase Sticks Test sticks used for the easy and rapid detection of the cytochrome-oxidase enzyme. 50 plastic sticks. Composition (per stick): Tetramethyl-p-phenylenadiamine hydrochloride...8% (p/v) sticks v 6 PALCAM Listeria Agar Base Medium for the selective isolation, cultivation and differentiation of Listeria monocytogenes. 7H: H319 H315 P: P264 P280 P302+P352 P305+P351+P338 P321 P501 P332+P313 P337+P313 P362 Composition (g/l ): Columbia Agar Base Yeast extract Dextrose Esculin Ammonium Iron(III) Citrate Mannitol Phenol Red Lithium Chloride g b 6 PCA (see Standard Methods Agar (APHA). Prepared Media: Plate Count Agar (PCA)) Peptone of Casein-Glucose-Yeast Extract Agar (see Standard Methods Agar (APHA). Prepared Media: Plate Count Agar (PCA)) Peptone Water Diluent agent for the homogenization of samples. Tryptone Sodium Chloride g b 6 Perfringens according to Angelotti Selective Agar (see SPS Agar (Selective Agar according to Angelotti)) Potassium Tellurite solution 3,5% (Supplement) Additive selective for culture media. 7H: H302 P: P264 P270 P301+P312 P330 P ml a 6 Potato Glucose Agar (Ph. Eur.) Medium for the culture and enumeration of yeast and fungi. D(+)-Glucose Potatoes Infusion (200 g) ph: 5.6 ± g b 6 Pseudomonas CN Agar Base (EN ISO 16266) Medium for the enumeration of Pseudomonas aeruginosa. Cetrimide Nalidixic Acid Magnesium Chloride Casein Peptone Hydrolysate Gelatin Peptone Potassium Sulfate Agar ph: 7.1 ± g b 6 R2A Agar (Ph. Eur.) Medium for the enumeration of heterotrophic bacteria in water according to Ph. Eur. Proteose Peptone Casein Hydrolyzed Yeast Extract Dextrose Starch Sodium Pyruvate di-potassium Hydrogen Phosphate Magnesium Sulfate g b 6 Rappaport-Vassiliadis (RVS) Broth (ISO 6579, ISO 19250) Enrichment broth for Salmonella. Magnesium Chloride anhydrous * Soy Peptone Potassium di-hydrogen Phosphate di-potassium Hydrogen Phosphate Sodium Chloride Malachite Green ph: 5.2 ± 0.2 *It is equivalent to Magnesium Chloride 7-hydrate g b 6 13

14 Rose Bengal Chloramphenicol Agar Culture medium for the enumeration and isolation of yeast and fungi. Rose Bengal Chloramphenicol D(+)-Glucose Magnesium Sulfate Bacteriological Peptone Potassium di-hydrogen Phosphate g b 6 RPF Selective Enrichment (ISO-FDIS ) (Supplement) Additive used for the detection of coagulase positive Staphylococcus. Composition (per vial): Rabbit Plasma ml Bovine Fibrinogen mg Trypsin Inhibitor mg Potassium Tellurite mg vials v 6 Sabouraud Agar (see Sabouraud Glucose Agar) Sabouraud+Chloramphenicol Agar (see Sabouraud Glucose Agar+Chloramphenicol) Sabouraud Glucose Agar (Ph. Eur.) Medium for the cultivation and enumeration of fungi and yeasts. D(+)-Glucose Mixture of Peptic Digest of Animal Tissue and Pancreatic Digest of Casein (1:1) ph: 5.6 ± g b 6 Sabouraud Glucose Agar+Chloramphenicol (Ph. Eur.) Medium for the cultivation and enumeration of fungi and yeasts. D(+)-Glucose Chloramphenicol Mixture of Peptones ph: 5.6 ± g b 6 Glucose Sabouraud Broth (Ph. Eur.) Culture medium for antibiotic effectiveness test and in fungi and yeast cultures. D(+)-Glucose Mixture of Peptic Digest of Animal Tissue and Pancreatic Digest of Casein (1:1) ph: 5.6 ± g b 6 Salmonella Shigella Agar Medium for the isolation of Shigella and Salmonella. Meat Extract Iron(III) Citrate Lactose Peptones Neutral Red Bile Salts tri-sodium Citrate Sodium Thiosulfate Brilliant Green Agar g b 6 Selenite Cystine Broth Medium for the enrichment of Salmonella. UN: 3077 IMDG: 9/III ADR: 9/III IATA: 9/III PAX: 911 CAO: H: H332 H302 H373 H411 P: P260 P261 P264 P270 P271 P273 P301+P312 P304+P340 P312 P314 P330 P391 P501 Sodium Hydrogen Selenite L(-)-Cystine Lactose Mixture of Peptones tri-sodium Phosphate g b 6 Slanetz Bartley Medium (ISO :2000) Culture medium for the enumeration of Enterococci. 7 H: H302 P: P264 P270 P301+P312 P330 P501 Yeast Extract D(+)-Glucose Sodium Azide di-potassium Hydrogen Phosphate ,3,5-Triphenyl-2H-Tetrazolium Chloride Tryptose Agar g b 6 SPS Agar (Selective Agar according to Angelotti) Culture medium for the detection and enumeration of sulfite-reducing clostridia. Sodium Sulfite Polymixin B Sulfate Sodium Sulfadiazine Yeast Extract Iron(III) Citrate Casein Peptone Agar g b 6 14 Products for Microbiology

15 Microbiology Standard Methods Agar (APHA) (ISO 4833:2003) Culture medium for the enumeration of microorganisms. Yeast Extract D(+)-Glucose (Anhydrous) Enzym Digest of Casein Bacteriological Agar g b 6 Staphylococcus according to Baird-Parker Selective Agar (see Baird-Parker Agar Base) Sterility Test Broth (see Thioglycollate Liquid Medium) TBX Agar (ISO ,3:2000) Selective culture medium for the determination and the enumeration of E. coli according to ISO :2000 Casein Peptone Bile Salts X-B-D-Glucuronide g b 6 TCBS Cholera Medium Medium for the cultivation and isolation of Vibrio cholerae and Vibrio parahaemolyticus. NC: Bromothymol Blue Thymol Blue Dried Bile Yeast Extract Iron(III) Citrate Meat Peptone Casein Peptone Saccharose tri-sodium Citrate Sodium Chloride Sodium Cholate Sodium Thiosulfate Agar ph: 8.6 ± g b 6 Tergitol 7 Agar (see Chapman TTC (Tergitol 7) Agar) Tetrathionate Broth Base acc.to Muller-Kauffmann Broth base for the enrichment of Salmonella. Ox Bile Calcium Carbonate Meat Extract Yeast Extract Meat Peptone Sodium Chloride Sodium Thiosulfate ph: 7.6 ± g b 6 Thioglycollate Liquid Medium (Ph. Eur., USP, ISO 7937) Medium for the cultivation of aerobic and anaerobic organisms in the performance of sterility tests. Sodium Thioglycollate L-Cystine Yeast Extract D(+)-Glucose Enzym Digest of Casein Resazurin Sodium Chloride Agar ph: 7.1 ± g b 6 Triple Sugar Iron Agar Medium for the differentiation of Enterobacteriaceae. Ammonium Iron(III) Citrate D(+)-Glucose Meat Extract Yeast Extract Lactose Saccharose Peptone Mixture (Meat/Casein) Phenol Red Sodium Chloride Sodium Thiosulfate Agar g b 6 Tryptone, Bile, X-Glucuronide Agar (see TBX Agar) Tryptone Agar with bile salts (see Prepared Media: TBA Agar) Tryptone Soy Agar (TSA) (Ph. Eur.) Medium for the cultivation of a wide variety of microorganisms. Papaic Digest of Soya Digest Pancreatic of Casein ph: 7.3 ± g b kg G Tryptone Soy Broth (TSB) (Ph. Eur.) Medium for the culture of a wide variety of microorganisms. Papaic Digest of Soya D(+)-Glucose Digest Pancreatic of Casein di-potassium Hydrogen Phosphate ph: 7.3 ± g b 6 15

16 Tryptone Soy+Tween+Lecithin Agar (see Prepared Media: TSA-Tween-Lecithin-Agar) Tryptone Water (see Peptone Water) Tryptone Yeast Extract Agar (ISO 6222:1999) Medium for the enumeration of microorganisms according to ISO 6222:1999 Yeast Extract Tryptone g b 6 Tryptose-Sulfite-Cycloserine Agar (see TSC Agar Base) TSA (see Tryptone Soy Agar (TSA)) TSA-Polysorbate-Lecithin Agar (see Prepared Media: TSA-Tween-Lecithin-Agar) TSB (see Tryptone Soy Broth (TSB)) TSC Agar Base (ISO 7937) Culture medium for the detection and enumeration of Clostridium perfringens and other anaerobics in water, foods and other materials. Yeast Extract Iron(III) Citrate Soy Peptone Sodium Disulfite Tryptose ph: 7.6 ± g b 6 TSN Agar Culture medium for the enumeration of Clostridium perfringens. Neomycin Sulfate Sodium Sulfite Yeast Extract Iron(III) Citrate Casein Peptone Polymixin B Sulfate Agar g b 6 Violet Red Bile Glucose Agar (VRBG) (Ph. Eur. ) (ISO 21528) Culture medium for the enumeration of Enterobacteriaceae. Bile Salts Mixture Crystal Violet Neutral Red D(+)-Glucose Yeast Extract Pancreatic Digest of Gelatine g b 6 Violet Red Bile Lactose Agar (VRBL) (ISO 4832) Culture medium for the detection and enumeration of Coliforms. Bile Salts nº Crystal Violet Neutral Red Lactose Yeast Extract Gelatin Peptone g b 6 VRBG Agar (see Violet Red Bile Glucose Agar (VRBG)) VRBL Agar (see Violet Red Bile Lactose Agar (VRBL)) WL Nutrient Agar Culture medium for the determination of microbian flora in the brewing processes and other fermentation industries. Calcium Chloride Yeast Extract D(+)-Glucose Iron(III) Chloride Magnesium Sulfate Manganese(II) Sulfate Potassium Chloride Potassium di-hydrogen Phosphate Tryptone Bromocresol Green ph: 5.5 ± g b 6 16 Products for Microbiology

17 Microbiology XLD Agar (ISO 6579, ISO 19250, ISO 21567) Culture medium for the isolation of Salmonella and Shigella according to ISO 6579:2002 Ammonium Iron(III) Citrate Yeast Extract Lactose L-Lysine Phenol Red Saccharose Sodium Deoxycholate Sodium Thiosulfate D(+)-Xylose Agar g b 6 XLD Medium (Ph. Eur.) Culture medium for the isolation of Salmonella and Shigella. Ammonium Iron(III) Citrate Yeast Extract Lactose L-Lysine Phenol Red Saccharose Sodium Deoxycholate Sodium Thiosulfate D(+)-Xylose Agar g b 6 PREPARED MEDIA PREPARED PLATES FOR WATER ANALYSIS THROUGH MEMBRANE FILTRATION CCA Coliforms, Chromogenic Agar (Prepared Plate (Ø 55 mm)) Selective medium for the detection of total coliforms and E. coli. Peptone Monosodium Phosphate di-sodium Phosphate Sodium Pyruvate L-Tryptophan Agar Sorbitol ,3,5-Triphenyl-2H-Tetrazolium Chloride Cefsulodin Vancomycin Chromogenic b GLU Susbstrate Chromogenic Salmon GAL Substrate ph: 6.8 ± plates v 6 m-cp Agar (Prepared Plate (Ø 55 mm)) Culture medium for the enumeration of C. perfringens. D-Cycloserine...0,4 L-Cysteine mono-hydrochloride 1-hydrate...1,0 Yeast Extract... 20,0 Phenolphthalein di-phosphate sol. 0,5%... 20,0 Iron(III) Chloride 6-hydrate sol. 4,5%...2,0 3-Indoxyl-B-D-Glucopyranoside 3-hydrate... 0,06 Magnesium Sulfate 7-hydrate...0,1 Polymixin B Sulfate...0,025 Bromocresol Purple... 0,04 Saccharose...5,0 Tryptose... 30,0 Agar... 15,0 ph: 7.6 ± plates v 6 Nutrient Agar (ISO 6579, ISO 10273, ISO 19250) (Prepared Plate (Ø 55mm)) Medium for enumeration of organisms. Meat Extract Meat Peptone Agar plates v 6 Pseudomonas CN (EN ISO 16266) (Prepared Plate (Ø 55 mm)) Medium for the enumeration of Pseudomonas aeruginosa. Cetrimide Nalidixic Acid Glycerol Magnesium Chloride Casein Peptone Gelatin Peptone Potassium Sulfate Agar ph: 7.2± plates v 6 R2A Agar (Ph. Eur.) (Prepared Plate (Ø 55 mm)) Medium for the enumeration of heterotrophic bacteria in water according to Ph. Eur. E Proteose Peptone Casein Peptone Yeast Extract Dextrose Starch soluble Sodium Pyruvate di-potassium Hydrogen Phosphate Magnesium Sulfate plates v 6 17

18 Slanetz Bartley Medium (ISO :2000) (Prepared Plate (Ø 55 mm)) Medium for the enumeration of Enterococci. 7H: H302 P: P264 P270 P301+P312 P330 P501 Yeast Extract D(+)-Glucose di-potassium Hydrogen Phosphate Sodium Azide ,3,5-Triphenyl-2H-Tetrazolium Chloride Tryptose Agar plates v SPS Agar (Prepared Plate (Ø 55 mm)) Medium for the enumeration of sulfite-reducing clostridia. Sodium Sulfite Polymixin B Sulfate Sodium Sulfadiazine Yeast Extract Iron(III) Citrate Casein Peptone Agar plates v TBA Agar (ISO :2000) (Prepared Plate (Ø 55 mm)) Medium for the determination and the enumeration of E. coli according to ISO :2000 Tryptone Peptone Bile Salts plates v Tergitol 7 Agar (Chapman TTC modified) (ISO :2000) (Prepared Plate (Ø 55 mm)) Medium for the enumeration of total and faecal Coliforms. Bromothymol Blue Yeast Extract Meat Extract Lactose Peptone Sodium Heptadecyl Sulfate ,3,5-Triphenyl-2H-Tetrazolium Chloride Agar ph: 7.5 ± plates v Tryptone Yeast Extract Agar (ISO 6222:1999) (Prepared Plate (Ø 55 mm)) Medium for the enumeration of microorganism in water according to ISO 6222:1999. Yeast Extract Tryptone plates v TSC, Agar (ISO 7937) (Prepared Plate (Ø 55 mm)) Culture medium for the detection and enumeration of Clostridium perfringens and other anaerobics in water, foods and other materials. Yeast Extract Iron(III) Citrate Soy Peptone Sodium Disulfite Tryptose Cycloserine Agar ph: 7.6 ± plates v CONTACT PLATES FOR HYGIENE SURFACE CONTROL Baird-Parker Agar (ISO 6888) (Contact Plate) Medium for the determination and the enumeration of Staphylococci. Egg Yolk Emulsion Meat Extract Yeast Extract Glycine Lithium Chloride Potassium Tellurite Sodium Pyruvate Tryptone Agar ph: 6.9 ± plates v PCA Agar (ISO 4833:2003) (Contact Plate) Medium for the enumeration of microorganisms. Yeast Extract D(+)-Glucose Tryptone Agar plates v Rose Bengal Chloramphenicol Agar (Contact Plate) Medium for the enumeration and isolation of yeast and fungi. Rose Bengal Chloramphenicol D(+)-Glucose Magnesium Sulfate Peptone Potassium di-hydrogen Phosphate Agar plates v Sabouraud Glucose Agar+Chloramphenicol (Ph. Eur.) (Contact Plate) Medium for the cultivation and enumeration of fungi and yeasts. D(+)-Glucose Chloramphenicol Mixture of Peptones Agar ph: 5.6 ± plates v 18 Products for Microbiology

19 Microbiology Tryptone Soy Agar (TSA) (Ph. Eur.) (Contact Plate) Medium for the cultivation of a wide variety of microorganisms. Soy Peptone Casein Peptone ph: 7.3 ± plates v TSA-Tween-Lecithin-Agar (Ph. Eur.) (Contact Plate) Medium for detection and enumeration of a wide range of microorganisms and ability to neutralize the antibacterial activity of some bactericides. Polisorbate Lecithin Histidine Casein Peptone Soy Peptone Sodium Thiosulfate ph: 7.3 ± plates v Violet Red Bile Glucose Agar (VRBG) (Ph. Eur.) (Contact Plate) Medium for the enumeration of Enterobacteriaceae. Bile Salts Mixture Crystal Violet Neutral Red D(+)-Glucose Yeast Extract Gelatin Peptone Agar plates v PREPARED PLATES (Ø 90 mm) Baird-Parker Agar (ISO 6888) (Prepared Plate (Ø 90 mm)) Medium for the determination and the enumeration of Staphylococci. Egg Yolk Emulsion Meat Extract Yeast Extract Glycine Lithium Chloride Potassium Tellurite Sodium Pyruvate Tryptone Agar ph: 6.9 ± plates v BCYEx Agar (ISO 11731:1998) (Prepared Plate (Ø 90 mm)) Culture medium for the cultivation and isolation of Legionella. ACES Charcoal Activated L-Cysteine Chloride Yeast Extract Ferric Pyrophosphate α-ketoglutarate Potassium Hydroxide Agar ph: 6.9 ± plates v Bile Esculin Azide Agar (ISO :2000) (Prepared Plate (Ø 90 mm)) Medium for the presumptive identification of Enterococci according to ISO : H: H302 H412 P: P264 P270 P273 P301+P312 P330 P501 Ox Bile Esculin Sodium Azide Yeast Extract Iron(III) Citrate Peptone Tryptone ph: 7.1 ± plates v Enterobacter Sakazakii Chromogenic Agar (ISO 22964) (Prepared Plate (Ø 90 mm)) Chromogenic medium for the isolation of presumptive Enterobacter Sakazakii in infant milk products. Digest Pancreatic of Casein Yeast Extract Sodium Deoxycholate α-d-glucopyranoside Crystal Violet Bacteriological Agar plates v 6 19

20 Legionella Selective Agar (ISO 11731:1998) (Prepared Plate (Ø 90 mm)) Medium for the cultivation and isolation of Legionella species. ACES Buffer Charcoal Activated Cycloheximide L-Cysteine Chloride Yeast Extract Glycine Ferric Pyrophosphate α-ketoglutarate Polymixin B Sulfate UI Potassium Hydroxide Vancomycin Agar ph: 6.9 ± plates v 6 Listeria Chromogenic Agar (ISO :2004) (Prepared Plate (Ø 90 mm)) Selective medium for the detection and enumeration of Listeria monocytogenes. Meat Peptone Lithium Chloride Yeast Extract Tryptone di-sodium Hydrogen Phosphate anhydrous Dextrose Sodium Pyruvate Magnesium Glycerophosphate Magnesium Sulfate X-Glucoside Lipase C Sustrat Cycloheximide Ceftazimide Nalidixic Acid Polymixine B IU Bacteriological Agar plates v 6 Nutrient Agar (ISO 6579, ISO 10273, ISO 19250) (Prepared Plate (Ø 90 mm)) Medium for enumeration of organisms in water. Meat Extract Meat Peptone Agar plates v Plate Count Agar (ISO 4833:2003) (Prepared Plate (Ø 90 mm)) Culture medium for the enumeration of microorganisms. Yeast Extract D(+)-Glucose Casein Peptone plates v Rose Bengal Chloramphenicol Agar (Ph. Eur.) (Prepared Plate (Ø 90 mm)) Culture medium for the enumeration and isolation of yeast and fungi. Rose Bengal Chloramphenicol D(+)-Glucose Magnesium Sulfate Peptone Potassium di-hydrogen Phosphate Agar plates v Sabouraud Glucose Agar (Ph. Eur.) (Prepared Plate (Ø 90 mm)) Medium for the cultivation and enumeration of fungi and yeasts. D(+)-Glucose Casein Peptone Meat Peptone Agar ph: 5.6 ± plates v Sabouraud Glucose Agar+Chloramphenicol (Ph. Eur.) (Prepared Plate (Ø 90 mm)) Medium for the cultivation and enumeration of fungi and yeasts. D(+)-Glucose Chloramphenicol Casein Peptone Meat Peptone ph: 5.6 ± plates v Sabouraud Glucose Agar+Chloramphenicol (Ph. Eur.) (irradiated) (Prepared Plate (Ø 90 mm)) Medium for the cultivation and enumeration of fungi and yeasts. D(+)-Glucose Chloramphenicol Casein Peptone Meat Peptone ph: 5.6 ± plates v Salmonella Chromogenic Media (Prepared Plate (Ø 90 mm)) Selective medium for the isolation of Salmonella in foods and other samples. Chromogenic mixture IPTG Casein Peptone Meat Extract Ferric Ammonium Citrate Sodium Citrate Sodium Deoxycholate Bacteriological Agar plates v 6 20 Products for Microbiology

21 Microbiology TBX Agar (ISO ,3:2000) (Prepared Plate (Ø 90 mm)) Medium for the determination and the enumeration of E. coli according to ISO :2000 Casein Peptone X-B-D-Glucuronide Bile Salts Bacteriological Agar plates v 6 Tryptone Soy Agar (TSA) (Ph. Eur.) (Prepared Plate (Ø 90 mm)) Medium for the cultivation of a wide variety of microorganisms. Soy Peptone Casein Peptone ph: 7.3 ± plates v TSA-Tween-Lecithin-Agar (Ph. Eur.) (Prepared Plate (Ø 90 mm)) Medium for detection and enumeration of a wide range of microorganisms and ability to neutralize the antibacterial activity of some bactericides. Polisorbate Lecithin Histidine Casein Peptone Soy Peptone Sodium Thiosulfate ph: 7.3 ± plates v Violet Red Bile Glucose Agar (VRBG) (Ph. Eur.) (Prepared Plate (Ø 90 mm)) Culture medium for the cultivation and enumeration of Enterobacteriaceae. Bile Salts Mixture Crystal Violet Neutral Red D(+)-Glucose Yeast Extract Gelatin Peptone Agar plates v XLD Agar (ISO 6579, ISO 19250, ISO 21567) (Prepared Plate (Ø 90 mm)) Culture medium for the isolation of Salmonella and Shigella. Ammonium Iron(III) Citrate Yeast Extract Lactose L-Lysine Phenol Red Saccharose Sodium Deoxycholate Sodium Thiosulfate D(+)-Xylose plates v PREPARED TUBES Letheen Broth (modified) (Prepared Tubes) Culture medium for the determination of the antimicrobial activity of quaternary ammonium compounds. Meat Extract Yeast Extract Lecithin Casein Peptone Meat Peptone Sodium Chloride Sodium Bisulfite Tween tubes v Peptone Water (Prepared Tubes) Medium for the cultivation of nonfastidious microorganisms, for the carbohydrate fermentation test and to carry out the Indole test. Casein Peptone tubes v Selenite Cystine Broth (Prepared Tubes) Medium for the enrichment of Salmonella. UN: 3077 IMDG: 9/III ADR: 9/III IATA: 9/III PAX: 956 CAO: 956 (E) 789 H: H332 H302 H373 H411 P: P260 P261 P264 P270 P271 P273 P301+P312 P304+P340 P3 12 P314 P330 P391 P501 Sodium Hydrogen Selenite L(-)-Cystine Lactose tri-sodium Phosphate Tryptone tubes v 21

22 SPS Agar (Prepared Tubes) Medium for the detection and enumeration of sulfite-reducing clostridia. Sodium Sulfite Polymixin B Sulfate Sodium Sulfadiazine Yeast Extract Iron(III) Citrate Casein Peptone Agar tubes v Tryptone Yeast Extract Agar (ISO 6222:1999) (Prepared Tubes) Medium for the enumeration of microorganism in water according to ISO 6222:1999 Yeast Extract Tryptone Agar tubes v PREPARED BOTTLES Buffered Peptone Water (ISO 6579, ISO 22964, ISO 6887, DIN 10181, 10160) (Prepared Bottles) Diluent agent to homogenize samples in microbiological analysis. Casein Peptone di-sodium Phosphate 12-hydrate mono-potassium Phosphate x 3 l v x 100 ml v Buffered Sodium Chloride-Peptone solution (Ph. Eur.) (Prepared Bottles) Diluent agent to homogenize samples in microbiological analysis. NC: Casein Peptone Potassium di-hydrogen Phosphate Sodium Chloride di-sodium Hydrogen Phosphate x 90 ml v Fraser 1/2 Listeria Broth (ISO :1996) (Prepared Bottles) Primary enrichment broth for Listeria monocytogenes. Esculin Yeast Extract Lithium Chloride Potassium di-hydrogen Phosphate Meat Peptone Sodium Chloride di-sodium Phosphate Tryptone Meat Extract Ammonium Iron(III) Citrate Nalidixic Acid Acryflavine x 450 ml v Peptone Water with neutralizing agents (Ph. Eur.) (Prepared Bottles) Neutralising solution in the dilution of samples with antimicrobial agents. Casein Peptone Lecithin (egg) Histidine Potassium di-hydrogen Phosphate Sodium Chloride di-sodium Hydrogen Phosphate Sodium Thiosulfate Tween x 450 ml v Tryptone Yeast Extract Agar (ISO 6222:1999) (Prepared Bottles) Medium for the enumeration of microorganisms according to ISO 6222:1999 Yeast Extract Tryptone x 100 ml v TSC Agar Base (ISO 7937) (Prepared Bottles) Culture medium for the detection and enumeration of Clostridium perfringens and other anaerobics in water, foods and other materials. Yeast Extract Iron(III) Citrate Soy Peptone Sodium Disulfite Tryptose Agar ph: 7.6 ± x 100 ml v 22 Products for Microbiology

23 Microbiology DIP SLIDES FOR HYGIENE CONTROL Slide PCA/PCA Culture medium for the total enumeration of aerobes. Composition (g/l) (Side 1 and 2): Tryptone Yeast Extract D(+)-Glucose TTC di-sodium Phosphate Phosphatidylcholine L-Histidine Sodium Thiosulfate Tween units v Slide PCA/RB Culture medium for the total enumeration of aerobes and for the enumeration of fungi and yeasts. Composition (g/l) (Side 1): Tryptone Yeast Extract D(+)-Glucose TTC di-sodium Phosphate Phosphatidylcholine L-Histidine Sodium Thiosulfate Tween Composition (g/l) (Side 2): Soy Peptone D(+)-Glucose Magnesium Sulfate Rose Bengal Chloramphenicol di-sodium Phosphate Phosphatidylcholine L-Histidine Sodium Thiosulfate Tween units v Slide PCA/VRBG Culture medium for the total enumeration of aerobes and enterobacteria. Composition (g/l) (Side 1): Tryptone Yeast Extract D(+)-Glucose TTC di-sodium Phosphate Phosphatidylcholine L-Histidine Sodium Thiosulfate Tween Composition (g/l) (Side 2): Yeast Extract Peptone Bile Salts nº D(+)-Glucose Neutral Red Crystal Violet di-sodium Phosphate Phosphatidylcholine L-Histidine Sodium Thiosulfate Tween Agar units v ACCESSORIES Analytical Funnel, sterilized, individually packed, 47 mm, 0.45 microns (Removable filter) 4AFW-045MC 50 units v Microbiological Monitor, sterilized, individually packed, 47 mm, 0.45 microns (Welded fixed filter) FMW-045MC 50 units v 2.5 L Anaerobiosis Jar ANJARRMC 1 unit v Nylon, three-port Filtration Manifold MAN03NYMC 1 unit v Nitrocellulose membranes, 48,3 mm, 0,45 microns, sterile NC-04548MC 100 filters v Because of the continuous updating of our catalog of products and presentations, kindly confirm product availability, through our website. 23

24 Dip Slides PanReac AppliChem dip slides are a suitable, easy-to-use and cheap system for the Microbiological Hygiene Control of both surfaces and solutions. Each dip slide has two faces coated with agar-based medium, with a usable area of 12 cm2 per face. These two faces can contain different media, thus meaning that twice as much information regarding microbial contamination can be obtained using a single device. The flexibility of the plastic strip allows samples to be taken at sites that are inaccessible to contact plates. They can also be used for the qualitative control of microbial contamination in liquids by simply immersing the dip slide in the sample. You can store it at room temperature for up to six months. The dipslides can be used by untrained personnel due to ease of use. The results can be quantified after incubation under the indicated conditions. The dip slide range is suitable for the majority of Hygiene Control applications in both the food industry and other industrial applications.. CFU/ml Measuring microbial contamination using PanReac AppliChem dip slides. Total aerobe count on PCA+TTC (CFU/ml) Analytical Funnels and Microbiological Monitors Microbiological analysis systems based on filtering for use in industries related to food, soft drinks, beer, wines, water and the pharmaceutical industry. Analytical Funnels The analytical funnels have a capacity of 100 ml and a removable membrane, ensuring flexibility for filtering and placing the filter on any culture medium plate. Ideal for use in the pharmaceutical industry, particularly for monitoring of process water. Available in individual sterile container and in 0.45 microns. Code AFW-045MC (50 units) Monitors The monitors are fitted with a stationary membrane next to the pad at the base of the funnel, forming an incubation unit, along with the cover. The monitors have a capacity of 100 ml and are used in combination with liquid culture medium. Ideal for use in laboratories for soft drinks, water, wines, etc. Code FMW-045MC (50 units) Oxidase Sticks Code Contains 50 test strips used for the easy and rapid detection of the cytochromeoxidase enzyme in microbiological diagnosis. Unlike the basic laboratory test where the tetramethyl-p-phenylenediamine reagent is highly unstable, PanReac AppliChem oxidase sticks have a stabilised reagent on the pad with a greater stability. 24 Products for Microbiology

25 Microbiology Surface Hygiene Control Contact Plates Staphylococci Fungi and yeasts Baird-Parker, Agar Sabouraud Glucose Agar+Chloramphenicol (Ph. Eur.) Code Description: S. aureus forms black colonies with a clear halo due to lecithinase activity. A Enterobacteriaceae Violet Red Bile Glucose Agar (VRBG) (Ph.Eur.) Code A B A - Blank plate. B - Contact surface. Incubation at 31 C/72 hours. Presence. Rose Bengal Chloramphenicol Agar Description: Enterobacteriaceae form pink-red colonies and surrounded by a reddish halo of precipitation. A - Blank plate B - Carton box (contact). Incubation at 22 C /5 days. Presence. Aerobic microorganisms B Code Description: Recommended medium for the control of fungi and yeasts on different surfaces. The presence of chloramphenicol inhibits bacterial growth, thus liberating development of fungi and yeast. Use of the Sabouraud medium supplemented with the antibiotic is recommended when there is a significant accompanying bacterial flora. Code Description: The yeast colonies appear rose-tinted, and fungi colonies have their characteristic features. This medium limits the extension of the colonies, thus allowing more objective counts on highly contaminated surfaces. TSA-Tween-Lecithin-Agar (Ph. Eur.) A B A - Blank plate. B - Warehouse Incubation 37 C/72 hours Presence. Code Description: Medium recommended by the European Pharmacopoeia for the total aerobic count. The presence of Lecithin and Tween neutralizes the antimicrobial activity of certain products. Plate Count Agar (PCA) (ISO 4833:2003) A A - Blank plate. B - Packing room (contact). Incubation a 37 C/72 hours Presence. B Code Description: Medium recommended by APHA for enumeration of mesophilic aerobic bacteria. Tryptone Soy Agar (TSA) (Ph. Eur.) Code

26 Chromogenic media Industry application chart Code Description Supplement Use Reference Method Microorganism SCO/778/2009 of 17 March 2009 E. coli, coliforms CCA Coliforms, Chromogenic Agar (Prepared Plate (Ø 55 mm)) Selective detection Chromogenic E. coli Agar Selective detection Chromogenic Salmonella Agar Isolation Salmonella Salmonella Chromogenic Media (Prepared Plate (Ø 90 mm)) Isolation Salmonella TBX Agar (ISO ,3:2000) Selective detection / enumeration ISO ,3 E. coli TBX Agar (ISO ,3:2000) (Prepared Plate (Ø 90 mm)) Selective detection / enumeration IISO ,3 E. coli Listeria Chromogenic Agar (ISO 11290:2004) Listeria Chromogenic Agar (ISO :2004) (Prepared Plate (Ø 90 mm)) Selective detection / enumeration Selective detection / enumeration E. coli, coliforms ISO ISO Listeria monocytogenes Listeria monocytogenes Coliforms Salmonella E. coli Gram Lauryl Sulfate Chromogenic Broth Candida Chromogenic Agar Enterobacter Sakazakii Chromogenic Agar (ISO 22964) Isolation ISO Enterobacter sakazakii E. coli Enterobacter Sakazakii Chromogenic Agar (ISO 22964) (Prepared Plate (Ø 90 mm)) Isolation IISO Enterobacter sakazakii E. coli E. coli O157:H7 Chromogenic Agar Base Detection Lauryl Sulfate Chromogenic Broth 26 Products for Microbiology Candida albicans Candida krusei Candida tropicalis Selective detection / enumeration Selective detection / enumeration Candida Chromogenic Agar E. coli 0157.H7 E. coli O157:H7 Chromogenic Agar Base TBX Agar (ISO ,3:2001)

27 Microbiology Meat & Fish Water & Beverage Dairy products Bakery Processed food Beer industry Wastewater Cosmetic industry Pharmaceutical industry Other Chromogenic E. coli Agar CCA Coliforms, Chromogenic Agar according to SCO/778/2009 Chromogenic Salmonella Agar Listeria Chromogenic Agar (ISO 11290:2004) Enterobacter Sakazakii Chromogenic Agar 27

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