FOOD Microbiology Testing
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- Ronald Anthony
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1 Microorganisms Involved in processing and spoilage of food 1. Acid Producing Microorganisms B907 B908 B909 BF028 B390 B985 B464 B189 B1099 B536 B575 APT Agar APT Broth ASLA Agar Base Propionibacteria Growth Supplement Acetobacter Agar (Glucose) Chopped Liver Broth Dextrose Tryptone Agar Eugonic Agar Eugonic Broth HYA Agar Lactic Agar B792 B576 B844 B219 B841 B1085 B858 B626 B378 B750 Lactic Streak Agar (Reddy's Differential Agar, Modified) Lactobacillus Bulgaricus Agar Base Lactobacillus MRS Agar (MRS Agar) Lactobacillus MRS Broth (MRS Broth) Lactobacillus Selection Agar Base Lee's Agar M17 Agar Base Modified Rogosa Agar (M16 Agar) Rogosa SL Agar w/0.15% Oxgall Streptococcus Lactis Differential Agar Base 2. Aciduric Acid Thermophilic Flat Sour Sporeformers B914 B464 B170 B171 B1004 Acid Broth Dextrose Tryptone Agar Dextrose Tryptone Agar, Modified Detrose Tryptone Broth, Dextrose Tryptone Broth, Modified B225 B318 B034 B331 Litmus Milk Shapton Medium Nutrient Broth, ph 6.9, w/o NaCl Thermoacidurans Agar 3. Halophilic Microorganisms B1055 B1056 B839 Halophilic Agar Halophilic Broth Sea Water Agar (Twin Pack) B797 B522 Tryptone Soya Agar w/added NaCl Nutritive Caseinate Agar 4. Lipolytic Microorganisms B188 B076 Spirit Blue Agar Tributyrin Agar Base w/o Tributyrin BF098 Tributyrin 28
2 5. Mesophilic Sporeforming (Aerobic) B464 Dextrose Tryptone Agar B790 Tryptone Glucose Extract Agar (Tryptone Glucose Yeast Extract Agar) 6. Mesophilic Sporeforming (Anaerobic) B202 B1205 B519 B493 B331 Heart Infusion Broth Liver Broth Orange Serum Agar PE-2 Medium Thermoacidurans Agar B776 B1103 B061 BF091 Thioglycollate Agar Tryptone Glucose Yeast Extract Broth Tryptose Cycloserine Dextrose Agar Base T.S.C.Supplement 7. Osmophilic Microorganisms B038 B799 Fluid Sabouraud Medium MY 40 Agar (Osmophilic Agar) B798 B036 MY 40G Agar (Osmophilic Glucose Agar) Potato Dextrose Agar 8. Pectinolytic Microorganisms B852 MP-5 Medium B851 MP-7 Medium 9. Proteolytic Microorganisms B522 B724 Nutritive Caseinate Agar Skim Milk Agar B737 B1158 Standard Methods Caseinate Agar Calcium Caseinate Agar 10. Psychrotrophic Microorganisms B477 Crystal Violet Tatrazolium Agar Base BF044 TTC Solution 1% B249 Malt Extract Agar Base (w/mycological Peptone) BF079 B298 B036 B039 10% Lactic Acid Solution Plate Count Agar (Standard MethodsAgar) Potato Dextrose Agar Soyabean casein Digest Agar 29
3 11. Sulphide Spoilage Sporeformers B208 Iron Suphite Agar B327 Sulphite Agar 12. Thermoduric Microorganisms B907 APT Agar B298 Plate Count Agar (Standard MethodsAgar) 13. Thermophilic Flat Sour Sporeforming B1205 B493 B168 B464 Liver Broth PE-2 Medium Dextrose Peptone Broth Dextrose Tryptone Agar B171 B170 B879 Dextrose Tryptone Broth Dextrose Tryptone Agar, Modified M-Dextrose Tryptone Broth 14. Total Bacteria Count I. Colony Count Method B914 Acid Broth B907 APT Agar B844 Lactobacillus MRS Agar (MRS Agar) B1235 Neutralising Buffer B297 B298 B381 B1103 Phosphate Buffer, APHA, ph7.2 Plate Count Agar (Standard MethodsAgar) Ringer Salt Solution Powder Tryptone Glucose Yeast Extract Broth 15. Total Yeast and Mold Count B905 BF109 B906 BF027 B846 BF004 B130 B1009 B1010 BF004 APRY Agar Base Potassium Sorbate 10% (10ml per vial) APRY Broth Base Chlortetracycline Selective Supplement Aspergillus Differentiation Medium Base Chloramphenicol Selective Supplement Buffered Yeast Agar Dichloran Glycerol Medium Base Dichloran Medium Base w/rose Bengal Chloramphenicol Selective Supplement B408 B248 B279 BF086 B1241 BF086 B459 BF027 B036 Fingobiotic Agar (Mycolbio Agar) Malt Agar Oxytetra Glucose Yeast Agar Base (OGYE Agar Base) Oxytetra Selective Supplement Oxytetra Glucose Yeasts Agar Base w/biotin Oxytetra Selective Supplement Pentachloro Rose Bengal Yeast Extract Agar Base (PRYES Agar) Chlortetracycline Selective Supplement Potato Dextrose Agar 30
4 15. Total Yeast and Mold Count B645 B307 BF004 Potato Dextrose Rose Bengal Agar Rose Bengal Agar Base Chloramphenicol Selective Supplement B703 B820 Rose Bengal Chloramphenicol Agar YXT Agar Base Indicator Microorganisms and Pathogens: 1. Aeromonas hydrophila B918 BF001 B919 BF095 B398 B943 B481 B565 B505 B1079 Aeromonas Isolation Medium Base Aeromonas Selective Supplement Aeromonas Starch DNA Agar Base Ampicillin Supplement Alkaline Peptone Water Bile Salts Brilliant Green Starch Agar DNase Test Agar w/toludine Blue Inositol Brilliant Green Bile Agar (Plesiomonas Differential Agar) Glutamate Starch Phenol Red Agar Base Kaper's Medium B1097 B550 B035 B388 BF083 B702 BF094 B704 BF095 B039 B040 Lachica's Medium Base Nutrient Agar Peptone Water RS Medium Novobiocin Supplement Rippey - Cabelli Agar Base Rippey - Cabelli Selective Supplement SA Agar Base Ampicillin Supplement Soyabean Casein Digest Agar Soyabean Casein Digest Medium 2. Bacillus cereus B409 B938 BF005 BF003 B562 B1188 BF003 BF005 B672 BF003 B.C.Motility Test Medium Bacillus Cereus Agar Base Polymixin B Selective Supplement Egg Yolk Emulsion ISP Medium No.7 (Tyrosine Agar) KG Agar Base Egg Yolk Emulsion Polymixin B Selective Supplement MYP Agar Base (Phenol Red Egg Yolk Polymixin Agar Base) Egg Yolk Emulsion BF005 Polymixin B Selective Supplement B629 Modified MYP Agar Base BF005 Polymixin B Selective Supplement BF003 Egg Yolk Emulsion B631 Modified V.P. Broth B270 I Nitrate Broth B654 Nutrient Agar, ph 6.8 B286 Phenol Red Dextrose Broth B039 Soyabean Casein Digest Agar B040 Soyabean Casein Digest Medium B064 I Tryptone Yeast Extract Agar w/bcp 31
5 3. Campylobacter species I. Non Selective Media B125 BF016 B126 Brucella Agar Base Campylobacter Growth Supplement Brucella Broth Base BF016 Campylobacter Growth Supplement Enrichment Supplement II. Selective Isolation Media B116 BF014 B125 BF013 B964 BF013 BF014 B988 BF013 Blood Agar Base No.2 Campylobacter Supplement - III (Skirrow) Brucella Agar Base Campylobacter Supplement - I (Blaser - Wang) Campylobacter Agar Base Campylobacter Supplement - I (Blaser - Wang) Campylobacter Supplement - III (Skirrow) Christopher's Semisolid Brucella Medium Base Campylobacter Supplement - I (Blaser - Wang) B994 Columbia Blood Agar Base BF013 Campylobacter Supplement - I (Blaser - Wang) BF014 Campylobacter Supplement - III (Skirrow) B1080 Karmali Campylobacter Agar Base BF112 Campylobacter Selective Supplement (Karmali) BF113 Campylobacter Selective Supplement w/hemin (Karmali) B657 Preston Agar Base BF033 Campylobacter Supplement - IV (Preston) III. Enrichment Broth B966 BF033 B967 B017 B1013 BF050 B201 B202 B1086 B274 Campylobacter Enrichment Broth Base (Preston Enrichment Broth Base) Campylobacter Supplement - IV (Preston) Campylobacter Nitrate Broth Cary-Blair Medium Base (Transport Medium w/o Charcoal) Doyle's Enrichment Broth Base Doyle's Antibiotic Supplement Heart Infusion Agar Heart Infusion Broth King's Of Medium Base Nutrient Broth B463 BF087 BF032 B196 B089 B048 B031 B029 Park and Sanders Enrichment Broth Base Park and Sanders Selective Supplement I Park and Sanders Selective Supplement II Smibert's Semisolid Brucella Medium TMAO Medium (Trimethylamine-N-Oxide Medium) Triple Sugar Iron Agar Wang's Semisolid Medium Wesley Broth Base Campylobacter Selective Supplement 32
6
7 6. Coliforms - E. coli and it's Toxin 7. Enterococci B107 B422 B424 BF011 B435 B121 B1156 Azide Dextrose Broth Bile Esculin Agar Bile Esculin Agar Base Esculin Brain Heart Infusion w/6.5% NaCl Brain Heart Infusion Broth CAE (Citrate Azide Enterococcus) Agar Base BF044 TTC Solution 1% B178 EE Broth, Mossel B1019 EE Broth, Modified B1032 FGTC Agar Base BF111 Amylose Azure BF053 FGTC Antibiotic Supplement BF054 Sodium Bicarbonate Solution 34
8 7. Enterococci B1050 BF063 BF054 B1133 BF130 B1145 GTC Agar Base GTC Supplement Sodium Bicarbonate Solution Kanamycin Esculin Azide Agar Base Kanamycin Sulphate Selective Supplement Kanamycin Esculin Azide Broth Base BF130 Kanamycin Sulphate Selective Supplement B1077 KF Streptococcal Agar Base BF044 TTC Solution 1% BF067 Bromo Cresol Purple B406 M-Enterococcus Agar Base B1277 Violet Red Bile Glucose Agar w/lactose (Agar Medium F) 8. Listeria species B121 B441 B968 B994 B963 I BF117 BF002 B1045 BF055 BF117 B1190 BF136 B893 B892 BF134 BF135 B891 BF072 B098 BF072 Brain Heart Infusion Broth Bromo Cresol Purple Broth Base Carbohydrate Consumption Broth Base Columbia Blood Agar Base Fraser Broth Base Fraser Selective Supplement Fraser Supplement Fraser Secondary Enrichment Broth Base Fraser Enrichment Supplement Fraser Selective Supplement LPM Agar Base Moxalactam Supplement Listeria Enrichment Broth, Modified Listeria Enrichment Medium Base (UVM) Listeria UVM Supplement I Listeria UVM Suppement II Listeria Identification Agar Base (PALCAM) Listeria Selective Supplement Listeria Identification Broth Base (PALCAM) Listeria Selective Supplement (PALCAM) B888 Listeria Selective Broth Base BF075 Listeria Selective Supplement II BF075 I Listeria Selective Supplement II B890 I Listeria Motility Medium B889 Listeria Oxford Medium Base BF073 Oxford Listeria Supplement BF074 Listeria Moxalactam Supplement B543 MR-VP Medium (Glucose Phosphate Broth) B636 Modified McBride Listeria Agar Base BF080 McBride Listeria Supplement B597 Motility Test Medium B270 I Nitrate Medium B274 Nutrient Broth B039 Soyabean Casein Digest Agar B040 Soyabean Casein Digest Medium B048 Triple Sugar Iron Agar B052 I Tryptone Soya Yeast Extract Agar B1275 Universal Pre-enrichment Broth B496 Urea Broth Base 9. Plesiomonass shigelloides : B398 B016 Alkaline Peptone Water Brilliant Green Bile Agar B027 Fluid Tetrathionate Medium w/o Iodine & BG (Tetrathionate Broth Base w/o Iodine & BG) 35
9 9. Plesiomonass shigelloides : B1072 B238 B472 Inositol Gelatin Medium MacConkey Agar w/0.15% bile salts, CV & NaCl PL Agar B035 B565 B310 B048 Peptone Water Plesiomonas Differential Agar S S Agar Triple Sugar Iron Agar 10. Salmonella species I. Non Selective Media for Enrichment & Propagation B121 B024 B197 B316 B220 Brain Heart Infusion Broth Fluid Lactose Medium GN Broth, Hajna Lactose Broth Lauryl Sulphate Broth (Lauryl Tryptose Broth) B577 B274 B040 B1275 M-Broth Nutrient Broth Soyabean Casein Digest Medium Universal Pre-enrichment Broth II. Selective Peptone Water w/nacl B129 BW129 B026 B027 B1224 B628 BF137 Buffered Peptone Water Buffered Peptone Water Selenite Cystine Broth (Twin Pack) Fluid Tetrathionate Medium w/o Iodine & BG (Tetrathionate Broth Base w/o Iodine & BG) Mannitol Selenite Broth w/brilliant Green (Twin Pack) Modified Rappaport Vassiliadis Medium IMRV / RV Selective Supplement B386 B1252 B717 B719 B1257 B769 B083 B796 Rappaport Vassiliadis Medium Rappaport Vassiliadis Soyabean Meal Broth (RVSM) Selenite Broth Base w/o Selenite Selenite Cystine Broth Base Semisolid RV Medium Base Taurocholate Broth Tetrathionate CV Enrichment Broth Tryptone Water Broth w/bcp III. Selective Isolation Media B1147 Bismuth Sulphite Agar B429 Bismuth Sulphite Agar, Modified B016 Brilliant Green Bile Agar B123 Brilliant Green Agar Base, Modified BF020 Sulpha Supplement B440 Brilliant Green Sulpha Agar B1052 Gassner Lactose Agar B203 Hektoen Enteric Agar B238 MacConkey Agar w/0.15% Bile Salts, CV & NaCl B310 S S Agar B370 B365 B345 B788 B350 B1277 B814 I B361 S S Agar, Modified Salmonella Differential Agar (Twin Pack) Salmonella Differential Agar, Modified (Twin Pack) Tryptone Bile Agar Violet Red Bile Agar Violet Red Bile Glucose Agar w/lactose (Agar Medium F) Violet Red Bile Glucose Agar w/o Lactose XLD Agar 36
10 IV. Media for Biochemical Characterisation of Isolate B441 Bromo Cresol Purple Broth Base B1058 Hemmes Medium Base B220 Lauryl Sulphate Broth (Lauryl Tryptose Broth) B231 Lysine Decarboxylase Broth B232 Lysine Iron Agar B596 Lysine Iron Cystine Broth Base BF077 Novobiocin Selective Supplement B543 MR-VP Medium (Glucose Phosphate Broth) B245 MacConkey Broth Purple w/bcp B246 Malonate Broth B579 Motility Test Medium B284 Phenol Red Broth Base B319 Simmons Citrate Agar B040 Soyabean Casein Digest Medium B048 Triple Sugar Iron Agar B1127 Tryptone Broth (Tryptone Water) B496 Urea Broth Base 11. Shigella species B913 Acetate Differential Agar B197 GN Borht, Hajna B203 Hektoen Enteric Agar B1058 Hemmes Medium Base B543 MR-VP Medium (Glucose Phosphate Broth) B238 MacConkey Agar w/0.15% bile salts, CV & NaCl B246 Malonate Broth B634 Moeller Decarboxylase Broth w/lysine HCl B635 Moeller Decarboxylase Broth w/ornithine HCl B579 Motility Test Medium B550 Nutrient Agar B274 Nutrient Broth B286 Phenol Red Dextrose Broth B675 Phenol Red Dulcitol Broth B295 Phenol Red Sucrose Broth B89 Phenol Red Lactose Broth B310 S S Agar B1286 Shigella Broth Base BF138 Shigella Selective Supplement B319 Simmons Citrate Agar B040 Soyabean Casein Digest Medium B048 Triple Sugar Iron Agar B064 I Tryptone Water Broth w/bcp B496 Urea Broth Base B361 XLD Agar 12. Staphylococcu aureus B013 Baird Parker Agar Base BF007 Egg Yolk Tellurite Emulsion BF003 Egg Yolk Emulsion BF008 Potassium Tellurite 3.5% BF009 B P Sulpha Supplement B120 Brain Heart Infusion Agar B121 Brain Heart Infusion Broth B140 Chapman Stone Agar B198 Giolitti-Cantoni Broth Base BF008 Potassium Tellurite 3.5% B567 K.R.A.N.E.P. Agar Base BF003 Egg Yolk Emulsion 37
11 12. Staphylococcu aureus B672 BF005 BF003 B053 BF003 B622 B629 BF005 BF003 MYP Agar Base (Phenol Red Egg Yolk Polumixin Agar Base) Polymixin B Selective Supplement Egg Yolk Emulsion Mannitol Salt Agar Base Egg Yolk Emulsion Milk Salt Agar Base Modified MYP Agar Base Polymixin B Selective Supplement Egg Yolk Emulsion B039 Soyabean Casein Digest Agar B040 Soyabean Casein Digest Medium B329 TPEY Agar Base BF003 Egg Yolk Emulsion BF010 Potassium Tellurite 1% B770 Tellurite Glycine Agar Base BF010 Potassium Tellurite 1% B079 Toludine Blue DN Agar B352 Vogel-Johnson Agar Base w/o Tellurite (V.J.Agar) BF010 Potassium Tellurite 1% 13. Vibrio species B398 Alkaline Peptone Water B017 Cary-Blair Medium Base (Transport Medium w/o Charcoal) B455 Casein Hydrolysate Yeast Extract Broth (CAYE) B960 CPC Agar Base BF031 CPC Supplement B532 Gelatin Agar B535 Gelatin Phosphate Salt Agar (GPS Agar) B1053 Gelatin Salt Agar B504 Glucose Salt Teepol Broth (Twin Pack) B201 Heart Infusion Agar B202 Heart Infusion Broth B1062 I High Salt Nutrient Agar B1063 I High Salt Peptone Yeast Extract Agar B554 Horie Arabinose Ethyl Violet Broth B212 Kligler Iron Agar B579 B263 B375 B315 BF005 B039 B040 B042 B768 B048 B1105 B372 B387 BW356 Motility Test Medium Mueller Hinton Agar SDS Agar (Sodium Dodecyl Sulphate Polymixin Sucrose Agar) Salt Polymixin Broth Base Polymixin B Selective Supplement Soyabean Casein Digest Agar Soyabean Casein Digest Medium TCBS Agar TN Agar Triple Sugar Iron Agar Tryptone Soya Salt Agar w/magnesium Sulphate VP Medium Vibrio Parahaemolyticus Sucrose Agar Wagatsuma Agar Base 14. Yersinia species B424 BF011 B441 B444 Bile Esculin Agar Base Esculin Bromo Cresol Purple Broth Base CAL Agar (Cellobiose Arginine Lysine Agar) B445 CAL Broth (Cellobiose Arginine Lysine Broth) B961 CRAMP Agar Base B022 EMB Agar B1068 I ITC Broth Base (TTC Broth Base) 38
12 14. Yersinia species BF065 B212 B853 B128 B238 B628 B633 B634 B635 B579 B270 I B662 I Ticarcillin Supplement Kligler Iron Agar MOX Agar Buffered Glucose Broth MacCocnkey Agar w/0.15% Bile Salts, CV & NaCl Modified Rappaport Vassiliadis Medium Moeller Decarboxylase Broth w/arginine HCl Moeller Decarboxylase Broth w/ Lysine HCl Moeller Decarboxylase Broth w/ Ornithine HCl Motility Test Medium Nitrate Broth PSB Broth Base B662 PSTA Enrichment Broth base B035 Peptone Water B658 Pre-Enrichment Broth Base B319 Simmons Citrate Agar B040 Soyabean Casein Digest Medium B048 Triple Sugar Iron Agar B064 I Tryptone Water Broth w/bcp BW347 Urea Agar Base (Christensen ) (Autoclavable) B822 Yeast Extract Rose Bengal Broth Base B003 I Yersinia Identification Broth Base B002 I Yersinia Isolation Agar B001 I Yersinia Selective Agar Base BF102 Yersinia Selective Supplement 39
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